Phf8 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Phf8
Entrez Gene ID	317425
Full Name	PHD finger protein 8
Synonyms	RGD1561065
General protein information	Preferred Names PHD finger protein 8 Names histone lysine demethylase PHF8
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: X Map Location: Xq12

mRNA and Protein(s)

mRNA	Protein	Name
XM_017602072.1	XP_017457561.1	histone lysine demethylase PHF8 isoform X1
XM_008773119.1	XP_008771341.1	histone lysine demethylase PHF8 isoform X1
XM_006256795.2	XP_006256857.1	histone lysine demethylase PHF8 isoform X2
XM_006256796.2	XP_006256858.1	histone lysine demethylase PHF8 isoform X3
NM_001108253.1	NP_001101723.1	histone lysine demethylase PHF8
XM_006256792.2	XP_006256854.1	histone lysine demethylase PHF8 isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	PHF8	XP_521077.4
Homo sapiens (human)	PHF8	NP_001171825.1
Xenopus tropicalis (tropical clawed frog)	phf8	XP_002939639.1
	PHF8	XP_002808578.1
Canis lupus familiaris (dog)	PHF8	XP_005641445.1
Bos taurus (cattle)	PHF8	XP_005228294.1
Mus musculus (house mouse)	Phf8	NP_001106825.1
Rattus norvegicus (Norway rat)	Phf8	NP_001101723.1
Danio rerio (zebrafish)	phf8	NP_001189376.1

Gene Ontology
Bibliography

Related articles in PubMed

Histone lysine methylation dynamics: establishment, regulation, and biological impact.
Black JC, Van Rechem C, Whetstine JR
Molecular cell48(4)491-507(2012 Nov)

Systematic knockdown of epigenetic enzymes identifies a novel histone demethylase PHF8 overexpressed in prostate cancer with an impact on cell proliferation, migration and invasion.
Bj?rkman M, ?stling P, H?rm? V, Virtanen J, Mpindi JP, Rantala J, Mirtti T, Vesterinen T, Lundin M, Sankila A, Rannikko A, Kaivanto E, Kohonen P, Kallioniemi O, Nees M
Oncogene31(29)3444-56(2012 Jul)

Nucleosome-interacting proteins regulated by DNA and histone methylation.
Bartke T, Vermeulen M, Xhemalce B, Robson SC, Mann M, Kouzarides T
Cell143(3)470-84(2010 Oct)

PHF8 mediates histone H4 lysine 20 demethylation events involved in cell cycle progression.
Liu W, Tanasa B, Tyurina OV, Zhou TY, Gassmann R, Liu WT, Ohgi KA, Benner C, Garcia-Bassets I, Aggarwal AK, Desai A, Dorrestein PC, Glass CK, Rosenfeld MG
Nature466(7305)508-12(2010 Jul)

Histone H4K20/H3K9 demethylase PHF8 regulates zebrafish brain and craniofacial development.
Qi HH, Sarkissian M, Hu GQ, Wang Z, Bhattacharjee A, Gordon DB, Gonzales M, Lan F, Ongusaha PP, Huarte M, Yaghi NK, Lim H, Garcia BA, Brizuela L, Zhao K, Roberts TM, Shi Y
Nature466(7305)503-7(2010 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Phf8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Phf8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa39646	XM_006256795.2 Latest version!	Rattus norvegicus PHD finger protein 8 (Phf8), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
ORa39647	XM_006256796.2 Latest version!	Rattus norvegicus PHD finger protein 8 (Phf8), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
ORa06988	NM_001108253.1 Latest version!	Rattus norvegicus PHD finger protein 8 (Phf8), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
ORa06988	XM_008773119.1 Latest version!	Rattus norvegicus PHD finger protein 8 (Phf8), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
ORa06988	XM_006256792.2 Latest version!	Rattus norvegicus PHD finger protein 8 (Phf8), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
ORa06988	XM_017602072.1 Latest version!	Rattus norvegicus PHD finger protein 8 (Phf8), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Phf8 ( XM_006256795.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006256857.1 Rattus norvegicus PHD finger protein 8 (Phf8), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ORa39646
Clone ID Related Accession (Same CDS sequence)	XM_006256795.2
Accession Version	XM_006256795.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2769bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	histone lysine demethylase PHF8 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005120.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 7, 2014 this sequence version replaced XM_006256795.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCTCGG TGCCTGTGTA TTGCCTCTGT CGACTGCCTT ACGATGTGAC CCGCTTCATG 
ATCGAGTGTG ACATGTGCCA GGACTGGTTT CACGGCAGTT GTGTTGGTGT TGAGGAGGAG 
AAGGCTGCTG ATATTGATCT GTACCATTGC CCCAACTGTG AAGTCTTACA CGGACCCTCC 
ATTATGAAAA AACGTCGTGG ATCTTCCAAA GGACATGATA ATCACAAGGG TAAACCATTG 
AAGACTGGGA GCTCCATGTT TATCCGAGAA CTACGGGGTA GGACTTTTGA CAGCTCAGAC 
GAAGTGATTC TGAAACCCAC TGGAAGTCAG CTGACAGTGG AATTCTTGGA AGAGAATAGC 
TTCAGCGTGC CTATCCTTGT CTTGAAGAAG GATGGCTTGG GGATGACATT ACCCTCTCCA 
TCATTCACTG TGAGGGATGT GGAGCACTAT GTTGGTTCTG ACAAAGAGAT TGATGTGATT 
GATGTGGCCC GCCAGGCTGA CTGCAAAATG AAACTTGGTG ATTTTGTCAA ATACTATTAC 
AGTGGGAAGA GGGAAAAAGT CCTCAATGTC ATTAGTTTGG AATTCTCTGA TACCAGACTT 
TCAAACCTTG TGGAAACACC CAAGATTGTT CGAAAGCTGT CGTGGGTGGA GAACTTGTGG 
CCGGAGGAAT GTGTCTTTGA GAGGCCCAAT GTGCAGAAGT ACTGCCTCAT GAGTGTGCGG 
GATAGCTATA CAGATTTTCA CATTGACTTT GGTGGGACCT CAGTTTGGTA CCATGTGCTT 
AAGGGTGAGA AGATCTTCTA CCTGATCCGC CCAACAAATG CTAATCTGAC TCTCTTTGAG 
TGCTGGAGCA GCTCCTCTAA TCAGAATGAG ATGTTCTTTG GTGACCAGGT GGAAAAATGC 
TACAAGTGTT CTGTGAAGCA AGGACAAACA CTGTTTATTC CTACAGGGTG GATACATGCT 
GTGCTAACAC CAGTGGACTG CTTAGCCTTT GGAGGGAACT TTTTACATAG CCTTAACATT 
GAAATGCAAC TCAAGGCCTA TGAAATTGAG AAGCGGCTGA GCACAGCAGA CCTTTTCAAG 
TTTCCCAACT TCGAGACCAT CTGTTGGTAT GTGGGAAAAC ATATTCTGGA CATCTTCCGA 
GGCTTACGAG AGAACAGGAG ACATCCTGCC TCCTACCTGG TCCATGGTGG TAAAGCTCTG 
AACTTGGCCT TTAGAGCCTG GACAAGGAAA GAAGCTCTGC CAGACCATGA GGATGAGATC 
CCAGAGACAG TGCGGACTGT ACAGCTCATT AAAGATCTGG CTAGGGAGAT CCGCCTGGTC 
GAACTCAACA TTGGTGGTGC CTGCTTGAAT GACTCAGATG ATGACTCAGC AGACATGGAC 
GTTGATGGCA GTGAGAACCC CCTGGCCCTG TTGATGGCTA ATGGCAGTAC AAAGAGGATG 
AAGAGTGTAT CCAAGTCTCG AAGAGCCAAA ATTGCGAAGA AGGTAGACAG TGCAAGACTG 
GTAGCAGAAC AGGTCATGGG GGATGAATTT GACTTGGACT CAGATGATGA GCTGCAGATT 
GATGAGAGAC TGGGAAAGGA AAAAGCGAAC CTGTTAATGA GATCAAAATT TCCCCGGAAA 
TTGCCCCGTG CAAAACCTTG CTCTGACCCC AACCGAATTC GTGAACCTGG AGAAGTTGAG 
TTTGACATTG AGGAGGACTA TACAACAGAT GAGGACATGG TGGAAGGGGT TGAAAGCAAG 
CTTGGTAATG GGAGTGGAGC TGGTGGAATT CTTGATCTAC TTAAGGCCAG CAGGCAGGTC 
GGGGGACCTG ACTATGCTGC CCTCACTGAG GCCCCAGCCT CCCCCAGCAC ACAAGAGGCC 
ATCCAGGGCA TGTTATGTAT GGCCAACCTG CAGTCCTCAT CATCCTCACC AGCTACCTCC 
AGTCTGCAGG CTTGGTGGAC CGGAGGGCAA GACCGAAGCA GTGGGAGCTC CAGCAGTGGG 
CTGGGCACTG TGTCTAATAG TCCTATTTCC CAGCGCACCC CAGGGAAGCG GCCCATCAAG 
AGGCCAGCAT ACTGGAAAAA CGAGAGTGAG GAGGAAGAGA ATGCCAGTAT GGATGAACAA 
GACAGCTTGG GAGCATGCTT CAAGGATGCT GAGTATATCT ATCCATCCCT GGAGTCTGAT 
GATGATGACC CTGCTTTGAA ATCTCGACCC AAGAAAAAGA AGAATTCAGA TGATGCTCCA 
TGGAGTCCTA AAGCCCGTGT GACTCCAACT CTACCCAAGC AGGACCGTCC TGTTCGTGAA 
GGAACCAGAG TTGCCTCCAT TGAGACAGGC TTGGCTGCAG CAGCTGCAAA GCTTGCCCAG 
CAGGAGCTGC AAAAGGCCCA AAAGAAGAAA TATATCAAGA AGAAGCCTTT GCTGAAGGAG 
GTAGAACAGC CTCGCCCTCA AGATTCCAAT ACCACCATGA CAATGCCTGC ACCAACACTG 
GCTACTACAC CCCAGCCTGT CACCTCCTCC TCACCCCAGC CACCTCCTGA GCCTAAACAA 
GAGGCTCTGT CAGGAAGTCT TGCTGACCAT GAGTACACTG CTCGTCCCAA TGCCTTTGGC 
ATGGCTCAGG CAAATCGCAG CACCACACCT ATGGCCCCTG GAGTCTTCCT CACCCAGCGG 
CGCCCTTCAG TTGGTTCCCA GAGCAGTCAG GCAGGACAAG GAAAGCGTCC GAAAAAGGGC 
CTGGCCACAG CAAAGCAAAG ACTCGGCCGC ATCCTGAAAA TCCACAGAAA TGGCAAGTTA 
CTTCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006256857.1
CDS	1069..3837
Translation	MASVPVYCLCRLPYDVTRFMIECDMCQDWFHGSCVGVEEEKAADIDLYHCPNCEVLHGPSIMKKRRGSSKGHDNHKGKPLKTGSSMFIRELRGRTFDSSDEVILKPTGSQLTVEFLEENSFSVPILVLKKDGLGMTLPSPSFTVRDVEHYVGSDKEIDVIDVARQADCKMKLGDFVKYYYSGKREKVLNVISLEFSDTRLSNLVETPKIVRKLSWVENLWPEECVFERPNVQKYCLMSVRDSYTDFHIDFGGTSVWYHVLKGEKIFYLIRPTNANLTLFECWSSSSNQNEMFFGDQVEKCYKCSVKQGQTLFIPTGWIHAVLTPVDCLAFGGNFLHSLNIEMQLKAYEIEKRLSTADLFKFPNFETICWYVGKHILDIFRGLRENRRHPASYLVHGGKALNLAFRAWTRKEALPDHEDEIPETVRTVQLIKDLAREIRLVELNIGGACLNDSDDDSADMDVDGSENPLALLMANGSTKRMKSVSKSRRAKIAKKVDSARLVAEQVMGDEFDLDSDDELQIDERLGKEKANLLMRSKFPRKLPRAKPCSDPNRIREPGEVEFDIEEDYTTDEDMVEGVESKLGNGSGAGGILDLLKASRQVGGPDYAALTEAPASPSTQEAIQGMLCMANLQSSSSSPATSSLQAWWTGGQDRSSGSSSSGLGTVSNSPISQRTPGKRPIKRPAYWKNESEEEENASMDEQDSLGACFKDAEYIYPSLESDDDDPALKSRPKKKKNSDDAPWSPKARVTPTLPKQDRPVREGTRVASIETGLAAAAAKLAQQELQKAQKKKYIKKKPLLKEVEQPRPQDSNTTMTMPAPTLATTPQPVTSSSPQPPPEPKQEALSGSLADHEYTARPNAFGMAQANRSTTPMAPGVFLTQRRPSVGSQSSQAGQGKRPKKGLATAKQRLGRILKIHRNGKLLL

Target ORF information:

RefSeq Version	XM_006256795.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus PHD finger protein 8 (Phf8), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006256795.2

ORF Insert Sequence:

ATGGCCTCGG TGCCTGTGTA TTGCCTCTGT CGACTGCCTT ACGATGTGAC CCGCTTCATG 
ATCGAGTGTG ACATGTGCCA GGACTGGTTT CACGGCAGTT GTGTTGGTGT TGAGGAGGAG 
AAGGCTGCTG ATATTGATCT GTACCATTGC CCCAACTGTG AAGTCTTACA CGGACCCTCC 
ATTATGAAAA AACGTCGTGG ATCTTCCAAA GGACATGATA ATCACAAGGG TAAACCATTG 
AAGACTGGGA GCTCCATGTT TATCCGAGAA CTACGGGGTA GGACTTTTGA CAGCTCAGAC 
GAAGTGATTC TGAAACCCAC TGGAAGTCAG CTGACAGTGG AATTCTTGGA AGAGAATAGC 
TTCAGCGTGC CTATCCTTGT CTTGAAGAAG GATGGCTTGG GGATGACATT ACCCTCTCCA 
TCATTCACTG TGAGGGATGT GGAGCACTAT GTTGGTTCTG ACAAAGAGAT TGATGTGATT 
GATGTGGCCC GCCAGGCTGA CTGCAAAATG AAACTTGGTG ATTTTGTCAA ATACTATTAC 
AGTGGGAAGA GGGAAAAAGT CCTCAATGTC ATTAGTTTGG AATTCTCTGA TACCAGACTT 
TCAAACCTTG TGGAAACACC CAAGATTGTT CGAAAGCTGT CGTGGGTGGA GAACTTGTGG 
CCGGAGGAAT GTGTCTTTGA GAGGCCCAAT GTGCAGAAGT ACTGCCTCAT GAGTGTGCGG 
GATAGCTATA CAGATTTTCA CATTGACTTT GGTGGGACCT CAGTTTGGTA CCATGTGCTT 
AAGGGTGAGA AGATCTTCTA CCTGATCCGC CCAACAAATG CTAATCTGAC TCTCTTTGAG 
TGCTGGAGCA GCTCCTCTAA TCAGAATGAG ATGTTCTTTG GTGACCAGGT GGAAAAATGC 
TACAAGTGTT CTGTGAAGCA AGGACAAACA CTGTTTATTC CTACAGGGTG GATACATGCT 
GTGCTAACAC CAGTGGACTG CTTAGCCTTT GGAGGGAACT TTTTACATAG CCTTAACATT 
GAAATGCAAC TCAAGGCCTA TGAAATTGAG AAGCGGCTGA GCACAGCAGA CCTTTTCAAG 
TTTCCCAACT TCGAGACCAT CTGTTGGTAT GTGGGAAAAC ATATTCTGGA CATCTTCCGA 
GGCTTACGAG AGAACAGGAG ACATCCTGCC TCCTACCTGG TCCATGGTGG TAAAGCTCTG 
AACTTGGCCT TTAGAGCCTG GACAAGGAAA GAAGCTCTGC CAGACCATGA GGATGAGATC 
CCAGAGACAG TGCGGACTGT ACAGCTCATT AAAGATCTGG CTAGGGAGAT CCGCCTGGTC 
GAACTCAACA TTGGTGGTGC CTGCTTGAAT GACTCAGATG ATGACTCAGC AGACATGGAC 
GTTGATGGCA GTGAGAACCC CCTGGCCCTG TTGATGGCTA ATGGCAGTAC AAAGAGGATG 
AAGAGTGTAT CCAAGTCTCG AAGAGCCAAA ATTGCGAAGA AGGTAGACAG TGCAAGACTG 
GTAGCAGAAC AGGTCATGGG GGATGAATTT GACTTGGACT CAGATGATGA GCTGCAGATT 
GATGAGAGAC TGGGAAAGGA AAAAGCGAAC CTGTTAATGA GATCAAAATT TCCCCGGAAA 
TTGCCCCGTG CAAAACCTTG CTCTGACCCC AACCGAATTC GTGAACCTGG AGAAGTTGAG 
TTTGACATTG AGGAGGACTA TACAACAGAT GAGGACATGG TGGAAGGGGT TGAAAGCAAG 
CTTGGTAATG GGAGTGGAGC TGGTGGAATT CTTGATCTAC TTAAGGCCAG CAGGCAGGTC 
GGGGGACCTG ACTATGCTGC CCTCACTGAG GCCCCAGCCT CCCCCAGCAC ACAAGAGGCC 
ATCCAGGGCA TGTTATGTAT GGCCAACCTG CAGTCCTCAT CATCCTCACC AGCTACCTCC 
AGTCTGCAGG CTTGGTGGAC CGGAGGGCAA GACCGAAGCA GTGGGAGCTC CAGCAGTGGG 
CTGGGCACTG TGTCTAATAG TCCTATTTCC CAGCGCACCC CAGGGAAGCG GCCCATCAAG 
AGGCCAGCAT ACTGGAAAAA CGAGAGTGAG GAGGAAGAGA ATGCCAGTAT GGATGAACAA 
GACAGCTTGG GAGCATGCTT CAAGGATGCT GAGTATATCT ATCCATCCCT GGAGTCTGAT 
GATGATGACC CTGCTTTGAA ATCTCGACCC AAGAAAAAGA AGAATTCAGA TGATGCTCCA 
TGGAGTCCTA AAGCCCGTGT GACTCCAACT CTACCCAAGC AGGACCGTCC TGTTCGTGAA 
GGAACCAGAG TTGCCTCCAT TGAGACAGGC TTGGCTGCAG CAGCTGCAAA GCTTGCCCAG 
CAGGAGCTGC AAAAGGCCCA AAAGAAGAAA TATATCAAGA AGAAGCCTTT GCTGAAGGAG 
GTAGAACAGC CTCGCCCTCA AGATTCCAAT ACCACCATGA CAATGCCTGC ACCAACACTG 
GCTACTACAC CCCAGCCTGT CACCTCCTCC TCACCCCAGC CACCTCCTGA GCCTAAACAA 
GAGGCTCTGT CAGGAAGTCT TGCTGACCAT GAGTACACTG CTCGTCCCAA TGCCTTTGGC 
ATGGCTCAGG CAAATCGCAG CACCACACCT ATGGCCCCTG GAGTCTTCCT CACCCAGCGG 
CGCCCTTCAG TTGGTTCCCA GAGCAGTCAG GCAGGACAAG GAAAGCGTCC GAAAAAGGGC 
CTGGCCACAG CAAAGCAAAG ACTCGGCCGC ATCCTGAAAA TCCACAGAAA TGGCAAGTTA 
CTTCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Phf8 ( XM_006256796.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006256858.1 Rattus norvegicus PHD finger protein 8 (Phf8), transcript variant X5, mRNA.

Price & Availability↑

CloneID	ORa39647
Clone ID Related Accession (Same CDS sequence)	XM_006256796.2
Accession Version	XM_006256796.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2760bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	histone lysine demethylase PHF8 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005120.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 7, 2014 this sequence version replaced XM_006256796.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCTCGG TGCCTGTGTA TTGCCTCTGT CGACTGCCTT ACGATGTGAC CCGCTTCATG 
ATCGAGTGTG ACATGTGCCA GGACTGGTTT CACGGCAGTT GTGTTGGTGT TGAGGAGGAG 
AAGGCTGCTG ATATTGATCT GTACCATTGC CCCAACTGTG AAGTCTTACA CGGACCCTCC 
ATTATGAAAA AACGTCGTGG ATCTTCCAAA GGACATGATA ATCACAAGGG TAAACCATTG 
AAGACTGGGA GCTCCATGTT TATCCGAGAA CTACGGGGTA GGACTTTTGA CAGCTCAGAC 
GAAGTGATTC TGAAACCCAC TGGAAGTCAG CTGACAGTGG AATTCTTGGA AGAGAATAGC 
TTCAGCGTGC CTATCCTTGT CTTGAAGAAG GATGGCTTGG GGATGACATT ACCCTCTCCA 
TCATTCACTG TGAGGGATGT GGAGCACTAT GTTGGTTCTG ACAAAGAGAT TGATGTGATT 
GATGTGGCCC GCCAGGCTGA CTGCAAAATG AAACTTGGTG ATTTTGTCAA ATACTATTAC 
AGTGGGAAGA GGGAAAAAGT CCTCAATGTC ATTAGTTTGG AATTCTCTGA TACCAGACTT 
TCAAACCTTG TGGAAACACC CAAGATTGTT CGAAAGCTGT CGTGGGTGGA GAACTTGTGG 
CCGGAGGAAT GTGTCTTTGA GAGGCCCAAT GTGCAGAAGT ACTGCCTCAT GAGTGTGCGG 
GATAGCTATA CAGATTTTCA CATTGACTTT GGTGGGACCT CAGTTTGGTA CCATGTGCTT 
AAGGGTGAGA AGATCTTCTA CCTGATCCGC CCAACAAATG CTAATCTGAC TCTCTTTGAG 
TGCTGGAGCA GCTCCTCTAA TCAGAATGAG ATGTTCTTTG GTGACCAGGT GGAAAAATGC 
TACAAGTGTT CTGTGAAGCA AGGACAAACA CTGTTTATTC CTACAGGGTG GATACATGCT 
GTGCTAACAC CAGTGGACTG CTTAGCCTTT GGAGGGAACT TTTTACATAG CCTTAACATT 
GAAATGCAAC TCAAGGCCTA TGAAATTGAG AAGCGGCTGA GCACAGCAGA CCTTTTCAAG 
TTTCCCAACT TCGAGACCAT CTGTTGGTAT GTGGGAAAAC ATATTCTGGA CATCTTCCGA 
GGCTTACGAG AGAACAGGAG ACATCCTGCC TCCTACCTGG TCCATGGTGG TAAAGCTCTG 
AACTTGGCCT TTAGAGCCTG GACAAGGAAA GAAGCTCTGC CAGACCATGA GGATGAGATC 
CCAGAGACAG TGCGGACTGT ACAGCTCATT AAAGATCTGG CTAGGGAGAT CCGCCTGGTC 
GAAGACATCT TCCAACAGAA CGTTGGGAAG ACGAGCAATA TCTTTGGGCT GCAGAGGATC 
TTCCCAGCTG GTTCCATCCC CTTAACCAGG CCAGCCCATT CCACTTCAGT ATCCATGTCC 
AAGCTGTCAC TGCCCTCCAA AAATGGTTCA AAGAAGAAAG GCCTGAAGCC CAAGGATATC 
TTCAAGAAGG CAGAACGAAA GGGCAAGCAG AGTTCAGCCT TGGGGCCTGC TGGCCAGTTG 
AGCTATAATC TCATGGACCC ATACAGTCAT CAGGCACTGA AGACAGGCCC TTCCCAGAAA 
GCAAAGCTCA ACATTGGTGG TGCCTGCTTG AATGACTCAG ATGATGACTC AGCAGACATG 
GACGTTGATG GCAGTGAGAA CCCCCTGGCC CTGTTGATGG CTAATGGCAG TACAAAGAGG 
ATGAAGAGTG TATCCAAGTC TCGAAGAGCC AAAATTGCGA AGAAGGTAGA CAGTGCAAGA 
CTGGTAGCAG AACAGGTCAT GGGGGATGAA TTTGACTTGG ACTCAGATGA TGAGCTGCAG 
ATTGATGAGA GACTGGGAAA GGAAAAAGCG AACCTGTTAA TGAGATCAAA ATTTCCCCGG 
AAATTGCCCC GTGCAAAACC TTGCTCTGAC CCCAACCGAA TTCGTGAACC TGGAGAAGTT 
GAGTTTGACA TTGAGGAGGA CTATACAACA GATGAGGACA TGGTGGAAGG GGTTGAAAGC 
AAGCTTGGTA ATGGGAGTGG AGCTGGTGGA ATTCTTGATC TACTTAAGGC CAGCAGGCAG 
GTCGGGGGAC CTGACTATGC TGCCCTCACT GAGGCCCCAG CCTCCCCCAG CACACAAGAG 
GCCATCCAGG GCATGTTATG TATGGCCAAC CTGCAGTCCT CATCATCCTC ACCAGCTACC 
TCCAGTCTGC AGGCTTGGTG GACCGGAGGG CAAGACCGAA GCAGTGGGAG CTCCAGCAGT 
GGGCTGGGCA CTGTGTCTAA TAGTCCTATT TCCCAGCGCA CCCCAGGGAA GCGGCCCATC 
AAGAGGCCAG CATACTGGAA AAACGAGAGT GAGGAGGAAG AGAATGCCAG TATGGATGAA 
CAAGACAGCT TGGGAGCATG CTTCAAGGAT GCTGAGTATA TCTATCCATC CCTGGAGTCT 
GATGATGATG ACCCTGCTTT GAAATCTCGA CCCAAGAAAA AGAAGAATTC AGATGATGCT 
CCATGGAGTC CTAAAGCCCG TGTGACTCCA ACTCTACCCA AGCAGGACCG TCCTGTTCGT 
GAAGGAACCA GAGTTGCCTC CATTGAGACA GGCTTGGCTG CAGCAGCTGC AAAGCTTGCC 
CAGCAGGAGC TGCAAAAGGC CCAAAAGAAG AAATATATCA AGAAGAAGCC TTTGCTGAAG 
GAGGAAAGCG TCCGAAAAAG GGCCTGGCCA CAGCAAAGCA AAGACTCGGC CGCATCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006256858.1
CDS	1068..3827
Translation	MASVPVYCLCRLPYDVTRFMIECDMCQDWFHGSCVGVEEEKAADIDLYHCPNCEVLHGPSIMKKRRGSSKGHDNHKGKPLKTGSSMFIRELRGRTFDSSDEVILKPTGSQLTVEFLEENSFSVPILVLKKDGLGMTLPSPSFTVRDVEHYVGSDKEIDVIDVARQADCKMKLGDFVKYYYSGKREKVLNVISLEFSDTRLSNLVETPKIVRKLSWVENLWPEECVFERPNVQKYCLMSVRDSYTDFHIDFGGTSVWYHVLKGEKIFYLIRPTNANLTLFECWSSSSNQNEMFFGDQVEKCYKCSVKQGQTLFIPTGWIHAVLTPVDCLAFGGNFLHSLNIEMQLKAYEIEKRLSTADLFKFPNFETICWYVGKHILDIFRGLRENRRHPASYLVHGGKALNLAFRAWTRKEALPDHEDEIPETVRTVQLIKDLAREIRLVEDIFQQNVGKTSNIFGLQRIFPAGSIPLTRPAHSTSVSMSKLSLPSKNGSKKKGLKPKDIFKKAERKGKQSSALGPAGQLSYNLMDPYSHQALKTGPSQKAKLNIGGACLNDSDDDSADMDVDGSENPLALLMANGSTKRMKSVSKSRRAKIAKKVDSARLVAEQVMGDEFDLDSDDELQIDERLGKEKANLLMRSKFPRKLPRAKPCSDPNRIREPGEVEFDIEEDYTTDEDMVEGVESKLGNGSGAGGILDLLKASRQVGGPDYAALTEAPASPSTQEAIQGMLCMANLQSSSSSPATSSLQAWWTGGQDRSSGSSSSGLGTVSNSPISQRTPGKRPIKRPAYWKNESEEEENASMDEQDSLGACFKDAEYIYPSLESDDDDPALKSRPKKKKNSDDAPWSPKARVTPTLPKQDRPVREGTRVASIETGLAAAAAKLAQQELQKAQKKKYIKKKPLLKEESVRKRAWPQQSKDSAAS

Target ORF information:

RefSeq Version	XM_006256796.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus PHD finger protein 8 (Phf8), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006256796.2

ORF Insert Sequence:

ATGGCCTCGG TGCCTGTGTA TTGCCTCTGT CGACTGCCTT ACGATGTGAC CCGCTTCATG 
ATCGAGTGTG ACATGTGCCA GGACTGGTTT CACGGCAGTT GTGTTGGTGT TGAGGAGGAG 
AAGGCTGCTG ATATTGATCT GTACCATTGC CCCAACTGTG AAGTCTTACA CGGACCCTCC 
ATTATGAAAA AACGTCGTGG ATCTTCCAAA GGACATGATA ATCACAAGGG TAAACCATTG 
AAGACTGGGA GCTCCATGTT TATCCGAGAA CTACGGGGTA GGACTTTTGA CAGCTCAGAC 
GAAGTGATTC TGAAACCCAC TGGAAGTCAG CTGACAGTGG AATTCTTGGA AGAGAATAGC 
TTCAGCGTGC CTATCCTTGT CTTGAAGAAG GATGGCTTGG GGATGACATT ACCCTCTCCA 
TCATTCACTG TGAGGGATGT GGAGCACTAT GTTGGTTCTG ACAAAGAGAT TGATGTGATT 
GATGTGGCCC GCCAGGCTGA CTGCAAAATG AAACTTGGTG ATTTTGTCAA ATACTATTAC 
AGTGGGAAGA GGGAAAAAGT CCTCAATGTC ATTAGTTTGG AATTCTCTGA TACCAGACTT 
TCAAACCTTG TGGAAACACC CAAGATTGTT CGAAAGCTGT CGTGGGTGGA GAACTTGTGG 
CCGGAGGAAT GTGTCTTTGA GAGGCCCAAT GTGCAGAAGT ACTGCCTCAT GAGTGTGCGG 
GATAGCTATA CAGATTTTCA CATTGACTTT GGTGGGACCT CAGTTTGGTA CCATGTGCTT 
AAGGGTGAGA AGATCTTCTA CCTGATCCGC CCAACAAATG CTAATCTGAC TCTCTTTGAG 
TGCTGGAGCA GCTCCTCTAA TCAGAATGAG ATGTTCTTTG GTGACCAGGT GGAAAAATGC 
TACAAGTGTT CTGTGAAGCA AGGACAAACA CTGTTTATTC CTACAGGGTG GATACATGCT 
GTGCTAACAC CAGTGGACTG CTTAGCCTTT GGAGGGAACT TTTTACATAG CCTTAACATT 
GAAATGCAAC TCAAGGCCTA TGAAATTGAG AAGCGGCTGA GCACAGCAGA CCTTTTCAAG 
TTTCCCAACT TCGAGACCAT CTGTTGGTAT GTGGGAAAAC ATATTCTGGA CATCTTCCGA 
GGCTTACGAG AGAACAGGAG ACATCCTGCC TCCTACCTGG TCCATGGTGG TAAAGCTCTG 
AACTTGGCCT TTAGAGCCTG GACAAGGAAA GAAGCTCTGC CAGACCATGA GGATGAGATC 
CCAGAGACAG TGCGGACTGT ACAGCTCATT AAAGATCTGG CTAGGGAGAT CCGCCTGGTC 
GAAGACATCT TCCAACAGAA CGTTGGGAAG ACGAGCAATA TCTTTGGGCT GCAGAGGATC 
TTCCCAGCTG GTTCCATCCC CTTAACCAGG CCAGCCCATT CCACTTCAGT ATCCATGTCC 
AAGCTGTCAC TGCCCTCCAA AAATGGTTCA AAGAAGAAAG GCCTGAAGCC CAAGGATATC 
TTCAAGAAGG CAGAACGAAA GGGCAAGCAG AGTTCAGCCT TGGGGCCTGC TGGCCAGTTG 
AGCTATAATC TCATGGACCC ATACAGTCAT CAGGCACTGA AGACAGGCCC TTCCCAGAAA 
GCAAAGCTCA ACATTGGTGG TGCCTGCTTG AATGACTCAG ATGATGACTC AGCAGACATG 
GACGTTGATG GCAGTGAGAA CCCCCTGGCC CTGTTGATGG CTAATGGCAG TACAAAGAGG 
ATGAAGAGTG TATCCAAGTC TCGAAGAGCC AAAATTGCGA AGAAGGTAGA CAGTGCAAGA 
CTGGTAGCAG AACAGGTCAT GGGGGATGAA TTTGACTTGG ACTCAGATGA TGAGCTGCAG 
ATTGATGAGA GACTGGGAAA GGAAAAAGCG AACCTGTTAA TGAGATCAAA ATTTCCCCGG 
AAATTGCCCC GTGCAAAACC TTGCTCTGAC CCCAACCGAA TTCGTGAACC TGGAGAAGTT 
GAGTTTGACA TTGAGGAGGA CTATACAACA GATGAGGACA TGGTGGAAGG GGTTGAAAGC 
AAGCTTGGTA ATGGGAGTGG AGCTGGTGGA ATTCTTGATC TACTTAAGGC CAGCAGGCAG 
GTCGGGGGAC CTGACTATGC TGCCCTCACT GAGGCCCCAG CCTCCCCCAG CACACAAGAG 
GCCATCCAGG GCATGTTATG TATGGCCAAC CTGCAGTCCT CATCATCCTC ACCAGCTACC 
TCCAGTCTGC AGGCTTGGTG GACCGGAGGG CAAGACCGAA GCAGTGGGAG CTCCAGCAGT 
GGGCTGGGCA CTGTGTCTAA TAGTCCTATT TCCCAGCGCA CCCCAGGGAA GCGGCCCATC 
AAGAGGCCAG CATACTGGAA AAACGAGAGT GAGGAGGAAG AGAATGCCAG TATGGATGAA 
CAAGACAGCT TGGGAGCATG CTTCAAGGAT GCTGAGTATA TCTATCCATC CCTGGAGTCT 
GATGATGATG ACCCTGCTTT GAAATCTCGA CCCAAGAAAA AGAAGAATTC AGATGATGCT 
CCATGGAGTC CTAAAGCCCG TGTGACTCCA ACTCTACCCA AGCAGGACCG TCCTGTTCGT 
GAAGGAACCA GAGTTGCCTC CATTGAGACA GGCTTGGCTG CAGCAGCTGC AAAGCTTGCC 
CAGCAGGAGC TGCAAAAGGC CCAAAAGAAG AAATATATCA AGAAGAAGCC TTTGCTGAAG 
GAGGAAAGCG TCCGAAAAAG GGCCTGGCCA CAGCAAAGCA AAGACTCGGC CGCATCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Phf8 ( NM_001108253.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001101723.1 Rattus norvegicus PHD finger protein 8 (Phf8), mRNA.

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CloneID	ORa06988
Clone ID Related Accession (Same CDS sequence)	XM_017602072.1 , XM_008773119.1 , NM_001108253.1 , XM_006256792.2
Accession Version	NM_001108253.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3072bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Rattus norvegicus(Norway rat)
Product	histone lysine demethylase PHF8
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CH474063.2. On or before Oct 4, 2007 this sequence version replaced XM_001062296.1, XM_001062175.1, XM_001062233.1. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGCCTCGG TGCCTGTGTA TTGCCTCTGT CGACTGCCTT ACGATGTGAC CCGCTTCATG 
ATCGAGTGTG ACATGTGCCA GGACTGGTTT CACGGCAGTT GTGTTGGTGT TGAGGAGGAG 
AAGGCTGCTG ATATTGATCT GTACCATTGC CCCAACTGTG AAGTCTTACA CGGACCCTCC 
ATTATGAAAA AACGTCGTGG ATCTTCCAAA GGACATGATA ATCACAAGGG TAAACCATTG 
AAGACTGGGA GCTCCATGTT TATCCGAGAA CTACGGGGTA GGACTTTTGA CAGCTCAGAC 
GAAGTGATTC TGAAACCCAC TGGAAGTCAG CTGACAGTGG AATTCTTGGA AGAGAATAGC 
TTCAGCGTGC CTATCCTTGT CTTGAAGAAG GATGGCTTGG GGATGACATT ACCCTCTCCA 
TCATTCACTG TGAGGGATGT GGAGCACTAT GTTGGTTCTG ACAAAGAGAT TGATGTGATT 
GATGTGGCCC GCCAGGCTGA CTGCAAAATG AAACTTGGTG ATTTTGTCAA ATACTATTAC 
AGTGGGAAGA GGGAAAAAGT CCTCAATGTC ATTAGTTTGG AATTCTCTGA TACCAGACTT 
TCAAACCTTG TGGAAACACC CAAGATTGTT CGAAAGCTGT CGTGGGTGGA GAACTTGTGG 
CCGGAGGAAT GTGTCTTTGA GAGGCCCAAT GTGCAGAAGT ACTGCCTCAT GAGTGTGCGG 
GATAGCTATA CAGATTTTCA CATTGACTTT GGTGGGACCT CAGTTTGGTA CCATGTGCTT 
AAGGGTGAGA AGATCTTCTA CCTGATCCGC CCAACAAATG CTAATCTGAC TCTCTTTGAG 
TGCTGGAGCA GCTCCTCTAA TCAGAATGAG ATGTTCTTTG GTGACCAGGT GGAAAAATGC 
TACAAGTGTT CTGTGAAGCA AGGACAAACA CTGTTTATTC CTACAGGGTG GATACATGCT 
GTGCTAACAC CAGTGGACTG CTTAGCCTTT GGAGGGAACT TTTTACATAG CCTTAACATT 
GAAATGCAAC TCAAGGCCTA TGAAATTGAG AAGCGGCTGA GCACAGCAGA CCTTTTCAAG 
TTTCCCAACT TCGAGACCAT CTGTTGGTAT GTGGGAAAAC ATATTCTGGA CATCTTCCGA 
GGCTTACGAG AGAACAGGAG ACATCCTGCC TCCTACCTGG TCCATGGTGG TAAAGCTCTG 
AACTTGGCCT TTAGAGCCTG GACAAGGAAA GAAGCTCTGC CAGACCATGA GGATGAGATC 
CCAGAGACAG TGCGGACTGT ACAGCTCATT AAAGATCTGG CTAGGGAGAT CCGCCTGGTC 
GAAGACATCT TCCAACAGAA CGTTGGGAAG ACGAGCAATA TCTTTGGGCT GCAGAGGATC 
TTCCCAGCTG GTTCCATCCC CTTAACCAGG CCAGCCCATT CCACTTCAGT ATCCATGTCC 
AAGCTGTCAC TGCCCTCCAA AAATGGTTCA AAGAAGAAAG GCCTGAAGCC CAAGGATATC 
TTCAAGAAGG CAGAACGAAA GGGCAAGCAG AGTTCAGCCT TGGGGCCTGC TGGCCAGTTG 
AGCTATAATC TCATGGACCC ATACAGTCAT CAGGCACTGA AGACAGGCCC TTCCCAGAAA 
GCAAAGCTCA ACATTGGTGG TGCCTGCTTG AATGACTCAG ATGATGACTC AGCAGACATG 
GACGTTGATG GCAGTGAGAA CCCCCTGGCC CTGTTGATGG CTAATGGCAG TACAAAGAGG 
ATGAAGAGTG TATCCAAGTC TCGAAGAGCC AAAATTGCGA AGAAGGTAGA CAGTGCAAGA 
CTGGTAGCAG AACAGGTCAT GGGGGATGAA TTTGACTTGG ACTCAGATGA TGAGCTGCAG 
ATTGATGAGA GACTGGGAAA GGAAAAAGCG AACCTGTTAA TGAGATCAAA ATTTCCCCGG 
AAATTGCCCC GTGCAAAACC TTGCTCTGAC CCCAACCGAA TTCGTGAACC TGGAGAAGTT 
GAGTTTGACA TTGAGGAGGA CTATACAACA GATGAGGACA TGGTGGAAGG GGTTGAAAGC 
AAGCTTGGTA ATGGGAGTGG AGCTGGTGGA ATTCTTGATC TACTTAAGGC CAGCAGGCAG 
GTCGGGGGAC CTGACTATGC TGCCCTCACT GAGGCCCCAG CCTCCCCCAG CACACAAGAG 
GCCATCCAGG GCATGTTATG TATGGCCAAC CTGCAGTCCT CATCATCCTC ACCAGCTACC 
TCCAGTCTGC AGGCTTGGTG GACCGGAGGG CAAGACCGAA GCAGTGGGAG CTCCAGCAGT 
GGGCTGGGCA CTGTGTCTAA TAGTCCTATT TCCCAGCGCA CCCCAGGGAA GCGGCCCATC 
AAGAGGCCAG CATACTGGAA AAACGAGAGT GAGGAGGAAG AGAATGCCAG TATGGATGAA 
CAAGACAGCT TGGGAGCATG CTTCAAGGAT GCTGAGTATA TCTATCCATC CCTGGAGTCT 
GATGATGATG ACCCTGCTTT GAAATCTCGA CCCAAGAAAA AGAAGAATTC AGATGATGCT 
CCATGGAGTC CTAAAGCCCG TGTGACTCCA ACTCTACCCA AGCAGGACCG TCCTGTTCGT 
GAAGGAACCA GAGTTGCCTC CATTGAGACA GGCTTGGCTG CAGCAGCTGC AAAGCTTGCC 
CAGCAGGAGC TGCAAAAGGC CCAAAAGAAG AAATATATCA AGAAGAAGCC TTTGCTGAAG 
GAGGTAGAAC AGCCTCGCCC TCAAGATTCC AATACCACCA TGACAATGCC TGCACCAACA 
CTGGCTACTA CACCCCAGCC TGTCACCTCC TCCTCACCCC AGCCACCTCC TGAGCCTAAA 
CAAGAGGCTC TGTCAGGAAG TCTTGCTGAC CATGAGTACA CTGCTCGTCC CAATGCCTTT 
GGCATGGCTC AGGCAAATCG CAGCACCACA CCTATGGCCC CTGGAGTCTT CCTCACCCAG 
CGGCGCCCTT CAGTTGGTTC CCAGAGCAGT CAGGCAGGAC AAGGAAAGCG TCCGAAAAAG 
GGCCTGGCCA CAGCAAAGCA AAGACTCGGC CGCATCCTGA AAATCCACAG AAATGGCAAG 
TTACTTCTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001101723.1
CDS	237..3308
Translation	MASVPVYCLCRLPYDVTRFMIECDMCQDWFHGSCVGVEEEKAADIDLYHCPNCEVLHGPSIMKKRRGSSKGHDNHKGKPLKTGSSMFIRELRGRTFDSSDEVILKPTGSQLTVEFLEENSFSVPILVLKKDGLGMTLPSPSFTVRDVEHYVGSDKEIDVIDVARQADCKMKLGDFVKYYYSGKREKVLNVISLEFSDTRLSNLVETPKIVRKLSWVENLWPEECVFERPNVQKYCLMSVRDSYTDFHIDFGGTSVWYHVLKGEKIFYLIRPTNANLTLFECWSSSSNQNEMFFGDQVEKCYKCSVKQGQTLFIPTGWIHAVLTPVDCLAFGGNFLHSLNIEMQLKAYEIEKRLSTADLFKFPNFETICWYVGKHILDIFRGLRENRRHPASYLVHGGKALNLAFRAWTRKEALPDHEDEIPETVRTVQLIKDLAREIRLVEDIFQQNVGKTSNIFGLQRIFPAGSIPLTRPAHSTSVSMSKLSLPSKNGSKKKGLKPKDIFKKAERKGKQSSALGPAGQLSYNLMDPYSHQALKTGPSQKAKLNIGGACLNDSDDDSADMDVDGSENPLALLMANGSTKRMKSVSKSRRAKIAKKVDSARLVAEQVMGDEFDLDSDDELQIDERLGKEKANLLMRSKFPRKLPRAKPCSDPNRIREPGEVEFDIEEDYTTDEDMVEGVESKLGNGSGAGGILDLLKASRQVGGPDYAALTEAPASPSTQEAIQGMLCMANLQSSSSSPATSSLQAWWTGGQDRSSGSSSSGLGTVSNSPISQRTPGKRPIKRPAYWKNESEEEENASMDEQDSLGACFKDAEYIYPSLESDDDDPALKSRPKKKKNSDDAPWSPKARVTPTLPKQDRPVREGTRVASIETGLAAAAAKLAQQELQKAQKKKYIKKKPLLKEVEQPRPQDSNTTMTMPAPTLATTPQPVTSSSPQPPPEPKQEALSGSLADHEYTARPNAFGMAQANRSTTPMAPGVFLTQRRPSVGSQSSQAGQGKRPKKGLATAKQRLGRILKIHRNGKLLL

Target ORF information:

RefSeq Version	NM_001108253.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus PHD finger protein 8 (Phf8), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001108253.1

ORF Insert Sequence:

ATGGCCTCGG TGCCTGTGTA TTGCCTCTGT CGACTGCCTT ACGATGTGAC CCGCTTCATG 
ATCGAGTGTG ACATGTGCCA GGACTGGTTT CACGGCAGTT GTGTTGGTGT TGAGGAGGAG 
AAGGCTGCTG ATATTGATCT GTACCATTGC CCCAACTGTG AAGTCTTACA CGGACCCTCC 
ATTATGAAAA AACGTCGTGG ATCTTCCAAA GGACATGATA ATCACAAGGG TAAACCATTG 
AAGACTGGGA GCTCCATGTT TATCCGAGAA CTACGGGGTA GGACTTTTGA CAGCTCAGAC 
GAAGTGATTC TGAAACCCAC TGGAAGTCAG CTGACAGTGG AATTCTTGGA AGAGAATAGC 
TTCAGCGTGC CTATCCTTGT CTTGAAGAAG GATGGCTTGG GGATGACATT ACCCTCTCCA 
TCATTCACTG TGAGGGATGT GGAGCACTAT GTTGGTTCTG ACAAAGAGAT TGATGTGATT 
GATGTGGCCC GCCAGGCTGA CTGCAAAATG AAACTTGGTG ATTTTGTCAA ATACTATTAC 
AGTGGGAAGA GGGAAAAAGT CCTCAATGTC ATTAGTTTGG AATTCTCTGA TACCAGACTT 
TCAAACCTTG TGGAAACACC CAAGATTGTT CGAAAGCTGT CGTGGGTGGA GAACTTGTGG 
CCGGAGGAAT GTGTCTTTGA GAGGCCCAAT GTGCAGAAGT ACTGCCTCAT GAGTGTGCGG 
GATAGCTATA CAGATTTTCA CATTGACTTT GGTGGGACCT CAGTTTGGTA CCATGTGCTT 
AAGGGTGAGA AGATCTTCTA CCTGATCCGC CCAACAAATG CTAATCTGAC TCTCTTTGAG 
TGCTGGAGCA GCTCCTCTAA TCAGAATGAG ATGTTCTTTG GTGACCAGGT GGAAAAATGC 
TACAAGTGTT CTGTGAAGCA AGGACAAACA CTGTTTATTC CTACAGGGTG GATACATGCT 
GTGCTAACAC CAGTGGACTG CTTAGCCTTT GGAGGGAACT TTTTACATAG CCTTAACATT 
GAAATGCAAC TCAAGGCCTA TGAAATTGAG AAGCGGCTGA GCACAGCAGA CCTTTTCAAG 
TTTCCCAACT TCGAGACCAT CTGTTGGTAT GTGGGAAAAC ATATTCTGGA CATCTTCCGA 
GGCTTACGAG AGAACAGGAG ACATCCTGCC TCCTACCTGG TCCATGGTGG TAAAGCTCTG 
AACTTGGCCT TTAGAGCCTG GACAAGGAAA GAAGCTCTGC CAGACCATGA GGATGAGATC 
CCAGAGACAG TGCGGACTGT ACAGCTCATT AAAGATCTGG CTAGGGAGAT CCGCCTGGTC 
GAAGACATCT TCCAACAGAA CGTTGGGAAG ACGAGCAATA TCTTTGGGCT GCAGAGGATC 
TTCCCAGCTG GTTCCATCCC CTTAACCAGG CCAGCCCATT CCACTTCAGT ATCCATGTCC 
AAGCTGTCAC TGCCCTCCAA AAATGGTTCA AAGAAGAAAG GCCTGAAGCC CAAGGATATC 
TTCAAGAAGG CAGAACGAAA GGGCAAGCAG AGTTCAGCCT TGGGGCCTGC TGGCCAGTTG 
AGCTATAATC TCATGGACCC ATACAGTCAT CAGGCACTGA AGACAGGCCC TTCCCAGAAA 
GCAAAGCTCA ACATTGGTGG TGCCTGCTTG AATGACTCAG ATGATGACTC AGCAGACATG 
GACGTTGATG GCAGTGAGAA CCCCCTGGCC CTGTTGATGG CTAATGGCAG TACAAAGAGG 
ATGAAGAGTG TATCCAAGTC TCGAAGAGCC AAAATTGCGA AGAAGGTAGA CAGTGCAAGA 
CTGGTAGCAG AACAGGTCAT GGGGGATGAA TTTGACTTGG ACTCAGATGA TGAGCTGCAG 
ATTGATGAGA GACTGGGAAA GGAAAAAGCG AACCTGTTAA TGAGATCAAA ATTTCCCCGG 
AAATTGCCCC GTGCAAAACC TTGCTCTGAC CCCAACCGAA TTCGTGAACC TGGAGAAGTT 
GAGTTTGACA TTGAGGAGGA CTATACAACA GATGAGGACA TGGTGGAAGG GGTTGAAAGC 
AAGCTTGGTA ATGGGAGTGG AGCTGGTGGA ATTCTTGATC TACTTAAGGC CAGCAGGCAG 
GTCGGGGGAC CTGACTATGC TGCCCTCACT GAGGCCCCAG CCTCCCCCAG CACACAAGAG 
GCCATCCAGG GCATGTTATG TATGGCCAAC CTGCAGTCCT CATCATCCTC ACCAGCTACC 
TCCAGTCTGC AGGCTTGGTG GACCGGAGGG CAAGACCGAA GCAGTGGGAG CTCCAGCAGT 
GGGCTGGGCA CTGTGTCTAA TAGTCCTATT TCCCAGCGCA CCCCAGGGAA GCGGCCCATC 
AAGAGGCCAG CATACTGGAA AAACGAGAGT GAGGAGGAAG AGAATGCCAG TATGGATGAA 
CAAGACAGCT TGGGAGCATG CTTCAAGGAT GCTGAGTATA TCTATCCATC CCTGGAGTCT 
GATGATGATG ACCCTGCTTT GAAATCTCGA CCCAAGAAAA AGAAGAATTC AGATGATGCT 
CCATGGAGTC CTAAAGCCCG TGTGACTCCA ACTCTACCCA AGCAGGACCG TCCTGTTCGT 
GAAGGAACCA GAGTTGCCTC CATTGAGACA GGCTTGGCTG CAGCAGCTGC AAAGCTTGCC 
CAGCAGGAGC TGCAAAAGGC CCAAAAGAAG AAATATATCA AGAAGAAGCC TTTGCTGAAG 
GAGGTAGAAC AGCCTCGCCC TCAAGATTCC AATACCACCA TGACAATGCC TGCACCAACA 
CTGGCTACTA CACCCCAGCC TGTCACCTCC TCCTCACCCC AGCCACCTCC TGAGCCTAAA 
CAAGAGGCTC TGTCAGGAAG TCTTGCTGAC CATGAGTACA CTGCTCGTCC CAATGCCTTT 
GGCATGGCTC AGGCAAATCG CAGCACCACA CCTATGGCCC CTGGAGTCTT CCTCACCCAG 
CGGCGCCCTT CAGTTGGTTC CCAGAGCAGT CAGGCAGGAC AAGGAAAGCG TCCGAAAAAG 
GGCCTGGCCA CAGCAAAGCA AAGACTCGGC CGCATCCTGA AAATCCACAG AAATGGCAAG 
TTACTTCTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Phf8 ( XM_017602072.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017457561.1 Rattus norvegicus PHD finger protein 8 (Phf8), transcript variant X2, mRNA.

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CloneID	ORa06988
Clone ID Related Accession (Same CDS sequence)	XM_017602072.1 , XM_008773119.1 , NM_001108253.1 , XM_006256792.2
Accession Version	XM_017602072.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3072bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	histone lysine demethylase PHF8 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005120.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCTCGG TGCCTGTGTA TTGCCTCTGT CGACTGCCTT ACGATGTGAC CCGCTTCATG 
ATCGAGTGTG ACATGTGCCA GGACTGGTTT CACGGCAGTT GTGTTGGTGT TGAGGAGGAG 
AAGGCTGCTG ATATTGATCT GTACCATTGC CCCAACTGTG AAGTCTTACA CGGACCCTCC 
ATTATGAAAA AACGTCGTGG ATCTTCCAAA GGACATGATA ATCACAAGGG TAAACCATTG 
AAGACTGGGA GCTCCATGTT TATCCGAGAA CTACGGGGTA GGACTTTTGA CAGCTCAGAC 
GAAGTGATTC TGAAACCCAC TGGAAGTCAG CTGACAGTGG AATTCTTGGA AGAGAATAGC 
TTCAGCGTGC CTATCCTTGT CTTGAAGAAG GATGGCTTGG GGATGACATT ACCCTCTCCA 
TCATTCACTG TGAGGGATGT GGAGCACTAT GTTGGTTCTG ACAAAGAGAT TGATGTGATT 
GATGTGGCCC GCCAGGCTGA CTGCAAAATG AAACTTGGTG ATTTTGTCAA ATACTATTAC 
AGTGGGAAGA GGGAAAAAGT CCTCAATGTC ATTAGTTTGG AATTCTCTGA TACCAGACTT 
TCAAACCTTG TGGAAACACC CAAGATTGTT CGAAAGCTGT CGTGGGTGGA GAACTTGTGG 
CCGGAGGAAT GTGTCTTTGA GAGGCCCAAT GTGCAGAAGT ACTGCCTCAT GAGTGTGCGG 
GATAGCTATA CAGATTTTCA CATTGACTTT GGTGGGACCT CAGTTTGGTA CCATGTGCTT 
AAGGGTGAGA AGATCTTCTA CCTGATCCGC CCAACAAATG CTAATCTGAC TCTCTTTGAG 
TGCTGGAGCA GCTCCTCTAA TCAGAATGAG ATGTTCTTTG GTGACCAGGT GGAAAAATGC 
TACAAGTGTT CTGTGAAGCA AGGACAAACA CTGTTTATTC CTACAGGGTG GATACATGCT 
GTGCTAACAC CAGTGGACTG CTTAGCCTTT GGAGGGAACT TTTTACATAG CCTTAACATT 
GAAATGCAAC TCAAGGCCTA TGAAATTGAG AAGCGGCTGA GCACAGCAGA CCTTTTCAAG 
TTTCCCAACT TCGAGACCAT CTGTTGGTAT GTGGGAAAAC ATATTCTGGA CATCTTCCGA 
GGCTTACGAG AGAACAGGAG ACATCCTGCC TCCTACCTGG TCCATGGTGG TAAAGCTCTG 
AACTTGGCCT TTAGAGCCTG GACAAGGAAA GAAGCTCTGC CAGACCATGA GGATGAGATC 
CCAGAGACAG TGCGGACTGT ACAGCTCATT AAAGATCTGG CTAGGGAGAT CCGCCTGGTC 
GAAGACATCT TCCAACAGAA CGTTGGGAAG ACGAGCAATA TCTTTGGGCT GCAGAGGATC 
TTCCCAGCTG GTTCCATCCC CTTAACCAGG CCAGCCCATT CCACTTCAGT ATCCATGTCC 
AAGCTGTCAC TGCCCTCCAA AAATGGTTCA AAGAAGAAAG GCCTGAAGCC CAAGGATATC 
TTCAAGAAGG CAGAACGAAA GGGCAAGCAG AGTTCAGCCT TGGGGCCTGC TGGCCAGTTG 
AGCTATAATC TCATGGACCC ATACAGTCAT CAGGCACTGA AGACAGGCCC TTCCCAGAAA 
GCAAAGCTCA ACATTGGTGG TGCCTGCTTG AATGACTCAG ATGATGACTC AGCAGACATG 
GACGTTGATG GCAGTGAGAA CCCCCTGGCC CTGTTGATGG CTAATGGCAG TACAAAGAGG 
ATGAAGAGTG TATCCAAGTC TCGAAGAGCC AAAATTGCGA AGAAGGTAGA CAGTGCAAGA 
CTGGTAGCAG AACAGGTCAT GGGGGATGAA TTTGACTTGG ACTCAGATGA TGAGCTGCAG 
ATTGATGAGA GACTGGGAAA GGAAAAAGCG AACCTGTTAA TGAGATCAAA ATTTCCCCGG 
AAATTGCCCC GTGCAAAACC TTGCTCTGAC CCCAACCGAA TTCGTGAACC TGGAGAAGTT 
GAGTTTGACA TTGAGGAGGA CTATACAACA GATGAGGACA TGGTGGAAGG GGTTGAAAGC 
AAGCTTGGTA ATGGGAGTGG AGCTGGTGGA ATTCTTGATC TACTTAAGGC CAGCAGGCAG 
GTCGGGGGAC CTGACTATGC TGCCCTCACT GAGGCCCCAG CCTCCCCCAG CACACAAGAG 
GCCATCCAGG GCATGTTATG TATGGCCAAC CTGCAGTCCT CATCATCCTC ACCAGCTACC 
TCCAGTCTGC AGGCTTGGTG GACCGGAGGG CAAGACCGAA GCAGTGGGAG CTCCAGCAGT 
GGGCTGGGCA CTGTGTCTAA TAGTCCTATT TCCCAGCGCA CCCCAGGGAA GCGGCCCATC 
AAGAGGCCAG CATACTGGAA AAACGAGAGT GAGGAGGAAG AGAATGCCAG TATGGATGAA 
CAAGACAGCT TGGGAGCATG CTTCAAGGAT GCTGAGTATA TCTATCCATC CCTGGAGTCT 
GATGATGATG ACCCTGCTTT GAAATCTCGA CCCAAGAAAA AGAAGAATTC AGATGATGCT 
CCATGGAGTC CTAAAGCCCG TGTGACTCCA ACTCTACCCA AGCAGGACCG TCCTGTTCGT 
GAAGGAACCA GAGTTGCCTC CATTGAGACA GGCTTGGCTG CAGCAGCTGC AAAGCTTGCC 
CAGCAGGAGC TGCAAAAGGC CCAAAAGAAG AAATATATCA AGAAGAAGCC TTTGCTGAAG 
GAGGTAGAAC AGCCTCGCCC TCAAGATTCC AATACCACCA TGACAATGCC TGCACCAACA 
CTGGCTACTA CACCCCAGCC TGTCACCTCC TCCTCACCCC AGCCACCTCC TGAGCCTAAA 
CAAGAGGCTC TGTCAGGAAG TCTTGCTGAC CATGAGTACA CTGCTCGTCC CAATGCCTTT 
GGCATGGCTC AGGCAAATCG CAGCACCACA CCTATGGCCC CTGGAGTCTT CCTCACCCAG 
CGGCGCCCTT CAGTTGGTTC CCAGAGCAGT CAGGCAGGAC AAGGAAAGCG TCCGAAAAAG 
GGCCTGGCCA CAGCAAAGCA AAGACTCGGC CGCATCCTGA AAATCCACAG AAATGGCAAG 
TTACTTCTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017457561.1
CDS	347..3418
Translation	MASVPVYCLCRLPYDVTRFMIECDMCQDWFHGSCVGVEEEKAADIDLYHCPNCEVLHGPSIMKKRRGSSKGHDNHKGKPLKTGSSMFIRELRGRTFDSSDEVILKPTGSQLTVEFLEENSFSVPILVLKKDGLGMTLPSPSFTVRDVEHYVGSDKEIDVIDVARQADCKMKLGDFVKYYYSGKREKVLNVISLEFSDTRLSNLVETPKIVRKLSWVENLWPEECVFERPNVQKYCLMSVRDSYTDFHIDFGGTSVWYHVLKGEKIFYLIRPTNANLTLFECWSSSSNQNEMFFGDQVEKCYKCSVKQGQTLFIPTGWIHAVLTPVDCLAFGGNFLHSLNIEMQLKAYEIEKRLSTADLFKFPNFETICWYVGKHILDIFRGLRENRRHPASYLVHGGKALNLAFRAWTRKEALPDHEDEIPETVRTVQLIKDLAREIRLVEDIFQQNVGKTSNIFGLQRIFPAGSIPLTRPAHSTSVSMSKLSLPSKNGSKKKGLKPKDIFKKAERKGKQSSALGPAGQLSYNLMDPYSHQALKTGPSQKAKLNIGGACLNDSDDDSADMDVDGSENPLALLMANGSTKRMKSVSKSRRAKIAKKVDSARLVAEQVMGDEFDLDSDDELQIDERLGKEKANLLMRSKFPRKLPRAKPCSDPNRIREPGEVEFDIEEDYTTDEDMVEGVESKLGNGSGAGGILDLLKASRQVGGPDYAALTEAPASPSTQEAIQGMLCMANLQSSSSSPATSSLQAWWTGGQDRSSGSSSSGLGTVSNSPISQRTPGKRPIKRPAYWKNESEEEENASMDEQDSLGACFKDAEYIYPSLESDDDDPALKSRPKKKKNSDDAPWSPKARVTPTLPKQDRPVREGTRVASIETGLAAAAAKLAQQELQKAQKKKYIKKKPLLKEVEQPRPQDSNTTMTMPAPTLATTPQPVTSSSPQPPPEPKQEALSGSLADHEYTARPNAFGMAQANRSTTPMAPGVFLTQRRPSVGSQSSQAGQGKRPKKGLATAKQRLGRILKIHRNGKLLL

Target ORF information:

RefSeq Version	XM_017602072.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus PHD finger protein 8 (Phf8), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017602072.1

ORF Insert Sequence:

ATGGCCTCGG TGCCTGTGTA TTGCCTCTGT CGACTGCCTT ACGATGTGAC CCGCTTCATG 
ATCGAGTGTG ACATGTGCCA GGACTGGTTT CACGGCAGTT GTGTTGGTGT TGAGGAGGAG 
AAGGCTGCTG ATATTGATCT GTACCATTGC CCCAACTGTG AAGTCTTACA CGGACCCTCC 
ATTATGAAAA AACGTCGTGG ATCTTCCAAA GGACATGATA ATCACAAGGG TAAACCATTG 
AAGACTGGGA GCTCCATGTT TATCCGAGAA CTACGGGGTA GGACTTTTGA CAGCTCAGAC 
GAAGTGATTC TGAAACCCAC TGGAAGTCAG CTGACAGTGG AATTCTTGGA AGAGAATAGC 
TTCAGCGTGC CTATCCTTGT CTTGAAGAAG GATGGCTTGG GGATGACATT ACCCTCTCCA 
TCATTCACTG TGAGGGATGT GGAGCACTAT GTTGGTTCTG ACAAAGAGAT TGATGTGATT 
GATGTGGCCC GCCAGGCTGA CTGCAAAATG AAACTTGGTG ATTTTGTCAA ATACTATTAC 
AGTGGGAAGA GGGAAAAAGT CCTCAATGTC ATTAGTTTGG AATTCTCTGA TACCAGACTT 
TCAAACCTTG TGGAAACACC CAAGATTGTT CGAAAGCTGT CGTGGGTGGA GAACTTGTGG 
CCGGAGGAAT GTGTCTTTGA GAGGCCCAAT GTGCAGAAGT ACTGCCTCAT GAGTGTGCGG 
GATAGCTATA CAGATTTTCA CATTGACTTT GGTGGGACCT CAGTTTGGTA CCATGTGCTT 
AAGGGTGAGA AGATCTTCTA CCTGATCCGC CCAACAAATG CTAATCTGAC TCTCTTTGAG 
TGCTGGAGCA GCTCCTCTAA TCAGAATGAG ATGTTCTTTG GTGACCAGGT GGAAAAATGC 
TACAAGTGTT CTGTGAAGCA AGGACAAACA CTGTTTATTC CTACAGGGTG GATACATGCT 
GTGCTAACAC CAGTGGACTG CTTAGCCTTT GGAGGGAACT TTTTACATAG CCTTAACATT 
GAAATGCAAC TCAAGGCCTA TGAAATTGAG AAGCGGCTGA GCACAGCAGA CCTTTTCAAG 
TTTCCCAACT TCGAGACCAT CTGTTGGTAT GTGGGAAAAC ATATTCTGGA CATCTTCCGA 
GGCTTACGAG AGAACAGGAG ACATCCTGCC TCCTACCTGG TCCATGGTGG TAAAGCTCTG 
AACTTGGCCT TTAGAGCCTG GACAAGGAAA GAAGCTCTGC CAGACCATGA GGATGAGATC 
CCAGAGACAG TGCGGACTGT ACAGCTCATT AAAGATCTGG CTAGGGAGAT CCGCCTGGTC 
GAAGACATCT TCCAACAGAA CGTTGGGAAG ACGAGCAATA TCTTTGGGCT GCAGAGGATC 
TTCCCAGCTG GTTCCATCCC CTTAACCAGG CCAGCCCATT CCACTTCAGT ATCCATGTCC 
AAGCTGTCAC TGCCCTCCAA AAATGGTTCA AAGAAGAAAG GCCTGAAGCC CAAGGATATC 
TTCAAGAAGG CAGAACGAAA GGGCAAGCAG AGTTCAGCCT TGGGGCCTGC TGGCCAGTTG 
AGCTATAATC TCATGGACCC ATACAGTCAT CAGGCACTGA AGACAGGCCC TTCCCAGAAA 
GCAAAGCTCA ACATTGGTGG TGCCTGCTTG AATGACTCAG ATGATGACTC AGCAGACATG 
GACGTTGATG GCAGTGAGAA CCCCCTGGCC CTGTTGATGG CTAATGGCAG TACAAAGAGG 
ATGAAGAGTG TATCCAAGTC TCGAAGAGCC AAAATTGCGA AGAAGGTAGA CAGTGCAAGA 
CTGGTAGCAG AACAGGTCAT GGGGGATGAA TTTGACTTGG ACTCAGATGA TGAGCTGCAG 
ATTGATGAGA GACTGGGAAA GGAAAAAGCG AACCTGTTAA TGAGATCAAA ATTTCCCCGG 
AAATTGCCCC GTGCAAAACC TTGCTCTGAC CCCAACCGAA TTCGTGAACC TGGAGAAGTT 
GAGTTTGACA TTGAGGAGGA CTATACAACA GATGAGGACA TGGTGGAAGG GGTTGAAAGC 
AAGCTTGGTA ATGGGAGTGG AGCTGGTGGA ATTCTTGATC TACTTAAGGC CAGCAGGCAG 
GTCGGGGGAC CTGACTATGC TGCCCTCACT GAGGCCCCAG CCTCCCCCAG CACACAAGAG 
GCCATCCAGG GCATGTTATG TATGGCCAAC CTGCAGTCCT CATCATCCTC ACCAGCTACC 
TCCAGTCTGC AGGCTTGGTG GACCGGAGGG CAAGACCGAA GCAGTGGGAG CTCCAGCAGT 
GGGCTGGGCA CTGTGTCTAA TAGTCCTATT TCCCAGCGCA CCCCAGGGAA GCGGCCCATC 
AAGAGGCCAG CATACTGGAA AAACGAGAGT GAGGAGGAAG AGAATGCCAG TATGGATGAA 
CAAGACAGCT TGGGAGCATG CTTCAAGGAT GCTGAGTATA TCTATCCATC CCTGGAGTCT 
GATGATGATG ACCCTGCTTT GAAATCTCGA CCCAAGAAAA AGAAGAATTC AGATGATGCT 
CCATGGAGTC CTAAAGCCCG TGTGACTCCA ACTCTACCCA AGCAGGACCG TCCTGTTCGT 
GAAGGAACCA GAGTTGCCTC CATTGAGACA GGCTTGGCTG CAGCAGCTGC AAAGCTTGCC 
CAGCAGGAGC TGCAAAAGGC CCAAAAGAAG AAATATATCA AGAAGAAGCC TTTGCTGAAG 
GAGGTAGAAC AGCCTCGCCC TCAAGATTCC AATACCACCA TGACAATGCC TGCACCAACA 
CTGGCTACTA CACCCCAGCC TGTCACCTCC TCCTCACCCC AGCCACCTCC TGAGCCTAAA 
CAAGAGGCTC TGTCAGGAAG TCTTGCTGAC CATGAGTACA CTGCTCGTCC CAATGCCTTT 
GGCATGGCTC AGGCAAATCG CAGCACCACA CCTATGGCCC CTGGAGTCTT CCTCACCCAG 
CGGCGCCCTT CAGTTGGTTC CCAGAGCAGT CAGGCAGGAC AAGGAAAGCG TCCGAAAAAG 
GGCCTGGCCA CAGCAAAGCA AAGACTCGGC CGCATCCTGA AAATCCACAG AAATGGCAAG 
TTACTTCTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Phf8 ( XM_008773119.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008771341.1 Rattus norvegicus PHD finger protein 8 (Phf8), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ORa06988
Clone ID Related Accession (Same CDS sequence)	XM_017602072.1 , XM_008773119.1 , NM_001108253.1 , XM_006256792.2
Accession Version	XM_008773119.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3072bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	histone lysine demethylase PHF8 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005120.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCTCGG TGCCTGTGTA TTGCCTCTGT CGACTGCCTT ACGATGTGAC CCGCTTCATG 
ATCGAGTGTG ACATGTGCCA GGACTGGTTT CACGGCAGTT GTGTTGGTGT TGAGGAGGAG 
AAGGCTGCTG ATATTGATCT GTACCATTGC CCCAACTGTG AAGTCTTACA CGGACCCTCC 
ATTATGAAAA AACGTCGTGG ATCTTCCAAA GGACATGATA ATCACAAGGG TAAACCATTG 
AAGACTGGGA GCTCCATGTT TATCCGAGAA CTACGGGGTA GGACTTTTGA CAGCTCAGAC 
GAAGTGATTC TGAAACCCAC TGGAAGTCAG CTGACAGTGG AATTCTTGGA AGAGAATAGC 
TTCAGCGTGC CTATCCTTGT CTTGAAGAAG GATGGCTTGG GGATGACATT ACCCTCTCCA 
TCATTCACTG TGAGGGATGT GGAGCACTAT GTTGGTTCTG ACAAAGAGAT TGATGTGATT 
GATGTGGCCC GCCAGGCTGA CTGCAAAATG AAACTTGGTG ATTTTGTCAA ATACTATTAC 
AGTGGGAAGA GGGAAAAAGT CCTCAATGTC ATTAGTTTGG AATTCTCTGA TACCAGACTT 
TCAAACCTTG TGGAAACACC CAAGATTGTT CGAAAGCTGT CGTGGGTGGA GAACTTGTGG 
CCGGAGGAAT GTGTCTTTGA GAGGCCCAAT GTGCAGAAGT ACTGCCTCAT GAGTGTGCGG 
GATAGCTATA CAGATTTTCA CATTGACTTT GGTGGGACCT CAGTTTGGTA CCATGTGCTT 
AAGGGTGAGA AGATCTTCTA CCTGATCCGC CCAACAAATG CTAATCTGAC TCTCTTTGAG 
TGCTGGAGCA GCTCCTCTAA TCAGAATGAG ATGTTCTTTG GTGACCAGGT GGAAAAATGC 
TACAAGTGTT CTGTGAAGCA AGGACAAACA CTGTTTATTC CTACAGGGTG GATACATGCT 
GTGCTAACAC CAGTGGACTG CTTAGCCTTT GGAGGGAACT TTTTACATAG CCTTAACATT 
GAAATGCAAC TCAAGGCCTA TGAAATTGAG AAGCGGCTGA GCACAGCAGA CCTTTTCAAG 
TTTCCCAACT TCGAGACCAT CTGTTGGTAT GTGGGAAAAC ATATTCTGGA CATCTTCCGA 
GGCTTACGAG AGAACAGGAG ACATCCTGCC TCCTACCTGG TCCATGGTGG TAAAGCTCTG 
AACTTGGCCT TTAGAGCCTG GACAAGGAAA GAAGCTCTGC CAGACCATGA GGATGAGATC 
CCAGAGACAG TGCGGACTGT ACAGCTCATT AAAGATCTGG CTAGGGAGAT CCGCCTGGTC 
GAAGACATCT TCCAACAGAA CGTTGGGAAG ACGAGCAATA TCTTTGGGCT GCAGAGGATC 
TTCCCAGCTG GTTCCATCCC CTTAACCAGG CCAGCCCATT CCACTTCAGT ATCCATGTCC 
AAGCTGTCAC TGCCCTCCAA AAATGGTTCA AAGAAGAAAG GCCTGAAGCC CAAGGATATC 
TTCAAGAAGG CAGAACGAAA GGGCAAGCAG AGTTCAGCCT TGGGGCCTGC TGGCCAGTTG 
AGCTATAATC TCATGGACCC ATACAGTCAT CAGGCACTGA AGACAGGCCC TTCCCAGAAA 
GCAAAGCTCA ACATTGGTGG TGCCTGCTTG AATGACTCAG ATGATGACTC AGCAGACATG 
GACGTTGATG GCAGTGAGAA CCCCCTGGCC CTGTTGATGG CTAATGGCAG TACAAAGAGG 
ATGAAGAGTG TATCCAAGTC TCGAAGAGCC AAAATTGCGA AGAAGGTAGA CAGTGCAAGA 
CTGGTAGCAG AACAGGTCAT GGGGGATGAA TTTGACTTGG ACTCAGATGA TGAGCTGCAG 
ATTGATGAGA GACTGGGAAA GGAAAAAGCG AACCTGTTAA TGAGATCAAA ATTTCCCCGG 
AAATTGCCCC GTGCAAAACC TTGCTCTGAC CCCAACCGAA TTCGTGAACC TGGAGAAGTT 
GAGTTTGACA TTGAGGAGGA CTATACAACA GATGAGGACA TGGTGGAAGG GGTTGAAAGC 
AAGCTTGGTA ATGGGAGTGG AGCTGGTGGA ATTCTTGATC TACTTAAGGC CAGCAGGCAG 
GTCGGGGGAC CTGACTATGC TGCCCTCACT GAGGCCCCAG CCTCCCCCAG CACACAAGAG 
GCCATCCAGG GCATGTTATG TATGGCCAAC CTGCAGTCCT CATCATCCTC ACCAGCTACC 
TCCAGTCTGC AGGCTTGGTG GACCGGAGGG CAAGACCGAA GCAGTGGGAG CTCCAGCAGT 
GGGCTGGGCA CTGTGTCTAA TAGTCCTATT TCCCAGCGCA CCCCAGGGAA GCGGCCCATC 
AAGAGGCCAG CATACTGGAA AAACGAGAGT GAGGAGGAAG AGAATGCCAG TATGGATGAA 
CAAGACAGCT TGGGAGCATG CTTCAAGGAT GCTGAGTATA TCTATCCATC CCTGGAGTCT 
GATGATGATG ACCCTGCTTT GAAATCTCGA CCCAAGAAAA AGAAGAATTC AGATGATGCT 
CCATGGAGTC CTAAAGCCCG TGTGACTCCA ACTCTACCCA AGCAGGACCG TCCTGTTCGT 
GAAGGAACCA GAGTTGCCTC CATTGAGACA GGCTTGGCTG CAGCAGCTGC AAAGCTTGCC 
CAGCAGGAGC TGCAAAAGGC CCAAAAGAAG AAATATATCA AGAAGAAGCC TTTGCTGAAG 
GAGGTAGAAC AGCCTCGCCC TCAAGATTCC AATACCACCA TGACAATGCC TGCACCAACA 
CTGGCTACTA CACCCCAGCC TGTCACCTCC TCCTCACCCC AGCCACCTCC TGAGCCTAAA 
CAAGAGGCTC TGTCAGGAAG TCTTGCTGAC CATGAGTACA CTGCTCGTCC CAATGCCTTT 
GGCATGGCTC AGGCAAATCG CAGCACCACA CCTATGGCCC CTGGAGTCTT CCTCACCCAG 
CGGCGCCCTT CAGTTGGTTC CCAGAGCAGT CAGGCAGGAC AAGGAAAGCG TCCGAAAAAG 
GGCCTGGCCA CAGCAAAGCA AAGACTCGGC CGCATCCTGA AAATCCACAG AAATGGCAAG 
TTACTTCTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008771341.1
CDS	207..3278
Translation	MASVPVYCLCRLPYDVTRFMIECDMCQDWFHGSCVGVEEEKAADIDLYHCPNCEVLHGPSIMKKRRGSSKGHDNHKGKPLKTGSSMFIRELRGRTFDSSDEVILKPTGSQLTVEFLEENSFSVPILVLKKDGLGMTLPSPSFTVRDVEHYVGSDKEIDVIDVARQADCKMKLGDFVKYYYSGKREKVLNVISLEFSDTRLSNLVETPKIVRKLSWVENLWPEECVFERPNVQKYCLMSVRDSYTDFHIDFGGTSVWYHVLKGEKIFYLIRPTNANLTLFECWSSSSNQNEMFFGDQVEKCYKCSVKQGQTLFIPTGWIHAVLTPVDCLAFGGNFLHSLNIEMQLKAYEIEKRLSTADLFKFPNFETICWYVGKHILDIFRGLRENRRHPASYLVHGGKALNLAFRAWTRKEALPDHEDEIPETVRTVQLIKDLAREIRLVEDIFQQNVGKTSNIFGLQRIFPAGSIPLTRPAHSTSVSMSKLSLPSKNGSKKKGLKPKDIFKKAERKGKQSSALGPAGQLSYNLMDPYSHQALKTGPSQKAKLNIGGACLNDSDDDSADMDVDGSENPLALLMANGSTKRMKSVSKSRRAKIAKKVDSARLVAEQVMGDEFDLDSDDELQIDERLGKEKANLLMRSKFPRKLPRAKPCSDPNRIREPGEVEFDIEEDYTTDEDMVEGVESKLGNGSGAGGILDLLKASRQVGGPDYAALTEAPASPSTQEAIQGMLCMANLQSSSSSPATSSLQAWWTGGQDRSSGSSSSGLGTVSNSPISQRTPGKRPIKRPAYWKNESEEEENASMDEQDSLGACFKDAEYIYPSLESDDDDPALKSRPKKKKNSDDAPWSPKARVTPTLPKQDRPVREGTRVASIETGLAAAAAKLAQQELQKAQKKKYIKKKPLLKEVEQPRPQDSNTTMTMPAPTLATTPQPVTSSSPQPPPEPKQEALSGSLADHEYTARPNAFGMAQANRSTTPMAPGVFLTQRRPSVGSQSSQAGQGKRPKKGLATAKQRLGRILKIHRNGKLLL

Target ORF information:

RefSeq Version	XM_008773119.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus PHD finger protein 8 (Phf8), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008773119.1

ORF Insert Sequence:

ATGGCCTCGG TGCCTGTGTA TTGCCTCTGT CGACTGCCTT ACGATGTGAC CCGCTTCATG 
ATCGAGTGTG ACATGTGCCA GGACTGGTTT CACGGCAGTT GTGTTGGTGT TGAGGAGGAG 
AAGGCTGCTG ATATTGATCT GTACCATTGC CCCAACTGTG AAGTCTTACA CGGACCCTCC 
ATTATGAAAA AACGTCGTGG ATCTTCCAAA GGACATGATA ATCACAAGGG TAAACCATTG 
AAGACTGGGA GCTCCATGTT TATCCGAGAA CTACGGGGTA GGACTTTTGA CAGCTCAGAC 
GAAGTGATTC TGAAACCCAC TGGAAGTCAG CTGACAGTGG AATTCTTGGA AGAGAATAGC 
TTCAGCGTGC CTATCCTTGT CTTGAAGAAG GATGGCTTGG GGATGACATT ACCCTCTCCA 
TCATTCACTG TGAGGGATGT GGAGCACTAT GTTGGTTCTG ACAAAGAGAT TGATGTGATT 
GATGTGGCCC GCCAGGCTGA CTGCAAAATG AAACTTGGTG ATTTTGTCAA ATACTATTAC 
AGTGGGAAGA GGGAAAAAGT CCTCAATGTC ATTAGTTTGG AATTCTCTGA TACCAGACTT 
TCAAACCTTG TGGAAACACC CAAGATTGTT CGAAAGCTGT CGTGGGTGGA GAACTTGTGG 
CCGGAGGAAT GTGTCTTTGA GAGGCCCAAT GTGCAGAAGT ACTGCCTCAT GAGTGTGCGG 
GATAGCTATA CAGATTTTCA CATTGACTTT GGTGGGACCT CAGTTTGGTA CCATGTGCTT 
AAGGGTGAGA AGATCTTCTA CCTGATCCGC CCAACAAATG CTAATCTGAC TCTCTTTGAG 
TGCTGGAGCA GCTCCTCTAA TCAGAATGAG ATGTTCTTTG GTGACCAGGT GGAAAAATGC 
TACAAGTGTT CTGTGAAGCA AGGACAAACA CTGTTTATTC CTACAGGGTG GATACATGCT 
GTGCTAACAC CAGTGGACTG CTTAGCCTTT GGAGGGAACT TTTTACATAG CCTTAACATT 
GAAATGCAAC TCAAGGCCTA TGAAATTGAG AAGCGGCTGA GCACAGCAGA CCTTTTCAAG 
TTTCCCAACT TCGAGACCAT CTGTTGGTAT GTGGGAAAAC ATATTCTGGA CATCTTCCGA 
GGCTTACGAG AGAACAGGAG ACATCCTGCC TCCTACCTGG TCCATGGTGG TAAAGCTCTG 
AACTTGGCCT TTAGAGCCTG GACAAGGAAA GAAGCTCTGC CAGACCATGA GGATGAGATC 
CCAGAGACAG TGCGGACTGT ACAGCTCATT AAAGATCTGG CTAGGGAGAT CCGCCTGGTC 
GAAGACATCT TCCAACAGAA CGTTGGGAAG ACGAGCAATA TCTTTGGGCT GCAGAGGATC 
TTCCCAGCTG GTTCCATCCC CTTAACCAGG CCAGCCCATT CCACTTCAGT ATCCATGTCC 
AAGCTGTCAC TGCCCTCCAA AAATGGTTCA AAGAAGAAAG GCCTGAAGCC CAAGGATATC 
TTCAAGAAGG CAGAACGAAA GGGCAAGCAG AGTTCAGCCT TGGGGCCTGC TGGCCAGTTG 
AGCTATAATC TCATGGACCC ATACAGTCAT CAGGCACTGA AGACAGGCCC TTCCCAGAAA 
GCAAAGCTCA ACATTGGTGG TGCCTGCTTG AATGACTCAG ATGATGACTC AGCAGACATG 
GACGTTGATG GCAGTGAGAA CCCCCTGGCC CTGTTGATGG CTAATGGCAG TACAAAGAGG 
ATGAAGAGTG TATCCAAGTC TCGAAGAGCC AAAATTGCGA AGAAGGTAGA CAGTGCAAGA 
CTGGTAGCAG AACAGGTCAT GGGGGATGAA TTTGACTTGG ACTCAGATGA TGAGCTGCAG 
ATTGATGAGA GACTGGGAAA GGAAAAAGCG AACCTGTTAA TGAGATCAAA ATTTCCCCGG 
AAATTGCCCC GTGCAAAACC TTGCTCTGAC CCCAACCGAA TTCGTGAACC TGGAGAAGTT 
GAGTTTGACA TTGAGGAGGA CTATACAACA GATGAGGACA TGGTGGAAGG GGTTGAAAGC 
AAGCTTGGTA ATGGGAGTGG AGCTGGTGGA ATTCTTGATC TACTTAAGGC CAGCAGGCAG 
GTCGGGGGAC CTGACTATGC TGCCCTCACT GAGGCCCCAG CCTCCCCCAG CACACAAGAG 
GCCATCCAGG GCATGTTATG TATGGCCAAC CTGCAGTCCT CATCATCCTC ACCAGCTACC 
TCCAGTCTGC AGGCTTGGTG GACCGGAGGG CAAGACCGAA GCAGTGGGAG CTCCAGCAGT 
GGGCTGGGCA CTGTGTCTAA TAGTCCTATT TCCCAGCGCA CCCCAGGGAA GCGGCCCATC 
AAGAGGCCAG CATACTGGAA AAACGAGAGT GAGGAGGAAG AGAATGCCAG TATGGATGAA 
CAAGACAGCT TGGGAGCATG CTTCAAGGAT GCTGAGTATA TCTATCCATC CCTGGAGTCT 
GATGATGATG ACCCTGCTTT GAAATCTCGA CCCAAGAAAA AGAAGAATTC AGATGATGCT 
CCATGGAGTC CTAAAGCCCG TGTGACTCCA ACTCTACCCA AGCAGGACCG TCCTGTTCGT 
GAAGGAACCA GAGTTGCCTC CATTGAGACA GGCTTGGCTG CAGCAGCTGC AAAGCTTGCC 
CAGCAGGAGC TGCAAAAGGC CCAAAAGAAG AAATATATCA AGAAGAAGCC TTTGCTGAAG 
GAGGTAGAAC AGCCTCGCCC TCAAGATTCC AATACCACCA TGACAATGCC TGCACCAACA 
CTGGCTACTA CACCCCAGCC TGTCACCTCC TCCTCACCCC AGCCACCTCC TGAGCCTAAA 
CAAGAGGCTC TGTCAGGAAG TCTTGCTGAC CATGAGTACA CTGCTCGTCC CAATGCCTTT 
GGCATGGCTC AGGCAAATCG CAGCACCACA CCTATGGCCC CTGGAGTCTT CCTCACCCAG 
CGGCGCCCTT CAGTTGGTTC CCAGAGCAGT CAGGCAGGAC AAGGAAAGCG TCCGAAAAAG 
GGCCTGGCCA CAGCAAAGCA AAGACTCGGC CGCATCCTGA AAATCCACAG AAATGGCAAG 
TTACTTCTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Phf8 ( XM_006256792.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006256854.1 Rattus norvegicus PHD finger protein 8 (Phf8), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa06988
Clone ID Related Accession (Same CDS sequence)	XM_017602072.1 , XM_008773119.1 , NM_001108253.1 , XM_006256792.2
Accession Version	XM_006256792.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3072bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	histone lysine demethylase PHF8 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005120.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 7, 2014 this sequence version replaced XM_006256792.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCTCGG TGCCTGTGTA TTGCCTCTGT CGACTGCCTT ACGATGTGAC CCGCTTCATG 
ATCGAGTGTG ACATGTGCCA GGACTGGTTT CACGGCAGTT GTGTTGGTGT TGAGGAGGAG 
AAGGCTGCTG ATATTGATCT GTACCATTGC CCCAACTGTG AAGTCTTACA CGGACCCTCC 
ATTATGAAAA AACGTCGTGG ATCTTCCAAA GGACATGATA ATCACAAGGG TAAACCATTG 
AAGACTGGGA GCTCCATGTT TATCCGAGAA CTACGGGGTA GGACTTTTGA CAGCTCAGAC 
GAAGTGATTC TGAAACCCAC TGGAAGTCAG CTGACAGTGG AATTCTTGGA AGAGAATAGC 
TTCAGCGTGC CTATCCTTGT CTTGAAGAAG GATGGCTTGG GGATGACATT ACCCTCTCCA 
TCATTCACTG TGAGGGATGT GGAGCACTAT GTTGGTTCTG ACAAAGAGAT TGATGTGATT 
GATGTGGCCC GCCAGGCTGA CTGCAAAATG AAACTTGGTG ATTTTGTCAA ATACTATTAC 
AGTGGGAAGA GGGAAAAAGT CCTCAATGTC ATTAGTTTGG AATTCTCTGA TACCAGACTT 
TCAAACCTTG TGGAAACACC CAAGATTGTT CGAAAGCTGT CGTGGGTGGA GAACTTGTGG 
CCGGAGGAAT GTGTCTTTGA GAGGCCCAAT GTGCAGAAGT ACTGCCTCAT GAGTGTGCGG 
GATAGCTATA CAGATTTTCA CATTGACTTT GGTGGGACCT CAGTTTGGTA CCATGTGCTT 
AAGGGTGAGA AGATCTTCTA CCTGATCCGC CCAACAAATG CTAATCTGAC TCTCTTTGAG 
TGCTGGAGCA GCTCCTCTAA TCAGAATGAG ATGTTCTTTG GTGACCAGGT GGAAAAATGC 
TACAAGTGTT CTGTGAAGCA AGGACAAACA CTGTTTATTC CTACAGGGTG GATACATGCT 
GTGCTAACAC CAGTGGACTG CTTAGCCTTT GGAGGGAACT TTTTACATAG CCTTAACATT 
GAAATGCAAC TCAAGGCCTA TGAAATTGAG AAGCGGCTGA GCACAGCAGA CCTTTTCAAG 
TTTCCCAACT TCGAGACCAT CTGTTGGTAT GTGGGAAAAC ATATTCTGGA CATCTTCCGA 
GGCTTACGAG AGAACAGGAG ACATCCTGCC TCCTACCTGG TCCATGGTGG TAAAGCTCTG 
AACTTGGCCT TTAGAGCCTG GACAAGGAAA GAAGCTCTGC CAGACCATGA GGATGAGATC 
CCAGAGACAG TGCGGACTGT ACAGCTCATT AAAGATCTGG CTAGGGAGAT CCGCCTGGTC 
GAAGACATCT TCCAACAGAA CGTTGGGAAG ACGAGCAATA TCTTTGGGCT GCAGAGGATC 
TTCCCAGCTG GTTCCATCCC CTTAACCAGG CCAGCCCATT CCACTTCAGT ATCCATGTCC 
AAGCTGTCAC TGCCCTCCAA AAATGGTTCA AAGAAGAAAG GCCTGAAGCC CAAGGATATC 
TTCAAGAAGG CAGAACGAAA GGGCAAGCAG AGTTCAGCCT TGGGGCCTGC TGGCCAGTTG 
AGCTATAATC TCATGGACCC ATACAGTCAT CAGGCACTGA AGACAGGCCC TTCCCAGAAA 
GCAAAGCTCA ACATTGGTGG TGCCTGCTTG AATGACTCAG ATGATGACTC AGCAGACATG 
GACGTTGATG GCAGTGAGAA CCCCCTGGCC CTGTTGATGG CTAATGGCAG TACAAAGAGG 
ATGAAGAGTG TATCCAAGTC TCGAAGAGCC AAAATTGCGA AGAAGGTAGA CAGTGCAAGA 
CTGGTAGCAG AACAGGTCAT GGGGGATGAA TTTGACTTGG ACTCAGATGA TGAGCTGCAG 
ATTGATGAGA GACTGGGAAA GGAAAAAGCG AACCTGTTAA TGAGATCAAA ATTTCCCCGG 
AAATTGCCCC GTGCAAAACC TTGCTCTGAC CCCAACCGAA TTCGTGAACC TGGAGAAGTT 
GAGTTTGACA TTGAGGAGGA CTATACAACA GATGAGGACA TGGTGGAAGG GGTTGAAAGC 
AAGCTTGGTA ATGGGAGTGG AGCTGGTGGA ATTCTTGATC TACTTAAGGC CAGCAGGCAG 
GTCGGGGGAC CTGACTATGC TGCCCTCACT GAGGCCCCAG CCTCCCCCAG CACACAAGAG 
GCCATCCAGG GCATGTTATG TATGGCCAAC CTGCAGTCCT CATCATCCTC ACCAGCTACC 
TCCAGTCTGC AGGCTTGGTG GACCGGAGGG CAAGACCGAA GCAGTGGGAG CTCCAGCAGT 
GGGCTGGGCA CTGTGTCTAA TAGTCCTATT TCCCAGCGCA CCCCAGGGAA GCGGCCCATC 
AAGAGGCCAG CATACTGGAA AAACGAGAGT GAGGAGGAAG AGAATGCCAG TATGGATGAA 
CAAGACAGCT TGGGAGCATG CTTCAAGGAT GCTGAGTATA TCTATCCATC CCTGGAGTCT 
GATGATGATG ACCCTGCTTT GAAATCTCGA CCCAAGAAAA AGAAGAATTC AGATGATGCT 
CCATGGAGTC CTAAAGCCCG TGTGACTCCA ACTCTACCCA AGCAGGACCG TCCTGTTCGT 
GAAGGAACCA GAGTTGCCTC CATTGAGACA GGCTTGGCTG CAGCAGCTGC AAAGCTTGCC 
CAGCAGGAGC TGCAAAAGGC CCAAAAGAAG AAATATATCA AGAAGAAGCC TTTGCTGAAG 
GAGGTAGAAC AGCCTCGCCC TCAAGATTCC AATACCACCA TGACAATGCC TGCACCAACA 
CTGGCTACTA CACCCCAGCC TGTCACCTCC TCCTCACCCC AGCCACCTCC TGAGCCTAAA 
CAAGAGGCTC TGTCAGGAAG TCTTGCTGAC CATGAGTACA CTGCTCGTCC CAATGCCTTT 
GGCATGGCTC AGGCAAATCG CAGCACCACA CCTATGGCCC CTGGAGTCTT CCTCACCCAG 
CGGCGCCCTT CAGTTGGTTC CCAGAGCAGT CAGGCAGGAC AAGGAAAGCG TCCGAAAAAG 
GGCCTGGCCA CAGCAAAGCA AAGACTCGGC CGCATCCTGA AAATCCACAG AAATGGCAAG 
TTACTTCTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006256854.1
CDS	1069..4140
Translation	MASVPVYCLCRLPYDVTRFMIECDMCQDWFHGSCVGVEEEKAADIDLYHCPNCEVLHGPSIMKKRRGSSKGHDNHKGKPLKTGSSMFIRELRGRTFDSSDEVILKPTGSQLTVEFLEENSFSVPILVLKKDGLGMTLPSPSFTVRDVEHYVGSDKEIDVIDVARQADCKMKLGDFVKYYYSGKREKVLNVISLEFSDTRLSNLVETPKIVRKLSWVENLWPEECVFERPNVQKYCLMSVRDSYTDFHIDFGGTSVWYHVLKGEKIFYLIRPTNANLTLFECWSSSSNQNEMFFGDQVEKCYKCSVKQGQTLFIPTGWIHAVLTPVDCLAFGGNFLHSLNIEMQLKAYEIEKRLSTADLFKFPNFETICWYVGKHILDIFRGLRENRRHPASYLVHGGKALNLAFRAWTRKEALPDHEDEIPETVRTVQLIKDLAREIRLVEDIFQQNVGKTSNIFGLQRIFPAGSIPLTRPAHSTSVSMSKLSLPSKNGSKKKGLKPKDIFKKAERKGKQSSALGPAGQLSYNLMDPYSHQALKTGPSQKAKLNIGGACLNDSDDDSADMDVDGSENPLALLMANGSTKRMKSVSKSRRAKIAKKVDSARLVAEQVMGDEFDLDSDDELQIDERLGKEKANLLMRSKFPRKLPRAKPCSDPNRIREPGEVEFDIEEDYTTDEDMVEGVESKLGNGSGAGGILDLLKASRQVGGPDYAALTEAPASPSTQEAIQGMLCMANLQSSSSSPATSSLQAWWTGGQDRSSGSSSSGLGTVSNSPISQRTPGKRPIKRPAYWKNESEEEENASMDEQDSLGACFKDAEYIYPSLESDDDDPALKSRPKKKKNSDDAPWSPKARVTPTLPKQDRPVREGTRVASIETGLAAAAAKLAQQELQKAQKKKYIKKKPLLKEVEQPRPQDSNTTMTMPAPTLATTPQPVTSSSPQPPPEPKQEALSGSLADHEYTARPNAFGMAQANRSTTPMAPGVFLTQRRPSVGSQSSQAGQGKRPKKGLATAKQRLGRILKIHRNGKLLL

Target ORF information:

RefSeq Version	XM_006256792.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus PHD finger protein 8 (Phf8), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006256792.2

ORF Insert Sequence:

ATGGCCTCGG TGCCTGTGTA TTGCCTCTGT CGACTGCCTT ACGATGTGAC CCGCTTCATG 
ATCGAGTGTG ACATGTGCCA GGACTGGTTT CACGGCAGTT GTGTTGGTGT TGAGGAGGAG 
AAGGCTGCTG ATATTGATCT GTACCATTGC CCCAACTGTG AAGTCTTACA CGGACCCTCC 
ATTATGAAAA AACGTCGTGG ATCTTCCAAA GGACATGATA ATCACAAGGG TAAACCATTG 
AAGACTGGGA GCTCCATGTT TATCCGAGAA CTACGGGGTA GGACTTTTGA CAGCTCAGAC 
GAAGTGATTC TGAAACCCAC TGGAAGTCAG CTGACAGTGG AATTCTTGGA AGAGAATAGC 
TTCAGCGTGC CTATCCTTGT CTTGAAGAAG GATGGCTTGG GGATGACATT ACCCTCTCCA 
TCATTCACTG TGAGGGATGT GGAGCACTAT GTTGGTTCTG ACAAAGAGAT TGATGTGATT 
GATGTGGCCC GCCAGGCTGA CTGCAAAATG AAACTTGGTG ATTTTGTCAA ATACTATTAC 
AGTGGGAAGA GGGAAAAAGT CCTCAATGTC ATTAGTTTGG AATTCTCTGA TACCAGACTT 
TCAAACCTTG TGGAAACACC CAAGATTGTT CGAAAGCTGT CGTGGGTGGA GAACTTGTGG 
CCGGAGGAAT GTGTCTTTGA GAGGCCCAAT GTGCAGAAGT ACTGCCTCAT GAGTGTGCGG 
GATAGCTATA CAGATTTTCA CATTGACTTT GGTGGGACCT CAGTTTGGTA CCATGTGCTT 
AAGGGTGAGA AGATCTTCTA CCTGATCCGC CCAACAAATG CTAATCTGAC TCTCTTTGAG 
TGCTGGAGCA GCTCCTCTAA TCAGAATGAG ATGTTCTTTG GTGACCAGGT GGAAAAATGC 
TACAAGTGTT CTGTGAAGCA AGGACAAACA CTGTTTATTC CTACAGGGTG GATACATGCT 
GTGCTAACAC CAGTGGACTG CTTAGCCTTT GGAGGGAACT TTTTACATAG CCTTAACATT 
GAAATGCAAC TCAAGGCCTA TGAAATTGAG AAGCGGCTGA GCACAGCAGA CCTTTTCAAG 
TTTCCCAACT TCGAGACCAT CTGTTGGTAT GTGGGAAAAC ATATTCTGGA CATCTTCCGA 
GGCTTACGAG AGAACAGGAG ACATCCTGCC TCCTACCTGG TCCATGGTGG TAAAGCTCTG 
AACTTGGCCT TTAGAGCCTG GACAAGGAAA GAAGCTCTGC CAGACCATGA GGATGAGATC 
CCAGAGACAG TGCGGACTGT ACAGCTCATT AAAGATCTGG CTAGGGAGAT CCGCCTGGTC 
GAAGACATCT TCCAACAGAA CGTTGGGAAG ACGAGCAATA TCTTTGGGCT GCAGAGGATC 
TTCCCAGCTG GTTCCATCCC CTTAACCAGG CCAGCCCATT CCACTTCAGT ATCCATGTCC 
AAGCTGTCAC TGCCCTCCAA AAATGGTTCA AAGAAGAAAG GCCTGAAGCC CAAGGATATC 
TTCAAGAAGG CAGAACGAAA GGGCAAGCAG AGTTCAGCCT TGGGGCCTGC TGGCCAGTTG 
AGCTATAATC TCATGGACCC ATACAGTCAT CAGGCACTGA AGACAGGCCC TTCCCAGAAA 
GCAAAGCTCA ACATTGGTGG TGCCTGCTTG AATGACTCAG ATGATGACTC AGCAGACATG 
GACGTTGATG GCAGTGAGAA CCCCCTGGCC CTGTTGATGG CTAATGGCAG TACAAAGAGG 
ATGAAGAGTG TATCCAAGTC TCGAAGAGCC AAAATTGCGA AGAAGGTAGA CAGTGCAAGA 
CTGGTAGCAG AACAGGTCAT GGGGGATGAA TTTGACTTGG ACTCAGATGA TGAGCTGCAG 
ATTGATGAGA GACTGGGAAA GGAAAAAGCG AACCTGTTAA TGAGATCAAA ATTTCCCCGG 
AAATTGCCCC GTGCAAAACC TTGCTCTGAC CCCAACCGAA TTCGTGAACC TGGAGAAGTT 
GAGTTTGACA TTGAGGAGGA CTATACAACA GATGAGGACA TGGTGGAAGG GGTTGAAAGC 
AAGCTTGGTA ATGGGAGTGG AGCTGGTGGA ATTCTTGATC TACTTAAGGC CAGCAGGCAG 
GTCGGGGGAC CTGACTATGC TGCCCTCACT GAGGCCCCAG CCTCCCCCAG CACACAAGAG 
GCCATCCAGG GCATGTTATG TATGGCCAAC CTGCAGTCCT CATCATCCTC ACCAGCTACC 
TCCAGTCTGC AGGCTTGGTG GACCGGAGGG CAAGACCGAA GCAGTGGGAG CTCCAGCAGT 
GGGCTGGGCA CTGTGTCTAA TAGTCCTATT TCCCAGCGCA CCCCAGGGAA GCGGCCCATC 
AAGAGGCCAG CATACTGGAA AAACGAGAGT GAGGAGGAAG AGAATGCCAG TATGGATGAA 
CAAGACAGCT TGGGAGCATG CTTCAAGGAT GCTGAGTATA TCTATCCATC CCTGGAGTCT 
GATGATGATG ACCCTGCTTT GAAATCTCGA CCCAAGAAAA AGAAGAATTC AGATGATGCT 
CCATGGAGTC CTAAAGCCCG TGTGACTCCA ACTCTACCCA AGCAGGACCG TCCTGTTCGT 
GAAGGAACCA GAGTTGCCTC CATTGAGACA GGCTTGGCTG CAGCAGCTGC AAAGCTTGCC 
CAGCAGGAGC TGCAAAAGGC CCAAAAGAAG AAATATATCA AGAAGAAGCC TTTGCTGAAG 
GAGGTAGAAC AGCCTCGCCC TCAAGATTCC AATACCACCA TGACAATGCC TGCACCAACA 
CTGGCTACTA CACCCCAGCC TGTCACCTCC TCCTCACCCC AGCCACCTCC TGAGCCTAAA 
CAAGAGGCTC TGTCAGGAAG TCTTGCTGAC CATGAGTACA CTGCTCGTCC CAATGCCTTT 
GGCATGGCTC AGGCAAATCG CAGCACCACA CCTATGGCCC CTGGAGTCTT CCTCACCCAG 
CGGCGCCCTT CAGTTGGTTC CCAGAGCAGT CAGGCAGGAC AAGGAAAGCG TCCGAAAAAG 
GGCCTGGCCA CAGCAAAGCA AAGACTCGGC CGCATCCTGA AAATCCACAG AAATGGCAAG 
TTACTTCTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Histone lysine methylation dynamics: establishment, regulation, and biological impact.
Molecular cell48(4)491-507(2012 Nov)
Black JC,Van Rechem C,Whetstine JR

Systematic knockdown of epigenetic enzymes identifies a novel histone demethylase PHF8 overexpressed in prostate cancer with an impact on cell proliferation, migration and invasion.
Oncogene31(29)3444-56(2012 Jul)
Bj?rkman M,?stling P,H?rm? V,Virtanen J,Mpindi JP,Rantala J,Mirtti T,Vesterinen T,Lundin M,Sankila A,Rannikko A,Kaivanto E,Kohonen P,Kallioniemi O,Nees M

Nucleosome-interacting proteins regulated by DNA and histone methylation.
Cell143(3)470-84(2010 Oct)
Bartke T,Vermeulen M,Xhemalce B,Robson SC,Mann M,Kouzarides T

PHF8 mediates histone H4 lysine 20 demethylation events involved in cell cycle progression.
Nature466(7305)508-12(2010 Jul)
Liu W,Tanasa B,Tyurina OV,Zhou TY,Gassmann R,Liu WT,Ohgi KA,Benner C,Garcia-Bassets I,Aggarwal AK,Desai A,Dorrestein PC,Glass CK,Rosenfeld MG

Histone H4K20/H3K9 demethylase PHF8 regulates zebrafish brain and craniofacial development.
Nature466(7305)503-7(2010 Jul)
Qi HH,Sarkissian M,Hu GQ,Wang Z,Bhattacharjee A,Gordon DB,Gonzales M,Lan F,Ongusaha PP,Huarte M,Yaghi NK,Lim H,Garcia BA,Brizuela L,Zhao K,Roberts TM,Shi Y

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Phf8 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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