Naa25 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Naa25
Entrez Gene ID	360811
Full Name	N(alpha)-acetyltransferase 25, NatB auxiliary subunit
Synonyms	LRRGT00164,Mdm20,RGD1305685
General protein information	Preferred Names N(alpha)-acetyltransferase 25, NatB auxiliary subunit Names N-alpha-acetyltransferase 25, NatB auxiliary subunit N-terminal acetyltransferase B complex subunit MDM20 N-terminal acetyltransferase B complex subunit NAA25 liver regeneration-related protein LRRGT00164 mitochondrial distribution and morphology protein 20 natB complex subunit MDM20
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 12 Map Location: 12q16

mRNA and Protein(s)

mRNA	Protein	Name
NM_001047900.1	NP_001041365.1	N-alpha-acetyltransferase 25, NatB auxiliary subunit
XM_008769240.2	XP_008767462.1	N-alpha-acetyltransferase 25, NatB auxiliary subunit isoform X2
XM_006249394.3	XP_006249456.1	N-alpha-acetyltransferase 25, NatB auxiliary subunit isoform X1

Pathways from BioSystems

Homologies

	NAA25	XP_001102995.2
Bos taurus (cattle)	NAA25	NP_001193056.1
Danio rerio (zebrafish)	naa25	XP_005172656.1
Anopheles gambiae (African malaria mosquito)	NAA25_ANOGA	XP_321109.5
Caenorhabditis elegans (roundworm)	cra-1	NP_498494.2
Schizosaccharomyces pombe (fission yeast)	naa25	NP_595632.1
Xenopus tropicalis (tropical clawed frog)	naa25	XP_004910562.1
Pan troglodytes (chimpanzee)	NAA25	XP_509384.2
Canis lupus familiaris (dog)	NAA25	XP_543400.1
Drosophila melanogaster (fruit fly)	psidin	NP_650858.1
Homo sapiens (human)	NAA25	NP_079229.2
Mus musculus (house mouse)	Naa25	NP_766310.2
Rattus norvegicus (Norway rat)	Naa25	NP_001041365.1
Gallus gallus (chicken)	NAA25	NP_001025835.2

Gene Ontology
Bibliography

Related articles in PubMed

Mdm20 stimulates polyQ aggregation via inhibiting autophagy through Akt-Ser473 phosphorylation.
Yasuda K, Ohyama K, Onga K, Kakizuka A, Mori N
PloS one8(12)e82523(2013)

Spatio-temporal expression pattern of the NatB complex, Nat5/Mdm20 in the developing mouse brain: implications for co-operative versus non-co-operative actions of Mdm20 and Nat5.
Ohyama K, Yasuda K, Onga K, Kakizuka A, Mori N
Gene expression patterns : GEP12(1-2)36-45(2012 Jan-Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Mdm20, the so-called auxiliary subunit of the translation-coupled protein N-acetylation complex, contributes to protein clearance and/or aggregate formation by affecting the phosphorylation level of Akt indepenently from the function of Nat5.
Title: Mdm20 stimulates polyQ aggregation via inhibiting autophagy through Akt-Ser473 phosphorylation.

The following Naa25 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Naa25 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa41892	NM_001047900.1 Latest version!	Rattus norvegicus N(alpha)-acetyltransferase 25, NatB auxiliary subunit (Naa25), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$573.30 $819.00
ORa35058	XM_006249394.3 Latest version!	Rattus norvegicus N(alpha)-acetyltransferase 25, NatB auxiliary subunit (Naa25), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
ORa47760	XM_008769240.2 Latest version!	Rattus norvegicus N(alpha)-acetyltransferase 25, NatB auxiliary subunit (Naa25), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00

ORF Online Only Promotion

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Naa25 ( NM_001047900.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001041365.1 Rattus norvegicus N(alpha)-acetyltransferase 25, NatB auxiliary subunit (Naa25), mRNA.

Price & Availability↑

CloneID	ORa41892
Clone ID Related Accession (Same CDS sequence)	NM_001047900.1
Accession Version	NM_001047900.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2913bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Rattus norvegicus(Norway rat)
Product	N-alpha-acetyltransferase 25, NatB auxiliary subunit
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AY539915.1. On or before Sep 8, 2006 this sequence version replaced XM_341084.3, XM_001076242.1. ##Evidence-Data-START## Transcript exon combination :: AY539915.1 [ECO:0000332] ##Evidence-Data-END##

ATGGCGACGC GGGGCCATGT GCAGGACCCT AACGACCGGC GCCTCAGGCC CATTTACGAT 
TATCTTGATA ATGGAAATAA TAAAATGGCA ATTCAGCAAG CTGACAAACT GCTGAAGAAA 
CACAGAGATC TCCACTGTGC GAAGGTTCTC AAGGCGATTG GTTTACAGAG AACCGGAAGA 
CAGGAGGAAG CCTTCACCTT GGCTCAGGAG GTGGCTGCCC TTGAGCCCAC AGATGACAAT 
TCCCTGCAGG CCCTGACCAT CCTCTACCGA GAGATGCACC GGCCGGAGTT GGTCACAAAG 
CTATATGAGG CGGCTGTGAA GAAAGTCCCC AACAGCGAGG AGTATCACTC TCACCTCTTC 
ATGGCCTATG CCAGAGTGGG CGAGTACAAG AAGATGCAGC AGGCTGGCAT GGCTCTGTAT 
AAGATCGTCC CCAAAAACCC TTACTACTTT TGGTCCGTGA TGAGCTTAAT CATGCAATCT 
ATATCCGCAC GAGATGAGAA CCTCTCGAAA ACCATGTTTC TGCCCCTGGC AGAGAGGATG 
GTAGAAAAAA TGGTGAAGGA GGACAAGATA GAAGCTGAAG CTGAAGTTGA ACTTTATTAC 
ATGATCCTGG AACGCTTGGG AAAGTACCAA GAAGCCCTGG ATGTCATTAG AGGGAAATTA 
GGAGAAAAGT TGACAAGTGA AATTCAGAGT CGGGAAAACA AATGCATGGC CATGTACAAG 
AAACTGAGCA AGTGGCCGGA GTGCAACGCC CTTTCCCGAC GCCTCCTGTT GAAAAACTCA 
GATGACTGGC AGTTCTATCT GACTTATTTC GATTCTGTCT TTCGACTGAT TGAAGAAGCC 
TGGACGCCTC CTGCAGAAGG GGAGCACTCC TTAGAAGGGG AAGTACACTG CTCTGCAGAG 
GATGCTGTGA AGTTTATAGA GGACCGGATA ACAGAGGCAT CACAAAGCTC TCGCCATGTC 
CGGGGCCCAC ACCTAGCTAA GCTGGAGCTC ATCAGGCGCC TTCGGAGCCA AGGCTGCAAC 
GACGAGTTCA GACTGGGTGA CCCAGAAGAG TTAATGTTCC AGTATTTCAA GAAGTTCGGG 
GACAAGCCAT GTTGCTTCAC AGACCTGAAG GTGTTCGTTG ACCTCTTGCC TGCTGCCCAG 
TGCACACAAT TCATTAACCA GCTCCTCGGA GTTGTTCCAC TGTCGACGCC GACAGAGGAT 
AAGCTTGCCC TGCCGGCTGA CATCCGAGGC CTGCAGCAGC ATCTGTGTGT TGTGCAGCTG 
ACGAGGCTCC TCGGCTTGTA CCACAGCATG GATAAGAACC AGAAGCTGGA TGTGGTCAGA 
GAATTGATGT TACGGTACCA GCACGGACTG GAGTTTGGGC GCTCTTGCTT GAAGACAGAG 
TTGCAGTTCT CTGACTATTA CTGCCTCCTC GCTGTTCACG TGCTCATTGA CATATGGAGA 
GAAGCAGGTG AGGAGACTGC TGTGTGGCAG GCCCTGACTC TGCTGGAAGA AGGCTTAACC 
CACAGCCCAT CCAATGCTCA GTTCAAATTG CTGCTTGTTC GAATCTACTG CGTGCTGGGT 
GCATTTGAAC CCGTGGTGGA TCTTTACTCC AGCCTTGATG CCAAGCACAT CCAGCACGAC 
ACCATTGGCT ACCTTCTGAC CCGATACGCT GCATCCCTGG GTCAGTACGC TGCTGCCTCC 
CAGTCCTGTA ACTTCGCACT CAGGTTTTTC CACTCCAACC AGAAAGATAC CTCAGAATAC 
ATTATTCAAG CTTACAAATA TGGTGCATTT GAGAAGATCC CAGAGTTTAT CGCTTTTAGG 
AACAGGCTGA ATAATTCTCT CCACTTTGCA CAAGTGCGCA CTGAACGGAT GCTGTTAGAC 
CTTCTACTTG AAGCAAACAT ATCAACTAGC TTAGCAGAAA GCATCAAATC CATGAACCTG 
AGGCCAGAGG AAGATGATGT TCCATGGGAG GATTTGCGAG ACAACAGAGA CTTGGACGTT 
TTCTTCAGCT GGGATCCAAA AGACAGGAAT GTTTCTGAAG AGCATAAGAA GCTCTCCCTG 
GAGGAGGAGA CCATGTGGTT AAGAATTCGC TCCTTAACAT TGAGGCTGAT CAGCGCCCTT 
CCAAGTCTTA CCCACCCCGT GGAGCCAAGG AACTCAGAGA AGACGTCAGA GAACGGCGTG 
TCCTCTCGGG TCGACGTCCT CCGTTTGCTC CTCCAACAGC TAGAAGTGGC CGTGGAGACT 
GGGAAGCGGT TCATTGAGAA GGAAATTCAG TACCCCTTCC TTGGGCCGGT CCCCACCAGG 
ATGGGCAGGT TCTTCAGTTC TGGCTGCTGT CAGTGCCAGG TCCACTCCTT TCATCTTGTC 
AGTGATGTGT ACGAGCTCGA CACCAGTGGC TTAGAGGGTA CAGTGGATAT ACAGGAACGG 
ATAGAAAATA GTCTTGCATC TTTACTAGAA CTATTGAAAG GTGTCTTCAG CACATGTAAG 
GGTGACCTCT TAGAAGTTAA AGACGGCAAT GTGAAGACCC AGCCTGCAGT CCTAGAGAAT 
CTGGTCTTCT TTGTTGAGAC TATTTCTATC GTCCTTTGGG TGTCCAGCTA CTGTGAGAGT 
GTCTTGCGAC CATACAAATT AAATATTCAG AAAAAGAAAA AGAAGAAAAA AGAAACCAGC 
ATCGTCATGC CTCCGATTTT CACCAGTTTC CAGGACTATG TTACTGGGCT TCAAACTGTA 
ATTTCCAATG CTGTGGATCA TATTAAAGGA CTTGAAGCAC ATCTAATTGC ACTTAAACTT 
GAGGAGCTCA CGTTAGAGGA CACGTCTATC TCTCCGGTGA ATCTGAGTAA CTGTCATGTG 
CTGTCTGTTA GGCGCCGAGA CCCTGGGTCC TACTGGAAGC TGGGTGCTGT AGAGCCAATG 
TGTGCTGATG TGTGTCCGAG TGTGCAGATC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001041365.1
CDS	1..2913
Translation	MATRGHVQDPNDRRLRPIYDYLDNGNNKMAIQQADKLLKKHRDLHCAKVLKAIGLQRTGRQEEAFTLAQEVAALEPTDDNSLQALTILYREMHRPELVTKLYEAAVKKVPNSEEYHSHLFMAYARVGEYKKMQQAGMALYKIVPKNPYYFWSVMSLIMQSISARDENLSKTMFLPLAERMVEKMVKEDKIEAEAEVELYYMILERLGKYQEALDVIRGKLGEKLTSEIQSRENKCMAMYKKLSKWPECNALSRRLLLKNSDDWQFYLTYFDSVFRLIEEAWTPPAEGEHSLEGEVHCSAEDAVKFIEDRITEASQSSRHVRGPHLAKLELIRRLRSQGCNDEFRLGDPEELMFQYFKKFGDKPCCFTDLKVFVDLLPAAQCTQFINQLLGVVPLSTPTEDKLALPADIRGLQQHLCVVQLTRLLGLYHSMDKNQKLDVVRELMLRYQHGLEFGRSCLKTELQFSDYYCLLAVHVLIDIWREAGEETAVWQALTLLEEGLTHSPSNAQFKLLLVRIYCVLGAFEPVVDLYSSLDAKHIQHDTIGYLLTRYAASLGQYAAASQSCNFALRFFHSNQKDTSEYIIQAYKYGAFEKIPEFIAFRNRLNNSLHFAQVRTERMLLDLLLEANISTSLAESIKSMNLRPEEDDVPWEDLRDNRDLDVFFSWDPKDRNVSEEHKKLSLEEETMWLRIRSLTLRLISALPSLTHPVEPRNSEKTSENGVSSRVDVLRLLLQQLEVAVETGKRFIEKEIQYPFLGPVPTRMGRFFSSGCCQCQVHSFHLVSDVYELDTSGLEGTVDIQERIENSLASLLELLKGVFSTCKGDLLEVKDGNVKTQPAVLENLVFFVETISIVLWVSSYCESVLRPYKLNIQKKKKKKKETSIVMPPIFTSFQDYVTGLQTVISNAVDHIKGLEAHLIALKLEELTLEDTSISPVNLSNCHVLSVRRRDPGSYWKLGAVEPMCADVCPSVQI

Target ORF information:

RefSeq Version	NM_001047900.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus N(alpha)-acetyltransferase 25, NatB auxiliary subunit (Naa25), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001047900.1

ORF Insert Sequence:

ATGGCGACGC GGGGCCATGT GCAGGACCCT AACGACCGGC GCCTCAGGCC CATTTACGAT 
TATCTTGATA ATGGAAATAA TAAAATGGCA ATTCAGCAAG CTGACAAACT GCTGAAGAAA 
CACAGAGATC TCCACTGTGC GAAGGTTCTC AAGGCGATTG GTTTACAGAG AACCGGAAGA 
CAGGAGGAAG CCTTCACCTT GGCTCAGGAG GTGGCTGCCC TTGAGCCCAC AGATGACAAT 
TCCCTGCAGG CCCTGACCAT CCTCTACCGA GAGATGCACC GGCCGGAGTT GGTCACAAAG 
CTATATGAGG CGGCTGTGAA GAAAGTCCCC AACAGCGAGG AGTATCACTC TCACCTCTTC 
ATGGCCTATG CCAGAGTGGG CGAGTACAAG AAGATGCAGC AGGCTGGCAT GGCTCTGTAT 
AAGATCGTCC CCAAAAACCC TTACTACTTT TGGTCCGTGA TGAGCTTAAT CATGCAATCT 
ATATCCGCAC GAGATGAGAA CCTCTCGAAA ACCATGTTTC TGCCCCTGGC AGAGAGGATG 
GTAGAAAAAA TGGTGAAGGA GGACAAGATA GAAGCTGAAG CTGAAGTTGA ACTTTATTAC 
ATGATCCTGG AACGCTTGGG AAAGTACCAA GAAGCCCTGG ATGTCATTAG AGGGAAATTA 
GGAGAAAAGT TGACAAGTGA AATTCAGAGT CGGGAAAACA AATGCATGGC CATGTACAAG 
AAACTGAGCA AGTGGCCGGA GTGCAACGCC CTTTCCCGAC GCCTCCTGTT GAAAAACTCA 
GATGACTGGC AGTTCTATCT GACTTATTTC GATTCTGTCT TTCGACTGAT TGAAGAAGCC 
TGGACGCCTC CTGCAGAAGG GGAGCACTCC TTAGAAGGGG AAGTACACTG CTCTGCAGAG 
GATGCTGTGA AGTTTATAGA GGACCGGATA ACAGAGGCAT CACAAAGCTC TCGCCATGTC 
CGGGGCCCAC ACCTAGCTAA GCTGGAGCTC ATCAGGCGCC TTCGGAGCCA AGGCTGCAAC 
GACGAGTTCA GACTGGGTGA CCCAGAAGAG TTAATGTTCC AGTATTTCAA GAAGTTCGGG 
GACAAGCCAT GTTGCTTCAC AGACCTGAAG GTGTTCGTTG ACCTCTTGCC TGCTGCCCAG 
TGCACACAAT TCATTAACCA GCTCCTCGGA GTTGTTCCAC TGTCGACGCC GACAGAGGAT 
AAGCTTGCCC TGCCGGCTGA CATCCGAGGC CTGCAGCAGC ATCTGTGTGT TGTGCAGCTG 
ACGAGGCTCC TCGGCTTGTA CCACAGCATG GATAAGAACC AGAAGCTGGA TGTGGTCAGA 
GAATTGATGT TACGGTACCA GCACGGACTG GAGTTTGGGC GCTCTTGCTT GAAGACAGAG 
TTGCAGTTCT CTGACTATTA CTGCCTCCTC GCTGTTCACG TGCTCATTGA CATATGGAGA 
GAAGCAGGTG AGGAGACTGC TGTGTGGCAG GCCCTGACTC TGCTGGAAGA AGGCTTAACC 
CACAGCCCAT CCAATGCTCA GTTCAAATTG CTGCTTGTTC GAATCTACTG CGTGCTGGGT 
GCATTTGAAC CCGTGGTGGA TCTTTACTCC AGCCTTGATG CCAAGCACAT CCAGCACGAC 
ACCATTGGCT ACCTTCTGAC CCGATACGCT GCATCCCTGG GTCAGTACGC TGCTGCCTCC 
CAGTCCTGTA ACTTCGCACT CAGGTTTTTC CACTCCAACC AGAAAGATAC CTCAGAATAC 
ATTATTCAAG CTTACAAATA TGGTGCATTT GAGAAGATCC CAGAGTTTAT CGCTTTTAGG 
AACAGGCTGA ATAATTCTCT CCACTTTGCA CAAGTGCGCA CTGAACGGAT GCTGTTAGAC 
CTTCTACTTG AAGCAAACAT ATCAACTAGC TTAGCAGAAA GCATCAAATC CATGAACCTG 
AGGCCAGAGG AAGATGATGT TCCATGGGAG GATTTGCGAG ACAACAGAGA CTTGGACGTT 
TTCTTCAGCT GGGATCCAAA AGACAGGAAT GTTTCTGAAG AGCATAAGAA GCTCTCCCTG 
GAGGAGGAGA CCATGTGGTT AAGAATTCGC TCCTTAACAT TGAGGCTGAT CAGCGCCCTT 
CCAAGTCTTA CCCACCCCGT GGAGCCAAGG AACTCAGAGA AGACGTCAGA GAACGGCGTG 
TCCTCTCGGG TCGACGTCCT CCGTTTGCTC CTCCAACAGC TAGAAGTGGC CGTGGAGACT 
GGGAAGCGGT TCATTGAGAA GGAAATTCAG TACCCCTTCC TTGGGCCGGT CCCCACCAGG 
ATGGGCAGGT TCTTCAGTTC TGGCTGCTGT CAGTGCCAGG TCCACTCCTT TCATCTTGTC 
AGTGATGTGT ACGAGCTCGA CACCAGTGGC TTAGAGGGTA CAGTGGATAT ACAGGAACGG 
ATAGAAAATA GTCTTGCATC TTTACTAGAA CTATTGAAAG GTGTCTTCAG CACATGTAAG 
GGTGACCTCT TAGAAGTTAA AGACGGCAAT GTGAAGACCC AGCCTGCAGT CCTAGAGAAT 
CTGGTCTTCT TTGTTGAGAC TATTTCTATC GTCCTTTGGG TGTCCAGCTA CTGTGAGAGT 
GTCTTGCGAC CATACAAATT AAATATTCAG AAAAAGAAAA AGAAGAAAAA AGAAACCAGC 
ATCGTCATGC CTCCGATTTT CACCAGTTTC CAGGACTATG TTACTGGGCT TCAAACTGTA 
ATTTCCAATG CTGTGGATCA TATTAAAGGA CTTGAAGCAC ATCTAATTGC ACTTAAACTT 
GAGGAGCTCA CGTTAGAGGA CACGTCTATC TCTCCGGTGA ATCTGAGTAA CTGTCATGTG 
CTGTCTGTTA GGCGCCGAGA CCCTGGGTCC TACTGGAAGC TGGGTGCTGT AGAGCCAATG 
TGTGCTGATG TGTGTCCGAG TGTGCAGATC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Naa25 ( XM_006249394.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006249456.1 Rattus norvegicus N(alpha)-acetyltransferase 25, NatB auxiliary subunit (Naa25), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa35058
Clone ID Related Accession (Same CDS sequence)	XM_006249394.3
Accession Version	XM_006249394.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2919bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	N-alpha-acetyltransferase 25, NatB auxiliary subunit isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005111.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006249394.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGACGC GGGGCCATGT GCAGGACCCT AACGACCGGC GCCTCAGGCC CATTTACGAT 
TATCTTGATA ATGGAAATAA TAAAATGGCA ATTCAGCAAG CTGACAAACT GCTGAAGAAA 
CACAGAGATC TCCACTGTGC GAAGGTTCTC AAGGCGATTG GTTTACAGAG AACCGGAAGA 
CAGGAGGAAG CCTTCACCTT GGCTCAGGAG GTGGCTGCCC TTGAGCCCAC AGATGACAAT 
TCCCTGCAGG CCCTGACCAT CCTCTACCGA GAGATGCACC GGCCGGAGTT GGTCACAAAG 
CTATATGAGG CGGCTGTGAA GAAAGTCCCC AACAGCGAGG AGTATCACTC TCACCTCTTC 
ATGGCCTATG CCAGAGTGGG CGAGTACAAG AAGATGCAGC AGGCTGGCAT GGCTCTGTAT 
AAGATCGTCC CCAAAAACCC TTACTACTTT TGGTCCGTGA TGAGCTTAAT CATGCAATCT 
ATATCCGCAC GAGATGAGAA CCTCTCGAAA ACCATGTTTC TGCCCCTGGC AGAGAGGATG 
GTAGAAAAAA TGGTGAAGGA GGACAAGATA GAAGCTGAAG CTGAAGTTGA ACTTTATTAC 
ATGATCCTGG AACGCTTGGG AAAGTACCAA GAAGCCCTGG ATGTCATTAG AGGGAAATTA 
GGAGAAAAGT TGACAAGTGA AATTCAGAGT CGGGAAAACA AATGCATGGC CATGTACAAG 
AAACTGAGCA AGTGGCCGGA GTGCAACGCC CTTTCCCGAC GCCTCCTGTT GAAAAACTCA 
GATGACTGGC AGTTCTATCT GACTTATTTC GATTCTGTCT TTCGACTGAT TGAAGAAGCC 
TGGACGCCTC CTGCAGAAGG GGAGCACTCC TTAGAAGGGG AAGTACACTG CTCTGCAGAG 
GATGCTGTGA AGTTTATAGA GGACCGGATA ACAGAGGCAT CACAAAGCTC TCGCCATGTC 
CGGGGCCCAC ACCTAGCTAA GCTGGAGCTC ATCAGGCGCC TTCGGAGCCA AGGCTGCAAC 
GACGAGTTCA GACTGGGTGA CCCAGAAGAG TTAATGTTCC AGTATTTCAA GAAGTTCGGG 
GACAAGCCAT GTTGCTTCAC AGACCTGAAG GTGTTCGTTG ACCTCTTGCC TGCTGCCCAG 
TGCACACAAT TCATTAACCA GCTCCTCGGA GTTGTTCCAC TGTCGACGCC GACAGAGGAT 
AAGCTTGCCC TGCCGGCTGA CATCCGAGGC CTGCAGCAGC ATCTGTGTGT TGTGCAGCTG 
ACGAGGCTCC TCGGCTTGTA CCACAGCATG GATAAGAACC AGAAGCTGGA TGTGGTCAGA 
GAATTGATGT TACGGTACCA GCACGGACTG GAGTTTGGGC GCTCTTGCTT GAAGACAGAG 
TTGCAGTTCT CTGACTATTA CTGCCTCCTC GCTGTTCACG TGCTCATTGA CATATGGAGA 
GAAGCAGGTG AGGAGACTGC TGTGTGGCAG GCCCTGACTC TGCTGGAAGA AGGCTTAACC 
CACAGCCCAT CCAATGCTCA GTTCAAATTG CTGCTTGTTC GAATCTACTG CGTGCTGGGT 
GCATTTGAAC CCGTGGTGGA TCTTTACTCC AGCCTTGATG CCAAGCACAT CCAGCACGAC 
ACCATTGGCT ACCTTCTGAC CCGATACGCT GCATCCCTGG GTCAGTACGC TGCTGCCTCC 
CAGTCCTGTA ACTTCGCACT CAGGTTTTTC CACTCCAACC AGAAAGATAC CTCAGAATAC 
ATTATTCAAG CTTACAAATA TGGTGCATTT GAGAAGATCC CAGAGTTTAT CGCTTTTAGG 
AACAGGCTGA ATAATTCTCT CCACTTTGCA CAAGTGCGCA CTGAACGGAT GCTGTTAGAC 
CTTCTACTTG AAGCAAACAT ATCAACTAGC TTAGCAGAAA GCATCAAATC CATGAACCTG 
AGGCCAGAGG AAGATGATGT TCCATGGGAG GATTTGCGAG ACAACAGAGA CTTGGACGTT 
TTCTTCAGCT GGGATCCAAA AGACAGGAAT GTTTCTGAAG AGCATAAGAA GCTCTCCCTG 
GAGGAGGAGA CCATGTGGTT AAGAATTCGC TCCTTAACAT TGAGGCTGAT CAGCGCCCTT 
CCAAGTCTTA CCCACCCCGT GGAGCCAAGG AACTCAGAGA AGACGTCAGA GAACGGCGTG 
TCCTCTCGGG TCGACGTCCT CCGTTTGCTC CTCCAACAGC TAGAAGTGGC CGTGGAGACT 
GGGAAGCGGT TCATTGAGAA GGAAATTCAG TACCCCTTCC TTGGGCCGGT CCCCACCAGG 
ATGGGCAGGT TCTTCAGTTC TGGCTGCTGT CAGTGCCAGG TCCACTCCTT TCATCTTGTC 
AGTGATGTGT ACGAGCTCGA CACCAGTGGC TTAGAGGGTA CAGTGGATAT ACAGGAACGG 
ATAGAAAATA GTCTTGCATC TTTACTAGAA CTATTGAAAG GTGTCTTCAG CACATGTAAG 
GGTGACCTCT TAGAAGTTAA AGACGGCAAT GTGAAGACCC AGCCTGCAGT CCTAGAGAAT 
CTGGTCTTCT TTGTTGAGAC TATTTCTATC GTCCTTTGGG TGTCCAGCTA CTGTGAGAGT 
GTCTTGCGAC CATACAAATT AAATATTCAG AAAAAGAAAA AGAAGAAAAA AGAAACCAGC 
ATCGTCATGC CTCCGATTTT CACCAGTTTC CAGGACTATG TTACTGGGCT TCAAACTGTA 
ATTTCCAATG CTGTGGATCA TATTAAAGGA CTTGAAGCAC ATCTAATTGC ACTTAAACTT 
GAGGAGCTCA CGTTAGAGGA CACGTCTATC TCTCCGGAGG AAAGAAAGTT TTCAAGGACT 
GTGCAGGGCA AAGTGCAGAG CAGTTACCTG CACTCGCTTC TGGAGACGGG GGAGCTGCTG 
AGGAAAAGGC TTGAGACTAC AAAGAAACTA AAAATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006249456.1
CDS	48..2966
Translation	MATRGHVQDPNDRRLRPIYDYLDNGNNKMAIQQADKLLKKHRDLHCAKVLKAIGLQRTGRQEEAFTLAQEVAALEPTDDNSLQALTILYREMHRPELVTKLYEAAVKKVPNSEEYHSHLFMAYARVGEYKKMQQAGMALYKIVPKNPYYFWSVMSLIMQSISARDENLSKTMFLPLAERMVEKMVKEDKIEAEAEVELYYMILERLGKYQEALDVIRGKLGEKLTSEIQSRENKCMAMYKKLSKWPECNALSRRLLLKNSDDWQFYLTYFDSVFRLIEEAWTPPAEGEHSLEGEVHCSAEDAVKFIEDRITEASQSSRHVRGPHLAKLELIRRLRSQGCNDEFRLGDPEELMFQYFKKFGDKPCCFTDLKVFVDLLPAAQCTQFINQLLGVVPLSTPTEDKLALPADIRGLQQHLCVVQLTRLLGLYHSMDKNQKLDVVRELMLRYQHGLEFGRSCLKTELQFSDYYCLLAVHVLIDIWREAGEETAVWQALTLLEEGLTHSPSNAQFKLLLVRIYCVLGAFEPVVDLYSSLDAKHIQHDTIGYLLTRYAASLGQYAAASQSCNFALRFFHSNQKDTSEYIIQAYKYGAFEKIPEFIAFRNRLNNSLHFAQVRTERMLLDLLLEANISTSLAESIKSMNLRPEEDDVPWEDLRDNRDLDVFFSWDPKDRNVSEEHKKLSLEEETMWLRIRSLTLRLISALPSLTHPVEPRNSEKTSENGVSSRVDVLRLLLQQLEVAVETGKRFIEKEIQYPFLGPVPTRMGRFFSSGCCQCQVHSFHLVSDVYELDTSGLEGTVDIQERIENSLASLLELLKGVFSTCKGDLLEVKDGNVKTQPAVLENLVFFVETISIVLWVSSYCESVLRPYKLNIQKKKKKKKETSIVMPPIFTSFQDYVTGLQTVISNAVDHIKGLEAHLIALKLEELTLEDTSISPEERKFSRTVQGKVQSSYLHSLLETGELLRKRLETTKKLKI

Target ORF information:

RefSeq Version	XM_006249394.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus N(alpha)-acetyltransferase 25, NatB auxiliary subunit (Naa25), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006249394.3

ORF Insert Sequence:

ATGGCGACGC GGGGCCATGT GCAGGACCCT AACGACCGGC GCCTCAGGCC CATTTACGAT 
TATCTTGATA ATGGAAATAA TAAAATGGCA ATTCAGCAAG CTGACAAACT GCTGAAGAAA 
CACAGAGATC TCCACTGTGC GAAGGTTCTC AAGGCGATTG GTTTACAGAG AACCGGAAGA 
CAGGAGGAAG CCTTCACCTT GGCTCAGGAG GTGGCTGCCC TTGAGCCCAC AGATGACAAT 
TCCCTGCAGG CCCTGACCAT CCTCTACCGA GAGATGCACC GGCCGGAGTT GGTCACAAAG 
CTATATGAGG CGGCTGTGAA GAAAGTCCCC AACAGCGAGG AGTATCACTC TCACCTCTTC 
ATGGCCTATG CCAGAGTGGG CGAGTACAAG AAGATGCAGC AGGCTGGCAT GGCTCTGTAT 
AAGATCGTCC CCAAAAACCC TTACTACTTT TGGTCCGTGA TGAGCTTAAT CATGCAATCT 
ATATCCGCAC GAGATGAGAA CCTCTCGAAA ACCATGTTTC TGCCCCTGGC AGAGAGGATG 
GTAGAAAAAA TGGTGAAGGA GGACAAGATA GAAGCTGAAG CTGAAGTTGA ACTTTATTAC 
ATGATCCTGG AACGCTTGGG AAAGTACCAA GAAGCCCTGG ATGTCATTAG AGGGAAATTA 
GGAGAAAAGT TGACAAGTGA AATTCAGAGT CGGGAAAACA AATGCATGGC CATGTACAAG 
AAACTGAGCA AGTGGCCGGA GTGCAACGCC CTTTCCCGAC GCCTCCTGTT GAAAAACTCA 
GATGACTGGC AGTTCTATCT GACTTATTTC GATTCTGTCT TTCGACTGAT TGAAGAAGCC 
TGGACGCCTC CTGCAGAAGG GGAGCACTCC TTAGAAGGGG AAGTACACTG CTCTGCAGAG 
GATGCTGTGA AGTTTATAGA GGACCGGATA ACAGAGGCAT CACAAAGCTC TCGCCATGTC 
CGGGGCCCAC ACCTAGCTAA GCTGGAGCTC ATCAGGCGCC TTCGGAGCCA AGGCTGCAAC 
GACGAGTTCA GACTGGGTGA CCCAGAAGAG TTAATGTTCC AGTATTTCAA GAAGTTCGGG 
GACAAGCCAT GTTGCTTCAC AGACCTGAAG GTGTTCGTTG ACCTCTTGCC TGCTGCCCAG 
TGCACACAAT TCATTAACCA GCTCCTCGGA GTTGTTCCAC TGTCGACGCC GACAGAGGAT 
AAGCTTGCCC TGCCGGCTGA CATCCGAGGC CTGCAGCAGC ATCTGTGTGT TGTGCAGCTG 
ACGAGGCTCC TCGGCTTGTA CCACAGCATG GATAAGAACC AGAAGCTGGA TGTGGTCAGA 
GAATTGATGT TACGGTACCA GCACGGACTG GAGTTTGGGC GCTCTTGCTT GAAGACAGAG 
TTGCAGTTCT CTGACTATTA CTGCCTCCTC GCTGTTCACG TGCTCATTGA CATATGGAGA 
GAAGCAGGTG AGGAGACTGC TGTGTGGCAG GCCCTGACTC TGCTGGAAGA AGGCTTAACC 
CACAGCCCAT CCAATGCTCA GTTCAAATTG CTGCTTGTTC GAATCTACTG CGTGCTGGGT 
GCATTTGAAC CCGTGGTGGA TCTTTACTCC AGCCTTGATG CCAAGCACAT CCAGCACGAC 
ACCATTGGCT ACCTTCTGAC CCGATACGCT GCATCCCTGG GTCAGTACGC TGCTGCCTCC 
CAGTCCTGTA ACTTCGCACT CAGGTTTTTC CACTCCAACC AGAAAGATAC CTCAGAATAC 
ATTATTCAAG CTTACAAATA TGGTGCATTT GAGAAGATCC CAGAGTTTAT CGCTTTTAGG 
AACAGGCTGA ATAATTCTCT CCACTTTGCA CAAGTGCGCA CTGAACGGAT GCTGTTAGAC 
CTTCTACTTG AAGCAAACAT ATCAACTAGC TTAGCAGAAA GCATCAAATC CATGAACCTG 
AGGCCAGAGG AAGATGATGT TCCATGGGAG GATTTGCGAG ACAACAGAGA CTTGGACGTT 
TTCTTCAGCT GGGATCCAAA AGACAGGAAT GTTTCTGAAG AGCATAAGAA GCTCTCCCTG 
GAGGAGGAGA CCATGTGGTT AAGAATTCGC TCCTTAACAT TGAGGCTGAT CAGCGCCCTT 
CCAAGTCTTA CCCACCCCGT GGAGCCAAGG AACTCAGAGA AGACGTCAGA GAACGGCGTG 
TCCTCTCGGG TCGACGTCCT CCGTTTGCTC CTCCAACAGC TAGAAGTGGC CGTGGAGACT 
GGGAAGCGGT TCATTGAGAA GGAAATTCAG TACCCCTTCC TTGGGCCGGT CCCCACCAGG 
ATGGGCAGGT TCTTCAGTTC TGGCTGCTGT CAGTGCCAGG TCCACTCCTT TCATCTTGTC 
AGTGATGTGT ACGAGCTCGA CACCAGTGGC TTAGAGGGTA CAGTGGATAT ACAGGAACGG 
ATAGAAAATA GTCTTGCATC TTTACTAGAA CTATTGAAAG GTGTCTTCAG CACATGTAAG 
GGTGACCTCT TAGAAGTTAA AGACGGCAAT GTGAAGACCC AGCCTGCAGT CCTAGAGAAT 
CTGGTCTTCT TTGTTGAGAC TATTTCTATC GTCCTTTGGG TGTCCAGCTA CTGTGAGAGT 
GTCTTGCGAC CATACAAATT AAATATTCAG AAAAAGAAAA AGAAGAAAAA AGAAACCAGC 
ATCGTCATGC CTCCGATTTT CACCAGTTTC CAGGACTATG TTACTGGGCT TCAAACTGTA 
ATTTCCAATG CTGTGGATCA TATTAAAGGA CTTGAAGCAC ATCTAATTGC ACTTAAACTT 
GAGGAGCTCA CGTTAGAGGA CACGTCTATC TCTCCGGAGG AAAGAAAGTT TTCAAGGACT 
GTGCAGGGCA AAGTGCAGAG CAGTTACCTG CACTCGCTTC TGGAGACGGG GGAGCTGCTG 
AGGAAAAGGC TTGAGACTAC AAAGAAACTA AAAATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Naa25 ( XM_008769240.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008767462.1 Rattus norvegicus N(alpha)-acetyltransferase 25, NatB auxiliary subunit (Naa25), transcript variant X2, mRNA.

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CloneID	ORa47760
Clone ID Related Accession (Same CDS sequence)	XM_008769240.2
Accession Version	XM_008769240.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2718bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	N-alpha-acetyltransferase 25, NatB auxiliary subunit isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005111.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008769240.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGACGC GGGGCCATGT GCAGGACCCT AACGACCGGC GCCTCAGGCC CATTTACGAT 
TATCTTGATA ATGGAAATAA TAAAATGGCA ATTCAGCAAG CTGACAAACT GCTGAAGAAA 
CACAGAGATC TCCACTGTGC GAAGGTTCTC AAGGCGATTG GTTTACAGAG AACCGGAAGA 
CAGGAGGAAG CCTTCACCTT GGCTCAGGAG GTGGCTGCCC TTGAGCCCAC AGATGACAAT 
TCCCTGCAGG CCCTGACCAT CCTCTACCGA GAGATGCACC GGCCGGAGTT GGTCACAAAG 
CTATATGAGG CGGCTGTGAA GAAAGTCCCC AACAGCGAGG AGTATCACTC TCACCTCTTC 
ATGGCCTATG CCAGAGTGGG CGAGTACAAG AAGATGCAGC AGGCTGGCAT GGCTCTGTAT 
AAGATCGTCC CCAAAAACCC TTACTACTTT TGGTCCGTGA TGAGCTTAAT CATGCAATCT 
ATATCCGCAC GAGATGAGAA CCTCTCGAAA ACCATGTTTC TGCCCCTGGC AGAGAGGATG 
GTAGAAAAAA TGGTGAAGGA GGACAAGATA GAAGCTGAAG CTGAAGTTGA ACTTTATTAC 
ATGATCCTGG AACGCTTGGG AAAGTACCAA GAAGCCCTGG ATGTCATTAG AGGGAAATTA 
GGAGAAAAGT TGACAAGTGA AATTCAGAGT CGGGAAAACA AATGCATGGC CATGTACAAG 
AAACTGAGCA AGTGGCCGGA GTGCAACGCC CTTTCCCGAC GCCTCCTGTT GAAAAACTCA 
GATGACTGGC AGTTCTATCT GACTTATTTC GATTCTGTCT TTCGACTGAT TGAAGAAGCC 
TGGACGCCTC CTGCAGAAGG GGAGCACTCC TTAGAAGGGG AAGTACACTG CTCTGCAGAG 
GATGCTGTGA AGTTTATAGA GGACCGGATA ACAGAGGCAT CACAAAGCTC TCGCCATGTC 
CGGGGCCCAC ACCTAGCTAA GCTGGAGCTC ATCAGGCGCC TTCGGAGCCA AGGCTGCAAC 
GACGAGTTCA GACTGGGTGA CCCAGAAGAG TTAATGTTCC AGTATTTCAA GAAGTTCGGG 
GACAAGCCAT GTTGCTTCAC AGACCTGAAG GTGTTCGTTG ACCTCTTGCC TGCTGCCCAG 
TGCACACAAT TCATTAACCA GCTCCTCGGA GTTGTTCCAC TGTCGACGCC GACAGAGGAT 
AAGCTTGCCC TGCCGGCTGA CATCCGAGGC CTGCAGCAGC ATCTGTGTGT TGTGCAGCTG 
ACGAGGCTCC TCGGCTTGTA CCACAGCATG GATAAGAACC AGAAGCTGGA TGTGGTCAGA 
GAATTGATGT TACGGTACCA GCACGGACTG GAGTTTGGGC GCTCTTGCTT GAAGACAGAG 
TTGCAGTTCT CTGACTATTA CTGCCTCCTC GCTGTTCACG TGCTCATTGA CATATGGAGA 
GAAGCAGGTG AGGAGACTGC TGTGTGGCAG GCCCTGACTC TGCTGGAAGA AGGCTTAACC 
CACAGCCCAT CCAATGCTCA GTTCAAATTG CTGCTTGTTC GAATCTACTG CGTGCTGGGT 
GCATTTGAAC CCGTGGTGGA TCTTTACTCC AGCCTTGATG CCAAGCACAT CCAGCACGAC 
ACCATTGGCT ACCTTCTGAC CCGATACGCT GCATCCCTGG GTCAGTACGC TGCTGCCTCC 
CAGTCCTGTA ACTTCGCACT CAGGTTTTTC CACTCCAACC AGAAAGATAC CTCAGAATAC 
ATTATTCAAG CTTACAAATA TGGTGCATTT GAGAAGATCC CAGAGTTTAT CGCTTTTAGG 
AACAGGCTGA ATAATTCTCT CCACTTTGCA CAAGTGCGCA CTGAACGGAT GCTGTTAGAC 
CTTCTACTTG AAGCAAACAT ATCAACTAGC TTAGCAGAAA GCATCAAATC CATGAACCTG 
AGGCCAGAGG AAGATGATGT TCCATGGGAG GATTTGCGAG ACAACAGAGA CTTGGACGTT 
TTCTTCAGCT GGGATCCAAA AGACAGGAAT GTTTCTGAAG AGCATAAGAA GCTCTCCCTG 
GAGGAGGAGA CCATGTGGTT AAGAATTCGC TCCTTAACAT TGAGGCTGAT CAGCGCCCTT 
CCAAGTCTTA CCCACCCCGT GGAGCCAAGG AACTCAGAGA AGACGTCAGA GAACGGCGTG 
TCCTCTCGGG TCGACGTCCT CCGTTTGCTC CTCCAACAGC TAGAAGTGGC CGTGGAGACT 
GGGAAGCGGT TCATTGAGAA GGAAATTCAG TACCCCTTCC TTGGGCCGGT CCCCACCAGG 
ATGGGCAGGT TCTTCAGTTC TGGCTGCTGT CAGTGCCAGG TCCACTCCTT TCATCTTGTC 
AGTGATGTGT ACGAGCTCGA CACCAGTGGC TTAGAGGGTA CAGTGGATAT ACAGGAACGG 
ATAGAAAATA GTCTTGCATC TTTACTAGAA CTATTGAAAG GTGTCTTCAG CACATGTAAG 
GGTGACCTCT TAGAAGTTAA AGACGGCAAT GTGAAGACCC AGCCTGCAGT CCTAGAGAAT 
CTGGTCTTCT TTGTTGAGAC TATTTCTATC GTCCTTTGGG TGTCCAGCTA CTGTGAGAGT 
GTCTTGCGAC CATACAAATT AAATATTCAG AAAAAGAAAA AGAAGAAAAA AGAAACCAGC 
ATCGTCATGG TAATAACAAG GAACAGCCTC CGATTTTCAC CAGTTTCCAG GACTATGTTA 
CTGGGCTTCA AACTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008767462.1
CDS	48..2765
Translation	MATRGHVQDPNDRRLRPIYDYLDNGNNKMAIQQADKLLKKHRDLHCAKVLKAIGLQRTGRQEEAFTLAQEVAALEPTDDNSLQALTILYREMHRPELVTKLYEAAVKKVPNSEEYHSHLFMAYARVGEYKKMQQAGMALYKIVPKNPYYFWSVMSLIMQSISARDENLSKTMFLPLAERMVEKMVKEDKIEAEAEVELYYMILERLGKYQEALDVIRGKLGEKLTSEIQSRENKCMAMYKKLSKWPECNALSRRLLLKNSDDWQFYLTYFDSVFRLIEEAWTPPAEGEHSLEGEVHCSAEDAVKFIEDRITEASQSSRHVRGPHLAKLELIRRLRSQGCNDEFRLGDPEELMFQYFKKFGDKPCCFTDLKVFVDLLPAAQCTQFINQLLGVVPLSTPTEDKLALPADIRGLQQHLCVVQLTRLLGLYHSMDKNQKLDVVRELMLRYQHGLEFGRSCLKTELQFSDYYCLLAVHVLIDIWREAGEETAVWQALTLLEEGLTHSPSNAQFKLLLVRIYCVLGAFEPVVDLYSSLDAKHIQHDTIGYLLTRYAASLGQYAAASQSCNFALRFFHSNQKDTSEYIIQAYKYGAFEKIPEFIAFRNRLNNSLHFAQVRTERMLLDLLLEANISTSLAESIKSMNLRPEEDDVPWEDLRDNRDLDVFFSWDPKDRNVSEEHKKLSLEEETMWLRIRSLTLRLISALPSLTHPVEPRNSEKTSENGVSSRVDVLRLLLQQLEVAVETGKRFIEKEIQYPFLGPVPTRMGRFFSSGCCQCQVHSFHLVSDVYELDTSGLEGTVDIQERIENSLASLLELLKGVFSTCKGDLLEVKDGNVKTQPAVLENLVFFVETISIVLWVSSYCESVLRPYKLNIQKKKKKKKETSIVMVITRNSLRFSPVSRTMLLGFKL

Target ORF information:

RefSeq Version	XM_008769240.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus N(alpha)-acetyltransferase 25, NatB auxiliary subunit (Naa25), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008769240.2

ORF Insert Sequence:

ATGGCGACGC GGGGCCATGT GCAGGACCCT AACGACCGGC GCCTCAGGCC CATTTACGAT 
TATCTTGATA ATGGAAATAA TAAAATGGCA ATTCAGCAAG CTGACAAACT GCTGAAGAAA 
CACAGAGATC TCCACTGTGC GAAGGTTCTC AAGGCGATTG GTTTACAGAG AACCGGAAGA 
CAGGAGGAAG CCTTCACCTT GGCTCAGGAG GTGGCTGCCC TTGAGCCCAC AGATGACAAT 
TCCCTGCAGG CCCTGACCAT CCTCTACCGA GAGATGCACC GGCCGGAGTT GGTCACAAAG 
CTATATGAGG CGGCTGTGAA GAAAGTCCCC AACAGCGAGG AGTATCACTC TCACCTCTTC 
ATGGCCTATG CCAGAGTGGG CGAGTACAAG AAGATGCAGC AGGCTGGCAT GGCTCTGTAT 
AAGATCGTCC CCAAAAACCC TTACTACTTT TGGTCCGTGA TGAGCTTAAT CATGCAATCT 
ATATCCGCAC GAGATGAGAA CCTCTCGAAA ACCATGTTTC TGCCCCTGGC AGAGAGGATG 
GTAGAAAAAA TGGTGAAGGA GGACAAGATA GAAGCTGAAG CTGAAGTTGA ACTTTATTAC 
ATGATCCTGG AACGCTTGGG AAAGTACCAA GAAGCCCTGG ATGTCATTAG AGGGAAATTA 
GGAGAAAAGT TGACAAGTGA AATTCAGAGT CGGGAAAACA AATGCATGGC CATGTACAAG 
AAACTGAGCA AGTGGCCGGA GTGCAACGCC CTTTCCCGAC GCCTCCTGTT GAAAAACTCA 
GATGACTGGC AGTTCTATCT GACTTATTTC GATTCTGTCT TTCGACTGAT TGAAGAAGCC 
TGGACGCCTC CTGCAGAAGG GGAGCACTCC TTAGAAGGGG AAGTACACTG CTCTGCAGAG 
GATGCTGTGA AGTTTATAGA GGACCGGATA ACAGAGGCAT CACAAAGCTC TCGCCATGTC 
CGGGGCCCAC ACCTAGCTAA GCTGGAGCTC ATCAGGCGCC TTCGGAGCCA AGGCTGCAAC 
GACGAGTTCA GACTGGGTGA CCCAGAAGAG TTAATGTTCC AGTATTTCAA GAAGTTCGGG 
GACAAGCCAT GTTGCTTCAC AGACCTGAAG GTGTTCGTTG ACCTCTTGCC TGCTGCCCAG 
TGCACACAAT TCATTAACCA GCTCCTCGGA GTTGTTCCAC TGTCGACGCC GACAGAGGAT 
AAGCTTGCCC TGCCGGCTGA CATCCGAGGC CTGCAGCAGC ATCTGTGTGT TGTGCAGCTG 
ACGAGGCTCC TCGGCTTGTA CCACAGCATG GATAAGAACC AGAAGCTGGA TGTGGTCAGA 
GAATTGATGT TACGGTACCA GCACGGACTG GAGTTTGGGC GCTCTTGCTT GAAGACAGAG 
TTGCAGTTCT CTGACTATTA CTGCCTCCTC GCTGTTCACG TGCTCATTGA CATATGGAGA 
GAAGCAGGTG AGGAGACTGC TGTGTGGCAG GCCCTGACTC TGCTGGAAGA AGGCTTAACC 
CACAGCCCAT CCAATGCTCA GTTCAAATTG CTGCTTGTTC GAATCTACTG CGTGCTGGGT 
GCATTTGAAC CCGTGGTGGA TCTTTACTCC AGCCTTGATG CCAAGCACAT CCAGCACGAC 
ACCATTGGCT ACCTTCTGAC CCGATACGCT GCATCCCTGG GTCAGTACGC TGCTGCCTCC 
CAGTCCTGTA ACTTCGCACT CAGGTTTTTC CACTCCAACC AGAAAGATAC CTCAGAATAC 
ATTATTCAAG CTTACAAATA TGGTGCATTT GAGAAGATCC CAGAGTTTAT CGCTTTTAGG 
AACAGGCTGA ATAATTCTCT CCACTTTGCA CAAGTGCGCA CTGAACGGAT GCTGTTAGAC 
CTTCTACTTG AAGCAAACAT ATCAACTAGC TTAGCAGAAA GCATCAAATC CATGAACCTG 
AGGCCAGAGG AAGATGATGT TCCATGGGAG GATTTGCGAG ACAACAGAGA CTTGGACGTT 
TTCTTCAGCT GGGATCCAAA AGACAGGAAT GTTTCTGAAG AGCATAAGAA GCTCTCCCTG 
GAGGAGGAGA CCATGTGGTT AAGAATTCGC TCCTTAACAT TGAGGCTGAT CAGCGCCCTT 
CCAAGTCTTA CCCACCCCGT GGAGCCAAGG AACTCAGAGA AGACGTCAGA GAACGGCGTG 
TCCTCTCGGG TCGACGTCCT CCGTTTGCTC CTCCAACAGC TAGAAGTGGC CGTGGAGACT 
GGGAAGCGGT TCATTGAGAA GGAAATTCAG TACCCCTTCC TTGGGCCGGT CCCCACCAGG 
ATGGGCAGGT TCTTCAGTTC TGGCTGCTGT CAGTGCCAGG TCCACTCCTT TCATCTTGTC 
AGTGATGTGT ACGAGCTCGA CACCAGTGGC TTAGAGGGTA CAGTGGATAT ACAGGAACGG 
ATAGAAAATA GTCTTGCATC TTTACTAGAA CTATTGAAAG GTGTCTTCAG CACATGTAAG 
GGTGACCTCT TAGAAGTTAA AGACGGCAAT GTGAAGACCC AGCCTGCAGT CCTAGAGAAT 
CTGGTCTTCT TTGTTGAGAC TATTTCTATC GTCCTTTGGG TGTCCAGCTA CTGTGAGAGT 
GTCTTGCGAC CATACAAATT AAATATTCAG AAAAAGAAAA AGAAGAAAAA AGAAACCAGC 
ATCGTCATGG TAATAACAAG GAACAGCCTC CGATTTTCAC CAGTTTCCAG GACTATGTTA 
CTGGGCTTCA AACTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Mdm20 stimulates polyQ aggregation via inhibiting autophagy through Akt-Ser473 phosphorylation.
PloS one8(12)e82523(2013)
Yasuda K,Ohyama K,Onga K,Kakizuka A,Mori N

Spatio-temporal expression pattern of the NatB complex, Nat5/Mdm20 in the developing mouse brain: implications for co-operative versus non-co-operative actions of Mdm20 and Nat5.
Gene expression patterns : GEP12(1-2)36-45(2012 Jan-Feb)
Ohyama K,Yasuda K,Onga K,Kakizuka A,Mori N

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Naa25 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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