Myh4 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Myh4
Entrez Gene ID	360543
Full Name	myosin heavy chain 4
General protein information	Preferred Names myosin heavy chain 4 Names myosin-4 2B myosin heavy chain myHC-2b myh4 myosin heavy chain 2b myosin heavy chain type IIb myosin, heavy chain 4, skeletal muscle myosin, heavy polypeptide 4, skeletal muscle
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 10 Map Location: 10q24
Summary	fast myosin isoform expressed in skeletal muscle [RGD, Jun 2006].

mRNA and Protein(s)

mRNA	Protein	Name
NM_019325.1	NP_062198.1	myosin-4

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	MYH4	XP_523785.2
	MYH4	XP_002800337.1
Canis lupus familiaris (dog)	MYH4	NP_001070262.1
Danio rerio (zebrafish)	myhz2	NP_694514.2
Danio rerio (zebrafish)	myhz1.2	NP_001154918.1
Danio rerio (zebrafish)	myhb	XP_001339206.5
Xenopus tropicalis (tropical clawed frog)	myh3	NP_001006915.1
Homo sapiens (human)	MYH4	NP_060003.2
Rattus norvegicus (Norway rat)	Myh4	NP_062198.1
Danio rerio (zebrafish)	ns:zf-e68	XP_001924014.2
Danio rerio (zebrafish)	zgc:66156	XP_002666141.3
Bos taurus (cattle)	MYH4	XP_005220494.1
Mus musculus (house mouse)	Myh4	NP_034985.2
Danio rerio (zebrafish)	myhc4	NP_001018321.1
Danio rerio (zebrafish)	wu:fd14a01	XP_002667379.3
Danio rerio (zebrafish)	myhz1.1	NP_001108561.1
Danio rerio (zebrafish)	myha	XP_001924009.1
Xenopus tropicalis (tropical clawed frog)	LOC100492956	XP_002937039.1
Xenopus tropicalis (tropical clawed frog)	myh7	NP_001135564.1
Xenopus tropicalis (tropical clawed frog)	LOC100493121	XP_004919657.1

Gene Ontology
Bibliography

Related articles in PubMed

Heart failure-induced diaphragm myopathy.
Lima AR, Martinez PF, Damatto RL, Cezar MD, Guizoni DM, Bonomo C, Oliveira SA Jr, Dal-Pai Silva M, Zornoff LA, Okoshi K, Okoshi MP
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology34(2)333-45(2014)

Sternohyoid and diaphragm muscle form and function during postnatal development in the rat.
O'Connell RA, Carberry J, O'Halloran KD
Experimental physiology98(9)1386-400(2013 Sep)

Raftlin is involved in the nucleocapture complex to induce poly(I:C)-mediated TLR3 activation.
Watanabe A, Tatematsu M, Saeki K, Shibata S, Shime H, Yoshimura A, Obuse C, Seya T, Matsumoto M
The Journal of biological chemistry286(12)10702-11(2011 Mar)

Different adaptations of alpha-actinin isoforms to exercise training in rat skeletal muscles.
Ogura Y, Naito H, Kakigi R, Akema T, Sugiura T, Katamoto S, Aoki J
Acta physiologica (Oxford, England)196(3)341-9(2009 Jul)

Botulinum toxin-induced paralysis leads to slower myosin heavy chain isoform composition and reduced titin content in juvenile rat gastrocnemius muscle.
Legerlotz K, Matthews KG, McMahon CD, Smith HK
Muscle & nerve39(4)472-9(2009 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Exercise training showed a myosin heavy chain shift from type IIb to IIa skeletal muscles.
Title: Different adaptations of alpha-actinin isoforms to exercise training in rat skeletal muscles.

although the sarcomeric stability of paralyzed muscle might be impaired, moderate mechanical loading does not seem to affect paralyzed muscle protein composition
Title: Botulinum toxin-induced paralysis leads to slower myosin heavy chain isoform composition and reduced titin content in juvenile rat gastrocnemius muscle.

myosin IIB has a key role in promoting accumulation of mutant Ca(v)2.1Ct at the plasma membrane
Title: Toxicity and endocytosis of spinocerebellar ataxia type 6 polyglutamine domains: role of myosin IIb.

The following Myh4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Myh4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa04421	NM_019325.1 Latest version!	Rattus norvegicus myosin heavy chain 4 (Myh4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Myh4 ( NM_019325.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_062198.1 Rattus norvegicus myosin heavy chain 4 (Myh4), mRNA.

Price & Availability↑

CloneID	ORa04421
Clone ID Related Accession (Same CDS sequence)	NM_019325.1
Accession Version	NM_019325.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5820bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Rattus norvegicus(Norway rat)
Product	myosin-4
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC113948.1. On or before Jun 25, 2006 this sequence version replaced XM_340818.3, XM_001078843.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC113948.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2689600, SAMEA2689602 [ECO:0000348] ##Evidence-Data-END##

ATGAGTTCCG ACGCCGAGAT GGCCGTTTTT GGGGAGGCTG CTCCTTACCT CCGGAAGTCT 
GAAAAGGAGC GAATCGAGGC TCAGAACAAG CCTTTTGATG CCAAGTCATC AGTGTTTGTG 
GTGGATGCTA AGGAGTCCTA TGTGAAAGCA ACCGTGCAGA GCAGGGAAGG GGGGAAGGTG 
ACGGCCAAGA CCGAAGGTGG AGCTACTGTC ACAGTTAAAG AAGATCAAGT CTTCTCCATG 
AACCCTCCCA AGTACGACAA GATCGAGGAC ATGGCCATGA TGACCCACCT CCACGAGCCC 
GCTGTGCTGT ACAACCTCAA AGAGCGTTAT GCAGCCTGGA TGATCTACAC TTATTCGGGG 
CTCTTCTGTG TCACTGTCAA CCCCTACAAG TGGTTGCCGG TGTACAACCC CGAGGTGGTG 
GCAGCTTACC GAGGCAAAAA GCGACAGGAG GCCCCGCCCC ACATCTTCTC CATCTCTGAT 
AATGCCTACC AGTTCATGCT GACGGATCGT GAGAACCAGT CCATCCTGAT TACTGGAGAA 
TCCGGGGCCG GGAAGACTGT GAACACGAAG CGTGTCATCC AGTACTTTGC AACAATTGCA 
GTCACCGGGG ACAAGAAGAA GGAGGAAGCA CCTTCTGGCA AAATGCAGGG GACCCTTGAA 
GATCAAATCA TCAGTGCCAA CCCTCTGCTG GAGGCCTTTG GCAATGCCAA GACTGTGAGG 
AACGACAACT CCTCCCGCTT CGGTAAATTC ATCAGAATCC ATTTCGGTGC CACAGGCAAA 
CTGGCCTCCG CAGATATCGA AACCTATCTG CTAGAGAAGT CCAGAGTCAC CTTCCAGCTC 
AAGGCTGAAA GAAGCTATCA CATCTTCTAC CAAGTCATGT CCAATAAGAA ACCAGAGCTT 
ATCGAGATGC TCCTGATCAC CACCAACCCA TATGACTTTG CTTACGTCAG TCAAGGTGAA 
ATCACAGTGC CCAGCATTGA TGACCAGGAA GAGTTGATGG CCACAGACAC TGCCGTGGAC 
ATCCTGGGGT TCACTGCAGA TGAAAAAGTG GCCATTTACA AGCTCACTGG TGCTGTGATG 
CACTATGGGA ACATGAAATT CAAGCAAAAG CAAAGGGAAG AGCAGGCTGA GCCAGACGGC 
ACTGAAGTGG CTGACAAGGC TGCCTATCTG ACAAGTCTGA ACTCTGCTGA CCTGCTCAAA 
GCCCTCTGCT ACCCCAGGGT CAAGGTCGGC AATGAGTACG TCACCAAAGG CCAGACAGTG 
CAGCAGGTGT ACAACTCTGT GGGCGCCCTG GCCAAGGCCA TGTACGAGAA GATGTTCCTG 
TGGATGGTCA CCCGCATCAA CCAGCAGCTG GACACCAAGC AGCCCAGGCA GTACTTCATC 
GGGGTCTTGG ACATCGCTGG CTTTGAGATC TTTGACTTCA ACACCTTGGA GCAGCTGTGT 
ATCAACTTCA CCAACGAGAA ACTGCAACAG TTTTTCAACC ACCACATGTT CGTGCTGGAG 
CAGGAGGAGT ACAAGAAGGA AGGCATTGAG TGGGAGTTCA TCGACTTCGG GATGGACTTG 
GCAGCCTGCA TTGAGCTCAT TGAGAAGCCG ATGGGCATCT TCTCCATCCT GGAAGAGGAG 
TGCATGTTCC CCAAGGCGAC AGACACCTCC TTCAAGAACA AACTGTATGA GCAGCATCTT 
GGGAAGTCCA ACAACTTCCA GAAGCCCAAA CCTGCCAAAG GCAAGGCCGA GGCCCACTTC 
TCCCTGGTGC ACTATGCAGG CACCGTGGAC TACAACATTA TTGGGTGGCT GGACAAGAAC 
AAGGACCCCC TGAATGAGAC CGTGGTGGGG CTGTACCAGA AGTCCGGGTT GAAGACTCTG 
GCTTTCCTAT TTTCTGGGGG ACAAGCTGCT GAAGCAGAGG GTGGCGGCGG AAAGAAAGGT 
GGCAAGAAGA AGGGTTCTTC TTTCCAGACC GTGTCAGCTC TTTTCAGGGA GAATTTAAAT 
AAGCTGATGA CCAACTTGAA GAGCACCCAC CCCCACTTTG TCCGATGCCT CATTCCCAAT 
GAGACTAAGA CTCCTGGTGC CATGGAGCAC GAACTGGTCC TGCACCAGCT GAGGTGCAAC 
GGGGTGCTGG AAGGCATCCG CATCTGCAGG AAGGGGTTCC CCAGCAGGAT CCTCTACGCA 
GACTTCAAGC AGAGATACAA AGTTCTCAAT GCAAGTGCTA TCCCGGAAGG TCAGTTCATT 
GATAGCAAGA AGGCCTCTGA GAAGCTTCTA GGTTCCATTG ACATCGACCA TACCCAGTAC 
AAATTCGGTC ACACGAAGGT TTTCTTTAAA GCTGGCCTGC TGGGAACTCT AGAGGAGATG 
CGAGATGAAA AGCTGGCTCA GCTCATCACG CGCACTCAAG CCGTGTGCAG AGGGTACCTG 
ATGAGGGTGG AGTTCAGAAA GATGATGGAG AGGAGAGAGT CCATCTTCTG CATCCAGTAC 
AACGTCCGCG CCTTCATGAA CGTCAAGCAC TGGCCCTGGA TGAAGCTGTA TTTCAAGATC 
AAACCCCTGC TGAAGAGTGC AGAGACTGAG AAGGAGATGG CCACCATGAA GGAAGATTTT 
GAGAAGGCCA AGGAGGATCT GGCAAAGTCA GAGGCAAAAA GGAAAGAACT AGAAGAAAAG 
ATGGTGGCTC TGATGCAAGA GAAAAATGAT CTGCAGCTCC AGGTTCAAGC TGAAGCAGAT 
GGCTTAGCTG ATGCAGAGGA AAGATGCGAC CAGCTGATCA AAACTAAAAT CCAACTGGAG 
GCCAAAATCA AAGAGTTGAC TGAGAGGGCT GAGGATGAGG AAGAGATCAA TGCCGAGCTC 
ACCGCCAAGA AGAGGAAGCT GGAGGACGAG TGCTCAGAGC TGAAGAAAGA CATCGATGAC 
CTTGAGCTGA CACTGGCCAA GGTTGAGAAG GAGAAGCACG CCACGGAGAA CAAGGTGAAA 
AACCTCACAG AAGAGATGGC AGGCTTGGAT GAAAATATTG TTAAGCTGAC CAAGGAGAAG 
AAGGCCCTGC AGGAGGCCCA CCAGCAGACC CTGGATGACC TGCAGGCAGA GGAGGACAAA 
GTCAACACCC TGACCAAAGC CAAAACCAAG CTCGAACAGC AAGTGGATGA CCTTGAAGGA 
TCACTGGAAC AGGAAAAGAA GCTGAGGATG GACTTAGAAA GAGCCAAGAG GAAACTGGAG 
GGGGACCTGA AACTGGCCCA AGAGTCCACA ATGGACATAG AAAATGACAA ACAGCAGCTC 
GATGAGAAAC TCAAAAAGAA AGAGTTTGAA ATGAGCAACC TGCAGAGCAA GATTGAGGAT 
GAACAGGCCC TCGGCATGCA GCTGCAGAAG AAGATCAAAG AGTTGCAGGC CCGCATTGAG 
GAGCTGGAGG AGGAAATCGA GGCAGAGCGG GCCTCCAGGG CCAAAGCAGA GAAGCAGCGC 
TCTGACCTCT CCCGGGAACT GGAGGAGATC AGCGAGCGGC TGGAAGAAGC CGGCGGGGCC 
ACTTCAGCCC AGATCGAGAT GAACAAGAAG AGAGAGGCCG AGTTCCAGAA AATGCGCAGG 
GACTTGGAGG AGGCCACCCT GCAGCACGAA GCCACAGCAG CCGCTCTTCG GAAGAAGCAC 
GCGGACAGCG TGGCTGAGCT CGGGGAGCAG ATCGACAACC TGCAGCGGGT GAAGCAGAAG 
CTGGAGAAGG AGAAGAGTGA GCTGAAGATG GAGATCGACG ACCTCGCTAG CAACATGGAG 
ACTGTCTCCA AAGCCAAGGG GAACCTTGAG AAGATGTGCC GCACCCTGGA GGACCAACTG 
AGTGAAGTGA AAACCAAGGA GGAGGAGCAG CAGCGCCTGA TCAATGAGCT GTCAGCCCAG 
AAGGCACGCT TACACACAGA GTCAGGTGAG TTTTCCCGAC AGCTGGATGA GAAAGATGCT 
ATGGTTTCTC AGCTCTCCCG AGGCAAACAA GCGTTTACAC AACAGATTGA GGAGCTAAAG 
AGGCAGCTAG AAGAAGAGTC CAAGGCCAAG AACGCGCTGG CCCACGCCCT GCAGTCCGCT 
CGCCACGACT GTGACCTTCT GCGGGAGCAG TATGAGGAGG AGCAGGAAGC CAAGGCCGAG 
CTGCAGAGGG CCATGTCCAA GGCCAACAGC GAGGTGGCCC AGTGGAGGAC GAAATATGAG 
ACGGATGCCA TCCAGCGCAC GGAGGAGCTG GAGGAGGCCA AGAAGAAGCT GGCTCAGCGT 
CTGCAGGATG CTGAGGAGCA CGTAGAAGCT GTGAATTCCA AGTGTGCTTC TCTTGAAAAG 
ACGAAGCAGC GGCTGCAGAA TGAGGTGGAG GACCTCATGA TTGACGTGGA GAGGTCCAAC 
GCTGCCTGCG CTGCGCTTGA TAAGAAGCAG AGGAACTTTG ACAAGGTCCT GGCAGAATGG 
AAACAGAAGT ATGAGGAAAC TCAGGCTGAG CTCGAAGCCT CCCAGAAGGA GTCCCGTTCT 
CTCAGCACGG AGCTGTTCAA GGTGAAGAAC GCCTATGAGG AGTCTCTGGA TCAACTGGAG 
ACGCTAAAGA GAGAGAACAA GAATCTACAG CAGGAGATTT CTGACCTGAC CGAGCAGATC 
GCAGAGGGAG GGAAGCATAT CCACGAGCTG GAGAAAATCA AGAAGCAAAT TGACCAGGAA 
AAGAGTGAAT TACAGGCGTC TCTGGAGGAA GCGGAGGCCT CCCTTGAGCA TGAAGAAGGT 
AAAATCCTAC GCATCCAGCT GGAGTTGAAC CAGGTGAAAT CCGAGATTGA CCGCAAAATT 
GCTGAAAAAG ACGAAGAAAT CGATCAGCTG AAGAGAAACC ACCTCAGAGT AGTGGAGTCC 
ATGCAAAGCA CACTGGACGC TGAGATCCGC AGCAGAAACG ATGCTCTGAG GATTAAGAAG 
AAGATGGAGG GCGACCTCAA TGAAATGGAA ATCCAGTTGA ACCATGCCAA CCGCCAGGCT 
GCAGAGGCCA TCAGGAACCT TAGGAACACA CAGGGAATGC TCAAGGACAC GCAGCTGCAC 
CTGGACGATG CTCTCAGAGG CCAGGATGAC TTGAAAGAGC AGCTGGCCAT GGTTGAGCGC 
AGGGCAAACC TGATGCAGGC TGAGATCGAG GAACTCAGGG CATCCCTGGA ACAGACAGAG 
AGGAGCAGGA GAGTGGCTGA GCAAGAGCTA CTGGATGCCA GTGAGCGTGT GCAGCTCCTG 
CACACCCAGA ACACCAGCCT CATCAACACC AAGAAGAAGC TGGAGACAGA CATTTCCCAG 
ATCCAGGGAG AGATGGAGGA CATCGTCCAG GAAGCCCGCA ATGCAGAAGA GAAGGCCAAG 
AAAGCCATCA CTGACGCCGC CATGATGGCG GAGGAGCTGA AGAAGGAGCA GGACACCAGC 
GCCCACCTGG AGCGGATGAA GAAGAACATG GAGCAGACGG TGAAGGACCT GCAGCACCGT 
CTGGACGAGG CTGAGCAGCT GGCGCTGAAG GGCGGCAAGA AGCAGATCCA GAAACTGGAG 
GCCAGAGTGA GGGAGCTTGA AAACGAGGTG GAAAATGAAC AGAAGCGCAA CATTGAAGCT 
GTCAAGGGTC TTCGCAAGCA TGAGAGAAGA GTAAAGGAAC TCACCTACCA GACCGAGGAG 
GACCGCAAGA ACGTGCTGAG GCTGCAGGAC CTAGTGGATA AATTACAGAC TAAAGTGAAA 
GCCTACAAGA GACAAGCAGA GGAGGCTGAG GAACAATCCA ACGTCAACCT GGCCAAGTTC 
CGCAAGATCC AGCACGAGCT GGAGGAAGCC GAGGAGCGGG CCGACATCGC CGAGTCCCAG 
GTCAACAAGC TGCGGGTGAA GAGCCGAGAG GTTCACACCA AAGTCATAAG CGAAGAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_062198.1
CDS	96..5915
Translation	MSSDAEMAVFGEAAPYLRKSEKERIEAQNKPFDAKSSVFVVDAKESYVKATVQSREGGKVTAKTEGGATVTVKEDQVFSMNPPKYDKIEDMAMMTHLHEPAVLYNLKERYAAWMIYTYSGLFCVTVNPYKWLPVYNPEVVAAYRGKKRQEAPPHIFSISDNAYQFMLTDRENQSILITGESGAGKTVNTKRVIQYFATIAVTGDKKKEEAPSGKMQGTLEDQIISANPLLEAFGNAKTVRNDNSSRFGKFIRIHFGATGKLASADIETYLLEKSRVTFQLKAERSYHIFYQVMSNKKPELIEMLLITTNPYDFAYVSQGEITVPSIDDQEELMATDTAVDILGFTADEKVAIYKLTGAVMHYGNMKFKQKQREEQAEPDGTEVADKAAYLTSLNSADLLKALCYPRVKVGNEYVTKGQTVQQVYNSVGALAKAMYEKMFLWMVTRINQQLDTKQPRQYFIGVLDIAGFEIFDFNTLEQLCINFTNEKLQQFFNHHMFVLEQEEYKKEGIEWEFIDFGMDLAACIELIEKPMGIFSILEEECMFPKATDTSFKNKLYEQHLGKSNNFQKPKPAKGKAEAHFSLVHYAGTVDYNIIGWLDKNKDPLNETVVGLYQKSGLKTLAFLFSGGQAAEAEGGGGKKGGKKKGSSFQTVSALFRENLNKLMTNLKSTHPHFVRCLIPNETKTPGAMEHELVLHQLRCNGVLEGIRICRKGFPSRILYADFKQRYKVLNASAIPEGQFIDSKKASEKLLGSIDIDHTQYKFGHTKVFFKAGLLGTLEEMRDEKLAQLITRTQAVCRGYLMRVEFRKMMERRESIFCIQYNVRAFMNVKHWPWMKLYFKIKPLLKSAETEKEMATMKEDFEKAKEDLAKSEAKRKELEEKMVALMQEKNDLQLQVQAEADGLADAEERCDQLIKTKIQLEAKIKELTERAEDEEEINAELTAKKRKLEDECSELKKDIDDLELTLAKVEKEKHATENKVKNLTEEMAGLDENIVKLTKEKKALQEAHQQTLDDLQAEEDKVNTLTKAKTKLEQQVDDLEGSLEQEKKLRMDLERAKRKLEGDLKLAQESTMDIENDKQQLDEKLKKKEFEMSNLQSKIEDEQALGMQLQKKIKELQARIEELEEEIEAERASRAKAEKQRSDLSRELEEISERLEEAGGATSAQIEMNKKREAEFQKMRRDLEEATLQHEATAAALRKKHADSVAELGEQIDNLQRVKQKLEKEKSELKMEIDDLASNMETVSKAKGNLEKMCRTLEDQLSEVKTKEEEQQRLINELSAQKARLHTESGEFSRQLDEKDAMVSQLSRGKQAFTQQIEELKRQLEEESKAKNALAHALQSARHDCDLLREQYEEEQEAKAELQRAMSKANSEVAQWRTKYETDAIQRTEELEEAKKKLAQRLQDAEEHVEAVNSKCASLEKTKQRLQNEVEDLMIDVERSNAACAALDKKQRNFDKVLAEWKQKYEETQAELEASQKESRSLSTELFKVKNAYEESLDQLETLKRENKNLQQEISDLTEQIAEGGKHIHELEKIKKQIDQEKSELQASLEEAEASLEHEEGKILRIQLELNQVKSEIDRKIAEKDEEIDQLKRNHLRVVESMQSTLDAEIRSRNDALRIKKKMEGDLNEMEIQLNHANRQAAEAIRNLRNTQGMLKDTQLHLDDALRGQDDLKEQLAMVERRANLMQAEIEELRASLEQTERSRRVAEQELLDASERVQLLHTQNTSLINTKKKLETDISQIQGEMEDIVQEARNAEEKAKKAITDAAMMAEELKKEQDTSAHLERMKKNMEQTVKDLQHRLDEAEQLALKGGKKQIQKLEARVRELENEVENEQKRNIEAVKGLRKHERRVKELTYQTEEDRKNVLRLQDLVDKLQTKVKAYKRQAEEAEEQSNVNLAKFRKIQHELEEAEERADIAESQVNKLRVKSREVHTKVISEE

Target ORF information:

RefSeq Version	NM_019325.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus myosin heavy chain 4 (Myh4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_019325.1

ORF Insert Sequence:

ATGAGTTCCG ACGCCGAGAT GGCCGTTTTT GGGGAGGCTG CTCCTTACCT CCGGAAGTCT 
GAAAAGGAGC GAATCGAGGC TCAGAACAAG CCTTTTGATG CCAAGTCATC AGTGTTTGTG 
GTGGATGCTA AGGAGTCCTA TGTGAAAGCA ACCGTGCAGA GCAGGGAAGG GGGGAAGGTG 
ACGGCCAAGA CCGAAGGTGG AGCTACTGTC ACAGTTAAAG AAGATCAAGT CTTCTCCATG 
AACCCTCCCA AGTACGACAA GATCGAGGAC ATGGCCATGA TGACCCACCT CCACGAGCCC 
GCTGTGCTGT ACAACCTCAA AGAGCGTTAT GCAGCCTGGA TGATCTACAC TTATTCGGGG 
CTCTTCTGTG TCACTGTCAA CCCCTACAAG TGGTTGCCGG TGTACAACCC CGAGGTGGTG 
GCAGCTTACC GAGGCAAAAA GCGACAGGAG GCCCCGCCCC ACATCTTCTC CATCTCTGAT 
AATGCCTACC AGTTCATGCT GACGGATCGT GAGAACCAGT CCATCCTGAT TACTGGAGAA 
TCCGGGGCCG GGAAGACTGT GAACACGAAG CGTGTCATCC AGTACTTTGC AACAATTGCA 
GTCACCGGGG ACAAGAAGAA GGAGGAAGCA CCTTCTGGCA AAATGCAGGG GACCCTTGAA 
GATCAAATCA TCAGTGCCAA CCCTCTGCTG GAGGCCTTTG GCAATGCCAA GACTGTGAGG 
AACGACAACT CCTCCCGCTT CGGTAAATTC ATCAGAATCC ATTTCGGTGC CACAGGCAAA 
CTGGCCTCCG CAGATATCGA AACCTATCTG CTAGAGAAGT CCAGAGTCAC CTTCCAGCTC 
AAGGCTGAAA GAAGCTATCA CATCTTCTAC CAAGTCATGT CCAATAAGAA ACCAGAGCTT 
ATCGAGATGC TCCTGATCAC CACCAACCCA TATGACTTTG CTTACGTCAG TCAAGGTGAA 
ATCACAGTGC CCAGCATTGA TGACCAGGAA GAGTTGATGG CCACAGACAC TGCCGTGGAC 
ATCCTGGGGT TCACTGCAGA TGAAAAAGTG GCCATTTACA AGCTCACTGG TGCTGTGATG 
CACTATGGGA ACATGAAATT CAAGCAAAAG CAAAGGGAAG AGCAGGCTGA GCCAGACGGC 
ACTGAAGTGG CTGACAAGGC TGCCTATCTG ACAAGTCTGA ACTCTGCTGA CCTGCTCAAA 
GCCCTCTGCT ACCCCAGGGT CAAGGTCGGC AATGAGTACG TCACCAAAGG CCAGACAGTG 
CAGCAGGTGT ACAACTCTGT GGGCGCCCTG GCCAAGGCCA TGTACGAGAA GATGTTCCTG 
TGGATGGTCA CCCGCATCAA CCAGCAGCTG GACACCAAGC AGCCCAGGCA GTACTTCATC 
GGGGTCTTGG ACATCGCTGG CTTTGAGATC TTTGACTTCA ACACCTTGGA GCAGCTGTGT 
ATCAACTTCA CCAACGAGAA ACTGCAACAG TTTTTCAACC ACCACATGTT CGTGCTGGAG 
CAGGAGGAGT ACAAGAAGGA AGGCATTGAG TGGGAGTTCA TCGACTTCGG GATGGACTTG 
GCAGCCTGCA TTGAGCTCAT TGAGAAGCCG ATGGGCATCT TCTCCATCCT GGAAGAGGAG 
TGCATGTTCC CCAAGGCGAC AGACACCTCC TTCAAGAACA AACTGTATGA GCAGCATCTT 
GGGAAGTCCA ACAACTTCCA GAAGCCCAAA CCTGCCAAAG GCAAGGCCGA GGCCCACTTC 
TCCCTGGTGC ACTATGCAGG CACCGTGGAC TACAACATTA TTGGGTGGCT GGACAAGAAC 
AAGGACCCCC TGAATGAGAC CGTGGTGGGG CTGTACCAGA AGTCCGGGTT GAAGACTCTG 
GCTTTCCTAT TTTCTGGGGG ACAAGCTGCT GAAGCAGAGG GTGGCGGCGG AAAGAAAGGT 
GGCAAGAAGA AGGGTTCTTC TTTCCAGACC GTGTCAGCTC TTTTCAGGGA GAATTTAAAT 
AAGCTGATGA CCAACTTGAA GAGCACCCAC CCCCACTTTG TCCGATGCCT CATTCCCAAT 
GAGACTAAGA CTCCTGGTGC CATGGAGCAC GAACTGGTCC TGCACCAGCT GAGGTGCAAC 
GGGGTGCTGG AAGGCATCCG CATCTGCAGG AAGGGGTTCC CCAGCAGGAT CCTCTACGCA 
GACTTCAAGC AGAGATACAA AGTTCTCAAT GCAAGTGCTA TCCCGGAAGG TCAGTTCATT 
GATAGCAAGA AGGCCTCTGA GAAGCTTCTA GGTTCCATTG ACATCGACCA TACCCAGTAC 
AAATTCGGTC ACACGAAGGT TTTCTTTAAA GCTGGCCTGC TGGGAACTCT AGAGGAGATG 
CGAGATGAAA AGCTGGCTCA GCTCATCACG CGCACTCAAG CCGTGTGCAG AGGGTACCTG 
ATGAGGGTGG AGTTCAGAAA GATGATGGAG AGGAGAGAGT CCATCTTCTG CATCCAGTAC 
AACGTCCGCG CCTTCATGAA CGTCAAGCAC TGGCCCTGGA TGAAGCTGTA TTTCAAGATC 
AAACCCCTGC TGAAGAGTGC AGAGACTGAG AAGGAGATGG CCACCATGAA GGAAGATTTT 
GAGAAGGCCA AGGAGGATCT GGCAAAGTCA GAGGCAAAAA GGAAAGAACT AGAAGAAAAG 
ATGGTGGCTC TGATGCAAGA GAAAAATGAT CTGCAGCTCC AGGTTCAAGC TGAAGCAGAT 
GGCTTAGCTG ATGCAGAGGA AAGATGCGAC CAGCTGATCA AAACTAAAAT CCAACTGGAG 
GCCAAAATCA AAGAGTTGAC TGAGAGGGCT GAGGATGAGG AAGAGATCAA TGCCGAGCTC 
ACCGCCAAGA AGAGGAAGCT GGAGGACGAG TGCTCAGAGC TGAAGAAAGA CATCGATGAC 
CTTGAGCTGA CACTGGCCAA GGTTGAGAAG GAGAAGCACG CCACGGAGAA CAAGGTGAAA 
AACCTCACAG AAGAGATGGC AGGCTTGGAT GAAAATATTG TTAAGCTGAC CAAGGAGAAG 
AAGGCCCTGC AGGAGGCCCA CCAGCAGACC CTGGATGACC TGCAGGCAGA GGAGGACAAA 
GTCAACACCC TGACCAAAGC CAAAACCAAG CTCGAACAGC AAGTGGATGA CCTTGAAGGA 
TCACTGGAAC AGGAAAAGAA GCTGAGGATG GACTTAGAAA GAGCCAAGAG GAAACTGGAG 
GGGGACCTGA AACTGGCCCA AGAGTCCACA ATGGACATAG AAAATGACAA ACAGCAGCTC 
GATGAGAAAC TCAAAAAGAA AGAGTTTGAA ATGAGCAACC TGCAGAGCAA GATTGAGGAT 
GAACAGGCCC TCGGCATGCA GCTGCAGAAG AAGATCAAAG AGTTGCAGGC CCGCATTGAG 
GAGCTGGAGG AGGAAATCGA GGCAGAGCGG GCCTCCAGGG CCAAAGCAGA GAAGCAGCGC 
TCTGACCTCT CCCGGGAACT GGAGGAGATC AGCGAGCGGC TGGAAGAAGC CGGCGGGGCC 
ACTTCAGCCC AGATCGAGAT GAACAAGAAG AGAGAGGCCG AGTTCCAGAA AATGCGCAGG 
GACTTGGAGG AGGCCACCCT GCAGCACGAA GCCACAGCAG CCGCTCTTCG GAAGAAGCAC 
GCGGACAGCG TGGCTGAGCT CGGGGAGCAG ATCGACAACC TGCAGCGGGT GAAGCAGAAG 
CTGGAGAAGG AGAAGAGTGA GCTGAAGATG GAGATCGACG ACCTCGCTAG CAACATGGAG 
ACTGTCTCCA AAGCCAAGGG GAACCTTGAG AAGATGTGCC GCACCCTGGA GGACCAACTG 
AGTGAAGTGA AAACCAAGGA GGAGGAGCAG CAGCGCCTGA TCAATGAGCT GTCAGCCCAG 
AAGGCACGCT TACACACAGA GTCAGGTGAG TTTTCCCGAC AGCTGGATGA GAAAGATGCT 
ATGGTTTCTC AGCTCTCCCG AGGCAAACAA GCGTTTACAC AACAGATTGA GGAGCTAAAG 
AGGCAGCTAG AAGAAGAGTC CAAGGCCAAG AACGCGCTGG CCCACGCCCT GCAGTCCGCT 
CGCCACGACT GTGACCTTCT GCGGGAGCAG TATGAGGAGG AGCAGGAAGC CAAGGCCGAG 
CTGCAGAGGG CCATGTCCAA GGCCAACAGC GAGGTGGCCC AGTGGAGGAC GAAATATGAG 
ACGGATGCCA TCCAGCGCAC GGAGGAGCTG GAGGAGGCCA AGAAGAAGCT GGCTCAGCGT 
CTGCAGGATG CTGAGGAGCA CGTAGAAGCT GTGAATTCCA AGTGTGCTTC TCTTGAAAAG 
ACGAAGCAGC GGCTGCAGAA TGAGGTGGAG GACCTCATGA TTGACGTGGA GAGGTCCAAC 
GCTGCCTGCG CTGCGCTTGA TAAGAAGCAG AGGAACTTTG ACAAGGTCCT GGCAGAATGG 
AAACAGAAGT ATGAGGAAAC TCAGGCTGAG CTCGAAGCCT CCCAGAAGGA GTCCCGTTCT 
CTCAGCACGG AGCTGTTCAA GGTGAAGAAC GCCTATGAGG AGTCTCTGGA TCAACTGGAG 
ACGCTAAAGA GAGAGAACAA GAATCTACAG CAGGAGATTT CTGACCTGAC CGAGCAGATC 
GCAGAGGGAG GGAAGCATAT CCACGAGCTG GAGAAAATCA AGAAGCAAAT TGACCAGGAA 
AAGAGTGAAT TACAGGCGTC TCTGGAGGAA GCGGAGGCCT CCCTTGAGCA TGAAGAAGGT 
AAAATCCTAC GCATCCAGCT GGAGTTGAAC CAGGTGAAAT CCGAGATTGA CCGCAAAATT 
GCTGAAAAAG ACGAAGAAAT CGATCAGCTG AAGAGAAACC ACCTCAGAGT AGTGGAGTCC 
ATGCAAAGCA CACTGGACGC TGAGATCCGC AGCAGAAACG ATGCTCTGAG GATTAAGAAG 
AAGATGGAGG GCGACCTCAA TGAAATGGAA ATCCAGTTGA ACCATGCCAA CCGCCAGGCT 
GCAGAGGCCA TCAGGAACCT TAGGAACACA CAGGGAATGC TCAAGGACAC GCAGCTGCAC 
CTGGACGATG CTCTCAGAGG CCAGGATGAC TTGAAAGAGC AGCTGGCCAT GGTTGAGCGC 
AGGGCAAACC TGATGCAGGC TGAGATCGAG GAACTCAGGG CATCCCTGGA ACAGACAGAG 
AGGAGCAGGA GAGTGGCTGA GCAAGAGCTA CTGGATGCCA GTGAGCGTGT GCAGCTCCTG 
CACACCCAGA ACACCAGCCT CATCAACACC AAGAAGAAGC TGGAGACAGA CATTTCCCAG 
ATCCAGGGAG AGATGGAGGA CATCGTCCAG GAAGCCCGCA ATGCAGAAGA GAAGGCCAAG 
AAAGCCATCA CTGACGCCGC CATGATGGCG GAGGAGCTGA AGAAGGAGCA GGACACCAGC 
GCCCACCTGG AGCGGATGAA GAAGAACATG GAGCAGACGG TGAAGGACCT GCAGCACCGT 
CTGGACGAGG CTGAGCAGCT GGCGCTGAAG GGCGGCAAGA AGCAGATCCA GAAACTGGAG 
GCCAGAGTGA GGGAGCTTGA AAACGAGGTG GAAAATGAAC AGAAGCGCAA CATTGAAGCT 
GTCAAGGGTC TTCGCAAGCA TGAGAGAAGA GTAAAGGAAC TCACCTACCA GACCGAGGAG 
GACCGCAAGA ACGTGCTGAG GCTGCAGGAC CTAGTGGATA AATTACAGAC TAAAGTGAAA 
GCCTACAAGA GACAAGCAGA GGAGGCTGAG GAACAATCCA ACGTCAACCT GGCCAAGTTC 
CGCAAGATCC AGCACGAGCT GGAGGAAGCC GAGGAGCGGG CCGACATCGC CGAGTCCCAG 
GTCAACAAGC TGCGGGTGAA GAGCCGAGAG GTTCACACCA AAGTCATAAG CGAAGAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Heart failure-induced diaphragm myopathy.
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology34(2)333-45(2014)
Lima AR,Martinez PF,Damatto RL,Cezar MD,Guizoni DM,Bonomo C,Oliveira SA Jr,Dal-Pai Silva M,Zornoff LA,Okoshi K,Okoshi MP

Sternohyoid and diaphragm muscle form and function during postnatal development in the rat.
Experimental physiology98(9)1386-400(2013 Sep)
O'Connell RA,Carberry J,O'Halloran KD

Raftlin is involved in the nucleocapture complex to induce poly(I:C)-mediated TLR3 activation.
The Journal of biological chemistry286(12)10702-11(2011 Mar)
Watanabe A,Tatematsu M,Saeki K,Shibata S,Shime H,Yoshimura A,Obuse C,Seya T,Matsumoto M

Different adaptations of alpha-actinin isoforms to exercise training in rat skeletal muscles.
Acta physiologica (Oxford, England)196(3)341-9(2009 Jul)
Ogura Y,Naito H,Kakigi R,Akema T,Sugiura T,Katamoto S,Aoki J

Botulinum toxin-induced paralysis leads to slower myosin heavy chain isoform composition and reduced titin content in juvenile rat gastrocnemius muscle.
Muscle & nerve39(4)472-9(2009 Apr)
Legerlotz K,Matthews KG,McMahon CD,Smith HK

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Myh4 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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