gRNAs
The following Idua gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Idua cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
| CloneID | ORa06428 | |
| Clone ID Related Accession (Same CDS sequence) | NM_001172084.1 | |
| Accession Version | NM_001172084.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 1932bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector |  User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-09-09 | |
| Organism | Rattus norvegicus(Norway rat) | |
| Product | alpha-L-iduronidase precursor | |
| Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC117047.5. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END## | |
1 | ATGCGCCCCC CGCGCCCCGC CTCTGCTATG CTGATATTTT TTGCAGCGTT CTTGGCCTCG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_001165555.1 |
| CDS | 81..2012 |
| Translation |
Target ORF information:
Target ORF information:
|
 NM_001172084.1 |
1 | ATGCGCCCCC CGCGCCCCGC CTCTGCTATG CTGATATTTT TTGCAGCGTT CTTGGCCTCG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | ORa35806 | |
| Clone ID Related Accession (Same CDS sequence) | XM_008769938.2 | |
| Accession Version | XM_008769938.2 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 1650bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2016-07-25 | |
| Organism | Rattus norvegicus(Norway rat) | |
| Product | alpha-L-iduronidase isoform X1 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005113.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008769938.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGGAAGAAA GACTGAAGTC ATCAGGCCAG GGATTGATCT ACAACTTCAC CCACTTGGAT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_008768160.1 |
| CDS | 1043..2692 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_008769938.2 |
1 | ATGGAAGAAA GACTGAAGTC ATCAGGCCAG GGATTGATCT ACAACTTCAC CCACTTGGAT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
Identification and characterization of 20 novel pathogenic variants in 60 unrelated Indian patients with mucopolysaccharidoses type I and type II. |
Extracellular matrix disruption is an early event in the pathogenesis of skeletal disease in mucopolysaccharidosis I. |
?- L-iduronidase gene-based therapy using the phiC31 system to treat mucopolysaccharidose type I mice. |
High-dose enzyme replacement therapy in murine Hurler syndrome. |
Insights into mucopolysaccharidosis I from the structure and action of ?-L-iduronidase. |
