Ppp4r3b cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ppp4r3b
Entrez Gene ID	360993
Full Name	protein phosphatase 4, regulatory subunit 3B
Synonyms	RGD1309450,Smek2
General protein information	Preferred Names protein phosphatase 4, regulatory subunit 3B Names serine/threonine-protein phosphatase 4 regulatory subunit 3B SMEK homolog 2, suppressor of mek1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 14 Map Location: 14q22

mRNA and Protein(s)

mRNA	Protein	Name
NM_001108367.1	NP_001101837.1	serine/threonine-protein phosphatase 4 regulatory subunit 3B

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	SMEK2	XP_003309065.1
Bos taurus (cattle)	SMEK2	NP_001178079.1
Gallus gallus (chicken)	SMEK2	XP_424305.3
Drosophila melanogaster (fruit fly)	flfl	NP_731849.2
Canis lupus familiaris (dog)	SMEK2	XP_005626173.1
	SMEK2	XP_001112386.2
Rattus norvegicus (Norway rat)	Smek2	NP_001101837.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003402	XP_311690.5
Homo sapiens (human)	SMEK2	NP_001116436.2
Mus musculus (house mouse)	Smek2	NP_598795.2
Danio rerio (zebrafish)	smek2	NP_956295.2
Xenopus tropicalis (tropical clawed frog)	smek2	NP_001005004.1

Gene Ontology
Bibliography

Related articles in PubMed

Suppressor of MEK null (SMEK)/protein phosphatase 4 catalytic subunit (PP4C) is a key regulator of hepatic gluconeogenesis.
Yoon YS, Lee MW, Ryu D, Kim JH, Ma H, Seo WY, Kim YN, Kim SS, Lee CH, Hunter T, Choi CS, Montminy MR, Koo SH
Proceedings of the National Academy of Sciences of the United States of America107(41)17704-9(2010 Oct)

Identification of SMEK2 as a candidate gene for regulation of responsiveness to dietary cholesterol in rats.
Asahina M, Haruyama W, Ichida Y, Sakamoto M, Sato M, Imaizumi K
Journal of lipid research50(1)41-6(2009 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

SMEK2 is a candidate gene for regulation of responsiveness to dietary cholesterol in rats
Title: Identification of SMEK2 as a candidate gene for regulation of responsiveness to dietary cholesterol in rats.

The following Ppp4r3b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ppp4r3b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa07205	NM_001108367.1 Latest version!	Rattus norvegicus protein phosphatase 4, regulatory subunit 3B (Ppp4r3b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ppp4r3b ( NM_001108367.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001101837.1 Rattus norvegicus protein phosphatase 4, regulatory subunit 3B (Ppp4r3b), mRNA.

Price & Availability↑

CloneID	ORa07205
Clone ID Related Accession (Same CDS sequence)	NM_001108367.1
Accession Version	NM_001108367.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2463bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Rattus norvegicus(Norway rat)
Product	serine/threonine-protein phosphatase 4 regulatory subunit 3B
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CH473996.1. On or before Oct 4, 2007 this sequence version replaced XM_001053322.1, XM_001058097.1. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGTCGGATA CACGGCGGCG AGTGAAGGTC TATACCCTGA ACGAAGACCG GCAATGGGAC 
GACCGAGGCA CCGGGCACGT CTCCTCCACT TACGTAGAGG AGCTCAAAGG GATGTCGCTG 
CTGGTTCGAG CAGAGTCCGA CGGATCTCTA CTCTTGGAGT CAAAGATAAA TCCAAACACT 
GCATATCAGA AGCAGCAGGA TACATTAATT GTTTGGTCAG AAGCTGAAAA CTATGATTTG 
GCTCTAAGTT TTCAAGAAAA AGCTGGCTGT GATGAAATTT GGGAAAAAAT TTGTCAAGTT 
CAAGGTAAGG ACCCATCAGT AGAAGTCACA CAGGACCTCA TTGATGAATC AGAAGAAGAA 
CGATTTGAAG AAATGCCAGA AACTAGTCAT CTAATTGACC TGCCCACATG TGAACTCAGT 
AAACTTGAAG AGATTGCTGA CTTAGTTACC TCAGTTCTCT CCTCACCTAT CCGTAGGGAA 
AAGCTGGCTC TGGCCTTGGA AAATGAAGGC TATATCAAAA AACTGCTGCA GCTGTTCCAG 
GCTTGTGAGA ACCTAGAAAA CACTGAAGGC TTACACCAGC TGTATGAGAT TATTAGAGGA 
ATCTTATTCC TAAATAAGGC AACTCTTTTT GAGGTAATGT TTTCTGATGA GTGTATCATG 
GATGTCGTGG GATGCCTTGA ATATGATCCT GCTTTGGCCC AGCCAAAAAG ACATAGAGAA 
TTCTTGACTA AAACTGCAAA GTTTAAGGAA GTTATACCAA TAACAGACTC GGAACTAAGG 
CAAAAAATAC ATCAGACTTA CAGGGTACAA TACATTCAGG ACATCATTTT ACCTACACCA 
TCTGTTTTTG AAGAGAATTT CCTTTCTACT CTTACATCTT TTATTTTCTT CAACAAAGTT 
GAAATAGTCA GCATGCTGCA GGAAGATGAG AAATTTTTGT CTGAAGTTTT TGCACAATTA 
ACAGATGAGG CTACAGATGA TGATAAACGG CGCGAACTGG TTAATTTCTT CAAAGAATTT 
TGTGCATTTT CTCAGACATT GCAACCTCAG AACAGGGATG CTTTTTTCAA AACCTTGGCA 
AAATTGGGAA TTCTTCCAGC TCTTGAAATT GTAATGGGCA TGGATGATTT GCAAGTCAGA 
TCAGCTGCTA CAGATATATT TTCTTATCTG GTAGAATTTA GTCCATCTAT GGTACGAGAA 
TTTGTGATGC AAGAAGCCCA GCAGAGTGAT GATGATATAC TTCTCATTAA CGTGGTGATT 
GAACAAATGA TTTGTGATAC TGACCCTGAG CTAGGAGGTG CTGTTCAACT AATGGGGCTT 
CTTCGTACTC TAATTGATCC AGAGAACATG TTGGCTACAA CTAATAAAAC TGAAAAGAGT 
GAGTTCTTAA ATTTCTTCTA CAATCATTGT ATGCATGTTC TCACAGCTCC ACTCTTGACC 
AATACATCAG AAGACAAATG TGAGAAAGAT AATATGCTTG GATCTAACAA AACCAACACA 
ATTTGTCCTG ATAATTATCA AACAGCACAG CTACTTGCCT TAATTTTAGA ATTACTCACA 
TTTTGTGTGG AACATCACAC ATACCATATA AAAAACTACA TCATGAACAA GGATTTGCTA 
AGAAGAGTCT TGGTGTTGAT GAATTCAAAG CATACTTTTC TGGCCTTGTG TGCTCTTCGT 
TTTATGAGGC GAATAATTGG CCTTAAGGAT GAGTTTTATA ATCGTTACAT CACCAAAGGA 
AATCTTTTTG AACCAGTTAT AAATGCCCTT TTGGATAATG GAACTCGGTA CAATCTATTA 
AATTCAGCTG TTATTGAATT GTTTGAATTT ATAAGAGTGG AAGATATCAA GTCTCTCACT 
GCACATATAG TTGAAAACTT TTATAAAGCA CTTGAATCGA TTGAATATGT TCAGACATTC 
AAAGGATTGA AGACTAAATA TGAACAAGAA AAAGACAGAC AAAATCAGAA ACTGAACAGT 
GTGCCATCTA TATTGCGTAG TAACCGATTT CGCAGAGATG CAAAAGCCTT GGAAGAGGAT 
GAAGAAATGT GGTTTAATGA AGATGATGAT GAAGAAGGAA AGTCAGTTGT AACACCAGTT 
GAAAAAACTA AGACTGAAGA TGATTTTCCA GATAGTTACG AAAAATTTAT GGAGACTAAA 
AAAGCAAAAG AAAGTGAAGA CAAGGAAAAC CTTCCCAAAA GGACATCTTC TGGTGGCTTC 
AAATTTACTT TCTCCCATTC CCCCAGTGCT GCTAATGGAA CAAACAGTAC AAACAGTAAG 
TCTGTAGTGG CTCAGACAAC ACCAGCAAGT TCTAATGGGT CCTCTTCCAA AACTACAAAC 
TTGGCTACAT CAGTGACAGC CACTAAGGGA AGTTTGGTTG GCTTAGTGGA CTATCCAGAT 
GATGAAGAGG AAGATGAGGA AGAAGAATCA TCCCCCAGAA AAAGACCTCG CCTTGGCTCA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001101837.1
CDS	348..2810
Translation	MSDTRRRVKVYTLNEDRQWDDRGTGHVSSTYVEELKGMSLLVRAESDGSLLLESKINPNTAYQKQQDTLIVWSEAENYDLALSFQEKAGCDEIWEKICQVQGKDPSVEVTQDLIDESEEERFEEMPETSHLIDLPTCELSKLEEIADLVTSVLSSPIRREKLALALENEGYIKKLLQLFQACENLENTEGLHQLYEIIRGILFLNKATLFEVMFSDECIMDVVGCLEYDPALAQPKRHREFLTKTAKFKEVIPITDSELRQKIHQTYRVQYIQDIILPTPSVFEENFLSTLTSFIFFNKVEIVSMLQEDEKFLSEVFAQLTDEATDDDKRRELVNFFKEFCAFSQTLQPQNRDAFFKTLAKLGILPALEIVMGMDDLQVRSAATDIFSYLVEFSPSMVREFVMQEAQQSDDDILLINVVIEQMICDTDPELGGAVQLMGLLRTLIDPENMLATTNKTEKSEFLNFFYNHCMHVLTAPLLTNTSEDKCEKDNMLGSNKTNTICPDNYQTAQLLALILELLTFCVEHHTYHIKNYIMNKDLLRRVLVLMNSKHTFLALCALRFMRRIIGLKDEFYNRYITKGNLFEPVINALLDNGTRYNLLNSAVIELFEFIRVEDIKSLTAHIVENFYKALESIEYVQTFKGLKTKYEQEKDRQNQKLNSVPSILRSNRFRRDAKALEEDEEMWFNEDDDEEGKSVVTPVEKTKTEDDFPDSYEKFMETKKAKESEDKENLPKRTSSGGFKFTFSHSPSAANGTNSTNSKSVVAQTTPASSNGSSSKTTNLATSVTATKGSLVGLVDYPDDEEEDEEEESSPRKRPRLGS

Target ORF information:

RefSeq Version	NM_001108367.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus protein phosphatase 4, regulatory subunit 3B (Ppp4r3b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001108367.1

ORF Insert Sequence:

ATGTCGGATA CACGGCGGCG AGTGAAGGTC TATACCCTGA ACGAAGACCG GCAATGGGAC 
GACCGAGGCA CCGGGCACGT CTCCTCCACT TACGTAGAGG AGCTCAAAGG GATGTCGCTG 
CTGGTTCGAG CAGAGTCCGA CGGATCTCTA CTCTTGGAGT CAAAGATAAA TCCAAACACT 
GCATATCAGA AGCAGCAGGA TACATTAATT GTTTGGTCAG AAGCTGAAAA CTATGATTTG 
GCTCTAAGTT TTCAAGAAAA AGCTGGCTGT GATGAAATTT GGGAAAAAAT TTGTCAAGTT 
CAAGGTAAGG ACCCATCAGT AGAAGTCACA CAGGACCTCA TTGATGAATC AGAAGAAGAA 
CGATTTGAAG AAATGCCAGA AACTAGTCAT CTAATTGACC TGCCCACATG TGAACTCAGT 
AAACTTGAAG AGATTGCTGA CTTAGTTACC TCAGTTCTCT CCTCACCTAT CCGTAGGGAA 
AAGCTGGCTC TGGCCTTGGA AAATGAAGGC TATATCAAAA AACTGCTGCA GCTGTTCCAG 
GCTTGTGAGA ACCTAGAAAA CACTGAAGGC TTACACCAGC TGTATGAGAT TATTAGAGGA 
ATCTTATTCC TAAATAAGGC AACTCTTTTT GAGGTAATGT TTTCTGATGA GTGTATCATG 
GATGTCGTGG GATGCCTTGA ATATGATCCT GCTTTGGCCC AGCCAAAAAG ACATAGAGAA 
TTCTTGACTA AAACTGCAAA GTTTAAGGAA GTTATACCAA TAACAGACTC GGAACTAAGG 
CAAAAAATAC ATCAGACTTA CAGGGTACAA TACATTCAGG ACATCATTTT ACCTACACCA 
TCTGTTTTTG AAGAGAATTT CCTTTCTACT CTTACATCTT TTATTTTCTT CAACAAAGTT 
GAAATAGTCA GCATGCTGCA GGAAGATGAG AAATTTTTGT CTGAAGTTTT TGCACAATTA 
ACAGATGAGG CTACAGATGA TGATAAACGG CGCGAACTGG TTAATTTCTT CAAAGAATTT 
TGTGCATTTT CTCAGACATT GCAACCTCAG AACAGGGATG CTTTTTTCAA AACCTTGGCA 
AAATTGGGAA TTCTTCCAGC TCTTGAAATT GTAATGGGCA TGGATGATTT GCAAGTCAGA 
TCAGCTGCTA CAGATATATT TTCTTATCTG GTAGAATTTA GTCCATCTAT GGTACGAGAA 
TTTGTGATGC AAGAAGCCCA GCAGAGTGAT GATGATATAC TTCTCATTAA CGTGGTGATT 
GAACAAATGA TTTGTGATAC TGACCCTGAG CTAGGAGGTG CTGTTCAACT AATGGGGCTT 
CTTCGTACTC TAATTGATCC AGAGAACATG TTGGCTACAA CTAATAAAAC TGAAAAGAGT 
GAGTTCTTAA ATTTCTTCTA CAATCATTGT ATGCATGTTC TCACAGCTCC ACTCTTGACC 
AATACATCAG AAGACAAATG TGAGAAAGAT AATATGCTTG GATCTAACAA AACCAACACA 
ATTTGTCCTG ATAATTATCA AACAGCACAG CTACTTGCCT TAATTTTAGA ATTACTCACA 
TTTTGTGTGG AACATCACAC ATACCATATA AAAAACTACA TCATGAACAA GGATTTGCTA 
AGAAGAGTCT TGGTGTTGAT GAATTCAAAG CATACTTTTC TGGCCTTGTG TGCTCTTCGT 
TTTATGAGGC GAATAATTGG CCTTAAGGAT GAGTTTTATA ATCGTTACAT CACCAAAGGA 
AATCTTTTTG AACCAGTTAT AAATGCCCTT TTGGATAATG GAACTCGGTA CAATCTATTA 
AATTCAGCTG TTATTGAATT GTTTGAATTT ATAAGAGTGG AAGATATCAA GTCTCTCACT 
GCACATATAG TTGAAAACTT TTATAAAGCA CTTGAATCGA TTGAATATGT TCAGACATTC 
AAAGGATTGA AGACTAAATA TGAACAAGAA AAAGACAGAC AAAATCAGAA ACTGAACAGT 
GTGCCATCTA TATTGCGTAG TAACCGATTT CGCAGAGATG CAAAAGCCTT GGAAGAGGAT 
GAAGAAATGT GGTTTAATGA AGATGATGAT GAAGAAGGAA AGTCAGTTGT AACACCAGTT 
GAAAAAACTA AGACTGAAGA TGATTTTCCA GATAGTTACG AAAAATTTAT GGAGACTAAA 
AAAGCAAAAG AAAGTGAAGA CAAGGAAAAC CTTCCCAAAA GGACATCTTC TGGTGGCTTC 
AAATTTACTT TCTCCCATTC CCCCAGTGCT GCTAATGGAA CAAACAGTAC AAACAGTAAG 
TCTGTAGTGG CTCAGACAAC ACCAGCAAGT TCTAATGGGT CCTCTTCCAA AACTACAAAC 
TTGGCTACAT CAGTGACAGC CACTAAGGGA AGTTTGGTTG GCTTAGTGGA CTATCCAGAT 
GATGAAGAGG AAGATGAGGA AGAAGAATCA TCCCCCAGAA AAAGACCTCG CCTTGGCTCA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Suppressor of MEK null (SMEK)/protein phosphatase 4 catalytic subunit (PP4C) is a key regulator of hepatic gluconeogenesis.
Proceedings of the National Academy of Sciences of the United States of America107(41)17704-9(2010 Oct)
Yoon YS,Lee MW,Ryu D,Kim JH,Ma H,Seo WY,Kim YN,Kim SS,Lee CH,Hunter T,Choi CS,Montminy MR,Koo SH

Identification of SMEK2 as a candidate gene for regulation of responsiveness to dietary cholesterol in rats.
Journal of lipid research50(1)41-6(2009 Jan)
Asahina M,Haruyama W,Ichida Y,Sakamoto M,Sato M,Imaizumi K

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Ppp4r3b cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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