Brca2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Brca2
Entrez Gene ID	360254
Full Name	BRCA2, DNA repair associated
General protein information	Preferred Names BRCA2, DNA repair associated Names breast cancer type 2 susceptibility protein homolog breast cancer 2, early onset breast cancer 2, mutation 1, University of Wisconsin-Madison breast cancer susceptibility protein 2 fanconi anemia group D1 protein homolog
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 12 Map Location: 12p12
Summary	tumor suppression gene; involved in repair of double-strand DNA breaks (DSBs) by homologous recombination [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_017598372.1	XP_017453861.1	breast cancer type 2 susceptibility protein homolog isoform X1
XM_017598373.1	XP_017453862.1	breast cancer type 2 susceptibility protein homolog isoform X2
XM_017598375.1	XP_017453864.1	breast cancer type 2 susceptibility protein homolog isoform X4
XM_017598374.1	XP_017453863.1	breast cancer type 2 susceptibility protein homolog isoform X3
XM_017598376.1	XP_017453865.1	breast cancer type 2 susceptibility protein homolog isoform X5
NM_031542.2	NP_113730.2	breast cancer type 2 susceptibility protein homolog

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	BRCA2	NP_001006654.2
Mus musculus (house mouse)	Brca2	NP_033895.2
	BRCA2	XP_001118184.2
Homo sapiens (human)	BRCA2	NP_000050.2
Rattus norvegicus (Norway rat)	Brca2	NP_113730.2
Pan troglodytes (chimpanzee)	BRCA2	XP_509619.2
Bos taurus (cattle)	BRCA2	XP_002691853.1
Gallus gallus (chicken)	BRCA2	NP_989607.2

Gene Ontology
Bibliography

Related articles in PubMed

Genetic variants in fanconi anemia pathway genes BRCA2 and FANCA predict melanoma survival.
Yin J, Liu H, Liu Z, Wang LE, Chen WV, Zhu D, Amos CI, Fang S, Lee JE, Wei Q
The Journal of investigative dermatology135(2)542-550(2015 Feb)

Structure and mechanism of action of the BRCA2 breast cancer tumor suppressor.
Shahid T, Soroka J, Kong E, Malivert L, McIlwraith MJ, Pape T, West SC, Zhang X
Nature structural & molecular biology21(11)962-968(2014 Nov)

Risk of cancer other than breast or ovarian in individuals with BRCA1 and BRCA2 mutations.
Moran A, O'Hara C, Khan S, Shack L, Woodward E, Maher ER, Lalloo F, Evans DG
Familial cancer11(2)235-42(2012 Jun)

p53- and Bax-mediated apoptosis in injured rat spinal cord.
Kotipatruni RR, Dasari VR, Veeravalli KK, Dinh DH, Fassett D, Rao JS
Neurochemical research36(11)2063-74(2011 Nov)

A comprehensive functional characterization of BRCA2 variants associated with Fanconi anemia using mouse ES cell-based assay.
Biswas K, Das R, Alter BP, Kuznetsov SG, Stauffer S, North SL, Burkett S, Brody LC, Meyer S, Byrd RA, Sharan SK
Blood118(9)2430-42(2011 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Besides BRCA1/2 further high-risk breast cancer genes are known; however they account only for a small fraction of inherited breast cancer cases. Most of them are involved in rare cancer predisposition syndromes.
Title: [Genes beyond BRCA1 and BRCA2 for hereditary breast cancer].

Brca2-/- rats show a phenotype of growth inhibition and sterility in both sexes. Aspermatogenesis in the Brca2-/- rats is due to a failure of homologous chromosome synapsis
Title: Characterizing a rat Brca2 knockout model.

crystal structure of a COOH terminal BRCA2 domain bound to DSS1; demonstrate that this BRCA2 domain binds single-stranded DNA and show that BRCA2 stimulates RAD51-mediated recombination in vitro
Title: BRCA2 function in DNA binding and recombination from a BRCA2-DSS1-ssDNA structure.

The following Brca2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Brca2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa57848	XM_017598376.1 Latest version!	Rattus norvegicus BRCA2, DNA repair associated (Brca2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
ORa34515	NM_031542.2 Latest version!	Rattus norvegicus BRCA2, DNA repair associated (Brca2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
ORa34515	XM_017598372.1 Latest version!	Rattus norvegicus BRCA2, DNA repair associated (Brca2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
ORa57845	XM_017598373.1 Latest version!	Rattus norvegicus BRCA2, DNA repair associated (Brca2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
ORa57846	XM_017598374.1 Latest version!	Rattus norvegicus BRCA2, DNA repair associated (Brca2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
ORa57847	XM_017598375.1 Latest version!	Rattus norvegicus BRCA2, DNA repair associated (Brca2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Brca2 ( XM_017598376.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017453865.1 Rattus norvegicus BRCA2, DNA repair associated (Brca2), transcript variant X5, mRNA.

Price & Availability↑

CloneID	ORa57848
Clone ID Related Accession (Same CDS sequence)	XM_017598376.1
Accession Version	XM_017598376.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2802bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	breast cancer type 2 susceptibility protein homolog isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005111.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAAGCC TTCAGAATGC CAGAGACCTA CAGGATATAC GAATTAAAAA CAAAGAAAGG 
CATCATCTCT GTCCGCAGCC AGGCAGTCTG TATCTTACAA AATCATCCAC CCTGCCCCGG 
ATTTCTCTGC AGGCCGCAGT AGGAGACAGC GTTCCTTCTG CCTGTTCTCC TAAGCAGCTC 
TATATGTATG GCGTTTCTAA AGCATGCATA AGCGTTAACA GCAAAAACGC TGAGTATTTT 
CAGTTTGCCA TTGAGGATCA CTTTGGTAAA GAAGCTCTGT GCGCTGGCAA AGGCTTTCGG 
TTGGCAGATG GTGGATGGCT AATCCCCTCC GATGACGGGA AGGCTGGGAA AGAAGAATTT 
TACAGGGCTC TGTGTGACAC TCCAGGTGTG GATCCAAAGC TGATTTCTAG CGTCTGGGTC 
TCTAACCACT ACAGGTGGAT TGTATGGAAA CTGGCAGCTA TGGAATTTGC TTTTCCTAAG 
GAATTCGCGA ATAGATGTCT AAACCCAGAG CGAGTGCTGC TTCAACTAAA ATACAGATAC 
GATGTGGAAA TTGACAATAG CAGCAGATCA GCTCTCAAGA AGATACTGGA AAGGGATGAT 
ACAGCAGCAA AAACGCTTGT CCTCTGTGTT TCTGATATCA TTTCACTAAG CACAAATGTG 
TCTGAAACTT CAGGCAGTAA AGCTAGCAGT GAGGACAGTA ACAAAGTAGA CACGATCGAA 
CTCACAGATG GGTGGTATGC TGTCAAGGCC CAGCTAGACC CTCCACTCCT GGCTCTCGTA 
AAGAGTGGGA GACTCACTGT GGGTCAGAAG ATCATTACTC AGGGAGCAGA GCTGGTGGGC 
TCTCCCGATG CCTGTGCACC TCTGGAAGCC CCAGACTCCC TTAGGCTAAA GATTTCTGCA 
AACAGCACGC GGCCTGCTCG CTGGCACAGC AAGCTGGGGT TCTTTCATGA CCCCAGGCCC 
TTCCCTCTGC CCTTGTCCTC ACTGTTCAGT GATGGAGGAA ATGTTGGTTG TGTGGATGTC 
ATCGTTCAGA GAGTGTACCC TCTACAGTGG GTGGAGAAGA CGGTGTCTGG ATCGTACATA 
TTTCGTAATG AGAGAGAGGA GGAGAAGGAA GCGCTGAGAT TTGCAGAGGC CCAGCAGAAG 
AAACTAGAGG CCTTGTTCAC CAAAGTCCAC ACAGAGCTTA AAGAGCATGA AGAAGACATA 
GCCCAGCGGC GTGTGCTGTC CCGGGCACTC ACACGGCAGC AGGTCCACGC TCTGCAGGAC 
GGTGCAGAGC TCTATGCAGC AGTGCAGGAT GCATCAGACC CAGAGCACCT GGAGACTTGT 
TTCAGCGAAG AGCAGCTGAG AGCCTTGAAC AACTACAGAC AAATGCTGAG CGATAAGAAA 
CAAGCGCGGA TCCAGTCAGA GTTCCGGAAG GCCCTGGAGG CCGCTGAGAA AGAAGAGGGT 
TTATCAAGGG ACGTCTCAAC TGTGTGGAAG CTTCGTGTTA CAAGCTACAA GAAAAGAGAA 
AAATCAGCTC TGTTGAGTAT CTGGCGTCCA TCTTCAGACT TGCCCTCCCT GTTAACAGAA 
GGACAGAGAT ACAGAATCTA TCATCTTTCG GTGTCAAAAT CGAAGAATAA ATTTGAGTGG 
CCCAGCATCC AGTTAACAGC CACAAAGAGA ACTCAGTATC AACAGCTGCC GGTTTCCAGT 
GAGACCCTGC TCCAGCTTTA CCAGCCCAGG GAGCTCCTTC CCTTCAGCAA ACTGTCAGAT 
CCAGCCTTCC AGCCACCTTG TTCTGAAGTG GATGTAGTAG GAGTTGTAGT TTCTGTTGTA 
AAACCAATAG GTCTTGCTCC TTTGGTCTAC TTGTCAGACG AGTGCCTTCA TTTATTAGTG 
GTGAAATTTG GAATAGACCT TAATGAAGAC ATAAAGCCAC GTGTGCTTAT TGCTGCGAGC 
AACCTCCAGT GGCGGCCGGA GTCCACGTCA CGAGTGCCAA CTTTATTTGC TGGGAACTTT 
TCCGTATTCT CTGCCAGTCC AAAGGAGGCC CACTTTCAGG AGAGGGTCAC GAACATGAAG 
CATGCTATTG AGAACATCGA CACATTTTAC AAGGAGGCAG AAAAGAAGCT TATACAGGTG 
CTGAAGGGAG ACAGTCCAAA GTGGTCCACC CCGAACAAAG ACCCCACCCG AGAACCCTAC 
CCAGCCTCCA CTTGCTCTGC TTCAGACCTT GCTTCAGGAG GTCAGTTACC GAGGAGTTCA 
CCTACTGATC AGCAAAGTTA TCGAAGTCCT TTATCATGCT GCACACCGAC GGGGAAATCT 
ACACCCCTGG CCCACTCAGC CTGGATGGCA GCCAAGTCTT GTAGTGGGGA GAATGAGATT 
GAGGACCCCA AGACCTGTCG AAAAAAAAGG GCCTTGGATC TCCTAAGTCG GCTGCCCTTA 
CCGCCACCGC TCAGTCCTGT CTGCACCTTT GTCTCTCCGG CTGCACAGAA GGCCTTTCAG 
CCACCACGGA GCTGTGGCAC CAAATACCCA ACACCTCTAA AAAAAGAGGG ACCCAGTTCC 
CCTTGGAGCA GGGCGCCATT TCAGAAGGCC AGTGGCGTTT CTCTCCTGGA CTGTGATTCA 
GTAGCTGATG AAGAACTTGC CTTGCTCAGT ACCCAAGCCC TTGTGCCTCA CTCAGTGGGA 
GGAAGTGAAC AAGTGTTTCC CAGTGATTCC ACAAGGACAG AAGGACCCTC AGCCAGCACA 
GAGGCCAGAC CAGCAAATAG ATCCAAGAGG GAGTCTCTGA GGGACTGTAG AGATGACAGC 
GATGGGAAAT TGGCTGCTGA GACAGTTCCA GACTACTCAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017453865.1
CDS	342..3143
Translation	MSSLQNARDLQDIRIKNKERHHLCPQPGSLYLTKSSTLPRISLQAAVGDSVPSACSPKQLYMYGVSKACISVNSKNAEYFQFAIEDHFGKEALCAGKGFRLADGGWLIPSDDGKAGKEEFYRALCDTPGVDPKLISSVWVSNHYRWIVWKLAAMEFAFPKEFANRCLNPERVLLQLKYRYDVEIDNSSRSALKKILERDDTAAKTLVLCVSDIISLSTNVSETSGSKASSEDSNKVDTIELTDGWYAVKAQLDPPLLALVKSGRLTVGQKIITQGAELVGSPDACAPLEAPDSLRLKISANSTRPARWHSKLGFFHDPRPFPLPLSSLFSDGGNVGCVDVIVQRVYPLQWVEKTVSGSYIFRNEREEEKEALRFAEAQQKKLEALFTKVHTELKEHEEDIAQRRVLSRALTRQQVHALQDGAELYAAVQDASDPEHLETCFSEEQLRALNNYRQMLSDKKQARIQSEFRKALEAAEKEEGLSRDVSTVWKLRVTSYKKREKSALLSIWRPSSDLPSLLTEGQRYRIYHLSVSKSKNKFEWPSIQLTATKRTQYQQLPVSSETLLQLYQPRELLPFSKLSDPAFQPPCSEVDVVGVVVSVVKPIGLAPLVYLSDECLHLLVVKFGIDLNEDIKPRVLIAASNLQWRPESTSRVPTLFAGNFSVFSASPKEAHFQERVTNMKHAIENIDTFYKEAEKKLIQVLKGDSPKWSTPNKDPTREPYPASTCSASDLASGGQLPRSSPTDQQSYRSPLSCCTPTGKSTPLAHSAWMAAKSCSGENEIEDPKTCRKKRALDLLSRLPLPPPLSPVCTFVSPAAQKAFQPPRSCGTKYPTPLKKEGPSSPWSRAPFQKASGVSLLDCDSVADEELALLSTQALVPHSVGGSEQVFPSDSTRTEGPSASTEARPANRSKRESLRDCRDDSDGKLAAETVPDYS

Target ORF information:

RefSeq Version	XM_017598376.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus BRCA2, DNA repair associated (Brca2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017598376.1

ORF Insert Sequence:

ATGTCAAGCC TTCAGAATGC CAGAGACCTA CAGGATATAC GAATTAAAAA CAAAGAAAGG 
CATCATCTCT GTCCGCAGCC AGGCAGTCTG TATCTTACAA AATCATCCAC CCTGCCCCGG 
ATTTCTCTGC AGGCCGCAGT AGGAGACAGC GTTCCTTCTG CCTGTTCTCC TAAGCAGCTC 
TATATGTATG GCGTTTCTAA AGCATGCATA AGCGTTAACA GCAAAAACGC TGAGTATTTT 
CAGTTTGCCA TTGAGGATCA CTTTGGTAAA GAAGCTCTGT GCGCTGGCAA AGGCTTTCGG 
TTGGCAGATG GTGGATGGCT AATCCCCTCC GATGACGGGA AGGCTGGGAA AGAAGAATTT 
TACAGGGCTC TGTGTGACAC TCCAGGTGTG GATCCAAAGC TGATTTCTAG CGTCTGGGTC 
TCTAACCACT ACAGGTGGAT TGTATGGAAA CTGGCAGCTA TGGAATTTGC TTTTCCTAAG 
GAATTCGCGA ATAGATGTCT AAACCCAGAG CGAGTGCTGC TTCAACTAAA ATACAGATAC 
GATGTGGAAA TTGACAATAG CAGCAGATCA GCTCTCAAGA AGATACTGGA AAGGGATGAT 
ACAGCAGCAA AAACGCTTGT CCTCTGTGTT TCTGATATCA TTTCACTAAG CACAAATGTG 
TCTGAAACTT CAGGCAGTAA AGCTAGCAGT GAGGACAGTA ACAAAGTAGA CACGATCGAA 
CTCACAGATG GGTGGTATGC TGTCAAGGCC CAGCTAGACC CTCCACTCCT GGCTCTCGTA 
AAGAGTGGGA GACTCACTGT GGGTCAGAAG ATCATTACTC AGGGAGCAGA GCTGGTGGGC 
TCTCCCGATG CCTGTGCACC TCTGGAAGCC CCAGACTCCC TTAGGCTAAA GATTTCTGCA 
AACAGCACGC GGCCTGCTCG CTGGCACAGC AAGCTGGGGT TCTTTCATGA CCCCAGGCCC 
TTCCCTCTGC CCTTGTCCTC ACTGTTCAGT GATGGAGGAA ATGTTGGTTG TGTGGATGTC 
ATCGTTCAGA GAGTGTACCC TCTACAGTGG GTGGAGAAGA CGGTGTCTGG ATCGTACATA 
TTTCGTAATG AGAGAGAGGA GGAGAAGGAA GCGCTGAGAT TTGCAGAGGC CCAGCAGAAG 
AAACTAGAGG CCTTGTTCAC CAAAGTCCAC ACAGAGCTTA AAGAGCATGA AGAAGACATA 
GCCCAGCGGC GTGTGCTGTC CCGGGCACTC ACACGGCAGC AGGTCCACGC TCTGCAGGAC 
GGTGCAGAGC TCTATGCAGC AGTGCAGGAT GCATCAGACC CAGAGCACCT GGAGACTTGT 
TTCAGCGAAG AGCAGCTGAG AGCCTTGAAC AACTACAGAC AAATGCTGAG CGATAAGAAA 
CAAGCGCGGA TCCAGTCAGA GTTCCGGAAG GCCCTGGAGG CCGCTGAGAA AGAAGAGGGT 
TTATCAAGGG ACGTCTCAAC TGTGTGGAAG CTTCGTGTTA CAAGCTACAA GAAAAGAGAA 
AAATCAGCTC TGTTGAGTAT CTGGCGTCCA TCTTCAGACT TGCCCTCCCT GTTAACAGAA 
GGACAGAGAT ACAGAATCTA TCATCTTTCG GTGTCAAAAT CGAAGAATAA ATTTGAGTGG 
CCCAGCATCC AGTTAACAGC CACAAAGAGA ACTCAGTATC AACAGCTGCC GGTTTCCAGT 
GAGACCCTGC TCCAGCTTTA CCAGCCCAGG GAGCTCCTTC CCTTCAGCAA ACTGTCAGAT 
CCAGCCTTCC AGCCACCTTG TTCTGAAGTG GATGTAGTAG GAGTTGTAGT TTCTGTTGTA 
AAACCAATAG GTCTTGCTCC TTTGGTCTAC TTGTCAGACG AGTGCCTTCA TTTATTAGTG 
GTGAAATTTG GAATAGACCT TAATGAAGAC ATAAAGCCAC GTGTGCTTAT TGCTGCGAGC 
AACCTCCAGT GGCGGCCGGA GTCCACGTCA CGAGTGCCAA CTTTATTTGC TGGGAACTTT 
TCCGTATTCT CTGCCAGTCC AAAGGAGGCC CACTTTCAGG AGAGGGTCAC GAACATGAAG 
CATGCTATTG AGAACATCGA CACATTTTAC AAGGAGGCAG AAAAGAAGCT TATACAGGTG 
CTGAAGGGAG ACAGTCCAAA GTGGTCCACC CCGAACAAAG ACCCCACCCG AGAACCCTAC 
CCAGCCTCCA CTTGCTCTGC TTCAGACCTT GCTTCAGGAG GTCAGTTACC GAGGAGTTCA 
CCTACTGATC AGCAAAGTTA TCGAAGTCCT TTATCATGCT GCACACCGAC GGGGAAATCT 
ACACCCCTGG CCCACTCAGC CTGGATGGCA GCCAAGTCTT GTAGTGGGGA GAATGAGATT 
GAGGACCCCA AGACCTGTCG AAAAAAAAGG GCCTTGGATC TCCTAAGTCG GCTGCCCTTA 
CCGCCACCGC TCAGTCCTGT CTGCACCTTT GTCTCTCCGG CTGCACAGAA GGCCTTTCAG 
CCACCACGGA GCTGTGGCAC CAAATACCCA ACACCTCTAA AAAAAGAGGG ACCCAGTTCC 
CCTTGGAGCA GGGCGCCATT TCAGAAGGCC AGTGGCGTTT CTCTCCTGGA CTGTGATTCA 
GTAGCTGATG AAGAACTTGC CTTGCTCAGT ACCCAAGCCC TTGTGCCTCA CTCAGTGGGA 
GGAAGTGAAC AAGTGTTTCC CAGTGATTCC ACAAGGACAG AAGGACCCTC AGCCAGCACA 
GAGGCCAGAC CAGCAAATAG ATCCAAGAGG GAGTCTCTGA GGGACTGTAG AGATGACAGC 
GATGGGAAAT TGGCTGCTGA GACAGTTCCA GACTACTCAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Brca2 ( NM_031542.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_113730.2 Rattus norvegicus BRCA2, DNA repair associated (Brca2), mRNA.

Price & Availability↑

CloneID	ORa34515
Clone ID Related Accession (Same CDS sequence)	XM_017598372.1 , NM_031542.2
Accession Version	NM_031542.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 10032bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Rattus norvegicus(Norway rat)
Product	breast cancer type 2 susceptibility protein homolog
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AABR07034923.1. On Sep 27, 2011 this sequence version replaced NM_031542.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U89653.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN02642649 [ECO:0000348] ##Evidence-Data-END##

ATGACCGTTG AATACAAAAG GAGACCAACT TTTTGGGAAA TTTTTAAAGC GAGATGCAGC 
ACAGCAGATT TAGGACCAAT AAGCCTCAAT TGGTTTGAAG AACTTTCTTC AGAAGCCCCA 
CCATATAATA CTGAACCTCC AGAAGAATCT GAATATAAAC CCCAAGGCCA TGAACCACAG 
CTATTTAAAA CACCACAGAG GAATCCCTCT TACCATCAGT TTGCTTCAAC TCCAATAATG 
TTCAAAGAAC AAAGTCAAAC TCTACCACTA GACCAGTCAC CTTTCAAAGA ATTAGGGAAT 
GTTGTTGCAA ATAGTAAACG TAAACATCAC AGCAAAAAGA AGGCCAGAAA GGACCCTGTG 
GTAGATGTTG CCAGTCTGCC GCTGAAAGCT TGTCCCAGTG AAAGCCCTTG TACTCCGCGA 
TGCACACAGG TGGCACCGCA GCGAAGAAAG CCAGTGGTAT CTGGAAGTTT ATTCTATACA 
CCAAAACTTG AGGAGACACC AAAACATATT TCTGAAAGTC TGGGAGTTGA AGTGGATCCG 
GATATGTCTT GGACAAGTTC ATTAGCTACA CCACCAACCC TTAGTTCCAC TGTGCTCATA 
GCCCGAGATG AAGAAGCACA CAGAAATGCA TTTCCAGCCG ACTCTCCTGC TAGTTTGAAA 
AGCTATTTTT CTAACCACAA TGAAAGTCTG AAAAAGAATG ATAGATTTAT TCCCTCTGTG 
TCTGACAGTG AAAACAAAAG CCAGCAAGAA GCTTTTAGTC AGGGACTGGA GAAAATGTTA 
GGGGATTCAT CTAGCAAAAT AAATCGCTTC AGAGACTGCC TTAGAAAACC AATACCAAAT 
GTTCTAGAAG ATGGGGAGAC AGCTGTAGAT ACTTCAGGAG AAGATAGTTT CTCGTTATGT 
TTTCCTAAAC GTAGAACCAG AAATCTACAG AAAACGAGAA TGGGCAAGAT GAAGAAGAAA 
ATCTTCAGTG AAACAAGGAC TGATGGATTA AGTGAAGAAG CCAGAGGACA AGCTGATGAT 
AAAAACTCAT TTGCACTTGA AATTGAACCA AGAGATAGTG AGCCCTTAGA TCCATCTGTA 
ACAAACCAGA AGCCCCTTTA CAGCCAGAGT GGGGACATCT CCAGTGAAGC TGGCCAGTGT 
TCAGACAGTA TATGGTCTCA GCCAGATCCC TCTGGTCTAA ATGGAACCCA GACAAGAAAA 
ATACCCCTAC TCCATATTTC TTCTCATAAG CAAAGTATTT TAGAAGACTT CATAGATATG 
AAGAAGGAAG GTACTGGCTC TATTACTTTT CCTCATATTT CTAGTCTTCC AGAACCAGAA 
AAGATGTTCA GTGAGGAAAC TCTGGTAGAC AAGGAACATG AAGGACAGCA TCTTGAGTCA 
CTTGAAGACT CCATCTCAGG GAAACAAATG GTGTCTGGAA CGAGTCAAAC AGCCTGTCTC 
TCTCCGAGTA TCAGGAAGTC TATAGTCAAG ATGAGAGAGC CACTTGAAGA GACTTTGGAT 
ACTGTTTTCT CAGACAGTAT GACCAGCTCA GCCTTTACAG AAGAACTTGA CGCCTCTGCA 
GGGGGACTGG AAATACATAC TGCATGCTCA CAGAGAGAGG ATTCTTTATG TCCTAGTTCA 
GTGGACACTG GAAGCTGGCC AACCACTCTC ACTGACACTT CTGCAACTGT GAAAAATGCA 
GGTTTAATAA CCACTCTCAA AAATAAAAGA AGAAAGTTTA TTTACTCTGT AAGTGATGAT 
GCATCTCATC AAGGAAAAAA ACTACAGACA CAGAGACAGT CAGAGCTTAC TAACCTTTCA 
GCCCCGTTTG AAGCAAGTGC TTTTGAAGTA CCATTCCCCT TTACAAATGT AGATTCAGGT 
ATACCAGATT CTTCAATCAA AAGAAGCAAT TTACCAAATG ATCCTGAAGA GCCATCTTTG 
TCCTTGACCA ACTCTTTTGT GACTGCTGCC AGTAAAGAAA TTAGTTATAT TCATGCATTG 
ATATCTCAGG ATCTAAATGA CAAAGAAGCA ATACTCAGTG AAGAAAAGCC ACAGCCATAT 
ACAGCCCTAG AAGCTGACTT TCTGTCGTGC TTGCCAGAAA GATCATGTGA AAATGATCAA 
AAAAGTCCAA AAGTTTCCGA CCGAAAAGAA AAAGTCTTAG TCTCAGCATG TCGTCCTTCA 
GGAAGGCTTG CAGCAGCAGT GCAGCTCAGC AGCATTAGCT TTGACTCTCA GGAAAACCCT 
CTTGGTAGCC ACAACGTAAC AAGTACTCTT AAATTAACTC CCAGCCCGAA GACACCTCTG 
TCAAAGCCAG TTGTGGTTTC TAGAGGGAAA ATGTGTAAAA TGCCAGAGAA ACTGCAATGT 
AAGAGTTGTA AAGATAATAT TGAATTAAGC AAAAACATCC CTCTGGGGGT TAATGAAATG 
TGTGTCTTAA GTGAAAATTC TGAAACACCT GAGCTTCTGC CACCTCTAGA ATATATAACA 
GAAGTGTCCT CATCAGTGAA GTCACAGTTC AATCAAAATA CAAAAATAGC AGTCGTACAA 
AAGGACCAAA AAGACTCAAC TTTTATTTCA GAAGTAACAG TCCATATGAA TTCTGAAGAA 
CTTTTCCCAG AAAAGGAGAA TAATTTTGCT TTTCAAGTAA CCAATGAAAG CAATAAACCC 
AATATAGGAA GTACTGTGGA ATTCCAGGAA GAAGACCTCA GCCACACAAA AGGGCATAGT 
CTCAAGAACT CTCCCATGAC AGTAGATAGA GACCTAGATG ATGAGCAAGC AGGCCAAGTG 
TTGATTACAG AGGACTCAGA TTCATTAGCA GTAGTCCATG ATTGTACAAA GAAGAGCAGA 
AATACTATAG AGCAGCATCA GAAAGGAACT GCAGACAAAG ACTTCAAGTC AAATTCCTCC 
TTGTATTTGA AATCAGATGG GAACAATGAT TATTTAGACA AATGGTCAGA GTTCTTGGAT 
CCACTCATGA ACCATAAATT TGGAGGTAGC TTCAGAACAG CTTCCAATAA AGAAATAAAA 
CTTTCAGAGG ATAATGTCAA GAAAAGCAAA ATGTTCTTCA AAGATATCGA AGAACAGTAT 
CCTACTAGTT TAGATTGTAT TGACACTGTT AGTACCCTAC AATTAGCAAA CAAGAAGAGA 
CTAAGTGAAC CTCATACATT TGATTTGAAG TCAGGTACTA CTGTATCTAC ACAGTGTCAT 
AGTCAATCAT CTGTTTCTCA TGAAGATACT CACACAGCAC CTCAGATGTT ATCTTCAAAG 
CAAGATTTTC ATTCAAGTCA TAACTTAACG CCCAGCCAAA AAGCAGAAAT TACAGAACTG 
TCTACTATCT TGGAAGAATC AGGAAGTCAG TTTGAATTCA CACAGTTCAA AAATCCAAGC 
CACATAGCAC AGAATAATAC ATCTGCAGTG CTTGGAAACC AGATGGCTGT TGTAAGGACC 
GCTTCTGAGG AGTGGAAAGA TGTTGATCTT CATCTCCCAC TGAATCCCTC CTCTGTAGGT 
CAGATAGATC ACAACAAGAA ATTCGAATGT TTGGTTGGAG TTAAGCAAAG CTCTTCTCAC 
CTGTTAGAAG ACACTTGTAA CCAAAATACA TCTTGTTTTT TACCGATAAA AGAAATGGAG 
TTTGGAGGAT TTTGTTCTGC TCTTGGCACA AAACTTAGTG TGTCTAATGA GGCTCTGAGA 
AAGGCTATGA AACTGTTCAG TGACATTGAA AATATTAGTG AGGAGCCTTC TACAAAAGTA 
GGACCAAGAG GATTCTCTTC ATGTGCACAT CATGATTCTG TTGCTTCCGT GTTTAAGATA 
AAGAAACAAA ACACTGATAA AAGTTTTGAT GAGAAATCTA GTAAGTGCCA GGTAACAGTA 
CAAAATAATA AGGAAATGAC TACCTGTATT CTTGTTGATG AAAATCCTGA AAATTATGTA 
AAGAATATAA AACAAGATAA CAACTATACT GGCTCTCAAA GAAATGCTTA TAAATTAGAA 
AACTCTGATG TTAGTAAATC AAGTACAAGT GGCACAGTTT ATATTAATAA AGGTGACAGT 
GATTTACCTT TTGCTGCTGA AAAAGGCAAT AAGTATCCTG AGTCATGTAC CCAATATGTG 
AGGGAAGAAA ATGCACAAAT TAAGGAAAGT GTATCAGATT TAACATGTTT GGAAGTCATG 
AAAGCTGAGG AAACATGTCA TATGAAATCT TCAGATAAAG AACAATTACC TTCAGATAAG 
ATGGAACAAA ATATGAAAGA GTTTAATATA TCTTTTCAGA CTGCAAGCGG GAAAAATATC 
AGAGTCTCCA AAGAGTCACT AAATAAAAGT GTGAATATTT TAGATCAGGA AACAGAAGAC 
TTGACTGTCA CTTCAGATTC TTTGAATTCT AAAATTCTTT GTGGCATAAA TAAGGACAAA 
ATGCATATTT CATGTCACAA GAAATCAATC AATATTAAAA AGGTATTTGA AGAACATTTC 
CCAATTGGAA CTGTCAGTCA ATTACCAGCT CTTCAGCAGT ATCCTGAATA TGAAATAGAA 
AGTATCAAAG AACCTACTCT GTTGAGTTTT CATACAGCTA GTGGGAAAAA AGTCAAAATT 
ATGCAGGAAT CTTTGGACAA AGTGAAAAAT CTTTTTGATG AGACACAGTA TGTTAGGAAA 
ACCACCAATT TTGGTCATCA AGAATCAAAA CCCCTGAAGG ACAGAGAGGA CTATAAAGAA 
AGACTTACAT TAGCATATGA GAAAATTGAA GTAACTGCCT CAAAATGTGA AGAAATGCAG 
AACTTTGTCT CTAAGCAGAC TGAAATGCTA CCCCAGCAAA ATGATCATAT GTATAGGCAA 
ACTGAAAATC TCACATCAAA TGGTAGCTCT CCCAAAGTAC ATGGAAACAT AGAAAATAAA 
ATAGAAAAGA ATCCTAGAAT TTGCTGTATT TGTCAGTCCT CATACTTTGT CACTGAAGAT 
TCTGCTTTGG CATGTTATAC GGGGGACAGT AGAAAAACTT GTGTCGGAGA GTCTTCTCTG 
TCCAAAGGCA AAAAATGGCT TAGAGAACAA AGTGATAAGC TTGGAACAAG AAATACTATT 
GAAATCCAAT GTGTAAAGGA ACACACTGAA GATTTTGCAG GAAATGCCTT ATATGAACAT 
AGTTTAGTCA TTATCAGAAC TGAAATTGAT ACAAGTCATG TCTCTGAAAA CCAAGCTTCA 
ACCCTCTTTA GTGACCCTAA TGTGTGTCAC AGCTATCTAT CCCATTCTAG TTTTTGTCAT 
CATGATGATA TGCATAATGA TTCAGGATAT TTCTTAAAAG ATAAAATTGA TTCTGATGTC 
CAGCCAGACA TGAAGAATAC TGAAGGCAAT GCCATTTTCC CTAAAATATC TGCTACAAAA 
GAAATAAAAC TACACCCACA AACTGTAAAT GAAGAGTGTG TTCAAAAACT GGAGACTAAT 
GCTTCACCAT ATGCAAATAA AAATATAGCC ATTGACTCAG CTATGCTGGA TTTAAGGAAT 
TGTAAGGTAG GCTCACCTGT ATTCATTACA ACTCATTCAC AAGAAACTGT AAGAATGAAA 
GAGATATTCA CAGATAACTG TAGTAAAATA GTCGAACAAA ACAGGGAGAG TAAACCAGAC 
ACTTGCCAGA CAAGCTGTCA TAAAGCATTG GATAATTCAG AGGATTTTAT ATGTCCTAGC 
TCTTCAGGTG ATGTCTGCAT AAACTCACCT ATGGCTATTT TTTATCCTCA AAGTGAACAA 
ATTTTACAGC ATAACCAAAG TGTGTCTGGA CTGAAGAAAG CTGCAACACC ACCTGTTAGT 
TTGGAAACTT GGGATACATG TAAATCTATA AGAGGATCTC CTCAGGAAGT CCATCCTTCA 
CGCACTTATG GAATTTTTAG CACAGCAAGT GGAAAAGCTG TACAAGTATC TGATGCTTCA 
TTGGAAAAGG CACGGCAAGT GTTTTCTGAG ATAGATGGTG ATGCTAAACA GTTAGCTTCC 
ATGGTGTCAC TGGAAGGTAA TGAAAAATCA CATCACTCTG TGAAAAGAGA AAGCTCTGTG 
GTGCATAACA CCCATGGTGT ATTGTCACTC CGAAAAACCC TCCCAGGCAA TGTCAGTTCA 
TCTGTATTCT CTGGATTTAG CACCGCAGGT GGAAAACTGG TCACAGTTTC AGAAAGTGCT 
TTACATAAAG TTAAAGGAAT GTTAGAGGAA TTTGATTTGA TCAGAACTGA ACATACTCTC 
CAGCATTCAC CTACACCTGA AGACGTATCA AAAATACCTC CTCAACCTTG TCTTGAAAGC 
AGAACCCCAG AATACTCTGT AAGCTCTAAA TTGCAGAAAA CCTACAATGA TAAATCCAGG 
TCACCAAGTA ATTATAAAGA AAGTGGTTCT TCAGGCAATA CTCAATCTCT TGAAGTTTCT 
CCCCAACTCT CTCAGATGGA GAGAAAGCAA GAAACACAGT CGGTATTAGG AACAAAAGTG 
TCCCAGAGGA AGACTAATAT CTTGGAAAAA AAACAAAACT TACCCCAAAA CATAAAAATA 
GAAAGTAATA AAATGGAAAC ATTTTCTGAT GTTTCCATGA AAACAAATGT AGGAGAGTAC 
TACTCCAAAG AGCCAGAGAA CTATTTTGAA ACAGAAGCAG TGGAAATTGC CAAAGCTTTT 
ATGGAAGATG ATGAGCTGAC GGATTCTGAA CAGACTCATG CCAAATGCTC ACTGTTTGCA 
TGCCCCCAAA ACGAGGCTTT ATTAAATTCG AGAACTAGAA AAAGAGGAGG AATGGCTGGT 
GTTGCAGTTG GACAACCCCC AATTAAAAGG AGCTTATTAA ATGAATTTGA CAGGATAATA 
GAAAGTAAAG GAAAATCCTT AACGCCTTCA AAAAGCACTC CAGATGGCAC AATAAAAGAC 
AGACGATTGT TCACACACCA CATGTCTTTA GAGCCGGTTA CCTGTGGACC CTTCTGCTCA 
AGTAAAGAAA GGCAAGAAAC CCAGAGCCCA CATGTTACCT CACCTGCTCA AGGACTTCAG 
TCTAAGGGGC ATCCTTCAAG ACACTCAGCT GTGGGAAAGT CTTCAAGCAA TCCTACAGTT 
TCTGCCCTAA GATCTGAAAG GACCAGACAC TCAGTCTCAG ACAAATCCAC CAAAGTCTTT 
GTCCCACCTT TCAAGGTGAA ATCACGGTTT CACAGAGATG AACATTTTGA TAGCAAGAAT 
GTTAATTTGG AGGGAAAAAA CCAAAAGAGC GCAGATGGAG TCAGTGAAGA TGGGAACGAC 
AGTGACTTTC CTCAGTTTAA CAAAGATTTA ATGTCAAGCC TTCAGAATGC CAGAGACCTA 
CAGGATATAC GAATTAAAAA CAAAGAAAGG CATCATCTCT GTCCGCAGCC AGGCAGTCTG 
TATCTTACAA AATCATCCAC CCTGCCCCGG ATTTCTCTGC AGGCCGCAGT AGGAGACAGC 
GTTCCTTCTG CCTGTTCTCC TAAGCAGCTC TATATGTATG GCGTTTCTAA AGCATGCATA 
AGCGTTAACA GCAAAAACGC TGAGTATTTT CAGTTTGCCA TTGAGGATCA CTTTGGTAAA 
GAAGCTCTGT GCGCTGGCAA AGGCTTTCGG TTGGCAGATG GTGGATGGCT AATCCCCTCC 
GATGACGGGA AGGCTGGGAA AGAAGAATTT TACAGGGCTC TGTGTGACAC TCCAGGTGTG 
GATCCAAAGC TGATTTCTAG CGTCTGGGTC TCTAACCACT ACAGGTGGAT TGTATGGAAA 
CTGGCAGCTA TGGAATTTGC TTTTCCTAAG GAATTCGCGA ATAGATGTCT AAACCCAGAG 
CGAGTGCTGC TTCAACTAAA ATACAGATAC GATGTGGAAA TTGACAATAG CAGCAGATCA 
GCTCTCAAGA AGATACTGGA AAGGGATGAT ACAGCAGCAA AAACGCTTGT CCTCTGTGTT 
TCTGATATCA TTTCACTAAG CACAAATGTG TCTGAAACTT CAGGCAGTAA AGCTAGCAGT 
GAGGACAGTA ACAAAGTAGA CACGATCGAA CTCACAGATG GGTGGTATGC TGTCAAGGCC 
CAGCTAGACC CTCCACTCCT GGCTCTCGTA AAGAGTGGGA GACTCACTGT GGGTCAGAAG 
ATCATTACTC AGGGAGCAGA GCTGGTGGGC TCTCCCGATG CCTGTGCACC TCTGGAAGCC 
CCAGACTCCC TTAGGCTAAA GATTTCTGCA AACAGCACGC GGCCTGCTCG CTGGCACAGC 
AAGCTGGGGT TCTTTCATGA CCCCAGGCCC TTCCCTCTGC CCTTGTCCTC ACTGTTCAGT 
GATGGAGGAA ATGTTGGTTG TGTGGATGTC ATCGTTCAGA GAGTGTACCC TCTACAGTGG 
GTGGAGAAGA CGGTGTCTGG ATCGTACATA TTTCGTAATG AGAGAGAGGA GGAGAAGGAA 
GCGCTGAGAT TTGCAGAGGC CCAGCAGAAG AAACTAGAGG CCTTGTTCAC CAAAGTCCAC 
ACAGAGCTTA AAGAGCATGA AGAAGACATA GCCCAGCGGC GTGTGCTGTC CCGGGCACTC 
ACACGGCAGC AGGTCCACGC TCTGCAGGAC GGTGCAGAGC TCTATGCAGC AGTGCAGGAT 
GCATCAGACC CAGAGCACCT GGAGACTTGT TTCAGCGAAG AGCAGCTGAG AGCCTTGAAC 
AACTACAGAC AAATGCTGAG CGATAAGAAA CAAGCGCGGA TCCAGTCAGA GTTCCGGAAG 
GCCCTGGAGG CCGCTGAGAA AGAAGAGGGT TTATCAAGGG ACGTCTCAAC TGTGTGGAAG 
CTTCGTGTTA CAAGCTACAA GAAAAGAGAA AAATCAGCTC TGTTGAGTAT CTGGCGTCCA 
TCTTCAGACT TGCCCTCCCT GTTAACAGAA GGACAGAGAT ACAGAATCTA TCATCTTTCG 
GTGTCAAAAT CGAAGAATAA ATTTGAGTGG CCCAGCATCC AGTTAACAGC CACAAAGAGA 
ACTCAGTATC AACAGCTGCC GGTTTCCAGT GAGACCCTGC TCCAGCTTTA CCAGCCCAGG 
GAGCTCCTTC CCTTCAGCAA ACTGTCAGAT CCAGCCTTCC AGCCACCTTG TTCTGAAGTG 
GATGTAGTAG GAGTTGTAGT TTCTGTTGTA AAACCAATAG GTCTTGCTCC TTTGGTCTAC 
TTGTCAGACG AGTGCCTTCA TTTATTAGTG GTGAAATTTG GAATAGACCT TAATGAAGAC 
ATAAAGCCAC GTGTGCTTAT TGCTGCGAGC AACCTCCAGT GGCGGCCGGA GTCCACGTCA 
CGAGTGCCAA CTTTATTTGC TGGGAACTTT TCCGTATTCT CTGCCAGTCC AAAGGAGGCC 
CACTTTCAGG AGAGGGTCAC GAACATGAAG CATGCTATTG AGAACATCGA CACATTTTAC 
AAGGAGGCAG AAAAGAAGCT TATACAGGTG CTGAAGGGAG ACAGTCCAAA GTGGTCCACC 
CCGAACAAAG ACCCCACCCG AGAACCCTAC CCAGCCTCCA CTTGCTCTGC TTCAGACCTT 
GCTTCAGGAG GTCAGTTACC GAGGAGTTCA CCTACTGATC AGCAAAGTTA TCGAAGTCCT 
TTATCATGCT GCACACCGAC GGGGAAATCT ACACCCCTGG CCCACTCAGC CTGGATGGCA 
GCCAAGTCTT GTAGTGGGGA GAATGAGATT GAGGACCCCA AGACCTGTCG AAAAAAAAGG 
GCCTTGGATC TCCTAAGTCG GCTGCCCTTA CCGCCACCGC TCAGTCCTGT CTGCACCTTT 
GTCTCTCCGG CTGCACAGAA GGCCTTTCAG CCACCACGGA GCTGTGGCAC CAAATACCCA 
ACACCTCTAA AAAAAGAGGG ACCCAGTTCC CCTTGGAGCA GGGCGCCATT TCAGAAGGCC 
AGTGGCGTTT CTCTCCTGGA CTGTGATTCA GTAGCTGATG AAGAACTTGC CTTGCTCAGT 
ACCCAAGCCC TTGTGCCTCA CTCAGTGGGA GGAAGTGAAC AAGTGTTTCC CAGTGATTCC 
ACAAGGACAG AAGGACCCTC AGCCAGCACA GAGGCCAGAC CAGCAAATAG ATCCAAGAGG 
GAGTCTCTGA GGGACTGTAG AGATGACAGC GATGGGAAAT TGGCTGCTGA GACAGTTCCA 
GACTACTCAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_113730.2
CDS	179..10210
Translation	MTVEYKRRPTFWEIFKARCSTADLGPISLNWFEELSSEAPPYNTEPPEESEYKPQGHEPQLFKTPQRNPSYHQFASTPIMFKEQSQTLPLDQSPFKELGNVVANSKRKHHSKKKARKDPVVDVASLPLKACPSESPCTPRCTQVAPQRRKPVVSGSLFYTPKLEETPKHISESLGVEVDPDMSWTSSLATPPTLSSTVLIARDEEAHRNAFPADSPASLKSYFSNHNESLKKNDRFIPSVSDSENKSQQEAFSQGLEKMLGDSSSKINRFRDCLRKPIPNVLEDGETAVDTSGEDSFSLCFPKRRTRNLQKTRMGKMKKKIFSETRTDGLSEEARGQADDKNSFALEIEPRDSEPLDPSVTNQKPLYSQSGDISSEAGQCSDSIWSQPDPSGLNGTQTRKIPLLHISSHKQSILEDFIDMKKEGTGSITFPHISSLPEPEKMFSEETLVDKEHEGQHLESLEDSISGKQMVSGTSQTACLSPSIRKSIVKMREPLEETLDTVFSDSMTSSAFTEELDASAGGLEIHTACSQREDSLCPSSVDTGSWPTTLTDTSATVKNAGLITTLKNKRRKFIYSVSDDASHQGKKLQTQRQSELTNLSAPFEASAFEVPFPFTNVDSGIPDSSIKRSNLPNDPEEPSLSLTNSFVTAASKEISYIHALISQDLNDKEAILSEEKPQPYTALEADFLSCLPERSCENDQKSPKVSDRKEKVLVSACRPSGRLAAAVQLSSISFDSQENPLGSHNVTSTLKLTPSPKTPLSKPVVVSRGKMCKMPEKLQCKSCKDNIELSKNIPLGVNEMCVLSENSETPELLPPLEYITEVSSSVKSQFNQNTKIAVVQKDQKDSTFISEVTVHMNSEELFPEKENNFAFQVTNESNKPNIGSTVEFQEEDLSHTKGHSLKNSPMTVDRDLDDEQAGQVLITEDSDSLAVVHDCTKKSRNTIEQHQKGTADKDFKSNSSLYLKSDGNNDYLDKWSEFLDPLMNHKFGGSFRTASNKEIKLSEDNVKKSKMFFKDIEEQYPTSLDCIDTVSTLQLANKKRLSEPHTFDLKSGTTVSTQCHSQSSVSHEDTHTAPQMLSSKQDFHSSHNLTPSQKAEITELSTILEESGSQFEFTQFKNPSHIAQNNTSAVLGNQMAVVRTASEEWKDVDLHLPLNPSSVGQIDHNKKFECLVGVKQSSSHLLEDTCNQNTSCFLPIKEMEFGGFCSALGTKLSVSNEALRKAMKLFSDIENISEEPSTKVGPRGFSSCAHHDSVASVFKIKKQNTDKSFDEKSSKCQVTVQNNKEMTTCILVDENPENYVKNIKQDNNYTGSQRNAYKLENSDVSKSSTSGTVYINKGDSDLPFAAEKGNKYPESCTQYVREENAQIKESVSDLTCLEVMKAEETCHMKSSDKEQLPSDKMEQNMKEFNISFQTASGKNIRVSKESLNKSVNILDQETEDLTVTSDSLNSKILCGINKDKMHISCHKKSINIKKVFEEHFPIGTVSQLPALQQYPEYEIESIKEPTLLSFHTASGKKVKIMQESLDKVKNLFDETQYVRKTTNFGHQESKPLKDREDYKERLTLAYEKIEVTASKCEEMQNFVSKQTEMLPQQNDHMYRQTENLTSNGSSPKVHGNIENKIEKNPRICCICQSSYFVTEDSALACYTGDSRKTCVGESSLSKGKKWLREQSDKLGTRNTIEIQCVKEHTEDFAGNALYEHSLVIIRTEIDTSHVSENQASTLFSDPNVCHSYLSHSSFCHHDDMHNDSGYFLKDKIDSDVQPDMKNTEGNAIFPKISATKEIKLHPQTVNEECVQKLETNASPYANKNIAIDSAMLDLRNCKVGSPVFITTHSQETVRMKEIFTDNCSKIVEQNRESKPDTCQTSCHKALDNSEDFICPSSSGDVCINSPMAIFYPQSEQILQHNQSVSGLKKAATPPVSLETWDTCKSIRGSPQEVHPSRTYGIFSTASGKAVQVSDASLEKARQVFSEIDGDAKQLASMVSLEGNEKSHHSVKRESSVVHNTHGVLSLRKTLPGNVSSSVFSGFSTAGGKLVTVSESALHKVKGMLEEFDLIRTEHTLQHSPTPEDVSKIPPQPCLESRTPEYSVSSKLQKTYNDKSRSPSNYKESGSSGNTQSLEVSPQLSQMERKQETQSVLGTKVSQRKTNILEKKQNLPQNIKIESNKMETFSDVSMKTNVGEYYSKEPENYFETEAVEIAKAFMEDDELTDSEQTHAKCSLFACPQNEALLNSRTRKRGGMAGVAVGQPPIKRSLLNEFDRIIESKGKSLTPSKSTPDGTIKDRRLFTHHMSLEPVTCGPFCSSKERQETQSPHVTSPAQGLQSKGHPSRHSAVGKSSSNPTVSALRSERTRHSVSDKSTKVFVPPFKVKSRFHRDEHFDSKNVNLEGKNQKSADGVSEDGNDSDFPQFNKDLMSSLQNARDLQDIRIKNKERHHLCPQPGSLYLTKSSTLPRISLQAAVGDSVPSACSPKQLYMYGVSKACISVNSKNAEYFQFAIEDHFGKEALCAGKGFRLADGGWLIPSDDGKAGKEEFYRALCDTPGVDPKLISSVWVSNHYRWIVWKLAAMEFAFPKEFANRCLNPERVLLQLKYRYDVEIDNSSRSALKKILERDDTAAKTLVLCVSDIISLSTNVSETSGSKASSEDSNKVDTIELTDGWYAVKAQLDPPLLALVKSGRLTVGQKIITQGAELVGSPDACAPLEAPDSLRLKISANSTRPARWHSKLGFFHDPRPFPLPLSSLFSDGGNVGCVDVIVQRVYPLQWVEKTVSGSYIFRNEREEEKEALRFAEAQQKKLEALFTKVHTELKEHEEDIAQRRVLSRALTRQQVHALQDGAELYAAVQDASDPEHLETCFSEEQLRALNNYRQMLSDKKQARIQSEFRKALEAAEKEEGLSRDVSTVWKLRVTSYKKREKSALLSIWRPSSDLPSLLTEGQRYRIYHLSVSKSKNKFEWPSIQLTATKRTQYQQLPVSSETLLQLYQPRELLPFSKLSDPAFQPPCSEVDVVGVVVSVVKPIGLAPLVYLSDECLHLLVVKFGIDLNEDIKPRVLIAASNLQWRPESTSRVPTLFAGNFSVFSASPKEAHFQERVTNMKHAIENIDTFYKEAEKKLIQVLKGDSPKWSTPNKDPTREPYPASTCSASDLASGGQLPRSSPTDQQSYRSPLSCCTPTGKSTPLAHSAWMAAKSCSGENEIEDPKTCRKKRALDLLSRLPLPPPLSPVCTFVSPAAQKAFQPPRSCGTKYPTPLKKEGPSSPWSRAPFQKASGVSLLDCDSVADEELALLSTQALVPHSVGGSEQVFPSDSTRTEGPSASTEARPANRSKRESLRDCRDDSDGKLAAETVPDYS

Target ORF information:

RefSeq Version	NM_031542.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus BRCA2, DNA repair associated (Brca2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_031542.2

ORF Insert Sequence:

ATGACCGTTG AATACAAAAG GAGACCAACT TTTTGGGAAA TTTTTAAAGC GAGATGCAGC 
ACAGCAGATT TAGGACCAAT AAGCCTCAAT TGGTTTGAAG AACTTTCTTC AGAAGCCCCA 
CCATATAATA CTGAACCTCC AGAAGAATCT GAATATAAAC CCCAAGGCCA TGAACCACAG 
CTATTTAAAA CACCACAGAG GAATCCCTCT TACCATCAGT TTGCTTCAAC TCCAATAATG 
TTCAAAGAAC AAAGTCAAAC TCTACCACTA GACCAGTCAC CTTTCAAAGA ATTAGGGAAT 
GTTGTTGCAA ATAGTAAACG TAAACATCAC AGCAAAAAGA AGGCCAGAAA GGACCCTGTG 
GTAGATGTTG CCAGTCTGCC GCTGAAAGCT TGTCCCAGTG AAAGCCCTTG TACTCCGCGA 
TGCACACAGG TGGCACCGCA GCGAAGAAAG CCAGTGGTAT CTGGAAGTTT ATTCTATACA 
CCAAAACTTG AGGAGACACC AAAACATATT TCTGAAAGTC TGGGAGTTGA AGTGGATCCG 
GATATGTCTT GGACAAGTTC ATTAGCTACA CCACCAACCC TTAGTTCCAC TGTGCTCATA 
GCCCGAGATG AAGAAGCACA CAGAAATGCA TTTCCAGCCG ACTCTCCTGC TAGTTTGAAA 
AGCTATTTTT CTAACCACAA TGAAAGTCTG AAAAAGAATG ATAGATTTAT TCCCTCTGTG 
TCTGACAGTG AAAACAAAAG CCAGCAAGAA GCTTTTAGTC AGGGACTGGA GAAAATGTTA 
GGGGATTCAT CTAGCAAAAT AAATCGCTTC AGAGACTGCC TTAGAAAACC AATACCAAAT 
GTTCTAGAAG ATGGGGAGAC AGCTGTAGAT ACTTCAGGAG AAGATAGTTT CTCGTTATGT 
TTTCCTAAAC GTAGAACCAG AAATCTACAG AAAACGAGAA TGGGCAAGAT GAAGAAGAAA 
ATCTTCAGTG AAACAAGGAC TGATGGATTA AGTGAAGAAG CCAGAGGACA AGCTGATGAT 
AAAAACTCAT TTGCACTTGA AATTGAACCA AGAGATAGTG AGCCCTTAGA TCCATCTGTA 
ACAAACCAGA AGCCCCTTTA CAGCCAGAGT GGGGACATCT CCAGTGAAGC TGGCCAGTGT 
TCAGACAGTA TATGGTCTCA GCCAGATCCC TCTGGTCTAA ATGGAACCCA GACAAGAAAA 
ATACCCCTAC TCCATATTTC TTCTCATAAG CAAAGTATTT TAGAAGACTT CATAGATATG 
AAGAAGGAAG GTACTGGCTC TATTACTTTT CCTCATATTT CTAGTCTTCC AGAACCAGAA 
AAGATGTTCA GTGAGGAAAC TCTGGTAGAC AAGGAACATG AAGGACAGCA TCTTGAGTCA 
CTTGAAGACT CCATCTCAGG GAAACAAATG GTGTCTGGAA CGAGTCAAAC AGCCTGTCTC 
TCTCCGAGTA TCAGGAAGTC TATAGTCAAG ATGAGAGAGC CACTTGAAGA GACTTTGGAT 
ACTGTTTTCT CAGACAGTAT GACCAGCTCA GCCTTTACAG AAGAACTTGA CGCCTCTGCA 
GGGGGACTGG AAATACATAC TGCATGCTCA CAGAGAGAGG ATTCTTTATG TCCTAGTTCA 
GTGGACACTG GAAGCTGGCC AACCACTCTC ACTGACACTT CTGCAACTGT GAAAAATGCA 
GGTTTAATAA CCACTCTCAA AAATAAAAGA AGAAAGTTTA TTTACTCTGT AAGTGATGAT 
GCATCTCATC AAGGAAAAAA ACTACAGACA CAGAGACAGT CAGAGCTTAC TAACCTTTCA 
GCCCCGTTTG AAGCAAGTGC TTTTGAAGTA CCATTCCCCT TTACAAATGT AGATTCAGGT 
ATACCAGATT CTTCAATCAA AAGAAGCAAT TTACCAAATG ATCCTGAAGA GCCATCTTTG 
TCCTTGACCA ACTCTTTTGT GACTGCTGCC AGTAAAGAAA TTAGTTATAT TCATGCATTG 
ATATCTCAGG ATCTAAATGA CAAAGAAGCA ATACTCAGTG AAGAAAAGCC ACAGCCATAT 
ACAGCCCTAG AAGCTGACTT TCTGTCGTGC TTGCCAGAAA GATCATGTGA AAATGATCAA 
AAAAGTCCAA AAGTTTCCGA CCGAAAAGAA AAAGTCTTAG TCTCAGCATG TCGTCCTTCA 
GGAAGGCTTG CAGCAGCAGT GCAGCTCAGC AGCATTAGCT TTGACTCTCA GGAAAACCCT 
CTTGGTAGCC ACAACGTAAC AAGTACTCTT AAATTAACTC CCAGCCCGAA GACACCTCTG 
TCAAAGCCAG TTGTGGTTTC TAGAGGGAAA ATGTGTAAAA TGCCAGAGAA ACTGCAATGT 
AAGAGTTGTA AAGATAATAT TGAATTAAGC AAAAACATCC CTCTGGGGGT TAATGAAATG 
TGTGTCTTAA GTGAAAATTC TGAAACACCT GAGCTTCTGC CACCTCTAGA ATATATAACA 
GAAGTGTCCT CATCAGTGAA GTCACAGTTC AATCAAAATA CAAAAATAGC AGTCGTACAA 
AAGGACCAAA AAGACTCAAC TTTTATTTCA GAAGTAACAG TCCATATGAA TTCTGAAGAA 
CTTTTCCCAG AAAAGGAGAA TAATTTTGCT TTTCAAGTAA CCAATGAAAG CAATAAACCC 
AATATAGGAA GTACTGTGGA ATTCCAGGAA GAAGACCTCA GCCACACAAA AGGGCATAGT 
CTCAAGAACT CTCCCATGAC AGTAGATAGA GACCTAGATG ATGAGCAAGC AGGCCAAGTG 
TTGATTACAG AGGACTCAGA TTCATTAGCA GTAGTCCATG ATTGTACAAA GAAGAGCAGA 
AATACTATAG AGCAGCATCA GAAAGGAACT GCAGACAAAG ACTTCAAGTC AAATTCCTCC 
TTGTATTTGA AATCAGATGG GAACAATGAT TATTTAGACA AATGGTCAGA GTTCTTGGAT 
CCACTCATGA ACCATAAATT TGGAGGTAGC TTCAGAACAG CTTCCAATAA AGAAATAAAA 
CTTTCAGAGG ATAATGTCAA GAAAAGCAAA ATGTTCTTCA AAGATATCGA AGAACAGTAT 
CCTACTAGTT TAGATTGTAT TGACACTGTT AGTACCCTAC AATTAGCAAA CAAGAAGAGA 
CTAAGTGAAC CTCATACATT TGATTTGAAG TCAGGTACTA CTGTATCTAC ACAGTGTCAT 
AGTCAATCAT CTGTTTCTCA TGAAGATACT CACACAGCAC CTCAGATGTT ATCTTCAAAG 
CAAGATTTTC ATTCAAGTCA TAACTTAACG CCCAGCCAAA AAGCAGAAAT TACAGAACTG 
TCTACTATCT TGGAAGAATC AGGAAGTCAG TTTGAATTCA CACAGTTCAA AAATCCAAGC 
CACATAGCAC AGAATAATAC ATCTGCAGTG CTTGGAAACC AGATGGCTGT TGTAAGGACC 
GCTTCTGAGG AGTGGAAAGA TGTTGATCTT CATCTCCCAC TGAATCCCTC CTCTGTAGGT 
CAGATAGATC ACAACAAGAA ATTCGAATGT TTGGTTGGAG TTAAGCAAAG CTCTTCTCAC 
CTGTTAGAAG ACACTTGTAA CCAAAATACA TCTTGTTTTT TACCGATAAA AGAAATGGAG 
TTTGGAGGAT TTTGTTCTGC TCTTGGCACA AAACTTAGTG TGTCTAATGA GGCTCTGAGA 
AAGGCTATGA AACTGTTCAG TGACATTGAA AATATTAGTG AGGAGCCTTC TACAAAAGTA 
GGACCAAGAG GATTCTCTTC ATGTGCACAT CATGATTCTG TTGCTTCCGT GTTTAAGATA 
AAGAAACAAA ACACTGATAA AAGTTTTGAT GAGAAATCTA GTAAGTGCCA GGTAACAGTA 
CAAAATAATA AGGAAATGAC TACCTGTATT CTTGTTGATG AAAATCCTGA AAATTATGTA 
AAGAATATAA AACAAGATAA CAACTATACT GGCTCTCAAA GAAATGCTTA TAAATTAGAA 
AACTCTGATG TTAGTAAATC AAGTACAAGT GGCACAGTTT ATATTAATAA AGGTGACAGT 
GATTTACCTT TTGCTGCTGA AAAAGGCAAT AAGTATCCTG AGTCATGTAC CCAATATGTG 
AGGGAAGAAA ATGCACAAAT TAAGGAAAGT GTATCAGATT TAACATGTTT GGAAGTCATG 
AAAGCTGAGG AAACATGTCA TATGAAATCT TCAGATAAAG AACAATTACC TTCAGATAAG 
ATGGAACAAA ATATGAAAGA GTTTAATATA TCTTTTCAGA CTGCAAGCGG GAAAAATATC 
AGAGTCTCCA AAGAGTCACT AAATAAAAGT GTGAATATTT TAGATCAGGA AACAGAAGAC 
TTGACTGTCA CTTCAGATTC TTTGAATTCT AAAATTCTTT GTGGCATAAA TAAGGACAAA 
ATGCATATTT CATGTCACAA GAAATCAATC AATATTAAAA AGGTATTTGA AGAACATTTC 
CCAATTGGAA CTGTCAGTCA ATTACCAGCT CTTCAGCAGT ATCCTGAATA TGAAATAGAA 
AGTATCAAAG AACCTACTCT GTTGAGTTTT CATACAGCTA GTGGGAAAAA AGTCAAAATT 
ATGCAGGAAT CTTTGGACAA AGTGAAAAAT CTTTTTGATG AGACACAGTA TGTTAGGAAA 
ACCACCAATT TTGGTCATCA AGAATCAAAA CCCCTGAAGG ACAGAGAGGA CTATAAAGAA 
AGACTTACAT TAGCATATGA GAAAATTGAA GTAACTGCCT CAAAATGTGA AGAAATGCAG 
AACTTTGTCT CTAAGCAGAC TGAAATGCTA CCCCAGCAAA ATGATCATAT GTATAGGCAA 
ACTGAAAATC TCACATCAAA TGGTAGCTCT CCCAAAGTAC ATGGAAACAT AGAAAATAAA 
ATAGAAAAGA ATCCTAGAAT TTGCTGTATT TGTCAGTCCT CATACTTTGT CACTGAAGAT 
TCTGCTTTGG CATGTTATAC GGGGGACAGT AGAAAAACTT GTGTCGGAGA GTCTTCTCTG 
TCCAAAGGCA AAAAATGGCT TAGAGAACAA AGTGATAAGC TTGGAACAAG AAATACTATT 
GAAATCCAAT GTGTAAAGGA ACACACTGAA GATTTTGCAG GAAATGCCTT ATATGAACAT 
AGTTTAGTCA TTATCAGAAC TGAAATTGAT ACAAGTCATG TCTCTGAAAA CCAAGCTTCA 
ACCCTCTTTA GTGACCCTAA TGTGTGTCAC AGCTATCTAT CCCATTCTAG TTTTTGTCAT 
CATGATGATA TGCATAATGA TTCAGGATAT TTCTTAAAAG ATAAAATTGA TTCTGATGTC 
CAGCCAGACA TGAAGAATAC TGAAGGCAAT GCCATTTTCC CTAAAATATC TGCTACAAAA 
GAAATAAAAC TACACCCACA AACTGTAAAT GAAGAGTGTG TTCAAAAACT GGAGACTAAT 
GCTTCACCAT ATGCAAATAA AAATATAGCC ATTGACTCAG CTATGCTGGA TTTAAGGAAT 
TGTAAGGTAG GCTCACCTGT ATTCATTACA ACTCATTCAC AAGAAACTGT AAGAATGAAA 
GAGATATTCA CAGATAACTG TAGTAAAATA GTCGAACAAA ACAGGGAGAG TAAACCAGAC 
ACTTGCCAGA CAAGCTGTCA TAAAGCATTG GATAATTCAG AGGATTTTAT ATGTCCTAGC 
TCTTCAGGTG ATGTCTGCAT AAACTCACCT ATGGCTATTT TTTATCCTCA AAGTGAACAA 
ATTTTACAGC ATAACCAAAG TGTGTCTGGA CTGAAGAAAG CTGCAACACC ACCTGTTAGT 
TTGGAAACTT GGGATACATG TAAATCTATA AGAGGATCTC CTCAGGAAGT CCATCCTTCA 
CGCACTTATG GAATTTTTAG CACAGCAAGT GGAAAAGCTG TACAAGTATC TGATGCTTCA 
TTGGAAAAGG CACGGCAAGT GTTTTCTGAG ATAGATGGTG ATGCTAAACA GTTAGCTTCC 
ATGGTGTCAC TGGAAGGTAA TGAAAAATCA CATCACTCTG TGAAAAGAGA AAGCTCTGTG 
GTGCATAACA CCCATGGTGT ATTGTCACTC CGAAAAACCC TCCCAGGCAA TGTCAGTTCA 
TCTGTATTCT CTGGATTTAG CACCGCAGGT GGAAAACTGG TCACAGTTTC AGAAAGTGCT 
TTACATAAAG TTAAAGGAAT GTTAGAGGAA TTTGATTTGA TCAGAACTGA ACATACTCTC 
CAGCATTCAC CTACACCTGA AGACGTATCA AAAATACCTC CTCAACCTTG TCTTGAAAGC 
AGAACCCCAG AATACTCTGT AAGCTCTAAA TTGCAGAAAA CCTACAATGA TAAATCCAGG 
TCACCAAGTA ATTATAAAGA AAGTGGTTCT TCAGGCAATA CTCAATCTCT TGAAGTTTCT 
CCCCAACTCT CTCAGATGGA GAGAAAGCAA GAAACACAGT CGGTATTAGG AACAAAAGTG 
TCCCAGAGGA AGACTAATAT CTTGGAAAAA AAACAAAACT TACCCCAAAA CATAAAAATA 
GAAAGTAATA AAATGGAAAC ATTTTCTGAT GTTTCCATGA AAACAAATGT AGGAGAGTAC 
TACTCCAAAG AGCCAGAGAA CTATTTTGAA ACAGAAGCAG TGGAAATTGC CAAAGCTTTT 
ATGGAAGATG ATGAGCTGAC GGATTCTGAA CAGACTCATG CCAAATGCTC ACTGTTTGCA 
TGCCCCCAAA ACGAGGCTTT ATTAAATTCG AGAACTAGAA AAAGAGGAGG AATGGCTGGT 
GTTGCAGTTG GACAACCCCC AATTAAAAGG AGCTTATTAA ATGAATTTGA CAGGATAATA 
GAAAGTAAAG GAAAATCCTT AACGCCTTCA AAAAGCACTC CAGATGGCAC AATAAAAGAC 
AGACGATTGT TCACACACCA CATGTCTTTA GAGCCGGTTA CCTGTGGACC CTTCTGCTCA 
AGTAAAGAAA GGCAAGAAAC CCAGAGCCCA CATGTTACCT CACCTGCTCA AGGACTTCAG 
TCTAAGGGGC ATCCTTCAAG ACACTCAGCT GTGGGAAAGT CTTCAAGCAA TCCTACAGTT 
TCTGCCCTAA GATCTGAAAG GACCAGACAC TCAGTCTCAG ACAAATCCAC CAAAGTCTTT 
GTCCCACCTT TCAAGGTGAA ATCACGGTTT CACAGAGATG AACATTTTGA TAGCAAGAAT 
GTTAATTTGG AGGGAAAAAA CCAAAAGAGC GCAGATGGAG TCAGTGAAGA TGGGAACGAC 
AGTGACTTTC CTCAGTTTAA CAAAGATTTA ATGTCAAGCC TTCAGAATGC CAGAGACCTA 
CAGGATATAC GAATTAAAAA CAAAGAAAGG CATCATCTCT GTCCGCAGCC AGGCAGTCTG 
TATCTTACAA AATCATCCAC CCTGCCCCGG ATTTCTCTGC AGGCCGCAGT AGGAGACAGC 
GTTCCTTCTG CCTGTTCTCC TAAGCAGCTC TATATGTATG GCGTTTCTAA AGCATGCATA 
AGCGTTAACA GCAAAAACGC TGAGTATTTT CAGTTTGCCA TTGAGGATCA CTTTGGTAAA 
GAAGCTCTGT GCGCTGGCAA AGGCTTTCGG TTGGCAGATG GTGGATGGCT AATCCCCTCC 
GATGACGGGA AGGCTGGGAA AGAAGAATTT TACAGGGCTC TGTGTGACAC TCCAGGTGTG 
GATCCAAAGC TGATTTCTAG CGTCTGGGTC TCTAACCACT ACAGGTGGAT TGTATGGAAA 
CTGGCAGCTA TGGAATTTGC TTTTCCTAAG GAATTCGCGA ATAGATGTCT AAACCCAGAG 
CGAGTGCTGC TTCAACTAAA ATACAGATAC GATGTGGAAA TTGACAATAG CAGCAGATCA 
GCTCTCAAGA AGATACTGGA AAGGGATGAT ACAGCAGCAA AAACGCTTGT CCTCTGTGTT 
TCTGATATCA TTTCACTAAG CACAAATGTG TCTGAAACTT CAGGCAGTAA AGCTAGCAGT 
GAGGACAGTA ACAAAGTAGA CACGATCGAA CTCACAGATG GGTGGTATGC TGTCAAGGCC 
CAGCTAGACC CTCCACTCCT GGCTCTCGTA AAGAGTGGGA GACTCACTGT GGGTCAGAAG 
ATCATTACTC AGGGAGCAGA GCTGGTGGGC TCTCCCGATG CCTGTGCACC TCTGGAAGCC 
CCAGACTCCC TTAGGCTAAA GATTTCTGCA AACAGCACGC GGCCTGCTCG CTGGCACAGC 
AAGCTGGGGT TCTTTCATGA CCCCAGGCCC TTCCCTCTGC CCTTGTCCTC ACTGTTCAGT 
GATGGAGGAA ATGTTGGTTG TGTGGATGTC ATCGTTCAGA GAGTGTACCC TCTACAGTGG 
GTGGAGAAGA CGGTGTCTGG ATCGTACATA TTTCGTAATG AGAGAGAGGA GGAGAAGGAA 
GCGCTGAGAT TTGCAGAGGC CCAGCAGAAG AAACTAGAGG CCTTGTTCAC CAAAGTCCAC 
ACAGAGCTTA AAGAGCATGA AGAAGACATA GCCCAGCGGC GTGTGCTGTC CCGGGCACTC 
ACACGGCAGC AGGTCCACGC TCTGCAGGAC GGTGCAGAGC TCTATGCAGC AGTGCAGGAT 
GCATCAGACC CAGAGCACCT GGAGACTTGT TTCAGCGAAG AGCAGCTGAG AGCCTTGAAC 
AACTACAGAC AAATGCTGAG CGATAAGAAA CAAGCGCGGA TCCAGTCAGA GTTCCGGAAG 
GCCCTGGAGG CCGCTGAGAA AGAAGAGGGT TTATCAAGGG ACGTCTCAAC TGTGTGGAAG 
CTTCGTGTTA CAAGCTACAA GAAAAGAGAA AAATCAGCTC TGTTGAGTAT CTGGCGTCCA 
TCTTCAGACT TGCCCTCCCT GTTAACAGAA GGACAGAGAT ACAGAATCTA TCATCTTTCG 
GTGTCAAAAT CGAAGAATAA ATTTGAGTGG CCCAGCATCC AGTTAACAGC CACAAAGAGA 
ACTCAGTATC AACAGCTGCC GGTTTCCAGT GAGACCCTGC TCCAGCTTTA CCAGCCCAGG 
GAGCTCCTTC CCTTCAGCAA ACTGTCAGAT CCAGCCTTCC AGCCACCTTG TTCTGAAGTG 
GATGTAGTAG GAGTTGTAGT TTCTGTTGTA AAACCAATAG GTCTTGCTCC TTTGGTCTAC 
TTGTCAGACG AGTGCCTTCA TTTATTAGTG GTGAAATTTG GAATAGACCT TAATGAAGAC 
ATAAAGCCAC GTGTGCTTAT TGCTGCGAGC AACCTCCAGT GGCGGCCGGA GTCCACGTCA 
CGAGTGCCAA CTTTATTTGC TGGGAACTTT TCCGTATTCT CTGCCAGTCC AAAGGAGGCC 
CACTTTCAGG AGAGGGTCAC GAACATGAAG CATGCTATTG AGAACATCGA CACATTTTAC 
AAGGAGGCAG AAAAGAAGCT TATACAGGTG CTGAAGGGAG ACAGTCCAAA GTGGTCCACC 
CCGAACAAAG ACCCCACCCG AGAACCCTAC CCAGCCTCCA CTTGCTCTGC TTCAGACCTT 
GCTTCAGGAG GTCAGTTACC GAGGAGTTCA CCTACTGATC AGCAAAGTTA TCGAAGTCCT 
TTATCATGCT GCACACCGAC GGGGAAATCT ACACCCCTGG CCCACTCAGC CTGGATGGCA 
GCCAAGTCTT GTAGTGGGGA GAATGAGATT GAGGACCCCA AGACCTGTCG AAAAAAAAGG 
GCCTTGGATC TCCTAAGTCG GCTGCCCTTA CCGCCACCGC TCAGTCCTGT CTGCACCTTT 
GTCTCTCCGG CTGCACAGAA GGCCTTTCAG CCACCACGGA GCTGTGGCAC CAAATACCCA 
ACACCTCTAA AAAAAGAGGG ACCCAGTTCC CCTTGGAGCA GGGCGCCATT TCAGAAGGCC 
AGTGGCGTTT CTCTCCTGGA CTGTGATTCA GTAGCTGATG AAGAACTTGC CTTGCTCAGT 
ACCCAAGCCC TTGTGCCTCA CTCAGTGGGA GGAAGTGAAC AAGTGTTTCC CAGTGATTCC 
ACAAGGACAG AAGGACCCTC AGCCAGCACA GAGGCCAGAC CAGCAAATAG ATCCAAGAGG 
GAGTCTCTGA GGGACTGTAG AGATGACAGC GATGGGAAAT TGGCTGCTGA GACAGTTCCA 
GACTACTCAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Brca2 ( XM_017598372.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017453861.1 Rattus norvegicus BRCA2, DNA repair associated (Brca2), transcript variant X1, mRNA.

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CloneID	ORa34515
Clone ID Related Accession (Same CDS sequence)	XM_017598372.1 , NM_031542.2
Accession Version	XM_017598372.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 10032bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	breast cancer type 2 susceptibility protein homolog isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005111.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCGTTG AATACAAAAG GAGACCAACT TTTTGGGAAA TTTTTAAAGC GAGATGCAGC 
ACAGCAGATT TAGGACCAAT AAGCCTCAAT TGGTTTGAAG AACTTTCTTC AGAAGCCCCA 
CCATATAATA CTGAACCTCC AGAAGAATCT GAATATAAAC CCCAAGGCCA TGAACCACAG 
CTATTTAAAA CACCACAGAG GAATCCCTCT TACCATCAGT TTGCTTCAAC TCCAATAATG 
TTCAAAGAAC AAAGTCAAAC TCTACCACTA GACCAGTCAC CTTTCAAAGA ATTAGGGAAT 
GTTGTTGCAA ATAGTAAACG TAAACATCAC AGCAAAAAGA AGGCCAGAAA GGACCCTGTG 
GTAGATGTTG CCAGTCTGCC GCTGAAAGCT TGTCCCAGTG AAAGCCCTTG TACTCCGCGA 
TGCACACAGG TGGCACCGCA GCGAAGAAAG CCAGTGGTAT CTGGAAGTTT ATTCTATACA 
CCAAAACTTG AGGAGACACC AAAACATATT TCTGAAAGTC TGGGAGTTGA AGTGGATCCG 
GATATGTCTT GGACAAGTTC ATTAGCTACA CCACCAACCC TTAGTTCCAC TGTGCTCATA 
GCCCGAGATG AAGAAGCACA CAGAAATGCA TTTCCAGCCG ACTCTCCTGC TAGTTTGAAA 
AGCTATTTTT CTAACCACAA TGAAAGTCTG AAAAAGAATG ATAGATTTAT TCCCTCTGTG 
TCTGACAGTG AAAACAAAAG CCAGCAAGAA GCTTTTAGTC AGGGACTGGA GAAAATGTTA 
GGGGATTCAT CTAGCAAAAT AAATCGCTTC AGAGACTGCC TTAGAAAACC AATACCAAAT 
GTTCTAGAAG ATGGGGAGAC AGCTGTAGAT ACTTCAGGAG AAGATAGTTT CTCGTTATGT 
TTTCCTAAAC GTAGAACCAG AAATCTACAG AAAACGAGAA TGGGCAAGAT GAAGAAGAAA 
ATCTTCAGTG AAACAAGGAC TGATGGATTA AGTGAAGAAG CCAGAGGACA AGCTGATGAT 
AAAAACTCAT TTGCACTTGA AATTGAACCA AGAGATAGTG AGCCCTTAGA TCCATCTGTA 
ACAAACCAGA AGCCCCTTTA CAGCCAGAGT GGGGACATCT CCAGTGAAGC TGGCCAGTGT 
TCAGACAGTA TATGGTCTCA GCCAGATCCC TCTGGTCTAA ATGGAACCCA GACAAGAAAA 
ATACCCCTAC TCCATATTTC TTCTCATAAG CAAAGTATTT TAGAAGACTT CATAGATATG 
AAGAAGGAAG GTACTGGCTC TATTACTTTT CCTCATATTT CTAGTCTTCC AGAACCAGAA 
AAGATGTTCA GTGAGGAAAC TCTGGTAGAC AAGGAACATG AAGGACAGCA TCTTGAGTCA 
CTTGAAGACT CCATCTCAGG GAAACAAATG GTGTCTGGAA CGAGTCAAAC AGCCTGTCTC 
TCTCCGAGTA TCAGGAAGTC TATAGTCAAG ATGAGAGAGC CACTTGAAGA GACTTTGGAT 
ACTGTTTTCT CAGACAGTAT GACCAGCTCA GCCTTTACAG AAGAACTTGA CGCCTCTGCA 
GGGGGACTGG AAATACATAC TGCATGCTCA CAGAGAGAGG ATTCTTTATG TCCTAGTTCA 
GTGGACACTG GAAGCTGGCC AACCACTCTC ACTGACACTT CTGCAACTGT GAAAAATGCA 
GGTTTAATAA CCACTCTCAA AAATAAAAGA AGAAAGTTTA TTTACTCTGT AAGTGATGAT 
GCATCTCATC AAGGAAAAAA ACTACAGACA CAGAGACAGT CAGAGCTTAC TAACCTTTCA 
GCCCCGTTTG AAGCAAGTGC TTTTGAAGTA CCATTCCCCT TTACAAATGT AGATTCAGGT 
ATACCAGATT CTTCAATCAA AAGAAGCAAT TTACCAAATG ATCCTGAAGA GCCATCTTTG 
TCCTTGACCA ACTCTTTTGT GACTGCTGCC AGTAAAGAAA TTAGTTATAT TCATGCATTG 
ATATCTCAGG ATCTAAATGA CAAAGAAGCA ATACTCAGTG AAGAAAAGCC ACAGCCATAT 
ACAGCCCTAG AAGCTGACTT TCTGTCGTGC TTGCCAGAAA GATCATGTGA AAATGATCAA 
AAAAGTCCAA AAGTTTCCGA CCGAAAAGAA AAAGTCTTAG TCTCAGCATG TCGTCCTTCA 
GGAAGGCTTG CAGCAGCAGT GCAGCTCAGC AGCATTAGCT TTGACTCTCA GGAAAACCCT 
CTTGGTAGCC ACAACGTAAC AAGTACTCTT AAATTAACTC CCAGCCCGAA GACACCTCTG 
TCAAAGCCAG TTGTGGTTTC TAGAGGGAAA ATGTGTAAAA TGCCAGAGAA ACTGCAATGT 
AAGAGTTGTA AAGATAATAT TGAATTAAGC AAAAACATCC CTCTGGGGGT TAATGAAATG 
TGTGTCTTAA GTGAAAATTC TGAAACACCT GAGCTTCTGC CACCTCTAGA ATATATAACA 
GAAGTGTCCT CATCAGTGAA GTCACAGTTC AATCAAAATA CAAAAATAGC AGTCGTACAA 
AAGGACCAAA AAGACTCAAC TTTTATTTCA GAAGTAACAG TCCATATGAA TTCTGAAGAA 
CTTTTCCCAG AAAAGGAGAA TAATTTTGCT TTTCAAGTAA CCAATGAAAG CAATAAACCC 
AATATAGGAA GTACTGTGGA ATTCCAGGAA GAAGACCTCA GCCACACAAA AGGGCATAGT 
CTCAAGAACT CTCCCATGAC AGTAGATAGA GACCTAGATG ATGAGCAAGC AGGCCAAGTG 
TTGATTACAG AGGACTCAGA TTCATTAGCA GTAGTCCATG ATTGTACAAA GAAGAGCAGA 
AATACTATAG AGCAGCATCA GAAAGGAACT GCAGACAAAG ACTTCAAGTC AAATTCCTCC 
TTGTATTTGA AATCAGATGG GAACAATGAT TATTTAGACA AATGGTCAGA GTTCTTGGAT 
CCACTCATGA ACCATAAATT TGGAGGTAGC TTCAGAACAG CTTCCAATAA AGAAATAAAA 
CTTTCAGAGG ATAATGTCAA GAAAAGCAAA ATGTTCTTCA AAGATATCGA AGAACAGTAT 
CCTACTAGTT TAGATTGTAT TGACACTGTT AGTACCCTAC AATTAGCAAA CAAGAAGAGA 
CTAAGTGAAC CTCATACATT TGATTTGAAG TCAGGTACTA CTGTATCTAC ACAGTGTCAT 
AGTCAATCAT CTGTTTCTCA TGAAGATACT CACACAGCAC CTCAGATGTT ATCTTCAAAG 
CAAGATTTTC ATTCAAGTCA TAACTTAACG CCCAGCCAAA AAGCAGAAAT TACAGAACTG 
TCTACTATCT TGGAAGAATC AGGAAGTCAG TTTGAATTCA CACAGTTCAA AAATCCAAGC 
CACATAGCAC AGAATAATAC ATCTGCAGTG CTTGGAAACC AGATGGCTGT TGTAAGGACC 
GCTTCTGAGG AGTGGAAAGA TGTTGATCTT CATCTCCCAC TGAATCCCTC CTCTGTAGGT 
CAGATAGATC ACAACAAGAA ATTCGAATGT TTGGTTGGAG TTAAGCAAAG CTCTTCTCAC 
CTGTTAGAAG ACACTTGTAA CCAAAATACA TCTTGTTTTT TACCGATAAA AGAAATGGAG 
TTTGGAGGAT TTTGTTCTGC TCTTGGCACA AAACTTAGTG TGTCTAATGA GGCTCTGAGA 
AAGGCTATGA AACTGTTCAG TGACATTGAA AATATTAGTG AGGAGCCTTC TACAAAAGTA 
GGACCAAGAG GATTCTCTTC ATGTGCACAT CATGATTCTG TTGCTTCCGT GTTTAAGATA 
AAGAAACAAA ACACTGATAA AAGTTTTGAT GAGAAATCTA GTAAGTGCCA GGTAACAGTA 
CAAAATAATA AGGAAATGAC TACCTGTATT CTTGTTGATG AAAATCCTGA AAATTATGTA 
AAGAATATAA AACAAGATAA CAACTATACT GGCTCTCAAA GAAATGCTTA TAAATTAGAA 
AACTCTGATG TTAGTAAATC AAGTACAAGT GGCACAGTTT ATATTAATAA AGGTGACAGT 
GATTTACCTT TTGCTGCTGA AAAAGGCAAT AAGTATCCTG AGTCATGTAC CCAATATGTG 
AGGGAAGAAA ATGCACAAAT TAAGGAAAGT GTATCAGATT TAACATGTTT GGAAGTCATG 
AAAGCTGAGG AAACATGTCA TATGAAATCT TCAGATAAAG AACAATTACC TTCAGATAAG 
ATGGAACAAA ATATGAAAGA GTTTAATATA TCTTTTCAGA CTGCAAGCGG GAAAAATATC 
AGAGTCTCCA AAGAGTCACT AAATAAAAGT GTGAATATTT TAGATCAGGA AACAGAAGAC 
TTGACTGTCA CTTCAGATTC TTTGAATTCT AAAATTCTTT GTGGCATAAA TAAGGACAAA 
ATGCATATTT CATGTCACAA GAAATCAATC AATATTAAAA AGGTATTTGA AGAACATTTC 
CCAATTGGAA CTGTCAGTCA ATTACCAGCT CTTCAGCAGT ATCCTGAATA TGAAATAGAA 
AGTATCAAAG AACCTACTCT GTTGAGTTTT CATACAGCTA GTGGGAAAAA AGTCAAAATT 
ATGCAGGAAT CTTTGGACAA AGTGAAAAAT CTTTTTGATG AGACACAGTA TGTTAGGAAA 
ACCACCAATT TTGGTCATCA AGAATCAAAA CCCCTGAAGG ACAGAGAGGA CTATAAAGAA 
AGACTTACAT TAGCATATGA GAAAATTGAA GTAACTGCCT CAAAATGTGA AGAAATGCAG 
AACTTTGTCT CTAAGCAGAC TGAAATGCTA CCCCAGCAAA ATGATCATAT GTATAGGCAA 
ACTGAAAATC TCACATCAAA TGGTAGCTCT CCCAAAGTAC ATGGAAACAT AGAAAATAAA 
ATAGAAAAGA ATCCTAGAAT TTGCTGTATT TGTCAGTCCT CATACTTTGT CACTGAAGAT 
TCTGCTTTGG CATGTTATAC GGGGGACAGT AGAAAAACTT GTGTCGGAGA GTCTTCTCTG 
TCCAAAGGCA AAAAATGGCT TAGAGAACAA AGTGATAAGC TTGGAACAAG AAATACTATT 
GAAATCCAAT GTGTAAAGGA ACACACTGAA GATTTTGCAG GAAATGCCTT ATATGAACAT 
AGTTTAGTCA TTATCAGAAC TGAAATTGAT ACAAGTCATG TCTCTGAAAA CCAAGCTTCA 
ACCCTCTTTA GTGACCCTAA TGTGTGTCAC AGCTATCTAT CCCATTCTAG TTTTTGTCAT 
CATGATGATA TGCATAATGA TTCAGGATAT TTCTTAAAAG ATAAAATTGA TTCTGATGTC 
CAGCCAGACA TGAAGAATAC TGAAGGCAAT GCCATTTTCC CTAAAATATC TGCTACAAAA 
GAAATAAAAC TACACCCACA AACTGTAAAT GAAGAGTGTG TTCAAAAACT GGAGACTAAT 
GCTTCACCAT ATGCAAATAA AAATATAGCC ATTGACTCAG CTATGCTGGA TTTAAGGAAT 
TGTAAGGTAG GCTCACCTGT ATTCATTACA ACTCATTCAC AAGAAACTGT AAGAATGAAA 
GAGATATTCA CAGATAACTG TAGTAAAATA GTCGAACAAA ACAGGGAGAG TAAACCAGAC 
ACTTGCCAGA CAAGCTGTCA TAAAGCATTG GATAATTCAG AGGATTTTAT ATGTCCTAGC 
TCTTCAGGTG ATGTCTGCAT AAACTCACCT ATGGCTATTT TTTATCCTCA AAGTGAACAA 
ATTTTACAGC ATAACCAAAG TGTGTCTGGA CTGAAGAAAG CTGCAACACC ACCTGTTAGT 
TTGGAAACTT GGGATACATG TAAATCTATA AGAGGATCTC CTCAGGAAGT CCATCCTTCA 
CGCACTTATG GAATTTTTAG CACAGCAAGT GGAAAAGCTG TACAAGTATC TGATGCTTCA 
TTGGAAAAGG CACGGCAAGT GTTTTCTGAG ATAGATGGTG ATGCTAAACA GTTAGCTTCC 
ATGGTGTCAC TGGAAGGTAA TGAAAAATCA CATCACTCTG TGAAAAGAGA AAGCTCTGTG 
GTGCATAACA CCCATGGTGT ATTGTCACTC CGAAAAACCC TCCCAGGCAA TGTCAGTTCA 
TCTGTATTCT CTGGATTTAG CACCGCAGGT GGAAAACTGG TCACAGTTTC AGAAAGTGCT 
TTACATAAAG TTAAAGGAAT GTTAGAGGAA TTTGATTTGA TCAGAACTGA ACATACTCTC 
CAGCATTCAC CTACACCTGA AGACGTATCA AAAATACCTC CTCAACCTTG TCTTGAAAGC 
AGAACCCCAG AATACTCTGT AAGCTCTAAA TTGCAGAAAA CCTACAATGA TAAATCCAGG 
TCACCAAGTA ATTATAAAGA AAGTGGTTCT TCAGGCAATA CTCAATCTCT TGAAGTTTCT 
CCCCAACTCT CTCAGATGGA GAGAAAGCAA GAAACACAGT CGGTATTAGG AACAAAAGTG 
TCCCAGAGGA AGACTAATAT CTTGGAAAAA AAACAAAACT TACCCCAAAA CATAAAAATA 
GAAAGTAATA AAATGGAAAC ATTTTCTGAT GTTTCCATGA AAACAAATGT AGGAGAGTAC 
TACTCCAAAG AGCCAGAGAA CTATTTTGAA ACAGAAGCAG TGGAAATTGC CAAAGCTTTT 
ATGGAAGATG ATGAGCTGAC GGATTCTGAA CAGACTCATG CCAAATGCTC ACTGTTTGCA 
TGCCCCCAAA ACGAGGCTTT ATTAAATTCG AGAACTAGAA AAAGAGGAGG AATGGCTGGT 
GTTGCAGTTG GACAACCCCC AATTAAAAGG AGCTTATTAA ATGAATTTGA CAGGATAATA 
GAAAGTAAAG GAAAATCCTT AACGCCTTCA AAAAGCACTC CAGATGGCAC AATAAAAGAC 
AGACGATTGT TCACACACCA CATGTCTTTA GAGCCGGTTA CCTGTGGACC CTTCTGCTCA 
AGTAAAGAAA GGCAAGAAAC CCAGAGCCCA CATGTTACCT CACCTGCTCA AGGACTTCAG 
TCTAAGGGGC ATCCTTCAAG ACACTCAGCT GTGGGAAAGT CTTCAAGCAA TCCTACAGTT 
TCTGCCCTAA GATCTGAAAG GACCAGACAC TCAGTCTCAG ACAAATCCAC CAAAGTCTTT 
GTCCCACCTT TCAAGGTGAA ATCACGGTTT CACAGAGATG AACATTTTGA TAGCAAGAAT 
GTTAATTTGG AGGGAAAAAA CCAAAAGAGC GCAGATGGAG TCAGTGAAGA TGGGAACGAC 
AGTGACTTTC CTCAGTTTAA CAAAGATTTA ATGTCAAGCC TTCAGAATGC CAGAGACCTA 
CAGGATATAC GAATTAAAAA CAAAGAAAGG CATCATCTCT GTCCGCAGCC AGGCAGTCTG 
TATCTTACAA AATCATCCAC CCTGCCCCGG ATTTCTCTGC AGGCCGCAGT AGGAGACAGC 
GTTCCTTCTG CCTGTTCTCC TAAGCAGCTC TATATGTATG GCGTTTCTAA AGCATGCATA 
AGCGTTAACA GCAAAAACGC TGAGTATTTT CAGTTTGCCA TTGAGGATCA CTTTGGTAAA 
GAAGCTCTGT GCGCTGGCAA AGGCTTTCGG TTGGCAGATG GTGGATGGCT AATCCCCTCC 
GATGACGGGA AGGCTGGGAA AGAAGAATTT TACAGGGCTC TGTGTGACAC TCCAGGTGTG 
GATCCAAAGC TGATTTCTAG CGTCTGGGTC TCTAACCACT ACAGGTGGAT TGTATGGAAA 
CTGGCAGCTA TGGAATTTGC TTTTCCTAAG GAATTCGCGA ATAGATGTCT AAACCCAGAG 
CGAGTGCTGC TTCAACTAAA ATACAGATAC GATGTGGAAA TTGACAATAG CAGCAGATCA 
GCTCTCAAGA AGATACTGGA AAGGGATGAT ACAGCAGCAA AAACGCTTGT CCTCTGTGTT 
TCTGATATCA TTTCACTAAG CACAAATGTG TCTGAAACTT CAGGCAGTAA AGCTAGCAGT 
GAGGACAGTA ACAAAGTAGA CACGATCGAA CTCACAGATG GGTGGTATGC TGTCAAGGCC 
CAGCTAGACC CTCCACTCCT GGCTCTCGTA AAGAGTGGGA GACTCACTGT GGGTCAGAAG 
ATCATTACTC AGGGAGCAGA GCTGGTGGGC TCTCCCGATG CCTGTGCACC TCTGGAAGCC 
CCAGACTCCC TTAGGCTAAA GATTTCTGCA AACAGCACGC GGCCTGCTCG CTGGCACAGC 
AAGCTGGGGT TCTTTCATGA CCCCAGGCCC TTCCCTCTGC CCTTGTCCTC ACTGTTCAGT 
GATGGAGGAA ATGTTGGTTG TGTGGATGTC ATCGTTCAGA GAGTGTACCC TCTACAGTGG 
GTGGAGAAGA CGGTGTCTGG ATCGTACATA TTTCGTAATG AGAGAGAGGA GGAGAAGGAA 
GCGCTGAGAT TTGCAGAGGC CCAGCAGAAG AAACTAGAGG CCTTGTTCAC CAAAGTCCAC 
ACAGAGCTTA AAGAGCATGA AGAAGACATA GCCCAGCGGC GTGTGCTGTC CCGGGCACTC 
ACACGGCAGC AGGTCCACGC TCTGCAGGAC GGTGCAGAGC TCTATGCAGC AGTGCAGGAT 
GCATCAGACC CAGAGCACCT GGAGACTTGT TTCAGCGAAG AGCAGCTGAG AGCCTTGAAC 
AACTACAGAC AAATGCTGAG CGATAAGAAA CAAGCGCGGA TCCAGTCAGA GTTCCGGAAG 
GCCCTGGAGG CCGCTGAGAA AGAAGAGGGT TTATCAAGGG ACGTCTCAAC TGTGTGGAAG 
CTTCGTGTTA CAAGCTACAA GAAAAGAGAA AAATCAGCTC TGTTGAGTAT CTGGCGTCCA 
TCTTCAGACT TGCCCTCCCT GTTAACAGAA GGACAGAGAT ACAGAATCTA TCATCTTTCG 
GTGTCAAAAT CGAAGAATAA ATTTGAGTGG CCCAGCATCC AGTTAACAGC CACAAAGAGA 
ACTCAGTATC AACAGCTGCC GGTTTCCAGT GAGACCCTGC TCCAGCTTTA CCAGCCCAGG 
GAGCTCCTTC CCTTCAGCAA ACTGTCAGAT CCAGCCTTCC AGCCACCTTG TTCTGAAGTG 
GATGTAGTAG GAGTTGTAGT TTCTGTTGTA AAACCAATAG GTCTTGCTCC TTTGGTCTAC 
TTGTCAGACG AGTGCCTTCA TTTATTAGTG GTGAAATTTG GAATAGACCT TAATGAAGAC 
ATAAAGCCAC GTGTGCTTAT TGCTGCGAGC AACCTCCAGT GGCGGCCGGA GTCCACGTCA 
CGAGTGCCAA CTTTATTTGC TGGGAACTTT TCCGTATTCT CTGCCAGTCC AAAGGAGGCC 
CACTTTCAGG AGAGGGTCAC GAACATGAAG CATGCTATTG AGAACATCGA CACATTTTAC 
AAGGAGGCAG AAAAGAAGCT TATACAGGTG CTGAAGGGAG ACAGTCCAAA GTGGTCCACC 
CCGAACAAAG ACCCCACCCG AGAACCCTAC CCAGCCTCCA CTTGCTCTGC TTCAGACCTT 
GCTTCAGGAG GTCAGTTACC GAGGAGTTCA CCTACTGATC AGCAAAGTTA TCGAAGTCCT 
TTATCATGCT GCACACCGAC GGGGAAATCT ACACCCCTGG CCCACTCAGC CTGGATGGCA 
GCCAAGTCTT GTAGTGGGGA GAATGAGATT GAGGACCCCA AGACCTGTCG AAAAAAAAGG 
GCCTTGGATC TCCTAAGTCG GCTGCCCTTA CCGCCACCGC TCAGTCCTGT CTGCACCTTT 
GTCTCTCCGG CTGCACAGAA GGCCTTTCAG CCACCACGGA GCTGTGGCAC CAAATACCCA 
ACACCTCTAA AAAAAGAGGG ACCCAGTTCC CCTTGGAGCA GGGCGCCATT TCAGAAGGCC 
AGTGGCGTTT CTCTCCTGGA CTGTGATTCA GTAGCTGATG AAGAACTTGC CTTGCTCAGT 
ACCCAAGCCC TTGTGCCTCA CTCAGTGGGA GGAAGTGAAC AAGTGTTTCC CAGTGATTCC 
ACAAGGACAG AAGGACCCTC AGCCAGCACA GAGGCCAGAC CAGCAAATAG ATCCAAGAGG 
GAGTCTCTGA GGGACTGTAG AGATGACAGC GATGGGAAAT TGGCTGCTGA GACAGTTCCA 
GACTACTCAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017453861.1
CDS	352..10383
Translation	MTVEYKRRPTFWEIFKARCSTADLGPISLNWFEELSSEAPPYNTEPPEESEYKPQGHEPQLFKTPQRNPSYHQFASTPIMFKEQSQTLPLDQSPFKELGNVVANSKRKHHSKKKARKDPVVDVASLPLKACPSESPCTPRCTQVAPQRRKPVVSGSLFYTPKLEETPKHISESLGVEVDPDMSWTSSLATPPTLSSTVLIARDEEAHRNAFPADSPASLKSYFSNHNESLKKNDRFIPSVSDSENKSQQEAFSQGLEKMLGDSSSKINRFRDCLRKPIPNVLEDGETAVDTSGEDSFSLCFPKRRTRNLQKTRMGKMKKKIFSETRTDGLSEEARGQADDKNSFALEIEPRDSEPLDPSVTNQKPLYSQSGDISSEAGQCSDSIWSQPDPSGLNGTQTRKIPLLHISSHKQSILEDFIDMKKEGTGSITFPHISSLPEPEKMFSEETLVDKEHEGQHLESLEDSISGKQMVSGTSQTACLSPSIRKSIVKMREPLEETLDTVFSDSMTSSAFTEELDASAGGLEIHTACSQREDSLCPSSVDTGSWPTTLTDTSATVKNAGLITTLKNKRRKFIYSVSDDASHQGKKLQTQRQSELTNLSAPFEASAFEVPFPFTNVDSGIPDSSIKRSNLPNDPEEPSLSLTNSFVTAASKEISYIHALISQDLNDKEAILSEEKPQPYTALEADFLSCLPERSCENDQKSPKVSDRKEKVLVSACRPSGRLAAAVQLSSISFDSQENPLGSHNVTSTLKLTPSPKTPLSKPVVVSRGKMCKMPEKLQCKSCKDNIELSKNIPLGVNEMCVLSENSETPELLPPLEYITEVSSSVKSQFNQNTKIAVVQKDQKDSTFISEVTVHMNSEELFPEKENNFAFQVTNESNKPNIGSTVEFQEEDLSHTKGHSLKNSPMTVDRDLDDEQAGQVLITEDSDSLAVVHDCTKKSRNTIEQHQKGTADKDFKSNSSLYLKSDGNNDYLDKWSEFLDPLMNHKFGGSFRTASNKEIKLSEDNVKKSKMFFKDIEEQYPTSLDCIDTVSTLQLANKKRLSEPHTFDLKSGTTVSTQCHSQSSVSHEDTHTAPQMLSSKQDFHSSHNLTPSQKAEITELSTILEESGSQFEFTQFKNPSHIAQNNTSAVLGNQMAVVRTASEEWKDVDLHLPLNPSSVGQIDHNKKFECLVGVKQSSSHLLEDTCNQNTSCFLPIKEMEFGGFCSALGTKLSVSNEALRKAMKLFSDIENISEEPSTKVGPRGFSSCAHHDSVASVFKIKKQNTDKSFDEKSSKCQVTVQNNKEMTTCILVDENPENYVKNIKQDNNYTGSQRNAYKLENSDVSKSSTSGTVYINKGDSDLPFAAEKGNKYPESCTQYVREENAQIKESVSDLTCLEVMKAEETCHMKSSDKEQLPSDKMEQNMKEFNISFQTASGKNIRVSKESLNKSVNILDQETEDLTVTSDSLNSKILCGINKDKMHISCHKKSINIKKVFEEHFPIGTVSQLPALQQYPEYEIESIKEPTLLSFHTASGKKVKIMQESLDKVKNLFDETQYVRKTTNFGHQESKPLKDREDYKERLTLAYEKIEVTASKCEEMQNFVSKQTEMLPQQNDHMYRQTENLTSNGSSPKVHGNIENKIEKNPRICCICQSSYFVTEDSALACYTGDSRKTCVGESSLSKGKKWLREQSDKLGTRNTIEIQCVKEHTEDFAGNALYEHSLVIIRTEIDTSHVSENQASTLFSDPNVCHSYLSHSSFCHHDDMHNDSGYFLKDKIDSDVQPDMKNTEGNAIFPKISATKEIKLHPQTVNEECVQKLETNASPYANKNIAIDSAMLDLRNCKVGSPVFITTHSQETVRMKEIFTDNCSKIVEQNRESKPDTCQTSCHKALDNSEDFICPSSSGDVCINSPMAIFYPQSEQILQHNQSVSGLKKAATPPVSLETWDTCKSIRGSPQEVHPSRTYGIFSTASGKAVQVSDASLEKARQVFSEIDGDAKQLASMVSLEGNEKSHHSVKRESSVVHNTHGVLSLRKTLPGNVSSSVFSGFSTAGGKLVTVSESALHKVKGMLEEFDLIRTEHTLQHSPTPEDVSKIPPQPCLESRTPEYSVSSKLQKTYNDKSRSPSNYKESGSSGNTQSLEVSPQLSQMERKQETQSVLGTKVSQRKTNILEKKQNLPQNIKIESNKMETFSDVSMKTNVGEYYSKEPENYFETEAVEIAKAFMEDDELTDSEQTHAKCSLFACPQNEALLNSRTRKRGGMAGVAVGQPPIKRSLLNEFDRIIESKGKSLTPSKSTPDGTIKDRRLFTHHMSLEPVTCGPFCSSKERQETQSPHVTSPAQGLQSKGHPSRHSAVGKSSSNPTVSALRSERTRHSVSDKSTKVFVPPFKVKSRFHRDEHFDSKNVNLEGKNQKSADGVSEDGNDSDFPQFNKDLMSSLQNARDLQDIRIKNKERHHLCPQPGSLYLTKSSTLPRISLQAAVGDSVPSACSPKQLYMYGVSKACISVNSKNAEYFQFAIEDHFGKEALCAGKGFRLADGGWLIPSDDGKAGKEEFYRALCDTPGVDPKLISSVWVSNHYRWIVWKLAAMEFAFPKEFANRCLNPERVLLQLKYRYDVEIDNSSRSALKKILERDDTAAKTLVLCVSDIISLSTNVSETSGSKASSEDSNKVDTIELTDGWYAVKAQLDPPLLALVKSGRLTVGQKIITQGAELVGSPDACAPLEAPDSLRLKISANSTRPARWHSKLGFFHDPRPFPLPLSSLFSDGGNVGCVDVIVQRVYPLQWVEKTVSGSYIFRNEREEEKEALRFAEAQQKKLEALFTKVHTELKEHEEDIAQRRVLSRALTRQQVHALQDGAELYAAVQDASDPEHLETCFSEEQLRALNNYRQMLSDKKQARIQSEFRKALEAAEKEEGLSRDVSTVWKLRVTSYKKREKSALLSIWRPSSDLPSLLTEGQRYRIYHLSVSKSKNKFEWPSIQLTATKRTQYQQLPVSSETLLQLYQPRELLPFSKLSDPAFQPPCSEVDVVGVVVSVVKPIGLAPLVYLSDECLHLLVVKFGIDLNEDIKPRVLIAASNLQWRPESTSRVPTLFAGNFSVFSASPKEAHFQERVTNMKHAIENIDTFYKEAEKKLIQVLKGDSPKWSTPNKDPTREPYPASTCSASDLASGGQLPRSSPTDQQSYRSPLSCCTPTGKSTPLAHSAWMAAKSCSGENEIEDPKTCRKKRALDLLSRLPLPPPLSPVCTFVSPAAQKAFQPPRSCGTKYPTPLKKEGPSSPWSRAPFQKASGVSLLDCDSVADEELALLSTQALVPHSVGGSEQVFPSDSTRTEGPSASTEARPANRSKRESLRDCRDDSDGKLAAETVPDYS

Target ORF information:

RefSeq Version	XM_017598372.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus BRCA2, DNA repair associated (Brca2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017598372.1

ORF Insert Sequence:

ATGACCGTTG AATACAAAAG GAGACCAACT TTTTGGGAAA TTTTTAAAGC GAGATGCAGC 
ACAGCAGATT TAGGACCAAT AAGCCTCAAT TGGTTTGAAG AACTTTCTTC AGAAGCCCCA 
CCATATAATA CTGAACCTCC AGAAGAATCT GAATATAAAC CCCAAGGCCA TGAACCACAG 
CTATTTAAAA CACCACAGAG GAATCCCTCT TACCATCAGT TTGCTTCAAC TCCAATAATG 
TTCAAAGAAC AAAGTCAAAC TCTACCACTA GACCAGTCAC CTTTCAAAGA ATTAGGGAAT 
GTTGTTGCAA ATAGTAAACG TAAACATCAC AGCAAAAAGA AGGCCAGAAA GGACCCTGTG 
GTAGATGTTG CCAGTCTGCC GCTGAAAGCT TGTCCCAGTG AAAGCCCTTG TACTCCGCGA 
TGCACACAGG TGGCACCGCA GCGAAGAAAG CCAGTGGTAT CTGGAAGTTT ATTCTATACA 
CCAAAACTTG AGGAGACACC AAAACATATT TCTGAAAGTC TGGGAGTTGA AGTGGATCCG 
GATATGTCTT GGACAAGTTC ATTAGCTACA CCACCAACCC TTAGTTCCAC TGTGCTCATA 
GCCCGAGATG AAGAAGCACA CAGAAATGCA TTTCCAGCCG ACTCTCCTGC TAGTTTGAAA 
AGCTATTTTT CTAACCACAA TGAAAGTCTG AAAAAGAATG ATAGATTTAT TCCCTCTGTG 
TCTGACAGTG AAAACAAAAG CCAGCAAGAA GCTTTTAGTC AGGGACTGGA GAAAATGTTA 
GGGGATTCAT CTAGCAAAAT AAATCGCTTC AGAGACTGCC TTAGAAAACC AATACCAAAT 
GTTCTAGAAG ATGGGGAGAC AGCTGTAGAT ACTTCAGGAG AAGATAGTTT CTCGTTATGT 
TTTCCTAAAC GTAGAACCAG AAATCTACAG AAAACGAGAA TGGGCAAGAT GAAGAAGAAA 
ATCTTCAGTG AAACAAGGAC TGATGGATTA AGTGAAGAAG CCAGAGGACA AGCTGATGAT 
AAAAACTCAT TTGCACTTGA AATTGAACCA AGAGATAGTG AGCCCTTAGA TCCATCTGTA 
ACAAACCAGA AGCCCCTTTA CAGCCAGAGT GGGGACATCT CCAGTGAAGC TGGCCAGTGT 
TCAGACAGTA TATGGTCTCA GCCAGATCCC TCTGGTCTAA ATGGAACCCA GACAAGAAAA 
ATACCCCTAC TCCATATTTC TTCTCATAAG CAAAGTATTT TAGAAGACTT CATAGATATG 
AAGAAGGAAG GTACTGGCTC TATTACTTTT CCTCATATTT CTAGTCTTCC AGAACCAGAA 
AAGATGTTCA GTGAGGAAAC TCTGGTAGAC AAGGAACATG AAGGACAGCA TCTTGAGTCA 
CTTGAAGACT CCATCTCAGG GAAACAAATG GTGTCTGGAA CGAGTCAAAC AGCCTGTCTC 
TCTCCGAGTA TCAGGAAGTC TATAGTCAAG ATGAGAGAGC CACTTGAAGA GACTTTGGAT 
ACTGTTTTCT CAGACAGTAT GACCAGCTCA GCCTTTACAG AAGAACTTGA CGCCTCTGCA 
GGGGGACTGG AAATACATAC TGCATGCTCA CAGAGAGAGG ATTCTTTATG TCCTAGTTCA 
GTGGACACTG GAAGCTGGCC AACCACTCTC ACTGACACTT CTGCAACTGT GAAAAATGCA 
GGTTTAATAA CCACTCTCAA AAATAAAAGA AGAAAGTTTA TTTACTCTGT AAGTGATGAT 
GCATCTCATC AAGGAAAAAA ACTACAGACA CAGAGACAGT CAGAGCTTAC TAACCTTTCA 
GCCCCGTTTG AAGCAAGTGC TTTTGAAGTA CCATTCCCCT TTACAAATGT AGATTCAGGT 
ATACCAGATT CTTCAATCAA AAGAAGCAAT TTACCAAATG ATCCTGAAGA GCCATCTTTG 
TCCTTGACCA ACTCTTTTGT GACTGCTGCC AGTAAAGAAA TTAGTTATAT TCATGCATTG 
ATATCTCAGG ATCTAAATGA CAAAGAAGCA ATACTCAGTG AAGAAAAGCC ACAGCCATAT 
ACAGCCCTAG AAGCTGACTT TCTGTCGTGC TTGCCAGAAA GATCATGTGA AAATGATCAA 
AAAAGTCCAA AAGTTTCCGA CCGAAAAGAA AAAGTCTTAG TCTCAGCATG TCGTCCTTCA 
GGAAGGCTTG CAGCAGCAGT GCAGCTCAGC AGCATTAGCT TTGACTCTCA GGAAAACCCT 
CTTGGTAGCC ACAACGTAAC AAGTACTCTT AAATTAACTC CCAGCCCGAA GACACCTCTG 
TCAAAGCCAG TTGTGGTTTC TAGAGGGAAA ATGTGTAAAA TGCCAGAGAA ACTGCAATGT 
AAGAGTTGTA AAGATAATAT TGAATTAAGC AAAAACATCC CTCTGGGGGT TAATGAAATG 
TGTGTCTTAA GTGAAAATTC TGAAACACCT GAGCTTCTGC CACCTCTAGA ATATATAACA 
GAAGTGTCCT CATCAGTGAA GTCACAGTTC AATCAAAATA CAAAAATAGC AGTCGTACAA 
AAGGACCAAA AAGACTCAAC TTTTATTTCA GAAGTAACAG TCCATATGAA TTCTGAAGAA 
CTTTTCCCAG AAAAGGAGAA TAATTTTGCT TTTCAAGTAA CCAATGAAAG CAATAAACCC 
AATATAGGAA GTACTGTGGA ATTCCAGGAA GAAGACCTCA GCCACACAAA AGGGCATAGT 
CTCAAGAACT CTCCCATGAC AGTAGATAGA GACCTAGATG ATGAGCAAGC AGGCCAAGTG 
TTGATTACAG AGGACTCAGA TTCATTAGCA GTAGTCCATG ATTGTACAAA GAAGAGCAGA 
AATACTATAG AGCAGCATCA GAAAGGAACT GCAGACAAAG ACTTCAAGTC AAATTCCTCC 
TTGTATTTGA AATCAGATGG GAACAATGAT TATTTAGACA AATGGTCAGA GTTCTTGGAT 
CCACTCATGA ACCATAAATT TGGAGGTAGC TTCAGAACAG CTTCCAATAA AGAAATAAAA 
CTTTCAGAGG ATAATGTCAA GAAAAGCAAA ATGTTCTTCA AAGATATCGA AGAACAGTAT 
CCTACTAGTT TAGATTGTAT TGACACTGTT AGTACCCTAC AATTAGCAAA CAAGAAGAGA 
CTAAGTGAAC CTCATACATT TGATTTGAAG TCAGGTACTA CTGTATCTAC ACAGTGTCAT 
AGTCAATCAT CTGTTTCTCA TGAAGATACT CACACAGCAC CTCAGATGTT ATCTTCAAAG 
CAAGATTTTC ATTCAAGTCA TAACTTAACG CCCAGCCAAA AAGCAGAAAT TACAGAACTG 
TCTACTATCT TGGAAGAATC AGGAAGTCAG TTTGAATTCA CACAGTTCAA AAATCCAAGC 
CACATAGCAC AGAATAATAC ATCTGCAGTG CTTGGAAACC AGATGGCTGT TGTAAGGACC 
GCTTCTGAGG AGTGGAAAGA TGTTGATCTT CATCTCCCAC TGAATCCCTC CTCTGTAGGT 
CAGATAGATC ACAACAAGAA ATTCGAATGT TTGGTTGGAG TTAAGCAAAG CTCTTCTCAC 
CTGTTAGAAG ACACTTGTAA CCAAAATACA TCTTGTTTTT TACCGATAAA AGAAATGGAG 
TTTGGAGGAT TTTGTTCTGC TCTTGGCACA AAACTTAGTG TGTCTAATGA GGCTCTGAGA 
AAGGCTATGA AACTGTTCAG TGACATTGAA AATATTAGTG AGGAGCCTTC TACAAAAGTA 
GGACCAAGAG GATTCTCTTC ATGTGCACAT CATGATTCTG TTGCTTCCGT GTTTAAGATA 
AAGAAACAAA ACACTGATAA AAGTTTTGAT GAGAAATCTA GTAAGTGCCA GGTAACAGTA 
CAAAATAATA AGGAAATGAC TACCTGTATT CTTGTTGATG AAAATCCTGA AAATTATGTA 
AAGAATATAA AACAAGATAA CAACTATACT GGCTCTCAAA GAAATGCTTA TAAATTAGAA 
AACTCTGATG TTAGTAAATC AAGTACAAGT GGCACAGTTT ATATTAATAA AGGTGACAGT 
GATTTACCTT TTGCTGCTGA AAAAGGCAAT AAGTATCCTG AGTCATGTAC CCAATATGTG 
AGGGAAGAAA ATGCACAAAT TAAGGAAAGT GTATCAGATT TAACATGTTT GGAAGTCATG 
AAAGCTGAGG AAACATGTCA TATGAAATCT TCAGATAAAG AACAATTACC TTCAGATAAG 
ATGGAACAAA ATATGAAAGA GTTTAATATA TCTTTTCAGA CTGCAAGCGG GAAAAATATC 
AGAGTCTCCA AAGAGTCACT AAATAAAAGT GTGAATATTT TAGATCAGGA AACAGAAGAC 
TTGACTGTCA CTTCAGATTC TTTGAATTCT AAAATTCTTT GTGGCATAAA TAAGGACAAA 
ATGCATATTT CATGTCACAA GAAATCAATC AATATTAAAA AGGTATTTGA AGAACATTTC 
CCAATTGGAA CTGTCAGTCA ATTACCAGCT CTTCAGCAGT ATCCTGAATA TGAAATAGAA 
AGTATCAAAG AACCTACTCT GTTGAGTTTT CATACAGCTA GTGGGAAAAA AGTCAAAATT 
ATGCAGGAAT CTTTGGACAA AGTGAAAAAT CTTTTTGATG AGACACAGTA TGTTAGGAAA 
ACCACCAATT TTGGTCATCA AGAATCAAAA CCCCTGAAGG ACAGAGAGGA CTATAAAGAA 
AGACTTACAT TAGCATATGA GAAAATTGAA GTAACTGCCT CAAAATGTGA AGAAATGCAG 
AACTTTGTCT CTAAGCAGAC TGAAATGCTA CCCCAGCAAA ATGATCATAT GTATAGGCAA 
ACTGAAAATC TCACATCAAA TGGTAGCTCT CCCAAAGTAC ATGGAAACAT AGAAAATAAA 
ATAGAAAAGA ATCCTAGAAT TTGCTGTATT TGTCAGTCCT CATACTTTGT CACTGAAGAT 
TCTGCTTTGG CATGTTATAC GGGGGACAGT AGAAAAACTT GTGTCGGAGA GTCTTCTCTG 
TCCAAAGGCA AAAAATGGCT TAGAGAACAA AGTGATAAGC TTGGAACAAG AAATACTATT 
GAAATCCAAT GTGTAAAGGA ACACACTGAA GATTTTGCAG GAAATGCCTT ATATGAACAT 
AGTTTAGTCA TTATCAGAAC TGAAATTGAT ACAAGTCATG TCTCTGAAAA CCAAGCTTCA 
ACCCTCTTTA GTGACCCTAA TGTGTGTCAC AGCTATCTAT CCCATTCTAG TTTTTGTCAT 
CATGATGATA TGCATAATGA TTCAGGATAT TTCTTAAAAG ATAAAATTGA TTCTGATGTC 
CAGCCAGACA TGAAGAATAC TGAAGGCAAT GCCATTTTCC CTAAAATATC TGCTACAAAA 
GAAATAAAAC TACACCCACA AACTGTAAAT GAAGAGTGTG TTCAAAAACT GGAGACTAAT 
GCTTCACCAT ATGCAAATAA AAATATAGCC ATTGACTCAG CTATGCTGGA TTTAAGGAAT 
TGTAAGGTAG GCTCACCTGT ATTCATTACA ACTCATTCAC AAGAAACTGT AAGAATGAAA 
GAGATATTCA CAGATAACTG TAGTAAAATA GTCGAACAAA ACAGGGAGAG TAAACCAGAC 
ACTTGCCAGA CAAGCTGTCA TAAAGCATTG GATAATTCAG AGGATTTTAT ATGTCCTAGC 
TCTTCAGGTG ATGTCTGCAT AAACTCACCT ATGGCTATTT TTTATCCTCA AAGTGAACAA 
ATTTTACAGC ATAACCAAAG TGTGTCTGGA CTGAAGAAAG CTGCAACACC ACCTGTTAGT 
TTGGAAACTT GGGATACATG TAAATCTATA AGAGGATCTC CTCAGGAAGT CCATCCTTCA 
CGCACTTATG GAATTTTTAG CACAGCAAGT GGAAAAGCTG TACAAGTATC TGATGCTTCA 
TTGGAAAAGG CACGGCAAGT GTTTTCTGAG ATAGATGGTG ATGCTAAACA GTTAGCTTCC 
ATGGTGTCAC TGGAAGGTAA TGAAAAATCA CATCACTCTG TGAAAAGAGA AAGCTCTGTG 
GTGCATAACA CCCATGGTGT ATTGTCACTC CGAAAAACCC TCCCAGGCAA TGTCAGTTCA 
TCTGTATTCT CTGGATTTAG CACCGCAGGT GGAAAACTGG TCACAGTTTC AGAAAGTGCT 
TTACATAAAG TTAAAGGAAT GTTAGAGGAA TTTGATTTGA TCAGAACTGA ACATACTCTC 
CAGCATTCAC CTACACCTGA AGACGTATCA AAAATACCTC CTCAACCTTG TCTTGAAAGC 
AGAACCCCAG AATACTCTGT AAGCTCTAAA TTGCAGAAAA CCTACAATGA TAAATCCAGG 
TCACCAAGTA ATTATAAAGA AAGTGGTTCT TCAGGCAATA CTCAATCTCT TGAAGTTTCT 
CCCCAACTCT CTCAGATGGA GAGAAAGCAA GAAACACAGT CGGTATTAGG AACAAAAGTG 
TCCCAGAGGA AGACTAATAT CTTGGAAAAA AAACAAAACT TACCCCAAAA CATAAAAATA 
GAAAGTAATA AAATGGAAAC ATTTTCTGAT GTTTCCATGA AAACAAATGT AGGAGAGTAC 
TACTCCAAAG AGCCAGAGAA CTATTTTGAA ACAGAAGCAG TGGAAATTGC CAAAGCTTTT 
ATGGAAGATG ATGAGCTGAC GGATTCTGAA CAGACTCATG CCAAATGCTC ACTGTTTGCA 
TGCCCCCAAA ACGAGGCTTT ATTAAATTCG AGAACTAGAA AAAGAGGAGG AATGGCTGGT 
GTTGCAGTTG GACAACCCCC AATTAAAAGG AGCTTATTAA ATGAATTTGA CAGGATAATA 
GAAAGTAAAG GAAAATCCTT AACGCCTTCA AAAAGCACTC CAGATGGCAC AATAAAAGAC 
AGACGATTGT TCACACACCA CATGTCTTTA GAGCCGGTTA CCTGTGGACC CTTCTGCTCA 
AGTAAAGAAA GGCAAGAAAC CCAGAGCCCA CATGTTACCT CACCTGCTCA AGGACTTCAG 
TCTAAGGGGC ATCCTTCAAG ACACTCAGCT GTGGGAAAGT CTTCAAGCAA TCCTACAGTT 
TCTGCCCTAA GATCTGAAAG GACCAGACAC TCAGTCTCAG ACAAATCCAC CAAAGTCTTT 
GTCCCACCTT TCAAGGTGAA ATCACGGTTT CACAGAGATG AACATTTTGA TAGCAAGAAT 
GTTAATTTGG AGGGAAAAAA CCAAAAGAGC GCAGATGGAG TCAGTGAAGA TGGGAACGAC 
AGTGACTTTC CTCAGTTTAA CAAAGATTTA ATGTCAAGCC TTCAGAATGC CAGAGACCTA 
CAGGATATAC GAATTAAAAA CAAAGAAAGG CATCATCTCT GTCCGCAGCC AGGCAGTCTG 
TATCTTACAA AATCATCCAC CCTGCCCCGG ATTTCTCTGC AGGCCGCAGT AGGAGACAGC 
GTTCCTTCTG CCTGTTCTCC TAAGCAGCTC TATATGTATG GCGTTTCTAA AGCATGCATA 
AGCGTTAACA GCAAAAACGC TGAGTATTTT CAGTTTGCCA TTGAGGATCA CTTTGGTAAA 
GAAGCTCTGT GCGCTGGCAA AGGCTTTCGG TTGGCAGATG GTGGATGGCT AATCCCCTCC 
GATGACGGGA AGGCTGGGAA AGAAGAATTT TACAGGGCTC TGTGTGACAC TCCAGGTGTG 
GATCCAAAGC TGATTTCTAG CGTCTGGGTC TCTAACCACT ACAGGTGGAT TGTATGGAAA 
CTGGCAGCTA TGGAATTTGC TTTTCCTAAG GAATTCGCGA ATAGATGTCT AAACCCAGAG 
CGAGTGCTGC TTCAACTAAA ATACAGATAC GATGTGGAAA TTGACAATAG CAGCAGATCA 
GCTCTCAAGA AGATACTGGA AAGGGATGAT ACAGCAGCAA AAACGCTTGT CCTCTGTGTT 
TCTGATATCA TTTCACTAAG CACAAATGTG TCTGAAACTT CAGGCAGTAA AGCTAGCAGT 
GAGGACAGTA ACAAAGTAGA CACGATCGAA CTCACAGATG GGTGGTATGC TGTCAAGGCC 
CAGCTAGACC CTCCACTCCT GGCTCTCGTA AAGAGTGGGA GACTCACTGT GGGTCAGAAG 
ATCATTACTC AGGGAGCAGA GCTGGTGGGC TCTCCCGATG CCTGTGCACC TCTGGAAGCC 
CCAGACTCCC TTAGGCTAAA GATTTCTGCA AACAGCACGC GGCCTGCTCG CTGGCACAGC 
AAGCTGGGGT TCTTTCATGA CCCCAGGCCC TTCCCTCTGC CCTTGTCCTC ACTGTTCAGT 
GATGGAGGAA ATGTTGGTTG TGTGGATGTC ATCGTTCAGA GAGTGTACCC TCTACAGTGG 
GTGGAGAAGA CGGTGTCTGG ATCGTACATA TTTCGTAATG AGAGAGAGGA GGAGAAGGAA 
GCGCTGAGAT TTGCAGAGGC CCAGCAGAAG AAACTAGAGG CCTTGTTCAC CAAAGTCCAC 
ACAGAGCTTA AAGAGCATGA AGAAGACATA GCCCAGCGGC GTGTGCTGTC CCGGGCACTC 
ACACGGCAGC AGGTCCACGC TCTGCAGGAC GGTGCAGAGC TCTATGCAGC AGTGCAGGAT 
GCATCAGACC CAGAGCACCT GGAGACTTGT TTCAGCGAAG AGCAGCTGAG AGCCTTGAAC 
AACTACAGAC AAATGCTGAG CGATAAGAAA CAAGCGCGGA TCCAGTCAGA GTTCCGGAAG 
GCCCTGGAGG CCGCTGAGAA AGAAGAGGGT TTATCAAGGG ACGTCTCAAC TGTGTGGAAG 
CTTCGTGTTA CAAGCTACAA GAAAAGAGAA AAATCAGCTC TGTTGAGTAT CTGGCGTCCA 
TCTTCAGACT TGCCCTCCCT GTTAACAGAA GGACAGAGAT ACAGAATCTA TCATCTTTCG 
GTGTCAAAAT CGAAGAATAA ATTTGAGTGG CCCAGCATCC AGTTAACAGC CACAAAGAGA 
ACTCAGTATC AACAGCTGCC GGTTTCCAGT GAGACCCTGC TCCAGCTTTA CCAGCCCAGG 
GAGCTCCTTC CCTTCAGCAA ACTGTCAGAT CCAGCCTTCC AGCCACCTTG TTCTGAAGTG 
GATGTAGTAG GAGTTGTAGT TTCTGTTGTA AAACCAATAG GTCTTGCTCC TTTGGTCTAC 
TTGTCAGACG AGTGCCTTCA TTTATTAGTG GTGAAATTTG GAATAGACCT TAATGAAGAC 
ATAAAGCCAC GTGTGCTTAT TGCTGCGAGC AACCTCCAGT GGCGGCCGGA GTCCACGTCA 
CGAGTGCCAA CTTTATTTGC TGGGAACTTT TCCGTATTCT CTGCCAGTCC AAAGGAGGCC 
CACTTTCAGG AGAGGGTCAC GAACATGAAG CATGCTATTG AGAACATCGA CACATTTTAC 
AAGGAGGCAG AAAAGAAGCT TATACAGGTG CTGAAGGGAG ACAGTCCAAA GTGGTCCACC 
CCGAACAAAG ACCCCACCCG AGAACCCTAC CCAGCCTCCA CTTGCTCTGC TTCAGACCTT 
GCTTCAGGAG GTCAGTTACC GAGGAGTTCA CCTACTGATC AGCAAAGTTA TCGAAGTCCT 
TTATCATGCT GCACACCGAC GGGGAAATCT ACACCCCTGG CCCACTCAGC CTGGATGGCA 
GCCAAGTCTT GTAGTGGGGA GAATGAGATT GAGGACCCCA AGACCTGTCG AAAAAAAAGG 
GCCTTGGATC TCCTAAGTCG GCTGCCCTTA CCGCCACCGC TCAGTCCTGT CTGCACCTTT 
GTCTCTCCGG CTGCACAGAA GGCCTTTCAG CCACCACGGA GCTGTGGCAC CAAATACCCA 
ACACCTCTAA AAAAAGAGGG ACCCAGTTCC CCTTGGAGCA GGGCGCCATT TCAGAAGGCC 
AGTGGCGTTT CTCTCCTGGA CTGTGATTCA GTAGCTGATG AAGAACTTGC CTTGCTCAGT 
ACCCAAGCCC TTGTGCCTCA CTCAGTGGGA GGAAGTGAAC AAGTGTTTCC CAGTGATTCC 
ACAAGGACAG AAGGACCCTC AGCCAGCACA GAGGCCAGAC CAGCAAATAG ATCCAAGAGG 
GAGTCTCTGA GGGACTGTAG AGATGACAGC GATGGGAAAT TGGCTGCTGA GACAGTTCCA 
GACTACTCAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Brca2 ( XM_017598373.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017453862.1 Rattus norvegicus BRCA2, DNA repair associated (Brca2), transcript variant X2, mRNA.

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CloneID	ORa57845
Clone ID Related Accession (Same CDS sequence)	XM_017598373.1
Accession Version	XM_017598373.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9447bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	breast cancer type 2 susceptibility protein homolog isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005111.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCGTTG AATACAAAAG GAGACCAACT TTTTGGGAAA TTTTTAAAGC GAGATGCAGC 
ACAGCAGATT TAGGACCAAT AAGCCTCAAT TGGTTTGAAG AACTTTCTTC AGAAGCCCCA 
CCATATAATA CTGAACCTCC AGAAGAATCT GAATATAAAC CCCAAGGCCA TGAACCACAG 
CTATTTAAAA CACCACAGAG GAATCCCTCT TACCATCAGT TTGCTTCAAC TCCAATAATG 
TTCAAAGAAC AAAGTCAAAC TCTACCACTA GACCAGTCAC CTTTCAAAGA ATTAGGGAAT 
GTTGTTGCAA ATAGTAAACG TAAACATCAC AGCAAAAAGA AGGCCAGAAA GGACCCTGTG 
GTAGATGTTG CCAGTCTGCC GCTGAAAGCT TGTCCCAGTG AAAGCCCTTG TACTCCGCGA 
TGCACACAGG TGGCACCGCA GCGAAGAAAG CCAGTGGTAT CTGGAAGTTT ATTCTATACA 
CCAAAACTTG AGGAGACACC AAAACATATT TCTGAAAGTC TGGGAGTTGA AGTGGATCCG 
GATATGTCTT GGACAAGTTC ATTAGCTACA CCACCAACCC TTAGTTCCAC TGTGCTCATA 
GCCCGAGATG AAGAAGCACA CAGAAATGCA TTTCCAGCCG ACTCTCCTGC TAGTTTGAAA 
AGCTATTTTT CTAACCACAA TGAAAGTCTG AAAAAGAATG ATAGATTTAT TCCCTCTGTG 
TCTGACAGTG AAAACAAAAG CCAGCAAGAA GCTTTTAGTC AGGGACTGGA GAAAATGTTA 
GGGGATTCAT CTAGCAAAAT AAATCGCTTC AGAGACTGCC TTAGAAAACC AATACCAAAT 
GTTCTAGAAG ATGGGGAGAC AGCTGTAGAT ACTTCAGGAG AAGATAGTTT CTCGTTATGT 
TTTCCTAAAC GTAGAACCAG AAATCTACAG AAAACGAGAA TGGGCAAGAT GAAGAAGAAA 
ATCTTCAGTG AAACAAGGAC TGATGGATTA AGTGAAGAAG CCAGAGGACA AGCTGATGAT 
AAAAACTCAT TTGCACTTGA AATTGAACCA AGAGATAGTG AGCCCTTAGA TCCATCTGTA 
ACAAACCAGA AGCCCCTTTA CAGCCAGAGT GGGGACATCT CCAGTGAAGC TGGCCAGTGT 
TCAGACAGTA TATGGTCTCA GCCAGATCCC TCTGGTCTAA ATGGAACCCA GACAAGAAAA 
ATACCCCTAC TCCATATTTC TTCTCATAAG CAAAGTATTT TAGAAGACTT CATAGATATG 
AAGAAGGAAG GTACTGGCTC TATTACTTTT CCTCATATTT CTAGTCTTCC AGAACCAGAA 
AAGATGTTCA GTGAGGAAAC TCTGGTAGAC AAGGAACATG AAGGACAGCA TCTTGAGTCA 
CTTGAAGACT CCATCTCAGG GAAACAAATG GTGTCTGGAA CGAGTCAAAC AGCCTGTCTC 
TCTCCGAGTA TCAGGAAGTC TATAGTCAAG ATGAGAGAGC CACTTGAAGA GACTTTGGAT 
ACTGTTTTCT CAGACAGTAT GACCAGCTCA GCCTTTACAG AAGAACTTGA CGCCTCTGCA 
GGGGGACTGG AAATACATAC TGCATGCTCA CAGAGAGAGG ATTCTTTATG TCCTAGTTCA 
GTGGACACTG GAAGCTGGCC AACCACTCTC ACTGACACTT CTGCAACTGT GAAAAATGCA 
GGTTTAATAA CCACTCTCAA AAATAAAAGA AGAAAGTTTA TTTACTCTGT AAGTGATGAT 
GCATCTCATC AAGGAAAAAA ACTACAGACA CAGAGACAGT CAGAGCTTAC TAACCTTTCA 
GCCCCGTTTG AAGCAAGTGC TTTTGAAGTA CCATTCCCCT TTACAAATGT AGATTCAGGT 
ATACCAGATT CTTCAATCAA AAGAAGCAAT TTACCAAATG ATCCTGAAGA GCCATCTTTG 
TCCTTGACCA ACTCTTTTGT GACTGCTGCC AGTAAAGAAA TTAGTTATAT TCATGCATTG 
ATATCTCAGG ATCTAAATGA CAAAGAAGCA ATACTCAGTG AAGAAAAGCC ACAGCCATAT 
ACAGCCCTAG AAGCTGACTT TCTGTCGTGC TTGCCAGAAA GATCATGTGA AAATGATCAA 
AAAAGTCCAA AAGTTTCCGA CCGAAAAGAA AAAGTCTTAG TCTCAGCATG TCGTCCTTCA 
GGAAGGCTTG CAGCAGCAGT GCAGCTCAGC AGCATTAGCT TTGACTCTCA GGAAAACCCT 
CTTGGTAGCC ACAACGTAAC AAGTACTCTT AAATTAACTC CCAGCCCGAA GACACCTCTG 
TCAAAGCCAG TTGTGGTTTC TAGAGGGAAA ATGTGTAAAA TGCCAGAGAA ACTGCAATGT 
AAGAGTTGTA AAGATAATAT TGAATTAAGC AAAAACATCC CTCTGGGGGT TAATGAAATG 
TGTGTCTTAA GTGAAAATTC TGAAACACCT GAGCTTCTGC CACCTCTAGA ATATATAACA 
GAAGTGTCCT CATCAGTGAA GTCACAGTTC AATCAAAATA CAAAAATAGC AGTCGTACAA 
AAGGACCAAA AAGACTCAAC TTTTATTTCA GAAGTAACAG TCCATATGAA TTCTGAAGAA 
CTTTTCCCAG AAAAGGAGAA TAATTTTGCT TTTCAAGTAA CCAATGAAAG CAATAAACCC 
AATATAGGAA GTACTGTGGA ATTCCAGGAA GAAGACCTCA GCCACACAAA AGGGCATAGT 
CTCAAGAACT CTCCCATGAC AGTAGATAGA GACCTAGATG ATGAGCAAGC AGGCCAAGTG 
TTGATTACAG AGGACTCAGA TTCATTAGCA GTAGTCCATG ATTGTACAAA GAAGAGCAGA 
AATACTATAG AGCAGCATCA GAAAGGAACT GCAGACAAAG ACTTCAAGTC AAATTCCTCC 
TTGTATTTGA AATCAGATGG GAACAATGAT TATTTAGACA AATGGTCAGA GTTCTTGGAT 
CCACTCATGA ACCATAAATT TGGAGGTAGC TTCAGAACAG CTTCCAATAA AGAAATAAAA 
CTTTCAGAGG ATAATGTCAA GAAAAGCAAA ATGTTCTTCA AAGATATCGA AGAACAGTAT 
CCTACTAGTT TAGATTGTAT TGACACTGTT AGTACCCTAC AATTAGCAAA CAAGAAGAGA 
CTAAGTGAAC CTCATACATT TGATTTGAAG TCAGGTACTA CTGTATCTAC ACAGTGTCAT 
AGTCAATCAT CTGTTTCTCA TGAAGATACT CACACAGCAC CTCAGATGTT ATCTTCAAAG 
CAAGATTTTC ATTCAAGTCA TAACTTAACG CCCAGCCAAA AAGCAGAAAT TACAGAACTG 
TCTACTATCT TGGAAGAATC AGGAAGTCAG TTTGAATTCA CACAGTTCAA AAATCCAAGC 
CACATAGCAC AGAATAATAC ATCTGCAGTG CTTGGAAACC AGATGGCTGT TGTAAGGACC 
GCTTCTGAGG AGTGGAAAGA TGTTGATCTT CATCTCCCAC TGAATCCCTC CTCTGTAGGT 
CAGATAGATC ACAACAAGAA ATTCGAATGT TTGGTTGGAG TTAAGCAAAG CTCTTCTCAC 
CTGTTAGAAG ACACTTGTAA CCAAAATACA TCTTGTTTTT TACCGATAAA AGAAATGGAG 
TTTGGAGGAT TTTGTTCTGC TCTTGGCACA AAACTTAGTG TGTCTAATGA GGCTCTGAGA 
AAGGCTATGA AACTGTTCAG TGACATTGAA AATATTAGTG AGGAGCCTTC TACAAAAGTA 
GGACCAAGAG GATTCTCTTC ATGTGCACAT CATGATTCTG TTGCTTCCGT GTTTAAGATA 
AAGAAACAAA ACACTGATAA AAGTTTTGAT GAGAAATCTA GTAAGTGCCA GGTAACAGTA 
CAAAATAATA AGGAAATGAC TACCTGTATT CTTGTTGATG AAAATCCTGA AAATTATGTA 
AAGAATATAA AACAAGATAA CAACTATACT GGCTCTCAAA GAAATGCTTA TAAATTAGAA 
AACTCTGATG TTAGTAAATC AAGTACAAGT GGCACAGTTT ATATTAATAA AGGTGACAGT 
GATTTACCTT TTGCTGCTGA AAAAGGCAAT AAGTATCCTG AGTCATGTAC CCAATATGTG 
AGGGAAGAAA ATGCACAAAT TAAGGAAAGT GTATCAGATT TAACATGTTT GGAAGTCATG 
AAAGCTGAGG AAACATGTCA TATGAAATCT TCAGATAAAG AACAATTACC TTCAGATAAG 
ATGGAACAAA ATATGAAAGA GTTTAATATA TCTTTTCAGA CTGCAAGCGG GAAAAATATC 
AGAGTCTCCA AAGAGTCACT AAATAAAAGT GTGAATATTT TAGATCAGGA AACAGAAGAC 
TTGACTGTCA CTTCAGATTC TTTGAATTCT AAAATTCTTT GTGGCATAAA TAAGGACAAA 
ATGCATATTT CATGTCACAA GAAATCAATC AATATTAAAA AGGTATTTGA AGAACATTTC 
CCAATTGGAA CTGTCAGTCA ATTACCAGCT CTTCAGCAGT ATCCTGAATA TGAAATAGAA 
AGTATCAAAG AACCTACTCT GTTGAGTTTT CATACAGCTA GTGGGAAAAA AGTCAAAATT 
ATGCAGGAAT CTTTGGACAA AGTGAAAAAT CTTTTTGATG AGACACAGTA TGTTAGGAAA 
ACCACCAATT TTGGTCATCA AGAATCAAAA CCCCTGAAGG ACAGAGAGGA CTATAAAGAA 
AGACTTACAT TAGCATATGA GAAAATTGAA GTAACTGCCT CAAAATGTGA AGAAATGCAG 
AACTTTGTCT CTAAGCAGAC TGAAATGCTA CCCCAGCAAA ATGATCATAT GTATAGGCAA 
ACTGAAAATC TCACATCAAA TGGTAGCTCT CCCAAAGTAC ATGGAAACAT AGAAAATAAA 
ATAGAAAAGA ATCCTAGAAT TTGCTGTATT TGTCAGTCCT CATACTTTGT CACTGAAGAT 
TCTGCTTTGG CATGTTATAC GGGGGACAGT AGAAAAACTT GTGTCGGAGA GTCTTCTCTG 
TCCAAAGGCA AAAAATGGCT TAGAGAACAA AGTGATAAGC TTGGAACAAG AAATACTATT 
GAAATCCAAT GTGTAAAGGA ACACACTGAA GATTTTGCAG GAAATGCCTT ATATGAACAT 
AGTTTAGTCA TTATCAGAAC TGAAATTGAT ACAAGTCATG TCTCTGAAAA CCAAGCTTCA 
ACCCTCTTTA GTGACCCTAA TGTGTGTCAC AGCTATCTAT CCCATTCTAG TTTTTGTCAT 
CATGATGATA TGCATAATGA TTCAGGATAT TTCTTAAAAG ATAAAATTGA TTCTGATGTC 
CAGCCAGACA TGAAGAATAC TGAAGGCAAT GCCATTTTCC CTAAAATATC TGCTACAAAA 
GAAATAAAAC TACACCCACA AACTGTAAAT GAAGAGTGTG TTCAAAAACT GGAGACTAAT 
GCTTCACCAT ATGCAAATAA AAATATAGCC ATTGACTCAG CTATGCTGGA TTTAAGGAAT 
TGTAAGGTAG GCTCACCTGT ATTCATTACA ACTCATTCAC AAGAAACTGT AAGAATGAAA 
GAGATATTCA CAGATAACTG TAGTAAAATA GTCGAACAAA ACAGGGAGAG TAAACCAGAC 
ACTTGCCAGA CAAGCTGTCA TAAAGCATTG GATAATTCAG AGGATTTTAT ATGTCCTAGC 
TCTTCAGGTG ATGTCTGCAT AAACTCACCT ATGGCTATTT TTTATCCTCA AAGTGAACAA 
ATTTTACAGC ATAACCAAAG TGTGTCTGGA CTGAAGAAAG CTGCAACACC ACCTGTTAGT 
TTGGAAACTT GGGATACATG TAAATCTATA AGAGGATCTC CTCAGGAAGT CCATCCTTCA 
CGCACTTATG GAATTTTTAG CACAGCAAGT GGAAAAGCTG TACAAGTATC TGATGCTTCA 
TTGGAAAAGG CACGGCAAGT GTTTTCTGAG ATAGATGGTG ATGCTAAACA GTTAGCTTCC 
ATGGTGTCAC TGGAAGGTAA TGAAAAATCA CATCACTCTG TGAAAAGAGA AAGCTCTGTG 
GTGCATAACA CCCATGGTGT ATTGTCACTC CGAAAAACCC TCCCAGGCAA TGTCAGTTCA 
TCTGTATTCT CTGGATTTAG CACCGCAGGT GGAAAACTGG TCACAGTTTC AGAAAGTGCT 
TTACATAAAG TTAAAGGAAT GTTAGAGGAA TTTGATTTGA TCAGAACTGA ACATACTCTC 
CAGCATTCAC CTACACCTGA AGACGTATCA AAAATACCTC CTCAACCTTG TCTTGAAAGC 
AGAACCCCAG AATACTCTGT AAGCTCTAAA TTGCAGAAAA CCTACAATGA TAAATCCAGG 
TCACCAAGTA ATTATAAAGA AAGTGGTTCT TCAGGCAATA CTCAATCTCT TGAAGTTTCT 
CCCCAACTCT CTCAGATGGA GAGAAAGCAA GAAACACAGT CGGTATTAGG AACAAAAGTG 
TCCCAGAGGA AGACTAATAT CTTGGAAAAA AAACAAAACT TACCCCAAAA CATAAAAATA 
GAAAGTAATA AAATGGAAAC ATTTTCTGAT GTTTCCATGA AAACAAATGT AGGAGAGTAC 
TACTCCAAAG AGCCAGAGAA CTATTTTGAA ACAGAAGCAG TGGAAATTGC CAAAGCTTTT 
ATGGAAGATG ATGAGCTGAC GGATTCTGAA CAGACTCATG CCAAATGCTC ACTGTTTGCA 
TGCCCCCAAA ACGAGGCTTT ATTAAATTCG AGAACTAGAA AAAGAGGAGG AATGGCTGGT 
GTTGCAGTTG GACAACCCCC AATTAAAAGG AGCTTATTAA ATGAATTTGA CAGGATAATA 
GAAAGTAAAG GAAAATCCTT AACGCCTTCA AAAAGCACTC CAGATGGCAC AATAAAAGAC 
AGACGATTGT TCACACACCA CATGTCTTTA GAGCCGGTTA CCTGTGGACC CTTCTGCTCA 
AGTAAAGAAA GGCAAGAAAC CCAGAGCCCA CATGTTACCT CACCTGCTCA AGGACTTCAG 
TCTAAGGGGC ATCCTTCAAG ACACTCAGCT GTGGGAAAGT CTTCAAGCAA TCCTACAGTT 
TCTGCCCTAA GATCTGAAAG GACCAGACAC TCAGTCTCAG ACAAATCCAC CAAAGTCTTT 
GTCCCACCTT TCAAGGTGAA ATCACGGTTT CACAGAGATG AACATTTTGA TAGCAAGAAT 
GTTAATTTGG AGGGAAAAAA CCAAAAGAGC GCAGATGGAG TCAGTGAAGA TGGGAACGAC 
AGTGACTTTC CTCAGTTTAA CAAAGATTTA ATGTCAAGCC TTCAGAATGC CAGAGACCTA 
CAGGATATAC GAATTAAAAA CAAAGAAAGG CATCATCTCT GTCCGCAGCC AGGCAGTCTG 
TATCTTACAA AATCATCCAC CCTGCCCCGG ATTTCTCTGC AGGCCGCAGT AGGAGACAGC 
GTTCCTTCTG CCTGTTCTCC TAAGCAGCTC TATATGTATG GCGTTTCTAA AGCATGCATA 
AGCGTTAACA GCAAAAACGC TGAGTATTTT CAGTTTGCCA TTGAGGATCA CTTTGGTAAA 
GAAGCTCTGT GCGCTGGCAA AGGCTTTCGG TTGGCAGATG GTGGATGGCT AATCCCCTCC 
GATGACGGGA AGGCTGGGAA AGAAGAATTT TACAGGGCTC TGTGTGACAC TCCAGGTGTG 
GATCCAAAGC TGATTTCTAG CGTCTGGGTC TCTAACCACT ACAGGTGGAT TGTATGGAAA 
CTGGCAGCTA TGGAATTTGC TTTTCCTAAG GAATTCGCGA ATAGATGTCT AAACCCAGAG 
CGAGTGCTGC TTCAACTAAA ATACAGATAC GATGTGGAAA TTGACAATAG CAGCAGATCA 
GCTCTCAAGA AGATACTGGA AAGGGATGAT ACAGCAGCAA AAACGCTTGT CCTCTGTGTT 
TCTGATATCA TTTCACTAAG CACAAATGTG TCTGAAACTT CAGGCAGTAA AGCTAGCAGT 
GAGGACAGTA ACAAAGTAGA CACGATCGAA CTCACAGATG GGTGGTATGC TGTCAAGGCC 
CAGCTAGACC CTCCACTCCT GGCTCTCGTA AAGAGTGGGA GACTCACTGT GGGTCAGAAG 
ATCATTACTC AGGGAGCAGA GCTGGTGGGC TCTCCCGATG CCTGTGCACC TCTGGAAGCC 
CCAGACTCCC TTAGGCTAAA GATTTCTGCA AACAGCACGC GGCCTGCTCG CTGGCACAGC 
AAGCTGGGGT TCTTTCATGA CCCCAGGCCC TTCCCTCTGC CCTTGTCCTC ACTGTTCAGT 
GATGGAGGAA ATGTTGGTTG TGTGGATGTC ATCGTTCAGA GAGTGTACCC TCTACAGTGG 
GTGGAGAAGA CGGTGTCTGG ATCGTACATA TTTCGTAATG AGAGAGAGGA GGAGAAGGAA 
GCGCTGAGAT TTGCAGAGGC CCAGCAGAAG AAACTAGAGG CCTTGTTCAC CAAAGTCCAC 
ACAGAGCTTA AAGAGCATGA AGAAGACATA GCCCAGCGGC GTGTGCTGTC CCGGGCACTC 
ACACGGCAGC AGGTCCACGC TCTGCAGGAC GGTGCAGAGC TCTATGCAGC AGTGCAGGAT 
GCATCAGACC CAGAGCACCT GGAGACTTGT TTCAGCGAAG AGCAGCTGAG AGCCTTGAAC 
AACTACAGAC AAATGCTGAG CGATAAGAAA CAAGCGCGGA TCCAGTCAGA GTTCCGGAAG 
GCCCTGGAGG CCGCTGAGAA AGAAGAGGGT TTATCAAGGG ACGTCTCAAC TGTGTGGAAG 
CTTCGTGTTA CAAGCTACAA GAAAAGAGAA AAATCAGCTC TGTTGAGTAT CTGGCGTCCA 
TCTTCAGACT TGCCCTCCCT GTTAACAGAA GGACAGAGAT ACAGAATCTA TCATCTTTCG 
GTGTCAAAAT CGAAGAATAA ATTTGAGTGG CCCAGCATCC AGTTAACAGC CACAAAGAGA 
ACTCAGTATC AACAGCTGCC GGTTTCCAGT GAGACCCTGC TCCAGCTTTA CCAGCCCAGG 
GAGCTCCTTC CCTTCAGCAA ACTGTCAGAT CCAGCCTTCC AGCCACCTTG TTCTGAAGTG 
GATGTAGTAG GAGTTGTAGT TTCTGTTGTA AAACCAATAG GTCTTGCTCC TTTGGTCTAC 
TTGTCAGACG AGTGCCTTCA TTTATTAGTG GTGAAATTTG GAATAGACCT TAATGAAGAC 
ATAAAGCCAC GTGTGCTTAT TGCTGCGAGC AACCTCCAGT GGCGGCCGGA GTCCACGTCA 
CGAGTGCCAA CTTTATTTGC TGGGAACTTT TCCGTATTCT CTGCCAGTCC AAAGGAGGCC 
CACTTTCAGG AGAGGGTCAC GAACATGAAG CATGCTATTG AGAACATCGA CACATTTTAC 
AAGGAGGCAG AAAAGAAGCT TATACAGGTG CTGAAGGGAG ACAGTCCAAA GTGGTCCACC 
CCGAACAAAG ACCCCACCCG AGAACCCTAC CCAGCCTCCA CTTGCTCTGC TTCAGACCTT 
GCTTCAGGAG AGGAGTTCAC CTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017453862.1
CDS	352..9798
Translation	MTVEYKRRPTFWEIFKARCSTADLGPISLNWFEELSSEAPPYNTEPPEESEYKPQGHEPQLFKTPQRNPSYHQFASTPIMFKEQSQTLPLDQSPFKELGNVVANSKRKHHSKKKARKDPVVDVASLPLKACPSESPCTPRCTQVAPQRRKPVVSGSLFYTPKLEETPKHISESLGVEVDPDMSWTSSLATPPTLSSTVLIARDEEAHRNAFPADSPASLKSYFSNHNESLKKNDRFIPSVSDSENKSQQEAFSQGLEKMLGDSSSKINRFRDCLRKPIPNVLEDGETAVDTSGEDSFSLCFPKRRTRNLQKTRMGKMKKKIFSETRTDGLSEEARGQADDKNSFALEIEPRDSEPLDPSVTNQKPLYSQSGDISSEAGQCSDSIWSQPDPSGLNGTQTRKIPLLHISSHKQSILEDFIDMKKEGTGSITFPHISSLPEPEKMFSEETLVDKEHEGQHLESLEDSISGKQMVSGTSQTACLSPSIRKSIVKMREPLEETLDTVFSDSMTSSAFTEELDASAGGLEIHTACSQREDSLCPSSVDTGSWPTTLTDTSATVKNAGLITTLKNKRRKFIYSVSDDASHQGKKLQTQRQSELTNLSAPFEASAFEVPFPFTNVDSGIPDSSIKRSNLPNDPEEPSLSLTNSFVTAASKEISYIHALISQDLNDKEAILSEEKPQPYTALEADFLSCLPERSCENDQKSPKVSDRKEKVLVSACRPSGRLAAAVQLSSISFDSQENPLGSHNVTSTLKLTPSPKTPLSKPVVVSRGKMCKMPEKLQCKSCKDNIELSKNIPLGVNEMCVLSENSETPELLPPLEYITEVSSSVKSQFNQNTKIAVVQKDQKDSTFISEVTVHMNSEELFPEKENNFAFQVTNESNKPNIGSTVEFQEEDLSHTKGHSLKNSPMTVDRDLDDEQAGQVLITEDSDSLAVVHDCTKKSRNTIEQHQKGTADKDFKSNSSLYLKSDGNNDYLDKWSEFLDPLMNHKFGGSFRTASNKEIKLSEDNVKKSKMFFKDIEEQYPTSLDCIDTVSTLQLANKKRLSEPHTFDLKSGTTVSTQCHSQSSVSHEDTHTAPQMLSSKQDFHSSHNLTPSQKAEITELSTILEESGSQFEFTQFKNPSHIAQNNTSAVLGNQMAVVRTASEEWKDVDLHLPLNPSSVGQIDHNKKFECLVGVKQSSSHLLEDTCNQNTSCFLPIKEMEFGGFCSALGTKLSVSNEALRKAMKLFSDIENISEEPSTKVGPRGFSSCAHHDSVASVFKIKKQNTDKSFDEKSSKCQVTVQNNKEMTTCILVDENPENYVKNIKQDNNYTGSQRNAYKLENSDVSKSSTSGTVYINKGDSDLPFAAEKGNKYPESCTQYVREENAQIKESVSDLTCLEVMKAEETCHMKSSDKEQLPSDKMEQNMKEFNISFQTASGKNIRVSKESLNKSVNILDQETEDLTVTSDSLNSKILCGINKDKMHISCHKKSINIKKVFEEHFPIGTVSQLPALQQYPEYEIESIKEPTLLSFHTASGKKVKIMQESLDKVKNLFDETQYVRKTTNFGHQESKPLKDREDYKERLTLAYEKIEVTASKCEEMQNFVSKQTEMLPQQNDHMYRQTENLTSNGSSPKVHGNIENKIEKNPRICCICQSSYFVTEDSALACYTGDSRKTCVGESSLSKGKKWLREQSDKLGTRNTIEIQCVKEHTEDFAGNALYEHSLVIIRTEIDTSHVSENQASTLFSDPNVCHSYLSHSSFCHHDDMHNDSGYFLKDKIDSDVQPDMKNTEGNAIFPKISATKEIKLHPQTVNEECVQKLETNASPYANKNIAIDSAMLDLRNCKVGSPVFITTHSQETVRMKEIFTDNCSKIVEQNRESKPDTCQTSCHKALDNSEDFICPSSSGDVCINSPMAIFYPQSEQILQHNQSVSGLKKAATPPVSLETWDTCKSIRGSPQEVHPSRTYGIFSTASGKAVQVSDASLEKARQVFSEIDGDAKQLASMVSLEGNEKSHHSVKRESSVVHNTHGVLSLRKTLPGNVSSSVFSGFSTAGGKLVTVSESALHKVKGMLEEFDLIRTEHTLQHSPTPEDVSKIPPQPCLESRTPEYSVSSKLQKTYNDKSRSPSNYKESGSSGNTQSLEVSPQLSQMERKQETQSVLGTKVSQRKTNILEKKQNLPQNIKIESNKMETFSDVSMKTNVGEYYSKEPENYFETEAVEIAKAFMEDDELTDSEQTHAKCSLFACPQNEALLNSRTRKRGGMAGVAVGQPPIKRSLLNEFDRIIESKGKSLTPSKSTPDGTIKDRRLFTHHMSLEPVTCGPFCSSKERQETQSPHVTSPAQGLQSKGHPSRHSAVGKSSSNPTVSALRSERTRHSVSDKSTKVFVPPFKVKSRFHRDEHFDSKNVNLEGKNQKSADGVSEDGNDSDFPQFNKDLMSSLQNARDLQDIRIKNKERHHLCPQPGSLYLTKSSTLPRISLQAAVGDSVPSACSPKQLYMYGVSKACISVNSKNAEYFQFAIEDHFGKEALCAGKGFRLADGGWLIPSDDGKAGKEEFYRALCDTPGVDPKLISSVWVSNHYRWIVWKLAAMEFAFPKEFANRCLNPERVLLQLKYRYDVEIDNSSRSALKKILERDDTAAKTLVLCVSDIISLSTNVSETSGSKASSEDSNKVDTIELTDGWYAVKAQLDPPLLALVKSGRLTVGQKIITQGAELVGSPDACAPLEAPDSLRLKISANSTRPARWHSKLGFFHDPRPFPLPLSSLFSDGGNVGCVDVIVQRVYPLQWVEKTVSGSYIFRNEREEEKEALRFAEAQQKKLEALFTKVHTELKEHEEDIAQRRVLSRALTRQQVHALQDGAELYAAVQDASDPEHLETCFSEEQLRALNNYRQMLSDKKQARIQSEFRKALEAAEKEEGLSRDVSTVWKLRVTSYKKREKSALLSIWRPSSDLPSLLTEGQRYRIYHLSVSKSKNKFEWPSIQLTATKRTQYQQLPVSSETLLQLYQPRELLPFSKLSDPAFQPPCSEVDVVGVVVSVVKPIGLAPLVYLSDECLHLLVVKFGIDLNEDIKPRVLIAASNLQWRPESTSRVPTLFAGNFSVFSASPKEAHFQERVTNMKHAIENIDTFYKEAEKKLIQVLKGDSPKWSTPNKDPTREPYPASTCSASDLASGEEFTY

Target ORF information:

RefSeq Version	XM_017598373.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus BRCA2, DNA repair associated (Brca2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017598373.1

ORF Insert Sequence:

ATGACCGTTG AATACAAAAG GAGACCAACT TTTTGGGAAA TTTTTAAAGC GAGATGCAGC 
ACAGCAGATT TAGGACCAAT AAGCCTCAAT TGGTTTGAAG AACTTTCTTC AGAAGCCCCA 
CCATATAATA CTGAACCTCC AGAAGAATCT GAATATAAAC CCCAAGGCCA TGAACCACAG 
CTATTTAAAA CACCACAGAG GAATCCCTCT TACCATCAGT TTGCTTCAAC TCCAATAATG 
TTCAAAGAAC AAAGTCAAAC TCTACCACTA GACCAGTCAC CTTTCAAAGA ATTAGGGAAT 
GTTGTTGCAA ATAGTAAACG TAAACATCAC AGCAAAAAGA AGGCCAGAAA GGACCCTGTG 
GTAGATGTTG CCAGTCTGCC GCTGAAAGCT TGTCCCAGTG AAAGCCCTTG TACTCCGCGA 
TGCACACAGG TGGCACCGCA GCGAAGAAAG CCAGTGGTAT CTGGAAGTTT ATTCTATACA 
CCAAAACTTG AGGAGACACC AAAACATATT TCTGAAAGTC TGGGAGTTGA AGTGGATCCG 
GATATGTCTT GGACAAGTTC ATTAGCTACA CCACCAACCC TTAGTTCCAC TGTGCTCATA 
GCCCGAGATG AAGAAGCACA CAGAAATGCA TTTCCAGCCG ACTCTCCTGC TAGTTTGAAA 
AGCTATTTTT CTAACCACAA TGAAAGTCTG AAAAAGAATG ATAGATTTAT TCCCTCTGTG 
TCTGACAGTG AAAACAAAAG CCAGCAAGAA GCTTTTAGTC AGGGACTGGA GAAAATGTTA 
GGGGATTCAT CTAGCAAAAT AAATCGCTTC AGAGACTGCC TTAGAAAACC AATACCAAAT 
GTTCTAGAAG ATGGGGAGAC AGCTGTAGAT ACTTCAGGAG AAGATAGTTT CTCGTTATGT 
TTTCCTAAAC GTAGAACCAG AAATCTACAG AAAACGAGAA TGGGCAAGAT GAAGAAGAAA 
ATCTTCAGTG AAACAAGGAC TGATGGATTA AGTGAAGAAG CCAGAGGACA AGCTGATGAT 
AAAAACTCAT TTGCACTTGA AATTGAACCA AGAGATAGTG AGCCCTTAGA TCCATCTGTA 
ACAAACCAGA AGCCCCTTTA CAGCCAGAGT GGGGACATCT CCAGTGAAGC TGGCCAGTGT 
TCAGACAGTA TATGGTCTCA GCCAGATCCC TCTGGTCTAA ATGGAACCCA GACAAGAAAA 
ATACCCCTAC TCCATATTTC TTCTCATAAG CAAAGTATTT TAGAAGACTT CATAGATATG 
AAGAAGGAAG GTACTGGCTC TATTACTTTT CCTCATATTT CTAGTCTTCC AGAACCAGAA 
AAGATGTTCA GTGAGGAAAC TCTGGTAGAC AAGGAACATG AAGGACAGCA TCTTGAGTCA 
CTTGAAGACT CCATCTCAGG GAAACAAATG GTGTCTGGAA CGAGTCAAAC AGCCTGTCTC 
TCTCCGAGTA TCAGGAAGTC TATAGTCAAG ATGAGAGAGC CACTTGAAGA GACTTTGGAT 
ACTGTTTTCT CAGACAGTAT GACCAGCTCA GCCTTTACAG AAGAACTTGA CGCCTCTGCA 
GGGGGACTGG AAATACATAC TGCATGCTCA CAGAGAGAGG ATTCTTTATG TCCTAGTTCA 
GTGGACACTG GAAGCTGGCC AACCACTCTC ACTGACACTT CTGCAACTGT GAAAAATGCA 
GGTTTAATAA CCACTCTCAA AAATAAAAGA AGAAAGTTTA TTTACTCTGT AAGTGATGAT 
GCATCTCATC AAGGAAAAAA ACTACAGACA CAGAGACAGT CAGAGCTTAC TAACCTTTCA 
GCCCCGTTTG AAGCAAGTGC TTTTGAAGTA CCATTCCCCT TTACAAATGT AGATTCAGGT 
ATACCAGATT CTTCAATCAA AAGAAGCAAT TTACCAAATG ATCCTGAAGA GCCATCTTTG 
TCCTTGACCA ACTCTTTTGT GACTGCTGCC AGTAAAGAAA TTAGTTATAT TCATGCATTG 
ATATCTCAGG ATCTAAATGA CAAAGAAGCA ATACTCAGTG AAGAAAAGCC ACAGCCATAT 
ACAGCCCTAG AAGCTGACTT TCTGTCGTGC TTGCCAGAAA GATCATGTGA AAATGATCAA 
AAAAGTCCAA AAGTTTCCGA CCGAAAAGAA AAAGTCTTAG TCTCAGCATG TCGTCCTTCA 
GGAAGGCTTG CAGCAGCAGT GCAGCTCAGC AGCATTAGCT TTGACTCTCA GGAAAACCCT 
CTTGGTAGCC ACAACGTAAC AAGTACTCTT AAATTAACTC CCAGCCCGAA GACACCTCTG 
TCAAAGCCAG TTGTGGTTTC TAGAGGGAAA ATGTGTAAAA TGCCAGAGAA ACTGCAATGT 
AAGAGTTGTA AAGATAATAT TGAATTAAGC AAAAACATCC CTCTGGGGGT TAATGAAATG 
TGTGTCTTAA GTGAAAATTC TGAAACACCT GAGCTTCTGC CACCTCTAGA ATATATAACA 
GAAGTGTCCT CATCAGTGAA GTCACAGTTC AATCAAAATA CAAAAATAGC AGTCGTACAA 
AAGGACCAAA AAGACTCAAC TTTTATTTCA GAAGTAACAG TCCATATGAA TTCTGAAGAA 
CTTTTCCCAG AAAAGGAGAA TAATTTTGCT TTTCAAGTAA CCAATGAAAG CAATAAACCC 
AATATAGGAA GTACTGTGGA ATTCCAGGAA GAAGACCTCA GCCACACAAA AGGGCATAGT 
CTCAAGAACT CTCCCATGAC AGTAGATAGA GACCTAGATG ATGAGCAAGC AGGCCAAGTG 
TTGATTACAG AGGACTCAGA TTCATTAGCA GTAGTCCATG ATTGTACAAA GAAGAGCAGA 
AATACTATAG AGCAGCATCA GAAAGGAACT GCAGACAAAG ACTTCAAGTC AAATTCCTCC 
TTGTATTTGA AATCAGATGG GAACAATGAT TATTTAGACA AATGGTCAGA GTTCTTGGAT 
CCACTCATGA ACCATAAATT TGGAGGTAGC TTCAGAACAG CTTCCAATAA AGAAATAAAA 
CTTTCAGAGG ATAATGTCAA GAAAAGCAAA ATGTTCTTCA AAGATATCGA AGAACAGTAT 
CCTACTAGTT TAGATTGTAT TGACACTGTT AGTACCCTAC AATTAGCAAA CAAGAAGAGA 
CTAAGTGAAC CTCATACATT TGATTTGAAG TCAGGTACTA CTGTATCTAC ACAGTGTCAT 
AGTCAATCAT CTGTTTCTCA TGAAGATACT CACACAGCAC CTCAGATGTT ATCTTCAAAG 
CAAGATTTTC ATTCAAGTCA TAACTTAACG CCCAGCCAAA AAGCAGAAAT TACAGAACTG 
TCTACTATCT TGGAAGAATC AGGAAGTCAG TTTGAATTCA CACAGTTCAA AAATCCAAGC 
CACATAGCAC AGAATAATAC ATCTGCAGTG CTTGGAAACC AGATGGCTGT TGTAAGGACC 
GCTTCTGAGG AGTGGAAAGA TGTTGATCTT CATCTCCCAC TGAATCCCTC CTCTGTAGGT 
CAGATAGATC ACAACAAGAA ATTCGAATGT TTGGTTGGAG TTAAGCAAAG CTCTTCTCAC 
CTGTTAGAAG ACACTTGTAA CCAAAATACA TCTTGTTTTT TACCGATAAA AGAAATGGAG 
TTTGGAGGAT TTTGTTCTGC TCTTGGCACA AAACTTAGTG TGTCTAATGA GGCTCTGAGA 
AAGGCTATGA AACTGTTCAG TGACATTGAA AATATTAGTG AGGAGCCTTC TACAAAAGTA 
GGACCAAGAG GATTCTCTTC ATGTGCACAT CATGATTCTG TTGCTTCCGT GTTTAAGATA 
AAGAAACAAA ACACTGATAA AAGTTTTGAT GAGAAATCTA GTAAGTGCCA GGTAACAGTA 
CAAAATAATA AGGAAATGAC TACCTGTATT CTTGTTGATG AAAATCCTGA AAATTATGTA 
AAGAATATAA AACAAGATAA CAACTATACT GGCTCTCAAA GAAATGCTTA TAAATTAGAA 
AACTCTGATG TTAGTAAATC AAGTACAAGT GGCACAGTTT ATATTAATAA AGGTGACAGT 
GATTTACCTT TTGCTGCTGA AAAAGGCAAT AAGTATCCTG AGTCATGTAC CCAATATGTG 
AGGGAAGAAA ATGCACAAAT TAAGGAAAGT GTATCAGATT TAACATGTTT GGAAGTCATG 
AAAGCTGAGG AAACATGTCA TATGAAATCT TCAGATAAAG AACAATTACC TTCAGATAAG 
ATGGAACAAA ATATGAAAGA GTTTAATATA TCTTTTCAGA CTGCAAGCGG GAAAAATATC 
AGAGTCTCCA AAGAGTCACT AAATAAAAGT GTGAATATTT TAGATCAGGA AACAGAAGAC 
TTGACTGTCA CTTCAGATTC TTTGAATTCT AAAATTCTTT GTGGCATAAA TAAGGACAAA 
ATGCATATTT CATGTCACAA GAAATCAATC AATATTAAAA AGGTATTTGA AGAACATTTC 
CCAATTGGAA CTGTCAGTCA ATTACCAGCT CTTCAGCAGT ATCCTGAATA TGAAATAGAA 
AGTATCAAAG AACCTACTCT GTTGAGTTTT CATACAGCTA GTGGGAAAAA AGTCAAAATT 
ATGCAGGAAT CTTTGGACAA AGTGAAAAAT CTTTTTGATG AGACACAGTA TGTTAGGAAA 
ACCACCAATT TTGGTCATCA AGAATCAAAA CCCCTGAAGG ACAGAGAGGA CTATAAAGAA 
AGACTTACAT TAGCATATGA GAAAATTGAA GTAACTGCCT CAAAATGTGA AGAAATGCAG 
AACTTTGTCT CTAAGCAGAC TGAAATGCTA CCCCAGCAAA ATGATCATAT GTATAGGCAA 
ACTGAAAATC TCACATCAAA TGGTAGCTCT CCCAAAGTAC ATGGAAACAT AGAAAATAAA 
ATAGAAAAGA ATCCTAGAAT TTGCTGTATT TGTCAGTCCT CATACTTTGT CACTGAAGAT 
TCTGCTTTGG CATGTTATAC GGGGGACAGT AGAAAAACTT GTGTCGGAGA GTCTTCTCTG 
TCCAAAGGCA AAAAATGGCT TAGAGAACAA AGTGATAAGC TTGGAACAAG AAATACTATT 
GAAATCCAAT GTGTAAAGGA ACACACTGAA GATTTTGCAG GAAATGCCTT ATATGAACAT 
AGTTTAGTCA TTATCAGAAC TGAAATTGAT ACAAGTCATG TCTCTGAAAA CCAAGCTTCA 
ACCCTCTTTA GTGACCCTAA TGTGTGTCAC AGCTATCTAT CCCATTCTAG TTTTTGTCAT 
CATGATGATA TGCATAATGA TTCAGGATAT TTCTTAAAAG ATAAAATTGA TTCTGATGTC 
CAGCCAGACA TGAAGAATAC TGAAGGCAAT GCCATTTTCC CTAAAATATC TGCTACAAAA 
GAAATAAAAC TACACCCACA AACTGTAAAT GAAGAGTGTG TTCAAAAACT GGAGACTAAT 
GCTTCACCAT ATGCAAATAA AAATATAGCC ATTGACTCAG CTATGCTGGA TTTAAGGAAT 
TGTAAGGTAG GCTCACCTGT ATTCATTACA ACTCATTCAC AAGAAACTGT AAGAATGAAA 
GAGATATTCA CAGATAACTG TAGTAAAATA GTCGAACAAA ACAGGGAGAG TAAACCAGAC 
ACTTGCCAGA CAAGCTGTCA TAAAGCATTG GATAATTCAG AGGATTTTAT ATGTCCTAGC 
TCTTCAGGTG ATGTCTGCAT AAACTCACCT ATGGCTATTT TTTATCCTCA AAGTGAACAA 
ATTTTACAGC ATAACCAAAG TGTGTCTGGA CTGAAGAAAG CTGCAACACC ACCTGTTAGT 
TTGGAAACTT GGGATACATG TAAATCTATA AGAGGATCTC CTCAGGAAGT CCATCCTTCA 
CGCACTTATG GAATTTTTAG CACAGCAAGT GGAAAAGCTG TACAAGTATC TGATGCTTCA 
TTGGAAAAGG CACGGCAAGT GTTTTCTGAG ATAGATGGTG ATGCTAAACA GTTAGCTTCC 
ATGGTGTCAC TGGAAGGTAA TGAAAAATCA CATCACTCTG TGAAAAGAGA AAGCTCTGTG 
GTGCATAACA CCCATGGTGT ATTGTCACTC CGAAAAACCC TCCCAGGCAA TGTCAGTTCA 
TCTGTATTCT CTGGATTTAG CACCGCAGGT GGAAAACTGG TCACAGTTTC AGAAAGTGCT 
TTACATAAAG TTAAAGGAAT GTTAGAGGAA TTTGATTTGA TCAGAACTGA ACATACTCTC 
CAGCATTCAC CTACACCTGA AGACGTATCA AAAATACCTC CTCAACCTTG TCTTGAAAGC 
AGAACCCCAG AATACTCTGT AAGCTCTAAA TTGCAGAAAA CCTACAATGA TAAATCCAGG 
TCACCAAGTA ATTATAAAGA AAGTGGTTCT TCAGGCAATA CTCAATCTCT TGAAGTTTCT 
CCCCAACTCT CTCAGATGGA GAGAAAGCAA GAAACACAGT CGGTATTAGG AACAAAAGTG 
TCCCAGAGGA AGACTAATAT CTTGGAAAAA AAACAAAACT TACCCCAAAA CATAAAAATA 
GAAAGTAATA AAATGGAAAC ATTTTCTGAT GTTTCCATGA AAACAAATGT AGGAGAGTAC 
TACTCCAAAG AGCCAGAGAA CTATTTTGAA ACAGAAGCAG TGGAAATTGC CAAAGCTTTT 
ATGGAAGATG ATGAGCTGAC GGATTCTGAA CAGACTCATG CCAAATGCTC ACTGTTTGCA 
TGCCCCCAAA ACGAGGCTTT ATTAAATTCG AGAACTAGAA AAAGAGGAGG AATGGCTGGT 
GTTGCAGTTG GACAACCCCC AATTAAAAGG AGCTTATTAA ATGAATTTGA CAGGATAATA 
GAAAGTAAAG GAAAATCCTT AACGCCTTCA AAAAGCACTC CAGATGGCAC AATAAAAGAC 
AGACGATTGT TCACACACCA CATGTCTTTA GAGCCGGTTA CCTGTGGACC CTTCTGCTCA 
AGTAAAGAAA GGCAAGAAAC CCAGAGCCCA CATGTTACCT CACCTGCTCA AGGACTTCAG 
TCTAAGGGGC ATCCTTCAAG ACACTCAGCT GTGGGAAAGT CTTCAAGCAA TCCTACAGTT 
TCTGCCCTAA GATCTGAAAG GACCAGACAC TCAGTCTCAG ACAAATCCAC CAAAGTCTTT 
GTCCCACCTT TCAAGGTGAA ATCACGGTTT CACAGAGATG AACATTTTGA TAGCAAGAAT 
GTTAATTTGG AGGGAAAAAA CCAAAAGAGC GCAGATGGAG TCAGTGAAGA TGGGAACGAC 
AGTGACTTTC CTCAGTTTAA CAAAGATTTA ATGTCAAGCC TTCAGAATGC CAGAGACCTA 
CAGGATATAC GAATTAAAAA CAAAGAAAGG CATCATCTCT GTCCGCAGCC AGGCAGTCTG 
TATCTTACAA AATCATCCAC CCTGCCCCGG ATTTCTCTGC AGGCCGCAGT AGGAGACAGC 
GTTCCTTCTG CCTGTTCTCC TAAGCAGCTC TATATGTATG GCGTTTCTAA AGCATGCATA 
AGCGTTAACA GCAAAAACGC TGAGTATTTT CAGTTTGCCA TTGAGGATCA CTTTGGTAAA 
GAAGCTCTGT GCGCTGGCAA AGGCTTTCGG TTGGCAGATG GTGGATGGCT AATCCCCTCC 
GATGACGGGA AGGCTGGGAA AGAAGAATTT TACAGGGCTC TGTGTGACAC TCCAGGTGTG 
GATCCAAAGC TGATTTCTAG CGTCTGGGTC TCTAACCACT ACAGGTGGAT TGTATGGAAA 
CTGGCAGCTA TGGAATTTGC TTTTCCTAAG GAATTCGCGA ATAGATGTCT AAACCCAGAG 
CGAGTGCTGC TTCAACTAAA ATACAGATAC GATGTGGAAA TTGACAATAG CAGCAGATCA 
GCTCTCAAGA AGATACTGGA AAGGGATGAT ACAGCAGCAA AAACGCTTGT CCTCTGTGTT 
TCTGATATCA TTTCACTAAG CACAAATGTG TCTGAAACTT CAGGCAGTAA AGCTAGCAGT 
GAGGACAGTA ACAAAGTAGA CACGATCGAA CTCACAGATG GGTGGTATGC TGTCAAGGCC 
CAGCTAGACC CTCCACTCCT GGCTCTCGTA AAGAGTGGGA GACTCACTGT GGGTCAGAAG 
ATCATTACTC AGGGAGCAGA GCTGGTGGGC TCTCCCGATG CCTGTGCACC TCTGGAAGCC 
CCAGACTCCC TTAGGCTAAA GATTTCTGCA AACAGCACGC GGCCTGCTCG CTGGCACAGC 
AAGCTGGGGT TCTTTCATGA CCCCAGGCCC TTCCCTCTGC CCTTGTCCTC ACTGTTCAGT 
GATGGAGGAA ATGTTGGTTG TGTGGATGTC ATCGTTCAGA GAGTGTACCC TCTACAGTGG 
GTGGAGAAGA CGGTGTCTGG ATCGTACATA TTTCGTAATG AGAGAGAGGA GGAGAAGGAA 
GCGCTGAGAT TTGCAGAGGC CCAGCAGAAG AAACTAGAGG CCTTGTTCAC CAAAGTCCAC 
ACAGAGCTTA AAGAGCATGA AGAAGACATA GCCCAGCGGC GTGTGCTGTC CCGGGCACTC 
ACACGGCAGC AGGTCCACGC TCTGCAGGAC GGTGCAGAGC TCTATGCAGC AGTGCAGGAT 
GCATCAGACC CAGAGCACCT GGAGACTTGT TTCAGCGAAG AGCAGCTGAG AGCCTTGAAC 
AACTACAGAC AAATGCTGAG CGATAAGAAA CAAGCGCGGA TCCAGTCAGA GTTCCGGAAG 
GCCCTGGAGG CCGCTGAGAA AGAAGAGGGT TTATCAAGGG ACGTCTCAAC TGTGTGGAAG 
CTTCGTGTTA CAAGCTACAA GAAAAGAGAA AAATCAGCTC TGTTGAGTAT CTGGCGTCCA 
TCTTCAGACT TGCCCTCCCT GTTAACAGAA GGACAGAGAT ACAGAATCTA TCATCTTTCG 
GTGTCAAAAT CGAAGAATAA ATTTGAGTGG CCCAGCATCC AGTTAACAGC CACAAAGAGA 
ACTCAGTATC AACAGCTGCC GGTTTCCAGT GAGACCCTGC TCCAGCTTTA CCAGCCCAGG 
GAGCTCCTTC CCTTCAGCAA ACTGTCAGAT CCAGCCTTCC AGCCACCTTG TTCTGAAGTG 
GATGTAGTAG GAGTTGTAGT TTCTGTTGTA AAACCAATAG GTCTTGCTCC TTTGGTCTAC 
TTGTCAGACG AGTGCCTTCA TTTATTAGTG GTGAAATTTG GAATAGACCT TAATGAAGAC 
ATAAAGCCAC GTGTGCTTAT TGCTGCGAGC AACCTCCAGT GGCGGCCGGA GTCCACGTCA 
CGAGTGCCAA CTTTATTTGC TGGGAACTTT TCCGTATTCT CTGCCAGTCC AAAGGAGGCC 
CACTTTCAGG AGAGGGTCAC GAACATGAAG CATGCTATTG AGAACATCGA CACATTTTAC 
AAGGAGGCAG AAAAGAAGCT TATACAGGTG CTGAAGGGAG ACAGTCCAAA GTGGTCCACC 
CCGAACAAAG ACCCCACCCG AGAACCCTAC CCAGCCTCCA CTTGCTCTGC TTCAGACCTT 
GCTTCAGGAG AGGAGTTCAC CTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Brca2 ( XM_017598374.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017453863.1 Rattus norvegicus BRCA2, DNA repair associated (Brca2), transcript variant X3, mRNA.

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CloneID	ORa57846
Clone ID Related Accession (Same CDS sequence)	XM_017598374.1
Accession Version	XM_017598374.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9363bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	breast cancer type 2 susceptibility protein homolog isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005111.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCGTTG AATACAAAAG GAGACCAACT TTTTGGGAAA TTTTTAAAGC GAGATGCAGC 
ACAGCAGATT TAGGACCAAT AAGCCTCAAT TGGTTTGAAG AACTTTCTTC AGAAGCCCCA 
CCATATAATA CTGAACCTCC AGAAGAATCT GAATATAAAC CCCAAGGCCA TGAACCACAG 
CTATTTAAAA CACCACAGAG GAATCCCTCT TACCATCAGT TTGCTTCAAC TCCAATAATG 
TTCAAAGAAC AAAGTCAAAC TCTACCACTA GACCAGTCAC CTTTCAAAGA ATTAGGGAAT 
GTTGTTGCAA ATAGTAAACG TAAACATCAC AGCAAAAAGA AGGCCAGAAA GGACCCTGTG 
GTAGATGTTG CCAGTCTGCC GCTGAAAGCT TGTCCCAGTG AAAGCCCTTG TACTCCGCGA 
TGCACACAGG TGGCACCGCA GCGAAGAAAG CCAGTGGTAT CTGGAAGTTT ATTCTATACA 
CCAAAACTTG AGGAGACACC AAAACATATT TCTGAAAGTC TGGGAGTTGA AGTGGATCCG 
GATATGTCTT GGACAAGTTC ATTAGCTACA CCACCAACCC TTAGTTCCAC TGTGCTCATA 
GCCCGAGATG AAGAAGCACA CAGAAATGCA TTTCCAGCCG ACTCTCCTGC TAGTTTGAAA 
AGCTATTTTT CTAACCACAA TGAAAGTCTG AAAAAGAATG ATAGATTTAT TCCCTCTGTG 
TCTGACAGTG AAAACAAAAG CCAGCAAGAA GCTTTTAGTC AGGGACTGGA GAAAATGTTA 
GGGGATTCAT CTAGCAAAAT AAATCGCTTC AGAGACTGCC TTAGAAAACC AATACCAAAT 
GTTCTAGAAG ATGGGGAGAC AGCTGTAGAT ACTTCAGGAG AAGATAGTTT CTCGTTATGT 
TTTCCTAAAC GTAGAACCAG AAATCTACAG AAAACGAGAA TGGGCAAGAT GAAGAAGAAA 
ATCTTCAGTG AAACAAGGAC TGATGGATTA AGTGAAGAAG CCAGAGGACA AGCTGATGAT 
AAAAACTCAT TTGCACTTGA AATTGAACCA AGAGATAGTG AGCCCTTAGA TCCATCTGTA 
ACAAACCAGA AGCCCCTTTA CAGCCAGAGT GGGGACATCT CCAGTGAAGC TGGCCAGTGT 
TCAGACAGTA TATGGTCTCA GCCAGATCCC TCTGGTCTAA ATGGAACCCA GACAAGAAAA 
ATACCCCTAC TCCATATTTC TTCTCATAAG CAAAGTATTT TAGAAGACTT CATAGATATG 
AAGAAGGAAG GTACTGGCTC TATTACTTTT CCTCATATTT CTAGTCTTCC AGAACCAGAA 
AAGATGTTCA GTGAGGAAAC TCTGGTAGAC AAGGAACATG AAGGACAGCA TCTTGAGTCA 
CTTGAAGACT CCATCTCAGG GAAACAAATG GTGTCTGGAA CGAGTCAAAC AGCCTGTCTC 
TCTCCGAGTA TCAGGAAGTC TATAGTCAAG ATGAGAGAGC CACTTGAAGA GACTTTGGAT 
ACTGTTTTCT CAGACAGTAT GACCAGCTCA GCCTTTACAG AAGAACTTGA CGCCTCTGCA 
GGGGGACTGG AAATACATAC TGCATGCTCA CAGAGAGAGG ATTCTTTATG TCCTAGTTCA 
GTGGACACTG GAAGCTGGCC AACCACTCTC ACTGACACTT CTGCAACTGT GAAAAATGCA 
GGTTTAATAA CCACTCTCAA AAATAAAAGA AGAAAGTTTA TTTACTCTGT AAGTGATGAT 
GCATCTCATC AAGGAAAAAA ACTACAGACA CAGAGACAGT CAGAGCTTAC TAACCTTTCA 
GCCCCGTTTG AAGCAAGTGC TTTTGAAGTA CCATTCCCCT TTACAAATGT AGATTCAGGT 
ATACCAGATT CTTCAATCAA AAGAAGCAAT TTACCAAATG ATCCTGAAGA GCCATCTTTG 
TCCTTGACCA ACTCTTTTGT GACTGCTGCC AGTAAAGAAA TTAGTTATAT TCATGCATTG 
ATATCTCAGG ATCTAAATGA CAAAGAAGCA ATACTCAGTG AAGAAAAGCC ACAGCCATAT 
ACAGCCCTAG AAGCTGACTT TCTGTCGTGC TTGCCAGAAA GATCATGTGA AAATGATCAA 
AAAAGTCCAA AAGTTTCCGA CCGAAAAGAA AAAGTCTTAG TCTCAGCATG TCGTCCTTCA 
GGAAGGCTTG CAGCAGCAGT GCAGCTCAGC AGCATTAGCT TTGACTCTCA GGAAAACCCT 
CTTGGTAGCC ACAACGTAAC AAGTACTCTT AAATTAACTC CCAGCCCGAA GACACCTCTG 
TCAAAGCCAG TTGTGGTTTC TAGAGGGAAA ATGTGTAAAA TGCCAGAGAA ACTGCAATGT 
AAGAGTTGTA AAGATAATAT TGAATTAAGC AAAAACATCC CTCTGGGGGT TAATGAAATG 
TGTGTCTTAA GTGAAAATTC TGAAACACCT GAGCTTCTGC CACCTCTAGA ATATATAACA 
GAAGTGTCCT CATCAGTGAA GTCACAGTTC AATCAAAATA CAAAAATAGC AGTCGTACAA 
AAGGACCAAA AAGACTCAAC TTTTATTTCA GAAGTAACAG TCCATATGAA TTCTGAAGAA 
CTTTTCCCAG AAAAGGAGAA TAATTTTGCT TTTCAAGTAA CCAATGAAAG CAATAAACCC 
AATATAGGAA GTACTGTGGA ATTCCAGGAA GAAGACCTCA GCCACACAAA AGGGCATAGT 
CTCAAGAACT CTCCCATGAC AGTAGATAGA GACCTAGATG ATGAGCAAGC AGGCCAAGTG 
TTGATTACAG AGGACTCAGA TTCATTAGCA GTAGTCCATG ATTGTACAAA GAAGAGCAGA 
AATACTATAG AGCAGCATCA GAAAGGAACT GCAGACAAAG ACTTCAAGTC AAATTCCTCC 
TTGTATTTGA AATCAGATGG GAACAATGAT TATTTAGACA AATGGTCAGA GTTCTTGGAT 
CCACTCATGA ACCATAAATT TGGAGGTAGC TTCAGAACAG CTTCCAATAA AGAAATAAAA 
CTTTCAGAGG ATAATGTCAA GAAAAGCAAA ATGTTCTTCA AAGATATCGA AGAACAGTAT 
CCTACTAGTT TAGATTGTAT TGACACTGTT AGTACCCTAC AATTAGCAAA CAAGAAGAGA 
CTAAGTGAAC CTCATACATT TGATTTGAAG TCAGGTACTA CTGTATCTAC ACAGTGTCAT 
AGTCAATCAT CTGTTTCTCA TGAAGATACT CACACAGCAC CTCAGATGTT ATCTTCAAAG 
CAAGATTTTC ATTCAAGTCA TAACTTAACG CCCAGCCAAA AAGCAGAAAT TACAGAACTG 
TCTACTATCT TGGAAGAATC AGGAAGTCAG TTTGAATTCA CACAGTTCAA AAATCCAAGC 
CACATAGCAC AGAATAATAC ATCTGCAGTG CTTGGAAACC AGATGGCTGT TGTAAGGACC 
GCTTCTGAGG AGTGGAAAGA TGTTGATCTT CATCTCCCAC TGAATCCCTC CTCTGTAGGT 
CAGATAGATC ACAACAAGAA ATTCGAATGT TTGGTTGGAG TTAAGCAAAG CTCTTCTCAC 
CTGTTAGAAG ACACTTGTAA CCAAAATACA TCTTGTTTTT TACCGATAAA AGAAATGGAG 
TTTGGAGGAT TTTGTTCTGC TCTTGGCACA AAACTTAGTG TGTCTAATGA GGCTCTGAGA 
AAGGCTATGA AACTGTTCAG TGACATTGAA AATATTAGTG AGGAGCCTTC TACAAAAGTA 
GGACCAAGAG GATTCTCTTC ATGTGCACAT CATGATTCTG TTGCTTCCGT GTTTAAGATA 
AAGAAACAAA ACACTGATAA AAGTTTTGAT GAGAAATCTA GTAAGTGCCA GGTAACAGTA 
CAAAATAATA AGGAAATGAC TACCTGTATT CTTGTTGATG AAAATCCTGA AAATTATGTA 
AAGAATATAA AACAAGATAA CAACTATACT GGCTCTCAAA GAAATGCTTA TAAATTAGAA 
AACTCTGATG TTAGTAAATC AAGTACAAGT GGCACAGTTT ATATTAATAA AGGTGACAGT 
GATTTACCTT TTGCTGCTGA AAAAGGCAAT AAGTATCCTG AGTCATGTAC CCAATATGTG 
AGGGAAGAAA ATGCACAAAT TAAGGAAAGT GTATCAGATT TAACATGTTT GGAAGTCATG 
AAAGCTGAGG AAACATGTCA TATGAAATCT TCAGATAAAG AACAATTACC TTCAGATAAG 
ATGGAACAAA ATATGAAAGA GTTTAATATA TCTTTTCAGA CTGCAAGCGG GAAAAATATC 
AGAGTCTCCA AAGAGTCACT AAATAAAAGT GTGAATATTT TAGATCAGGA AACAGAAGAC 
TTGACTGTCA CTTCAGATTC TTTGAATTCT AAAATTCTTT GTGGCATAAA TAAGGACAAA 
ATGCATATTT CATGTCACAA GAAATCAATC AATATTAAAA AGGTATTTGA AGAACATTTC 
CCAATTGGAA CTGTCAGTCA ATTACCAGCT CTTCAGCAGT ATCCTGAATA TGAAATAGAA 
AGTATCAAAG AACCTACTCT GTTGAGTTTT CATACAGCTA GTGGGAAAAA AGTCAAAATT 
ATGCAGGAAT CTTTGGACAA AGTGAAAAAT CTTTTTGATG AGACACAGTA TGTTAGGAAA 
ACCACCAATT TTGGTCATCA AGAATCAAAA CCCCTGAAGG ACAGAGAGGA CTATAAAGAA 
AGACTTACAT TAGCATATGA GAAAATTGAA GTAACTGCCT CAAAATGTGA AGAAATGCAG 
AACTTTGTCT CTAAGCAGAC TGAAATGCTA CCCCAGCAAA ATGATCATAT GTATAGGCAA 
ACTGAAAATC TCACATCAAA TGGTAGCTCT CCCAAAGTAC ATGGAAACAT AGAAAATAAA 
ATAGAAAAGA ATCCTAGAAT TTGCTGTATT TGTCAGTCCT CATACTTTGT CACTGAAGAT 
TCTGCTTTGG CATGTTATAC GGGGGACAGT AGAAAAACTT GTGTCGGAGA GTCTTCTCTG 
TCCAAAGGCA AAAAATGGCT TAGAGAACAA AGTGATAAGC TTGGAACAAG AAATACTATT 
GAAATCCAAT GTGTAAAGGA ACACACTGAA GATTTTGCAG GAAATGCCTT ATATGAACAT 
AGTTTAGTCA TTATCAGAAC TGAAATTGAT ACAAGTCATG TCTCTGAAAA CCAAGCTTCA 
ACCCTCTTTA GTGACCCTAA TGTGTGTCAC AGCTATCTAT CCCATTCTAG TTTTTGTCAT 
CATGATGATA TGCATAATGA TTCAGGATAT TTCTTAAAAG ATAAAATTGA TTCTGATGTC 
CAGCCAGACA TGAAGAATAC TGAAGGCAAT GCCATTTTCC CTAAAATATC TGCTACAAAA 
GAAATAAAAC TACACCCACA AACTGTAAAT GAAGAGTGTG TTCAAAAACT GGAGACTAAT 
GCTTCACCAT ATGCAAATAA AAATATAGCC ATTGACTCAG CTATGCTGGA TTTAAGGAAT 
TGTAAGGTAG GCTCACCTGT ATTCATTACA ACTCATTCAC AAGAAACTGT AAGAATGAAA 
GAGATATTCA CAGATAACTG TAGTAAAATA GTCGAACAAA ACAGGGAGAG TAAACCAGAC 
ACTTGCCAGA CAAGCTGTCA TAAAGCATTG GATAATTCAG AGGATTTTAT ATGTCCTAGC 
TCTTCAGGTG ATGTCTGCAT AAACTCACCT ATGGCTATTT TTTATCCTCA AAGTGAACAA 
ATTTTACAGC ATAACCAAAG TGTGTCTGGA CTGAAGAAAG CTGCAACACC ACCTGTTAGT 
TTGGAAACTT GGGATACATG TAAATCTATA AGAGGATCTC CTCAGGAAGT CCATCCTTCA 
CGCACTTATG GAATTTTTAG CACAGCAAGT GGAAAAGCTG TACAAGTATC TGATGCTTCA 
TTGGAAAAGG CACGGCAAGT GTTTTCTGAG ATAGATGGTG ATGCTAAACA GTTAGCTTCC 
ATGGTGTCAC TGGAAGGTAA TGAAAAATCA CATCACTCTG TGAAAAGAGA AAGCTCTGTG 
GTGCATAACA CCCATGGTGT ATTGTCACTC CGAAAAACCC TCCCAGGCAA TGTCAGTTCA 
TCTGTATTCT CTGGATTTAG CACCGCAGGT GGAAAACTGG TCACAGTTTC AGAAAGTGCT 
TTACATAAAG TTAAAGGAAT GTTAGAGGAA TTTGATTTGA TCAGAACTGA ACATACTCTC 
CAGCATTCAC CTACACCTGA AGACGTATCA AAAATACCTC CTCAACCTTG TCTTGAAAGC 
AGAACCCCAG AATACTCTGT AAGCTCTAAA TTGCAGAAAA CCTACAATGA TAAATCCAGG 
TCACCAAGTA ATTATAAAGA AAGTGGTTCT TCAGGCAATA CTCAATCTCT TGAAGTTTCT 
CCCCAACTCT CTCAGATGGA GAGAAAGCAA GAAACACAGT CGGTATTAGG AACAAAAGTG 
TCCCAGAGGA AGACTAATAT CTTGGAAAAA AAACAAAACT TACCCCAAAA CATAAAAATA 
GAAAGTAATA AAATGGAAAC ATTTTCTGAT GTTTCCATGA AAACAAATGT AGGAGAGTAC 
TACTCCAAAG AGCCAGAGAA CTATTTTGAA ACAGAAGCAG TGGAAATTGC CAAAGCTTTT 
ATGGAAGATG ATGAGCTGAC GGATTCTGAA CAGACTCATG CCAAATGCTC ACTGTTTGCA 
TGCCCCCAAA ACGAGGCTTT ATTAAATTCG AGAACTAGAA AAAGAGGAGG AATGGCTGGT 
GTTGCAGTTG GACAACCCCC AATTAAAAGG AGCTTATTAA ATGAATTTGA CAGGATAATA 
GAAAGTAAAG GAAAATCCTT AACGCCTTCA AAAAGCACTC CAGATGGCAC AATAAAAGAC 
AGACGATTGT TCACACACCA CATGTCTTTA GAGCCGGTTA CCTGTGGACC CTTCTGCTCA 
AGTAAAGAAA GGCAAGAAAC CCAGAGCCCA CATGTTACCT CACCTGCTCA AGGACTTCAG 
TCTAAGGGGC ATCCTTCAAG ACACTCAGCT GTGGGAAAGT CTTCAAGCAA TCCTACAGTT 
TCTGCCCTAA GATCTGAAAG GACCAGACAC TCAGTCTCAG ACAAATCCAC CAAAGTCTTT 
GTCCCACCTT TCAAGGTGAA ATCACGGTTT CACAGAGATG AACATTTTGA TAGCAAGAAT 
GTTAATTTGG AGGGAAAAAA CCAAAAGAGC GCAGATGGAG TCAGTGAAGA TGGGAACGAC 
AGTGACTTTC CTCAGTTTAA CAAAGATTTA ATGTCAAGCC TTCAGAATGC CAGAGACCTA 
CAGGATATAC GAATTAAAAA CAAAGAAAGG CATCATCTCT GTCCGCAGCC AGGCAGTCTG 
TATCTTACAA AATCATCCAC CCTGCCCCGG ATTTCTCTGC AGGCCGCAGT AGGAGACAGC 
GTTCCTTCTG CCTGTTCTCC TAAGCAGCTC TATATGTATG GCGTTTCTAA AGCATGCATA 
AGCGTTAACA GCAAAAACGC TGAGTATTTT CAGTTTGCCA TTGAGGATCA CTTTGGTAAA 
GAAGCTCTGT GCGCTGGCAA AGGCTTTCGG TTGGCAGATG GTGGATGGCT AATCCCCTCC 
GATGACGGGA AGGCTGGGAA AGAAGAATTT TACAGGGCTC TGTGTGACAC TCCAGGTGTG 
GATCCAAAGC TGATTTCTAG CGTCTGGGTC TCTAACCACT ACAGGTGGAT TGTATGGAAA 
CTGGCAGCTA TGGAATTTGC TTTTCCTAAG GAATTCGCGA ATAGATGTCT AAACCCAGAG 
CGAGTGCTGC TTCAACTAAA ATACAGATAC GATGTGGAAA TTGACAATAG CAGCAGATCA 
GCTCTCAAGA AGATACTGGA AAGGGATGAT ACAGCAGCAA AAACGCTTGT CCTCTGTGTT 
TCTGATATCA TTTCACTAAG CACAAATGTG TCTGAAACTT CAGGCAGTAA AGCTAGCAGT 
GAGGACAGTA ACAAAGTAGA CACGATCGAA CTCACAGATG GGTGGTATGC TGTCAAGGCC 
CAGCTAGACC CTCCACTCCT GGCTCTCGTA AAGAGTGGGA GACTCACTGT GGGTCAGAAG 
ATCATTACTC AGGGAGCAGA GCTGGTGGGC TCTCCCGATG CCTGTGCACC TCTGGAAGCC 
CCAGACTCCC TTAGGCTAAA GATTTCTGCA AACAGCACGC GGCCTGCTCG CTGGCACAGC 
AAGCTGGGGT TCTTTCATGA CCCCAGGCCC TTCCCTCTGC CCTTGTCCTC ACTGTTCAGT 
GATGGAGGAA ATGTTGGTTG TGTGGATGTC ATCGTTCAGA GAGTGTACCC TCTACAGTGG 
GTGGAGAAGA CGGTGTCTGG ATCGTACATA TTTCGTAATG AGAGAGAGGA GGAGAAGGAA 
GCGCTGAGAT TTGCAGAGGC CCAGCAGAAG AAACTAGAGG CCTTGTTCAC CAAAGTCCAC 
ACAGAGCTTA AAGAGCATGA AGAAGACATA GCCCAGCGGC GTGTGCTGTC CCGGGCACTC 
ACACGGCAGC AGGTCCACGC TCTGCAGGAC GGTGCAGAGC TCTATGCAGC AGTGCAGGAT 
GCATCAGACC CAGAGCACCT GGAGACTTGT TTCAGCGAAG AGCAGCTGAG AGCCTTGAAC 
AACTACAGAC AAATGCTGAG CGATAAGAAA CAAGCGCGGA TCCAGTCAGA GTTCCGGAAG 
GCCCTGGAGG CCGCTGAGAA AGAAGAGGGT TTATCAAGGG ACGTCTCAAC TGTGTGGAAG 
CTTCGTGTTA CAAGCTACAA GAAAAGAGAA AAATCAGCTC TGTTGAGTAT CTGGCGTCCA 
TCTTCAGACT TGCCCTCCCT GTTAACAGAA GGACAGAGAT ACAGAATCTA TCATCTTTCG 
GTGTCAAAAT CGAAGAATAA ATTTGAGTGG CCCAGCATCC AGTTAACAGC CACAAAGAGA 
ACTCAGTATC AACAGCTGCC GGTTTCCAGT GAGACCCTGC TCCAGCTTTA CCAGCCCAGG 
GAGCTCCTTC CCTTCAGCAA ACTGTCAGAT CCAGCCTTCC AGCCACCTTG TTCTGAAGTG 
GATGTAGTAG GAGTTGTAGT TTCTGTTGTA AAACCAATAG AACATCGACA CATTTTACAA 
GGAGGCAGAA AAGAAGCTTA TACAGGTGCT GAAGGGAGAC AGTCCAAAGT GGTCCACCCC 
GAACAAAGAC CCCACCCGAG AACCCTACCC AGCCTCCACT TGCTCTGCTT CAGACCTTGC 
TTCAGGAGAG GAGTTCACCT ACTGATCAGC AAAGTTATCG AAGTCCTTTA TCATGCTGCA 
CACCGACGGG GAAATCTACA CCCCTGGCCC ACTCAGCCTG GATGGCAGCC AAGTCTTGTA 
GTGGGGAGAA TGAGATTGAG GACCCCAAGA CCTGTCGAAA AAAAAGGGCC TTGGATCTCC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017453863.1
CDS	352..9714
Translation	MTVEYKRRPTFWEIFKARCSTADLGPISLNWFEELSSEAPPYNTEPPEESEYKPQGHEPQLFKTPQRNPSYHQFASTPIMFKEQSQTLPLDQSPFKELGNVVANSKRKHHSKKKARKDPVVDVASLPLKACPSESPCTPRCTQVAPQRRKPVVSGSLFYTPKLEETPKHISESLGVEVDPDMSWTSSLATPPTLSSTVLIARDEEAHRNAFPADSPASLKSYFSNHNESLKKNDRFIPSVSDSENKSQQEAFSQGLEKMLGDSSSKINRFRDCLRKPIPNVLEDGETAVDTSGEDSFSLCFPKRRTRNLQKTRMGKMKKKIFSETRTDGLSEEARGQADDKNSFALEIEPRDSEPLDPSVTNQKPLYSQSGDISSEAGQCSDSIWSQPDPSGLNGTQTRKIPLLHISSHKQSILEDFIDMKKEGTGSITFPHISSLPEPEKMFSEETLVDKEHEGQHLESLEDSISGKQMVSGTSQTACLSPSIRKSIVKMREPLEETLDTVFSDSMTSSAFTEELDASAGGLEIHTACSQREDSLCPSSVDTGSWPTTLTDTSATVKNAGLITTLKNKRRKFIYSVSDDASHQGKKLQTQRQSELTNLSAPFEASAFEVPFPFTNVDSGIPDSSIKRSNLPNDPEEPSLSLTNSFVTAASKEISYIHALISQDLNDKEAILSEEKPQPYTALEADFLSCLPERSCENDQKSPKVSDRKEKVLVSACRPSGRLAAAVQLSSISFDSQENPLGSHNVTSTLKLTPSPKTPLSKPVVVSRGKMCKMPEKLQCKSCKDNIELSKNIPLGVNEMCVLSENSETPELLPPLEYITEVSSSVKSQFNQNTKIAVVQKDQKDSTFISEVTVHMNSEELFPEKENNFAFQVTNESNKPNIGSTVEFQEEDLSHTKGHSLKNSPMTVDRDLDDEQAGQVLITEDSDSLAVVHDCTKKSRNTIEQHQKGTADKDFKSNSSLYLKSDGNNDYLDKWSEFLDPLMNHKFGGSFRTASNKEIKLSEDNVKKSKMFFKDIEEQYPTSLDCIDTVSTLQLANKKRLSEPHTFDLKSGTTVSTQCHSQSSVSHEDTHTAPQMLSSKQDFHSSHNLTPSQKAEITELSTILEESGSQFEFTQFKNPSHIAQNNTSAVLGNQMAVVRTASEEWKDVDLHLPLNPSSVGQIDHNKKFECLVGVKQSSSHLLEDTCNQNTSCFLPIKEMEFGGFCSALGTKLSVSNEALRKAMKLFSDIENISEEPSTKVGPRGFSSCAHHDSVASVFKIKKQNTDKSFDEKSSKCQVTVQNNKEMTTCILVDENPENYVKNIKQDNNYTGSQRNAYKLENSDVSKSSTSGTVYINKGDSDLPFAAEKGNKYPESCTQYVREENAQIKESVSDLTCLEVMKAEETCHMKSSDKEQLPSDKMEQNMKEFNISFQTASGKNIRVSKESLNKSVNILDQETEDLTVTSDSLNSKILCGINKDKMHISCHKKSINIKKVFEEHFPIGTVSQLPALQQYPEYEIESIKEPTLLSFHTASGKKVKIMQESLDKVKNLFDETQYVRKTTNFGHQESKPLKDREDYKERLTLAYEKIEVTASKCEEMQNFVSKQTEMLPQQNDHMYRQTENLTSNGSSPKVHGNIENKIEKNPRICCICQSSYFVTEDSALACYTGDSRKTCVGESSLSKGKKWLREQSDKLGTRNTIEIQCVKEHTEDFAGNALYEHSLVIIRTEIDTSHVSENQASTLFSDPNVCHSYLSHSSFCHHDDMHNDSGYFLKDKIDSDVQPDMKNTEGNAIFPKISATKEIKLHPQTVNEECVQKLETNASPYANKNIAIDSAMLDLRNCKVGSPVFITTHSQETVRMKEIFTDNCSKIVEQNRESKPDTCQTSCHKALDNSEDFICPSSSGDVCINSPMAIFYPQSEQILQHNQSVSGLKKAATPPVSLETWDTCKSIRGSPQEVHPSRTYGIFSTASGKAVQVSDASLEKARQVFSEIDGDAKQLASMVSLEGNEKSHHSVKRESSVVHNTHGVLSLRKTLPGNVSSSVFSGFSTAGGKLVTVSESALHKVKGMLEEFDLIRTEHTLQHSPTPEDVSKIPPQPCLESRTPEYSVSSKLQKTYNDKSRSPSNYKESGSSGNTQSLEVSPQLSQMERKQETQSVLGTKVSQRKTNILEKKQNLPQNIKIESNKMETFSDVSMKTNVGEYYSKEPENYFETEAVEIAKAFMEDDELTDSEQTHAKCSLFACPQNEALLNSRTRKRGGMAGVAVGQPPIKRSLLNEFDRIIESKGKSLTPSKSTPDGTIKDRRLFTHHMSLEPVTCGPFCSSKERQETQSPHVTSPAQGLQSKGHPSRHSAVGKSSSNPTVSALRSERTRHSVSDKSTKVFVPPFKVKSRFHRDEHFDSKNVNLEGKNQKSADGVSEDGNDSDFPQFNKDLMSSLQNARDLQDIRIKNKERHHLCPQPGSLYLTKSSTLPRISLQAAVGDSVPSACSPKQLYMYGVSKACISVNSKNAEYFQFAIEDHFGKEALCAGKGFRLADGGWLIPSDDGKAGKEEFYRALCDTPGVDPKLISSVWVSNHYRWIVWKLAAMEFAFPKEFANRCLNPERVLLQLKYRYDVEIDNSSRSALKKILERDDTAAKTLVLCVSDIISLSTNVSETSGSKASSEDSNKVDTIELTDGWYAVKAQLDPPLLALVKSGRLTVGQKIITQGAELVGSPDACAPLEAPDSLRLKISANSTRPARWHSKLGFFHDPRPFPLPLSSLFSDGGNVGCVDVIVQRVYPLQWVEKTVSGSYIFRNEREEEKEALRFAEAQQKKLEALFTKVHTELKEHEEDIAQRRVLSRALTRQQVHALQDGAELYAAVQDASDPEHLETCFSEEQLRALNNYRQMLSDKKQARIQSEFRKALEAAEKEEGLSRDVSTVWKLRVTSYKKREKSALLSIWRPSSDLPSLLTEGQRYRIYHLSVSKSKNKFEWPSIQLTATKRTQYQQLPVSSETLLQLYQPRELLPFSKLSDPAFQPPCSEVDVVGVVVSVVKPIEHRHILQGGRKEAYTGAEGRQSKVVHPEQRPHPRTLPSLHLLCFRPCFRRGVHLLISKVIEVLYHAAHRRGNLHPWPTQPGWQPSLVVGRMRLRTPRPVEKKGPWIS

Target ORF information:

RefSeq Version	XM_017598374.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus BRCA2, DNA repair associated (Brca2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017598374.1

ORF Insert Sequence:

ATGACCGTTG AATACAAAAG GAGACCAACT TTTTGGGAAA TTTTTAAAGC GAGATGCAGC 
ACAGCAGATT TAGGACCAAT AAGCCTCAAT TGGTTTGAAG AACTTTCTTC AGAAGCCCCA 
CCATATAATA CTGAACCTCC AGAAGAATCT GAATATAAAC CCCAAGGCCA TGAACCACAG 
CTATTTAAAA CACCACAGAG GAATCCCTCT TACCATCAGT TTGCTTCAAC TCCAATAATG 
TTCAAAGAAC AAAGTCAAAC TCTACCACTA GACCAGTCAC CTTTCAAAGA ATTAGGGAAT 
GTTGTTGCAA ATAGTAAACG TAAACATCAC AGCAAAAAGA AGGCCAGAAA GGACCCTGTG 
GTAGATGTTG CCAGTCTGCC GCTGAAAGCT TGTCCCAGTG AAAGCCCTTG TACTCCGCGA 
TGCACACAGG TGGCACCGCA GCGAAGAAAG CCAGTGGTAT CTGGAAGTTT ATTCTATACA 
CCAAAACTTG AGGAGACACC AAAACATATT TCTGAAAGTC TGGGAGTTGA AGTGGATCCG 
GATATGTCTT GGACAAGTTC ATTAGCTACA CCACCAACCC TTAGTTCCAC TGTGCTCATA 
GCCCGAGATG AAGAAGCACA CAGAAATGCA TTTCCAGCCG ACTCTCCTGC TAGTTTGAAA 
AGCTATTTTT CTAACCACAA TGAAAGTCTG AAAAAGAATG ATAGATTTAT TCCCTCTGTG 
TCTGACAGTG AAAACAAAAG CCAGCAAGAA GCTTTTAGTC AGGGACTGGA GAAAATGTTA 
GGGGATTCAT CTAGCAAAAT AAATCGCTTC AGAGACTGCC TTAGAAAACC AATACCAAAT 
GTTCTAGAAG ATGGGGAGAC AGCTGTAGAT ACTTCAGGAG AAGATAGTTT CTCGTTATGT 
TTTCCTAAAC GTAGAACCAG AAATCTACAG AAAACGAGAA TGGGCAAGAT GAAGAAGAAA 
ATCTTCAGTG AAACAAGGAC TGATGGATTA AGTGAAGAAG CCAGAGGACA AGCTGATGAT 
AAAAACTCAT TTGCACTTGA AATTGAACCA AGAGATAGTG AGCCCTTAGA TCCATCTGTA 
ACAAACCAGA AGCCCCTTTA CAGCCAGAGT GGGGACATCT CCAGTGAAGC TGGCCAGTGT 
TCAGACAGTA TATGGTCTCA GCCAGATCCC TCTGGTCTAA ATGGAACCCA GACAAGAAAA 
ATACCCCTAC TCCATATTTC TTCTCATAAG CAAAGTATTT TAGAAGACTT CATAGATATG 
AAGAAGGAAG GTACTGGCTC TATTACTTTT CCTCATATTT CTAGTCTTCC AGAACCAGAA 
AAGATGTTCA GTGAGGAAAC TCTGGTAGAC AAGGAACATG AAGGACAGCA TCTTGAGTCA 
CTTGAAGACT CCATCTCAGG GAAACAAATG GTGTCTGGAA CGAGTCAAAC AGCCTGTCTC 
TCTCCGAGTA TCAGGAAGTC TATAGTCAAG ATGAGAGAGC CACTTGAAGA GACTTTGGAT 
ACTGTTTTCT CAGACAGTAT GACCAGCTCA GCCTTTACAG AAGAACTTGA CGCCTCTGCA 
GGGGGACTGG AAATACATAC TGCATGCTCA CAGAGAGAGG ATTCTTTATG TCCTAGTTCA 
GTGGACACTG GAAGCTGGCC AACCACTCTC ACTGACACTT CTGCAACTGT GAAAAATGCA 
GGTTTAATAA CCACTCTCAA AAATAAAAGA AGAAAGTTTA TTTACTCTGT AAGTGATGAT 
GCATCTCATC AAGGAAAAAA ACTACAGACA CAGAGACAGT CAGAGCTTAC TAACCTTTCA 
GCCCCGTTTG AAGCAAGTGC TTTTGAAGTA CCATTCCCCT TTACAAATGT AGATTCAGGT 
ATACCAGATT CTTCAATCAA AAGAAGCAAT TTACCAAATG ATCCTGAAGA GCCATCTTTG 
TCCTTGACCA ACTCTTTTGT GACTGCTGCC AGTAAAGAAA TTAGTTATAT TCATGCATTG 
ATATCTCAGG ATCTAAATGA CAAAGAAGCA ATACTCAGTG AAGAAAAGCC ACAGCCATAT 
ACAGCCCTAG AAGCTGACTT TCTGTCGTGC TTGCCAGAAA GATCATGTGA AAATGATCAA 
AAAAGTCCAA AAGTTTCCGA CCGAAAAGAA AAAGTCTTAG TCTCAGCATG TCGTCCTTCA 
GGAAGGCTTG CAGCAGCAGT GCAGCTCAGC AGCATTAGCT TTGACTCTCA GGAAAACCCT 
CTTGGTAGCC ACAACGTAAC AAGTACTCTT AAATTAACTC CCAGCCCGAA GACACCTCTG 
TCAAAGCCAG TTGTGGTTTC TAGAGGGAAA ATGTGTAAAA TGCCAGAGAA ACTGCAATGT 
AAGAGTTGTA AAGATAATAT TGAATTAAGC AAAAACATCC CTCTGGGGGT TAATGAAATG 
TGTGTCTTAA GTGAAAATTC TGAAACACCT GAGCTTCTGC CACCTCTAGA ATATATAACA 
GAAGTGTCCT CATCAGTGAA GTCACAGTTC AATCAAAATA CAAAAATAGC AGTCGTACAA 
AAGGACCAAA AAGACTCAAC TTTTATTTCA GAAGTAACAG TCCATATGAA TTCTGAAGAA 
CTTTTCCCAG AAAAGGAGAA TAATTTTGCT TTTCAAGTAA CCAATGAAAG CAATAAACCC 
AATATAGGAA GTACTGTGGA ATTCCAGGAA GAAGACCTCA GCCACACAAA AGGGCATAGT 
CTCAAGAACT CTCCCATGAC AGTAGATAGA GACCTAGATG ATGAGCAAGC AGGCCAAGTG 
TTGATTACAG AGGACTCAGA TTCATTAGCA GTAGTCCATG ATTGTACAAA GAAGAGCAGA 
AATACTATAG AGCAGCATCA GAAAGGAACT GCAGACAAAG ACTTCAAGTC AAATTCCTCC 
TTGTATTTGA AATCAGATGG GAACAATGAT TATTTAGACA AATGGTCAGA GTTCTTGGAT 
CCACTCATGA ACCATAAATT TGGAGGTAGC TTCAGAACAG CTTCCAATAA AGAAATAAAA 
CTTTCAGAGG ATAATGTCAA GAAAAGCAAA ATGTTCTTCA AAGATATCGA AGAACAGTAT 
CCTACTAGTT TAGATTGTAT TGACACTGTT AGTACCCTAC AATTAGCAAA CAAGAAGAGA 
CTAAGTGAAC CTCATACATT TGATTTGAAG TCAGGTACTA CTGTATCTAC ACAGTGTCAT 
AGTCAATCAT CTGTTTCTCA TGAAGATACT CACACAGCAC CTCAGATGTT ATCTTCAAAG 
CAAGATTTTC ATTCAAGTCA TAACTTAACG CCCAGCCAAA AAGCAGAAAT TACAGAACTG 
TCTACTATCT TGGAAGAATC AGGAAGTCAG TTTGAATTCA CACAGTTCAA AAATCCAAGC 
CACATAGCAC AGAATAATAC ATCTGCAGTG CTTGGAAACC AGATGGCTGT TGTAAGGACC 
GCTTCTGAGG AGTGGAAAGA TGTTGATCTT CATCTCCCAC TGAATCCCTC CTCTGTAGGT 
CAGATAGATC ACAACAAGAA ATTCGAATGT TTGGTTGGAG TTAAGCAAAG CTCTTCTCAC 
CTGTTAGAAG ACACTTGTAA CCAAAATACA TCTTGTTTTT TACCGATAAA AGAAATGGAG 
TTTGGAGGAT TTTGTTCTGC TCTTGGCACA AAACTTAGTG TGTCTAATGA GGCTCTGAGA 
AAGGCTATGA AACTGTTCAG TGACATTGAA AATATTAGTG AGGAGCCTTC TACAAAAGTA 
GGACCAAGAG GATTCTCTTC ATGTGCACAT CATGATTCTG TTGCTTCCGT GTTTAAGATA 
AAGAAACAAA ACACTGATAA AAGTTTTGAT GAGAAATCTA GTAAGTGCCA GGTAACAGTA 
CAAAATAATA AGGAAATGAC TACCTGTATT CTTGTTGATG AAAATCCTGA AAATTATGTA 
AAGAATATAA AACAAGATAA CAACTATACT GGCTCTCAAA GAAATGCTTA TAAATTAGAA 
AACTCTGATG TTAGTAAATC AAGTACAAGT GGCACAGTTT ATATTAATAA AGGTGACAGT 
GATTTACCTT TTGCTGCTGA AAAAGGCAAT AAGTATCCTG AGTCATGTAC CCAATATGTG 
AGGGAAGAAA ATGCACAAAT TAAGGAAAGT GTATCAGATT TAACATGTTT GGAAGTCATG 
AAAGCTGAGG AAACATGTCA TATGAAATCT TCAGATAAAG AACAATTACC TTCAGATAAG 
ATGGAACAAA ATATGAAAGA GTTTAATATA TCTTTTCAGA CTGCAAGCGG GAAAAATATC 
AGAGTCTCCA AAGAGTCACT AAATAAAAGT GTGAATATTT TAGATCAGGA AACAGAAGAC 
TTGACTGTCA CTTCAGATTC TTTGAATTCT AAAATTCTTT GTGGCATAAA TAAGGACAAA 
ATGCATATTT CATGTCACAA GAAATCAATC AATATTAAAA AGGTATTTGA AGAACATTTC 
CCAATTGGAA CTGTCAGTCA ATTACCAGCT CTTCAGCAGT ATCCTGAATA TGAAATAGAA 
AGTATCAAAG AACCTACTCT GTTGAGTTTT CATACAGCTA GTGGGAAAAA AGTCAAAATT 
ATGCAGGAAT CTTTGGACAA AGTGAAAAAT CTTTTTGATG AGACACAGTA TGTTAGGAAA 
ACCACCAATT TTGGTCATCA AGAATCAAAA CCCCTGAAGG ACAGAGAGGA CTATAAAGAA 
AGACTTACAT TAGCATATGA GAAAATTGAA GTAACTGCCT CAAAATGTGA AGAAATGCAG 
AACTTTGTCT CTAAGCAGAC TGAAATGCTA CCCCAGCAAA ATGATCATAT GTATAGGCAA 
ACTGAAAATC TCACATCAAA TGGTAGCTCT CCCAAAGTAC ATGGAAACAT AGAAAATAAA 
ATAGAAAAGA ATCCTAGAAT TTGCTGTATT TGTCAGTCCT CATACTTTGT CACTGAAGAT 
TCTGCTTTGG CATGTTATAC GGGGGACAGT AGAAAAACTT GTGTCGGAGA GTCTTCTCTG 
TCCAAAGGCA AAAAATGGCT TAGAGAACAA AGTGATAAGC TTGGAACAAG AAATACTATT 
GAAATCCAAT GTGTAAAGGA ACACACTGAA GATTTTGCAG GAAATGCCTT ATATGAACAT 
AGTTTAGTCA TTATCAGAAC TGAAATTGAT ACAAGTCATG TCTCTGAAAA CCAAGCTTCA 
ACCCTCTTTA GTGACCCTAA TGTGTGTCAC AGCTATCTAT CCCATTCTAG TTTTTGTCAT 
CATGATGATA TGCATAATGA TTCAGGATAT TTCTTAAAAG ATAAAATTGA TTCTGATGTC 
CAGCCAGACA TGAAGAATAC TGAAGGCAAT GCCATTTTCC CTAAAATATC TGCTACAAAA 
GAAATAAAAC TACACCCACA AACTGTAAAT GAAGAGTGTG TTCAAAAACT GGAGACTAAT 
GCTTCACCAT ATGCAAATAA AAATATAGCC ATTGACTCAG CTATGCTGGA TTTAAGGAAT 
TGTAAGGTAG GCTCACCTGT ATTCATTACA ACTCATTCAC AAGAAACTGT AAGAATGAAA 
GAGATATTCA CAGATAACTG TAGTAAAATA GTCGAACAAA ACAGGGAGAG TAAACCAGAC 
ACTTGCCAGA CAAGCTGTCA TAAAGCATTG GATAATTCAG AGGATTTTAT ATGTCCTAGC 
TCTTCAGGTG ATGTCTGCAT AAACTCACCT ATGGCTATTT TTTATCCTCA AAGTGAACAA 
ATTTTACAGC ATAACCAAAG TGTGTCTGGA CTGAAGAAAG CTGCAACACC ACCTGTTAGT 
TTGGAAACTT GGGATACATG TAAATCTATA AGAGGATCTC CTCAGGAAGT CCATCCTTCA 
CGCACTTATG GAATTTTTAG CACAGCAAGT GGAAAAGCTG TACAAGTATC TGATGCTTCA 
TTGGAAAAGG CACGGCAAGT GTTTTCTGAG ATAGATGGTG ATGCTAAACA GTTAGCTTCC 
ATGGTGTCAC TGGAAGGTAA TGAAAAATCA CATCACTCTG TGAAAAGAGA AAGCTCTGTG 
GTGCATAACA CCCATGGTGT ATTGTCACTC CGAAAAACCC TCCCAGGCAA TGTCAGTTCA 
TCTGTATTCT CTGGATTTAG CACCGCAGGT GGAAAACTGG TCACAGTTTC AGAAAGTGCT 
TTACATAAAG TTAAAGGAAT GTTAGAGGAA TTTGATTTGA TCAGAACTGA ACATACTCTC 
CAGCATTCAC CTACACCTGA AGACGTATCA AAAATACCTC CTCAACCTTG TCTTGAAAGC 
AGAACCCCAG AATACTCTGT AAGCTCTAAA TTGCAGAAAA CCTACAATGA TAAATCCAGG 
TCACCAAGTA ATTATAAAGA AAGTGGTTCT TCAGGCAATA CTCAATCTCT TGAAGTTTCT 
CCCCAACTCT CTCAGATGGA GAGAAAGCAA GAAACACAGT CGGTATTAGG AACAAAAGTG 
TCCCAGAGGA AGACTAATAT CTTGGAAAAA AAACAAAACT TACCCCAAAA CATAAAAATA 
GAAAGTAATA AAATGGAAAC ATTTTCTGAT GTTTCCATGA AAACAAATGT AGGAGAGTAC 
TACTCCAAAG AGCCAGAGAA CTATTTTGAA ACAGAAGCAG TGGAAATTGC CAAAGCTTTT 
ATGGAAGATG ATGAGCTGAC GGATTCTGAA CAGACTCATG CCAAATGCTC ACTGTTTGCA 
TGCCCCCAAA ACGAGGCTTT ATTAAATTCG AGAACTAGAA AAAGAGGAGG AATGGCTGGT 
GTTGCAGTTG GACAACCCCC AATTAAAAGG AGCTTATTAA ATGAATTTGA CAGGATAATA 
GAAAGTAAAG GAAAATCCTT AACGCCTTCA AAAAGCACTC CAGATGGCAC AATAAAAGAC 
AGACGATTGT TCACACACCA CATGTCTTTA GAGCCGGTTA CCTGTGGACC CTTCTGCTCA 
AGTAAAGAAA GGCAAGAAAC CCAGAGCCCA CATGTTACCT CACCTGCTCA AGGACTTCAG 
TCTAAGGGGC ATCCTTCAAG ACACTCAGCT GTGGGAAAGT CTTCAAGCAA TCCTACAGTT 
TCTGCCCTAA GATCTGAAAG GACCAGACAC TCAGTCTCAG ACAAATCCAC CAAAGTCTTT 
GTCCCACCTT TCAAGGTGAA ATCACGGTTT CACAGAGATG AACATTTTGA TAGCAAGAAT 
GTTAATTTGG AGGGAAAAAA CCAAAAGAGC GCAGATGGAG TCAGTGAAGA TGGGAACGAC 
AGTGACTTTC CTCAGTTTAA CAAAGATTTA ATGTCAAGCC TTCAGAATGC CAGAGACCTA 
CAGGATATAC GAATTAAAAA CAAAGAAAGG CATCATCTCT GTCCGCAGCC AGGCAGTCTG 
TATCTTACAA AATCATCCAC CCTGCCCCGG ATTTCTCTGC AGGCCGCAGT AGGAGACAGC 
GTTCCTTCTG CCTGTTCTCC TAAGCAGCTC TATATGTATG GCGTTTCTAA AGCATGCATA 
AGCGTTAACA GCAAAAACGC TGAGTATTTT CAGTTTGCCA TTGAGGATCA CTTTGGTAAA 
GAAGCTCTGT GCGCTGGCAA AGGCTTTCGG TTGGCAGATG GTGGATGGCT AATCCCCTCC 
GATGACGGGA AGGCTGGGAA AGAAGAATTT TACAGGGCTC TGTGTGACAC TCCAGGTGTG 
GATCCAAAGC TGATTTCTAG CGTCTGGGTC TCTAACCACT ACAGGTGGAT TGTATGGAAA 
CTGGCAGCTA TGGAATTTGC TTTTCCTAAG GAATTCGCGA ATAGATGTCT AAACCCAGAG 
CGAGTGCTGC TTCAACTAAA ATACAGATAC GATGTGGAAA TTGACAATAG CAGCAGATCA 
GCTCTCAAGA AGATACTGGA AAGGGATGAT ACAGCAGCAA AAACGCTTGT CCTCTGTGTT 
TCTGATATCA TTTCACTAAG CACAAATGTG TCTGAAACTT CAGGCAGTAA AGCTAGCAGT 
GAGGACAGTA ACAAAGTAGA CACGATCGAA CTCACAGATG GGTGGTATGC TGTCAAGGCC 
CAGCTAGACC CTCCACTCCT GGCTCTCGTA AAGAGTGGGA GACTCACTGT GGGTCAGAAG 
ATCATTACTC AGGGAGCAGA GCTGGTGGGC TCTCCCGATG CCTGTGCACC TCTGGAAGCC 
CCAGACTCCC TTAGGCTAAA GATTTCTGCA AACAGCACGC GGCCTGCTCG CTGGCACAGC 
AAGCTGGGGT TCTTTCATGA CCCCAGGCCC TTCCCTCTGC CCTTGTCCTC ACTGTTCAGT 
GATGGAGGAA ATGTTGGTTG TGTGGATGTC ATCGTTCAGA GAGTGTACCC TCTACAGTGG 
GTGGAGAAGA CGGTGTCTGG ATCGTACATA TTTCGTAATG AGAGAGAGGA GGAGAAGGAA 
GCGCTGAGAT TTGCAGAGGC CCAGCAGAAG AAACTAGAGG CCTTGTTCAC CAAAGTCCAC 
ACAGAGCTTA AAGAGCATGA AGAAGACATA GCCCAGCGGC GTGTGCTGTC CCGGGCACTC 
ACACGGCAGC AGGTCCACGC TCTGCAGGAC GGTGCAGAGC TCTATGCAGC AGTGCAGGAT 
GCATCAGACC CAGAGCACCT GGAGACTTGT TTCAGCGAAG AGCAGCTGAG AGCCTTGAAC 
AACTACAGAC AAATGCTGAG CGATAAGAAA CAAGCGCGGA TCCAGTCAGA GTTCCGGAAG 
GCCCTGGAGG CCGCTGAGAA AGAAGAGGGT TTATCAAGGG ACGTCTCAAC TGTGTGGAAG 
CTTCGTGTTA CAAGCTACAA GAAAAGAGAA AAATCAGCTC TGTTGAGTAT CTGGCGTCCA 
TCTTCAGACT TGCCCTCCCT GTTAACAGAA GGACAGAGAT ACAGAATCTA TCATCTTTCG 
GTGTCAAAAT CGAAGAATAA ATTTGAGTGG CCCAGCATCC AGTTAACAGC CACAAAGAGA 
ACTCAGTATC AACAGCTGCC GGTTTCCAGT GAGACCCTGC TCCAGCTTTA CCAGCCCAGG 
GAGCTCCTTC CCTTCAGCAA ACTGTCAGAT CCAGCCTTCC AGCCACCTTG TTCTGAAGTG 
GATGTAGTAG GAGTTGTAGT TTCTGTTGTA AAACCAATAG AACATCGACA CATTTTACAA 
GGAGGCAGAA AAGAAGCTTA TACAGGTGCT GAAGGGAGAC AGTCCAAAGT GGTCCACCCC 
GAACAAAGAC CCCACCCGAG AACCCTACCC AGCCTCCACT TGCTCTGCTT CAGACCTTGC 
TTCAGGAGAG GAGTTCACCT ACTGATCAGC AAAGTTATCG AAGTCCTTTA TCATGCTGCA 
CACCGACGGG GAAATCTACA CCCCTGGCCC ACTCAGCCTG GATGGCAGCC AAGTCTTGTA 
GTGGGGAGAA TGAGATTGAG GACCCCAAGA CCTGTCGAAA AAAAAGGGCC TTGGATCTCC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Brca2 ( XM_017598375.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017453864.1 Rattus norvegicus BRCA2, DNA repair associated (Brca2), transcript variant X4, mRNA.

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CloneID	ORa57847
Clone ID Related Accession (Same CDS sequence)	XM_017598375.1
Accession Version	XM_017598375.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 9216bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	breast cancer type 2 susceptibility protein homolog isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005111.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCGTTG AATACAAAAG GAGACCAACT TTTTGGGAAA TTTTTAAAGC GAGATGCAGC 
ACAGCAGATT TAGGACCAAT AAGCCTCAAT TGGTTTGAAG AACTTTCTTC AGAAGCCCCA 
CCATATAATA CTGAACCTCC AGAAGAATCT GAATATAAAC CCCAAGGCCA TGAACCACAG 
CTATTTAAAA CACCACAGAG GAATCCCTCT TACCATCAGT TTGCTTCAAC TCCAATAATG 
TTCAAAGAAC AAAGTCAAAC TCTACCACTA GACCAGTCAC CTTTCAAAGA ATTAGGGAAT 
GTTGTTGCAA ATAGTAAACG TAAACATCAC AGCAAAAAGA AGGCCAGAAA GGACCCTGTG 
GTAGATGTTG CCAGTCTGCC GCTGAAAGCT TGTCCCAGTG AAAGCCCTTG TACTCCGCGA 
TGCACACAGG TGGCACCGCA GCGAAGAAAG CCAGTGGTAT CTGGAAGTTT ATTCTATACA 
CCAAAACTTG AGGAGACACC AAAACATATT TCTGAAAGTC TGGGAGTTGA AGTGGATCCG 
GATATGTCTT GGACAAGTTC ATTAGCTACA CCACCAACCC TTAGTTCCAC TGTGCTCATA 
GCCCGAGATG AAGAAGCACA CAGAAATGCA TTTCCAGCCG ACTCTCCTGC TAGTTTGAAA 
AGCTATTTTT CTAACCACAA TGAAAGTCTG AAAAAGAATG ATAGATTTAT TCCCTCTGTG 
TCTGACAGTG AAAACAAAAG CCAGCAAGAA GCTTTTAGTC AGGGACTGGA GAAAATGTTA 
GGGGATTCAT CTAGCAAAAT AAATCGCTTC AGAGACTGCC TTAGAAAACC AATACCAAAT 
GTTCTAGAAG ATGGGGAGAC AGCTGTAGAT ACTTCAGGAG AAGATAGTTT CTCGTTATGT 
TTTCCTAAAC GTAGAACCAG AAATCTACAG AAAACGAGAA TGGGCAAGAT GAAGAAGAAA 
ATCTTCAGTG AAACAAGGAC TGATGGATTA AGTGAAGAAG CCAGAGGACA AGCTGATGAT 
AAAAACTCAT TTGCACTTGA AATTGAACCA AGAGATAGTG AGCCCTTAGA TCCATCTGTA 
ACAAACCAGA AGCCCCTTTA CAGCCAGAGT GGGGACATCT CCAGTGAAGC TGGCCAGTGT 
TCAGACAGTA TATGGTCTCA GCCAGATCCC TCTGGTCTAA ATGGAACCCA GACAAGAAAA 
ATACCCCTAC TCCATATTTC TTCTCATAAG CAAAGTATTT TAGAAGACTT CATAGATATG 
AAGAAGGAAG GTACTGGCTC TATTACTTTT CCTCATATTT CTAGTCTTCC AGAACCAGAA 
AAGATGTTCA GTGAGGAAAC TCTGGTAGAC AAGGAACATG AAGGACAGCA TCTTGAGTCA 
CTTGAAGACT CCATCTCAGG GAAACAAATG GTGTCTGGAA CGAGTCAAAC AGCCTGTCTC 
TCTCCGAGTA TCAGGAAGTC TATAGTCAAG ATGAGAGAGC CACTTGAAGA GACTTTGGAT 
ACTGTTTTCT CAGACAGTAT GACCAGCTCA GCCTTTACAG AAGAACTTGA CGCCTCTGCA 
GGGGGACTGG AAATACATAC TGCATGCTCA CAGAGAGAGG ATTCTTTATG TCCTAGTTCA 
GTGGACACTG GAAGCTGGCC AACCACTCTC ACTGACACTT CTGCAACTGT GAAAAATGCA 
GGTTTAATAA CCACTCTCAA AAATAAAAGA AGAAAGTTTA TTTACTCTGT AAGTGATGAT 
GCATCTCATC AAGGAAAAAA ACTACAGACA CAGAGACAGT CAGAGCTTAC TAACCTTTCA 
GCCCCGTTTG AAGCAAGTGC TTTTGAAGTA CCATTCCCCT TTACAAATGT AGATTCAGGT 
ATACCAGATT CTTCAATCAA AAGAAGCAAT TTACCAAATG ATCCTGAAGA GCCATCTTTG 
TCCTTGACCA ACTCTTTTGT GACTGCTGCC AGTAAAGAAA TTAGTTATAT TCATGCATTG 
ATATCTCAGG ATCTAAATGA CAAAGAAGCA ATACTCAGTG AAGAAAAGCC ACAGCCATAT 
ACAGCCCTAG AAGCTGACTT TCTGTCGTGC TTGCCAGAAA GATCATGTGA AAATGATCAA 
AAAAGTCCAA AAGTTTCCGA CCGAAAAGAA AAAGTCTTAG TCTCAGCATG TCGTCCTTCA 
GGAAGGCTTG CAGCAGCAGT GCAGCTCAGC AGCATTAGCT TTGACTCTCA GGAAAACCCT 
CTTGGTAGCC ACAACGTAAC AAGTACTCTT AAATTAACTC CCAGCCCGAA GACACCTCTG 
TCAAAGCCAG TTGTGGTTTC TAGAGGGAAA ATGTGTAAAA TGCCAGAGAA ACTGCAATGT 
AAGAGTTGTA AAGATAATAT TGAATTAAGC AAAAACATCC CTCTGGGGGT TAATGAAATG 
TGTGTCTTAA GTGAAAATTC TGAAACACCT GAGCTTCTGC CACCTCTAGA ATATATAACA 
GAAGTGTCCT CATCAGTGAA GTCACAGTTC AATCAAAATA CAAAAATAGC AGTCGTACAA 
AAGGACCAAA AAGACTCAAC TTTTATTTCA GAAGTAACAG TCCATATGAA TTCTGAAGAA 
CTTTTCCCAG AAAAGGAGAA TAATTTTGCT TTTCAAGTAA CCAATGAAAG CAATAAACCC 
AATATAGGAA GTACTGTGGA ATTCCAGGAA GAAGACCTCA GCCACACAAA AGGGCATAGT 
CTCAAGAACT CTCCCATGAC AGTAGATAGA GACCTAGATG ATGAGCAAGC AGGCCAAGTG 
TTGATTACAG AGGACTCAGA TTCATTAGCA GTAGTCCATG ATTGTACAAA GAAGAGCAGA 
AATACTATAG AGCAGCATCA GAAAGGAACT GCAGACAAAG ACTTCAAGTC AAATTCCTCC 
TTGTATTTGA AATCAGATGG GAACAATGAT TATTTAGACA AATGGTCAGA GTTCTTGGAT 
CCACTCATGA ACCATAAATT TGGAGGTAGC TTCAGAACAG CTTCCAATAA AGAAATAAAA 
CTTTCAGAGG ATAATGTCAA GAAAAGCAAA ATGTTCTTCA AAGATATCGA AGAACAGTAT 
CCTACTAGTT TAGATTGTAT TGACACTGTT AGTACCCTAC AATTAGCAAA CAAGAAGAGA 
CTAAGTGAAC CTCATACATT TGATTTGAAG TCAGGTACTA CTGTATCTAC ACAGTGTCAT 
AGTCAATCAT CTGTTTCTCA TGAAGATACT CACACAGCAC CTCAGATGTT ATCTTCAAAG 
CAAGATTTTC ATTCAAGTCA TAACTTAACG CCCAGCCAAA AAGCAGAAAT TACAGAACTG 
TCTACTATCT TGGAAGAATC AGGAAGTCAG TTTGAATTCA CACAGTTCAA AAATCCAAGC 
CACATAGCAC AGAATAATAC ATCTGCAGTG CTTGGAAACC AGATGGCTGT TGTAAGGACC 
GCTTCTGAGG AGTGGAAAGA TGTTGATCTT CATCTCCCAC TGAATCCCTC CTCTGTAGGT 
CAGATAGATC ACAACAAGAA ATTCGAATGT TTGGTTGGAG TTAAGCAAAG CTCTTCTCAC 
CTGTTAGAAG ACACTTGTAA CCAAAATACA TCTTGTTTTT TACCGATAAA AGAAATGGAG 
TTTGGAGGAT TTTGTTCTGC TCTTGGCACA AAACTTAGTG TGTCTAATGA GGCTCTGAGA 
AAGGCTATGA AACTGTTCAG TGACATTGAA AATATTAGTG AGGAGCCTTC TACAAAAGTA 
GGACCAAGAG GATTCTCTTC ATGTGCACAT CATGATTCTG TTGCTTCCGT GTTTAAGATA 
AAGAAACAAA ACACTGATAA AAGTTTTGAT GAGAAATCTA GTAAGTGCCA GGTAACAGTA 
CAAAATAATA AGGAAATGAC TACCTGTATT CTTGTTGATG AAAATCCTGA AAATTATGTA 
AAGAATATAA AACAAGATAA CAACTATACT GGCTCTCAAA GAAATGCTTA TAAATTAGAA 
AACTCTGATG TTAGTAAATC AAGTACAAGT GGCACAGTTT ATATTAATAA AGGTGACAGT 
GATTTACCTT TTGCTGCTGA AAAAGGCAAT AAGTATCCTG AGTCATGTAC CCAATATGTG 
AGGGAAGAAA ATGCACAAAT TAAGGAAAGT GTATCAGATT TAACATGTTT GGAAGTCATG 
AAAGCTGAGG AAACATGTCA TATGAAATCT TCAGATAAAG AACAATTACC TTCAGATAAG 
ATGGAACAAA ATATGAAAGA GTTTAATATA TCTTTTCAGA CTGCAAGCGG GAAAAATATC 
AGAGTCTCCA AAGAGTCACT AAATAAAAGT GTGAATATTT TAGATCAGGA AACAGAAGAC 
TTGACTGTCA CTTCAGATTC TTTGAATTCT AAAATTCTTT GTGGCATAAA TAAGGACAAA 
ATGCATATTT CATGTCACAA GAAATCAATC AATATTAAAA AGGTATTTGA AGAACATTTC 
CCAATTGGAA CTGTCAGTCA ATTACCAGCT CTTCAGCAGT ATCCTGAATA TGAAATAGAA 
AGTATCAAAG AACCTACTCT GTTGAGTTTT CATACAGCTA GTGGGAAAAA AGTCAAAATT 
ATGCAGGAAT CTTTGGACAA AGTGAAAAAT CTTTTTGATG AGACACAGTA TGTTAGGAAA 
ACCACCAATT TTGGTCATCA AGAATCAAAA CCCCTGAAGG ACAGAGAGGA CTATAAAGAA 
AGACTTACAT TAGCATATGA GAAAATTGAA GTAACTGCCT CAAAATGTGA AGAAATGCAG 
AACTTTGTCT CTAAGCAGAC TGAAATGCTA CCCCAGCAAA ATGATCATAT GTATAGGCAA 
ACTGAAAATC TCACATCAAA TGGTAGCTCT CCCAAAGTAC ATGGAAACAT AGAAAATAAA 
ATAGAAAAGA ATCCTAGAAT TTGCTGTATT TGTCAGTCCT CATACTTTGT CACTGAAGAT 
TCTGCTTTGG CATGTTATAC GGGGGACAGT AGAAAAACTT GTGTCGGAGA GTCTTCTCTG 
TCCAAAGGCA AAAAATGGCT TAGAGAACAA AGTGATAAGC TTGGAACAAG AAATACTATT 
GAAATCCAAT GTGTAAAGGA ACACACTGAA GATTTTGCAG GAAATGCCTT ATATGAACAT 
AGTTTAGTCA TTATCAGAAC TGAAATTGAT ACAAGTCATG TCTCTGAAAA CCAAGCTTCA 
ACCCTCTTTA GTGACCCTAA TGTGTGTCAC AGCTATCTAT CCCATTCTAG TTTTTGTCAT 
CATGATGATA TGCATAATGA TTCAGGATAT TTCTTAAAAG ATAAAATTGA TTCTGATGTC 
CAGCCAGACA TGAAGAATAC TGAAGGCAAT GCCATTTTCC CTAAAATATC TGCTACAAAA 
GAAATAAAAC TACACCCACA AACTGTAAAT GAAGAGTGTG TTCAAAAACT GGAGACTAAT 
GCTTCACCAT ATGCAAATAA AAATATAGCC ATTGACTCAG CTATGCTGGA TTTAAGGAAT 
TGTAAGGTAG GCTCACCTGT ATTCATTACA ACTCATTCAC AAGAAACTGT AAGAATGAAA 
GAGATATTCA CAGATAACTG TAGTAAAATA GTCGAACAAA ACAGGGAGAG TAAACCAGAC 
ACTTGCCAGA CAAGCTGTCA TAAAGCATTG GATAATTCAG AGGATTTTAT ATGTCCTAGC 
TCTTCAGGTG ATGTCTGCAT AAACTCACCT ATGGCTATTT TTTATCCTCA AAGTGAACAA 
ATTTTACAGC ATAACCAAAG TGTGTCTGGA CTGAAGAAAG CTGCAACACC ACCTGTTAGT 
TTGGAAACTT GGGATACATG TAAATCTATA AGAGGATCTC CTCAGGAAGT CCATCCTTCA 
CGCACTTATG GAATTTTTAG CACAGCAAGT GGAAAAGCTG TACAAGTATC TGATGCTTCA 
TTGGAAAAGG CACGGCAAGT GTTTTCTGAG ATAGATGGTG ATGCTAAACA GTTAGCTTCC 
ATGGTGTCAC TGGAAGGTAA TGAAAAATCA CATCACTCTG TGAAAAGAGA AAGCTCTGTG 
GTGCATAACA CCCATGGTGT ATTGTCACTC CGAAAAACCC TCCCAGGCAA TGTCAGTTCA 
TCTGTATTCT CTGGATTTAG CACCGCAGGT GGAAAACTGG TCACAGTTTC AGAAAGTGCT 
TTACATAAAG TTAAAGGAAT GTTAGAGGAA TTTGATTTGA TCAGAACTGA ACATACTCTC 
CAGCATTCAC CTACACCTGA AGACGTATCA AAAATACCTC CTCAACCTTG TCTTGAAAGC 
AGAACCCCAG AATACTCTGT AAGCTCTAAA TTGCAGAAAA CCTACAATGA TAAATCCAGG 
TCACCAAGTA ATTATAAAGA AAGTGGTTCT TCAGGCAATA CTCAATCTCT TGAAGTTTCT 
CCCCAACTCT CTCAGATGGA GAGAAAGCAA GAAACACAGT CGGTATTAGG AACAAAAGTG 
TCCCAGAGGA AGACTAATAT CTTGGAAAAA AAACAAAACT TACCCCAAAA CATAAAAATA 
GAAAGTAATA AAATGGAAAC ATTTTCTGAT GTTTCCATGA AAACAAATGT AGGAGAGTAC 
TACTCCAAAG AGCCAGAGAA CTATTTTGAA ACAGAAGCAG TGGAAATTGC CAAAGCTTTT 
ATGGAAGATG ATGAGCTGAC GGATTCTGAA CAGACTCATG CCAAATGCTC ACTGTTTGCA 
TGCCCCCAAA ACGAGGCTTT ATTAAATTCG AGAACTAGAA AAAGAGGAGG AATGGCTGGT 
GTTGCAGTTG GACAACCCCC AATTAAAAGG AGCTTATTAA ATGAATTTGA CAGGATAATA 
GAAAGTAAAG GAAAATCCTT AACGCCTTCA AAAAGCACTC CAGATGGCAC AATAAAAGAC 
AGACGATTGT TCACACACCA CATGTCTTTA GAGCCGGTTA CCTGTGGACC CTTCTGCTCA 
AGTAAAGAAA GGCAAGAAAC CCAGAGCCCA CATGTTACCT CACCTGCTCA AGGACTTCAG 
TCTAAGGGGC ATCCTTCAAG ACACTCAGCT GTGGGAAAGT CTTCAAGCAA TCCTACAGTT 
TCTGCCCTAA GATCTGAAAG GACCAGACAC TCAGTCTCAG ACAAATCCAC CAAAGTCTTT 
GTCCCACCTT TCAAGGTGAA ATCACGGTTT CACAGAGATG AACATTTTGA TAGCAAGAAT 
GTTAATTTGG AGGGAAAAAA CCAAAAGAGC GCAGATGGAG TCAGTGAAGA TGGGAACGAC 
AGTGACTTTC CTCAGTTTAA CAAAGATTTA ATGTCAAGCC TTCAGAATGC CAGAGACCTA 
CAGGATATAC GAATTAAAAA CAAAGAAAGG CATCATCTCT GTCCGCAGCC AGGCAGTCTG 
TATCTTACAA AATCATCCAC CCTGCCCCGG ATTTCTCTGC AGGCCGCAGT AGGAGACAGC 
GTTCCTTCTG CCTGTTCTCC TAAGCAGCTC TATATGTATG GCGTTTCTAA AGCATGCATA 
AGCGTTAACA GCAAAAACGC TGAGTATTTT CAGTTTGCCA TTGAGGATCA CTTTGGTAAA 
GAAGCTCTGT GCGCTGGCAA AGGCTTTCGG TTGGCAGATG GTGGATGGCT AATCCCCTCC 
GATGACGGGA AGGCTGGGAA AGAAGAATTT TACAGGGCTC TGTGTGACAC TCCAGGTGTG 
GATCCAAAGC TGATTTCTAG CGTCTGGGTC TCTAACCACT ACAGGTGGAT TGTATGGAAA 
CTGGCAGCTA TGGAATTTGC TTTTCCTAAG GAATTCGCGA ATAGATGTCT AAACCCAGAG 
CGAGTGCTGC TTCAACTAAA ATACAGATAC GATGTGGAAA TTGACAATAG CAGCAGATCA 
GCTCTCAAGA AGATACTGGA AAGGGATGAT ACAGCAGCAA AAACGCTTGT CCTCTGTGTT 
TCTGATATCA TTTCACTAAG CACAAATGTG TCTGAAACTT CAGGCAGTAA AGCTAGCAGT 
GAGGACAGTA ACAAAGTAGA CACGATCGAA CTCACAGATG GGTGGTATGC TGTCAAGGCC 
CAGCTAGACC CTCCACTCCT GGCTCTCGTA AAGAGTGGGA GACTCACTGT GGGTCAGAAG 
ATCATTACTC AGGGAGCAGA GCTGGTGGGC TCTCCCGATG CCTGTGCACC TCTGGAAGCC 
CCAGACTCCC TTAGGCTAAA GATTTCTGCA AACAGCACGC GGCCTGCTCG CTGGCACAGC 
AAGCTGGGGT TCTTTCATGA CCCCAGGCCC TTCCCTCTGC CCTTGTCCTC ACTGTTCAGT 
GATGGAGGAA ATGTTGGTTG TGTGGATGTC ATCGTTCAGA GAGTGTACCC TCTACAGTGG 
GTGGAGAAGA CGGTGTCTGG ATCGTACATA TTTCGTAATG AGAGAGAGGA GGAGAAGGAA 
GCGCTGAGAT TTGCAGAGGC CCAGCAGAAG AAACTAGAGG CCTTGTTCAC CAAAGTCCAC 
ACAGAGCTTA AAGAGCATGA AGAAGACATA GCCCAGCGGC GTGTGCTGTC CCGGGCACTC 
ACACGGCAGC AGGTCCACGC TCTGCAGGAC GGTGCAGAGC TCTATGCAGC AGTGCAGGAT 
GCATCAGACC CAGAGCACCT GGAGACTTGT TTCAGCGAAG AGCAGCTGAG AGCCTTGAAC 
AACTACAGAC AAATGCTGAG CGATAAGAAA CAAGCGCGGA TCCAGTCAGA GTTCCGGAAG 
GCCCTGGAGG CCGCTGAGAA AGAAGAGGGT TTATCAAGGG ACGTCTCAAC TGTGTGGAAG 
CTTCGTGTTA CAAGCTACAA GAAAAGAGAA AAATCAGCTC TGTTGAGTAT CTGGCGTCCA 
TCTTCAGACT TGCCCTCCCT GTTAACAGAA GGACAGAGAT ACAGAATCTA TCATCTTTCG 
GTGTCAAAAT CGAAGAATAA ATTTGAGTGG CCCAGCATCC AGTTAACAGC CACAAAGAGA 
ACTCAGTATC AACAGCTGCC GGTTTCCAGT GAGACCCTGC TCCAGCTTTA CCAGCCCAGG 
GAGCTCCTTC CCTTCAGCAA ACTGTCAGAT CCAGCCTTCC AGCCACCTTG TTCTGAAGTG 
GATGTAGTAG GAGTTGTAGT TTCTGTTGTA AAACCAATAG AACATCGACA CATTTTACAA 
GGAGGCAGAA AAGAAGCTTA TACAGGTGCT GAAGGGAGAC AGTCCAAAGT GGTCCACCCC 
GAACAAAGAC CCCACCCGAG AACCCTACCC AGCCTCCACT TGCTCTGCTT CAGACCTTGC 
TTCAGGAGGT CAGTTACCGA GGAGTTCACC TACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017453864.1
CDS	352..9567
Translation	MTVEYKRRPTFWEIFKARCSTADLGPISLNWFEELSSEAPPYNTEPPEESEYKPQGHEPQLFKTPQRNPSYHQFASTPIMFKEQSQTLPLDQSPFKELGNVVANSKRKHHSKKKARKDPVVDVASLPLKACPSESPCTPRCTQVAPQRRKPVVSGSLFYTPKLEETPKHISESLGVEVDPDMSWTSSLATPPTLSSTVLIARDEEAHRNAFPADSPASLKSYFSNHNESLKKNDRFIPSVSDSENKSQQEAFSQGLEKMLGDSSSKINRFRDCLRKPIPNVLEDGETAVDTSGEDSFSLCFPKRRTRNLQKTRMGKMKKKIFSETRTDGLSEEARGQADDKNSFALEIEPRDSEPLDPSVTNQKPLYSQSGDISSEAGQCSDSIWSQPDPSGLNGTQTRKIPLLHISSHKQSILEDFIDMKKEGTGSITFPHISSLPEPEKMFSEETLVDKEHEGQHLESLEDSISGKQMVSGTSQTACLSPSIRKSIVKMREPLEETLDTVFSDSMTSSAFTEELDASAGGLEIHTACSQREDSLCPSSVDTGSWPTTLTDTSATVKNAGLITTLKNKRRKFIYSVSDDASHQGKKLQTQRQSELTNLSAPFEASAFEVPFPFTNVDSGIPDSSIKRSNLPNDPEEPSLSLTNSFVTAASKEISYIHALISQDLNDKEAILSEEKPQPYTALEADFLSCLPERSCENDQKSPKVSDRKEKVLVSACRPSGRLAAAVQLSSISFDSQENPLGSHNVTSTLKLTPSPKTPLSKPVVVSRGKMCKMPEKLQCKSCKDNIELSKNIPLGVNEMCVLSENSETPELLPPLEYITEVSSSVKSQFNQNTKIAVVQKDQKDSTFISEVTVHMNSEELFPEKENNFAFQVTNESNKPNIGSTVEFQEEDLSHTKGHSLKNSPMTVDRDLDDEQAGQVLITEDSDSLAVVHDCTKKSRNTIEQHQKGTADKDFKSNSSLYLKSDGNNDYLDKWSEFLDPLMNHKFGGSFRTASNKEIKLSEDNVKKSKMFFKDIEEQYPTSLDCIDTVSTLQLANKKRLSEPHTFDLKSGTTVSTQCHSQSSVSHEDTHTAPQMLSSKQDFHSSHNLTPSQKAEITELSTILEESGSQFEFTQFKNPSHIAQNNTSAVLGNQMAVVRTASEEWKDVDLHLPLNPSSVGQIDHNKKFECLVGVKQSSSHLLEDTCNQNTSCFLPIKEMEFGGFCSALGTKLSVSNEALRKAMKLFSDIENISEEPSTKVGPRGFSSCAHHDSVASVFKIKKQNTDKSFDEKSSKCQVTVQNNKEMTTCILVDENPENYVKNIKQDNNYTGSQRNAYKLENSDVSKSSTSGTVYINKGDSDLPFAAEKGNKYPESCTQYVREENAQIKESVSDLTCLEVMKAEETCHMKSSDKEQLPSDKMEQNMKEFNISFQTASGKNIRVSKESLNKSVNILDQETEDLTVTSDSLNSKILCGINKDKMHISCHKKSINIKKVFEEHFPIGTVSQLPALQQYPEYEIESIKEPTLLSFHTASGKKVKIMQESLDKVKNLFDETQYVRKTTNFGHQESKPLKDREDYKERLTLAYEKIEVTASKCEEMQNFVSKQTEMLPQQNDHMYRQTENLTSNGSSPKVHGNIENKIEKNPRICCICQSSYFVTEDSALACYTGDSRKTCVGESSLSKGKKWLREQSDKLGTRNTIEIQCVKEHTEDFAGNALYEHSLVIIRTEIDTSHVSENQASTLFSDPNVCHSYLSHSSFCHHDDMHNDSGYFLKDKIDSDVQPDMKNTEGNAIFPKISATKEIKLHPQTVNEECVQKLETNASPYANKNIAIDSAMLDLRNCKVGSPVFITTHSQETVRMKEIFTDNCSKIVEQNRESKPDTCQTSCHKALDNSEDFICPSSSGDVCINSPMAIFYPQSEQILQHNQSVSGLKKAATPPVSLETWDTCKSIRGSPQEVHPSRTYGIFSTASGKAVQVSDASLEKARQVFSEIDGDAKQLASMVSLEGNEKSHHSVKRESSVVHNTHGVLSLRKTLPGNVSSSVFSGFSTAGGKLVTVSESALHKVKGMLEEFDLIRTEHTLQHSPTPEDVSKIPPQPCLESRTPEYSVSSKLQKTYNDKSRSPSNYKESGSSGNTQSLEVSPQLSQMERKQETQSVLGTKVSQRKTNILEKKQNLPQNIKIESNKMETFSDVSMKTNVGEYYSKEPENYFETEAVEIAKAFMEDDELTDSEQTHAKCSLFACPQNEALLNSRTRKRGGMAGVAVGQPPIKRSLLNEFDRIIESKGKSLTPSKSTPDGTIKDRRLFTHHMSLEPVTCGPFCSSKERQETQSPHVTSPAQGLQSKGHPSRHSAVGKSSSNPTVSALRSERTRHSVSDKSTKVFVPPFKVKSRFHRDEHFDSKNVNLEGKNQKSADGVSEDGNDSDFPQFNKDLMSSLQNARDLQDIRIKNKERHHLCPQPGSLYLTKSSTLPRISLQAAVGDSVPSACSPKQLYMYGVSKACISVNSKNAEYFQFAIEDHFGKEALCAGKGFRLADGGWLIPSDDGKAGKEEFYRALCDTPGVDPKLISSVWVSNHYRWIVWKLAAMEFAFPKEFANRCLNPERVLLQLKYRYDVEIDNSSRSALKKILERDDTAAKTLVLCVSDIISLSTNVSETSGSKASSEDSNKVDTIELTDGWYAVKAQLDPPLLALVKSGRLTVGQKIITQGAELVGSPDACAPLEAPDSLRLKISANSTRPARWHSKLGFFHDPRPFPLPLSSLFSDGGNVGCVDVIVQRVYPLQWVEKTVSGSYIFRNEREEEKEALRFAEAQQKKLEALFTKVHTELKEHEEDIAQRRVLSRALTRQQVHALQDGAELYAAVQDASDPEHLETCFSEEQLRALNNYRQMLSDKKQARIQSEFRKALEAAEKEEGLSRDVSTVWKLRVTSYKKREKSALLSIWRPSSDLPSLLTEGQRYRIYHLSVSKSKNKFEWPSIQLTATKRTQYQQLPVSSETLLQLYQPRELLPFSKLSDPAFQPPCSEVDVVGVVVSVVKPIEHRHILQGGRKEAYTGAEGRQSKVVHPEQRPHPRTLPSLHLLCFRPCFRRSVTEEFTY

Target ORF information:

RefSeq Version	XM_017598375.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus BRCA2, DNA repair associated (Brca2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017598375.1

ORF Insert Sequence:

ATGACCGTTG AATACAAAAG GAGACCAACT TTTTGGGAAA TTTTTAAAGC GAGATGCAGC 
ACAGCAGATT TAGGACCAAT AAGCCTCAAT TGGTTTGAAG AACTTTCTTC AGAAGCCCCA 
CCATATAATA CTGAACCTCC AGAAGAATCT GAATATAAAC CCCAAGGCCA TGAACCACAG 
CTATTTAAAA CACCACAGAG GAATCCCTCT TACCATCAGT TTGCTTCAAC TCCAATAATG 
TTCAAAGAAC AAAGTCAAAC TCTACCACTA GACCAGTCAC CTTTCAAAGA ATTAGGGAAT 
GTTGTTGCAA ATAGTAAACG TAAACATCAC AGCAAAAAGA AGGCCAGAAA GGACCCTGTG 
GTAGATGTTG CCAGTCTGCC GCTGAAAGCT TGTCCCAGTG AAAGCCCTTG TACTCCGCGA 
TGCACACAGG TGGCACCGCA GCGAAGAAAG CCAGTGGTAT CTGGAAGTTT ATTCTATACA 
CCAAAACTTG AGGAGACACC AAAACATATT TCTGAAAGTC TGGGAGTTGA AGTGGATCCG 
GATATGTCTT GGACAAGTTC ATTAGCTACA CCACCAACCC TTAGTTCCAC TGTGCTCATA 
GCCCGAGATG AAGAAGCACA CAGAAATGCA TTTCCAGCCG ACTCTCCTGC TAGTTTGAAA 
AGCTATTTTT CTAACCACAA TGAAAGTCTG AAAAAGAATG ATAGATTTAT TCCCTCTGTG 
TCTGACAGTG AAAACAAAAG CCAGCAAGAA GCTTTTAGTC AGGGACTGGA GAAAATGTTA 
GGGGATTCAT CTAGCAAAAT AAATCGCTTC AGAGACTGCC TTAGAAAACC AATACCAAAT 
GTTCTAGAAG ATGGGGAGAC AGCTGTAGAT ACTTCAGGAG AAGATAGTTT CTCGTTATGT 
TTTCCTAAAC GTAGAACCAG AAATCTACAG AAAACGAGAA TGGGCAAGAT GAAGAAGAAA 
ATCTTCAGTG AAACAAGGAC TGATGGATTA AGTGAAGAAG CCAGAGGACA AGCTGATGAT 
AAAAACTCAT TTGCACTTGA AATTGAACCA AGAGATAGTG AGCCCTTAGA TCCATCTGTA 
ACAAACCAGA AGCCCCTTTA CAGCCAGAGT GGGGACATCT CCAGTGAAGC TGGCCAGTGT 
TCAGACAGTA TATGGTCTCA GCCAGATCCC TCTGGTCTAA ATGGAACCCA GACAAGAAAA 
ATACCCCTAC TCCATATTTC TTCTCATAAG CAAAGTATTT TAGAAGACTT CATAGATATG 
AAGAAGGAAG GTACTGGCTC TATTACTTTT CCTCATATTT CTAGTCTTCC AGAACCAGAA 
AAGATGTTCA GTGAGGAAAC TCTGGTAGAC AAGGAACATG AAGGACAGCA TCTTGAGTCA 
CTTGAAGACT CCATCTCAGG GAAACAAATG GTGTCTGGAA CGAGTCAAAC AGCCTGTCTC 
TCTCCGAGTA TCAGGAAGTC TATAGTCAAG ATGAGAGAGC CACTTGAAGA GACTTTGGAT 
ACTGTTTTCT CAGACAGTAT GACCAGCTCA GCCTTTACAG AAGAACTTGA CGCCTCTGCA 
GGGGGACTGG AAATACATAC TGCATGCTCA CAGAGAGAGG ATTCTTTATG TCCTAGTTCA 
GTGGACACTG GAAGCTGGCC AACCACTCTC ACTGACACTT CTGCAACTGT GAAAAATGCA 
GGTTTAATAA CCACTCTCAA AAATAAAAGA AGAAAGTTTA TTTACTCTGT AAGTGATGAT 
GCATCTCATC AAGGAAAAAA ACTACAGACA CAGAGACAGT CAGAGCTTAC TAACCTTTCA 
GCCCCGTTTG AAGCAAGTGC TTTTGAAGTA CCATTCCCCT TTACAAATGT AGATTCAGGT 
ATACCAGATT CTTCAATCAA AAGAAGCAAT TTACCAAATG ATCCTGAAGA GCCATCTTTG 
TCCTTGACCA ACTCTTTTGT GACTGCTGCC AGTAAAGAAA TTAGTTATAT TCATGCATTG 
ATATCTCAGG ATCTAAATGA CAAAGAAGCA ATACTCAGTG AAGAAAAGCC ACAGCCATAT 
ACAGCCCTAG AAGCTGACTT TCTGTCGTGC TTGCCAGAAA GATCATGTGA AAATGATCAA 
AAAAGTCCAA AAGTTTCCGA CCGAAAAGAA AAAGTCTTAG TCTCAGCATG TCGTCCTTCA 
GGAAGGCTTG CAGCAGCAGT GCAGCTCAGC AGCATTAGCT TTGACTCTCA GGAAAACCCT 
CTTGGTAGCC ACAACGTAAC AAGTACTCTT AAATTAACTC CCAGCCCGAA GACACCTCTG 
TCAAAGCCAG TTGTGGTTTC TAGAGGGAAA ATGTGTAAAA TGCCAGAGAA ACTGCAATGT 
AAGAGTTGTA AAGATAATAT TGAATTAAGC AAAAACATCC CTCTGGGGGT TAATGAAATG 
TGTGTCTTAA GTGAAAATTC TGAAACACCT GAGCTTCTGC CACCTCTAGA ATATATAACA 
GAAGTGTCCT CATCAGTGAA GTCACAGTTC AATCAAAATA CAAAAATAGC AGTCGTACAA 
AAGGACCAAA AAGACTCAAC TTTTATTTCA GAAGTAACAG TCCATATGAA TTCTGAAGAA 
CTTTTCCCAG AAAAGGAGAA TAATTTTGCT TTTCAAGTAA CCAATGAAAG CAATAAACCC 
AATATAGGAA GTACTGTGGA ATTCCAGGAA GAAGACCTCA GCCACACAAA AGGGCATAGT 
CTCAAGAACT CTCCCATGAC AGTAGATAGA GACCTAGATG ATGAGCAAGC AGGCCAAGTG 
TTGATTACAG AGGACTCAGA TTCATTAGCA GTAGTCCATG ATTGTACAAA GAAGAGCAGA 
AATACTATAG AGCAGCATCA GAAAGGAACT GCAGACAAAG ACTTCAAGTC AAATTCCTCC 
TTGTATTTGA AATCAGATGG GAACAATGAT TATTTAGACA AATGGTCAGA GTTCTTGGAT 
CCACTCATGA ACCATAAATT TGGAGGTAGC TTCAGAACAG CTTCCAATAA AGAAATAAAA 
CTTTCAGAGG ATAATGTCAA GAAAAGCAAA ATGTTCTTCA AAGATATCGA AGAACAGTAT 
CCTACTAGTT TAGATTGTAT TGACACTGTT AGTACCCTAC AATTAGCAAA CAAGAAGAGA 
CTAAGTGAAC CTCATACATT TGATTTGAAG TCAGGTACTA CTGTATCTAC ACAGTGTCAT 
AGTCAATCAT CTGTTTCTCA TGAAGATACT CACACAGCAC CTCAGATGTT ATCTTCAAAG 
CAAGATTTTC ATTCAAGTCA TAACTTAACG CCCAGCCAAA AAGCAGAAAT TACAGAACTG 
TCTACTATCT TGGAAGAATC AGGAAGTCAG TTTGAATTCA CACAGTTCAA AAATCCAAGC 
CACATAGCAC AGAATAATAC ATCTGCAGTG CTTGGAAACC AGATGGCTGT TGTAAGGACC 
GCTTCTGAGG AGTGGAAAGA TGTTGATCTT CATCTCCCAC TGAATCCCTC CTCTGTAGGT 
CAGATAGATC ACAACAAGAA ATTCGAATGT TTGGTTGGAG TTAAGCAAAG CTCTTCTCAC 
CTGTTAGAAG ACACTTGTAA CCAAAATACA TCTTGTTTTT TACCGATAAA AGAAATGGAG 
TTTGGAGGAT TTTGTTCTGC TCTTGGCACA AAACTTAGTG TGTCTAATGA GGCTCTGAGA 
AAGGCTATGA AACTGTTCAG TGACATTGAA AATATTAGTG AGGAGCCTTC TACAAAAGTA 
GGACCAAGAG GATTCTCTTC ATGTGCACAT CATGATTCTG TTGCTTCCGT GTTTAAGATA 
AAGAAACAAA ACACTGATAA AAGTTTTGAT GAGAAATCTA GTAAGTGCCA GGTAACAGTA 
CAAAATAATA AGGAAATGAC TACCTGTATT CTTGTTGATG AAAATCCTGA AAATTATGTA 
AAGAATATAA AACAAGATAA CAACTATACT GGCTCTCAAA GAAATGCTTA TAAATTAGAA 
AACTCTGATG TTAGTAAATC AAGTACAAGT GGCACAGTTT ATATTAATAA AGGTGACAGT 
GATTTACCTT TTGCTGCTGA AAAAGGCAAT AAGTATCCTG AGTCATGTAC CCAATATGTG 
AGGGAAGAAA ATGCACAAAT TAAGGAAAGT GTATCAGATT TAACATGTTT GGAAGTCATG 
AAAGCTGAGG AAACATGTCA TATGAAATCT TCAGATAAAG AACAATTACC TTCAGATAAG 
ATGGAACAAA ATATGAAAGA GTTTAATATA TCTTTTCAGA CTGCAAGCGG GAAAAATATC 
AGAGTCTCCA AAGAGTCACT AAATAAAAGT GTGAATATTT TAGATCAGGA AACAGAAGAC 
TTGACTGTCA CTTCAGATTC TTTGAATTCT AAAATTCTTT GTGGCATAAA TAAGGACAAA 
ATGCATATTT CATGTCACAA GAAATCAATC AATATTAAAA AGGTATTTGA AGAACATTTC 
CCAATTGGAA CTGTCAGTCA ATTACCAGCT CTTCAGCAGT ATCCTGAATA TGAAATAGAA 
AGTATCAAAG AACCTACTCT GTTGAGTTTT CATACAGCTA GTGGGAAAAA AGTCAAAATT 
ATGCAGGAAT CTTTGGACAA AGTGAAAAAT CTTTTTGATG AGACACAGTA TGTTAGGAAA 
ACCACCAATT TTGGTCATCA AGAATCAAAA CCCCTGAAGG ACAGAGAGGA CTATAAAGAA 
AGACTTACAT TAGCATATGA GAAAATTGAA GTAACTGCCT CAAAATGTGA AGAAATGCAG 
AACTTTGTCT CTAAGCAGAC TGAAATGCTA CCCCAGCAAA ATGATCATAT GTATAGGCAA 
ACTGAAAATC TCACATCAAA TGGTAGCTCT CCCAAAGTAC ATGGAAACAT AGAAAATAAA 
ATAGAAAAGA ATCCTAGAAT TTGCTGTATT TGTCAGTCCT CATACTTTGT CACTGAAGAT 
TCTGCTTTGG CATGTTATAC GGGGGACAGT AGAAAAACTT GTGTCGGAGA GTCTTCTCTG 
TCCAAAGGCA AAAAATGGCT TAGAGAACAA AGTGATAAGC TTGGAACAAG AAATACTATT 
GAAATCCAAT GTGTAAAGGA ACACACTGAA GATTTTGCAG GAAATGCCTT ATATGAACAT 
AGTTTAGTCA TTATCAGAAC TGAAATTGAT ACAAGTCATG TCTCTGAAAA CCAAGCTTCA 
ACCCTCTTTA GTGACCCTAA TGTGTGTCAC AGCTATCTAT CCCATTCTAG TTTTTGTCAT 
CATGATGATA TGCATAATGA TTCAGGATAT TTCTTAAAAG ATAAAATTGA TTCTGATGTC 
CAGCCAGACA TGAAGAATAC TGAAGGCAAT GCCATTTTCC CTAAAATATC TGCTACAAAA 
GAAATAAAAC TACACCCACA AACTGTAAAT GAAGAGTGTG TTCAAAAACT GGAGACTAAT 
GCTTCACCAT ATGCAAATAA AAATATAGCC ATTGACTCAG CTATGCTGGA TTTAAGGAAT 
TGTAAGGTAG GCTCACCTGT ATTCATTACA ACTCATTCAC AAGAAACTGT AAGAATGAAA 
GAGATATTCA CAGATAACTG TAGTAAAATA GTCGAACAAA ACAGGGAGAG TAAACCAGAC 
ACTTGCCAGA CAAGCTGTCA TAAAGCATTG GATAATTCAG AGGATTTTAT ATGTCCTAGC 
TCTTCAGGTG ATGTCTGCAT AAACTCACCT ATGGCTATTT TTTATCCTCA AAGTGAACAA 
ATTTTACAGC ATAACCAAAG TGTGTCTGGA CTGAAGAAAG CTGCAACACC ACCTGTTAGT 
TTGGAAACTT GGGATACATG TAAATCTATA AGAGGATCTC CTCAGGAAGT CCATCCTTCA 
CGCACTTATG GAATTTTTAG CACAGCAAGT GGAAAAGCTG TACAAGTATC TGATGCTTCA 
TTGGAAAAGG CACGGCAAGT GTTTTCTGAG ATAGATGGTG ATGCTAAACA GTTAGCTTCC 
ATGGTGTCAC TGGAAGGTAA TGAAAAATCA CATCACTCTG TGAAAAGAGA AAGCTCTGTG 
GTGCATAACA CCCATGGTGT ATTGTCACTC CGAAAAACCC TCCCAGGCAA TGTCAGTTCA 
TCTGTATTCT CTGGATTTAG CACCGCAGGT GGAAAACTGG TCACAGTTTC AGAAAGTGCT 
TTACATAAAG TTAAAGGAAT GTTAGAGGAA TTTGATTTGA TCAGAACTGA ACATACTCTC 
CAGCATTCAC CTACACCTGA AGACGTATCA AAAATACCTC CTCAACCTTG TCTTGAAAGC 
AGAACCCCAG AATACTCTGT AAGCTCTAAA TTGCAGAAAA CCTACAATGA TAAATCCAGG 
TCACCAAGTA ATTATAAAGA AAGTGGTTCT TCAGGCAATA CTCAATCTCT TGAAGTTTCT 
CCCCAACTCT CTCAGATGGA GAGAAAGCAA GAAACACAGT CGGTATTAGG AACAAAAGTG 
TCCCAGAGGA AGACTAATAT CTTGGAAAAA AAACAAAACT TACCCCAAAA CATAAAAATA 
GAAAGTAATA AAATGGAAAC ATTTTCTGAT GTTTCCATGA AAACAAATGT AGGAGAGTAC 
TACTCCAAAG AGCCAGAGAA CTATTTTGAA ACAGAAGCAG TGGAAATTGC CAAAGCTTTT 
ATGGAAGATG ATGAGCTGAC GGATTCTGAA CAGACTCATG CCAAATGCTC ACTGTTTGCA 
TGCCCCCAAA ACGAGGCTTT ATTAAATTCG AGAACTAGAA AAAGAGGAGG AATGGCTGGT 
GTTGCAGTTG GACAACCCCC AATTAAAAGG AGCTTATTAA ATGAATTTGA CAGGATAATA 
GAAAGTAAAG GAAAATCCTT AACGCCTTCA AAAAGCACTC CAGATGGCAC AATAAAAGAC 
AGACGATTGT TCACACACCA CATGTCTTTA GAGCCGGTTA CCTGTGGACC CTTCTGCTCA 
AGTAAAGAAA GGCAAGAAAC CCAGAGCCCA CATGTTACCT CACCTGCTCA AGGACTTCAG 
TCTAAGGGGC ATCCTTCAAG ACACTCAGCT GTGGGAAAGT CTTCAAGCAA TCCTACAGTT 
TCTGCCCTAA GATCTGAAAG GACCAGACAC TCAGTCTCAG ACAAATCCAC CAAAGTCTTT 
GTCCCACCTT TCAAGGTGAA ATCACGGTTT CACAGAGATG AACATTTTGA TAGCAAGAAT 
GTTAATTTGG AGGGAAAAAA CCAAAAGAGC GCAGATGGAG TCAGTGAAGA TGGGAACGAC 
AGTGACTTTC CTCAGTTTAA CAAAGATTTA ATGTCAAGCC TTCAGAATGC CAGAGACCTA 
CAGGATATAC GAATTAAAAA CAAAGAAAGG CATCATCTCT GTCCGCAGCC AGGCAGTCTG 
TATCTTACAA AATCATCCAC CCTGCCCCGG ATTTCTCTGC AGGCCGCAGT AGGAGACAGC 
GTTCCTTCTG CCTGTTCTCC TAAGCAGCTC TATATGTATG GCGTTTCTAA AGCATGCATA 
AGCGTTAACA GCAAAAACGC TGAGTATTTT CAGTTTGCCA TTGAGGATCA CTTTGGTAAA 
GAAGCTCTGT GCGCTGGCAA AGGCTTTCGG TTGGCAGATG GTGGATGGCT AATCCCCTCC 
GATGACGGGA AGGCTGGGAA AGAAGAATTT TACAGGGCTC TGTGTGACAC TCCAGGTGTG 
GATCCAAAGC TGATTTCTAG CGTCTGGGTC TCTAACCACT ACAGGTGGAT TGTATGGAAA 
CTGGCAGCTA TGGAATTTGC TTTTCCTAAG GAATTCGCGA ATAGATGTCT AAACCCAGAG 
CGAGTGCTGC TTCAACTAAA ATACAGATAC GATGTGGAAA TTGACAATAG CAGCAGATCA 
GCTCTCAAGA AGATACTGGA AAGGGATGAT ACAGCAGCAA AAACGCTTGT CCTCTGTGTT 
TCTGATATCA TTTCACTAAG CACAAATGTG TCTGAAACTT CAGGCAGTAA AGCTAGCAGT 
GAGGACAGTA ACAAAGTAGA CACGATCGAA CTCACAGATG GGTGGTATGC TGTCAAGGCC 
CAGCTAGACC CTCCACTCCT GGCTCTCGTA AAGAGTGGGA GACTCACTGT GGGTCAGAAG 
ATCATTACTC AGGGAGCAGA GCTGGTGGGC TCTCCCGATG CCTGTGCACC TCTGGAAGCC 
CCAGACTCCC TTAGGCTAAA GATTTCTGCA AACAGCACGC GGCCTGCTCG CTGGCACAGC 
AAGCTGGGGT TCTTTCATGA CCCCAGGCCC TTCCCTCTGC CCTTGTCCTC ACTGTTCAGT 
GATGGAGGAA ATGTTGGTTG TGTGGATGTC ATCGTTCAGA GAGTGTACCC TCTACAGTGG 
GTGGAGAAGA CGGTGTCTGG ATCGTACATA TTTCGTAATG AGAGAGAGGA GGAGAAGGAA 
GCGCTGAGAT TTGCAGAGGC CCAGCAGAAG AAACTAGAGG CCTTGTTCAC CAAAGTCCAC 
ACAGAGCTTA AAGAGCATGA AGAAGACATA GCCCAGCGGC GTGTGCTGTC CCGGGCACTC 
ACACGGCAGC AGGTCCACGC TCTGCAGGAC GGTGCAGAGC TCTATGCAGC AGTGCAGGAT 
GCATCAGACC CAGAGCACCT GGAGACTTGT TTCAGCGAAG AGCAGCTGAG AGCCTTGAAC 
AACTACAGAC AAATGCTGAG CGATAAGAAA CAAGCGCGGA TCCAGTCAGA GTTCCGGAAG 
GCCCTGGAGG CCGCTGAGAA AGAAGAGGGT TTATCAAGGG ACGTCTCAAC TGTGTGGAAG 
CTTCGTGTTA CAAGCTACAA GAAAAGAGAA AAATCAGCTC TGTTGAGTAT CTGGCGTCCA 
TCTTCAGACT TGCCCTCCCT GTTAACAGAA GGACAGAGAT ACAGAATCTA TCATCTTTCG 
GTGTCAAAAT CGAAGAATAA ATTTGAGTGG CCCAGCATCC AGTTAACAGC CACAAAGAGA 
ACTCAGTATC AACAGCTGCC GGTTTCCAGT GAGACCCTGC TCCAGCTTTA CCAGCCCAGG 
GAGCTCCTTC CCTTCAGCAA ACTGTCAGAT CCAGCCTTCC AGCCACCTTG TTCTGAAGTG 
GATGTAGTAG GAGTTGTAGT TTCTGTTGTA AAACCAATAG AACATCGACA CATTTTACAA 
GGAGGCAGAA AAGAAGCTTA TACAGGTGCT GAAGGGAGAC AGTCCAAAGT GGTCCACCCC 
GAACAAAGAC CCCACCCGAG AACCCTACCC AGCCTCCACT TGCTCTGCTT CAGACCTTGC 
TTCAGGAGGT CAGTTACCGA GGAGTTCACC TACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genetic variants in fanconi anemia pathway genes BRCA2 and FANCA predict melanoma survival.
The Journal of investigative dermatology135(2)542-550(2015 Feb)
Yin J,Liu H,Liu Z,Wang LE,Chen WV,Zhu D,Amos CI,Fang S,Lee JE,Wei Q

Structure and mechanism of action of the BRCA2 breast cancer tumor suppressor.
Nature structural & molecular biology21(11)962-968(2014 Nov)
Shahid T,Soroka J,Kong E,Malivert L,McIlwraith MJ,Pape T,West SC,Zhang X

Risk of cancer other than breast or ovarian in individuals with BRCA1 and BRCA2 mutations.
Familial cancer11(2)235-42(2012 Jun)
Moran A,O'Hara C,Khan S,Shack L,Woodward E,Maher ER,Lalloo F,Evans DG

p53- and Bax-mediated apoptosis in injured rat spinal cord.
Neurochemical research36(11)2063-74(2011 Nov)
Kotipatruni RR,Dasari VR,Veeravalli KK,Dinh DH,Fassett D,Rao JS

A comprehensive functional characterization of BRCA2 variants associated with Fanconi anemia using mouse ES cell-based assay.
Blood118(9)2430-42(2011 Sep)
Biswas K,Das R,Alter BP,Kuznetsov SG,Stauffer S,North SL,Burkett S,Brody LC,Meyer S,Byrd RA,Sharan SK

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Brca2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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