Hsd3b1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Hsd3b1
Entrez Gene ID	360348
Full Name	hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 1
General protein information	Preferred Names hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 1 Names 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1 3 beta-hydroxysteroid dehydrogenase 1 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type I 3-beta-HSD I 3betaHSD hydroxysteroid dehydrogenase-1, delta<5>-3-beta
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 2 Map Location: 2q34

mRNA and Protein(s)

mRNA	Protein	Name
NM_001007719.3	NP_001007720.3	3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Different levels of leptin regulate different target enzymes involved in progesterone synthesis.
Bilbao MG, Di Yorio MP, Faletti AG
Fertility and sterility99(5)1460-6(2013 Apr)

Pharmacological doses of testosterone upregulated androgen receptor and 3-Beta-hydroxysteroid dehydrogenase/delta-5-delta-4 isomerase and impaired leydig cells steroidogenesis in adult rats.
Kostic TS, Stojkov NJ, Bjelic MM, Mihajlovic AI, Janjic MM, Andric SA
Toxicological sciences : an official journal of the Society of Toxicology121(2)397-407(2011 Jun)

Semicomprehensive analysis of the postnatal age-related changes in the mRNA expression of sex steroidogenic enzymes and sex steroid receptors in the male rat hippocampus.
Kimoto T, Ishii H, Higo S, Hojo Y, Kawato S
Endocrinology151(12)5795-806(2010 Dec)

[Effect of L-tyrosine on 3beta-HSD activity of rat luteal cells in vitro].
Wang HP, Ni J, Cheng ZP
Zhongguo ying yong sheng li xue za zhi = Zhongguo yingyong shenglixue zazhi = Chinese journal of applied physiology20(2)190-3(2004 May)

Regulation of expression of 3?-hydroxysteroid dehydrogenase is mediated by cAMP in rat Leydig cells and H540 rat Leydig tumor cells.
Keeney DS, Mason JI
The Journal of steroid biochemistry and molecular biology43(8)915-22(1992 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Leptin, which inhibits the ovulatory process, caused a significant reduction in the ovarian expression of P450scc without changes in StAR or 3betaHSD.
Title: Different levels of leptin regulate different target enzymes involved in progesterone synthesis.

The upregulation of AR and 3betaHSD in testosterone-impaired Leydig cells steroidogenesis could be the possible mechanism that maintains and prevents loss of steroidogenic function.
Title: Pharmacological doses of testosterone upregulated androgen receptor and 3-Beta-hydroxysteroid dehydrogenase/delta-5-delta-4 isomerase and impaired leydig cells steroidogenesis in adult rats.

compared (+)- and (-)-gossypols in the inhibition of 3beta-HSD and 17beta-HSD3 in human and rat testes
Title: The (+)- and (-)-gossypols potently inhibit both 3beta-hydroxysteroid dehydrogenase and 17beta-hydroxysteroid dehydrogenase 3 in human and rat testes.

L-tyrosine significantly inhibited 3beta-HSD activity in cultured luteal cells.
Title: [Effect of L-tyrosine on 3beta-HSD activity of rat luteal cells in vitro].

Data suggest that cAMP mediates transcriptional regulation of Hsd3b1 in Leydig cells (i.e., both normal Leydig cells and Leydig tumor cells); luteinizing hormone may be required to maintain expression of testicular Hsd3b1.
Title: Regulation of expression of 3?-hydroxysteroid dehydrogenase is mediated by cAMP in rat Leydig cells and H540 rat Leydig tumor cells.

The following Hsd3b1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Hsd3b1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa11836	NM_001007719.3 Latest version!	Rattus norvegicus hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 1 (Hsd3b1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$188.30 $269.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Hsd3b1 ( NM_001007719.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001007720.3 Rattus norvegicus hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 1 (Hsd3b1), mRNA.

Price & Availability↑

CloneID	ORa11836
Clone ID Related Accession (Same CDS sequence)	NM_001007719.3
Accession Version	NM_001007719.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1122bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Rattus norvegicus(Norway rat)
Product	3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC086578.1. On Feb 15, 2005 this sequence version replaced NM_001007719.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC086578.1, M38178.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2689601, SAMN02642892 [ECO:0000348] ##Evidence-Data-END##

ATGCCTGGAT GGAGCTGCCT GGTGACAGGA GCAGGAGGGT TTGTGGGCCA GAGGATCATC 
CGGATGTTGG TGCAGGAGAA AGAACTGCAG GAGGTCAGGG CTCTGGACAA AGTCTTCAGA 
CCAGAAACCA AGGAGGAATT CTCTAAGCTG CAGACAAAGG CCAAGGTGAC AATGTTAGAA 
GGAGATATTC TGGATGCCCA GTACCTGAGG AGAGCGTGCC AGGGCATCTC TGTTGTCATC 
CACACCGCTG CTGTCATTGA TGTCTCACAT GTCCTACCCA GGCAGACCAT CCTAGATGTC 
AATCTGAAAG GTACCCAGAA CATATTGGAG GCCTGCGTCG AAGCCAGTGT GCCAGCCTTC 
ATCTACTGCA GCACAGTTGA CGTTGCAGGG CCCAACTCCT ACAAGAAGAT CATCCTGAAT 
GGCCATGAGG AAGAGCATCA TGAAAGCACA TGGTCAGATG CATACCCATA CAGCAAAAGG 
ATGGCCGAGA AGGCAGTGCT GGCAGCCAAT GGGAGCATCC TGAAAAATGG TGGCACACTG 
CATACTTGTG CCTTAAGGCC CATGTACATT TATGGGGAGA GAAGTCCATT CCTTTCTGTC 
ATGATACTTG CGGCCCTCAA AAATAAGGGT ATTCTGAATG TTACTGGCAA ATTCTCCATA 
GCCAATCCAG TGTATGTAGG CAATGTGGCC TGGGCACATA TTCTGGCTGC CAGGGGCCTT 
CGAGACCCCA AGAAGTCACA AAATGTCCAA GGACAGTTCT ACTACATCTC AGATGACACC 
CCTCACCAAA GCTATGATGA TTTAAATTGC ACCCTGAGCA AGGAATGGGG CCTCCGCCTT 
GATTCCAGCT GGAGCCTTCC TCTGCCCCTG CTCTACTGGC TTGCCTTCCT GCTGGAAACT 
GTGAGCTTCC TGCTGCGTCC ATTTTACAAC TATAGGCCAC CCTTTAACTG CCACTTGGTC 
ACACTGTCAA ATAGCAAGTT CACTTTCTCC TACAAGAAAG CTCAGAGAGA TCTGGGCTAT 
GTGCCACTTG TCAGCTGGGA GGAAGCCAAG CAGAAAACCT CGGAGTGGAT CGGGACACTA 
GTGGAGCAGC ACAGGGAGAC ACTGGACACA AAGTCTCAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001007720.3
CDS	153..1274
Translation	MPGWSCLVTGAGGFVGQRIIRMLVQEKELQEVRALDKVFRPETKEEFSKLQTKAKVTMLEGDILDAQYLRRACQGISVVIHTAAVIDVSHVLPRQTILDVNLKGTQNILEACVEASVPAFIYCSTVDVAGPNSYKKIILNGHEEEHHESTWSDAYPYSKRMAEKAVLAANGSILKNGGTLHTCALRPMYIYGERSPFLSVMILAALKNKGILNVTGKFSIANPVYVGNVAWAHILAARGLRDPKKSQNVQGQFYYISDDTPHQSYDDLNCTLSKEWGLRLDSSWSLPLPLLYWLAFLLETVSFLLRPFYNYRPPFNCHLVTLSNSKFTFSYKKAQRDLGYVPLVSWEEAKQKTSEWIGTLVEQHRETLDTKSQ

Target ORF information:

RefSeq Version	NM_001007719.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 1 (Hsd3b1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001007719.3

ORF Insert Sequence:

ATGCCTGGAT GGAGCTGCCT GGTGACAGGA GCAGGAGGGT TTGTGGGCCA GAGGATCATC 
CGGATGTTGG TGCAGGAGAA AGAACTGCAG GAGGTCAGGG CTCTGGACAA AGTCTTCAGA 
CCAGAAACCA AGGAGGAATT CTCTAAGCTG CAGACAAAGG CCAAGGTGAC AATGTTAGAA 
GGAGATATTC TGGATGCCCA GTACCTGAGG AGAGCGTGCC AGGGCATCTC TGTTGTCATC 
CACACCGCTG CTGTCATTGA TGTCTCACAT GTCCTACCCA GGCAGACCAT CCTAGATGTC 
AATCTGAAAG GTACCCAGAA CATATTGGAG GCCTGCGTCG AAGCCAGTGT GCCAGCCTTC 
ATCTACTGCA GCACAGTTGA CGTTGCAGGG CCCAACTCCT ACAAGAAGAT CATCCTGAAT 
GGCCATGAGG AAGAGCATCA TGAAAGCACA TGGTCAGATG CATACCCATA CAGCAAAAGG 
ATGGCCGAGA AGGCAGTGCT GGCAGCCAAT GGGAGCATCC TGAAAAATGG TGGCACACTG 
CATACTTGTG CCTTAAGGCC CATGTACATT TATGGGGAGA GAAGTCCATT CCTTTCTGTC 
ATGATACTTG CGGCCCTCAA AAATAAGGGT ATTCTGAATG TTACTGGCAA ATTCTCCATA 
GCCAATCCAG TGTATGTAGG CAATGTGGCC TGGGCACATA TTCTGGCTGC CAGGGGCCTT 
CGAGACCCCA AGAAGTCACA AAATGTCCAA GGACAGTTCT ACTACATCTC AGATGACACC 
CCTCACCAAA GCTATGATGA TTTAAATTGC ACCCTGAGCA AGGAATGGGG CCTCCGCCTT 
GATTCCAGCT GGAGCCTTCC TCTGCCCCTG CTCTACTGGC TTGCCTTCCT GCTGGAAACT 
GTGAGCTTCC TGCTGCGTCC ATTTTACAAC TATAGGCCAC CCTTTAACTG CCACTTGGTC 
ACACTGTCAA ATAGCAAGTT CACTTTCTCC TACAAGAAAG CTCAGAGAGA TCTGGGCTAT 
GTGCCACTTG TCAGCTGGGA GGAAGCCAAG CAGAAAACCT CGGAGTGGAT CGGGACACTA 
GTGGAGCAGC ACAGGGAGAC ACTGGACACA AAGTCTCAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Different levels of leptin regulate different target enzymes involved in progesterone synthesis.
Fertility and sterility99(5)1460-6(2013 Apr)
Bilbao MG,Di Yorio MP,Faletti AG

Pharmacological doses of testosterone upregulated androgen receptor and 3-Beta-hydroxysteroid dehydrogenase/delta-5-delta-4 isomerase and impaired leydig cells steroidogenesis in adult rats.
Toxicological sciences : an official journal of the Society of Toxicology121(2)397-407(2011 Jun)
Kostic TS,Stojkov NJ,Bjelic MM,Mihajlovic AI,Janjic MM,Andric SA

Semicomprehensive analysis of the postnatal age-related changes in the mRNA expression of sex steroidogenic enzymes and sex steroid receptors in the male rat hippocampus.
Endocrinology151(12)5795-806(2010 Dec)
Kimoto T,Ishii H,Higo S,Hojo Y,Kawato S

[Effect of L-tyrosine on 3beta-HSD activity of rat luteal cells in vitro].
Zhongguo ying yong sheng li xue za zhi = Zhongguo yingyong shenglixue zazhi = Chinese journal of applied physiology20(2)190-3(2004 May)
Wang HP,Ni J,Cheng ZP

Regulation of expression of 3?-hydroxysteroid dehydrogenase is mediated by cAMP in rat Leydig cells and H540 rat Leydig tumor cells.
The Journal of steroid biochemistry and molecular biology43(8)915-22(1992 Dec)
Keeney DS,Mason JI

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Hsd3b1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

見積依頼

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FAX

オンラインフォーム

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