Sned1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Sned1
Entrez Gene ID	316638
Full Name	sushi, nidogen and EGF-like domains 1
Synonyms	IRE-BP1
General protein information	Preferred Names sushi, nidogen and EGF-like domains 1 Names sushi, nidogen and EGF-like domain-containing protein 1 insulin-responsive sequence DNA-binding protein 1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 9 Map Location: 9q36

mRNA and Protein(s)

mRNA	Protein	Name
XM_008767374.2	XP_008765596.1	sushi, nidogen and EGF-like domain-containing protein 1 isoform X2
XM_006245526.3	XP_006245588.1	sushi, nidogen and EGF-like domain-containing protein 1 isoform X3
XM_017596495.1	XP_017451984.1	sushi, nidogen and EGF-like domain-containing protein 1 isoform X4
XM_008767373.2	XP_008765595.1	sushi, nidogen and EGF-like domain-containing protein 1 isoform X1
NM_001167842.1	NP_001161314.1	sushi, nidogen and EGF-like domain-containing protein 1 precursor

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	SNED1	XP_005636015.1
Gallus gallus (chicken)	SNED1	XP_003641796.1
Xenopus tropicalis (tropical clawed frog)	sned1	XP_002933383.2
Pan troglodytes (chimpanzee)	SNED1	XP_003309615.1
Mus musculus (house mouse)	Sned1	NP_766051.4
Danio rerio (zebrafish)	sned1	XP_001341690.4
Rattus norvegicus (Norway rat)	Sned1	NP_001161314.1
Homo sapiens (human)	SNED1	NP_001073906.1

Gene Ontology
Bibliography

Related articles in PubMed

Transgenic expression of insulin-response element binding protein-1 in beta-cells reproduces type 2 diabetes.
Villafuerte BC, Barati MT, Song Y, Moore JP, Epstein PN, Portillo J
Endocrinology150(6)2611-7(2009 Jun)

Regulation of insulin-response element binding protein-1 in obesity and diabetes: potential role in impaired insulin-induced gene transcription.
Chahal J, Chen CC, Rane MJ, Moore JP, Barati MT, Song Y, Villafuerte BC
Endocrinology149(10)4829-36(2008 Oct)

An insulin-response element-binding protein that ameliorates hyperglycemia in diabetes.
Villafuerte BC, Kaytor EN
The Journal of biological chemistry280(20)20010-20(2005 May)

Insulin-response element-binding protein 1: a novel Akt substrate involved in transcriptional action of insulin.
Villafuerte BC, Phillips LS, Rane MJ, Zhao W
The Journal of biological chemistry279(35)36650-9(2004 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Increased gene transcription mediated through IRE-BP1 may contribute to beta-cell dysfunction in insulin resistance, and allow for the hypothesis that IRE-BP1 plays a role in the pathophysiology of type 2 diabetes.
Title: Transgenic expression of insulin-response element binding protein-1 in beta-cells reproduces type 2 diabetes.

study supports the notion that IRE-BP1 may be relevant to the action of insulin in vivo and may play a role in the development of insulin resistance and diabetes
Title: Regulation of insulin-response element binding protein-1 in obesity and diabetes: potential role in impaired insulin-induced gene transcription.

stable constitutive expression of IRE-BP1 enhanced glucose transporter expression, glucose uptake, and glycogen accumulation
Title: An insulin-response element-binding protein that ameliorates hyperglycemia in diabetes.

identified a novel transcription factor,insulin-response element-binding protein 1, that binds and transactivates the insulin-response elements of the insulin-like growth factor-binding protein-3 and other insulin responsive genes
Title: Insulin-response element-binding protein 1: a novel Akt substrate involved in transcriptional action of insulin.

The following Sned1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Sned1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa05024	NM_001167842.1 Latest version!	Rattus norvegicus sushi, nidogen and EGF-like domains 1 (Sned1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$727.30 $1039.00
ORa32574	XM_006245526.3 Latest version!	Rattus norvegicus sushi, nidogen and EGF-like domains 1 (Sned1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$727.30 $1039.00
ORa47568	XM_008767373.2 Latest version!	Rattus norvegicus sushi, nidogen and EGF-like domains 1 (Sned1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$811.30 $1159.00
ORa47569	XM_008767374.2 Latest version!	Rattus norvegicus sushi, nidogen and EGF-like domains 1 (Sned1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$727.30 $1039.00
ORa62788	XM_017596495.1 Latest version!	Rattus norvegicus sushi, nidogen and EGF-like domains 1 (Sned1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$727.30 $1039.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Sned1 ( NM_001167842.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001161314.1 Rattus norvegicus sushi, nidogen and EGF-like domains 1 (Sned1), mRNA.

Price & Availability↑

CloneID	ORa05024
Clone ID Related Accession (Same CDS sequence)	NM_001167842.1
Accession Version	NM_001167842.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4194bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-17
Organism	Rattus norvegicus(Norway rat)
Product	sushi, nidogen and EGF-like domain-containing protein 1 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CH473997.2. On or before Nov 19, 2009 this sequence version replaced XM_237415.4, XM_001068874.1. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGCGCCGTG GCGCCGCCTG GGCGCTGCTG CTGGCCGCAG CCCTGGGGCT CGGGGCGCGC 
GGGGTGCGCG CTGCGGTGGC CCTCGCCGAC TTCTACCCGT TCGGCACGAA GCGTGGCGAC 
GCCGTCACCC CGAAGCAGGA CGACGGCGGC TCAGGGCTGC AGCCCCTCTC GGTGCCCTTC 
CCGTTCTTCG GCGCCGAGCA CTCAGGACTC TACGTGAACA ACAACGGGAT CATCTCCTTC 
CTGAAGGAAG TTTCTCAGTT CACCCCTGTG GCCTTCCCCA TCGCCAAAGA CCGCTGTGTG 
GTAGCGGCCT TCTGGGCAGA CGTGGATAAC CGACGCGCAG GCGACGTCTA CTACCGGGAG 
GCCACCGACG CAGCCATGCT GAACAGAGCC ACGGAGGACA TCAGGCGGTA CTTTCCTGAG 
CTCCCGGACT TCTCTGCTAC CTGGGTTTTT GTTGCCACCT GGTACCGTGT GACCTTCTTC 
GGAGGCAGCA GCTCTTCCCC CGTCAACACA TTCCAAACGG TGCTCATCAC TGACGGCCGG 
TTCTCCTTCA CCATCTTCAA CTATGAGTCC ATCCTGTGGA CTACGGGCAC ACACGCCAGC 
AGTGGTGGTG ATGCTGATGG CTTGGGAGGC ATTGCAGCCC AGGCAGGTTT CAACGCAGGT 
GATGGGCACC GCTACTTCAA CATCCCTGGG TCGCGCACAG CAGACATGGC TGAGGTGGAG 
ACCACCACCA ACGTGGGCGT GCCCGGGCGC TGGGCGTTTA GAATCGATGA TGCCCAGGTG 
CGCGTGGGGG GCTGCGGCCA TACAACCTCT GTGTGCCTAG TCCTGCGTCC GTGCCTCAAT 
GGTGGCAAGT GCATCGATGA CTGTGTCACG GGCAATCCCT CCTACACCTG TTCCTGTCTC 
GCTGGCTTCA CGGGGCGTAG ATGCCACCTG GATGTGAACG AGTGTGCTTC CCACCCATGT 
CAGAACGGTG GGACCTGCAC CCACGGTGTC AACAGCTTCA GTTGCCAGTG CCCAGCCGGC 
TTCCAGGGAC CCACCTGTGA ATCAGCCCAG TCTCCGTGTG ACAACAAAGT GTGTCAAAAT 
GGTGGCCAGT GCCAGGCAGA GAGCAGCTCT GCAGTATGTG TGTGTCAAGC TGGATACACT 
GGGGCCACCT GTGAGACCGA TGTGGATGAA TGCAGCTCTG ACCCATGCCT AAATGGTGGA 
TCGTGTGTTG ACCTGGTTGG AAACTACAGC TGTATTTGTG TGGAGCCCTT TGAAGGACCT 
CAGTGCGAGA CAGGAAGCTA CGTGGTGCCT TCGCCCTGCC TCTCCAACCC CTGCCTGAAC 
GGGGGCACCT GTGTGGATGC TGACCAGGGA TACGTGTGCG AATGCCCTGA AGGTTTCATG 
GGCTTGGACT GCAGAGAGAG AATTCTCAAT GACTGTGATT GCCGGAATGG AGGCAGATGC 
CTGGGTGCCA ACACCACCAT CTGCCAGTGT CCTCCAGGCT TCTTTGGGCT CCTCTGTGAA 
TTTGAAGTCA CAGCCACGCC CTGCAACATG AACACTCAGT GTCCAGATGG AGGCTACTGC 
ATGGAGTATG GCGGAAGCTA CCTATGTGTC TGCCACACGG ACCACAACAT CAGCCATTCT 
CTGCCCTCGC CCTGCGACTC AGACCCATGC TTTAATGGAG GTTCCTGTGA CGCCCACGAG 
GACTCCTACA CGTGCGAGTG CCCTCGTGGA TTCCATGGCA GGCACTGCGA GAAAGCCCGG 
CCACACCTGT GCAGCTCAGG GCCCTGCCGG AATGGGGGCA CATGCAAGGA GACTGGTGAC 
GAGTACCGCT GCACCTGCCC TTACCGGTTC ACTGGGAGAC ACTGTGAGAT TGGAAAGCCA 
GACTCCTGTG CCTCTGGCCC CTGTCACAAC GGTGGCACTT GTTTCCACTA CATTGGCAAA 
TACAAGTGTG ACTGCCCTCC AGGCTTCTCT GGTCGGCACT GTGAGATAGC CCCCTCCCCC 
TGCTTCCGGA GCCCATGTAT GAATGGGGGT ATCTGCGAGG ATCTAGGAAC AGATTTCTCC 
TGCCACTGCC AACCAGGATA TACAGGACAC CGGTGTCAGG CAGAGGTGGA CTGCGGTCAG 
CCTGAGGAGG TAAAACATGC TACCATGCGT CTCAATGGAA CTCGCATGGG CTCGGTGGCC 
CTGTACACAT GTGACCCCGG CTTCAGCCTG AGCGTCCTCA GCCATATGCG TGTCTGTCAG 
CCACAAGGTG TCTGGAGCCA GCCTCCCCAG TGCATTGAAG TAGATGAGTG CCAGTCTCAG 
CCATGCCTGC ATAAAGGCTC CTGCCAGGAC CTCATTGCTG GTTACCAGTG CCTCTGCAGC 
CCGGGGTACG AAGGAGTCCA CTGTGAGCTA GAGACAGACG AGTGCCAAGC ACAGCCCTGC 
AGAAATGGAG GCTCCTGCAG GGACCTCCCC GGGGCTTTCA TCTGCCAGTG CCCTGAAGGT 
TTTGTTGGAA CCCACTGTGA AACAGAGGTG GATGCCTGTG CCTCCAGCCC CTGCCAGCAC 
GGAGGCCGGT GTGAGGACGG TGGTGGGGCC TACCTGTGCG TGTGTCCAGA GGGCTTCTTC 
GGCTACAACT GTGAGACAGT GAGTGACCCC TGCTTCTCTA GCCCCTGTGG GGGCCGCGGC 
TACTGCTTGG CCAGCAACGG GTCCCACAGC TGTACCTGCA AAGTGGGCTA CACAGGCAAG 
GACTGCACCA AAGCCCTCAG GGTAGAAAGG GTGGAGGAGA GTGGGGTCTC CATCTCCTGG 
AGCCCACCCG AGGGCACCAC GGCCAGACAG GTGCTGGACG GCTATGCAGT CACCTATGCC 
TCCTCGGATG GATCGTCCAG GCGCACGGAC TTTGTGGACC GGAGCCGCTC CTCTCACCAG 
CTTCGGGCCC TGGCAGCCGG CCGTGCCTAC AACATCTCTG TTTTCTCAGT CAAGAGAAAC 
ACTAACAACA AAAATGACAT CAGCAGGCCT GCAGCCCTGC TCACCCGCAC CCGACCCCGC 
CCTATTGAAG ACTTCGAGGT CACCAACATT TCAGCCAATG CCATCTCAGT GCAGTGGGCT 
CTTCATAGGA TCCAGCATGC CACTGTCAGC AGGGTTCGAG TGTCTGTCCT CTACCCTGAG 
GACACTGTGG TCCAGTCCAC GGAGGTGGAC AGGAGTGTGG ACCGCCTCAC ATTTGGGGAC 
CTGCTGCCAG GGAGAAGATA CAGTGTGCGG CTAACCACCC TCAGTGGGCC TGGAGGAGCT 
GAATATCCTA CAGAGAGCCT GGCCTCAGCT CCGCTGAACG TGTGGACCCG GCCTTTGCCT 
CCAGCAAACC TGACTGCCTC TCGAGTCACA GCGACCTCTG CCCATATGGT CTGGGACCCG 
CCCACTCCAG GCATCTCACT GGAGGCTTAC GTCATCAATG TGACCACCAG TCAGAATACC 
AAGAGCCGCT ACATCCCCAA TGGGAAGCTG GTGTCCTATA CGGTGCGTGA TCTGATGCCA 
GGTCGGCGGT ACCAGCTCTC GGTCACAGCG GTGCAGAGCA CAGAGCAGGG CCAGCTGCAC 
AGTGAGCCTG CGCACCTCTA CATCATCACC TCCCCCAGGG ATGGCACCGA CAGGCGCTGG 
CACCAGGGAG GACACCACTC ACGAATGCTC AGAAATAGGC CGGCCCCTTT GCGCCTGCCA 
GAACTGCGCC TCCTCAATGA CCACGGTGCC CCTGAAACAC CAACCCAGCC ACCCAGGTTC 
TCAGAGCTTG TAGACGGAAG AGCAAGAGTG AGTGCCAGGT TTGGTGGATT GCCCAGCAGA 
GCAGTAACTG TGAGATCACA ACCCACTACT CCGGTGCCGC TCAAGAACAC AGAGGCCCCT 
GAGCAGGCCC GTCTGGCCCT TCAGCTACCC AAGAACAACA GCAAGGACAC AGAAAGTACC 
CCTGGCAGCT GTTCAGAAGA CACCTGTCAG AATGGAGGCA CCTGTGTCCC AGGTGCCAAT 
GCCCACAGCT GTGACTGCAG GCCTGGGTTC AAAGGCAGAC ACTGTGAGCT TGCCTGTGAA 
AAAGTGCCCC GCCCCTGCAC ACGGCTGTTC TCTGAGACCA AGTCATTTCC TGTCTGGGAA 
GGAGATGTCT GCCACCATGT GTATAAGAAA GTCTACAAAG TTCACCAGGA CGTGTGTTTT 
AAGGAGCGCT GCCAGAGCAC AAGCCTCAAA AAGCTCAAAC AGGAATCAAA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001161314.1
CDS	152..4345
Translation	MRRGAAWALLLAAALGLGARGVRAAVALADFYPFGTKRGDAVTPKQDDGGSGLQPLSVPFPFFGAEHSGLYVNNNGIISFLKEVSQFTPVAFPIAKDRCVVAAFWADVDNRRAGDVYYREATDAAMLNRATEDIRRYFPELPDFSATWVFVATWYRVTFFGGSSSSPVNTFQTVLITDGRFSFTIFNYESILWTTGTHASSGGDADGLGGIAAQAGFNAGDGHRYFNIPGSRTADMAEVETTTNVGVPGRWAFRIDDAQVRVGGCGHTTSVCLVLRPCLNGGKCIDDCVTGNPSYTCSCLAGFTGRRCHLDVNECASHPCQNGGTCTHGVNSFSCQCPAGFQGPTCESAQSPCDNKVCQNGGQCQAESSSAVCVCQAGYTGATCETDVDECSSDPCLNGGSCVDLVGNYSCICVEPFEGPQCETGSYVVPSPCLSNPCLNGGTCVDADQGYVCECPEGFMGLDCRERILNDCDCRNGGRCLGANTTICQCPPGFFGLLCEFEVTATPCNMNTQCPDGGYCMEYGGSYLCVCHTDHNISHSLPSPCDSDPCFNGGSCDAHEDSYTCECPRGFHGRHCEKARPHLCSSGPCRNGGTCKETGDEYRCTCPYRFTGRHCEIGKPDSCASGPCHNGGTCFHYIGKYKCDCPPGFSGRHCEIAPSPCFRSPCMNGGICEDLGTDFSCHCQPGYTGHRCQAEVDCGQPEEVKHATMRLNGTRMGSVALYTCDPGFSLSVLSHMRVCQPQGVWSQPPQCIEVDECQSQPCLHKGSCQDLIAGYQCLCSPGYEGVHCELETDECQAQPCRNGGSCRDLPGAFICQCPEGFVGTHCETEVDACASSPCQHGGRCEDGGGAYLCVCPEGFFGYNCETVSDPCFSSPCGGRGYCLASNGSHSCTCKVGYTGKDCTKALRVERVEESGVSISWSPPEGTTARQVLDGYAVTYASSDGSSRRTDFVDRSRSSHQLRALAAGRAYNISVFSVKRNTNNKNDISRPAALLTRTRPRPIEDFEVTNISANAISVQWALHRIQHATVSRVRVSVLYPEDTVVQSTEVDRSVDRLTFGDLLPGRRYSVRLTTLSGPGGAEYPTESLASAPLNVWTRPLPPANLTASRVTATSAHMVWDPPTPGISLEAYVINVTTSQNTKSRYIPNGKLVSYTVRDLMPGRRYQLSVTAVQSTEQGQLHSEPAHLYIITSPRDGTDRRWHQGGHHSRMLRNRPAPLRLPELRLLNDHGAPETPTQPPRFSELVDGRARVSARFGGLPSRAVTVRSQPTTPVPLKNTEAPEQARLALQLPKNNSKDTESTPGSCSEDTCQNGGTCVPGANAHSCDCRPGFKGRHCELACEKVPRPCTRLFSETKSFPVWEGDVCHHVYKKVYKVHQDVCFKERCQSTSLKKLKQESN

Target ORF information:

RefSeq Version	NM_001167842.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus sushi, nidogen and EGF-like domains 1 (Sned1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001167842.1

ORF Insert Sequence:

ATGCGCCGTG GCGCCGCCTG GGCGCTGCTG CTGGCCGCAG CCCTGGGGCT CGGGGCGCGC 
GGGGTGCGCG CTGCGGTGGC CCTCGCCGAC TTCTACCCGT TCGGCACGAA GCGTGGCGAC 
GCCGTCACCC CGAAGCAGGA CGACGGCGGC TCAGGGCTGC AGCCCCTCTC GGTGCCCTTC 
CCGTTCTTCG GCGCCGAGCA CTCAGGACTC TACGTGAACA ACAACGGGAT CATCTCCTTC 
CTGAAGGAAG TTTCTCAGTT CACCCCTGTG GCCTTCCCCA TCGCCAAAGA CCGCTGTGTG 
GTAGCGGCCT TCTGGGCAGA CGTGGATAAC CGACGCGCAG GCGACGTCTA CTACCGGGAG 
GCCACCGACG CAGCCATGCT GAACAGAGCC ACGGAGGACA TCAGGCGGTA CTTTCCTGAG 
CTCCCGGACT TCTCTGCTAC CTGGGTTTTT GTTGCCACCT GGTACCGTGT GACCTTCTTC 
GGAGGCAGCA GCTCTTCCCC CGTCAACACA TTCCAAACGG TGCTCATCAC TGACGGCCGG 
TTCTCCTTCA CCATCTTCAA CTATGAGTCC ATCCTGTGGA CTACGGGCAC ACACGCCAGC 
AGTGGTGGTG ATGCTGATGG CTTGGGAGGC ATTGCAGCCC AGGCAGGTTT CAACGCAGGT 
GATGGGCACC GCTACTTCAA CATCCCTGGG TCGCGCACAG CAGACATGGC TGAGGTGGAG 
ACCACCACCA ACGTGGGCGT GCCCGGGCGC TGGGCGTTTA GAATCGATGA TGCCCAGGTG 
CGCGTGGGGG GCTGCGGCCA TACAACCTCT GTGTGCCTAG TCCTGCGTCC GTGCCTCAAT 
GGTGGCAAGT GCATCGATGA CTGTGTCACG GGCAATCCCT CCTACACCTG TTCCTGTCTC 
GCTGGCTTCA CGGGGCGTAG ATGCCACCTG GATGTGAACG AGTGTGCTTC CCACCCATGT 
CAGAACGGTG GGACCTGCAC CCACGGTGTC AACAGCTTCA GTTGCCAGTG CCCAGCCGGC 
TTCCAGGGAC CCACCTGTGA ATCAGCCCAG TCTCCGTGTG ACAACAAAGT GTGTCAAAAT 
GGTGGCCAGT GCCAGGCAGA GAGCAGCTCT GCAGTATGTG TGTGTCAAGC TGGATACACT 
GGGGCCACCT GTGAGACCGA TGTGGATGAA TGCAGCTCTG ACCCATGCCT AAATGGTGGA 
TCGTGTGTTG ACCTGGTTGG AAACTACAGC TGTATTTGTG TGGAGCCCTT TGAAGGACCT 
CAGTGCGAGA CAGGAAGCTA CGTGGTGCCT TCGCCCTGCC TCTCCAACCC CTGCCTGAAC 
GGGGGCACCT GTGTGGATGC TGACCAGGGA TACGTGTGCG AATGCCCTGA AGGTTTCATG 
GGCTTGGACT GCAGAGAGAG AATTCTCAAT GACTGTGATT GCCGGAATGG AGGCAGATGC 
CTGGGTGCCA ACACCACCAT CTGCCAGTGT CCTCCAGGCT TCTTTGGGCT CCTCTGTGAA 
TTTGAAGTCA CAGCCACGCC CTGCAACATG AACACTCAGT GTCCAGATGG AGGCTACTGC 
ATGGAGTATG GCGGAAGCTA CCTATGTGTC TGCCACACGG ACCACAACAT CAGCCATTCT 
CTGCCCTCGC CCTGCGACTC AGACCCATGC TTTAATGGAG GTTCCTGTGA CGCCCACGAG 
GACTCCTACA CGTGCGAGTG CCCTCGTGGA TTCCATGGCA GGCACTGCGA GAAAGCCCGG 
CCACACCTGT GCAGCTCAGG GCCCTGCCGG AATGGGGGCA CATGCAAGGA GACTGGTGAC 
GAGTACCGCT GCACCTGCCC TTACCGGTTC ACTGGGAGAC ACTGTGAGAT TGGAAAGCCA 
GACTCCTGTG CCTCTGGCCC CTGTCACAAC GGTGGCACTT GTTTCCACTA CATTGGCAAA 
TACAAGTGTG ACTGCCCTCC AGGCTTCTCT GGTCGGCACT GTGAGATAGC CCCCTCCCCC 
TGCTTCCGGA GCCCATGTAT GAATGGGGGT ATCTGCGAGG ATCTAGGAAC AGATTTCTCC 
TGCCACTGCC AACCAGGATA TACAGGACAC CGGTGTCAGG CAGAGGTGGA CTGCGGTCAG 
CCTGAGGAGG TAAAACATGC TACCATGCGT CTCAATGGAA CTCGCATGGG CTCGGTGGCC 
CTGTACACAT GTGACCCCGG CTTCAGCCTG AGCGTCCTCA GCCATATGCG TGTCTGTCAG 
CCACAAGGTG TCTGGAGCCA GCCTCCCCAG TGCATTGAAG TAGATGAGTG CCAGTCTCAG 
CCATGCCTGC ATAAAGGCTC CTGCCAGGAC CTCATTGCTG GTTACCAGTG CCTCTGCAGC 
CCGGGGTACG AAGGAGTCCA CTGTGAGCTA GAGACAGACG AGTGCCAAGC ACAGCCCTGC 
AGAAATGGAG GCTCCTGCAG GGACCTCCCC GGGGCTTTCA TCTGCCAGTG CCCTGAAGGT 
TTTGTTGGAA CCCACTGTGA AACAGAGGTG GATGCCTGTG CCTCCAGCCC CTGCCAGCAC 
GGAGGCCGGT GTGAGGACGG TGGTGGGGCC TACCTGTGCG TGTGTCCAGA GGGCTTCTTC 
GGCTACAACT GTGAGACAGT GAGTGACCCC TGCTTCTCTA GCCCCTGTGG GGGCCGCGGC 
TACTGCTTGG CCAGCAACGG GTCCCACAGC TGTACCTGCA AAGTGGGCTA CACAGGCAAG 
GACTGCACCA AAGCCCTCAG GGTAGAAAGG GTGGAGGAGA GTGGGGTCTC CATCTCCTGG 
AGCCCACCCG AGGGCACCAC GGCCAGACAG GTGCTGGACG GCTATGCAGT CACCTATGCC 
TCCTCGGATG GATCGTCCAG GCGCACGGAC TTTGTGGACC GGAGCCGCTC CTCTCACCAG 
CTTCGGGCCC TGGCAGCCGG CCGTGCCTAC AACATCTCTG TTTTCTCAGT CAAGAGAAAC 
ACTAACAACA AAAATGACAT CAGCAGGCCT GCAGCCCTGC TCACCCGCAC CCGACCCCGC 
CCTATTGAAG ACTTCGAGGT CACCAACATT TCAGCCAATG CCATCTCAGT GCAGTGGGCT 
CTTCATAGGA TCCAGCATGC CACTGTCAGC AGGGTTCGAG TGTCTGTCCT CTACCCTGAG 
GACACTGTGG TCCAGTCCAC GGAGGTGGAC AGGAGTGTGG ACCGCCTCAC ATTTGGGGAC 
CTGCTGCCAG GGAGAAGATA CAGTGTGCGG CTAACCACCC TCAGTGGGCC TGGAGGAGCT 
GAATATCCTA CAGAGAGCCT GGCCTCAGCT CCGCTGAACG TGTGGACCCG GCCTTTGCCT 
CCAGCAAACC TGACTGCCTC TCGAGTCACA GCGACCTCTG CCCATATGGT CTGGGACCCG 
CCCACTCCAG GCATCTCACT GGAGGCTTAC GTCATCAATG TGACCACCAG TCAGAATACC 
AAGAGCCGCT ACATCCCCAA TGGGAAGCTG GTGTCCTATA CGGTGCGTGA TCTGATGCCA 
GGTCGGCGGT ACCAGCTCTC GGTCACAGCG GTGCAGAGCA CAGAGCAGGG CCAGCTGCAC 
AGTGAGCCTG CGCACCTCTA CATCATCACC TCCCCCAGGG ATGGCACCGA CAGGCGCTGG 
CACCAGGGAG GACACCACTC ACGAATGCTC AGAAATAGGC CGGCCCCTTT GCGCCTGCCA 
GAACTGCGCC TCCTCAATGA CCACGGTGCC CCTGAAACAC CAACCCAGCC ACCCAGGTTC 
TCAGAGCTTG TAGACGGAAG AGCAAGAGTG AGTGCCAGGT TTGGTGGATT GCCCAGCAGA 
GCAGTAACTG TGAGATCACA ACCCACTACT CCGGTGCCGC TCAAGAACAC AGAGGCCCCT 
GAGCAGGCCC GTCTGGCCCT TCAGCTACCC AAGAACAACA GCAAGGACAC AGAAAGTACC 
CCTGGCAGCT GTTCAGAAGA CACCTGTCAG AATGGAGGCA CCTGTGTCCC AGGTGCCAAT 
GCCCACAGCT GTGACTGCAG GCCTGGGTTC AAAGGCAGAC ACTGTGAGCT TGCCTGTGAA 
AAAGTGCCCC GCCCCTGCAC ACGGCTGTTC TCTGAGACCA AGTCATTTCC TGTCTGGGAA 
GGAGATGTCT GCCACCATGT GTATAAGAAA GTCTACAAAG TTCACCAGGA CGTGTGTTTT 
AAGGAGCGCT GCCAGAGCAC AAGCCTCAAA AAGCTCAAAC AGGAATCAAA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sned1 ( XM_006245526.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006245588.1 Rattus norvegicus sushi, nidogen and EGF-like domains 1 (Sned1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ORa32574
Clone ID Related Accession (Same CDS sequence)	XM_006245526.3
Accession Version	XM_006245526.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4212bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	sushi, nidogen and EGF-like domain-containing protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005108.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006245526.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGCCGTG GCGCCGCCTG GGCGCTGCTG CTGGCCGCAG CCCTGGGGCT CGGGGCGCGC 
GGGGTGCGCG CTGCGGTGGC CCTCGCCGAC TTCTACCCGT TCGGCACGAA GCGTGGCGAC 
GCCGTCACCC CGAAGCAGGA CGACGGCGGC TCAGGGCTGC AGCCCCTCTC GGTGCCCTTC 
CCGTTCTTCG GCGCCGAGCA CTCAGGACTC TACGTGAACA ACAACGGGAT CATCTCCTTC 
CTGAAGGAAG TTTCTCAGTT CACCCCTGTG GCCTTCCCCA TCGCCAAAGA CCGCTGTGTG 
GTAGCGGCCT TCTGGGCAGA CGTGGATAAC CGACGCGCAG GCGACGTCTA CTACCGGGAG 
GCCACCGACG CAGCCATGCT GAACAGAGCC ACGGAGGACA TCAGGCGGTA CTTTCCTGAG 
CTCCCGGACT TCTCTGCTAC CTGGGTTTTT GTTGCCACCT GGTACCGTGT GACCTTCTTC 
GGAGGCAGCA GCTCTTCCCC CGTCAACACA TTCCAAACGG TGCTCATCAC TGACGGCCGG 
TTCTCCTTCA CCATCTTCAA CTATGAGTCC ATCCTGTGGA CTACGGGCAC ACACGCCAGC 
AGTGGTGGTG ATGCTGATGG CTTGGGAGGC ATTGCAGCCC AGGCAGGTTT CAACGCAGGT 
GATGGGCACC GCTACTTCAA CATCCCTGGG TCGCGCACAG CAGACATGGC TGAGGTGGAG 
ACCACCACCA ACGTGGGCGT GCCCGGGCGC TGGGCGTTTA GAATCGATGA TGCCCAGGTG 
CGCGTGGGGG GCTGCGGCCA TACAACCTCT GTGTGCCTAG TCCTGCGTCC GTGCCTCAAT 
GGTGGCAAGT GCATCGATGA CTGTGTCACG GGCAATCCCT CCTACACCTG TTCCTGTCTC 
GCTGGCTTCA CGGGGCGTAG ATGCCACCTG GATGTGAACG AGTGTGCTTC CCACCCATGT 
CAGAACGGTG GGACCTGCAC CCACGGTGTC AACAGCTTCA GTTGCCAGTG CCCAGCCGGC 
TTCCAGGGAC CCACCTGTGA ATCAGCCCAG TCTCCGTGTG ACAACAAAGT GTGTCAAAAT 
GGTGGCCAGT GCCAGGCAGA GAGCAGCTCT GCAGTATGTG TGTGTCAAGC TGGATACACT 
GGGGCCACCT GTGAGACCGA TGTGGATGAA TGCAGCTCTG ACCCATGCCT AAATGGTGGA 
TCGTGTGTTG ACCTGGTTGG AAACTACAGC TGTATTTGTG TGGAGCCCTT TGAAGGACCT 
CAGTGCGAGA CAGGAAGCTA CGTGGTGCCT TCGCCCTGCC TCTCCAACCC CTGCCTGAAC 
GGGGGCACCT GTGTGGATGC TGACCAGGGA TACGTGTGCG AATGCCCTGA AGGTTTCATG 
GGCTTGGACT GCAGAGAGAG AATTCTCAAT GACTGCGATT GCCGGAATGG AGGCAGATGC 
CTGGGTGCCA ACACCACCAT CTGCCAGTGT CCTCCAGGCT TCTTTGGGCT CCTCTGTGAA 
TTTGAAGTCA CAGCCACGCC CTGCAACATG AACACTCAGT GTCCAGATGG AGGCTACTGC 
ATGGAGTATG GCGGAAGCTA CCTATGTGTC TGCCACACGG ACCACAACAT CAGCCATTCT 
CTGCCCTCGC CCTGCGACTC AGACCCATGC TTTAATGGAG GTTCCTGTGA CGCCCACGAG 
GACTCCTACA CGTGCGAGTG CCCTCGTGGA TTCCATGGCA GGCACTGCGA GAAAGCCCGG 
CCACACCTGT GCAGCTCAGG GCCCTGCCGG AATGGGGGCA CATGCAAGGA GACTGGTGAC 
GAGTACCGCT GCACCTGCCC TTACCGGTTC ACTGGGAGAC ACTGTGAGAT TGGAAAGCCA 
GACTCCTGTG CCTCTGGCCC CTGTCACAAC GGTGGCACTT GTTTCCACTA CATTGGCAAA 
TACAAGTGTG ACTGCCCTCC AGGCTTCTCT GGTCGGCACT GTGAGATAGC CCCCTCCCCC 
TGCTTCCGGA GCCCATGTAT GAATGGGGGT ATCTGCGAGG ATCTAGGAAC AGATTTCTCC 
TGCCACTGCC AACCAGGATA TACAGGACAC CGGTGTCAGG CAGAGGTGGA CTGCGGTCAG 
CCTGAGGAGG TAAAACATGC TACCATGCGT CTCAATGGAA CTCGCATGGG CTCGGTGGCC 
CTGTACACAT GTGACCCCGG CTTCAGCCTG AGCGTCCTCA GCCATATGCG TGTCTGTCAG 
CCACAAGGTG TCTGGAGCCA GCCTCCCCAG TGCATTGAAG TAGATGAGTG CCAGTCTCAG 
CCATGCCTGC ATAAAGGCTC CTGCCAGGAC CTCATTGCTG GTTACCAGTG CCTCTGCAGC 
CCGGGGTACG AAGGAGTCCA CTGTGAGCTA GAGACAGACG AGTGCCAAGC ACAGCCCTGC 
AGAAATGGAG GCTCCTGCAG GGACCTCCCC GGGGCTTTCA TCTGCCAGTG CCCTGAAGGT 
TTTGTTGGAA CCCACTGTGA AACAGAGGTG GATGCCTGTG CCTCCAGCCC CTGCCAGCAC 
GGAGGCCGGT GTGAGGACGG TGGTGGGGCC TACCTGTGCG TGTGTCCAGA GGGCTTCTTC 
GGCTACAACT GTGAGACAGT GAGTGACCCC TGCTTCTCTA GCCCCTGTGG GGGCCGCGGC 
TACTGCTTGG CCAGCAACGG GTCCCACAGC TGTACCTGCA AAGTGGGCTA CACAGGCAAG 
GACTGCACCA AAGAGCTCCT CCCACCAACA GCCCTCAGGG TAGAAAGGGT GGAGGAGAGT 
GGGGTCTCCA TCTCCTGGAG CCCACCCGAG GGCACCACGG CCAGACAGGT GCTGGACGGC 
TATGCAGTCA CCTATGCCTC CTCGGATGGA TCGTCCAGGC GCACGGACTT TGTGGACCGG 
AGCCGCTCCT CTCACCAGCT TCGGGCCCTG GCAGCCGGCC GTGCCTACAA CATCTCTGTT 
TTCTCAGTCA AGAGAAACAC TAACAACAAA AATGACATCA GCAGGCCTGC AGCCCTGCTC 
ACCCGCACCC GACCCCGCCC TATTGAAGAC TTCGAGGTCA CCAACATTTC AGCCAATGCC 
ATCTCAGTGC AGTGGGCTCT TCATAGGATC CAGCATGCCA CTGTCAGCAG GGTTCGAGTG 
TCTGTCCTCT ACCCTGAGGA CACTGTGGTC CAGTCCACGG AGGTGGACAG GAGTGTGGAC 
CGCCTCACAT TTGGGGACCT GCTGCCAGGG AGAAGATACA GTGTGCGGCT AACCACCCTC 
AGTGGGCCTG GAGGAGCTGA ATATCCTACA GAGAGCCTGG CCTCAGCTCC GCTGAACGTG 
TGGACCCGGC CTTTGCCTCC AGCAAACCTG ACTGCCTCTC GAGTCACAGC GACCTCTGCC 
CATATGGTCT GGGACCCGCC CACTCCAGGC ATCTCACTGG AGGCTTACGT CATCAATGTG 
ACCACCAGTC AGAATACCAA GAGCCGCTAC ATCCCCAATG GGAAGCTGGT GTCCTATACG 
GTGCGTGATC TGATGCCAGG TCGGCGGTAC CAGCTCTCGG TCACAGCGGT GCAGAGCACA 
GAGCAGGGCC AGCTGCACAG TGAGCCTGCG CACCTCTACA TCATCACCTC CCCCAGGGAT 
GGCACCGACA GGCGCTGGCA CCAGGGAGGA CACCACTCAC GAATGCTCAG AAATAGGCCG 
GCCCCTTTGC GCCTGCCAGA ACTGCGCCTC CTCAATGACC ACGGTGCCCC TGAAACACCA 
ACCCAGCCAC CCAGGTTCTC AGAGCTTGTA GACGGAAGAG CAAGAGTGAG TGCCAGGTTT 
GGTGGATTGC CCAGCAGAGC AGTAACTGTG AGATCACAAC CCACTACTCC GGTGCCGCTC 
AAGAACACAG AGGCCCCTGA GCAGGCCCGT CTGGCCCTTC AGCTACCCAA GAACAACAGC 
AAGGACACAG AAAGTACCCC TGGCAGCTGT TCAGAAGACA CCTGTCAGAA TGGAGGCACC 
TGTGTCCCAG GTGCCAATGC CCACAGCTGT GACTGCAGGC CTGGGTTCAA AGGCAGACAC 
TGTGAGCTTG CCTGTGAAAA AGTGCCCCGC CCCTGCACAC GGCTGTTCTC TGAGACCAAG 
TCATTTCCTG TCTGGGAAGG AGATGTCTGC CACCATGTGT ATAAGAAAGT CTACAAAGTT 
CACCAGGACG TGTGTTTTAA GGAGCGCTGC CAGAGCACAA GCCTCAAAAA GCTCAAACAG 
GAATCAAATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006245588.1
CDS	178..4389
Translation	MRRGAAWALLLAAALGLGARGVRAAVALADFYPFGTKRGDAVTPKQDDGGSGLQPLSVPFPFFGAEHSGLYVNNNGIISFLKEVSQFTPVAFPIAKDRCVVAAFWADVDNRRAGDVYYREATDAAMLNRATEDIRRYFPELPDFSATWVFVATWYRVTFFGGSSSSPVNTFQTVLITDGRFSFTIFNYESILWTTGTHASSGGDADGLGGIAAQAGFNAGDGHRYFNIPGSRTADMAEVETTTNVGVPGRWAFRIDDAQVRVGGCGHTTSVCLVLRPCLNGGKCIDDCVTGNPSYTCSCLAGFTGRRCHLDVNECASHPCQNGGTCTHGVNSFSCQCPAGFQGPTCESAQSPCDNKVCQNGGQCQAESSSAVCVCQAGYTGATCETDVDECSSDPCLNGGSCVDLVGNYSCICVEPFEGPQCETGSYVVPSPCLSNPCLNGGTCVDADQGYVCECPEGFMGLDCRERILNDCDCRNGGRCLGANTTICQCPPGFFGLLCEFEVTATPCNMNTQCPDGGYCMEYGGSYLCVCHTDHNISHSLPSPCDSDPCFNGGSCDAHEDSYTCECPRGFHGRHCEKARPHLCSSGPCRNGGTCKETGDEYRCTCPYRFTGRHCEIGKPDSCASGPCHNGGTCFHYIGKYKCDCPPGFSGRHCEIAPSPCFRSPCMNGGICEDLGTDFSCHCQPGYTGHRCQAEVDCGQPEEVKHATMRLNGTRMGSVALYTCDPGFSLSVLSHMRVCQPQGVWSQPPQCIEVDECQSQPCLHKGSCQDLIAGYQCLCSPGYEGVHCELETDECQAQPCRNGGSCRDLPGAFICQCPEGFVGTHCETEVDACASSPCQHGGRCEDGGGAYLCVCPEGFFGYNCETVSDPCFSSPCGGRGYCLASNGSHSCTCKVGYTGKDCTKELLPPTALRVERVEESGVSISWSPPEGTTARQVLDGYAVTYASSDGSSRRTDFVDRSRSSHQLRALAAGRAYNISVFSVKRNTNNKNDISRPAALLTRTRPRPIEDFEVTNISANAISVQWALHRIQHATVSRVRVSVLYPEDTVVQSTEVDRSVDRLTFGDLLPGRRYSVRLTTLSGPGGAEYPTESLASAPLNVWTRPLPPANLTASRVTATSAHMVWDPPTPGISLEAYVINVTTSQNTKSRYIPNGKLVSYTVRDLMPGRRYQLSVTAVQSTEQGQLHSEPAHLYIITSPRDGTDRRWHQGGHHSRMLRNRPAPLRLPELRLLNDHGAPETPTQPPRFSELVDGRARVSARFGGLPSRAVTVRSQPTTPVPLKNTEAPEQARLALQLPKNNSKDTESTPGSCSEDTCQNGGTCVPGANAHSCDCRPGFKGRHCELACEKVPRPCTRLFSETKSFPVWEGDVCHHVYKKVYKVHQDVCFKERCQSTSLKKLKQESN

Target ORF information:

RefSeq Version	XM_006245526.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus sushi, nidogen and EGF-like domains 1 (Sned1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006245526.3

ORF Insert Sequence:

ATGCGCCGTG GCGCCGCCTG GGCGCTGCTG CTGGCCGCAG CCCTGGGGCT CGGGGCGCGC 
GGGGTGCGCG CTGCGGTGGC CCTCGCCGAC TTCTACCCGT TCGGCACGAA GCGTGGCGAC 
GCCGTCACCC CGAAGCAGGA CGACGGCGGC TCAGGGCTGC AGCCCCTCTC GGTGCCCTTC 
CCGTTCTTCG GCGCCGAGCA CTCAGGACTC TACGTGAACA ACAACGGGAT CATCTCCTTC 
CTGAAGGAAG TTTCTCAGTT CACCCCTGTG GCCTTCCCCA TCGCCAAAGA CCGCTGTGTG 
GTAGCGGCCT TCTGGGCAGA CGTGGATAAC CGACGCGCAG GCGACGTCTA CTACCGGGAG 
GCCACCGACG CAGCCATGCT GAACAGAGCC ACGGAGGACA TCAGGCGGTA CTTTCCTGAG 
CTCCCGGACT TCTCTGCTAC CTGGGTTTTT GTTGCCACCT GGTACCGTGT GACCTTCTTC 
GGAGGCAGCA GCTCTTCCCC CGTCAACACA TTCCAAACGG TGCTCATCAC TGACGGCCGG 
TTCTCCTTCA CCATCTTCAA CTATGAGTCC ATCCTGTGGA CTACGGGCAC ACACGCCAGC 
AGTGGTGGTG ATGCTGATGG CTTGGGAGGC ATTGCAGCCC AGGCAGGTTT CAACGCAGGT 
GATGGGCACC GCTACTTCAA CATCCCTGGG TCGCGCACAG CAGACATGGC TGAGGTGGAG 
ACCACCACCA ACGTGGGCGT GCCCGGGCGC TGGGCGTTTA GAATCGATGA TGCCCAGGTG 
CGCGTGGGGG GCTGCGGCCA TACAACCTCT GTGTGCCTAG TCCTGCGTCC GTGCCTCAAT 
GGTGGCAAGT GCATCGATGA CTGTGTCACG GGCAATCCCT CCTACACCTG TTCCTGTCTC 
GCTGGCTTCA CGGGGCGTAG ATGCCACCTG GATGTGAACG AGTGTGCTTC CCACCCATGT 
CAGAACGGTG GGACCTGCAC CCACGGTGTC AACAGCTTCA GTTGCCAGTG CCCAGCCGGC 
TTCCAGGGAC CCACCTGTGA ATCAGCCCAG TCTCCGTGTG ACAACAAAGT GTGTCAAAAT 
GGTGGCCAGT GCCAGGCAGA GAGCAGCTCT GCAGTATGTG TGTGTCAAGC TGGATACACT 
GGGGCCACCT GTGAGACCGA TGTGGATGAA TGCAGCTCTG ACCCATGCCT AAATGGTGGA 
TCGTGTGTTG ACCTGGTTGG AAACTACAGC TGTATTTGTG TGGAGCCCTT TGAAGGACCT 
CAGTGCGAGA CAGGAAGCTA CGTGGTGCCT TCGCCCTGCC TCTCCAACCC CTGCCTGAAC 
GGGGGCACCT GTGTGGATGC TGACCAGGGA TACGTGTGCG AATGCCCTGA AGGTTTCATG 
GGCTTGGACT GCAGAGAGAG AATTCTCAAT GACTGCGATT GCCGGAATGG AGGCAGATGC 
CTGGGTGCCA ACACCACCAT CTGCCAGTGT CCTCCAGGCT TCTTTGGGCT CCTCTGTGAA 
TTTGAAGTCA CAGCCACGCC CTGCAACATG AACACTCAGT GTCCAGATGG AGGCTACTGC 
ATGGAGTATG GCGGAAGCTA CCTATGTGTC TGCCACACGG ACCACAACAT CAGCCATTCT 
CTGCCCTCGC CCTGCGACTC AGACCCATGC TTTAATGGAG GTTCCTGTGA CGCCCACGAG 
GACTCCTACA CGTGCGAGTG CCCTCGTGGA TTCCATGGCA GGCACTGCGA GAAAGCCCGG 
CCACACCTGT GCAGCTCAGG GCCCTGCCGG AATGGGGGCA CATGCAAGGA GACTGGTGAC 
GAGTACCGCT GCACCTGCCC TTACCGGTTC ACTGGGAGAC ACTGTGAGAT TGGAAAGCCA 
GACTCCTGTG CCTCTGGCCC CTGTCACAAC GGTGGCACTT GTTTCCACTA CATTGGCAAA 
TACAAGTGTG ACTGCCCTCC AGGCTTCTCT GGTCGGCACT GTGAGATAGC CCCCTCCCCC 
TGCTTCCGGA GCCCATGTAT GAATGGGGGT ATCTGCGAGG ATCTAGGAAC AGATTTCTCC 
TGCCACTGCC AACCAGGATA TACAGGACAC CGGTGTCAGG CAGAGGTGGA CTGCGGTCAG 
CCTGAGGAGG TAAAACATGC TACCATGCGT CTCAATGGAA CTCGCATGGG CTCGGTGGCC 
CTGTACACAT GTGACCCCGG CTTCAGCCTG AGCGTCCTCA GCCATATGCG TGTCTGTCAG 
CCACAAGGTG TCTGGAGCCA GCCTCCCCAG TGCATTGAAG TAGATGAGTG CCAGTCTCAG 
CCATGCCTGC ATAAAGGCTC CTGCCAGGAC CTCATTGCTG GTTACCAGTG CCTCTGCAGC 
CCGGGGTACG AAGGAGTCCA CTGTGAGCTA GAGACAGACG AGTGCCAAGC ACAGCCCTGC 
AGAAATGGAG GCTCCTGCAG GGACCTCCCC GGGGCTTTCA TCTGCCAGTG CCCTGAAGGT 
TTTGTTGGAA CCCACTGTGA AACAGAGGTG GATGCCTGTG CCTCCAGCCC CTGCCAGCAC 
GGAGGCCGGT GTGAGGACGG TGGTGGGGCC TACCTGTGCG TGTGTCCAGA GGGCTTCTTC 
GGCTACAACT GTGAGACAGT GAGTGACCCC TGCTTCTCTA GCCCCTGTGG GGGCCGCGGC 
TACTGCTTGG CCAGCAACGG GTCCCACAGC TGTACCTGCA AAGTGGGCTA CACAGGCAAG 
GACTGCACCA AAGAGCTCCT CCCACCAACA GCCCTCAGGG TAGAAAGGGT GGAGGAGAGT 
GGGGTCTCCA TCTCCTGGAG CCCACCCGAG GGCACCACGG CCAGACAGGT GCTGGACGGC 
TATGCAGTCA CCTATGCCTC CTCGGATGGA TCGTCCAGGC GCACGGACTT TGTGGACCGG 
AGCCGCTCCT CTCACCAGCT TCGGGCCCTG GCAGCCGGCC GTGCCTACAA CATCTCTGTT 
TTCTCAGTCA AGAGAAACAC TAACAACAAA AATGACATCA GCAGGCCTGC AGCCCTGCTC 
ACCCGCACCC GACCCCGCCC TATTGAAGAC TTCGAGGTCA CCAACATTTC AGCCAATGCC 
ATCTCAGTGC AGTGGGCTCT TCATAGGATC CAGCATGCCA CTGTCAGCAG GGTTCGAGTG 
TCTGTCCTCT ACCCTGAGGA CACTGTGGTC CAGTCCACGG AGGTGGACAG GAGTGTGGAC 
CGCCTCACAT TTGGGGACCT GCTGCCAGGG AGAAGATACA GTGTGCGGCT AACCACCCTC 
AGTGGGCCTG GAGGAGCTGA ATATCCTACA GAGAGCCTGG CCTCAGCTCC GCTGAACGTG 
TGGACCCGGC CTTTGCCTCC AGCAAACCTG ACTGCCTCTC GAGTCACAGC GACCTCTGCC 
CATATGGTCT GGGACCCGCC CACTCCAGGC ATCTCACTGG AGGCTTACGT CATCAATGTG 
ACCACCAGTC AGAATACCAA GAGCCGCTAC ATCCCCAATG GGAAGCTGGT GTCCTATACG 
GTGCGTGATC TGATGCCAGG TCGGCGGTAC CAGCTCTCGG TCACAGCGGT GCAGAGCACA 
GAGCAGGGCC AGCTGCACAG TGAGCCTGCG CACCTCTACA TCATCACCTC CCCCAGGGAT 
GGCACCGACA GGCGCTGGCA CCAGGGAGGA CACCACTCAC GAATGCTCAG AAATAGGCCG 
GCCCCTTTGC GCCTGCCAGA ACTGCGCCTC CTCAATGACC ACGGTGCCCC TGAAACACCA 
ACCCAGCCAC CCAGGTTCTC AGAGCTTGTA GACGGAAGAG CAAGAGTGAG TGCCAGGTTT 
GGTGGATTGC CCAGCAGAGC AGTAACTGTG AGATCACAAC CCACTACTCC GGTGCCGCTC 
AAGAACACAG AGGCCCCTGA GCAGGCCCGT CTGGCCCTTC AGCTACCCAA GAACAACAGC 
AAGGACACAG AAAGTACCCC TGGCAGCTGT TCAGAAGACA CCTGTCAGAA TGGAGGCACC 
TGTGTCCCAG GTGCCAATGC CCACAGCTGT GACTGCAGGC CTGGGTTCAA AGGCAGACAC 
TGTGAGCTTG CCTGTGAAAA AGTGCCCCGC CCCTGCACAC GGCTGTTCTC TGAGACCAAG 
TCATTTCCTG TCTGGGAAGG AGATGTCTGC CACCATGTGT ATAAGAAAGT CTACAAAGTT 
CACCAGGACG TGTGTTTTAA GGAGCGCTGC CAGAGCACAA GCCTCAAAAA GCTCAAACAG 
GAATCAAATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sned1 ( XM_008767373.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008765595.1 Rattus norvegicus sushi, nidogen and EGF-like domains 1 (Sned1), transcript variant X1, mRNA.

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CloneID	ORa47568
Clone ID Related Accession (Same CDS sequence)	XM_008767373.2
Accession Version	XM_008767373.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4515bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	sushi, nidogen and EGF-like domain-containing protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005108.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008767373.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGCCGTG GCGCCGCCTG GGCGCTGCTG CTGGCCGCAG CCCTGGGGCT CGGGGCGCGC 
GGGGTGCGCG CTGCGGTGGC CCTCGCCGAC TTCTACCCGT TCGGCACGAA GCGTGGCGAC 
GCCGTCACCC CGAAGCAGGA CGACGGCGGC TCAGGGCTGC AGCCCCTCTC GGTGCCCTTC 
CCGTTCTTCG GCGCCGAGCA CTCAGGACTC TACGTGAACA ACAACGGGAT CATCTCCTTC 
CTGAAGGAAG TTTCTCAGTT CACCCCTGTG GCCTTCCCCA TCGCCAAAGA CCGCTGTGTG 
GTAGCGGCCT TCTGGGCAGA CGTGGATAAC CGACGCGCAG GCGACGTCTA CTACCGGGAG 
GCCACCGACG CAGCCATGCT GAACAGAGCC ACGGAGGACA TCAGGCGGTA CTTTCCTGAG 
CTCCCGGACT TCTCTGCTAC CTGGGTTTTT GTTGCCACCT GGTACCGTGT GACCTTCTTC 
GGAGGCAGCA GCTCTTCCCC CGTCAACACA TTCCAAACGG TGCTCATCAC TGACGGCCGG 
TTCTCCTTCA CCATCTTCAA CTATGAGTCC ATCCTGTGGA CTACGGGCAC ACACGCCAGC 
AGTGGTGGTG ATGCTGATGG CTTGGGAGGC ATTGCAGCCC AGGCAGGTTT CAACGCAGGT 
GATGGGCACC GCTACTTCAA CATCCCTGGG TCGCGCACAG CAGACATGGC TGAGGTGGAG 
ACCACCACCA ACGTGGGCGT GCCCGGGCGC TGGGCGTTTA GAATCGATGA TGCCCAGGTG 
CGCGTGGGGG GCTGCGGCCA TACAACCTCT GTGTGCCTAG TCCTGCGTCC GTGCCTCAAT 
GGTGGCAAGT GCATCGATGA CTGTGTCACG GGCAATCCCT CCTACACCTG TTCCTGTCTC 
GCTGGCTTCA CGGGGCGTAG ATGCCACCTG GATGTGAACG AGTGTGCTTC CCACCCATGT 
CAGAACGGTG GGACCTGCAC CCACGGTGTC AACAGCTTCA GTTGCCAGTG CCCAGCCGGC 
TTCCAGGGAC CCACCTGTGA ATCAGCCCAG TCTCCGTGTG ACAACAAAGT GTGTCAAAAT 
GGTGGCCAGT GCCAGGCAGA GAGCAGCTCT GCAGTATGTG TGTGTCAAGC TGGATACACT 
GGGGCCACCT GTGAGACCGA TGTGGATGAA TGCAGCTCTG ACCCATGCCT AAATGGTGGA 
TCGTGTGTTG ACCTGGTTGG AAACTACAGC TGTATTTGTG TGGAGCCCTT TGAAGGACCT 
CAGTGCGAGA CAGGAAGCTA CGTGGTGCCT TCGCCCTGCC TCTCCAACCC CTGCCTGAAC 
GGGGGCACCT GTGTGGATGC TGACCAGGGA TACGTGTGCG AATGCCCTGA AGGTTTCATG 
GGCTTGGACT GCAGAGAGAG AATTCTCAAT GACTGCGATT GCCGGAATGG AGGCAGATGC 
CTGGGTGCCA ACACCACCAT CTGCCAGTGT CCTCCAGGCT TCTTTGGGCT CCTCTGTGAA 
TTTGAAGTCA CAGCCACGCC CTGCAACATG AACACTCAGT GTCCAGATGG AGGCTACTGC 
ATGGAGTATG GCGGAAGCTA CCTATGTGTC TGCCACACGG ACCACAACAT CAGCCATTCT 
CTGCCCTCGC CCTGCGACTC AGACCCATGC TTTAATGGAG GTTCCTGTGA CGCCCACGAG 
GACTCCTACA CGTGCGAGTG CCCTCGTGGA TTCCATGGCA GGCACTGCGA GAAAGCCCGG 
CCACACCTGT GCAGCTCAGG GCCCTGCCGG AATGGGGGCA CATGCAAGGA GACTGGTGAC 
GAGTACCGCT GCACCTGCCC TTACCGGTTC ACTGGGAGAC ACTGTGAGAT TGGAAAGCCA 
GACTCCTGTG CCTCTGGCCC CTGTCACAAC GGTGGCACTT GTTTCCACTA CATTGGCAAA 
TACAAGTGTG ACTGCCCTCC AGGCTTCTCT GGTCGGCACT GTGAGATAGC CCCCTCCCCC 
TGCTTCCGGA GCCCATGTAT GAATGGGGGT ATCTGCGAGG ATCTAGGAAC AGATTTCTCC 
TGCCACTGCC AACCAGGATA TACAGGACAC CGGTGTCAGG CAGAGGTGGA CTGCGGTCAG 
CCTGAGGAGG TAAAACATGC TACCATGCGT CTCAATGGAA CTCGCATGGG CTCGGTGGCC 
CTGTACACAT GTGACCCCGG CTTCAGCCTG AGCGTCCTCA GCCATATGCG TGTCTGTCAG 
CCACAAGGTG TCTGGAGCCA GCCTCCCCAG TGCATTGAAG TAGATGAGTG CCAGTCTCAG 
CCATGCCTGC ATAAAGGCTC CTGCCAGGAC CTCATTGCTG GTTACCAGTG CCTCTGCAGC 
CCGGGGTACG AAGGAGTCCA CTGTGAGCTA GAGACAGACG AGTGCCAAGC ACAGCCCTGC 
AGAAATGGAG GCTCCTGCAG GGACCTCCCC GGGGCTTTCA TCTGCCAGTG CCCTGAAGGT 
TTTGTTGGAA CCCACTGTGA AACAGGTAGG GTTCTTTCAG GAGGGTCCCA CAGAACCAGG 
GCTGCTGGGA GATCAGAGGG GAAGCCAGGA GCCATCCTCT TGCTGGACAA GCCACCCAGA 
GAGAGAAACC CCCAAGGCTG TGGTGACCTG GAGGGAGGAA GGAAACTGGG CGGGGGGTCC 
CTGAGAGCCC CTCCTACCCA CAGGTGTGCC ATACCCCTCT CCTGGCCATT GCTCCTTGCC 
TCCCCCTATC CCGTCTTCCC GCTGTCCTCT CCTCCCTCCC CCAGAGTCCC TCCCTGCAGC 
TCGGGCCAAC CCTTTGGGTG TTCTCCAGAG GTGGATGCCT GTGCCTCCAG CCCCTGCCAG 
CACGGAGGCC GGTGTGAGGA CGGTGGTGGG GCCTACCTGT GCGTGTGTCC AGAGGGCTTC 
TTCGGCTACA ACTGTGAGAC AGTGAGTGAC CCCTGCTTCT CTAGCCCCTG TGGGGGCCGC 
GGCTACTGCT TGGCCAGCAA CGGGTCCCAC AGCTGTACCT GCAAAGTGGG CTACACAGGC 
AAGGACTGCA CCAAAGAGCT CCTCCCACCA ACAGCCCTCA GGGTAGAAAG GGTGGAGGAG 
AGTGGGGTCT CCATCTCCTG GAGCCCACCC GAGGGCACCA CGGCCAGACA GGTGCTGGAC 
GGCTATGCAG TCACCTATGC CTCCTCGGAT GGATCGTCCA GGCGCACGGA CTTTGTGGAC 
CGGAGCCGCT CCTCTCACCA GCTTCGGGCC CTGGCAGCCG GCCGTGCCTA CAACATCTCT 
GTTTTCTCAG TCAAGAGAAA CACTAACAAC AAAAATGACA TCAGCAGGCC TGCAGCCCTG 
CTCACCCGCA CCCGACCCCG CCCTATTGAA GACTTCGAGG TCACCAACAT TTCAGCCAAT 
GCCATCTCAG TGCAGTGGGC TCTTCATAGG ATCCAGCATG CCACTGTCAG CAGGGTTCGA 
GTGTCTGTCC TCTACCCTGA GGACACTGTG GTCCAGTCCA CGGAGGTGGA CAGGAGTGTG 
GACCGCCTCA CATTTGGGGA CCTGCTGCCA GGGAGAAGAT ACAGTGTGCG GCTAACCACC 
CTCAGTGGGC CTGGAGGAGC TGAATATCCT ACAGAGAGCC TGGCCTCAGC TCCGCTGAAC 
GTGTGGACCC GGCCTTTGCC TCCAGCAAAC CTGACTGCCT CTCGAGTCAC AGCGACCTCT 
GCCCATATGG TCTGGGACCC GCCCACTCCA GGCATCTCAC TGGAGGCTTA CGTCATCAAT 
GTGACCACCA GTCAGAATAC CAAGAGCCGC TACATCCCCA ATGGGAAGCT GGTGTCCTAT 
ACGGTGCGTG ATCTGATGCC AGGTCGGCGG TACCAGCTCT CGGTCACAGC GGTGCAGAGC 
ACAGAGCAGG GCCAGCTGCA CAGTGAGCCT GCGCACCTCT ACATCATCAC CTCCCCCAGG 
GATGGCACCG ACAGGCGCTG GCACCAGGGA GGACACCACT CACGAATGCT CAGAAATAGG 
CCGGCCCCTT TGCGCCTGCC AGAACTGCGC CTCCTCAATG ACCACGGTGC CCCTGAAACA 
CCAACCCAGC CACCCAGGTT CTCAGAGCTT GTAGACGGAA GAGCAAGAGT GAGTGCCAGG 
TTTGGTGGAT TGCCCAGCAG AGCAGTAACT GTGAGATCAC AACCCACTAC TCCGGTGCCG 
CTCAAGAACA CAGAGGCCCC TGAGCAGGCC CGTCTGGCCC TTCAGCTACC CAAGAACAAC 
AGCAAGGACA CAGAAAGTAC CCCTGGCAGC TGTTCAGAAG ACACCTGTCA GAATGGAGGC 
ACCTGTGTCC CAGGTGCCAA TGCCCACAGC TGTGACTGCA GGCCTGGGTT CAAAGGCAGA 
CACTGTGAGC TTGCCTGTGA AAAAGTGCCC CGCCCCTGCA CACGGCTGTT CTCTGAGACC 
AAGTCATTTC CTGTCTGGGA AGGAGATGTC TGCCACCATG TGTATAAGAA AGTCTACAAA 
GTTCACCAGG ACGTGTGTTT TAAGGAGCGC TGCCAGAGCA CAAGCCTCAA AAAGCTCAAA 
CAGGAATCAA ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008765595.1
CDS	180..4694
Translation	MRRGAAWALLLAAALGLGARGVRAAVALADFYPFGTKRGDAVTPKQDDGGSGLQPLSVPFPFFGAEHSGLYVNNNGIISFLKEVSQFTPVAFPIAKDRCVVAAFWADVDNRRAGDVYYREATDAAMLNRATEDIRRYFPELPDFSATWVFVATWYRVTFFGGSSSSPVNTFQTVLITDGRFSFTIFNYESILWTTGTHASSGGDADGLGGIAAQAGFNAGDGHRYFNIPGSRTADMAEVETTTNVGVPGRWAFRIDDAQVRVGGCGHTTSVCLVLRPCLNGGKCIDDCVTGNPSYTCSCLAGFTGRRCHLDVNECASHPCQNGGTCTHGVNSFSCQCPAGFQGPTCESAQSPCDNKVCQNGGQCQAESSSAVCVCQAGYTGATCETDVDECSSDPCLNGGSCVDLVGNYSCICVEPFEGPQCETGSYVVPSPCLSNPCLNGGTCVDADQGYVCECPEGFMGLDCRERILNDCDCRNGGRCLGANTTICQCPPGFFGLLCEFEVTATPCNMNTQCPDGGYCMEYGGSYLCVCHTDHNISHSLPSPCDSDPCFNGGSCDAHEDSYTCECPRGFHGRHCEKARPHLCSSGPCRNGGTCKETGDEYRCTCPYRFTGRHCEIGKPDSCASGPCHNGGTCFHYIGKYKCDCPPGFSGRHCEIAPSPCFRSPCMNGGICEDLGTDFSCHCQPGYTGHRCQAEVDCGQPEEVKHATMRLNGTRMGSVALYTCDPGFSLSVLSHMRVCQPQGVWSQPPQCIEVDECQSQPCLHKGSCQDLIAGYQCLCSPGYEGVHCELETDECQAQPCRNGGSCRDLPGAFICQCPEGFVGTHCETGRVLSGGSHRTRAAGRSEGKPGAILLLDKPPRERNPQGCGDLEGGRKLGGGSLRAPPTHRCAIPLSWPLLLASPYPVFPLSSPPSPRVPPCSSGQPFGCSPEVDACASSPCQHGGRCEDGGGAYLCVCPEGFFGYNCETVSDPCFSSPCGGRGYCLASNGSHSCTCKVGYTGKDCTKELLPPTALRVERVEESGVSISWSPPEGTTARQVLDGYAVTYASSDGSSRRTDFVDRSRSSHQLRALAAGRAYNISVFSVKRNTNNKNDISRPAALLTRTRPRPIEDFEVTNISANAISVQWALHRIQHATVSRVRVSVLYPEDTVVQSTEVDRSVDRLTFGDLLPGRRYSVRLTTLSGPGGAEYPTESLASAPLNVWTRPLPPANLTASRVTATSAHMVWDPPTPGISLEAYVINVTTSQNTKSRYIPNGKLVSYTVRDLMPGRRYQLSVTAVQSTEQGQLHSEPAHLYIITSPRDGTDRRWHQGGHHSRMLRNRPAPLRLPELRLLNDHGAPETPTQPPRFSELVDGRARVSARFGGLPSRAVTVRSQPTTPVPLKNTEAPEQARLALQLPKNNSKDTESTPGSCSEDTCQNGGTCVPGANAHSCDCRPGFKGRHCELACEKVPRPCTRLFSETKSFPVWEGDVCHHVYKKVYKVHQDVCFKERCQSTSLKKLKQESN

Target ORF information:

RefSeq Version	XM_008767373.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus sushi, nidogen and EGF-like domains 1 (Sned1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008767373.2

ORF Insert Sequence:

ATGCGCCGTG GCGCCGCCTG GGCGCTGCTG CTGGCCGCAG CCCTGGGGCT CGGGGCGCGC 
GGGGTGCGCG CTGCGGTGGC CCTCGCCGAC TTCTACCCGT TCGGCACGAA GCGTGGCGAC 
GCCGTCACCC CGAAGCAGGA CGACGGCGGC TCAGGGCTGC AGCCCCTCTC GGTGCCCTTC 
CCGTTCTTCG GCGCCGAGCA CTCAGGACTC TACGTGAACA ACAACGGGAT CATCTCCTTC 
CTGAAGGAAG TTTCTCAGTT CACCCCTGTG GCCTTCCCCA TCGCCAAAGA CCGCTGTGTG 
GTAGCGGCCT TCTGGGCAGA CGTGGATAAC CGACGCGCAG GCGACGTCTA CTACCGGGAG 
GCCACCGACG CAGCCATGCT GAACAGAGCC ACGGAGGACA TCAGGCGGTA CTTTCCTGAG 
CTCCCGGACT TCTCTGCTAC CTGGGTTTTT GTTGCCACCT GGTACCGTGT GACCTTCTTC 
GGAGGCAGCA GCTCTTCCCC CGTCAACACA TTCCAAACGG TGCTCATCAC TGACGGCCGG 
TTCTCCTTCA CCATCTTCAA CTATGAGTCC ATCCTGTGGA CTACGGGCAC ACACGCCAGC 
AGTGGTGGTG ATGCTGATGG CTTGGGAGGC ATTGCAGCCC AGGCAGGTTT CAACGCAGGT 
GATGGGCACC GCTACTTCAA CATCCCTGGG TCGCGCACAG CAGACATGGC TGAGGTGGAG 
ACCACCACCA ACGTGGGCGT GCCCGGGCGC TGGGCGTTTA GAATCGATGA TGCCCAGGTG 
CGCGTGGGGG GCTGCGGCCA TACAACCTCT GTGTGCCTAG TCCTGCGTCC GTGCCTCAAT 
GGTGGCAAGT GCATCGATGA CTGTGTCACG GGCAATCCCT CCTACACCTG TTCCTGTCTC 
GCTGGCTTCA CGGGGCGTAG ATGCCACCTG GATGTGAACG AGTGTGCTTC CCACCCATGT 
CAGAACGGTG GGACCTGCAC CCACGGTGTC AACAGCTTCA GTTGCCAGTG CCCAGCCGGC 
TTCCAGGGAC CCACCTGTGA ATCAGCCCAG TCTCCGTGTG ACAACAAAGT GTGTCAAAAT 
GGTGGCCAGT GCCAGGCAGA GAGCAGCTCT GCAGTATGTG TGTGTCAAGC TGGATACACT 
GGGGCCACCT GTGAGACCGA TGTGGATGAA TGCAGCTCTG ACCCATGCCT AAATGGTGGA 
TCGTGTGTTG ACCTGGTTGG AAACTACAGC TGTATTTGTG TGGAGCCCTT TGAAGGACCT 
CAGTGCGAGA CAGGAAGCTA CGTGGTGCCT TCGCCCTGCC TCTCCAACCC CTGCCTGAAC 
GGGGGCACCT GTGTGGATGC TGACCAGGGA TACGTGTGCG AATGCCCTGA AGGTTTCATG 
GGCTTGGACT GCAGAGAGAG AATTCTCAAT GACTGCGATT GCCGGAATGG AGGCAGATGC 
CTGGGTGCCA ACACCACCAT CTGCCAGTGT CCTCCAGGCT TCTTTGGGCT CCTCTGTGAA 
TTTGAAGTCA CAGCCACGCC CTGCAACATG AACACTCAGT GTCCAGATGG AGGCTACTGC 
ATGGAGTATG GCGGAAGCTA CCTATGTGTC TGCCACACGG ACCACAACAT CAGCCATTCT 
CTGCCCTCGC CCTGCGACTC AGACCCATGC TTTAATGGAG GTTCCTGTGA CGCCCACGAG 
GACTCCTACA CGTGCGAGTG CCCTCGTGGA TTCCATGGCA GGCACTGCGA GAAAGCCCGG 
CCACACCTGT GCAGCTCAGG GCCCTGCCGG AATGGGGGCA CATGCAAGGA GACTGGTGAC 
GAGTACCGCT GCACCTGCCC TTACCGGTTC ACTGGGAGAC ACTGTGAGAT TGGAAAGCCA 
GACTCCTGTG CCTCTGGCCC CTGTCACAAC GGTGGCACTT GTTTCCACTA CATTGGCAAA 
TACAAGTGTG ACTGCCCTCC AGGCTTCTCT GGTCGGCACT GTGAGATAGC CCCCTCCCCC 
TGCTTCCGGA GCCCATGTAT GAATGGGGGT ATCTGCGAGG ATCTAGGAAC AGATTTCTCC 
TGCCACTGCC AACCAGGATA TACAGGACAC CGGTGTCAGG CAGAGGTGGA CTGCGGTCAG 
CCTGAGGAGG TAAAACATGC TACCATGCGT CTCAATGGAA CTCGCATGGG CTCGGTGGCC 
CTGTACACAT GTGACCCCGG CTTCAGCCTG AGCGTCCTCA GCCATATGCG TGTCTGTCAG 
CCACAAGGTG TCTGGAGCCA GCCTCCCCAG TGCATTGAAG TAGATGAGTG CCAGTCTCAG 
CCATGCCTGC ATAAAGGCTC CTGCCAGGAC CTCATTGCTG GTTACCAGTG CCTCTGCAGC 
CCGGGGTACG AAGGAGTCCA CTGTGAGCTA GAGACAGACG AGTGCCAAGC ACAGCCCTGC 
AGAAATGGAG GCTCCTGCAG GGACCTCCCC GGGGCTTTCA TCTGCCAGTG CCCTGAAGGT 
TTTGTTGGAA CCCACTGTGA AACAGGTAGG GTTCTTTCAG GAGGGTCCCA CAGAACCAGG 
GCTGCTGGGA GATCAGAGGG GAAGCCAGGA GCCATCCTCT TGCTGGACAA GCCACCCAGA 
GAGAGAAACC CCCAAGGCTG TGGTGACCTG GAGGGAGGAA GGAAACTGGG CGGGGGGTCC 
CTGAGAGCCC CTCCTACCCA CAGGTGTGCC ATACCCCTCT CCTGGCCATT GCTCCTTGCC 
TCCCCCTATC CCGTCTTCCC GCTGTCCTCT CCTCCCTCCC CCAGAGTCCC TCCCTGCAGC 
TCGGGCCAAC CCTTTGGGTG TTCTCCAGAG GTGGATGCCT GTGCCTCCAG CCCCTGCCAG 
CACGGAGGCC GGTGTGAGGA CGGTGGTGGG GCCTACCTGT GCGTGTGTCC AGAGGGCTTC 
TTCGGCTACA ACTGTGAGAC AGTGAGTGAC CCCTGCTTCT CTAGCCCCTG TGGGGGCCGC 
GGCTACTGCT TGGCCAGCAA CGGGTCCCAC AGCTGTACCT GCAAAGTGGG CTACACAGGC 
AAGGACTGCA CCAAAGAGCT CCTCCCACCA ACAGCCCTCA GGGTAGAAAG GGTGGAGGAG 
AGTGGGGTCT CCATCTCCTG GAGCCCACCC GAGGGCACCA CGGCCAGACA GGTGCTGGAC 
GGCTATGCAG TCACCTATGC CTCCTCGGAT GGATCGTCCA GGCGCACGGA CTTTGTGGAC 
CGGAGCCGCT CCTCTCACCA GCTTCGGGCC CTGGCAGCCG GCCGTGCCTA CAACATCTCT 
GTTTTCTCAG TCAAGAGAAA CACTAACAAC AAAAATGACA TCAGCAGGCC TGCAGCCCTG 
CTCACCCGCA CCCGACCCCG CCCTATTGAA GACTTCGAGG TCACCAACAT TTCAGCCAAT 
GCCATCTCAG TGCAGTGGGC TCTTCATAGG ATCCAGCATG CCACTGTCAG CAGGGTTCGA 
GTGTCTGTCC TCTACCCTGA GGACACTGTG GTCCAGTCCA CGGAGGTGGA CAGGAGTGTG 
GACCGCCTCA CATTTGGGGA CCTGCTGCCA GGGAGAAGAT ACAGTGTGCG GCTAACCACC 
CTCAGTGGGC CTGGAGGAGC TGAATATCCT ACAGAGAGCC TGGCCTCAGC TCCGCTGAAC 
GTGTGGACCC GGCCTTTGCC TCCAGCAAAC CTGACTGCCT CTCGAGTCAC AGCGACCTCT 
GCCCATATGG TCTGGGACCC GCCCACTCCA GGCATCTCAC TGGAGGCTTA CGTCATCAAT 
GTGACCACCA GTCAGAATAC CAAGAGCCGC TACATCCCCA ATGGGAAGCT GGTGTCCTAT 
ACGGTGCGTG ATCTGATGCC AGGTCGGCGG TACCAGCTCT CGGTCACAGC GGTGCAGAGC 
ACAGAGCAGG GCCAGCTGCA CAGTGAGCCT GCGCACCTCT ACATCATCAC CTCCCCCAGG 
GATGGCACCG ACAGGCGCTG GCACCAGGGA GGACACCACT CACGAATGCT CAGAAATAGG 
CCGGCCCCTT TGCGCCTGCC AGAACTGCGC CTCCTCAATG ACCACGGTGC CCCTGAAACA 
CCAACCCAGC CACCCAGGTT CTCAGAGCTT GTAGACGGAA GAGCAAGAGT GAGTGCCAGG 
TTTGGTGGAT TGCCCAGCAG AGCAGTAACT GTGAGATCAC AACCCACTAC TCCGGTGCCG 
CTCAAGAACA CAGAGGCCCC TGAGCAGGCC CGTCTGGCCC TTCAGCTACC CAAGAACAAC 
AGCAAGGACA CAGAAAGTAC CCCTGGCAGC TGTTCAGAAG ACACCTGTCA GAATGGAGGC 
ACCTGTGTCC CAGGTGCCAA TGCCCACAGC TGTGACTGCA GGCCTGGGTT CAAAGGCAGA 
CACTGTGAGC TTGCCTGTGA AAAAGTGCCC CGCCCCTGCA CACGGCTGTT CTCTGAGACC 
AAGTCATTTC CTGTCTGGGA AGGAGATGTC TGCCACCATG TGTATAAGAA AGTCTACAAA 
GTTCACCAGG ACGTGTGTTT TAAGGAGCGC TGCCAGAGCA CAAGCCTCAA AAAGCTCAAA 
CAGGAATCAA ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sned1 ( XM_008767374.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008765596.1 Rattus norvegicus sushi, nidogen and EGF-like domains 1 (Sned1), transcript variant X2, mRNA.

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CloneID	ORa47569
Clone ID Related Accession (Same CDS sequence)	XM_008767374.2
Accession Version	XM_008767374.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4227bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	sushi, nidogen and EGF-like domain-containing protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005108.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008767374.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGCCGTG GCGCCGCCTG GGCGCTGCTG CTGGCCGCAG CCCTGGGGCT CGGGGCGCGC 
GGGGTGCGCG CTGCGGTGGC CCTCGCCGAC TTCTACCCGT TCGGCACGAA GCGTGGCGAC 
GCCGTCACCC CGAAGCAGGA CGACGGCGGC TCAGGGCTGC AGCCCCTCTC GGTGCCCTTC 
CCGTTCTTCG GCGCCGAGCA CTCAGGACTC TACGTGAACA ACAACGGGAT CATCTCCTTC 
CTGAAGGAAG TTTCTCAGTT CACCCCTGTG GCCTTCCCCA TCGCCAAAGA CCGCTGTGTG 
GTAGCGGCCT TCTGGGCAGA CGTGGATAAC CGACGCGCAG GCGACGTCTA CTACCGGGAG 
GCCACCGACG CAGCCATGCT GAACAGAGCC ACGGAGGACA TCAGGCGGTA CTTTCCTGAG 
CTCCCGGACT TCTCTGCTAC CTGGGTTTTT GTTGCCACCT GGTACCGTGT GACCTTCTTC 
GGAGGCAGCA GCTCTTCCCC CGTCAACACA TTCCAAACGG TGCTCATCAC TGACGGCCGG 
TTCTCCTTCA CCATCTTCAA CTATGAGTCC ATCCTGTGGA CTACGGGCAC ACACGCCAGC 
AGTGGTGGTG ATGCTGATGG CTTGGGAGGC ATTGCAGCCC AGGCAGGTTT CAACGCAGGT 
GATGGGCACC GCTACTTCAA CATCCCTGGG TCGCGCACAG CAGACATGGC TGAGGTGGAG 
ACCACCACCA ACGTGGGCGT GCCCGGGCGC TGGGCGTTTA GAATCGATGA TGCCCAGGTG 
CGCGTGGGGG GCTGCGGCCA TACAACCTCT GTGTGCCTAG TCCTGCGTCC GTGCCTCAAT 
GGTGGCAAGT GCATCGATGA CTGTGTCACG GGCAATCCCT CCTACACCTG TTCCTGTCTC 
GCTGGCTTCA CGGGGCGTAG ATGCCACCTG GATGTGAACG AGTGTGCTTC CCACCCATGT 
CAGAACGGTG GGACCTGCAC CCACGGTGTC AACAGCTTCA GTTGCCAGTG CCCAGCCGGC 
TTCCAGGGAC CCACCTGTGA ATCAGCCCAG TCTCCGTGTG ACAACAAAGT GTGTCAAAAT 
GGTGGCCAGT GCCAGGCAGA GAGCAGCTCT GCAGTATGTG TGTGTCAAGC TGGATACACT 
GGGGCCACCT GTGAGACCGA TGTGGATGAA TGCAGCTCTG ACCCATGCCT AAATGGTGGA 
TCGTGTGTTG ACCTGGTTGG AAACTACAGC TGTATTTGTG TGGAGCCCTT TGAAGGACCT 
CAGTGCGAGA CAGGAAGCTA CGTGGTGCCT TCGCCCTGCC TCTCCAACCC CTGCCTGAAC 
GGGGGCACCT GTGTGGATGC TGACCAGGGA TACGTGTGCG AATGCCCTGA AGGTTTCATG 
GGCTTGGACT GCAGAGAGAG AATTCTCAAT GACTGCGATT GCCGGAATGG AGGCAGATGC 
CTGGGTGCCA ACACCACCAT CTGCCAGTGT CCTCCAGGCT TCTTTGGGCT CCTCTGTGAA 
TTTGAAGTCA CAGCCACGCC CTGCAACATG AACACTCAGT GTCCAGATGG AGGCTACTGC 
ATGGAGTATG GCGGAAGCTA CCTATGTGTC TGCCACACGG ACCACAACAT CAGCCATTCT 
CTGCCCTCGC CCTGCGACTC AGACCCATGC TTTAATGGAG GTTCCTGTGA CGCCCACGAG 
GACTCCTACA CGTGCGAGTG CCCTCGTGGA TTCCATGGCA GGCACTGCGA GAAAGCCCGG 
CCACACCTGT GCAGCTCAGG GCCCTGCCGG AATGGGGGCA CATGCAAGGA GACTGGTGAC 
GAGTACCGCT GCACCTGCCC TTACCGGTTC ACTGGGAGAC ACTGTGAGAT TGGAAAGCCA 
GACTCCTGTG CCTCTGGCCC CTGTCACAAC GGTGGCACTT GTTTCCACTA CATTGGCAAA 
TACAAGTGTG ACTGCCCTCC AGGCTTCTCT GGTCGGCACT GTGAGATAGC CCCCTCCCCC 
TGCTTCCGGA GCCCATGTAT GAATGGGGGT ATCTGCGAGG ATCTAGGAAC AGATTTCTCC 
TGCCACTGCC AACCAGGATA TACAGGACAC CGGTGTCAGG CAGAGGTGGA CTGCGGTCAG 
CCTGAGGAGG TAAAACATGC TACCATGCGT CTCAATGGAA CTCGCATGGG CTCGGTGGCC 
CTGTACACAT GTGACCCCGG CTTCAGCCTG AGCGTCCTCA GCCATATGCG TGTCTGTCAG 
CCACAAGGTG TCTGGAGCCA GCCTCCCCAG TGCATTGAAG TAGATGAGTG CCAGTCTCAG 
CCATGCCTGC ATAAAGGCTC CTGCCAGGAC CTCATTGCTG GTTACCAGTG CCTCTGCAGC 
CCGGGGTACG AAGGAGTCCA CTGTGAGCTA GAGACAGACG AGTGCCAAGC ACAGCCCTGC 
AGAAATGGAG GCTCCTGCAG GGACCTCCCC GGGGCTTTCA TCTGCCAGTG CCCTGAAGGT 
TTTGTTGGAA CCCACTGTGA AACAGGTAGG GTTCTTTCAG GAGGGTCCCA CAGAACCAGG 
GCTGCTGGGA GATCAGAGGG GAAGCCAGGA GCCATCCTCT TGCTGGACAA GCCACCCAGA 
GAGAGAAACC CCCAAGGCTG TGGTGACCTG GAGGGAGGAA GGAAACTGGG CGGGGGGTCC 
CTGAGAGCCC CTCCTACCCA CAGGTGTGCC ATACCCCTCT CCTGGCCATT GCTCCTTGCC 
TCCCCCTATC CCGTCTTCCC GCTGTCCTCT CCTCCCTCCC CCAGAGTCCC TCCCTGCAGC 
TCGGGCCAAC CCTTTGGGTG TTCTCCAGAG GTGGATGCCT GTGCCTCCAG CCCCTGCCAG 
CACGGAGGCC GGTGTGAGGA CGGTGGTGGG GCCTACCTGT GCGTGTGTCC AGAGGGCTTC 
TTCGGCTACA ACTGTGAGAC AGTGAGTGAC CCCTGCTTCT CTAGCCCCTG TGGGGGCCGC 
GGCTACTGCT TGGCCAGCAA CGGGTCCCAC AGCTGTACCT GCAAAGTGGG CTACACAGGC 
AAGGACTGCA CCAAAGAGCT CCTCCCACCA ACAGCCCTCA GGGTAGAAAG GGTGGAGGAG 
AGTGGGGTCT CCATCTCCTG GAGCCCACCC GAGGGCACCA CGGCCAGACA GGTGCTGGAC 
GGCTATGCAG TCACCTATGC CTCCTCGGAT GGATCGTCCA GGCGCACGGA CTTTGTGGAC 
CGGAGCCGCT CCTCTCACCA GCTTCGGGCC CTGGCAGCCG GCCGTGCCTA CAACATCTCT 
GTTTTCTCAG TCAAGAGAAA CACTAACAAC AAAAATGACA TCAGCAGGCC TGCAGCCCTG 
CTCACCCGCA CCCGACCCCG CCCTATTGAA GACTTCGAGG TCACCAACAT TTCAGCCAAT 
GCCATCTCAG TGCAGTGGGC TCTTCATAGG ATCCAGCATG CCACTGTCAG CAGGGTTCGA 
GTGTCTGTCC TCTACCCTGA GGACACTGTG GTCCAGTCCA CGGAGGTGGA CAGGAGTGTG 
GACCGCCTCA CATTTGGGGA CCTGCTGCCA GGGAGAAGAT ACAGTGTGCG GCTAACCACC 
CTCAGTGGGC CTGGAGGAGC TGAATATCCT ACAGAGAGCC TGGCCTCAGC TCCGCTGAAC 
GTGTGGACCC GGCCTTTGCC TCCAGCAAAC CTGACTGCCT CTCGAGTCAC AGCGACCTCT 
GCCCATATGG TCTGGGACCC GCCCACTCCA GGCATCTCAC TGGAGGCTTA CGTCATCAAT 
GTGACCACCA GTCAGAATAC CAAGAGCCGC TACATCCCCA ATGGGAAGCT GGTGTCCTAT 
ACGGTGCGTG ATCTGATGCC AGGTCGGCGG TACCAGCTCT CGGTCACAGC GGTGCAGAGC 
ACAGAGCAGG GCCAGCTGCA CAGTGAGCCT GCGCACCTCT ACATCATCAC CTCCCCCAGG 
GATGGCACCG ACAGGCGCTG GCACCAGGGA GGACACCACT CACGAATGCT CAGAAATAGG 
CCGGCCCCTT TGCGCCTGCC AGAACTGCGC CTCCTCAATG ACCACGGTGC CCCTGAAACA 
CCAACCCAGC CACCCAGGTT CTCAGAGCTT GTAGACGGAA GAGCAAGAGT GAGTGCCAGG 
TTTGGTGGAT TGCCCAGCAG AGCAGTAACT GTGAGATCAC AACCCACTAC TCCGGTGCCG 
CTCAAGAACA CAGAGGCCCC TGAGCAGGCC CGTCTGGCCC TTCAGCTACC CAAGAACAAC 
AGCAAGGACA CAGAAAGGCT GCTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008765596.1
CDS	180..4406
Translation	MRRGAAWALLLAAALGLGARGVRAAVALADFYPFGTKRGDAVTPKQDDGGSGLQPLSVPFPFFGAEHSGLYVNNNGIISFLKEVSQFTPVAFPIAKDRCVVAAFWADVDNRRAGDVYYREATDAAMLNRATEDIRRYFPELPDFSATWVFVATWYRVTFFGGSSSSPVNTFQTVLITDGRFSFTIFNYESILWTTGTHASSGGDADGLGGIAAQAGFNAGDGHRYFNIPGSRTADMAEVETTTNVGVPGRWAFRIDDAQVRVGGCGHTTSVCLVLRPCLNGGKCIDDCVTGNPSYTCSCLAGFTGRRCHLDVNECASHPCQNGGTCTHGVNSFSCQCPAGFQGPTCESAQSPCDNKVCQNGGQCQAESSSAVCVCQAGYTGATCETDVDECSSDPCLNGGSCVDLVGNYSCICVEPFEGPQCETGSYVVPSPCLSNPCLNGGTCVDADQGYVCECPEGFMGLDCRERILNDCDCRNGGRCLGANTTICQCPPGFFGLLCEFEVTATPCNMNTQCPDGGYCMEYGGSYLCVCHTDHNISHSLPSPCDSDPCFNGGSCDAHEDSYTCECPRGFHGRHCEKARPHLCSSGPCRNGGTCKETGDEYRCTCPYRFTGRHCEIGKPDSCASGPCHNGGTCFHYIGKYKCDCPPGFSGRHCEIAPSPCFRSPCMNGGICEDLGTDFSCHCQPGYTGHRCQAEVDCGQPEEVKHATMRLNGTRMGSVALYTCDPGFSLSVLSHMRVCQPQGVWSQPPQCIEVDECQSQPCLHKGSCQDLIAGYQCLCSPGYEGVHCELETDECQAQPCRNGGSCRDLPGAFICQCPEGFVGTHCETGRVLSGGSHRTRAAGRSEGKPGAILLLDKPPRERNPQGCGDLEGGRKLGGGSLRAPPTHRCAIPLSWPLLLASPYPVFPLSSPPSPRVPPCSSGQPFGCSPEVDACASSPCQHGGRCEDGGGAYLCVCPEGFFGYNCETVSDPCFSSPCGGRGYCLASNGSHSCTCKVGYTGKDCTKELLPPTALRVERVEESGVSISWSPPEGTTARQVLDGYAVTYASSDGSSRRTDFVDRSRSSHQLRALAAGRAYNISVFSVKRNTNNKNDISRPAALLTRTRPRPIEDFEVTNISANAISVQWALHRIQHATVSRVRVSVLYPEDTVVQSTEVDRSVDRLTFGDLLPGRRYSVRLTTLSGPGGAEYPTESLASAPLNVWTRPLPPANLTASRVTATSAHMVWDPPTPGISLEAYVINVTTSQNTKSRYIPNGKLVSYTVRDLMPGRRYQLSVTAVQSTEQGQLHSEPAHLYIITSPRDGTDRRWHQGGHHSRMLRNRPAPLRLPELRLLNDHGAPETPTQPPRFSELVDGRARVSARFGGLPSRAVTVRSQPTTPVPLKNTEAPEQARLALQLPKNNSKDTERLL

Target ORF information:

RefSeq Version	XM_008767374.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus sushi, nidogen and EGF-like domains 1 (Sned1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008767374.2

ORF Insert Sequence:

ATGCGCCGTG GCGCCGCCTG GGCGCTGCTG CTGGCCGCAG CCCTGGGGCT CGGGGCGCGC 
GGGGTGCGCG CTGCGGTGGC CCTCGCCGAC TTCTACCCGT TCGGCACGAA GCGTGGCGAC 
GCCGTCACCC CGAAGCAGGA CGACGGCGGC TCAGGGCTGC AGCCCCTCTC GGTGCCCTTC 
CCGTTCTTCG GCGCCGAGCA CTCAGGACTC TACGTGAACA ACAACGGGAT CATCTCCTTC 
CTGAAGGAAG TTTCTCAGTT CACCCCTGTG GCCTTCCCCA TCGCCAAAGA CCGCTGTGTG 
GTAGCGGCCT TCTGGGCAGA CGTGGATAAC CGACGCGCAG GCGACGTCTA CTACCGGGAG 
GCCACCGACG CAGCCATGCT GAACAGAGCC ACGGAGGACA TCAGGCGGTA CTTTCCTGAG 
CTCCCGGACT TCTCTGCTAC CTGGGTTTTT GTTGCCACCT GGTACCGTGT GACCTTCTTC 
GGAGGCAGCA GCTCTTCCCC CGTCAACACA TTCCAAACGG TGCTCATCAC TGACGGCCGG 
TTCTCCTTCA CCATCTTCAA CTATGAGTCC ATCCTGTGGA CTACGGGCAC ACACGCCAGC 
AGTGGTGGTG ATGCTGATGG CTTGGGAGGC ATTGCAGCCC AGGCAGGTTT CAACGCAGGT 
GATGGGCACC GCTACTTCAA CATCCCTGGG TCGCGCACAG CAGACATGGC TGAGGTGGAG 
ACCACCACCA ACGTGGGCGT GCCCGGGCGC TGGGCGTTTA GAATCGATGA TGCCCAGGTG 
CGCGTGGGGG GCTGCGGCCA TACAACCTCT GTGTGCCTAG TCCTGCGTCC GTGCCTCAAT 
GGTGGCAAGT GCATCGATGA CTGTGTCACG GGCAATCCCT CCTACACCTG TTCCTGTCTC 
GCTGGCTTCA CGGGGCGTAG ATGCCACCTG GATGTGAACG AGTGTGCTTC CCACCCATGT 
CAGAACGGTG GGACCTGCAC CCACGGTGTC AACAGCTTCA GTTGCCAGTG CCCAGCCGGC 
TTCCAGGGAC CCACCTGTGA ATCAGCCCAG TCTCCGTGTG ACAACAAAGT GTGTCAAAAT 
GGTGGCCAGT GCCAGGCAGA GAGCAGCTCT GCAGTATGTG TGTGTCAAGC TGGATACACT 
GGGGCCACCT GTGAGACCGA TGTGGATGAA TGCAGCTCTG ACCCATGCCT AAATGGTGGA 
TCGTGTGTTG ACCTGGTTGG AAACTACAGC TGTATTTGTG TGGAGCCCTT TGAAGGACCT 
CAGTGCGAGA CAGGAAGCTA CGTGGTGCCT TCGCCCTGCC TCTCCAACCC CTGCCTGAAC 
GGGGGCACCT GTGTGGATGC TGACCAGGGA TACGTGTGCG AATGCCCTGA AGGTTTCATG 
GGCTTGGACT GCAGAGAGAG AATTCTCAAT GACTGCGATT GCCGGAATGG AGGCAGATGC 
CTGGGTGCCA ACACCACCAT CTGCCAGTGT CCTCCAGGCT TCTTTGGGCT CCTCTGTGAA 
TTTGAAGTCA CAGCCACGCC CTGCAACATG AACACTCAGT GTCCAGATGG AGGCTACTGC 
ATGGAGTATG GCGGAAGCTA CCTATGTGTC TGCCACACGG ACCACAACAT CAGCCATTCT 
CTGCCCTCGC CCTGCGACTC AGACCCATGC TTTAATGGAG GTTCCTGTGA CGCCCACGAG 
GACTCCTACA CGTGCGAGTG CCCTCGTGGA TTCCATGGCA GGCACTGCGA GAAAGCCCGG 
CCACACCTGT GCAGCTCAGG GCCCTGCCGG AATGGGGGCA CATGCAAGGA GACTGGTGAC 
GAGTACCGCT GCACCTGCCC TTACCGGTTC ACTGGGAGAC ACTGTGAGAT TGGAAAGCCA 
GACTCCTGTG CCTCTGGCCC CTGTCACAAC GGTGGCACTT GTTTCCACTA CATTGGCAAA 
TACAAGTGTG ACTGCCCTCC AGGCTTCTCT GGTCGGCACT GTGAGATAGC CCCCTCCCCC 
TGCTTCCGGA GCCCATGTAT GAATGGGGGT ATCTGCGAGG ATCTAGGAAC AGATTTCTCC 
TGCCACTGCC AACCAGGATA TACAGGACAC CGGTGTCAGG CAGAGGTGGA CTGCGGTCAG 
CCTGAGGAGG TAAAACATGC TACCATGCGT CTCAATGGAA CTCGCATGGG CTCGGTGGCC 
CTGTACACAT GTGACCCCGG CTTCAGCCTG AGCGTCCTCA GCCATATGCG TGTCTGTCAG 
CCACAAGGTG TCTGGAGCCA GCCTCCCCAG TGCATTGAAG TAGATGAGTG CCAGTCTCAG 
CCATGCCTGC ATAAAGGCTC CTGCCAGGAC CTCATTGCTG GTTACCAGTG CCTCTGCAGC 
CCGGGGTACG AAGGAGTCCA CTGTGAGCTA GAGACAGACG AGTGCCAAGC ACAGCCCTGC 
AGAAATGGAG GCTCCTGCAG GGACCTCCCC GGGGCTTTCA TCTGCCAGTG CCCTGAAGGT 
TTTGTTGGAA CCCACTGTGA AACAGGTAGG GTTCTTTCAG GAGGGTCCCA CAGAACCAGG 
GCTGCTGGGA GATCAGAGGG GAAGCCAGGA GCCATCCTCT TGCTGGACAA GCCACCCAGA 
GAGAGAAACC CCCAAGGCTG TGGTGACCTG GAGGGAGGAA GGAAACTGGG CGGGGGGTCC 
CTGAGAGCCC CTCCTACCCA CAGGTGTGCC ATACCCCTCT CCTGGCCATT GCTCCTTGCC 
TCCCCCTATC CCGTCTTCCC GCTGTCCTCT CCTCCCTCCC CCAGAGTCCC TCCCTGCAGC 
TCGGGCCAAC CCTTTGGGTG TTCTCCAGAG GTGGATGCCT GTGCCTCCAG CCCCTGCCAG 
CACGGAGGCC GGTGTGAGGA CGGTGGTGGG GCCTACCTGT GCGTGTGTCC AGAGGGCTTC 
TTCGGCTACA ACTGTGAGAC AGTGAGTGAC CCCTGCTTCT CTAGCCCCTG TGGGGGCCGC 
GGCTACTGCT TGGCCAGCAA CGGGTCCCAC AGCTGTACCT GCAAAGTGGG CTACACAGGC 
AAGGACTGCA CCAAAGAGCT CCTCCCACCA ACAGCCCTCA GGGTAGAAAG GGTGGAGGAG 
AGTGGGGTCT CCATCTCCTG GAGCCCACCC GAGGGCACCA CGGCCAGACA GGTGCTGGAC 
GGCTATGCAG TCACCTATGC CTCCTCGGAT GGATCGTCCA GGCGCACGGA CTTTGTGGAC 
CGGAGCCGCT CCTCTCACCA GCTTCGGGCC CTGGCAGCCG GCCGTGCCTA CAACATCTCT 
GTTTTCTCAG TCAAGAGAAA CACTAACAAC AAAAATGACA TCAGCAGGCC TGCAGCCCTG 
CTCACCCGCA CCCGACCCCG CCCTATTGAA GACTTCGAGG TCACCAACAT TTCAGCCAAT 
GCCATCTCAG TGCAGTGGGC TCTTCATAGG ATCCAGCATG CCACTGTCAG CAGGGTTCGA 
GTGTCTGTCC TCTACCCTGA GGACACTGTG GTCCAGTCCA CGGAGGTGGA CAGGAGTGTG 
GACCGCCTCA CATTTGGGGA CCTGCTGCCA GGGAGAAGAT ACAGTGTGCG GCTAACCACC 
CTCAGTGGGC CTGGAGGAGC TGAATATCCT ACAGAGAGCC TGGCCTCAGC TCCGCTGAAC 
GTGTGGACCC GGCCTTTGCC TCCAGCAAAC CTGACTGCCT CTCGAGTCAC AGCGACCTCT 
GCCCATATGG TCTGGGACCC GCCCACTCCA GGCATCTCAC TGGAGGCTTA CGTCATCAAT 
GTGACCACCA GTCAGAATAC CAAGAGCCGC TACATCCCCA ATGGGAAGCT GGTGTCCTAT 
ACGGTGCGTG ATCTGATGCC AGGTCGGCGG TACCAGCTCT CGGTCACAGC GGTGCAGAGC 
ACAGAGCAGG GCCAGCTGCA CAGTGAGCCT GCGCACCTCT ACATCATCAC CTCCCCCAGG 
GATGGCACCG ACAGGCGCTG GCACCAGGGA GGACACCACT CACGAATGCT CAGAAATAGG 
CCGGCCCCTT TGCGCCTGCC AGAACTGCGC CTCCTCAATG ACCACGGTGC CCCTGAAACA 
CCAACCCAGC CACCCAGGTT CTCAGAGCTT GTAGACGGAA GAGCAAGAGT GAGTGCCAGG 
TTTGGTGGAT TGCCCAGCAG AGCAGTAACT GTGAGATCAC AACCCACTAC TCCGGTGCCG 
CTCAAGAACA CAGAGGCCCC TGAGCAGGCC CGTCTGGCCC TTCAGCTACC CAAGAACAAC 
AGCAAGGACA CAGAAAGGCT GCTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sned1 ( XM_017596495.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017451984.1 Rattus norvegicus sushi, nidogen and EGF-like domains 1 (Sned1), transcript variant X5, mRNA.

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CloneID	ORa62788
Clone ID Related Accession (Same CDS sequence)	XM_017596495.1
Accession Version	XM_017596495.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4098bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	sushi, nidogen and EGF-like domain-containing protein 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005108.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGCCGTG GCGCCGCCTG GGCGCTGCTG CTGGCCGCAG CCCTGGGGCT CGGGGCGCGC 
GGGGTGCGCG CTGCGGTGGC CCTCGCCGAC TTCTACCCGT TCGGCACGAA GCGTGGCGAC 
GCCGTCACCC CGAAGCAGGA CGACGGCGGC TCAGGGCTGC AGCCCCTCTC GGTGCCCTTC 
CCGTTCTTCG GCGCCGAGCA CTCAGGACTC TACGTGAACA ACAACGGGAT CATCTCCTTC 
CTGAAGGAAG TTTCTCAGTT CACCCCTGTG GCCTTCCCCA TCGCCAAAGA CCGCTGTGTG 
GTAGCGGCCT TCTGGGCAGA CGTGGATAAC CGACGCGCAG GCGACGTCTA CTACCGGGAG 
GCCACCGACG CAGCCATGCT GAACAGAGCC ACGGAGGACA TCAGGCGGTA CTTTCCTGAG 
CTCCCGGACT TCTCTGCTAC CTGGGTTTTT GTTGCCACCT GGTACCGTGT GACCTTCTTC 
GGAGGCAGCA GCTCTTCCCC CGTCAACACA TTCCAAACGG TGCTCATCAC TGACGGCCGG 
TTCTCCTTCA CCATCTTCAA CTATGAGTCC ATCCTGTGGA CTACGGGCAC ACACGCCAGC 
AGTGGTGGTG ATGCTGATGG CTTGGGAGGC ATTGCAGCCC AGGCAGGTTT CAACGCAGGT 
GATGGGCACC GCTACTTCAA CATCCCTGGG TCGCGCACAG CAGACATGGC TGAGGTGGAG 
ACCACCACCA ACGTGGGCGT GCCCGGGCGC TGGGCGTTTA GAATCGATGA TGCCCAGGTG 
CGCGTGGGGG GCTGCGGCCA TACAACCTCT GTGTGCCTAG TCCTGCGTCC GTGCCTCAAT 
GGTGGCAAGT GCATCGATGA CTGTGTCACG GGCAATCCCT CCTACACCTG TTCCTGTCTC 
GCTGGCTTCA CGGGGCGTAG ATGCCACCTG GATGTGAACG AGTGTGCTTC CCACCCATGT 
CAGAACGGTG GGACCTGCAC CCACGGTGTC AACAGCTTCA GTTGCCAGTG CCCAGCCGGC 
TTCCAGGGAC CCACCTGTGA ATCAGCCCAG TCTCCGTGTG ACAACAAAGT GTGTCAAAAT 
GGTGGCCAGT GCCAGGCAGA GAGCAGCTCT GCAGTATGTG TGTGTCAAGC TGGATACACT 
GGGGCCACCT GTGAGACCGA TGTGGATGAA TGCAGCTCTG ACCCATGCCT AAATGGTGGA 
TCGTGTGTTG ACCTGGTTGG AAACTACAGC TGTATTTGTG TGGAGCCCTT TGAAGGACCT 
CAGTGCGAGA CAGGAAGCTA CGTGGTGCCT TCGCCCTGCC TCTCCAACCC CTGCCTGAAC 
GGGGGCACCT GTGTGGATGC TGACCAGGGA TACGTGTGCG AATGCCCTGA AGGTTTCATG 
GGCTTGGACT GCAGAGAGAG AATTCTCAAT GACTGCGATT GCCGGAATGG AGGCAGATGC 
CTGGGTGCCA ACACCACCAT CTGCCAGTGT CCTCCAGGCT TCTTTGGGCT CCTCTGTGAA 
TTTGAAGTCA CAGCCACGCC CTGCAACATG AACACTCAGT GTCCAGATGG AGGCTACTGC 
ATGGAGTATG GCGGAAGCTA CCTATGTGTC TGCCACACGG ACCACAACAT CAGCCATTCT 
CTGCCCTCGC CCTGCGACTC AGACCCATGC TTTAATGGAG GTTCCTGTGA CGCCCACGAG 
GACTCCTACA CGTGCGAGTG CCCTCGTGGA TTCCATGGCA GGCACTGCGA GAAAGCCCGG 
CCACACCTGT GCAGCTCAGG GCCCTGCCGG AATGGGGGCA CATGCAAGGA GACTGGTGAC 
GAGTACCGCT GCACCTGCCC TTACCGGTTC ACTGGGAGAC ACTGTGAGAT TGGAAAGCCA 
GACTCCTGTG CCTCTGGCCC CTGTCACAAC GGTGGCACTT GTTTCCACTA CATTGGCAAA 
TACAAGTGTG ACTGCCCTCC AGGCTTCTCT GGTCGGCACT GTGAGATAGC CCCCTCCCCC 
TGCTTCCGGA GCCCATGTAT GAATGGGGGT ATCTGCGAGG ATCTAGGAAC AGATTTCTCC 
TGCCACTGCC AACCAGGATA TACAGGACAC CGGTGTCAGG CAGAGGTGGA CTGCGGTCAG 
CCTGAGGAGG TAAAACATGC TACCATGCGT CTCAATGGAA CTCGCATGGG CTCGGTGGCC 
CTGTACACAT GTGACCCCGG CTTCAGCCTG AGCGTCCTCA GCCATATGCG TGTCTGTCAG 
CCACAAGGTG TCTGGAGCCA GCCTCCCCAG TGCATTGAAG TAGATGAGTG CCAGTCTCAG 
CCATGCCTGC ATAAAGGCTC CTGCCAGGAC CTCATTGCTG GTTACCAGTG CCTCTGCAGC 
CCGGGGTACG AAGGAGTCCA CTGTGAGCTA GAGGTGGATG CCTGTGCCTC CAGCCCCTGC 
CAGCACGGAG GCCGGTGTGA GGACGGTGGT GGGGCCTACC TGTGCGTGTG TCCAGAGGGC 
TTCTTCGGCT ACAACTGTGA GACAGTGAGT GACCCCTGCT TCTCTAGCCC CTGTGGGGGC 
CGCGGCTACT GCTTGGCCAG CAACGGGTCC CACAGCTGTA CCTGCAAAGT GGGCTACACA 
GGCAAGGACT GCACCAAAGA GCTCCTCCCA CCAACAGCCC TCAGGGTAGA AAGGGTGGAG 
GAGAGTGGGG TCTCCATCTC CTGGAGCCCA CCCGAGGGCA CCACGGCCAG ACAGGTGCTG 
GACGGCTATG CAGTCACCTA TGCCTCCTCG GATGGATCGT CCAGGCGCAC GGACTTTGTG 
GACCGGAGCC GCTCCTCTCA CCAGCTTCGG GCCCTGGCAG CCGGCCGTGC CTACAACATC 
TCTGTTTTCT CAGTCAAGAG AAACACTAAC AACAAAAATG ACATCAGCAG GCCTGCAGCC 
CTGCTCACCC GCACCCGACC CCGCCCTATT GAAGACTTCG AGGTCACCAA CATTTCAGCC 
AATGCCATCT CAGTGCAGTG GGCTCTTCAT AGGATCCAGC ATGCCACTGT CAGCAGGGTT 
CGAGTGTCTG TCCTCTACCC TGAGGACACT GTGGTCCAGT CCACGGAGGT GGACAGGAGT 
GTGGACCGCC TCACATTTGG GGACCTGCTG CCAGGGAGAA GATACAGTGT GCGGCTAACC 
ACCCTCAGTG GGCCTGGAGG AGCTGAATAT CCTACAGAGA GCCTGGCCTC AGCTCCGCTG 
AACGTGTGGA CCCGGCCTTT GCCTCCAGCA AACCTGACTG CCTCTCGAGT CACAGCGACC 
TCTGCCCATA TGGTCTGGGA CCCGCCCACT CCAGGCATCT CACTGGAGGC TTACGTCATC 
AATGTGACCA CCAGTCAGAA TACCAAGAGC CGCTACATCC CCAATGGGAA GCTGGTGTCC 
TATACGGTGC GTGATCTGAT GCCAGGTCGG CGGTACCAGC TCTCGGTCAC AGCGGTGCAG 
AGCACAGAGC AGGGCCAGCT GCACAGTGAG CCTGCGCACC TCTACATCAT CACCTCCCCC 
AGGGATGGCA CCGACAGGCG CTGGCACCAG GGAGGACACC ACTCACGAAT GCTCAGAAAT 
AGGCCGGCCC CTTTGCGCCT GCCAGAACTG CGCCTCCTCA ATGACCACGG TGCCCCTGAA 
ACACCAACCC AGCCACCCAG GTTCTCAGAG CTTGTAGACG GAAGAGCAAG AGTGAGTGCC 
AGGTTTGGTG GATTGCCCAG CAGAGCAGTA ACTGTGAGAT CACAACCCAC TACTCCGGTG 
CCGCTCAAGA ACACAGAGGC CCCTGAGCAG GCCCGTCTGG CCCTTCAGCT ACCCAAGAAC 
AACAGCAAGG ACACAGAAAG TACCCCTGGC AGCTGTTCAG AAGACACCTG TCAGAATGGA 
GGCACCTGTG TCCCAGGTGC CAATGCCCAC AGCTGTGACT GCAGGCCTGG GTTCAAAGGC 
AGACACTGTG AGCTTGCCTG TGAAAAAGTG CCCCGCCCCT GCACACGGCT GTTCTCTGAG 
ACCAAGTCAT TTCCTGTCTG GGAAGGAGAT GTCTGCCACC ATGTGTATAA GAAAGTCTAC 
AAAGTTCACC AGGACGTGTG TTTTAAGGAG CGCTGCCAGA GCACAAGCCT CAAAAAGCTC 
AAACAGGAAT CAAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017451984.1
CDS	178..4275
Translation	MRRGAAWALLLAAALGLGARGVRAAVALADFYPFGTKRGDAVTPKQDDGGSGLQPLSVPFPFFGAEHSGLYVNNNGIISFLKEVSQFTPVAFPIAKDRCVVAAFWADVDNRRAGDVYYREATDAAMLNRATEDIRRYFPELPDFSATWVFVATWYRVTFFGGSSSSPVNTFQTVLITDGRFSFTIFNYESILWTTGTHASSGGDADGLGGIAAQAGFNAGDGHRYFNIPGSRTADMAEVETTTNVGVPGRWAFRIDDAQVRVGGCGHTTSVCLVLRPCLNGGKCIDDCVTGNPSYTCSCLAGFTGRRCHLDVNECASHPCQNGGTCTHGVNSFSCQCPAGFQGPTCESAQSPCDNKVCQNGGQCQAESSSAVCVCQAGYTGATCETDVDECSSDPCLNGGSCVDLVGNYSCICVEPFEGPQCETGSYVVPSPCLSNPCLNGGTCVDADQGYVCECPEGFMGLDCRERILNDCDCRNGGRCLGANTTICQCPPGFFGLLCEFEVTATPCNMNTQCPDGGYCMEYGGSYLCVCHTDHNISHSLPSPCDSDPCFNGGSCDAHEDSYTCECPRGFHGRHCEKARPHLCSSGPCRNGGTCKETGDEYRCTCPYRFTGRHCEIGKPDSCASGPCHNGGTCFHYIGKYKCDCPPGFSGRHCEIAPSPCFRSPCMNGGICEDLGTDFSCHCQPGYTGHRCQAEVDCGQPEEVKHATMRLNGTRMGSVALYTCDPGFSLSVLSHMRVCQPQGVWSQPPQCIEVDECQSQPCLHKGSCQDLIAGYQCLCSPGYEGVHCELEVDACASSPCQHGGRCEDGGGAYLCVCPEGFFGYNCETVSDPCFSSPCGGRGYCLASNGSHSCTCKVGYTGKDCTKELLPPTALRVERVEESGVSISWSPPEGTTARQVLDGYAVTYASSDGSSRRTDFVDRSRSSHQLRALAAGRAYNISVFSVKRNTNNKNDISRPAALLTRTRPRPIEDFEVTNISANAISVQWALHRIQHATVSRVRVSVLYPEDTVVQSTEVDRSVDRLTFGDLLPGRRYSVRLTTLSGPGGAEYPTESLASAPLNVWTRPLPPANLTASRVTATSAHMVWDPPTPGISLEAYVINVTTSQNTKSRYIPNGKLVSYTVRDLMPGRRYQLSVTAVQSTEQGQLHSEPAHLYIITSPRDGTDRRWHQGGHHSRMLRNRPAPLRLPELRLLNDHGAPETPTQPPRFSELVDGRARVSARFGGLPSRAVTVRSQPTTPVPLKNTEAPEQARLALQLPKNNSKDTESTPGSCSEDTCQNGGTCVPGANAHSCDCRPGFKGRHCELACEKVPRPCTRLFSETKSFPVWEGDVCHHVYKKVYKVHQDVCFKERCQSTSLKKLKQESN

Target ORF information:

RefSeq Version	XM_017596495.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus sushi, nidogen and EGF-like domains 1 (Sned1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017596495.1

ORF Insert Sequence:

ATGCGCCGTG GCGCCGCCTG GGCGCTGCTG CTGGCCGCAG CCCTGGGGCT CGGGGCGCGC 
GGGGTGCGCG CTGCGGTGGC CCTCGCCGAC TTCTACCCGT TCGGCACGAA GCGTGGCGAC 
GCCGTCACCC CGAAGCAGGA CGACGGCGGC TCAGGGCTGC AGCCCCTCTC GGTGCCCTTC 
CCGTTCTTCG GCGCCGAGCA CTCAGGACTC TACGTGAACA ACAACGGGAT CATCTCCTTC 
CTGAAGGAAG TTTCTCAGTT CACCCCTGTG GCCTTCCCCA TCGCCAAAGA CCGCTGTGTG 
GTAGCGGCCT TCTGGGCAGA CGTGGATAAC CGACGCGCAG GCGACGTCTA CTACCGGGAG 
GCCACCGACG CAGCCATGCT GAACAGAGCC ACGGAGGACA TCAGGCGGTA CTTTCCTGAG 
CTCCCGGACT TCTCTGCTAC CTGGGTTTTT GTTGCCACCT GGTACCGTGT GACCTTCTTC 
GGAGGCAGCA GCTCTTCCCC CGTCAACACA TTCCAAACGG TGCTCATCAC TGACGGCCGG 
TTCTCCTTCA CCATCTTCAA CTATGAGTCC ATCCTGTGGA CTACGGGCAC ACACGCCAGC 
AGTGGTGGTG ATGCTGATGG CTTGGGAGGC ATTGCAGCCC AGGCAGGTTT CAACGCAGGT 
GATGGGCACC GCTACTTCAA CATCCCTGGG TCGCGCACAG CAGACATGGC TGAGGTGGAG 
ACCACCACCA ACGTGGGCGT GCCCGGGCGC TGGGCGTTTA GAATCGATGA TGCCCAGGTG 
CGCGTGGGGG GCTGCGGCCA TACAACCTCT GTGTGCCTAG TCCTGCGTCC GTGCCTCAAT 
GGTGGCAAGT GCATCGATGA CTGTGTCACG GGCAATCCCT CCTACACCTG TTCCTGTCTC 
GCTGGCTTCA CGGGGCGTAG ATGCCACCTG GATGTGAACG AGTGTGCTTC CCACCCATGT 
CAGAACGGTG GGACCTGCAC CCACGGTGTC AACAGCTTCA GTTGCCAGTG CCCAGCCGGC 
TTCCAGGGAC CCACCTGTGA ATCAGCCCAG TCTCCGTGTG ACAACAAAGT GTGTCAAAAT 
GGTGGCCAGT GCCAGGCAGA GAGCAGCTCT GCAGTATGTG TGTGTCAAGC TGGATACACT 
GGGGCCACCT GTGAGACCGA TGTGGATGAA TGCAGCTCTG ACCCATGCCT AAATGGTGGA 
TCGTGTGTTG ACCTGGTTGG AAACTACAGC TGTATTTGTG TGGAGCCCTT TGAAGGACCT 
CAGTGCGAGA CAGGAAGCTA CGTGGTGCCT TCGCCCTGCC TCTCCAACCC CTGCCTGAAC 
GGGGGCACCT GTGTGGATGC TGACCAGGGA TACGTGTGCG AATGCCCTGA AGGTTTCATG 
GGCTTGGACT GCAGAGAGAG AATTCTCAAT GACTGCGATT GCCGGAATGG AGGCAGATGC 
CTGGGTGCCA ACACCACCAT CTGCCAGTGT CCTCCAGGCT TCTTTGGGCT CCTCTGTGAA 
TTTGAAGTCA CAGCCACGCC CTGCAACATG AACACTCAGT GTCCAGATGG AGGCTACTGC 
ATGGAGTATG GCGGAAGCTA CCTATGTGTC TGCCACACGG ACCACAACAT CAGCCATTCT 
CTGCCCTCGC CCTGCGACTC AGACCCATGC TTTAATGGAG GTTCCTGTGA CGCCCACGAG 
GACTCCTACA CGTGCGAGTG CCCTCGTGGA TTCCATGGCA GGCACTGCGA GAAAGCCCGG 
CCACACCTGT GCAGCTCAGG GCCCTGCCGG AATGGGGGCA CATGCAAGGA GACTGGTGAC 
GAGTACCGCT GCACCTGCCC TTACCGGTTC ACTGGGAGAC ACTGTGAGAT TGGAAAGCCA 
GACTCCTGTG CCTCTGGCCC CTGTCACAAC GGTGGCACTT GTTTCCACTA CATTGGCAAA 
TACAAGTGTG ACTGCCCTCC AGGCTTCTCT GGTCGGCACT GTGAGATAGC CCCCTCCCCC 
TGCTTCCGGA GCCCATGTAT GAATGGGGGT ATCTGCGAGG ATCTAGGAAC AGATTTCTCC 
TGCCACTGCC AACCAGGATA TACAGGACAC CGGTGTCAGG CAGAGGTGGA CTGCGGTCAG 
CCTGAGGAGG TAAAACATGC TACCATGCGT CTCAATGGAA CTCGCATGGG CTCGGTGGCC 
CTGTACACAT GTGACCCCGG CTTCAGCCTG AGCGTCCTCA GCCATATGCG TGTCTGTCAG 
CCACAAGGTG TCTGGAGCCA GCCTCCCCAG TGCATTGAAG TAGATGAGTG CCAGTCTCAG 
CCATGCCTGC ATAAAGGCTC CTGCCAGGAC CTCATTGCTG GTTACCAGTG CCTCTGCAGC 
CCGGGGTACG AAGGAGTCCA CTGTGAGCTA GAGGTGGATG CCTGTGCCTC CAGCCCCTGC 
CAGCACGGAG GCCGGTGTGA GGACGGTGGT GGGGCCTACC TGTGCGTGTG TCCAGAGGGC 
TTCTTCGGCT ACAACTGTGA GACAGTGAGT GACCCCTGCT TCTCTAGCCC CTGTGGGGGC 
CGCGGCTACT GCTTGGCCAG CAACGGGTCC CACAGCTGTA CCTGCAAAGT GGGCTACACA 
GGCAAGGACT GCACCAAAGA GCTCCTCCCA CCAACAGCCC TCAGGGTAGA AAGGGTGGAG 
GAGAGTGGGG TCTCCATCTC CTGGAGCCCA CCCGAGGGCA CCACGGCCAG ACAGGTGCTG 
GACGGCTATG CAGTCACCTA TGCCTCCTCG GATGGATCGT CCAGGCGCAC GGACTTTGTG 
GACCGGAGCC GCTCCTCTCA CCAGCTTCGG GCCCTGGCAG CCGGCCGTGC CTACAACATC 
TCTGTTTTCT CAGTCAAGAG AAACACTAAC AACAAAAATG ACATCAGCAG GCCTGCAGCC 
CTGCTCACCC GCACCCGACC CCGCCCTATT GAAGACTTCG AGGTCACCAA CATTTCAGCC 
AATGCCATCT CAGTGCAGTG GGCTCTTCAT AGGATCCAGC ATGCCACTGT CAGCAGGGTT 
CGAGTGTCTG TCCTCTACCC TGAGGACACT GTGGTCCAGT CCACGGAGGT GGACAGGAGT 
GTGGACCGCC TCACATTTGG GGACCTGCTG CCAGGGAGAA GATACAGTGT GCGGCTAACC 
ACCCTCAGTG GGCCTGGAGG AGCTGAATAT CCTACAGAGA GCCTGGCCTC AGCTCCGCTG 
AACGTGTGGA CCCGGCCTTT GCCTCCAGCA AACCTGACTG CCTCTCGAGT CACAGCGACC 
TCTGCCCATA TGGTCTGGGA CCCGCCCACT CCAGGCATCT CACTGGAGGC TTACGTCATC 
AATGTGACCA CCAGTCAGAA TACCAAGAGC CGCTACATCC CCAATGGGAA GCTGGTGTCC 
TATACGGTGC GTGATCTGAT GCCAGGTCGG CGGTACCAGC TCTCGGTCAC AGCGGTGCAG 
AGCACAGAGC AGGGCCAGCT GCACAGTGAG CCTGCGCACC TCTACATCAT CACCTCCCCC 
AGGGATGGCA CCGACAGGCG CTGGCACCAG GGAGGACACC ACTCACGAAT GCTCAGAAAT 
AGGCCGGCCC CTTTGCGCCT GCCAGAACTG CGCCTCCTCA ATGACCACGG TGCCCCTGAA 
ACACCAACCC AGCCACCCAG GTTCTCAGAG CTTGTAGACG GAAGAGCAAG AGTGAGTGCC 
AGGTTTGGTG GATTGCCCAG CAGAGCAGTA ACTGTGAGAT CACAACCCAC TACTCCGGTG 
CCGCTCAAGA ACACAGAGGC CCCTGAGCAG GCCCGTCTGG CCCTTCAGCT ACCCAAGAAC 
AACAGCAAGG ACACAGAAAG TACCCCTGGC AGCTGTTCAG AAGACACCTG TCAGAATGGA 
GGCACCTGTG TCCCAGGTGC CAATGCCCAC AGCTGTGACT GCAGGCCTGG GTTCAAAGGC 
AGACACTGTG AGCTTGCCTG TGAAAAAGTG CCCCGCCCCT GCACACGGCT GTTCTCTGAG 
ACCAAGTCAT TTCCTGTCTG GGAAGGAGAT GTCTGCCACC ATGTGTATAA GAAAGTCTAC 
AAAGTTCACC AGGACGTGTG TTTTAAGGAG CGCTGCCAGA GCACAAGCCT CAAAAAGCTC 
AAACAGGAAT CAAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Transgenic expression of insulin-response element binding protein-1 in beta-cells reproduces type 2 diabetes.
Endocrinology150(6)2611-7(2009 Jun)
Villafuerte BC,Barati MT,Song Y,Moore JP,Epstein PN,Portillo J

Regulation of insulin-response element binding protein-1 in obesity and diabetes: potential role in impaired insulin-induced gene transcription.
Endocrinology149(10)4829-36(2008 Oct)
Chahal J,Chen CC,Rane MJ,Moore JP,Barati MT,Song Y,Villafuerte BC

An insulin-response element-binding protein that ameliorates hyperglycemia in diabetes.
The Journal of biological chemistry280(20)20010-20(2005 May)
Villafuerte BC,Kaytor EN

Insulin-response element-binding protein 1: a novel Akt substrate involved in transcriptional action of insulin.
The Journal of biological chemistry279(35)36650-9(2004 Aug)
Villafuerte BC,Phillips LS,Rane MJ,Zhao W

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Sned1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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