Ppp1r1b cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ppp1r1b
Entrez Gene ID	360616
Full Name	protein phosphatase 1, regulatory (inhibitor) subunit 1B
Synonyms	Darpp-32,Darpp32
General protein information	Preferred Names protein phosphatase 1, regulatory (inhibitor) subunit 1B Names protein phosphatase 1 regulatory subunit 1B dopamine- and cAMP-regulated neuronal phosphoprotein dopamine- and cAMP-regulated phosphoprotein DARPP-32 neuronal phosphoprotein DARPP-32
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 10 Map Location: 10q31
Summary	potent inhibitor of protein phosphatase 1 (PP1); may play a role in maintaining the levels of excitability of NMDA receptors [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_138521.1	NP_612530.1	protein phosphatase 1 regulatory subunit 1B
XM_006247344.3	XP_006247406.1	protein phosphatase 1 regulatory subunit 1B isoform X1

Pathways from BioSystems

Homologies

	PPP1R1B	XP_001089386.2
Homo sapiens (human)	PPP1R1B	NP_115568.2
Bos taurus (cattle)	PPP1R1B	NP_777072.1
Rattus norvegicus (Norway rat)	Ppp1r1b	NP_612530.1
Pan troglodytes (chimpanzee)	PPP1R1B	XP_001149814.2
Canis lupus familiaris (dog)	PPP1R1B	XP_005624616.1
Mus musculus (house mouse)	Ppp1r1b	NP_659077.1

Gene Ontology
Bibliography

Related articles in PubMed

Muscarinic acetylcholine M
Liu L, Huang Y, Huang Q, Zhao Z, Yu J, Wang L
Neuropharmacology117376-386(2017 May)

Intrastriatal injection of ionomycin profoundly changes motor response to l-DOPA and its underlying molecular mechanisms.
Han C, Nie S, Chen G, Ma K, Xiong N, Zhang Z, Xu Y, Wang T, Papa SM, Cao X
Neuroscience34023-33(2017 Jan)

Glutamate Counteracts Dopamine/PKA Signaling via Dephosphorylation of DARPP-32 Ser-97 and Alteration of Its Cytonuclear Distribution.
Nishi A, Matamales M, Musante V, Valjent E, Kuroiwa M, Kitahara Y, Rebholz H, Greengard P, Girault JA, Nairn AC
The Journal of biological chemistry292(4)1462-1476(2017 01)

PPAR? modulation of mesolimbic dopamine transmission rescues depression-related behaviors.
Scheggi S, Melis M, De Felice M, Aroni S, Muntoni AL, Pelliccia T, Gambarana C, De Montis MG, Pistis M
Neuropharmacology110(Pt A)251-259(2016 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

we also demonstrated frequent co-overexpression and positive correlation of DARPP-32, SRp20 and CD44E expression levels in human gastric primary tumors.
Title: Regulation of CD44E by DARPP-32-dependent activation of SRp20 splicing factor in gastric tumorigenesis.

In a model of subarachnoid hemorrhage, rats with hydrocephalus had more severe basal ganglia injury with greater DARPP-32 depletion than rats without hydrocephalus.
Title: Basal Ganglia Damage in Experimental Subarachnoid Hemorrhage.

Darpp32 interaction with beta-adducin may be the molecular pathway by which environmental changes may rapidly alter responsiveness of striatal neurons involved in the reward system.
Title: DARPP-32 interaction with adducin may mediate rapid environmental effects on striatal neurons.

Study demonstrated that DARPP-32 was not altered in prefrontal cortex and hippocampus in sepsis
Title: Evaluation of NCS-1, DARPP-32, and neurotrophins in hippocampus and prefrontal cortex in rats submitted to sepsis.

DARPP32 has a role in pluripotent stem cell differentiation toward medium-sized spiny neurons
Title: Developmentally coordinated extrinsic signals drive human pluripotent stem cell differentiation toward authentic DARPP-32+ medium-sized spiny neurons.

The following Ppp1r1b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ppp1r1b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa01552	NM_138521.1 Latest version!	Rattus norvegicus protein phosphatase 1, regulatory (inhibitor) subunit 1B (Ppp1r1b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00
ORa33710	XM_006247344.3 Latest version!	Rattus norvegicus protein phosphatase 1, regulatory (inhibitor) subunit 1B (Ppp1r1b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ppp1r1b ( NM_138521.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_612530.1 Rattus norvegicus protein phosphatase 1, regulatory (inhibitor) subunit 1B (Ppp1r1b), mRNA.

Price & Availability↑

CloneID	ORa01552
Clone ID Related Accession (Same CDS sequence)	NM_138521.1
Accession Version	NM_138521.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 618bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-10
Organism	Rattus norvegicus(Norway rat)
Product	protein phosphatase 1 regulatory subunit 1B
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AY601872.1. On Apr 19, 2005 this sequence version replaced XM_340891.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY601872.1, BC078954.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGACCCCA AGGACCGCAA GAAGATCCAG TTCTCTGTGC CCGCGCCCCC GAGCCAGCTC 
GACCCCCGAC AGGTGGAGAT GATCCGGCGC CGGAGGCCAA CCCCTGCCTT GCTGTTCCGG 
GTCTCAGAGC ATTCCTCGCC AGAGGAGGAG TCCTCTCCGC ACCAGAGAAC CTCAGGAGAG 
GGGCACCACC CAAAGTCGAA GAGACCCAAC CCCTGTGCCT ACACTCCCCC ATCACTGAAA 
GCTGTGCAAC GCATCGCTGA GTCCCACCTG CAGACCATCA GCAACCTGAG TGAGAACCAG 
GCCTCGGAGG AAGAGGACGA GTTAGGGGAG CTTCGGGAGC TGGGGTATCC ACAAGAGGAT 
GATGAAGAGG ATGAGGACGA GGATGAAGAG GAGGACGAAG AAGAAGACAG CCAGGCGGAG 
GTCCTGAAAG GCAGCAGGGG GACTGCGGGG CAGAAGCTTA CTTCTGGCCA GGGTCTGGAG 
GGGCCCTGGG AGCGCCCACC TCCTCTGGAT GAGCCCCAGA GAGATGGAAA CTCTGAGGAC 
CAAGGGGAAG GCAGAGCAAC ACAAAGTGAG CCGGGAGAGG AGCCTCGGCA CCCTACCCCC 
CCTGAGTCCG GCACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_612530.1
CDS	424..1041
Translation	MDPKDRKKIQFSVPAPPSQLDPRQVEMIRRRRPTPALLFRVSEHSSPEEESSPHQRTSGEGHHPKSKRPNPCAYTPPSLKAVQRIAESHLQTISNLSENQASEEEDELGELRELGYPQEDDEEDEDEDEEEDEEEDSQAEVLKGSRGTAGQKLTSGQGLEGPWERPPPLDEPQRDGNSEDQGEGRATQSEPGEEPRHPTPPESGT

Target ORF information:

RefSeq Version	NM_138521.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus protein phosphatase 1, regulatory (inhibitor) subunit 1B (Ppp1r1b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_138521.1

ORF Insert Sequence:

ATGGACCCCA AGGACCGCAA GAAGATCCAG TTCTCTGTGC CCGCGCCCCC GAGCCAGCTC 
GACCCCCGAC AGGTGGAGAT GATCCGGCGC CGGAGGCCAA CCCCTGCCTT GCTGTTCCGG 
GTCTCAGAGC ATTCCTCGCC AGAGGAGGAG TCCTCTCCGC ACCAGAGAAC CTCAGGAGAG 
GGGCACCACC CAAAGTCGAA GAGACCCAAC CCCTGTGCCT ACACTCCCCC ATCACTGAAA 
GCTGTGCAAC GCATCGCTGA GTCCCACCTG CAGACCATCA GCAACCTGAG TGAGAACCAG 
GCCTCGGAGG AAGAGGACGA GTTAGGGGAG CTTCGGGAGC TGGGGTATCC ACAAGAGGAT 
GATGAAGAGG ATGAGGACGA GGATGAAGAG GAGGACGAAG AAGAAGACAG CCAGGCGGAG 
GTCCTGAAAG GCAGCAGGGG GACTGCGGGG CAGAAGCTTA CTTCTGGCCA GGGTCTGGAG 
GGGCCCTGGG AGCGCCCACC TCCTCTGGAT GAGCCCCAGA GAGATGGAAA CTCTGAGGAC 
CAAGGGGAAG GCAGAGCAAC ACAAAGTGAG CCGGGAGAGG AGCCTCGGCA CCCTACCCCC 
CCTGAGTCCG GCACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ppp1r1b ( XM_006247344.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006247406.1 Rattus norvegicus protein phosphatase 1, regulatory (inhibitor) subunit 1B (Ppp1r1b), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa33710
Clone ID Related Accession (Same CDS sequence)	XM_006247344.3
Accession Version	XM_006247344.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 732bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	protein phosphatase 1 regulatory subunit 1B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005109.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006247344.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTACATT CCCCTGTCCC TCCAAGTCTT AACCCAATAA TGTTCCTTCA AACCTTGACA 
ATTCCCGAAC CCCCAGCCCA CAGCCCATCC CTTTTCCCTC CCCGCCTTTC GTCTTTGGGA 
AGCCGCCCAC ACTGTTCCTT TCCTCGGCTG CGTCTGAGCA GGTCTTCGTG GTGGTGGTGG 
TGGGGTGTTG CTGCTTTCTG GGTCACCGCA GAGGGAGCTG GGAGATTAGG GATATGGGTC 
AAGACTGTGA GGACTGCATG TGAACAGTCT ACTTGCCCAG CAAGGGCTTT ACCGGCTGCA 
GAGTGCTGTG ACCTCAGGCT CAGGACTGCT TCTGCTTTGG GAGCCCGGGG AAGAGCTGTG 
CAACGCATCG CTGAGTCCCA CCTGCAGACC ATCAGCAACC TGAGTGAGAA CCAGGCCTCG 
GAGGAAGAGG ACGAGTTAGG GGAGCTTCGG GAGCTGGGGT ATCCACAAGA GGATGATGAA 
GAGGATGAGG ACGAGGATGA AGAGGAGGAC GAAGAAGAAG ACAGCCAGGC GGAGGTCCTG 
AAAGGCAGCA GGGGGACTGC GGGGCAGAAG CTTACTTCTG GCCAGGGTCT GGAGGGGCCC 
TGGGAGCGCC CACCTCCTCT GGATGAGCCC CAGAGAGATG GAAACTCTGA GGACCAAGGG 
GAAGGCAGAG CAACACAAAG TGAGCCGGGA GAGGAGCCTC GGCACCCTAC CCCCCCTGAG 
TCCGGCACAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006247406.1
CDS	802..1533
Translation	MLHSPVPPSLNPIMFLQTLTIPEPPAHSPSLFPPRLSSLGSRPHCSFPRLRLSRSSWWWWWGVAAFWVTAEGAGRLGIWVKTVRTACEQSTCPARALPAAECCDLRLRTASALGARGRAVQRIAESHLQTISNLSENQASEEEDELGELRELGYPQEDDEEDEDEDEEEDEEEDSQAEVLKGSRGTAGQKLTSGQGLEGPWERPPPLDEPQRDGNSEDQGEGRATQSEPGEEPRHPTPPESGT

Target ORF information:

RefSeq Version	XM_006247344.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus protein phosphatase 1, regulatory (inhibitor) subunit 1B (Ppp1r1b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006247344.3

ORF Insert Sequence:

ATGCTACATT CCCCTGTCCC TCCAAGTCTT AACCCAATAA TGTTCCTTCA AACCTTGACA 
ATTCCCGAAC CCCCAGCCCA CAGCCCATCC CTTTTCCCTC CCCGCCTTTC GTCTTTGGGA 
AGCCGCCCAC ACTGTTCCTT TCCTCGGCTG CGTCTGAGCA GGTCTTCGTG GTGGTGGTGG 
TGGGGTGTTG CTGCTTTCTG GGTCACCGCA GAGGGAGCTG GGAGATTAGG GATATGGGTC 
AAGACTGTGA GGACTGCATG TGAACAGTCT ACTTGCCCAG CAAGGGCTTT ACCGGCTGCA 
GAGTGCTGTG ACCTCAGGCT CAGGACTGCT TCTGCTTTGG GAGCCCGGGG AAGAGCTGTG 
CAACGCATCG CTGAGTCCCA CCTGCAGACC ATCAGCAACC TGAGTGAGAA CCAGGCCTCG 
GAGGAAGAGG ACGAGTTAGG GGAGCTTCGG GAGCTGGGGT ATCCACAAGA GGATGATGAA 
GAGGATGAGG ACGAGGATGA AGAGGAGGAC GAAGAAGAAG ACAGCCAGGC GGAGGTCCTG 
AAAGGCAGCA GGGGGACTGC GGGGCAGAAG CTTACTTCTG GCCAGGGTCT GGAGGGGCCC 
TGGGAGCGCC CACCTCCTCT GGATGAGCCC CAGAGAGATG GAAACTCTGA GGACCAAGGG 
GAAGGCAGAG CAACACAAAG TGAGCCGGGA GAGGAGCCTC GGCACCCTAC CCCCCCTGAG 
TCCGGCACAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Muscarinic acetylcholine M
Neuropharmacology117376-386(2017 May)
Liu L,Huang Y,Huang Q,Zhao Z,Yu J,Wang L

Intrastriatal injection of ionomycin profoundly changes motor response to l-DOPA and its underlying molecular mechanisms.
Neuroscience34023-33(2017 Jan)
Han C,Nie S,Chen G,Ma K,Xiong N,Zhang Z,Xu Y,Wang T,Papa SM,Cao X

Glutamate Counteracts Dopamine/PKA Signaling via Dephosphorylation of DARPP-32 Ser-97 and Alteration of Its Cytonuclear Distribution.
The Journal of biological chemistry292(4)1462-1476(2017 01)
Nishi A,Matamales M,Musante V,Valjent E,Kuroiwa M,Kitahara Y,Rebholz H,Greengard P,Girault JA,Nairn AC

PPAR? modulation of mesolimbic dopamine transmission rescues depression-related behaviors.
Neuropharmacology110(Pt A)251-259(2016 Nov)
Scheggi S,Melis M,De Felice M,Aroni S,Muntoni AL,Pelliccia T,Gambarana C,De Montis MG,Pistis M

- Most Popular Services
  - 人工遺伝子合成& DNA合成-納期保証
- Services & Products

見積依頼

Eメール

japanmarket@genscript.com

電話

03-6811-6572

FAX

03-6811-6573
050-3174-7589(受注専用)

オンラインフォーム

オンライン注文

Ppp1r1b cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Most Popular Services

Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム

サポート

会社情報

サポート

会社情報