Agap1 gene cDNA ORF clone, Rattus norvegicus(Norway rat)

mRNA	Protein	Name
XM_017596493.1	XP_017451982.1	arf-GAP with GTPase, ANK repeat and PH domain-containing protein 1 isoform X5
XM_006245400.3	XP_006245462.2	arf-GAP with GTPase, ANK repeat and PH domain-containing protein 1 isoform X7
XM_006245402.3	XP_006245464.2	arf-GAP with GTPase, ANK repeat and PH domain-containing protein 1 isoform X8
NM_001108230.1	NP_001101700.1	arf-GAP with GTPase, ANK repeat and PH domain-containing protein 1
XM_017596491.1	XP_017451980.1	arf-GAP with GTPase, ANK repeat and PH domain-containing protein 1 isoform X2
XM_017596490.1	XP_017451979.1	arf-GAP with GTPase, ANK repeat and PH domain-containing protein 1 isoform X1
XM_017596492.1	XP_017451981.1	arf-GAP with GTPase, ANK repeat and PH domain-containing protein 1 isoform X4
XM_006245403.3	XP_006245465.2	arf-GAP with GTPase, ANK repeat and PH domain-containing protein 1 isoform X9
XM_006245396.3	XP_006245458.2	arf-GAP with GTPase, ANK repeat and PH domain-containing protein 1 isoform X3
XM_017596494.1	XP_017451983.1	arf-GAP with GTPase, ANK repeat and PH domain-containing protein 1 isoform X6

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa11661	NM_001108230.1 Latest version!	Rattus norvegicus ArfGAP with GTPase domain, ankyrin repeat and PH domain 1 (Agap1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
ORa47560	XM_006245396.3 Latest version!	Rattus norvegicus ArfGAP with GTPase domain, ankyrin repeat and PH domain 1 (Agap1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$727.30 $1039.00
ORa47563	XM_006245400.3 Latest version!	Rattus norvegicus ArfGAP with GTPase domain, ankyrin repeat and PH domain 1 (Agap1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$727.30 $1039.00
ORa47565	XM_006245402.3 Latest version!	Rattus norvegicus ArfGAP with GTPase domain, ankyrin repeat and PH domain 1 (Agap1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00
ORa47566	XM_006245403.3 Latest version!	Rattus norvegicus ArfGAP with GTPase domain, ankyrin repeat and PH domain 1 (Agap1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00
ORa62783	XM_017596490.1 Latest version!	Rattus norvegicus ArfGAP with GTPase domain, ankyrin repeat and PH domain 1 (Agap1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$727.30 $1039.00
ORa62784	XM_017596491.1 Latest version!	Rattus norvegicus ArfGAP with GTPase domain, ankyrin repeat and PH domain 1 (Agap1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$727.30 $1039.00
ORa62785	XM_017596492.1 Latest version!	Rattus norvegicus ArfGAP with GTPase domain, ankyrin repeat and PH domain 1 (Agap1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$727.30 $1039.00
ORa62786	XM_017596493.1 Latest version!	Rattus norvegicus ArfGAP with GTPase domain, ankyrin repeat and PH domain 1 (Agap1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$727.30 $1039.00
ORa62787	XM_017596494.1 Latest version!	Rattus norvegicus ArfGAP with GTPase domain, ankyrin repeat and PH domain 1 (Agap1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$727.30 $1039.00

Agap1 ( NM_001108230.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001101700.1 Rattus norvegicus ArfGAP with GTPase domain, ankyrin repeat and PH domain 1 (Agap1), mRNA.

Price & Availability↑

CloneID	ORa11661
Clone ID Related Accession (Same CDS sequence)	NM_001108230.1
Accession Version	NM_001108230.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2010bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-27
Organism	Rattus norvegicus(Norway rat)
Product	arf-GAP with GTPase, ANK repeat and PH domain-containing protein 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CH473997.2. On or before Oct 4, 2007 this sequence version replaced XM_237381.3, XM_001066299.1. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGTGGGTGG ACGCGGTCAT CTTTGTCTTC AGCTTGGAGG ATGAGATCAG TTTCCAGACC 
GTCTACCATT ACTACAGCCG AATGGCCAAC TACAGGAACA CCAGTGAGAT CCCATTAGTG 
CTGGTGGGGA CCCAGGACGC CATAAGTTCC ACCAACCCGA GGGTCATCGA TGACGTCAGG 
GCTCGGAAGC TCTCCAATGA CCTGAAGCGG TGCACGTACT ATGAGACGTG CGCCACCTAT 
GGGCTCAACG TGGAGCGGGT CTTCCAGGAC GTTGCCCAGA AGATTGTTGC CACGAGGAAG 
AAGCAGCAGC TGTCCATAGG CCCCTGCAAG TCCTTACCCA ACTCTCCAAG CCACTCCTCT 
GTGTGTTCTG CCCAAGTGTC CGCAGTACAC ATCAGCCAGA CAAGTAACGG AGGTGGGAGT 
TTAAGCGACT ACTCATCCTC TGTCCCTTCG ACCCCAAGCA CCAGCCAGAA GGAGCTTCGG 
ATCGACGTCC CTCCCACTGC CAACACTCCA ACGCCCGTCC GGAAGCAATC CAAGCGCAGG 
TCCAACCTAT TCACATCTAG GAAAGGGAGC GACCCAGACA AAGAGAAGAA AGGCCTGGAG 
AGCCGCGCGG ACAGCATCGG GAGCGGGCGA GCCATCCCAA TTAAACAGGG CATGCTCCTG 
AAAAGAAGTG GCAAGTCACT AAACAAAGAG TGGAAAAAGA AGTACGTCAC CCTATGCGAC 
AATGGTGTGC TGACCTACCA CCCCAGTTTA CATGACTACA TGCAGAACGT CCATGGCAAA 
GAGATAGACC TCTTGAGAAC CACTGTGAAG GTGCCGGGGA AGAGGCCGCC CCGAGCCACA 
TCAGCCTGTG CGCCGATCTC CAGTCCCAAA ACCAATGGCC TGGCCAAGGA CATGAGCAGT 
TTACACATCT CACCAAATTC AGGTAAGCTC ACAGCTGCGT CTGGGTCTCA GATGGCAAGC 
GGCATCAGCC TGGTCTCCTT CAACAGCCGA CCCGATGGCA TGCACCAGCG CTCCTACTCA 
GTCTCCAGTG CCGACCAGTG GAGTGACGCT ACAGTCATTG CAAACTCAGC CATCAGCAGT 
GAGCAAGAAG AAAACTTGGA GTTTATCATT GTGTCCCTCA CTGGCCAGAC GTGGCACTTT 
GAAGCCACCA CATACGAGGA GCGAGACGCA TGGGTCCAAG CCATCGAGAG CCAGATCCTA 
GCCAGCCTGC AGTCCTGTGA GAGCAGCAAG AATAAGTCCC GACTCACCAG CCAGAGCGAA 
GCCATGGCAC TGCAGTCAAT CCGTAACATG AGAGGGAACT CGCACTGCGT GGACTGTGAC 
ACCCAGAACC CCAACTGGGC CAGTTTGAAC TTGGGAGCCC TCATGTGTAT CGAGTGCTCA 
GGGATCCACC GGAATCTCGG CACCCACCTC TCTCGGGTCC GATCGCTCGA CCTCGACGAC 
TGGCCCATGG AGCTGATCAA GGTGATGTCC TCCATCGGGA ATGAGCTGGC CAACAGCGTG 
TGGGAAGAGG GCAGCCAGGG ACGGACAAAG CCCTCGTTGG ACTCCACCAG GGAAGAAAAG 
GAACGGTGGA TTCGGGCCAA GTATGAGCAG AAACTCTTCC TGGCCCCACT GCCATGCACA 
GAGTTCTCCC TGGGCCAGCA ACTGCTACAT GCCACTGCTG AGGAAGACCT GCGGACTGTC 
ATCCTGCTTC TGGCACACGG GTCACGGGAT GAGGTGAATG AGACCTGTGG GGAAGGAGAT 
GGTCGCACGG CCCTGCACCT GGCCTGCCGC AAGGGCAACG TGGTCCTGGT TCAGCTGCTG 
ATCTGGTACG GGGTGGACGT CATGGCCCGA GACGCCCACG GGAACACAGC ACTGGCCTAT 
GCCCGTCAGG CCTCCAGCCA GGAGTGTATC GACGTGCTTC TACAGTATGG CTGCCCCGAC 
GAGCGCTTTG TGCTCATGGC TACCCCCAAC CTGTCTAGAA AGAACAACAG CAGGAACAAC 
AGTAGCGGCA GGGCGCCCAG TGTCATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001101700.1
CDS	240..2249
Translation	MWVDAVIFVFSLEDEISFQTVYHYYSRMANYRNTSEIPLVLVGTQDAISSTNPRVIDDVRARKLSNDLKRCTYYETCATYGLNVERVFQDVAQKIVATRKKQQLSIGPCKSLPNSPSHSSVCSAQVSAVHISQTSNGGGSLSDYSSSVPSTPSTSQKELRIDVPPTANTPTPVRKQSKRRSNLFTSRKGSDPDKEKKGLESRADSIGSGRAIPIKQGMLLKRSGKSLNKEWKKKYVTLCDNGVLTYHPSLHDYMQNVHGKEIDLLRTTVKVPGKRPPRATSACAPISSPKTNGLAKDMSSLHISPNSGKLTAASGSQMASGISLVSFNSRPDGMHQRSYSVSSADQWSDATVIANSAISSEQEENLEFIIVSLTGQTWHFEATTYEERDAWVQAIESQILASLQSCESSKNKSRLTSQSEAMALQSIRNMRGNSHCVDCDTQNPNWASLNLGALMCIECSGIHRNLGTHLSRVRSLDLDDWPMELIKVMSSIGNELANSVWEEGSQGRTKPSLDSTREEKERWIRAKYEQKLFLAPLPCTEFSLGQQLLHATAEEDLRTVILLLAHGSRDEVNETCGEGDGRTALHLACRKGNVVLVQLLIWYGVDVMARDAHGNTALAYARQASSQECIDVLLQYGCPDERFVLMATPNLSRKNNSRNNSSGRAPSVI

Target ORF information:

RefSeq Version	NM_001108230.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ArfGAP with GTPase domain, ankyrin repeat and PH domain 1 (Agap1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001108230.1

ORF Insert Sequence:

ATGTGGGTGG ACGCGGTCAT CTTTGTCTTC AGCTTGGAGG ATGAGATCAG TTTCCAGACC 
GTCTACCATT ACTACAGCCG AATGGCCAAC TACAGGAACA CCAGTGAGAT CCCATTAGTG 
CTGGTGGGGA CCCAGGACGC CATAAGTTCC ACCAACCCGA GGGTCATCGA TGACGTCAGG 
GCTCGGAAGC TCTCCAATGA CCTGAAGCGG TGCACGTACT ATGAGACGTG CGCCACCTAT 
GGGCTCAACG TGGAGCGGGT CTTCCAGGAC GTTGCCCAGA AGATTGTTGC CACGAGGAAG 
AAGCAGCAGC TGTCCATAGG CCCCTGCAAG TCCTTACCCA ACTCTCCAAG CCACTCCTCT 
GTGTGTTCTG CCCAAGTGTC CGCAGTACAC ATCAGCCAGA CAAGTAACGG AGGTGGGAGT 
TTAAGCGACT ACTCATCCTC TGTCCCTTCG ACCCCAAGCA CCAGCCAGAA GGAGCTTCGG 
ATCGACGTCC CTCCCACTGC CAACACTCCA ACGCCCGTCC GGAAGCAATC CAAGCGCAGG 
TCCAACCTAT TCACATCTAG GAAAGGGAGC GACCCAGACA AAGAGAAGAA AGGCCTGGAG 
AGCCGCGCGG ACAGCATCGG GAGCGGGCGA GCCATCCCAA TTAAACAGGG CATGCTCCTG 
AAAAGAAGTG GCAAGTCACT AAACAAAGAG TGGAAAAAGA AGTACGTCAC CCTATGCGAC 
AATGGTGTGC TGACCTACCA CCCCAGTTTA CATGACTACA TGCAGAACGT CCATGGCAAA 
GAGATAGACC TCTTGAGAAC CACTGTGAAG GTGCCGGGGA AGAGGCCGCC CCGAGCCACA 
TCAGCCTGTG CGCCGATCTC CAGTCCCAAA ACCAATGGCC TGGCCAAGGA CATGAGCAGT 
TTACACATCT CACCAAATTC AGGTAAGCTC ACAGCTGCGT CTGGGTCTCA GATGGCAAGC 
GGCATCAGCC TGGTCTCCTT CAACAGCCGA CCCGATGGCA TGCACCAGCG CTCCTACTCA 
GTCTCCAGTG CCGACCAGTG GAGTGACGCT ACAGTCATTG CAAACTCAGC CATCAGCAGT 
GAGCAAGAAG AAAACTTGGA GTTTATCATT GTGTCCCTCA CTGGCCAGAC GTGGCACTTT 
GAAGCCACCA CATACGAGGA GCGAGACGCA TGGGTCCAAG CCATCGAGAG CCAGATCCTA 
GCCAGCCTGC AGTCCTGTGA GAGCAGCAAG AATAAGTCCC GACTCACCAG CCAGAGCGAA 
GCCATGGCAC TGCAGTCAAT CCGTAACATG AGAGGGAACT CGCACTGCGT GGACTGTGAC 
ACCCAGAACC CCAACTGGGC CAGTTTGAAC TTGGGAGCCC TCATGTGTAT CGAGTGCTCA 
GGGATCCACC GGAATCTCGG CACCCACCTC TCTCGGGTCC GATCGCTCGA CCTCGACGAC 
TGGCCCATGG AGCTGATCAA GGTGATGTCC TCCATCGGGA ATGAGCTGGC CAACAGCGTG 
TGGGAAGAGG GCAGCCAGGG ACGGACAAAG CCCTCGTTGG ACTCCACCAG GGAAGAAAAG 
GAACGGTGGA TTCGGGCCAA GTATGAGCAG AAACTCTTCC TGGCCCCACT GCCATGCACA 
GAGTTCTCCC TGGGCCAGCA ACTGCTACAT GCCACTGCTG AGGAAGACCT GCGGACTGTC 
ATCCTGCTTC TGGCACACGG GTCACGGGAT GAGGTGAATG AGACCTGTGG GGAAGGAGAT 
GGTCGCACGG CCCTGCACCT GGCCTGCCGC AAGGGCAACG TGGTCCTGGT TCAGCTGCTG 
ATCTGGTACG GGGTGGACGT CATGGCCCGA GACGCCCACG GGAACACAGC ACTGGCCTAT 
GCCCGTCAGG CCTCCAGCCA GGAGTGTATC GACGTGCTTC TACAGTATGG CTGCCCCGAC 
GAGCGCTTTG TGCTCATGGC TACCCCCAAC CTGTCTAGAA AGAACAACAG CAGGAACAAC 
AGTAGCGGCA GGGCGCCCAG TGTCATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Agap1 ( XM_006245396.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006245458.2 Rattus norvegicus ArfGAP with GTPase domain, ankyrin repeat and PH domain 1 (Agap1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ORa47560
Clone ID Related Accession (Same CDS sequence)	XM_006245396.3
Accession Version	XM_006245396.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4188bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	arf-GAP with GTPase, ANK repeat and PH domain-containing protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005108.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006245396.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCTTGC AGCAAGCAGG TCCTGGAGAG CGCAGTATAG GATTACTGCG CGAAGCTGAT 
CCGCTCCGCG CGGTGGGGAT CCATCCCAGC GCTTGGGCTT GCCCTCCGCC GCGCTCGCCG 
CCGCCTCCGC GCTCCCGGGA CCCGCAGGCC CCGCTGGGCG CGCGGCCCGC AGTCTCCGCA 
CGTCGGCCTC CGCCCGCCCG CCCCGCCCCG GCGCGCATTT CCATTTTAAA CCGCCGCCCG 
CCCGCCCCTC GACCGACCGC GGCCGGCGCA ACCACCCGCG CGGCACGGAA CCTCGGGAGA 
GGCGGAGGCT CCCGGACTGC CGAGCTGTGC AGGAAAAGGC CCTGGGATGC GCTTCGGCCC 
GGCGCACTAG ACAATGAAGC CGCGGGCGCT GCTGCCCGCC GCGGGCTTTG CGCACCCAGG 
CCGCACCTGC TGGCGAGGCG GGTTCCGTGC CTGAGCGCCC ACCCAGCCCC TCATGGCTCG 
GCTCTCCGGG TGCGGACCCC GGCAGTCGGG CCGCGTGGCA CGCGGACGGG CGGACTCCAG 
GGGCGCTGTT CCCGCAGCCT GAGCGCGGGC CTCGCTGCGC GCGGGGGGCG TGGGCGCCGC 
TCCCCGCCCG CTCGGGCTCT GCACCTGGCC AGCTCTGCGC CTGAGGCGCG CGTCCCGGCA 
GCCGGCGGCG CTGGATGCAG CCGGGCCTCG GAGGGCGGCG AGGCGGCCGC GGCCCCTGGG 
GACATGTACC TGCTGGACGG CAGCGGCGAG CCCGCGTCTT CGCCTGCGGA CACGATGTCG 
CGCGGGATGC CCCCCAGGAA GACGGTCTAC CGCATCTCGG TGACCATGGT CAGGAAGGAG 
CAGCTGGCCG CTCCGGCTCC ACGTCCTACT CGGCGGCCCC GGCCCGCGCG CTCCCCGGAG 
GCGCCCGGGC GGCTGGAGGA GGAGGCCGAG GAGGCGGCGG CTGAGGATCC TGAGATCGGG 
CCGCGCGCCT GGGACTTCCG CGCCTTTCGC ACACGCAGCA CCGGGCAGCT GGAGCTCGGG 
CGGCTGCGGC CGTGCGCACA CGACACAGAA CCCACCGACC TGATGGGTAG CCCCACGACT 
GAGGGGCCCA GCAGTCCTGA TGGGGAGGAG GCACGGGCCG CGCCCCTGCG GCGGAGCCTC 
AGCTTTAGGC ACTGGAGCGA GCCCGAGGTG CCACGGAGCC CTGCGCTGAG CGGCGGGAGG 
CGCCACAGCA GCTCTGGAAG CCTGGCATGG ATGCCCGACG ACGATGTCGA GGCGGACGTC 
GTTCCGGAAC CGGTGGCTGG AGTGCAGCCG GCTGCTGAGA AGCGCAACAC CTTGGACGTG 
GGCGAGGTGC TTAGCAAGAA CGATGCCCTC TCAGACCTGG AGCGCTGGGA GCGCAGCAAG 
AGCAAGAACC GCACGCTGGA CAACAGCGAC CTGCAGCGGC TGGAGCGCGC GCGGGCCGCG 
GCTGCCGGCC CTGGGGCCGC CTCGGAGCAC CGGCTGCTGC GTTTCTTCAG TGGCATCTTC 
GCGCGCAGGG ACGGCGGGCC CTCACCCCGC AGCGGCGCCC TACGCTCGCG CGGTTACTTC 
AGCCTGCGGC GGGCCCCGGC CGACACGCAT TCGTCCAGTG CCGAGAGCAT CGAGGGGTCC 
CCGCGCAGAG ATGCTTTCGT GAACAGCCAG GAATGGACAC TCAGCCGATC AGTACCAGAG 
CTCAAAGTGG GAATTGTGGG TAACCTGGCC AGTGGCAAGT CTGCGCTGGT GCACCGGTAC 
CTGACAGGCA CATACGTGCA GGAGGAGTCT CCGGAAGATA TGGACGCAGG TGGCAGGTTC 
AAGAAGGAGA TTGTTGTTGA TGGACAGAGT TATCTGCTGC TGATTAGGGA TGAAGGGGGT 
CCCCCGGAGG CACAGTTTGC CATGTGGGTG GACGCGGTCA TCTTTGTCTT CAGCTTGGAG 
GATGAGATCA GTTTCCAGAC CGTCTACCAT TACTACAGCC GAATGGCCAA CTACAGGAAC 
ACCAGTGAGA TCCCATTAGT GCTGGTGGGG ACCCAGGACG CCATAAGTTC CACCAACCCG 
AGGGTCATCG ATGACGTCAG GGCTCGGAAG CTCTCCAATG ACCTGAAGCG GTGCACGTAC 
TATGAGACGT GCGCCACCTA TGGGCTCAAC GTGGAGCGGG TCTTCCAGGA CGTTGCCCAG 
AAGATTGTTG CCACGAGGAA GAAGCAGCAG CTGTCCATAG GCCCCTGCAA GTCCTTACCC 
AACTCTCCAA GCCACTCCTC TGTGTGTTCT GCCCAAGTGT CCGCAGTACA CATCAGCCAG 
ACAAGTAACG GAGGTGGGAG TTTAAGCGAC TACTCATCCT CTGTCCCTTC GACCCCAAGC 
ACCAGCCAGA AGGAGCTTCG GATCGACGTC CCTCCCACTG CCAACACTCC AACGCCCGTC 
CGGAAGCAAT CCAAGCGCAG GTCCAACCTA TTCACATCTA GGAAAGGGAG CGACCCAGAC 
AAAGAGAAGA AAGGCCTGGA GAGCCGCGCG GACAGCATCG GGAGCGGGCG AGCCATCCCA 
ATTAAACAGG GCATGCTCCT GAAAAGAAGT GGCAAGTCAC TAAACAAAGA GTGGAAAAAG 
AAGTACGTCA CCCTATGCGA CAATGGTGTG CTGACCTACC ACCCCAGTTT ACATGACTAC 
ATGCAGAACG TCCATGGCAA AGAGATAGAC CTCTTGAGAA CCACTGTGAA GGTGCCGGGG 
AAGAGGCCGC CCCGAGCCAC ATCAGCCTGT GCGCCGATCT CCAGTCCCAA AACCAATGGC 
CTGGCCAAGG ACATGAGCAG TTTACACATC TCACCAAATT CAGGGAATGT CACTAGTGCG 
TCTGGGTCTC AGATGGCAAG CGGCATCAGC CTGGTCTCCT TCAACAGCCG ACCCGATGGC 
ATGCACCAGC GCTCCTACTC AGTCTCCAGT GCCGACCAGT GGAGTGACGC TACAGTCATT 
GCAAACTCAG CCATCAGCAG TGACACGGGG CTGGGTGACT CGGTGTGTTC CAGCCCAAGT 
ATCTCCAGCA GCACCAGCCC CAAGCTCGAC CCACCCCCCT CCCCCCACGC CAACAGAAAG 
AAGCATCGGA GGAAGAAAAG TACCAGCAAC TTCAAGGCCG ACGGGCTCAC GGGCACCGCT 
GAAGGTTCAC ATTCACACTC TGGCCTCAGA CTGAGAGACC CAGTTGCCTG GCGCCCTGGA 
GATCTCCATC CACAGTTCCA TACATCTTTA GGCCAGAAAA GAGCCCCACG GCCCCATCTT 
GGGAGCTTCT GGGTTTCTGA GCAAGAAGAA AACTTGGAGT TTATCATTGT GTCCCTCACT 
GGCCAGACGT GGCACTTTGA AGCCACCACA TACGAGGAGC GAGACGCATG GGTCCAAGCC 
ATCGAGAGCC AGATCCTAGC CAGCCTGCAG TCCTGTGAGA GCAGCAAGAA TAAGTCCCGA 
CTCACCAGCC AGAGCGAAGC CATGGCACTG CAGTCAATCC GTAACATGAG AGGGAACTCG 
CACTGCGTGG ACTGTGACAC CCAGAACCCC AACTGGGCCA GTTTGAACTT GGGAGCCCTC 
ATGTGTATCG AGTGCTCAGG GATCCACCGG AATCTCGGCA CCCACCTCTC TCGGGTCCGA 
TCGCTCGACC TCGACGACTG GCCCATGGAG CTGATCAAGG TGATGTCCTC CATCGGGAAT 
GAGCTGGCCA ACAGCGTGTG GGAAGAGGGC AGCCAGGGAC GGACAAAGCC CTCGTTGGAC 
TCCACCAGGG AAGAAAAGGA ACGGTGGATT CGGGCCAAGT ATGAGCAGAA ACTCTTCCTG 
GCCCCACTGC CATGCACAGA GTTCTCCCTG GGCCAGCAAC TGCTACATGC CACTGCTGAG 
GAAGACCTGC GGACTGTCAT CCTGCTTCTG GCACACGGGT CACGGGATGA GGTGAATGAG 
ACCTGTGGGG AAGGAGATGG TCGCACGGCC CTGCACCTGG CCTGCCGCAA GGGCAACGTG 
GTCCTGGTTC AGCTGCTGAT CTGGTACGGG GTGGACGTCA TGGCCCGAGA CGCCCACGGG 
AACACAGCAC TGGCCTATGC CCGTCAGGCC TCCAGCCAGG AGTGTATCGA CGTGCTTCTA 
CAGTATGGCT GCCCCGACGA GCGCTTTGTG CTCATGGCTA CCCCCAACCT GTCTAGAAAG 
AACAACAGCA GGAACAACAG TAGCGGCAGG GCGCCCAGTG TCATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006245458.2
CDS	2465..6652
Translation	MTLQQAGPGERSIGLLREADPLRAVGIHPSAWACPPPRSPPPPRSRDPQAPLGARPAVSARRPPPARPAPARISILNRRPPAPRPTAAGATTRAARNLGRGGGSRTAELCRKRPWDALRPGALDNEAAGAAARRGLCAPRPHLLARRVPCLSAHPAPHGSALRVRTPAVGPRGTRTGGLQGRCSRSLSAGLAARGGRGRRSPPARALHLASSAPEARVPAAGGAGCSRASEGGEAAAAPGDMYLLDGSGEPASSPADTMSRGMPPRKTVYRISVTMVRKEQLAAPAPRPTRRPRPARSPEAPGRLEEEAEEAAAEDPEIGPRAWDFRAFRTRSTGQLELGRLRPCAHDTEPTDLMGSPTTEGPSSPDGEEARAAPLRRSLSFRHWSEPEVPRSPALSGGRRHSSSGSLAWMPDDDVEADVVPEPVAGVQPAAEKRNTLDVGEVLSKNDALSDLERWERSKSKNRTLDNSDLQRLERARAAAAGPGAASEHRLLRFFSGIFARRDGGPSPRSGALRSRGYFSLRRAPADTHSSSAESIEGSPRRDAFVNSQEWTLSRSVPELKVGIVGNLASGKSALVHRYLTGTYVQEESPEDMDAGGRFKKEIVVDGQSYLLLIRDEGGPPEAQFAMWVDAVIFVFSLEDEISFQTVYHYYSRMANYRNTSEIPLVLVGTQDAISSTNPRVIDDVRARKLSNDLKRCTYYETCATYGLNVERVFQDVAQKIVATRKKQQLSIGPCKSLPNSPSHSSVCSAQVSAVHISQTSNGGGSLSDYSSSVPSTPSTSQKELRIDVPPTANTPTPVRKQSKRRSNLFTSRKGSDPDKEKKGLESRADSIGSGRAIPIKQGMLLKRSGKSLNKEWKKKYVTLCDNGVLTYHPSLHDYMQNVHGKEIDLLRTTVKVPGKRPPRATSACAPISSPKTNGLAKDMSSLHISPNSGNVTSASGSQMASGISLVSFNSRPDGMHQRSYSVSSADQWSDATVIANSAISSDTGLGDSVCSSPSISSSTSPKLDPPPSPHANRKKHRRKKSTSNFKADGLTGTAEGSHSHSGLRLRDPVAWRPGDLHPQFHTSLGQKRAPRPHLGSFWVSEQEENLEFIIVSLTGQTWHFEATTYEERDAWVQAIESQILASLQSCESSKNKSRLTSQSEAMALQSIRNMRGNSHCVDCDTQNPNWASLNLGALMCIECSGIHRNLGTHLSRVRSLDLDDWPMELIKVMSSIGNELANSVWEEGSQGRTKPSLDSTREEKERWIRAKYEQKLFLAPLPCTEFSLGQQLLHATAEEDLRTVILLLAHGSRDEVNETCGEGDGRTALHLACRKGNVVLVQLLIWYGVDVMARDAHGNTALAYARQASSQECIDVLLQYGCPDERFVLMATPNLSRKNNSRNNSSGRAPSVI

Target ORF information:

RefSeq Version	XM_006245396.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ArfGAP with GTPase domain, ankyrin repeat and PH domain 1 (Agap1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006245396.3

ORF Insert Sequence:

ATGACCTTGC AGCAAGCAGG TCCTGGAGAG CGCAGTATAG GATTACTGCG CGAAGCTGAT 
CCGCTCCGCG CGGTGGGGAT CCATCCCAGC GCTTGGGCTT GCCCTCCGCC GCGCTCGCCG 
CCGCCTCCGC GCTCCCGGGA CCCGCAGGCC CCGCTGGGCG CGCGGCCCGC AGTCTCCGCA 
CGTCGGCCTC CGCCCGCCCG CCCCGCCCCG GCGCGCATTT CCATTTTAAA CCGCCGCCCG 
CCCGCCCCTC GACCGACCGC GGCCGGCGCA ACCACCCGCG CGGCACGGAA CCTCGGGAGA 
GGCGGAGGCT CCCGGACTGC CGAGCTGTGC AGGAAAAGGC CCTGGGATGC GCTTCGGCCC 
GGCGCACTAG ACAATGAAGC CGCGGGCGCT GCTGCCCGCC GCGGGCTTTG CGCACCCAGG 
CCGCACCTGC TGGCGAGGCG GGTTCCGTGC CTGAGCGCCC ACCCAGCCCC TCATGGCTCG 
GCTCTCCGGG TGCGGACCCC GGCAGTCGGG CCGCGTGGCA CGCGGACGGG CGGACTCCAG 
GGGCGCTGTT CCCGCAGCCT GAGCGCGGGC CTCGCTGCGC GCGGGGGGCG TGGGCGCCGC 
TCCCCGCCCG CTCGGGCTCT GCACCTGGCC AGCTCTGCGC CTGAGGCGCG CGTCCCGGCA 
GCCGGCGGCG CTGGATGCAG CCGGGCCTCG GAGGGCGGCG AGGCGGCCGC GGCCCCTGGG 
GACATGTACC TGCTGGACGG CAGCGGCGAG CCCGCGTCTT CGCCTGCGGA CACGATGTCG 
CGCGGGATGC CCCCCAGGAA GACGGTCTAC CGCATCTCGG TGACCATGGT CAGGAAGGAG 
CAGCTGGCCG CTCCGGCTCC ACGTCCTACT CGGCGGCCCC GGCCCGCGCG CTCCCCGGAG 
GCGCCCGGGC GGCTGGAGGA GGAGGCCGAG GAGGCGGCGG CTGAGGATCC TGAGATCGGG 
CCGCGCGCCT GGGACTTCCG CGCCTTTCGC ACACGCAGCA CCGGGCAGCT GGAGCTCGGG 
CGGCTGCGGC CGTGCGCACA CGACACAGAA CCCACCGACC TGATGGGTAG CCCCACGACT 
GAGGGGCCCA GCAGTCCTGA TGGGGAGGAG GCACGGGCCG CGCCCCTGCG GCGGAGCCTC 
AGCTTTAGGC ACTGGAGCGA GCCCGAGGTG CCACGGAGCC CTGCGCTGAG CGGCGGGAGG 
CGCCACAGCA GCTCTGGAAG CCTGGCATGG ATGCCCGACG ACGATGTCGA GGCGGACGTC 
GTTCCGGAAC CGGTGGCTGG AGTGCAGCCG GCTGCTGAGA AGCGCAACAC CTTGGACGTG 
GGCGAGGTGC TTAGCAAGAA CGATGCCCTC TCAGACCTGG AGCGCTGGGA GCGCAGCAAG 
AGCAAGAACC GCACGCTGGA CAACAGCGAC CTGCAGCGGC TGGAGCGCGC GCGGGCCGCG 
GCTGCCGGCC CTGGGGCCGC CTCGGAGCAC CGGCTGCTGC GTTTCTTCAG TGGCATCTTC 
GCGCGCAGGG ACGGCGGGCC CTCACCCCGC AGCGGCGCCC TACGCTCGCG CGGTTACTTC 
AGCCTGCGGC GGGCCCCGGC CGACACGCAT TCGTCCAGTG CCGAGAGCAT CGAGGGGTCC 
CCGCGCAGAG ATGCTTTCGT GAACAGCCAG GAATGGACAC TCAGCCGATC AGTACCAGAG 
CTCAAAGTGG GAATTGTGGG TAACCTGGCC AGTGGCAAGT CTGCGCTGGT GCACCGGTAC 
CTGACAGGCA CATACGTGCA GGAGGAGTCT CCGGAAGATA TGGACGCAGG TGGCAGGTTC 
AAGAAGGAGA TTGTTGTTGA TGGACAGAGT TATCTGCTGC TGATTAGGGA TGAAGGGGGT 
CCCCCGGAGG CACAGTTTGC CATGTGGGTG GACGCGGTCA TCTTTGTCTT CAGCTTGGAG 
GATGAGATCA GTTTCCAGAC CGTCTACCAT TACTACAGCC GAATGGCCAA CTACAGGAAC 
ACCAGTGAGA TCCCATTAGT GCTGGTGGGG ACCCAGGACG CCATAAGTTC CACCAACCCG 
AGGGTCATCG ATGACGTCAG GGCTCGGAAG CTCTCCAATG ACCTGAAGCG GTGCACGTAC 
TATGAGACGT GCGCCACCTA TGGGCTCAAC GTGGAGCGGG TCTTCCAGGA CGTTGCCCAG 
AAGATTGTTG CCACGAGGAA GAAGCAGCAG CTGTCCATAG GCCCCTGCAA GTCCTTACCC 
AACTCTCCAA GCCACTCCTC TGTGTGTTCT GCCCAAGTGT CCGCAGTACA CATCAGCCAG 
ACAAGTAACG GAGGTGGGAG TTTAAGCGAC TACTCATCCT CTGTCCCTTC GACCCCAAGC 
ACCAGCCAGA AGGAGCTTCG GATCGACGTC CCTCCCACTG CCAACACTCC AACGCCCGTC 
CGGAAGCAAT CCAAGCGCAG GTCCAACCTA TTCACATCTA GGAAAGGGAG CGACCCAGAC 
AAAGAGAAGA AAGGCCTGGA GAGCCGCGCG GACAGCATCG GGAGCGGGCG AGCCATCCCA 
ATTAAACAGG GCATGCTCCT GAAAAGAAGT GGCAAGTCAC TAAACAAAGA GTGGAAAAAG 
AAGTACGTCA CCCTATGCGA CAATGGTGTG CTGACCTACC ACCCCAGTTT ACATGACTAC 
ATGCAGAACG TCCATGGCAA AGAGATAGAC CTCTTGAGAA CCACTGTGAA GGTGCCGGGG 
AAGAGGCCGC CCCGAGCCAC ATCAGCCTGT GCGCCGATCT CCAGTCCCAA AACCAATGGC 
CTGGCCAAGG ACATGAGCAG TTTACACATC TCACCAAATT CAGGGAATGT CACTAGTGCG 
TCTGGGTCTC AGATGGCAAG CGGCATCAGC CTGGTCTCCT TCAACAGCCG ACCCGATGGC 
ATGCACCAGC GCTCCTACTC AGTCTCCAGT GCCGACCAGT GGAGTGACGC TACAGTCATT 
GCAAACTCAG CCATCAGCAG TGACACGGGG CTGGGTGACT CGGTGTGTTC CAGCCCAAGT 
ATCTCCAGCA GCACCAGCCC CAAGCTCGAC CCACCCCCCT CCCCCCACGC CAACAGAAAG 
AAGCATCGGA GGAAGAAAAG TACCAGCAAC TTCAAGGCCG ACGGGCTCAC GGGCACCGCT 
GAAGGTTCAC ATTCACACTC TGGCCTCAGA CTGAGAGACC CAGTTGCCTG GCGCCCTGGA 
GATCTCCATC CACAGTTCCA TACATCTTTA GGCCAGAAAA GAGCCCCACG GCCCCATCTT 
GGGAGCTTCT GGGTTTCTGA GCAAGAAGAA AACTTGGAGT TTATCATTGT GTCCCTCACT 
GGCCAGACGT GGCACTTTGA AGCCACCACA TACGAGGAGC GAGACGCATG GGTCCAAGCC 
ATCGAGAGCC AGATCCTAGC CAGCCTGCAG TCCTGTGAGA GCAGCAAGAA TAAGTCCCGA 
CTCACCAGCC AGAGCGAAGC CATGGCACTG CAGTCAATCC GTAACATGAG AGGGAACTCG 
CACTGCGTGG ACTGTGACAC CCAGAACCCC AACTGGGCCA GTTTGAACTT GGGAGCCCTC 
ATGTGTATCG AGTGCTCAGG GATCCACCGG AATCTCGGCA CCCACCTCTC TCGGGTCCGA 
TCGCTCGACC TCGACGACTG GCCCATGGAG CTGATCAAGG TGATGTCCTC CATCGGGAAT 
GAGCTGGCCA ACAGCGTGTG GGAAGAGGGC AGCCAGGGAC GGACAAAGCC CTCGTTGGAC 
TCCACCAGGG AAGAAAAGGA ACGGTGGATT CGGGCCAAGT ATGAGCAGAA ACTCTTCCTG 
GCCCCACTGC CATGCACAGA GTTCTCCCTG GGCCAGCAAC TGCTACATGC CACTGCTGAG 
GAAGACCTGC GGACTGTCAT CCTGCTTCTG GCACACGGGT CACGGGATGA GGTGAATGAG 
ACCTGTGGGG AAGGAGATGG TCGCACGGCC CTGCACCTGG CCTGCCGCAA GGGCAACGTG 
GTCCTGGTTC AGCTGCTGAT CTGGTACGGG GTGGACGTCA TGGCCCGAGA CGCCCACGGG 
AACACAGCAC TGGCCTATGC CCGTCAGGCC TCCAGCCAGG AGTGTATCGA CGTGCTTCTA 
CAGTATGGCT GCCCCGACGA GCGCTTTGTG CTCATGGCTA CCCCCAACCT GTCTAGAAAG 
AACAACAGCA GGAACAACAG TAGCGGCAGG GCGCCCAGTG TCATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Agap1 ( XM_006245400.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006245462.2 Rattus norvegicus ArfGAP with GTPase domain, ankyrin repeat and PH domain 1 (Agap1), transcript variant X7, mRNA.

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CloneID	ORa47563
Clone ID Related Accession (Same CDS sequence)	XM_006245400.3
Accession Version	XM_006245400.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4041bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	arf-GAP with GTPase, ANK repeat and PH domain-containing protein 1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005108.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006245400.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCTTGC AGCAAGCAGG TCCTGGAGAG CGCAGTATAG GATTACTGCG CGAAGCTGAT 
CCGCTCCGCG CGGTGGGGAT CCATCCCAGC GCTTGGGCTT GCCCTCCGCC GCGCTCGCCG 
CCGCCTCCGC GCTCCCGGGA CCCGCAGGCC CCGCTGGGCG CGCGGCCCGC AGTCTCCGCA 
CGTCGGCCTC CGCCCGCCCG CCCCGCCCCG GCGCGCATTT CCATTTTAAA CCGCCGCCCG 
CCCGCCCCTC GACCGACCGC GGCCGGCGCA ACCACCCGCG CGGCACGGAA CCTCGGGAGA 
GGCGGAGGCT CCCGGACTGC CGAGCTGTGC AGGAAAAGGC CCTGGGATGC GCTTCGGCCC 
GGCGCACTAG ACAATGAAGC CGCGGGCGCT GCTGCCCGCC GCGGGCTTTG CGCACCCAGG 
CCGCACCTGC TGGCGAGGCG GGTTCCGTGC CTGAGCGCCC ACCCAGCCCC TCATGGCTCG 
GCTCTCCGGG TGCGGACCCC GGCAGTCGGG CCGCGTGGCA CGCGGACGGG CGGACTCCAG 
GGGCGCTGTT CCCGCAGCCT GAGCGCGGGC CTCGCTGCGC GCGGGGGGCG TGGGCGCCGC 
TCCCCGCCCG CTCGGGCTCT GCACCTGGCC AGCTCTGCGC CTGAGGCGCG CGTCCCGGCA 
GCCGGCGGCG CTGGATGCAG CCGGGCCTCG GAGGGCGGCG AGGCGGCCGC GGCCCCTGGG 
GACATGTACC TGCTGGACGG CAGCGGCGAG CCCGCGTCTT CGCCTGCGGA CACGATGTCG 
CGCGGGATGC CCCCCAGGAA GACGGTCTAC CGCATCTCGG TGACCATGGT CAGGAAGGAG 
CAGCTGGCCG CTCCGGCTCC ACGTCCTACT CGGCGGCCCC GGCCCGCGCG CTCCCCGGAG 
GCGCCCGGGC GGCTGGAGGA GGAGGCCGAG GAGGCGGCGG CTGAGGATCC TGAGATCGGG 
CCGCGCGCCT GGGACTTCCG CGCCTTTCGC ACACGCAGCA CCGGGCAGCT GGAGCTCGGG 
CGGCTGCGGC CGTGCGCACA CGACACAGAA CCCACCGACC TGATGGGTAG CCCCACGACT 
GAGGGGCCCA GCAGTCCTGA TGGGGAGGAG GCACGGGCCG CGCCCCTGCG GCGGAGCCTC 
AGCTTTAGGC ACTGGAGCGA GCCCGAGGTG CCACGGAGCC CTGCGCTGAG CGGCGGGAGG 
CGCCACAGCA GCTCTGGAAG CCTGGCATGG ATGCCCGACG ACGATGTCGA GGCGGACGTC 
GTTCCGGAAC CGGTGGCTGG AGTGCAGCCG GCTGCTGAGA AGCGCAACAC CTTGGACGTG 
GGCGAGGTGC TTAGCAAGAA CGATGCCCTC TCAGACCTGG AGCGCTGGGA GCGCAGCAAG 
AGCAAGAACC GCACGCTGGA CAACAGCGAC CTGCAGCGGC TGGAGCGCGC GCGGGCCGCG 
GCTGCCGGCC CTGGGGCCGC CTCGGAGCAC CGGCTGCTGC GTTTCTTCAG TGGCATCTTC 
GCGCGCAGGG ACGGCGGGCC CTCACCCCGC AGCGGCGCCC TACGCTCGCG CGGTTACTTC 
AGCCTGCGGC GGGCCCCGGC CGACACGCAT TCGTCCAGTG CCGAGAGCAT CGAGGGGTCC 
CCGCGCAGAG ATGCTTTCGT GAACAGCCAG GAATGGACAC TCAGCCGATC AGTACCAGAG 
CTCAAAGTGG GAATTGTGGG TAACCTGGCC AGTGGCAAGT CTGCGCTGGT GCACCGGTAC 
CTGACAGGCA CATACGTGCA GGAGGAGTCT CCGGAAGGTG GCAGGTTCAA GAAGGAGATT 
GTTGTTGATG GACAGAGTTA TCTGCTGCTG ATTAGGGATG AAGGGGGTCC CCCGGAGGCA 
CAGTTTGCCA TGTGGGTGGA CGCGGTCATC TTTGTCTTCA GCTTGGAGGA TGAGATCAGT 
TTCCAGACCG TCTACCATTA CTACAGCCGA ATGGCCAACT ACAGGAACAC CAGTGAGATC 
CCATTAGTGC TGGTGGGGAC CCAGGACGCC ATAAGTTCCA CCAACCCGAG GGTCATCGAT 
GACGTCAGGG CTCGGAAGCT CTCCAATGAC CTGAAGCGGT GCACGTACTA TGAGACGTGC 
GCCACCTATG GGCTCAACGT GGAGCGGGTC TTCCAGGACG TTGCCCAGAA GATTGTTGCC 
ACGAGGAAGA AGCAGCAGCT GTCCATAGGC CCCTGCAAGT CCTTACCCAA CTCTCCAAGC 
CACTCCTCTG TGTGTTCTGC CCAAGTGTCC GCAGTACACA TCAGCCAGAC AAGTAACGGA 
GGTGGGAGTT TAAGCGACTA CTCATCCTCT GTCCCTTCGA CCCCAAGCAC CAGCCAGAAG 
GAGCTTCGGA TCGACGTCCC TCCCACTGCC AACACTCCAA CGCCCGTCCG GAAGCAATCC 
AAGCGCAGGT CCAACCTATT CACATCTAGG AAAGGGAGCG ACCCAGACAA AGAGAAGAAA 
GGCCTGGAGA GCCGCGCGGA CAGCATCGGG AGCGGGCGAG CCATCCCAAT TAAACAGGGC 
ATGCTCCTGA AAAGAAGTGG CAAGTCACTA AACAAAGAGT GGAAAAAGAA GTACGTCACC 
CTATGCGACA ATGGTGTGCT GACCTACCAC CCCAGTTTAC ATGACTACAT GCAGAACGTC 
CATGGCAAAG AGATAGACCT CTTGAGAACC ACTGTGAAGG TGCCGGGGAA GAGGCCGCCC 
CGAGCCACAT CAGCCTGTGC GCCGATCTCC AGTCCCAAAA CCAATGGCCT GGCCAAGGAC 
ATGAGCAGTT TACACATCTC ACCAAATTCA GGGAATGTCA CTAGTGCGTC TGGGTCTCAG 
ATGGCAAGCG GCATCAGCCT GGTCTCCTTC AACAGCCGAC CCGATGGCAT GCACCAGCGC 
TCCTACTCAG TCTCCAGTGC CGACCAGTGG AGTGACGCTA CAGTCATTGC AAACTCAGCC 
ATCAGCAGTG ACACGGGGCT GGGTGACTCG GTGTGTTCCA GCCCAAGTAT CTCCAGCAGC 
ACCAGCCCCA AGCTCGACCC ACCCCCCTCC CCCCACGCCA ACAGAAAGAA GCATCGGAGG 
AAGAAAAGTA CCAGCAACTT CAAGGCCGAC GGGCTCACGG GCACCGCTGA AGAGCAAGAA 
GAAAACTTGG AGTTTATCAT TGTGTCCCTC ACTGGCCAGA CGTGGCACTT TGAAGCCACC 
ACATACGAGG AGCGAGACGC ATGGGTCCAA GCCATCGAGA GCCAGATCCT AGCCAGCCTG 
CAGTCCTGTG AGAGCAGCAA GAATAAGTCC CGACTCACCA GCCAGAGCGA AGCCATGGCA 
CTGCAGTCAA TCCGTAACAT GAGAGGGAAC TCGCACTGCG TGGACTGTGA CACCCAGAAC 
CCCAACTGGG CCAGTTTGAA CTTGGGAGCC CTCATGTGTA TCGAGTGCTC AGGGATCCAC 
CGGAATCTCG GCACCCACCT CTCTCGGGTC CGATCGCTCG ACCTCGACGA CTGGCCCATG 
GAGCTGATCA AGGTGATGTC CTCCATCGGG AATGAGCTGG CCAACAGCGT GTGGGAAGAG 
GGCAGCCAGG GACGGACAAA GCCCTCGTTG GACTCCACCA GGGAAGAAAA GGAACGGTGG 
ATTCGGGCCA AGTATGAGCA GAAACTCTTC CTGGCCCCAC TGCCATGCAC AGAGTTCTCC 
CTGGGCCAGC AACTGCTACA TGCCACTGCT GAGGAAGACC TGCGGACTGT CATCCTGCTT 
CTGGCACACG GGTCACGGGA TGAGGTGAAT GAGACCTGTG GGGAAGGAGA TGGTCGCACG 
GCCCTGCACC TGGCCTGCCG CAAGGGCAAC GTGGTCCTGG TTCAGCTGCT GATCTGGTAC 
GGGGTGGACG TCATGGCCCG AGACGCCCAC GGGAACACAG CACTGGCCTA TGCCCGTCAG 
GCCTCCAGCC AGGAGTGTAT CGACGTGCTT CTACAGTATG GCTGCCCCGA CGAGCGCTTT 
GTGCTCATGG CTACCCCCAA CCTGTCTAGA AAGAACAACA GCAGGAACAA CAGTAGCGGC 
AGGGCGCCCA GTGTCATCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006245462.2
CDS	2464..6504
Translation	MTLQQAGPGERSIGLLREADPLRAVGIHPSAWACPPPRSPPPPRSRDPQAPLGARPAVSARRPPPARPAPARISILNRRPPAPRPTAAGATTRAARNLGRGGGSRTAELCRKRPWDALRPGALDNEAAGAAARRGLCAPRPHLLARRVPCLSAHPAPHGSALRVRTPAVGPRGTRTGGLQGRCSRSLSAGLAARGGRGRRSPPARALHLASSAPEARVPAAGGAGCSRASEGGEAAAAPGDMYLLDGSGEPASSPADTMSRGMPPRKTVYRISVTMVRKEQLAAPAPRPTRRPRPARSPEAPGRLEEEAEEAAAEDPEIGPRAWDFRAFRTRSTGQLELGRLRPCAHDTEPTDLMGSPTTEGPSSPDGEEARAAPLRRSLSFRHWSEPEVPRSPALSGGRRHSSSGSLAWMPDDDVEADVVPEPVAGVQPAAEKRNTLDVGEVLSKNDALSDLERWERSKSKNRTLDNSDLQRLERARAAAAGPGAASEHRLLRFFSGIFARRDGGPSPRSGALRSRGYFSLRRAPADTHSSSAESIEGSPRRDAFVNSQEWTLSRSVPELKVGIVGNLASGKSALVHRYLTGTYVQEESPEGGRFKKEIVVDGQSYLLLIRDEGGPPEAQFAMWVDAVIFVFSLEDEISFQTVYHYYSRMANYRNTSEIPLVLVGTQDAISSTNPRVIDDVRARKLSNDLKRCTYYETCATYGLNVERVFQDVAQKIVATRKKQQLSIGPCKSLPNSPSHSSVCSAQVSAVHISQTSNGGGSLSDYSSSVPSTPSTSQKELRIDVPPTANTPTPVRKQSKRRSNLFTSRKGSDPDKEKKGLESRADSIGSGRAIPIKQGMLLKRSGKSLNKEWKKKYVTLCDNGVLTYHPSLHDYMQNVHGKEIDLLRTTVKVPGKRPPRATSACAPISSPKTNGLAKDMSSLHISPNSGNVTSASGSQMASGISLVSFNSRPDGMHQRSYSVSSADQWSDATVIANSAISSDTGLGDSVCSSPSISSSTSPKLDPPPSPHANRKKHRRKKSTSNFKADGLTGTAEEQEENLEFIIVSLTGQTWHFEATTYEERDAWVQAIESQILASLQSCESSKNKSRLTSQSEAMALQSIRNMRGNSHCVDCDTQNPNWASLNLGALMCIECSGIHRNLGTHLSRVRSLDLDDWPMELIKVMSSIGNELANSVWEEGSQGRTKPSLDSTREEKERWIRAKYEQKLFLAPLPCTEFSLGQQLLHATAEEDLRTVILLLAHGSRDEVNETCGEGDGRTALHLACRKGNVVLVQLLIWYGVDVMARDAHGNTALAYARQASSQECIDVLLQYGCPDERFVLMATPNLSRKNNSRNNSSGRAPSVI

Target ORF information:

RefSeq Version	XM_006245400.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ArfGAP with GTPase domain, ankyrin repeat and PH domain 1 (Agap1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006245400.3

ORF Insert Sequence:

ATGACCTTGC AGCAAGCAGG TCCTGGAGAG CGCAGTATAG GATTACTGCG CGAAGCTGAT 
CCGCTCCGCG CGGTGGGGAT CCATCCCAGC GCTTGGGCTT GCCCTCCGCC GCGCTCGCCG 
CCGCCTCCGC GCTCCCGGGA CCCGCAGGCC CCGCTGGGCG CGCGGCCCGC AGTCTCCGCA 
CGTCGGCCTC CGCCCGCCCG CCCCGCCCCG GCGCGCATTT CCATTTTAAA CCGCCGCCCG 
CCCGCCCCTC GACCGACCGC GGCCGGCGCA ACCACCCGCG CGGCACGGAA CCTCGGGAGA 
GGCGGAGGCT CCCGGACTGC CGAGCTGTGC AGGAAAAGGC CCTGGGATGC GCTTCGGCCC 
GGCGCACTAG ACAATGAAGC CGCGGGCGCT GCTGCCCGCC GCGGGCTTTG CGCACCCAGG 
CCGCACCTGC TGGCGAGGCG GGTTCCGTGC CTGAGCGCCC ACCCAGCCCC TCATGGCTCG 
GCTCTCCGGG TGCGGACCCC GGCAGTCGGG CCGCGTGGCA CGCGGACGGG CGGACTCCAG 
GGGCGCTGTT CCCGCAGCCT GAGCGCGGGC CTCGCTGCGC GCGGGGGGCG TGGGCGCCGC 
TCCCCGCCCG CTCGGGCTCT GCACCTGGCC AGCTCTGCGC CTGAGGCGCG CGTCCCGGCA 
GCCGGCGGCG CTGGATGCAG CCGGGCCTCG GAGGGCGGCG AGGCGGCCGC GGCCCCTGGG 
GACATGTACC TGCTGGACGG CAGCGGCGAG CCCGCGTCTT CGCCTGCGGA CACGATGTCG 
CGCGGGATGC CCCCCAGGAA GACGGTCTAC CGCATCTCGG TGACCATGGT CAGGAAGGAG 
CAGCTGGCCG CTCCGGCTCC ACGTCCTACT CGGCGGCCCC GGCCCGCGCG CTCCCCGGAG 
GCGCCCGGGC GGCTGGAGGA GGAGGCCGAG GAGGCGGCGG CTGAGGATCC TGAGATCGGG 
CCGCGCGCCT GGGACTTCCG CGCCTTTCGC ACACGCAGCA CCGGGCAGCT GGAGCTCGGG 
CGGCTGCGGC CGTGCGCACA CGACACAGAA CCCACCGACC TGATGGGTAG CCCCACGACT 
GAGGGGCCCA GCAGTCCTGA TGGGGAGGAG GCACGGGCCG CGCCCCTGCG GCGGAGCCTC 
AGCTTTAGGC ACTGGAGCGA GCCCGAGGTG CCACGGAGCC CTGCGCTGAG CGGCGGGAGG 
CGCCACAGCA GCTCTGGAAG CCTGGCATGG ATGCCCGACG ACGATGTCGA GGCGGACGTC 
GTTCCGGAAC CGGTGGCTGG AGTGCAGCCG GCTGCTGAGA AGCGCAACAC CTTGGACGTG 
GGCGAGGTGC TTAGCAAGAA CGATGCCCTC TCAGACCTGG AGCGCTGGGA GCGCAGCAAG 
AGCAAGAACC GCACGCTGGA CAACAGCGAC CTGCAGCGGC TGGAGCGCGC GCGGGCCGCG 
GCTGCCGGCC CTGGGGCCGC CTCGGAGCAC CGGCTGCTGC GTTTCTTCAG TGGCATCTTC 
GCGCGCAGGG ACGGCGGGCC CTCACCCCGC AGCGGCGCCC TACGCTCGCG CGGTTACTTC 
AGCCTGCGGC GGGCCCCGGC CGACACGCAT TCGTCCAGTG CCGAGAGCAT CGAGGGGTCC 
CCGCGCAGAG ATGCTTTCGT GAACAGCCAG GAATGGACAC TCAGCCGATC AGTACCAGAG 
CTCAAAGTGG GAATTGTGGG TAACCTGGCC AGTGGCAAGT CTGCGCTGGT GCACCGGTAC 
CTGACAGGCA CATACGTGCA GGAGGAGTCT CCGGAAGGTG GCAGGTTCAA GAAGGAGATT 
GTTGTTGATG GACAGAGTTA TCTGCTGCTG ATTAGGGATG AAGGGGGTCC CCCGGAGGCA 
CAGTTTGCCA TGTGGGTGGA CGCGGTCATC TTTGTCTTCA GCTTGGAGGA TGAGATCAGT 
TTCCAGACCG TCTACCATTA CTACAGCCGA ATGGCCAACT ACAGGAACAC CAGTGAGATC 
CCATTAGTGC TGGTGGGGAC CCAGGACGCC ATAAGTTCCA CCAACCCGAG GGTCATCGAT 
GACGTCAGGG CTCGGAAGCT CTCCAATGAC CTGAAGCGGT GCACGTACTA TGAGACGTGC 
GCCACCTATG GGCTCAACGT GGAGCGGGTC TTCCAGGACG TTGCCCAGAA GATTGTTGCC 
ACGAGGAAGA AGCAGCAGCT GTCCATAGGC CCCTGCAAGT CCTTACCCAA CTCTCCAAGC 
CACTCCTCTG TGTGTTCTGC CCAAGTGTCC GCAGTACACA TCAGCCAGAC AAGTAACGGA 
GGTGGGAGTT TAAGCGACTA CTCATCCTCT GTCCCTTCGA CCCCAAGCAC CAGCCAGAAG 
GAGCTTCGGA TCGACGTCCC TCCCACTGCC AACACTCCAA CGCCCGTCCG GAAGCAATCC 
AAGCGCAGGT CCAACCTATT CACATCTAGG AAAGGGAGCG ACCCAGACAA AGAGAAGAAA 
GGCCTGGAGA GCCGCGCGGA CAGCATCGGG AGCGGGCGAG CCATCCCAAT TAAACAGGGC 
ATGCTCCTGA AAAGAAGTGG CAAGTCACTA AACAAAGAGT GGAAAAAGAA GTACGTCACC 
CTATGCGACA ATGGTGTGCT GACCTACCAC CCCAGTTTAC ATGACTACAT GCAGAACGTC 
CATGGCAAAG AGATAGACCT CTTGAGAACC ACTGTGAAGG TGCCGGGGAA GAGGCCGCCC 
CGAGCCACAT CAGCCTGTGC GCCGATCTCC AGTCCCAAAA CCAATGGCCT GGCCAAGGAC 
ATGAGCAGTT TACACATCTC ACCAAATTCA GGGAATGTCA CTAGTGCGTC TGGGTCTCAG 
ATGGCAAGCG GCATCAGCCT GGTCTCCTTC AACAGCCGAC CCGATGGCAT GCACCAGCGC 
TCCTACTCAG TCTCCAGTGC CGACCAGTGG AGTGACGCTA CAGTCATTGC AAACTCAGCC 
ATCAGCAGTG ACACGGGGCT GGGTGACTCG GTGTGTTCCA GCCCAAGTAT CTCCAGCAGC 
ACCAGCCCCA AGCTCGACCC ACCCCCCTCC CCCCACGCCA ACAGAAAGAA GCATCGGAGG 
AAGAAAAGTA CCAGCAACTT CAAGGCCGAC GGGCTCACGG GCACCGCTGA AGAGCAAGAA 
GAAAACTTGG AGTTTATCAT TGTGTCCCTC ACTGGCCAGA CGTGGCACTT TGAAGCCACC 
ACATACGAGG AGCGAGACGC ATGGGTCCAA GCCATCGAGA GCCAGATCCT AGCCAGCCTG 
CAGTCCTGTG AGAGCAGCAA GAATAAGTCC CGACTCACCA GCCAGAGCGA AGCCATGGCA 
CTGCAGTCAA TCCGTAACAT GAGAGGGAAC TCGCACTGCG TGGACTGTGA CACCCAGAAC 
CCCAACTGGG CCAGTTTGAA CTTGGGAGCC CTCATGTGTA TCGAGTGCTC AGGGATCCAC 
CGGAATCTCG GCACCCACCT CTCTCGGGTC CGATCGCTCG ACCTCGACGA CTGGCCCATG 
GAGCTGATCA AGGTGATGTC CTCCATCGGG AATGAGCTGG CCAACAGCGT GTGGGAAGAG 
GGCAGCCAGG GACGGACAAA GCCCTCGTTG GACTCCACCA GGGAAGAAAA GGAACGGTGG 
ATTCGGGCCA AGTATGAGCA GAAACTCTTC CTGGCCCCAC TGCCATGCAC AGAGTTCTCC 
CTGGGCCAGC AACTGCTACA TGCCACTGCT GAGGAAGACC TGCGGACTGT CATCCTGCTT 
CTGGCACACG GGTCACGGGA TGAGGTGAAT GAGACCTGTG GGGAAGGAGA TGGTCGCACG 
GCCCTGCACC TGGCCTGCCG CAAGGGCAAC GTGGTCCTGG TTCAGCTGCT GATCTGGTAC 
GGGGTGGACG TCATGGCCCG AGACGCCCAC GGGAACACAG CACTGGCCTA TGCCCGTCAG 
GCCTCCAGCC AGGAGTGTAT CGACGTGCTT CTACAGTATG GCTGCCCCGA CGAGCGCTTT 
GTGCTCATGG CTACCCCCAA CCTGTCTAGA AAGAACAACA GCAGGAACAA CAGTAGCGGC 
AGGGCGCCCA GTGTCATCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Agap1 ( XM_006245402.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006245464.2 Rattus norvegicus ArfGAP with GTPase domain, ankyrin repeat and PH domain 1 (Agap1), transcript variant X8, mRNA.

Price & Availability↑

CloneID	ORa47565
Clone ID Related Accession (Same CDS sequence)	XM_006245402.3
Accession Version	XM_006245402.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3972bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	arf-GAP with GTPase, ANK repeat and PH domain-containing protein 1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005108.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006245402.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCTTGC AGCAAGCAGG TCCTGGAGAG CGCAGTATAG GATTACTGCG CGAAGCTGAT 
CCGCTCCGCG CGGTGGGGAT CCATCCCAGC GCTTGGGCTT GCCCTCCGCC GCGCTCGCCG 
CCGCCTCCGC GCTCCCGGGA CCCGCAGGCC CCGCTGGGCG CGCGGCCCGC AGTCTCCGCA 
CGTCGGCCTC CGCCCGCCCG CCCCGCCCCG GCGCGCATTT CCATTTTAAA CCGCCGCCCG 
CCCGCCCCTC GACCGACCGC GGCCGGCGCA ACCACCCGCG CGGCACGGAA CCTCGGGAGA 
GGCGGAGGCT CCCGGACTGC CGAGCTGTGC AGGAAAAGGC CCTGGGATGC GCTTCGGCCC 
GGCGCACTAG ACAATGAAGC CGCGGGCGCT GCTGCCCGCC GCGGGCTTTG CGCACCCAGG 
CCGCACCTGC TGGCGAGGCG GGTTCCGTGC CTGAGCGCCC ACCCAGCCCC TCATGGCTCG 
GCTCTCCGGG TGCGGACCCC GGCAGTCGGG CCGCGTGGCA CGCGGACGGG CGGACTCCAG 
GGGCGCTGTT CCCGCAGCCT GAGCGCGGGC CTCGCTGCGC GCGGGGGGCG TGGGCGCCGC 
TCCCCGCCCG CTCGGGCTCT GCACCTGGCC AGCTCTGCGC CTGAGGCGCG CGTCCCGGCA 
GCCGGCGGCG CTGGATGCAG CCGGGCCTCG GAGGGCGGCG AGGCGGCCGC GGCCCCTGGG 
GACATGTACC TGCTGGACGG CAGCGGCGAG CCCGCGTCTT CGCCTGCGGA CACGATGTCG 
CGCGGGATGC CCCCCAGGAA GACGGTCTAC CGCATCTCGG TGACCATGGT CAGGAAGGAG 
CAGCTGGCCG CTCCGGCTCC ACGTCCTACT CGGCGGCCCC GGCCCGCGCG CTCCCCGGAG 
GCGCCCGGGC GGCTGGAGGA GGAGGCCGAG GAGGCGGCGG CTGAGGATCC TGAGATCGGG 
CCGCGCGCCT GGGACTTCCG CGCCTTTCGC ACACGCAGCA CCGGGCAGCT GGAGCTCGGG 
CGGCTGCGGC CGTGCGCACA CGACACAGAA CCCACCGACC TGATGGGTAG CCCCACGACT 
GAGGGGCCCA GCAGTCCTGA TGGGGAGGAG GCACGGGCCG CGCCCCTGCG GCGGAGCCTC 
AGCTTTAGGC ACTGGAGCGA GCCCGAGGTG CCACGGAGCC CTGCGCTGAG CGGCGGGAGG 
CGCCACAGCA GCTCTGGAAG CCTGGCATGG ATGCCCGACG ACGATGTCGA GGCGGACGTC 
GTTCCGGAAC CGGTGGCTGG AGTGCAGCCG GCTGCTGAGA AGCGCAACAC CTTGGACGTG 
GGCGAGGTGC TTAGCAAGAA CGATGCCCTC TCAGACCTGG AGCGCTGGGA GCGCAGCAAG 
AGCAAGAACC GCACGCTGGA CAACAGCGAC CTGCAGCGGC TGGAGCGCGC GCGGGCCGCG 
GCTGCCGGCC CTGGGGCCGC CTCGGAGCAC CGGCTGCTGC GTTTCTTCAG TGGCATCTTC 
GCGCGCAGGG ACGGCGGGCC CTCACCCCGC AGCGGCGCCC TACGCTCGCG CGGTTACTTC 
AGCCTGCGGC GGGCCCCGGC CGACACGCAT TCGTCCAGTG CCGAGAGCAT CGAGGGGTCC 
CCGCGCAGAG ATGCTTTCGT GAACAGCCAG GAATGGACAC TCAGCCGATC AGTACCAGAG 
CTCAAAGTGG GAATTGTGGG TAACCTGGCC AGTGGCAAGT CTGCGCTGGT GCACCGGTAC 
CTGACAGGCA CATACGTGCA GGAGGAGTCT CCGGAAGATA TGGACGCAGG TGGCAGGTTC 
AAGAAGGAGA TTGTTGTTGA TGGACAGAGT TATCTGCTGC TGATTAGGGA TGAAGGGGGT 
CCCCCGGAGG CACAGTTTGC CATGTGGGTG GACGCGGTCA TCTTTGTCTT CAGCTTGGAG 
GATGAGATCA GTTTCCAGAC CGTCTACCAT TACTACAGCC GAATGGCCAA CTACAGGAAC 
ACCAGTGAGA TCCCATTAGT GCTGGTGGGG ACCCAGGACG CCATAAGTTC CACCAACCCG 
AGGGTCATCG ATGACGTCAG GGCTCGGAAG CTCTCCAATG ACCTGAAGCG GTGCACGTAC 
TATGAGACGT GCGCCACCTA TGGGCTCAAC GTGGAGCGGG TCTTCCAGGA CGTTGCCCAG 
AAGATTGTTG CCACGAGGAA GAAGCAGCAG CTGTCCATAG GCCCCTGCAA GTCCTTACCC 
AACTCTCCAA GCCACTCCTC TGTGTGTTCT GCCCAAGTGT CCGCAGTACA CATCAGCCAG 
ACAAGTAACG GAGGTGGGAG TTTAAGCGAC TACTCATCCT CTGTCCCTTC GACCCCAAGC 
ACCAGCCAGA AGGAGCTTCG GATCGACGTC CCTCCCACTG CCAACACTCC AACGCCCGTC 
CGGAAGCAAT CCAAGCGCAG GTCCAACCTA TTCACATCTA GGAAAGGGAG CGACCCAGAC 
AAAGAGAAGA AAGGCCTGGA GAGCCGCGCG GACAGCATCG GGAGCGGGCG AGCCATCCCA 
ATTAAACAGG GCATGCTCCT GAAAAGAAGT GGCAAGTCAC TAAACAAAGA GTGGAAAAAG 
AAGTACGTCA CCCTATGCGA CAATGGTGTG CTGACCTACC ACCCCAGTTT ACATGACTAC 
ATGCAGAACG TCCATGGCAA AGAGATAGAC CTCTTGAGAA CCACTGTGAA GGTGCCGGGG 
AAGAGGCCGC CCCGAGCCAC ATCAGCCTGT GCGCCGATCT CCAGTCCCAA AACCAATGGC 
CTGGCCAAGG ACATGAGCAG TTTACACATC TCACCAAATT CAGACACGGG GCTGGGTGAC 
TCGGTGTGTT CCAGCCCAAG TATCTCCAGC AGCACCAGCC CCAAGCTCGA CCCACCCCCC 
TCCCCCCACG CCAACAGAAA GAAGCATCGG AGGAAGAAAA GTACCAGCAA CTTCAAGGCC 
GACGGGCTCA CGGGCACCGC TGAAGCCAAA CGCAAAGCAT GGAAACTAAA CCGTGTTGGT 
AGCCTGCGAA ACATATACAG CAGCAGCAGC ACCAACACCG AAGAGCAAGA AGAAAACTTG 
GAGTTTATCA TTGTGTCCCT CACTGGCCAG ACGTGGCACT TTGAAGCCAC CACATACGAG 
GAGCGAGACG CATGGGTCCA AGCCATCGAG AGCCAGATCC TAGCCAGCCT GCAGTCCTGT 
GAGAGCAGCA AGAATAAGTC CCGACTCACC AGCCAGAGCG AAGCCATGGC ACTGCAGTCA 
ATCCGTAACA TGAGAGGGAA CTCGCACTGC GTGGACTGTG ACACCCAGAA CCCCAACTGG 
GCCAGTTTGA ACTTGGGAGC CCTCATGTGT ATCGAGTGCT CAGGGATCCA CCGGAATCTC 
GGCACCCACC TCTCTCGGGT CCGATCGCTC GACCTCGACG ACTGGCCCAT GGAGCTGATC 
AAGGTGATGT CCTCCATCGG GAATGAGCTG GCCAACAGCG TGTGGGAAGA GGGCAGCCAG 
GGACGGACAA AGCCCTCGTT GGACTCCACC AGGGAAGAAA AGGAACGGTG GATTCGGGCC 
AAGTATGAGC AGAAACTCTT CCTGGCCCCA CTGCCATGCA CAGAGTTCTC CCTGGGCCAG 
CAACTGCTAC ATGCCACTGC TGAGGAAGAC CTGCGGACTG TCATCCTGCT TCTGGCACAC 
GGGTCACGGG ATGAGGTGAA TGAGACCTGT GGGGAAGGAG ATGGTCGCAC GGCCCTGCAC 
CTGGCCTGCC GCAAGGGCAA CGTGGTCCTG GTTCAGCTGC TGATCTGGTA CGGGGTGGAC 
GTCATGGCCC GAGACGCCCA CGGGAACACA GCACTGGCCT ATGCCCGTCA GGCCTCCAGC 
CAGGAGTGTA TCGACGTGCT TCTACAGTAT GGCTGCCCCG ACGAGCGCTT TGTGCTCATG 
GCTACCCCCA ACCTGTCTAG AAAGAACAAC AGCAGGAACA ACAGTAGCGG CAGGGCGCCC 
AGTGTCATCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006245464.2
CDS	2465..6436
Translation	MTLQQAGPGERSIGLLREADPLRAVGIHPSAWACPPPRSPPPPRSRDPQAPLGARPAVSARRPPPARPAPARISILNRRPPAPRPTAAGATTRAARNLGRGGGSRTAELCRKRPWDALRPGALDNEAAGAAARRGLCAPRPHLLARRVPCLSAHPAPHGSALRVRTPAVGPRGTRTGGLQGRCSRSLSAGLAARGGRGRRSPPARALHLASSAPEARVPAAGGAGCSRASEGGEAAAAPGDMYLLDGSGEPASSPADTMSRGMPPRKTVYRISVTMVRKEQLAAPAPRPTRRPRPARSPEAPGRLEEEAEEAAAEDPEIGPRAWDFRAFRTRSTGQLELGRLRPCAHDTEPTDLMGSPTTEGPSSPDGEEARAAPLRRSLSFRHWSEPEVPRSPALSGGRRHSSSGSLAWMPDDDVEADVVPEPVAGVQPAAEKRNTLDVGEVLSKNDALSDLERWERSKSKNRTLDNSDLQRLERARAAAAGPGAASEHRLLRFFSGIFARRDGGPSPRSGALRSRGYFSLRRAPADTHSSSAESIEGSPRRDAFVNSQEWTLSRSVPELKVGIVGNLASGKSALVHRYLTGTYVQEESPEDMDAGGRFKKEIVVDGQSYLLLIRDEGGPPEAQFAMWVDAVIFVFSLEDEISFQTVYHYYSRMANYRNTSEIPLVLVGTQDAISSTNPRVIDDVRARKLSNDLKRCTYYETCATYGLNVERVFQDVAQKIVATRKKQQLSIGPCKSLPNSPSHSSVCSAQVSAVHISQTSNGGGSLSDYSSSVPSTPSTSQKELRIDVPPTANTPTPVRKQSKRRSNLFTSRKGSDPDKEKKGLESRADSIGSGRAIPIKQGMLLKRSGKSLNKEWKKKYVTLCDNGVLTYHPSLHDYMQNVHGKEIDLLRTTVKVPGKRPPRATSACAPISSPKTNGLAKDMSSLHISPNSDTGLGDSVCSSPSISSSTSPKLDPPPSPHANRKKHRRKKSTSNFKADGLTGTAEAKRKAWKLNRVGSLRNIYSSSSTNTEEQEENLEFIIVSLTGQTWHFEATTYEERDAWVQAIESQILASLQSCESSKNKSRLTSQSEAMALQSIRNMRGNSHCVDCDTQNPNWASLNLGALMCIECSGIHRNLGTHLSRVRSLDLDDWPMELIKVMSSIGNELANSVWEEGSQGRTKPSLDSTREEKERWIRAKYEQKLFLAPLPCTEFSLGQQLLHATAEEDLRTVILLLAHGSRDEVNETCGEGDGRTALHLACRKGNVVLVQLLIWYGVDVMARDAHGNTALAYARQASSQECIDVLLQYGCPDERFVLMATPNLSRKNNSRNNSSGRAPSVI

Target ORF information:

RefSeq Version	XM_006245402.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ArfGAP with GTPase domain, ankyrin repeat and PH domain 1 (Agap1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006245402.3

ORF Insert Sequence:

ATGACCTTGC AGCAAGCAGG TCCTGGAGAG CGCAGTATAG GATTACTGCG CGAAGCTGAT 
CCGCTCCGCG CGGTGGGGAT CCATCCCAGC GCTTGGGCTT GCCCTCCGCC GCGCTCGCCG 
CCGCCTCCGC GCTCCCGGGA CCCGCAGGCC CCGCTGGGCG CGCGGCCCGC AGTCTCCGCA 
CGTCGGCCTC CGCCCGCCCG CCCCGCCCCG GCGCGCATTT CCATTTTAAA CCGCCGCCCG 
CCCGCCCCTC GACCGACCGC GGCCGGCGCA ACCACCCGCG CGGCACGGAA CCTCGGGAGA 
GGCGGAGGCT CCCGGACTGC CGAGCTGTGC AGGAAAAGGC CCTGGGATGC GCTTCGGCCC 
GGCGCACTAG ACAATGAAGC CGCGGGCGCT GCTGCCCGCC GCGGGCTTTG CGCACCCAGG 
CCGCACCTGC TGGCGAGGCG GGTTCCGTGC CTGAGCGCCC ACCCAGCCCC TCATGGCTCG 
GCTCTCCGGG TGCGGACCCC GGCAGTCGGG CCGCGTGGCA CGCGGACGGG CGGACTCCAG 
GGGCGCTGTT CCCGCAGCCT GAGCGCGGGC CTCGCTGCGC GCGGGGGGCG TGGGCGCCGC 
TCCCCGCCCG CTCGGGCTCT GCACCTGGCC AGCTCTGCGC CTGAGGCGCG CGTCCCGGCA 
GCCGGCGGCG CTGGATGCAG CCGGGCCTCG GAGGGCGGCG AGGCGGCCGC GGCCCCTGGG 
GACATGTACC TGCTGGACGG CAGCGGCGAG CCCGCGTCTT CGCCTGCGGA CACGATGTCG 
CGCGGGATGC CCCCCAGGAA GACGGTCTAC CGCATCTCGG TGACCATGGT CAGGAAGGAG 
CAGCTGGCCG CTCCGGCTCC ACGTCCTACT CGGCGGCCCC GGCCCGCGCG CTCCCCGGAG 
GCGCCCGGGC GGCTGGAGGA GGAGGCCGAG GAGGCGGCGG CTGAGGATCC TGAGATCGGG 
CCGCGCGCCT GGGACTTCCG CGCCTTTCGC ACACGCAGCA CCGGGCAGCT GGAGCTCGGG 
CGGCTGCGGC CGTGCGCACA CGACACAGAA CCCACCGACC TGATGGGTAG CCCCACGACT 
GAGGGGCCCA GCAGTCCTGA TGGGGAGGAG GCACGGGCCG CGCCCCTGCG GCGGAGCCTC 
AGCTTTAGGC ACTGGAGCGA GCCCGAGGTG CCACGGAGCC CTGCGCTGAG CGGCGGGAGG 
CGCCACAGCA GCTCTGGAAG CCTGGCATGG ATGCCCGACG ACGATGTCGA GGCGGACGTC 
GTTCCGGAAC CGGTGGCTGG AGTGCAGCCG GCTGCTGAGA AGCGCAACAC CTTGGACGTG 
GGCGAGGTGC TTAGCAAGAA CGATGCCCTC TCAGACCTGG AGCGCTGGGA GCGCAGCAAG 
AGCAAGAACC GCACGCTGGA CAACAGCGAC CTGCAGCGGC TGGAGCGCGC GCGGGCCGCG 
GCTGCCGGCC CTGGGGCCGC CTCGGAGCAC CGGCTGCTGC GTTTCTTCAG TGGCATCTTC 
GCGCGCAGGG ACGGCGGGCC CTCACCCCGC AGCGGCGCCC TACGCTCGCG CGGTTACTTC 
AGCCTGCGGC GGGCCCCGGC CGACACGCAT TCGTCCAGTG CCGAGAGCAT CGAGGGGTCC 
CCGCGCAGAG ATGCTTTCGT GAACAGCCAG GAATGGACAC TCAGCCGATC AGTACCAGAG 
CTCAAAGTGG GAATTGTGGG TAACCTGGCC AGTGGCAAGT CTGCGCTGGT GCACCGGTAC 
CTGACAGGCA CATACGTGCA GGAGGAGTCT CCGGAAGATA TGGACGCAGG TGGCAGGTTC 
AAGAAGGAGA TTGTTGTTGA TGGACAGAGT TATCTGCTGC TGATTAGGGA TGAAGGGGGT 
CCCCCGGAGG CACAGTTTGC CATGTGGGTG GACGCGGTCA TCTTTGTCTT CAGCTTGGAG 
GATGAGATCA GTTTCCAGAC CGTCTACCAT TACTACAGCC GAATGGCCAA CTACAGGAAC 
ACCAGTGAGA TCCCATTAGT GCTGGTGGGG ACCCAGGACG CCATAAGTTC CACCAACCCG 
AGGGTCATCG ATGACGTCAG GGCTCGGAAG CTCTCCAATG ACCTGAAGCG GTGCACGTAC 
TATGAGACGT GCGCCACCTA TGGGCTCAAC GTGGAGCGGG TCTTCCAGGA CGTTGCCCAG 
AAGATTGTTG CCACGAGGAA GAAGCAGCAG CTGTCCATAG GCCCCTGCAA GTCCTTACCC 
AACTCTCCAA GCCACTCCTC TGTGTGTTCT GCCCAAGTGT CCGCAGTACA CATCAGCCAG 
ACAAGTAACG GAGGTGGGAG TTTAAGCGAC TACTCATCCT CTGTCCCTTC GACCCCAAGC 
ACCAGCCAGA AGGAGCTTCG GATCGACGTC CCTCCCACTG CCAACACTCC AACGCCCGTC 
CGGAAGCAAT CCAAGCGCAG GTCCAACCTA TTCACATCTA GGAAAGGGAG CGACCCAGAC 
AAAGAGAAGA AAGGCCTGGA GAGCCGCGCG GACAGCATCG GGAGCGGGCG AGCCATCCCA 
ATTAAACAGG GCATGCTCCT GAAAAGAAGT GGCAAGTCAC TAAACAAAGA GTGGAAAAAG 
AAGTACGTCA CCCTATGCGA CAATGGTGTG CTGACCTACC ACCCCAGTTT ACATGACTAC 
ATGCAGAACG TCCATGGCAA AGAGATAGAC CTCTTGAGAA CCACTGTGAA GGTGCCGGGG 
AAGAGGCCGC CCCGAGCCAC ATCAGCCTGT GCGCCGATCT CCAGTCCCAA AACCAATGGC 
CTGGCCAAGG ACATGAGCAG TTTACACATC TCACCAAATT CAGACACGGG GCTGGGTGAC 
TCGGTGTGTT CCAGCCCAAG TATCTCCAGC AGCACCAGCC CCAAGCTCGA CCCACCCCCC 
TCCCCCCACG CCAACAGAAA GAAGCATCGG AGGAAGAAAA GTACCAGCAA CTTCAAGGCC 
GACGGGCTCA CGGGCACCGC TGAAGCCAAA CGCAAAGCAT GGAAACTAAA CCGTGTTGGT 
AGCCTGCGAA ACATATACAG CAGCAGCAGC ACCAACACCG AAGAGCAAGA AGAAAACTTG 
GAGTTTATCA TTGTGTCCCT CACTGGCCAG ACGTGGCACT TTGAAGCCAC CACATACGAG 
GAGCGAGACG CATGGGTCCA AGCCATCGAG AGCCAGATCC TAGCCAGCCT GCAGTCCTGT 
GAGAGCAGCA AGAATAAGTC CCGACTCACC AGCCAGAGCG AAGCCATGGC ACTGCAGTCA 
ATCCGTAACA TGAGAGGGAA CTCGCACTGC GTGGACTGTG ACACCCAGAA CCCCAACTGG 
GCCAGTTTGA ACTTGGGAGC CCTCATGTGT ATCGAGTGCT CAGGGATCCA CCGGAATCTC 
GGCACCCACC TCTCTCGGGT CCGATCGCTC GACCTCGACG ACTGGCCCAT GGAGCTGATC 
AAGGTGATGT CCTCCATCGG GAATGAGCTG GCCAACAGCG TGTGGGAAGA GGGCAGCCAG 
GGACGGACAA AGCCCTCGTT GGACTCCACC AGGGAAGAAA AGGAACGGTG GATTCGGGCC 
AAGTATGAGC AGAAACTCTT CCTGGCCCCA CTGCCATGCA CAGAGTTCTC CCTGGGCCAG 
CAACTGCTAC ATGCCACTGC TGAGGAAGAC CTGCGGACTG TCATCCTGCT TCTGGCACAC 
GGGTCACGGG ATGAGGTGAA TGAGACCTGT GGGGAAGGAG ATGGTCGCAC GGCCCTGCAC 
CTGGCCTGCC GCAAGGGCAA CGTGGTCCTG GTTCAGCTGC TGATCTGGTA CGGGGTGGAC 
GTCATGGCCC GAGACGCCCA CGGGAACACA GCACTGGCCT ATGCCCGTCA GGCCTCCAGC 
CAGGAGTGTA TCGACGTGCT TCTACAGTAT GGCTGCCCCG ACGAGCGCTT TGTGCTCATG 
GCTACCCCCA ACCTGTCTAG AAAGAACAAC AGCAGGAACA ACAGTAGCGG CAGGGCGCCC 
AGTGTCATCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Agap1 ( XM_006245403.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006245465.2 Rattus norvegicus ArfGAP with GTPase domain, ankyrin repeat and PH domain 1 (Agap1), transcript variant X9, mRNA.

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CloneID	ORa47566
Clone ID Related Accession (Same CDS sequence)	XM_006245403.3
Accession Version	XM_006245403.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3882bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	arf-GAP with GTPase, ANK repeat and PH domain-containing protein 1 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005108.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006245403.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCTTGC AGCAAGCAGG TCCTGGAGAG CGCAGTATAG GATTACTGCG CGAAGCTGAT 
CCGCTCCGCG CGGTGGGGAT CCATCCCAGC GCTTGGGCTT GCCCTCCGCC GCGCTCGCCG 
CCGCCTCCGC GCTCCCGGGA CCCGCAGGCC CCGCTGGGCG CGCGGCCCGC AGTCTCCGCA 
CGTCGGCCTC CGCCCGCCCG CCCCGCCCCG GCGCGCATTT CCATTTTAAA CCGCCGCCCG 
CCCGCCCCTC GACCGACCGC GGCCGGCGCA ACCACCCGCG CGGCACGGAA CCTCGGGAGA 
GGCGGAGGCT CCCGGACTGC CGAGCTGTGC AGGAAAAGGC CCTGGGATGC GCTTCGGCCC 
GGCGCACTAG ACAATGAAGC CGCGGGCGCT GCTGCCCGCC GCGGGCTTTG CGCACCCAGG 
CCGCACCTGC TGGCGAGGCG GGTTCCGTGC CTGAGCGCCC ACCCAGCCCC TCATGGCTCG 
GCTCTCCGGG TGCGGACCCC GGCAGTCGGG CCGCGTGGCA CGCGGACGGG CGGACTCCAG 
GGGCGCTGTT CCCGCAGCCT GAGCGCGGGC CTCGCTGCGC GCGGGGGGCG TGGGCGCCGC 
TCCCCGCCCG CTCGGGCTCT GCACCTGGCC AGCTCTGCGC CTGAGGCGCG CGTCCCGGCA 
GCCGGCGGCG CTGGATGCAG CCGGGCCTCG GAGGGCGGCG AGGCGGCCGC GGCCCCTGGG 
GACATGTACC TGCTGGACGG CAGCGGCGAG CCCGCGTCTT CGCCTGCGGA CACGATGTCG 
CGCGGGATGC CCCCCAGGAA GACGGTCTAC CGCATCTCGG TGACCATGGT CAGGAAGGAG 
CAGCTGGCCG CTCCGGCTCC ACGTCCTACT CGGCGGCCCC GGCCCGCGCG CTCCCCGGAG 
GCGCCCGGGC GGCTGGAGGA GGAGGCCGAG GAGGCGGCGG CTGAGGATCC TGAGATCGGG 
CCGCGCGCCT GGGACTTCCG CGCCTTTCGC ACACGCAGCA CCGGGCAGCT GGAGCTCGGG 
CGGCTGCGGC CGTGCGCACA CGACACAGAA CCCACCGACC TGATGGGTAG CCCCACGACT 
GAGGGGCCCA GCAGTCCTGA TGGGGAGGAG GCACGGGCCG CGCCCCTGCG GCGGAGCCTC 
AGCTTTAGGC ACTGGAGCGA GCCCGAGGTG CCACGGAGCC CTGCGCTGAG CGGCGGGAGG 
CGCCACAGCA GCTCTGGAAG CCTGGCATGG ATGCCCGACG ACGATGTCGA GGCGGACGTC 
GTTCCGGAAC CGGTGGCTGG AGTGCAGCCG GCTGCTGAGA AGCGCAACAC CTTGGACGTG 
GGCGAGGTGC TTAGCAAGAA CGATGCCCTC TCAGACCTGG AGCGCTGGGA GCGCAGCAAG 
AGCAAGAACC GCACGCTGGA CAACAGCGAC CTGCAGCGGC TGGAGCGCGC GCGGGCCGCG 
GCTGCCGGCC CTGGGGCCGC CTCGGAGCAC CGGCTGCTGC GTTTCTTCAG TGGCATCTTC 
GCGCGCAGGG ACGGCGGGCC CTCACCCCGC AGCGGCGCCC TACGCTCGCG CGGTTACTTC 
AGCCTGCGGC GGGCCCCGGC CGACACGCAT TCGTCCAGTG CCGAGAGCAT CGAGGGGTCC 
CCGCGCAGAG ATGCTTTCGT GAACAGCCAG GAATGGACAC TCAGCCGATC AGTACCAGAG 
CTCAAAGTGG GAATTGTGGG TAACCTGGCC AGTGGCAAGT CTGCGCTGGT GCACCGGTAC 
CTGACAGGCA CATACGTGCA GGAGGAGTCT CCGGAAGGTG GCAGGTTCAA GAAGGAGATT 
GTTGTTGATG GACAGAGTTA TCTGCTGCTG ATTAGGGATG AAGGGGGTCC CCCGGAGGCA 
CAGTTTGCCA TGTGGGTGGA CGCGGTCATC TTTGTCTTCA GCTTGGAGGA TGAGATCAGT 
TTCCAGACCG TCTACCATTA CTACAGCCGA ATGGCCAACT ACAGGAACAC CAGTGAGATC 
CCATTAGTGC TGGTGGGGAC CCAGGACGCC ATAAGTTCCA CCAACCCGAG GGTCATCGAT 
GACGTCAGGG CTCGGAAGCT CTCCAATGAC CTGAAGCGGT GCACGTACTA TGAGACGTGC 
GCCACCTATG GGCTCAACGT GGAGCGGGTC TTCCAGGACG TTGCCCAGAA GATTGTTGCC 
ACGAGGAAGA AGCAGCAGCT GTCCATAGGC CCCTGCAAGT CCTTACCCAA CTCTCCAAGC 
CACTCCTCTG TGTGTTCTGC CCAAGTGTCC GCAGTACACA TCAGCCAGAC AAGTAACGGA 
GGTGGGAGTT TAAGCGACTA CTCATCCTCT GTCCCTTCGA CCCCAAGCAC CAGCCAGAAG 
GAGCTTCGGA TCGACGTCCC TCCCACTGCC AACACTCCAA CGCCCGTCCG GAAGCAATCC 
AAGCGCAGGT CCAACCTATT CACATCTAGG AAAGGGAGCG ACCCAGACAA AGAGAAGAAA 
GGCCTGGAGA GCCGCGCGGA CAGCATCGGG AGCGGGCGAG CCATCCCAAT TAAACAGGGC 
ATGCTCCTGA AAAGAAGTGG CAAGTCACTA AACAAAGAGT GGAAAAAGAA GTACGTCACC 
CTATGCGACA ATGGTGTGCT GACCTACCAC CCCAGTTTAC ATGACTACAT GCAGAACGTC 
CATGGCAAAG AGATAGACCT CTTGAGAACC ACTGTGAAGG TGCCGGGGAA GAGGCCGCCC 
CGAGCCACAT CAGCCTGTGC GCCGATCTCC AGTCCCAAAA CCAATGGCCT GGCCAAGGAC 
ATGAGCAGTT TACACATCTC ACCAAATTCA GACACGGGGC TGGGTGACTC GGTGTGTTCC 
AGCCCAAGTA TCTCCAGCAG CACCAGCCCC AAGCTCGACC CACCCCCCTC CCCCCACGCC 
AACAGAAAGA AGCATCGGAG GAAGAAAAGT ACCAGCAACT TCAAGGCCGA CGGGCTCACG 
GGCACCGCTG AAGAGCAAGA AGAAAACTTG GAGTTTATCA TTGTGTCCCT CACTGGCCAG 
ACGTGGCACT TTGAAGCCAC CACATACGAG GAGCGAGACG CATGGGTCCA AGCCATCGAG 
AGCCAGATCC TAGCCAGCCT GCAGTCCTGT GAGAGCAGCA AGAATAAGTC CCGACTCACC 
AGCCAGAGCG AAGCCATGGC ACTGCAGTCA ATCCGTAACA TGAGAGGGAA CTCGCACTGC 
GTGGACTGTG ACACCCAGAA CCCCAACTGG GCCAGTTTGA ACTTGGGAGC CCTCATGTGT 
ATCGAGTGCT CAGGGATCCA CCGGAATCTC GGCACCCACC TCTCTCGGGT CCGATCGCTC 
GACCTCGACG ACTGGCCCAT GGAGCTGATC AAGGTGATGT CCTCCATCGG GAATGAGCTG 
GCCAACAGCG TGTGGGAAGA GGGCAGCCAG GGACGGACAA AGCCCTCGTT GGACTCCACC 
AGGGAAGAAA AGGAACGGTG GATTCGGGCC AAGTATGAGC AGAAACTCTT CCTGGCCCCA 
CTGCCATGCA CAGAGTTCTC CCTGGGCCAG CAACTGCTAC ATGCCACTGC TGAGGAAGAC 
CTGCGGACTG TCATCCTGCT TCTGGCACAC GGGTCACGGG ATGAGGTGAA TGAGACCTGT 
GGGGAAGGAG ATGGTCGCAC GGCCCTGCAC CTGGCCTGCC GCAAGGGCAA CGTGGTCCTG 
GTTCAGCTGC TGATCTGGTA CGGGGTGGAC GTCATGGCCC GAGACGCCCA CGGGAACACA 
GCACTGGCCT ATGCCCGTCA GGCCTCCAGC CAGGAGTGTA TCGACGTGCT TCTACAGTAT 
GGCTGCCCCG ACGAGCGCTT TGTGCTCATG GCTACCCCCA ACCTGTCTAG AAAGAACAAC 
AGCAGGAACA ACAGTAGCGG CAGGGCGCCC AGTGTCATCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006245465.2
CDS	2464..6345
Translation	MTLQQAGPGERSIGLLREADPLRAVGIHPSAWACPPPRSPPPPRSRDPQAPLGARPAVSARRPPPARPAPARISILNRRPPAPRPTAAGATTRAARNLGRGGGSRTAELCRKRPWDALRPGALDNEAAGAAARRGLCAPRPHLLARRVPCLSAHPAPHGSALRVRTPAVGPRGTRTGGLQGRCSRSLSAGLAARGGRGRRSPPARALHLASSAPEARVPAAGGAGCSRASEGGEAAAAPGDMYLLDGSGEPASSPADTMSRGMPPRKTVYRISVTMVRKEQLAAPAPRPTRRPRPARSPEAPGRLEEEAEEAAAEDPEIGPRAWDFRAFRTRSTGQLELGRLRPCAHDTEPTDLMGSPTTEGPSSPDGEEARAAPLRRSLSFRHWSEPEVPRSPALSGGRRHSSSGSLAWMPDDDVEADVVPEPVAGVQPAAEKRNTLDVGEVLSKNDALSDLERWERSKSKNRTLDNSDLQRLERARAAAAGPGAASEHRLLRFFSGIFARRDGGPSPRSGALRSRGYFSLRRAPADTHSSSAESIEGSPRRDAFVNSQEWTLSRSVPELKVGIVGNLASGKSALVHRYLTGTYVQEESPEGGRFKKEIVVDGQSYLLLIRDEGGPPEAQFAMWVDAVIFVFSLEDEISFQTVYHYYSRMANYRNTSEIPLVLVGTQDAISSTNPRVIDDVRARKLSNDLKRCTYYETCATYGLNVERVFQDVAQKIVATRKKQQLSIGPCKSLPNSPSHSSVCSAQVSAVHISQTSNGGGSLSDYSSSVPSTPSTSQKELRIDVPPTANTPTPVRKQSKRRSNLFTSRKGSDPDKEKKGLESRADSIGSGRAIPIKQGMLLKRSGKSLNKEWKKKYVTLCDNGVLTYHPSLHDYMQNVHGKEIDLLRTTVKVPGKRPPRATSACAPISSPKTNGLAKDMSSLHISPNSDTGLGDSVCSSPSISSSTSPKLDPPPSPHANRKKHRRKKSTSNFKADGLTGTAEEQEENLEFIIVSLTGQTWHFEATTYEERDAWVQAIESQILASLQSCESSKNKSRLTSQSEAMALQSIRNMRGNSHCVDCDTQNPNWASLNLGALMCIECSGIHRNLGTHLSRVRSLDLDDWPMELIKVMSSIGNELANSVWEEGSQGRTKPSLDSTREEKERWIRAKYEQKLFLAPLPCTEFSLGQQLLHATAEEDLRTVILLLAHGSRDEVNETCGEGDGRTALHLACRKGNVVLVQLLIWYGVDVMARDAHGNTALAYARQASSQECIDVLLQYGCPDERFVLMATPNLSRKNNSRNNSSGRAPSVI

Target ORF information:

RefSeq Version	XM_006245403.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ArfGAP with GTPase domain, ankyrin repeat and PH domain 1 (Agap1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006245403.3

ORF Insert Sequence:

ATGACCTTGC AGCAAGCAGG TCCTGGAGAG CGCAGTATAG GATTACTGCG CGAAGCTGAT 
CCGCTCCGCG CGGTGGGGAT CCATCCCAGC GCTTGGGCTT GCCCTCCGCC GCGCTCGCCG 
CCGCCTCCGC GCTCCCGGGA CCCGCAGGCC CCGCTGGGCG CGCGGCCCGC AGTCTCCGCA 
CGTCGGCCTC CGCCCGCCCG CCCCGCCCCG GCGCGCATTT CCATTTTAAA CCGCCGCCCG 
CCCGCCCCTC GACCGACCGC GGCCGGCGCA ACCACCCGCG CGGCACGGAA CCTCGGGAGA 
GGCGGAGGCT CCCGGACTGC CGAGCTGTGC AGGAAAAGGC CCTGGGATGC GCTTCGGCCC 
GGCGCACTAG ACAATGAAGC CGCGGGCGCT GCTGCCCGCC GCGGGCTTTG CGCACCCAGG 
CCGCACCTGC TGGCGAGGCG GGTTCCGTGC CTGAGCGCCC ACCCAGCCCC TCATGGCTCG 
GCTCTCCGGG TGCGGACCCC GGCAGTCGGG CCGCGTGGCA CGCGGACGGG CGGACTCCAG 
GGGCGCTGTT CCCGCAGCCT GAGCGCGGGC CTCGCTGCGC GCGGGGGGCG TGGGCGCCGC 
TCCCCGCCCG CTCGGGCTCT GCACCTGGCC AGCTCTGCGC CTGAGGCGCG CGTCCCGGCA 
GCCGGCGGCG CTGGATGCAG CCGGGCCTCG GAGGGCGGCG AGGCGGCCGC GGCCCCTGGG 
GACATGTACC TGCTGGACGG CAGCGGCGAG CCCGCGTCTT CGCCTGCGGA CACGATGTCG 
CGCGGGATGC CCCCCAGGAA GACGGTCTAC CGCATCTCGG TGACCATGGT CAGGAAGGAG 
CAGCTGGCCG CTCCGGCTCC ACGTCCTACT CGGCGGCCCC GGCCCGCGCG CTCCCCGGAG 
GCGCCCGGGC GGCTGGAGGA GGAGGCCGAG GAGGCGGCGG CTGAGGATCC TGAGATCGGG 
CCGCGCGCCT GGGACTTCCG CGCCTTTCGC ACACGCAGCA CCGGGCAGCT GGAGCTCGGG 
CGGCTGCGGC CGTGCGCACA CGACACAGAA CCCACCGACC TGATGGGTAG CCCCACGACT 
GAGGGGCCCA GCAGTCCTGA TGGGGAGGAG GCACGGGCCG CGCCCCTGCG GCGGAGCCTC 
AGCTTTAGGC ACTGGAGCGA GCCCGAGGTG CCACGGAGCC CTGCGCTGAG CGGCGGGAGG 
CGCCACAGCA GCTCTGGAAG CCTGGCATGG ATGCCCGACG ACGATGTCGA GGCGGACGTC 
GTTCCGGAAC CGGTGGCTGG AGTGCAGCCG GCTGCTGAGA AGCGCAACAC CTTGGACGTG 
GGCGAGGTGC TTAGCAAGAA CGATGCCCTC TCAGACCTGG AGCGCTGGGA GCGCAGCAAG 
AGCAAGAACC GCACGCTGGA CAACAGCGAC CTGCAGCGGC TGGAGCGCGC GCGGGCCGCG 
GCTGCCGGCC CTGGGGCCGC CTCGGAGCAC CGGCTGCTGC GTTTCTTCAG TGGCATCTTC 
GCGCGCAGGG ACGGCGGGCC CTCACCCCGC AGCGGCGCCC TACGCTCGCG CGGTTACTTC 
AGCCTGCGGC GGGCCCCGGC CGACACGCAT TCGTCCAGTG CCGAGAGCAT CGAGGGGTCC 
CCGCGCAGAG ATGCTTTCGT GAACAGCCAG GAATGGACAC TCAGCCGATC AGTACCAGAG 
CTCAAAGTGG GAATTGTGGG TAACCTGGCC AGTGGCAAGT CTGCGCTGGT GCACCGGTAC 
CTGACAGGCA CATACGTGCA GGAGGAGTCT CCGGAAGGTG GCAGGTTCAA GAAGGAGATT 
GTTGTTGATG GACAGAGTTA TCTGCTGCTG ATTAGGGATG AAGGGGGTCC CCCGGAGGCA 
CAGTTTGCCA TGTGGGTGGA CGCGGTCATC TTTGTCTTCA GCTTGGAGGA TGAGATCAGT 
TTCCAGACCG TCTACCATTA CTACAGCCGA ATGGCCAACT ACAGGAACAC CAGTGAGATC 
CCATTAGTGC TGGTGGGGAC CCAGGACGCC ATAAGTTCCA CCAACCCGAG GGTCATCGAT 
GACGTCAGGG CTCGGAAGCT CTCCAATGAC CTGAAGCGGT GCACGTACTA TGAGACGTGC 
GCCACCTATG GGCTCAACGT GGAGCGGGTC TTCCAGGACG TTGCCCAGAA GATTGTTGCC 
ACGAGGAAGA AGCAGCAGCT GTCCATAGGC CCCTGCAAGT CCTTACCCAA CTCTCCAAGC 
CACTCCTCTG TGTGTTCTGC CCAAGTGTCC GCAGTACACA TCAGCCAGAC AAGTAACGGA 
GGTGGGAGTT TAAGCGACTA CTCATCCTCT GTCCCTTCGA CCCCAAGCAC CAGCCAGAAG 
GAGCTTCGGA TCGACGTCCC TCCCACTGCC AACACTCCAA CGCCCGTCCG GAAGCAATCC 
AAGCGCAGGT CCAACCTATT CACATCTAGG AAAGGGAGCG ACCCAGACAA AGAGAAGAAA 
GGCCTGGAGA GCCGCGCGGA CAGCATCGGG AGCGGGCGAG CCATCCCAAT TAAACAGGGC 
ATGCTCCTGA AAAGAAGTGG CAAGTCACTA AACAAAGAGT GGAAAAAGAA GTACGTCACC 
CTATGCGACA ATGGTGTGCT GACCTACCAC CCCAGTTTAC ATGACTACAT GCAGAACGTC 
CATGGCAAAG AGATAGACCT CTTGAGAACC ACTGTGAAGG TGCCGGGGAA GAGGCCGCCC 
CGAGCCACAT CAGCCTGTGC GCCGATCTCC AGTCCCAAAA CCAATGGCCT GGCCAAGGAC 
ATGAGCAGTT TACACATCTC ACCAAATTCA GACACGGGGC TGGGTGACTC GGTGTGTTCC 
AGCCCAAGTA TCTCCAGCAG CACCAGCCCC AAGCTCGACC CACCCCCCTC CCCCCACGCC 
AACAGAAAGA AGCATCGGAG GAAGAAAAGT ACCAGCAACT TCAAGGCCGA CGGGCTCACG 
GGCACCGCTG AAGAGCAAGA AGAAAACTTG GAGTTTATCA TTGTGTCCCT CACTGGCCAG 
ACGTGGCACT TTGAAGCCAC CACATACGAG GAGCGAGACG CATGGGTCCA AGCCATCGAG 
AGCCAGATCC TAGCCAGCCT GCAGTCCTGT GAGAGCAGCA AGAATAAGTC CCGACTCACC 
AGCCAGAGCG AAGCCATGGC ACTGCAGTCA ATCCGTAACA TGAGAGGGAA CTCGCACTGC 
GTGGACTGTG ACACCCAGAA CCCCAACTGG GCCAGTTTGA ACTTGGGAGC CCTCATGTGT 
ATCGAGTGCT CAGGGATCCA CCGGAATCTC GGCACCCACC TCTCTCGGGT CCGATCGCTC 
GACCTCGACG ACTGGCCCAT GGAGCTGATC AAGGTGATGT CCTCCATCGG GAATGAGCTG 
GCCAACAGCG TGTGGGAAGA GGGCAGCCAG GGACGGACAA AGCCCTCGTT GGACTCCACC 
AGGGAAGAAA AGGAACGGTG GATTCGGGCC AAGTATGAGC AGAAACTCTT CCTGGCCCCA 
CTGCCATGCA CAGAGTTCTC CCTGGGCCAG CAACTGCTAC ATGCCACTGC TGAGGAAGAC 
CTGCGGACTG TCATCCTGCT TCTGGCACAC GGGTCACGGG ATGAGGTGAA TGAGACCTGT 
GGGGAAGGAG ATGGTCGCAC GGCCCTGCAC CTGGCCTGCC GCAAGGGCAA CGTGGTCCTG 
GTTCAGCTGC TGATCTGGTA CGGGGTGGAC GTCATGGCCC GAGACGCCCA CGGGAACACA 
GCACTGGCCT ATGCCCGTCA GGCCTCCAGC CAGGAGTGTA TCGACGTGCT TCTACAGTAT 
GGCTGCCCCG ACGAGCGCTT TGTGCTCATG GCTACCCCCA ACCTGTCTAG AAAGAACAAC 
AGCAGGAACA ACAGTAGCGG CAGGGCGCCC AGTGTCATCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Agap1 ( XM_017596490.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017451979.1 Rattus norvegicus ArfGAP with GTPase domain, ankyrin repeat and PH domain 1 (Agap1), transcript variant X1, mRNA.

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CloneID	ORa62783
Clone ID Related Accession (Same CDS sequence)	XM_017596490.1
Accession Version	XM_017596490.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4266bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	arf-GAP with GTPase, ANK repeat and PH domain-containing protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005108.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCTTGC AGCAAGCAGG TCCTGGAGAG CGCAGTATAG GATTACTGCG CGAAGCTGAT 
CCGCTCCGCG CGGTGGGGAT CCATCCCAGC GCTTGGGCTT GCCCTCCGCC GCGCTCGCCG 
CCGCCTCCGC GCTCCCGGGA CCCGCAGGCC CCGCTGGGCG CGCGGCCCGC AGTCTCCGCA 
CGTCGGCCTC CGCCCGCCCG CCCCGCCCCG GCGCGCATTT CCATTTTAAA CCGCCGCCCG 
CCCGCCCCTC GACCGACCGC GGCCGGCGCA ACCACCCGCG CGGCACGGAA CCTCGGGAGA 
GGCGGAGGCT CCCGGACTGC CGAGCTGTGC AGGAAAAGGC CCTGGGATGC GCTTCGGCCC 
GGCGCACTAG ACAATGAAGC CGCGGGCGCT GCTGCCCGCC GCGGGCTTTG CGCACCCAGG 
CCGCACCTGC TGGCGAGGCG GGTTCCGTGC CTGAGCGCCC ACCCAGCCCC TCATGGCTCG 
GCTCTCCGGG TGCGGACCCC GGCAGTCGGG CCGCGTGGCA CGCGGACGGG CGGACTCCAG 
GGGCGCTGTT CCCGCAGCCT GAGCGCGGGC CTCGCTGCGC GCGGGGGGCG TGGGCGCCGC 
TCCCCGCCCG CTCGGGCTCT GCACCTGGCC AGCTCTGCGC CTGAGGCGCG CGTCCCGGCA 
GCCGGCGGCG CTGGATGCAG CCGGGCCTCG GAGGGCGGCG AGGCGGCCGC GGCCCCTGGG 
GACATGTACC TGCTGGACGG CAGCGGCGAG CCCGCGTCTT CGCCTGCGGA CACGATGTCG 
CGCGGGATGC CCCCCAGGAA GACGGTCTAC CGCATCTCGG TGACCATGGT CAGGAAGGAG 
CAGCTGGCCG CTCCGGCTCC ACGTCCTACT CGGCGGCCCC GGCCCGCGCG CTCCCCGGAG 
GCGCCCGGGC GGCTGGAGGA GGAGGCCGAG GAGGCGGCGG CTGAGGATCC TGAGATCGGG 
CCGCGCGCCT GGGACTTCCG CGCCTTTCGC ACACGCAGCA CCGGGCAGCT GGAGCTCGGG 
CGGCTGCGGC CGTGCGCACA CGACACAGAA CCCACCGACC TGATGGGTAG CCCCACGACT 
GAGGGGCCCA GCAGTCCTGA TGGGGAGGAG GCACGGGCCG CGCCCCTGCG GCGGAGCCTC 
AGCTTTAGGC ACTGGAGCGA GCCCGAGGTG CCACGGAGCC CTGCGCTGAG CGGCGGGAGG 
CGCCACAGCA GCTCTGGAAG CCTGGCATGG ATGCCCGACG ACGATGTCGA GGCGGACGTC 
GTTCCGGAAC CGGTGGCTGG AGTGCAGCCG GCTGCTGAGA AGCGCAACAC CTTGGACGTG 
GGCGAGGTGC TTAGCAAGAA CGATGCCCTC TCAGACCTGG AGCGCTGGGA GCGCAGCAAG 
AGCAAGAACC GCACGCTGGA CAACAGCGAC CTGCAGCGGC TGGAGCGCGC GCGGGCCGCG 
GCTGCCGGCC CTGGGGCCGC CTCGGAGCAC CGGCTGCTGC GTTTCTTCAG TGGCATCTTC 
GCGCGCAGGG ACGGCGGGCC CTCACCCCGC AGCGGCGCCC TACGCTCGCG CGGTTACTTC 
AGCCTGCGGC GGGCCCCGGC CGACACGCAT TCGTCCAGTG CCGAGAGCAT CGAGGGGTCC 
CCGCGCAGAG ATGCTTTCGT GAACAGCCAG GAATGGACAC TCAGCCGATC AGTACCAGAG 
CTCAAAGTGG GAATTGTGGG TAACCTGGCC AGTGGCAAGT CTGCGCTGGT GCACCGGTAC 
CTGACAGGCA CATACGTGCA GGAGGAGTCT CCGGAAGATA TGGACGCAGG TGGCAGGTTC 
AAGAAGGAGA TTGTTGTTGA TGGACAGAGT TATCTGCTGC TGATTAGGGA TGAAGGGGGT 
CCCCCGGAGG CACAGTTTGC CATGTGGGTG GACGCGGTCA TCTTTGTCTT CAGCTTGGAG 
GATGAGATCA GTTTCCAGAC CGTCTACCAT TACTACAGCC GAATGGCCAA CTACAGGAAC 
ACCAGTGAGA TCCCATTAGT GCTGGTGGGG ACCCAGGACG CCATAAGTTC CACCAACCCG 
AGGGTCATCG ATGACGTCAG GGCTCGGAAG CTCTCCAATG ACCTGAAGCG GTGCACGTAC 
TATGAGACGT GCGCCACCTA TGGGCTCAAC GTGGAGCGGG TCTTCCAGGA CGTTGCCCAG 
AAGATTGTTG CCACGAGGAA GAAGCAGCAG CTGTCCATAG GCCCCTGCAA GTCCTTACCC 
AACTCTCCAA GCCACTCCTC TGTGTGTTCT GCCCAAGTGT CCGCAGTACA CATCAGCCAG 
ACAAGTAACG GAGGTGGGAG TTTAAGCGAC TACTCATCCT CTGTCCCTTC GACCCCAAGC 
ACCAGCCAGA AGGAGCTTCG GATCGACGTC CCTCCCACTG CCAACACTCC AACGCCCGTC 
CGGAAGCAAT CCAAGCGCAG GTCCAACCTA TTCACATCTA GGAAAGGGAG CGACCCAGAC 
AAAGAGAAGA AAGGCCTGGA GAGCCGCGCG GACAGCATCG GGAGCGGGCG AGCCATCCCA 
ATTAAACAGG GCATGCTCCT GAAAAGAAGT GGCAAGTCAC TAAACAAAGA GTGGAAAAAG 
AAGTACGTCA CCCTATGCGA CAATGGTGTG CTGACCTACC ACCCCAGTTT ACATGACTAC 
ATGCAGAACG TCCATGGCAA AGAGATAGAC CTCTTGAGAA CCACTGTGAA GGTGCCGGGG 
AAGAGGCCGC CCCGAGCCAC ATCAGCCTGT GCGCCGATCT CCAGTCCCAA AACCAATGGC 
CTGGCCAAGG ACATGAGCAG TTTACACATC TCACCAAATT CAGGGAATGT CACTAGTGCG 
TCTGGGTCTC AGATGGCAAG CGGCATCAGC CTGGTCTCCT TCAACAGCCG ACCCGATGGC 
ATGCACCAGC GCTCCTACTC AGTCTCCAGT GCCGACCAGT GGAGTGACGC TACAGTCATT 
GCAAACTCAG CCATCAGCAG TGACACGGGG CTGGGTGACT CGGTGTGTTC CAGCCCAAGT 
ATCTCCAGCA GCACCAGCCC CAAGCTCGAC CCACCCCCCT CCCCCCACGC CAACAGAAAG 
AAGCATCGGA GGAAGAAAAG TACCAGCAAC TTCAAGGCCG ACGGGCTCAC GGGCACCGCT 
GAAGGTTCAC ATTCACACTC TGGCCTCAGA CTGAGAGACC CAGTTGCCTG GCGCCCTGGA 
GATCTCCATC CACAGTTCCA TACATCTTTA GGCCAGAAAA GAGCCCCACG GCCCCATCTT 
GGGAGCTTCT GGGTTTCTGC CAAACGCAAA GCATGGAAAC TAAACCGTGT TGGTAGCCTG 
CGAAACATAT ACAGCAGCAG CAGCACCAAC ACCGAAGAGC AAGAAGAAAA CTTGGAGTTT 
ATCATTGTGT CCCTCACTGG CCAGACGTGG CACTTTGAAG CCACCACATA CGAGGAGCGA 
GACGCATGGG TCCAAGCCAT CGAGAGCCAG ATCCTAGCCA GCCTGCAGTC CTGTGAGAGC 
AGCAAGAATA AGTCCCGACT CACCAGCCAG AGCGAAGCCA TGGCACTGCA GTCAATCCGT 
AACATGAGAG GGAACTCGCA CTGCGTGGAC TGTGACACCC AGAACCCCAA CTGGGCCAGT 
TTGAACTTGG GAGCCCTCAT GTGTATCGAG TGCTCAGGGA TCCACCGGAA TCTCGGCACC 
CACCTCTCTC GGGTCCGATC GCTCGACCTC GACGACTGGC CCATGGAGCT GATCAAGGTG 
ATGTCCTCCA TCGGGAATGA GCTGGCCAAC AGCGTGTGGG AAGAGGGCAG CCAGGGACGG 
ACAAAGCCCT CGTTGGACTC CACCAGGGAA GAAAAGGAAC GGTGGATTCG GGCCAAGTAT 
GAGCAGAAAC TCTTCCTGGC CCCACTGCCA TGCACAGAGT TCTCCCTGGG CCAGCAACTG 
CTACATGCCA CTGCTGAGGA AGACCTGCGG ACTGTCATCC TGCTTCTGGC ACACGGGTCA 
CGGGATGAGG TGAATGAGAC CTGTGGGGAA GGAGATGGTC GCACGGCCCT GCACCTGGCC 
TGCCGCAAGG GCAACGTGGT CCTGGTTCAG CTGCTGATCT GGTACGGGGT GGACGTCATG 
GCCCGAGACG CCCACGGGAA CACAGCACTG GCCTATGCCC GTCAGGCCTC CAGCCAGGAG 
TGTATCGACG TGCTTCTACA GTATGGCTGC CCCGACGAGC GCTTTGTGCT CATGGCTACC 
CCCAACCTGT CTAGAAAGAA CAACAGCAGG AACAACAGTA GCGGCAGGGC GCCCAGTGTC 
ATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017451979.1
CDS	2466..6731
Translation	MTLQQAGPGERSIGLLREADPLRAVGIHPSAWACPPPRSPPPPRSRDPQAPLGARPAVSARRPPPARPAPARISILNRRPPAPRPTAAGATTRAARNLGRGGGSRTAELCRKRPWDALRPGALDNEAAGAAARRGLCAPRPHLLARRVPCLSAHPAPHGSALRVRTPAVGPRGTRTGGLQGRCSRSLSAGLAARGGRGRRSPPARALHLASSAPEARVPAAGGAGCSRASEGGEAAAAPGDMYLLDGSGEPASSPADTMSRGMPPRKTVYRISVTMVRKEQLAAPAPRPTRRPRPARSPEAPGRLEEEAEEAAAEDPEIGPRAWDFRAFRTRSTGQLELGRLRPCAHDTEPTDLMGSPTTEGPSSPDGEEARAAPLRRSLSFRHWSEPEVPRSPALSGGRRHSSSGSLAWMPDDDVEADVVPEPVAGVQPAAEKRNTLDVGEVLSKNDALSDLERWERSKSKNRTLDNSDLQRLERARAAAAGPGAASEHRLLRFFSGIFARRDGGPSPRSGALRSRGYFSLRRAPADTHSSSAESIEGSPRRDAFVNSQEWTLSRSVPELKVGIVGNLASGKSALVHRYLTGTYVQEESPEDMDAGGRFKKEIVVDGQSYLLLIRDEGGPPEAQFAMWVDAVIFVFSLEDEISFQTVYHYYSRMANYRNTSEIPLVLVGTQDAISSTNPRVIDDVRARKLSNDLKRCTYYETCATYGLNVERVFQDVAQKIVATRKKQQLSIGPCKSLPNSPSHSSVCSAQVSAVHISQTSNGGGSLSDYSSSVPSTPSTSQKELRIDVPPTANTPTPVRKQSKRRSNLFTSRKGSDPDKEKKGLESRADSIGSGRAIPIKQGMLLKRSGKSLNKEWKKKYVTLCDNGVLTYHPSLHDYMQNVHGKEIDLLRTTVKVPGKRPPRATSACAPISSPKTNGLAKDMSSLHISPNSGNVTSASGSQMASGISLVSFNSRPDGMHQRSYSVSSADQWSDATVIANSAISSDTGLGDSVCSSPSISSSTSPKLDPPPSPHANRKKHRRKKSTSNFKADGLTGTAEGSHSHSGLRLRDPVAWRPGDLHPQFHTSLGQKRAPRPHLGSFWVSAKRKAWKLNRVGSLRNIYSSSSTNTEEQEENLEFIIVSLTGQTWHFEATTYEERDAWVQAIESQILASLQSCESSKNKSRLTSQSEAMALQSIRNMRGNSHCVDCDTQNPNWASLNLGALMCIECSGIHRNLGTHLSRVRSLDLDDWPMELIKVMSSIGNELANSVWEEGSQGRTKPSLDSTREEKERWIRAKYEQKLFLAPLPCTEFSLGQQLLHATAEEDLRTVILLLAHGSRDEVNETCGEGDGRTALHLACRKGNVVLVQLLIWYGVDVMARDAHGNTALAYARQASSQECIDVLLQYGCPDERFVLMATPNLSRKNNSRNNSSGRAPSVI

Target ORF information:

RefSeq Version	XM_017596490.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ArfGAP with GTPase domain, ankyrin repeat and PH domain 1 (Agap1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017596490.1

ORF Insert Sequence:

ATGACCTTGC AGCAAGCAGG TCCTGGAGAG CGCAGTATAG GATTACTGCG CGAAGCTGAT 
CCGCTCCGCG CGGTGGGGAT CCATCCCAGC GCTTGGGCTT GCCCTCCGCC GCGCTCGCCG 
CCGCCTCCGC GCTCCCGGGA CCCGCAGGCC CCGCTGGGCG CGCGGCCCGC AGTCTCCGCA 
CGTCGGCCTC CGCCCGCCCG CCCCGCCCCG GCGCGCATTT CCATTTTAAA CCGCCGCCCG 
CCCGCCCCTC GACCGACCGC GGCCGGCGCA ACCACCCGCG CGGCACGGAA CCTCGGGAGA 
GGCGGAGGCT CCCGGACTGC CGAGCTGTGC AGGAAAAGGC CCTGGGATGC GCTTCGGCCC 
GGCGCACTAG ACAATGAAGC CGCGGGCGCT GCTGCCCGCC GCGGGCTTTG CGCACCCAGG 
CCGCACCTGC TGGCGAGGCG GGTTCCGTGC CTGAGCGCCC ACCCAGCCCC TCATGGCTCG 
GCTCTCCGGG TGCGGACCCC GGCAGTCGGG CCGCGTGGCA CGCGGACGGG CGGACTCCAG 
GGGCGCTGTT CCCGCAGCCT GAGCGCGGGC CTCGCTGCGC GCGGGGGGCG TGGGCGCCGC 
TCCCCGCCCG CTCGGGCTCT GCACCTGGCC AGCTCTGCGC CTGAGGCGCG CGTCCCGGCA 
GCCGGCGGCG CTGGATGCAG CCGGGCCTCG GAGGGCGGCG AGGCGGCCGC GGCCCCTGGG 
GACATGTACC TGCTGGACGG CAGCGGCGAG CCCGCGTCTT CGCCTGCGGA CACGATGTCG 
CGCGGGATGC CCCCCAGGAA GACGGTCTAC CGCATCTCGG TGACCATGGT CAGGAAGGAG 
CAGCTGGCCG CTCCGGCTCC ACGTCCTACT CGGCGGCCCC GGCCCGCGCG CTCCCCGGAG 
GCGCCCGGGC GGCTGGAGGA GGAGGCCGAG GAGGCGGCGG CTGAGGATCC TGAGATCGGG 
CCGCGCGCCT GGGACTTCCG CGCCTTTCGC ACACGCAGCA CCGGGCAGCT GGAGCTCGGG 
CGGCTGCGGC CGTGCGCACA CGACACAGAA CCCACCGACC TGATGGGTAG CCCCACGACT 
GAGGGGCCCA GCAGTCCTGA TGGGGAGGAG GCACGGGCCG CGCCCCTGCG GCGGAGCCTC 
AGCTTTAGGC ACTGGAGCGA GCCCGAGGTG CCACGGAGCC CTGCGCTGAG CGGCGGGAGG 
CGCCACAGCA GCTCTGGAAG CCTGGCATGG ATGCCCGACG ACGATGTCGA GGCGGACGTC 
GTTCCGGAAC CGGTGGCTGG AGTGCAGCCG GCTGCTGAGA AGCGCAACAC CTTGGACGTG 
GGCGAGGTGC TTAGCAAGAA CGATGCCCTC TCAGACCTGG AGCGCTGGGA GCGCAGCAAG 
AGCAAGAACC GCACGCTGGA CAACAGCGAC CTGCAGCGGC TGGAGCGCGC GCGGGCCGCG 
GCTGCCGGCC CTGGGGCCGC CTCGGAGCAC CGGCTGCTGC GTTTCTTCAG TGGCATCTTC 
GCGCGCAGGG ACGGCGGGCC CTCACCCCGC AGCGGCGCCC TACGCTCGCG CGGTTACTTC 
AGCCTGCGGC GGGCCCCGGC CGACACGCAT TCGTCCAGTG CCGAGAGCAT CGAGGGGTCC 
CCGCGCAGAG ATGCTTTCGT GAACAGCCAG GAATGGACAC TCAGCCGATC AGTACCAGAG 
CTCAAAGTGG GAATTGTGGG TAACCTGGCC AGTGGCAAGT CTGCGCTGGT GCACCGGTAC 
CTGACAGGCA CATACGTGCA GGAGGAGTCT CCGGAAGATA TGGACGCAGG TGGCAGGTTC 
AAGAAGGAGA TTGTTGTTGA TGGACAGAGT TATCTGCTGC TGATTAGGGA TGAAGGGGGT 
CCCCCGGAGG CACAGTTTGC CATGTGGGTG GACGCGGTCA TCTTTGTCTT CAGCTTGGAG 
GATGAGATCA GTTTCCAGAC CGTCTACCAT TACTACAGCC GAATGGCCAA CTACAGGAAC 
ACCAGTGAGA TCCCATTAGT GCTGGTGGGG ACCCAGGACG CCATAAGTTC CACCAACCCG 
AGGGTCATCG ATGACGTCAG GGCTCGGAAG CTCTCCAATG ACCTGAAGCG GTGCACGTAC 
TATGAGACGT GCGCCACCTA TGGGCTCAAC GTGGAGCGGG TCTTCCAGGA CGTTGCCCAG 
AAGATTGTTG CCACGAGGAA GAAGCAGCAG CTGTCCATAG GCCCCTGCAA GTCCTTACCC 
AACTCTCCAA GCCACTCCTC TGTGTGTTCT GCCCAAGTGT CCGCAGTACA CATCAGCCAG 
ACAAGTAACG GAGGTGGGAG TTTAAGCGAC TACTCATCCT CTGTCCCTTC GACCCCAAGC 
ACCAGCCAGA AGGAGCTTCG GATCGACGTC CCTCCCACTG CCAACACTCC AACGCCCGTC 
CGGAAGCAAT CCAAGCGCAG GTCCAACCTA TTCACATCTA GGAAAGGGAG CGACCCAGAC 
AAAGAGAAGA AAGGCCTGGA GAGCCGCGCG GACAGCATCG GGAGCGGGCG AGCCATCCCA 
ATTAAACAGG GCATGCTCCT GAAAAGAAGT GGCAAGTCAC TAAACAAAGA GTGGAAAAAG 
AAGTACGTCA CCCTATGCGA CAATGGTGTG CTGACCTACC ACCCCAGTTT ACATGACTAC 
ATGCAGAACG TCCATGGCAA AGAGATAGAC CTCTTGAGAA CCACTGTGAA GGTGCCGGGG 
AAGAGGCCGC CCCGAGCCAC ATCAGCCTGT GCGCCGATCT CCAGTCCCAA AACCAATGGC 
CTGGCCAAGG ACATGAGCAG TTTACACATC TCACCAAATT CAGGGAATGT CACTAGTGCG 
TCTGGGTCTC AGATGGCAAG CGGCATCAGC CTGGTCTCCT TCAACAGCCG ACCCGATGGC 
ATGCACCAGC GCTCCTACTC AGTCTCCAGT GCCGACCAGT GGAGTGACGC TACAGTCATT 
GCAAACTCAG CCATCAGCAG TGACACGGGG CTGGGTGACT CGGTGTGTTC CAGCCCAAGT 
ATCTCCAGCA GCACCAGCCC CAAGCTCGAC CCACCCCCCT CCCCCCACGC CAACAGAAAG 
AAGCATCGGA GGAAGAAAAG TACCAGCAAC TTCAAGGCCG ACGGGCTCAC GGGCACCGCT 
GAAGGTTCAC ATTCACACTC TGGCCTCAGA CTGAGAGACC CAGTTGCCTG GCGCCCTGGA 
GATCTCCATC CACAGTTCCA TACATCTTTA GGCCAGAAAA GAGCCCCACG GCCCCATCTT 
GGGAGCTTCT GGGTTTCTGC CAAACGCAAA GCATGGAAAC TAAACCGTGT TGGTAGCCTG 
CGAAACATAT ACAGCAGCAG CAGCACCAAC ACCGAAGAGC AAGAAGAAAA CTTGGAGTTT 
ATCATTGTGT CCCTCACTGG CCAGACGTGG CACTTTGAAG CCACCACATA CGAGGAGCGA 
GACGCATGGG TCCAAGCCAT CGAGAGCCAG ATCCTAGCCA GCCTGCAGTC CTGTGAGAGC 
AGCAAGAATA AGTCCCGACT CACCAGCCAG AGCGAAGCCA TGGCACTGCA GTCAATCCGT 
AACATGAGAG GGAACTCGCA CTGCGTGGAC TGTGACACCC AGAACCCCAA CTGGGCCAGT 
TTGAACTTGG GAGCCCTCAT GTGTATCGAG TGCTCAGGGA TCCACCGGAA TCTCGGCACC 
CACCTCTCTC GGGTCCGATC GCTCGACCTC GACGACTGGC CCATGGAGCT GATCAAGGTG 
ATGTCCTCCA TCGGGAATGA GCTGGCCAAC AGCGTGTGGG AAGAGGGCAG CCAGGGACGG 
ACAAAGCCCT CGTTGGACTC CACCAGGGAA GAAAAGGAAC GGTGGATTCG GGCCAAGTAT 
GAGCAGAAAC TCTTCCTGGC CCCACTGCCA TGCACAGAGT TCTCCCTGGG CCAGCAACTG 
CTACATGCCA CTGCTGAGGA AGACCTGCGG ACTGTCATCC TGCTTCTGGC ACACGGGTCA 
CGGGATGAGG TGAATGAGAC CTGTGGGGAA GGAGATGGTC GCACGGCCCT GCACCTGGCC 
TGCCGCAAGG GCAACGTGGT CCTGGTTCAG CTGCTGATCT GGTACGGGGT GGACGTCATG 
GCCCGAGACG CCCACGGGAA CACAGCACTG GCCTATGCCC GTCAGGCCTC CAGCCAGGAG 
TGTATCGACG TGCTTCTACA GTATGGCTGC CCCGACGAGC GCTTTGTGCT CATGGCTACC 
CCCAACCTGT CTAGAAAGAA CAACAGCAGG AACAACAGTA GCGGCAGGGC GCCCAGTGTC 
ATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Agap1 ( XM_017596491.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017451980.1 Rattus norvegicus ArfGAP with GTPase domain, ankyrin repeat and PH domain 1 (Agap1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa62784
Clone ID Related Accession (Same CDS sequence)	XM_017596491.1
Accession Version	XM_017596491.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4254bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	arf-GAP with GTPase, ANK repeat and PH domain-containing protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005108.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCTTGC AGCAAGCAGG TCCTGGAGAG CGCAGTATAG GATTACTGCG CGAAGCTGAT 
CCGCTCCGCG CGGTGGGGAT CCATCCCAGC GCTTGGGCTT GCCCTCCGCC GCGCTCGCCG 
CCGCCTCCGC GCTCCCGGGA CCCGCAGGCC CCGCTGGGCG CGCGGCCCGC AGTCTCCGCA 
CGTCGGCCTC CGCCCGCCCG CCCCGCCCCG GCGCGCATTT CCATTTTAAA CCGCCGCCCG 
CCCGCCCCTC GACCGACCGC GGCCGGCGCA ACCACCCGCG CGGCACGGAA CCTCGGGAGA 
GGCGGAGGCT CCCGGACTGC CGAGCTGTGC AGGAAAAGGC CCTGGGATGC GCTTCGGCCC 
GGCGCACTAG ACAATGAAGC CGCGGGCGCT GCTGCCCGCC GCGGGCTTTG CGCACCCAGG 
CCGCACCTGC TGGCGAGGCG GGTTCCGTGC CTGAGCGCCC ACCCAGCCCC TCATGGCTCG 
GCTCTCCGGG TGCGGACCCC GGCAGTCGGG CCGCGTGGCA CGCGGACGGG CGGACTCCAG 
GGGCGCTGTT CCCGCAGCCT GAGCGCGGGC CTCGCTGCGC GCGGGGGGCG TGGGCGCCGC 
TCCCCGCCCG CTCGGGCTCT GCACCTGGCC AGCTCTGCGC CTGAGGCGCG CGTCCCGGCA 
GCCGGCGGCG CTGGATGCAG CCGGGCCTCG GAGGGCGGCG AGGCGGCCGC GGCCCCTGGG 
GACATGTACC TGCTGGACGG CAGCGGCGAG CCCGCGTCTT CGCCTGCGGA CACGATGTCG 
CGCGGGATGC CCCCCAGGAA GACGGTCTAC CGCATCTCGG TGACCATGGT CAGGAAGGAG 
CAGCTGGCCG CTCCGGCTCC ACGTCCTACT CGGCGGCCCC GGCCCGCGCG CTCCCCGGAG 
GCGCCCGGGC GGCTGGAGGA GGAGGCCGAG GAGGCGGCGG CTGAGGATCC TGAGATCGGG 
CCGCGCGCCT GGGACTTCCG CGCCTTTCGC ACACGCAGCA CCGGGCAGCT GGAGCTCGGG 
CGGCTGCGGC CGTGCGCACA CGACACAGAA CCCACCGACC TGATGGGTAG CCCCACGACT 
GAGGGGCCCA GCAGTCCTGA TGGGGAGGAG GCACGGGCCG CGCCCCTGCG GCGGAGCCTC 
AGCTTTAGGC ACTGGAGCGA GCCCGAGGTG CCACGGAGCC CTGCGCTGAG CGGCGGGAGG 
CGCCACAGCA GCTCTGGAAG CCTGGCATGG ATGCCCGACG ACGATGTCGA GGCGGACGTC 
GTTCCGGAAC CGGTGGCTGG AGTGCAGCCG GCTGCTGAGA AGCGCAACAC CTTGGACGTG 
GGCGAGGTGC TTAGCAAGAA CGATGCCCTC TCAGACCTGG AGCGCTGGGA GCGCAGCAAG 
AGCAAGAACC GCACGCTGGA CAACAGCGAC CTGCAGCGGC TGGAGCGCGC GCGGGCCGCG 
GCTGCCGGCC CTGGGGCCGC CTCGGAGCAC CGGCTGCTGC GTTTCTTCAG TGGCATCTTC 
GCGCGCAGGG ACGGCGGGCC CTCACCCCGC AGCGGCGCCC TACGCTCGCG CGGTTACTTC 
AGCCTGCGGC GGGCCCCGGC CGACACGCAT TCGTCCAGTG CCGAGAGCAT CGAGGGGTCC 
CCGCGCAGAG ATGCTTTCGT GAACAGCCAG GAATGGACAC TCAGCCGATC AGTACCAGAG 
CTCAAAGTGG GAATTGTGGG TAACCTGGCC AGTGGCAAGT CTGCGCTGGT GCACCGGTAC 
CTGACAGGCA CATACGTGCA GGAGGAGTCT CCGGAAGGTG GCAGGTTCAA GAAGGAGATT 
GTTGTTGATG GACAGAGTTA TCTGCTGCTG ATTAGGGATG AAGGGGGTCC CCCGGAGGCA 
CAGTTTGCCA TGTGGGTGGA CGCGGTCATC TTTGTCTTCA GCTTGGAGGA TGAGATCAGT 
TTCCAGACCG TCTACCATTA CTACAGCCGA ATGGCCAACT ACAGGAACAC CAGTGAGATC 
CCATTAGTGC TGGTGGGGAC CCAGGACGCC ATAAGTTCCA CCAACCCGAG GGTCATCGAT 
GACGTCAGGG CTCGGAAGCT CTCCAATGAC CTGAAGCGGT GCACGTACTA TGAGACGTGC 
GCCACCTATG GGCTCAACGT GGAGCGGGTC TTCCAGGACG TTGCCCAGAA GATTGTTGCC 
ACGAGGAAGA AGCAGCAGCT GTCCATAGGC CCCTGCAAGT CCTTACCCAA CTCTCCAAGC 
CACTCCTCTG TGTGTTCTGC CCAAGTGTCC GCAGTACACA TCAGCCAGAC AAGTAACGGA 
GGTGGGAGTT TAAGCGACTA CTCATCCTCT GTCCCTTCGA CCCCAAGCAC CAGCCAGAAG 
GAGCTTCGGA TCGACGTCCC TCCCACTGCC AACACTCCAA CGCCCGTCCG GAAGCAATCC 
AAGCGCAGGT CCAACCTATT CACATCTAGG AAAGGGAGCG ACCCAGACAA AGAGAAGAAA 
GGCCTGGAGA GCCGCGCGGA CAGCATCGGG AGCGGGCGAG CCATCCCAAT TAAACAGGGC 
ATGCTCCTGA AAAGAAGTGG CAAGTCACTA AACAAAGAGT GGAAAAAGAA GTACGTCACC 
CTATGCGACA ATGGTGTGCT GACCTACCAC CCCAGTTTAC ATGACTACAT GCAGAACGTC 
CATGGCAAAG AGATAGACCT CTTGAGAACC ACTGTGAAGG TGCCGGGGAA GAGGCCGCCC 
CGAGCCACAT CAGCCTGTGC GCCGATCTCC AGTCCCAAAA CCAATGGCCT GGCCAAGGAC 
ATGAGCAGTT TACACATCTC ACCAAATTCA GGGAATGTCA CTAGTGCGTC TGGGTCTCAG 
ATGGCAAGCG GCATCAGCCT GGTCTCCTTC AACAGCCGAC CCGATGGCAT GCACCAGCGC 
TCCTACTCAG TCTCCAGTGC CGACCAGTGG AGTGACGCTA CAGTCATTGC AAACTCAGCC 
ATCAGCAGTG ACACGGGGCT GGGTGACTCG GTGTGTTCCA GCCCAAGTAT CTCCAGCAGC 
ACCAGCCCCA AGCTCGACCC ACCCCCCTCC CCCCACGCCA ACAGAAAGAA GCATCGGAGG 
AAGAAAAGTA CCAGCAACTT CAAGGCCGAC GGGCTCACGG GCACCGCTGA AGGTTCACAT 
TCACACTCTG GCCTCAGACT GAGAGACCCA GTTGCCTGGC GCCCTGGAGA TCTCCATCCA 
CAGTTCCATA CATCTTTAGG CCAGAAAAGA GCCCCACGGC CCCATCTTGG GAGCTTCTGG 
GTTTCTGCCA AACGCAAAGC ATGGAAACTA AACCGTGTTG GTAGCCTGCG AAACATATAC 
AGCAGCAGCA GCACCAACAC CGAAGAGCAA GAAGAAAACT TGGAGTTTAT CATTGTGTCC 
CTCACTGGCC AGACGTGGCA CTTTGAAGCC ACCACATACG AGGAGCGAGA CGCATGGGTC 
CAAGCCATCG AGAGCCAGAT CCTAGCCAGC CTGCAGTCCT GTGAGAGCAG CAAGAATAAG 
TCCCGACTCA CCAGCCAGAG CGAAGCCATG GCACTGCAGT CAATCCGTAA CATGAGAGGG 
AACTCGCACT GCGTGGACTG TGACACCCAG AACCCCAACT GGGCCAGTTT GAACTTGGGA 
GCCCTCATGT GTATCGAGTG CTCAGGGATC CACCGGAATC TCGGCACCCA CCTCTCTCGG 
GTCCGATCGC TCGACCTCGA CGACTGGCCC ATGGAGCTGA TCAAGGTGAT GTCCTCCATC 
GGGAATGAGC TGGCCAACAG CGTGTGGGAA GAGGGCAGCC AGGGACGGAC AAAGCCCTCG 
TTGGACTCCA CCAGGGAAGA AAAGGAACGG TGGATTCGGG CCAAGTATGA GCAGAAACTC 
TTCCTGGCCC CACTGCCATG CACAGAGTTC TCCCTGGGCC AGCAACTGCT ACATGCCACT 
GCTGAGGAAG ACCTGCGGAC TGTCATCCTG CTTCTGGCAC ACGGGTCACG GGATGAGGTG 
AATGAGACCT GTGGGGAAGG AGATGGTCGC ACGGCCCTGC ACCTGGCCTG CCGCAAGGGC 
AACGTGGTCC TGGTTCAGCT GCTGATCTGG TACGGGGTGG ACGTCATGGC CCGAGACGCC 
CACGGGAACA CAGCACTGGC CTATGCCCGT CAGGCCTCCA GCCAGGAGTG TATCGACGTG 
CTTCTACAGT ATGGCTGCCC CGACGAGCGC TTTGTGCTCA TGGCTACCCC CAACCTGTCT 
AGAAAGAACA ACAGCAGGAA CAACAGTAGC GGCAGGGCGC CCAGTGTCAT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017451980.1
CDS	2465..6718
Translation	MTLQQAGPGERSIGLLREADPLRAVGIHPSAWACPPPRSPPPPRSRDPQAPLGARPAVSARRPPPARPAPARISILNRRPPAPRPTAAGATTRAARNLGRGGGSRTAELCRKRPWDALRPGALDNEAAGAAARRGLCAPRPHLLARRVPCLSAHPAPHGSALRVRTPAVGPRGTRTGGLQGRCSRSLSAGLAARGGRGRRSPPARALHLASSAPEARVPAAGGAGCSRASEGGEAAAAPGDMYLLDGSGEPASSPADTMSRGMPPRKTVYRISVTMVRKEQLAAPAPRPTRRPRPARSPEAPGRLEEEAEEAAAEDPEIGPRAWDFRAFRTRSTGQLELGRLRPCAHDTEPTDLMGSPTTEGPSSPDGEEARAAPLRRSLSFRHWSEPEVPRSPALSGGRRHSSSGSLAWMPDDDVEADVVPEPVAGVQPAAEKRNTLDVGEVLSKNDALSDLERWERSKSKNRTLDNSDLQRLERARAAAAGPGAASEHRLLRFFSGIFARRDGGPSPRSGALRSRGYFSLRRAPADTHSSSAESIEGSPRRDAFVNSQEWTLSRSVPELKVGIVGNLASGKSALVHRYLTGTYVQEESPEGGRFKKEIVVDGQSYLLLIRDEGGPPEAQFAMWVDAVIFVFSLEDEISFQTVYHYYSRMANYRNTSEIPLVLVGTQDAISSTNPRVIDDVRARKLSNDLKRCTYYETCATYGLNVERVFQDVAQKIVATRKKQQLSIGPCKSLPNSPSHSSVCSAQVSAVHISQTSNGGGSLSDYSSSVPSTPSTSQKELRIDVPPTANTPTPVRKQSKRRSNLFTSRKGSDPDKEKKGLESRADSIGSGRAIPIKQGMLLKRSGKSLNKEWKKKYVTLCDNGVLTYHPSLHDYMQNVHGKEIDLLRTTVKVPGKRPPRATSACAPISSPKTNGLAKDMSSLHISPNSGNVTSASGSQMASGISLVSFNSRPDGMHQRSYSVSSADQWSDATVIANSAISSDTGLGDSVCSSPSISSSTSPKLDPPPSPHANRKKHRRKKSTSNFKADGLTGTAEGSHSHSGLRLRDPVAWRPGDLHPQFHTSLGQKRAPRPHLGSFWVSAKRKAWKLNRVGSLRNIYSSSSTNTEEQEENLEFIIVSLTGQTWHFEATTYEERDAWVQAIESQILASLQSCESSKNKSRLTSQSEAMALQSIRNMRGNSHCVDCDTQNPNWASLNLGALMCIECSGIHRNLGTHLSRVRSLDLDDWPMELIKVMSSIGNELANSVWEEGSQGRTKPSLDSTREEKERWIRAKYEQKLFLAPLPCTEFSLGQQLLHATAEEDLRTVILLLAHGSRDEVNETCGEGDGRTALHLACRKGNVVLVQLLIWYGVDVMARDAHGNTALAYARQASSQECIDVLLQYGCPDERFVLMATPNLSRKNNSRNNSSGRAPSVI

Target ORF information:

RefSeq Version	XM_017596491.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ArfGAP with GTPase domain, ankyrin repeat and PH domain 1 (Agap1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017596491.1

ORF Insert Sequence:

ATGACCTTGC AGCAAGCAGG TCCTGGAGAG CGCAGTATAG GATTACTGCG CGAAGCTGAT 
CCGCTCCGCG CGGTGGGGAT CCATCCCAGC GCTTGGGCTT GCCCTCCGCC GCGCTCGCCG 
CCGCCTCCGC GCTCCCGGGA CCCGCAGGCC CCGCTGGGCG CGCGGCCCGC AGTCTCCGCA 
CGTCGGCCTC CGCCCGCCCG CCCCGCCCCG GCGCGCATTT CCATTTTAAA CCGCCGCCCG 
CCCGCCCCTC GACCGACCGC GGCCGGCGCA ACCACCCGCG CGGCACGGAA CCTCGGGAGA 
GGCGGAGGCT CCCGGACTGC CGAGCTGTGC AGGAAAAGGC CCTGGGATGC GCTTCGGCCC 
GGCGCACTAG ACAATGAAGC CGCGGGCGCT GCTGCCCGCC GCGGGCTTTG CGCACCCAGG 
CCGCACCTGC TGGCGAGGCG GGTTCCGTGC CTGAGCGCCC ACCCAGCCCC TCATGGCTCG 
GCTCTCCGGG TGCGGACCCC GGCAGTCGGG CCGCGTGGCA CGCGGACGGG CGGACTCCAG 
GGGCGCTGTT CCCGCAGCCT GAGCGCGGGC CTCGCTGCGC GCGGGGGGCG TGGGCGCCGC 
TCCCCGCCCG CTCGGGCTCT GCACCTGGCC AGCTCTGCGC CTGAGGCGCG CGTCCCGGCA 
GCCGGCGGCG CTGGATGCAG CCGGGCCTCG GAGGGCGGCG AGGCGGCCGC GGCCCCTGGG 
GACATGTACC TGCTGGACGG CAGCGGCGAG CCCGCGTCTT CGCCTGCGGA CACGATGTCG 
CGCGGGATGC CCCCCAGGAA GACGGTCTAC CGCATCTCGG TGACCATGGT CAGGAAGGAG 
CAGCTGGCCG CTCCGGCTCC ACGTCCTACT CGGCGGCCCC GGCCCGCGCG CTCCCCGGAG 
GCGCCCGGGC GGCTGGAGGA GGAGGCCGAG GAGGCGGCGG CTGAGGATCC TGAGATCGGG 
CCGCGCGCCT GGGACTTCCG CGCCTTTCGC ACACGCAGCA CCGGGCAGCT GGAGCTCGGG 
CGGCTGCGGC CGTGCGCACA CGACACAGAA CCCACCGACC TGATGGGTAG CCCCACGACT 
GAGGGGCCCA GCAGTCCTGA TGGGGAGGAG GCACGGGCCG CGCCCCTGCG GCGGAGCCTC 
AGCTTTAGGC ACTGGAGCGA GCCCGAGGTG CCACGGAGCC CTGCGCTGAG CGGCGGGAGG 
CGCCACAGCA GCTCTGGAAG CCTGGCATGG ATGCCCGACG ACGATGTCGA GGCGGACGTC 
GTTCCGGAAC CGGTGGCTGG AGTGCAGCCG GCTGCTGAGA AGCGCAACAC CTTGGACGTG 
GGCGAGGTGC TTAGCAAGAA CGATGCCCTC TCAGACCTGG AGCGCTGGGA GCGCAGCAAG 
AGCAAGAACC GCACGCTGGA CAACAGCGAC CTGCAGCGGC TGGAGCGCGC GCGGGCCGCG 
GCTGCCGGCC CTGGGGCCGC CTCGGAGCAC CGGCTGCTGC GTTTCTTCAG TGGCATCTTC 
GCGCGCAGGG ACGGCGGGCC CTCACCCCGC AGCGGCGCCC TACGCTCGCG CGGTTACTTC 
AGCCTGCGGC GGGCCCCGGC CGACACGCAT TCGTCCAGTG CCGAGAGCAT CGAGGGGTCC 
CCGCGCAGAG ATGCTTTCGT GAACAGCCAG GAATGGACAC TCAGCCGATC AGTACCAGAG 
CTCAAAGTGG GAATTGTGGG TAACCTGGCC AGTGGCAAGT CTGCGCTGGT GCACCGGTAC 
CTGACAGGCA CATACGTGCA GGAGGAGTCT CCGGAAGGTG GCAGGTTCAA GAAGGAGATT 
GTTGTTGATG GACAGAGTTA TCTGCTGCTG ATTAGGGATG AAGGGGGTCC CCCGGAGGCA 
CAGTTTGCCA TGTGGGTGGA CGCGGTCATC TTTGTCTTCA GCTTGGAGGA TGAGATCAGT 
TTCCAGACCG TCTACCATTA CTACAGCCGA ATGGCCAACT ACAGGAACAC CAGTGAGATC 
CCATTAGTGC TGGTGGGGAC CCAGGACGCC ATAAGTTCCA CCAACCCGAG GGTCATCGAT 
GACGTCAGGG CTCGGAAGCT CTCCAATGAC CTGAAGCGGT GCACGTACTA TGAGACGTGC 
GCCACCTATG GGCTCAACGT GGAGCGGGTC TTCCAGGACG TTGCCCAGAA GATTGTTGCC 
ACGAGGAAGA AGCAGCAGCT GTCCATAGGC CCCTGCAAGT CCTTACCCAA CTCTCCAAGC 
CACTCCTCTG TGTGTTCTGC CCAAGTGTCC GCAGTACACA TCAGCCAGAC AAGTAACGGA 
GGTGGGAGTT TAAGCGACTA CTCATCCTCT GTCCCTTCGA CCCCAAGCAC CAGCCAGAAG 
GAGCTTCGGA TCGACGTCCC TCCCACTGCC AACACTCCAA CGCCCGTCCG GAAGCAATCC 
AAGCGCAGGT CCAACCTATT CACATCTAGG AAAGGGAGCG ACCCAGACAA AGAGAAGAAA 
GGCCTGGAGA GCCGCGCGGA CAGCATCGGG AGCGGGCGAG CCATCCCAAT TAAACAGGGC 
ATGCTCCTGA AAAGAAGTGG CAAGTCACTA AACAAAGAGT GGAAAAAGAA GTACGTCACC 
CTATGCGACA ATGGTGTGCT GACCTACCAC CCCAGTTTAC ATGACTACAT GCAGAACGTC 
CATGGCAAAG AGATAGACCT CTTGAGAACC ACTGTGAAGG TGCCGGGGAA GAGGCCGCCC 
CGAGCCACAT CAGCCTGTGC GCCGATCTCC AGTCCCAAAA CCAATGGCCT GGCCAAGGAC 
ATGAGCAGTT TACACATCTC ACCAAATTCA GGGAATGTCA CTAGTGCGTC TGGGTCTCAG 
ATGGCAAGCG GCATCAGCCT GGTCTCCTTC AACAGCCGAC CCGATGGCAT GCACCAGCGC 
TCCTACTCAG TCTCCAGTGC CGACCAGTGG AGTGACGCTA CAGTCATTGC AAACTCAGCC 
ATCAGCAGTG ACACGGGGCT GGGTGACTCG GTGTGTTCCA GCCCAAGTAT CTCCAGCAGC 
ACCAGCCCCA AGCTCGACCC ACCCCCCTCC CCCCACGCCA ACAGAAAGAA GCATCGGAGG 
AAGAAAAGTA CCAGCAACTT CAAGGCCGAC GGGCTCACGG GCACCGCTGA AGGTTCACAT 
TCACACTCTG GCCTCAGACT GAGAGACCCA GTTGCCTGGC GCCCTGGAGA TCTCCATCCA 
CAGTTCCATA CATCTTTAGG CCAGAAAAGA GCCCCACGGC CCCATCTTGG GAGCTTCTGG 
GTTTCTGCCA AACGCAAAGC ATGGAAACTA AACCGTGTTG GTAGCCTGCG AAACATATAC 
AGCAGCAGCA GCACCAACAC CGAAGAGCAA GAAGAAAACT TGGAGTTTAT CATTGTGTCC 
CTCACTGGCC AGACGTGGCA CTTTGAAGCC ACCACATACG AGGAGCGAGA CGCATGGGTC 
CAAGCCATCG AGAGCCAGAT CCTAGCCAGC CTGCAGTCCT GTGAGAGCAG CAAGAATAAG 
TCCCGACTCA CCAGCCAGAG CGAAGCCATG GCACTGCAGT CAATCCGTAA CATGAGAGGG 
AACTCGCACT GCGTGGACTG TGACACCCAG AACCCCAACT GGGCCAGTTT GAACTTGGGA 
GCCCTCATGT GTATCGAGTG CTCAGGGATC CACCGGAATC TCGGCACCCA CCTCTCTCGG 
GTCCGATCGC TCGACCTCGA CGACTGGCCC ATGGAGCTGA TCAAGGTGAT GTCCTCCATC 
GGGAATGAGC TGGCCAACAG CGTGTGGGAA GAGGGCAGCC AGGGACGGAC AAAGCCCTCG 
TTGGACTCCA CCAGGGAAGA AAAGGAACGG TGGATTCGGG CCAAGTATGA GCAGAAACTC 
TTCCTGGCCC CACTGCCATG CACAGAGTTC TCCCTGGGCC AGCAACTGCT ACATGCCACT 
GCTGAGGAAG ACCTGCGGAC TGTCATCCTG CTTCTGGCAC ACGGGTCACG GGATGAGGTG 
AATGAGACCT GTGGGGAAGG AGATGGTCGC ACGGCCCTGC ACCTGGCCTG CCGCAAGGGC 
AACGTGGTCC TGGTTCAGCT GCTGATCTGG TACGGGGTGG ACGTCATGGC CCGAGACGCC 
CACGGGAACA CAGCACTGGC CTATGCCCGT CAGGCCTCCA GCCAGGAGTG TATCGACGTG 
CTTCTACAGT ATGGCTGCCC CGACGAGCGC TTTGTGCTCA TGGCTACCCC CAACCTGTCT 
AGAAAGAACA ACAGCAGGAA CAACAGTAGC GGCAGGGCGC CCAGTGTCAT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Agap1 ( XM_017596492.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017451981.1 Rattus norvegicus ArfGAP with GTPase domain, ankyrin repeat and PH domain 1 (Agap1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ORa62785
Clone ID Related Accession (Same CDS sequence)	XM_017596492.1
Accession Version	XM_017596492.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4131bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	arf-GAP with GTPase, ANK repeat and PH domain-containing protein 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005108.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCTTGC AGCAAGCAGG TCCTGGAGAG CGCAGTATAG GATTACTGCG CGAAGCTGAT 
CCGCTCCGCG CGGTGGGGAT CCATCCCAGC GCTTGGGCTT GCCCTCCGCC GCGCTCGCCG 
CCGCCTCCGC GCTCCCGGGA CCCGCAGGCC CCGCTGGGCG CGCGGCCCGC AGTCTCCGCA 
CGTCGGCCTC CGCCCGCCCG CCCCGCCCCG GCGCGCATTT CCATTTTAAA CCGCCGCCCG 
CCCGCCCCTC GACCGACCGC GGCCGGCGCA ACCACCCGCG CGGCACGGAA CCTCGGGAGA 
GGCGGAGGCT CCCGGACTGC CGAGCTGTGC AGGAAAAGGC CCTGGGATGC GCTTCGGCCC 
GGCGCACTAG ACAATGAAGC CGCGGGCGCT GCTGCCCGCC GCGGGCTTTG CGCACCCAGG 
CCGCACCTGC TGGCGAGGCG GGTTCCGTGC CTGAGCGCCC ACCCAGCCCC TCATGGCTCG 
GCTCTCCGGG TGCGGACCCC GGCAGTCGGG CCGCGTGGCA CGCGGACGGG CGGACTCCAG 
GGGCGCTGTT CCCGCAGCCT GAGCGCGGGC CTCGCTGCGC GCGGGGGGCG TGGGCGCCGC 
TCCCCGCCCG CTCGGGCTCT GCACCTGGCC AGCTCTGCGC CTGAGGCGCG CGTCCCGGCA 
GCCGGCGGCG CTGGATGCAG CCGGGCCTCG GAGGGCGGCG AGGCGGCCGC GGCCCCTGGG 
GACATGTACC TGCTGGACGG CAGCGGCGAG CCCGCGTCTT CGCCTGCGGA CACGATGTCG 
CGCGGGATGC CCCCCAGGAA GACGGTCTAC CGCATCTCGG TGACCATGGT CAGGAAGGAG 
CAGCTGGCCG CTCCGGCTCC ACGTCCTACT CGGCGGCCCC GGCCCGCGCG CTCCCCGGAG 
GCGCCCGGGC GGCTGGAGGA GGAGGCCGAG GAGGCGGCGG CTGAGGATCC TGAGATCGGG 
CCGCGCGCCT GGGACTTCCG CGCCTTTCGC ACACGCAGCA CCGGGCAGCT GGAGCTCGGG 
CGGCTGCGGC CGTGCGCACA CGACACAGAA CCCACCGACC TGATGGGTAG CCCCACGACT 
GAGGGGCCCA GCAGTCCTGA TGGGGAGGAG GCACGGGCCG CGCCCCTGCG GCGGAGCCTC 
AGCTTTAGGC ACTGGAGCGA GCCCGAGGTG CCACGGAGCC CTGCGCTGAG CGGCGGGAGG 
CGCCACAGCA GCTCTGGAAG CCTGGCATGG ATGCCCGACG ACGATGTCGA GGCGGACGTC 
GTTCCGGAAC CGGTGGCTGG AGTGCAGCCG GCTGCTGAGA AGCGCAACAC CTTGGACGTG 
GGCGAGGTGC TTAGCAAGAA CGATGCCCTC TCAGACCTGG AGCGCTGGGA GCGCAGCAAG 
AGCAAGAACC GCACGCTGGA CAACAGCGAC CTGCAGCGGC TGGAGCGCGC GCGGGCCGCG 
GCTGCCGGCC CTGGGGCCGC CTCGGAGCAC CGGCTGCTGC GTTTCTTCAG TGGCATCTTC 
GCGCGCAGGG ACGGCGGGCC CTCACCCCGC AGCGGCGCCC TACGCTCGCG CGGTTACTTC 
AGCCTGCGGC GGGCCCCGGC CGACACGCAT TCGTCCAGTG CCGAGAGCAT CGAGGGGTCC 
CCGCGCAGAG ATGCTTTCGT GAACAGCCAG GAATGGACAC TCAGCCGATC AGTACCAGAG 
CTCAAAGTGG GAATTGTGGG TAACCTGGCC AGTGGCAAGT CTGCGCTGGT GCACCGGTAC 
CTGACAGGCA CATACGTGCA GGAGGAGTCT CCGGAAGATA TGGACGCAGG TGGCAGGTTC 
AAGAAGGAGA TTGTTGTTGA TGGACAGAGT TATCTGCTGC TGATTAGGGA TGAAGGGGGT 
CCCCCGGAGG CACAGTTTGC CATGTGGGTG GACGCGGTCA TCTTTGTCTT CAGCTTGGAG 
GATGAGATCA GTTTCCAGAC CGTCTACCAT TACTACAGCC GAATGGCCAA CTACAGGAAC 
ACCAGTGAGA TCCCATTAGT GCTGGTGGGG ACCCAGGACG CCATAAGTTC CACCAACCCG 
AGGGTCATCG ATGACGTCAG GGCTCGGAAG CTCTCCAATG ACCTGAAGCG GTGCACGTAC 
TATGAGACGT GCGCCACCTA TGGGCTCAAC GTGGAGCGGG TCTTCCAGGA CGTTGCCCAG 
AAGATTGTTG CCACGAGGAA GAAGCAGCAG CTGTCCATAG GCCCCTGCAA GTCCTTACCC 
AACTCTCCAA GCCACTCCTC TGTGTGTTCT GCCCAAGTGT CCGCAGTACA CATCAGCCAG 
ACAAGTAACG GAGGTGGGAG TTTAAGCGAC TACTCATCCT CTGTCCCTTC GACCCCAAGC 
ACCAGCCAGA AGGAGCTTCG GATCGACGTC CCTCCCACTG CCAACACTCC AACGCCCGTC 
CGGAAGCAAT CCAAGCGCAG GTCCAACCTA TTCACATCTA GGAAAGGGAG CGACCCAGAC 
AAAGAGAAGA AAGGCCTGGA GAGCCGCGCG GACAGCATCG GGAGCGGGCG AGCCATCCCA 
ATTAAACAGG GCATGCTCCT GAAAAGAAGT GGCAAGTCAC TAAACAAAGA GTGGAAAAAG 
AAGTACGTCA CCCTATGCGA CAATGGTGTG CTGACCTACC ACCCCAGTTT ACATGACTAC 
ATGCAGAACG TCCATGGCAA AGAGATAGAC CTCTTGAGAA CCACTGTGAA GGTGCCGGGG 
AAGAGGCCGC CCCGAGCCAC ATCAGCCTGT GCGCCGATCT CCAGTCCCAA AACCAATGGC 
CTGGCCAAGG ACATGAGCAG TTTACACATC TCACCAAATT CAGGGAATGT CACTAGTGCG 
TCTGGGTCTC AGATGGCAAG CGGCATCAGC CTGGTCTCCT TCAACAGCCG ACCCGATGGC 
ATGCACCAGC GCTCCTACTC AGTCTCCAGT GCCGACCAGT GGAGTGACGC TACAGTCATT 
GCAAACTCAG CCATCAGCAG TGACACGGGG CTGGGTGACT CGGTGTGTTC CAGCCCAAGT 
ATCTCCAGCA GCACCAGCCC CAAGCTCGAC CCACCCCCCT CCCCCCACGC CAACAGAAAG 
AAGCATCGGA GGAAGAAAAG TACCAGCAAC TTCAAGGCCG ACGGGCTCAC GGGCACCGCT 
GAAGCCAAAC GCAAAGCATG GAAACTAAAC CGTGTTGGTA GCCTGCGAAA CATATACAGC 
AGCAGCAGCA CCAACACCGA AGAGCAAGAA GAAAACTTGG AGTTTATCAT TGTGTCCCTC 
ACTGGCCAGA CGTGGCACTT TGAAGCCACC ACATACGAGG AGCGAGACGC ATGGGTCCAA 
GCCATCGAGA GCCAGATCCT AGCCAGCCTG CAGTCCTGTG AGAGCAGCAA GAATAAGTCC 
CGACTCACCA GCCAGAGCGA AGCCATGGCA CTGCAGTCAA TCCGTAACAT GAGAGGGAAC 
TCGCACTGCG TGGACTGTGA CACCCAGAAC CCCAACTGGG CCAGTTTGAA CTTGGGAGCC 
CTCATGTGTA TCGAGTGCTC AGGGATCCAC CGGAATCTCG GCACCCACCT CTCTCGGGTC 
CGATCGCTCG ACCTCGACGA CTGGCCCATG GAGCTGATCA AGGTGATGTC CTCCATCGGG 
AATGAGCTGG CCAACAGCGT GTGGGAAGAG GGCAGCCAGG GACGGACAAA GCCCTCGTTG 
GACTCCACCA GGGAAGAAAA GGAACGGTGG ATTCGGGCCA AGTATGAGCA GAAACTCTTC 
CTGGCCCCAC TGCCATGCAC AGAGTTCTCC CTGGGCCAGC AACTGCTACA TGCCACTGCT 
GAGGAAGACC TGCGGACTGT CATCCTGCTT CTGGCACACG GGTCACGGGA TGAGGTGAAT 
GAGACCTGTG GGGAAGGAGA TGGTCGCACG GCCCTGCACC TGGCCTGCCG CAAGGGCAAC 
GTGGTCCTGG TTCAGCTGCT GATCTGGTAC GGGGTGGACG TCATGGCCCG AGACGCCCAC 
GGGAACACAG CACTGGCCTA TGCCCGTCAG GCCTCCAGCC AGGAGTGTAT CGACGTGCTT 
CTACAGTATG GCTGCCCCGA CGAGCGCTTT GTGCTCATGG CTACCCCCAA CCTGTCTAGA 
AAGAACAACA GCAGGAACAA CAGTAGCGGC AGGGCGCCCA GTGTCATCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017451981.1
CDS	2465..6595
Translation	MTLQQAGPGERSIGLLREADPLRAVGIHPSAWACPPPRSPPPPRSRDPQAPLGARPAVSARRPPPARPAPARISILNRRPPAPRPTAAGATTRAARNLGRGGGSRTAELCRKRPWDALRPGALDNEAAGAAARRGLCAPRPHLLARRVPCLSAHPAPHGSALRVRTPAVGPRGTRTGGLQGRCSRSLSAGLAARGGRGRRSPPARALHLASSAPEARVPAAGGAGCSRASEGGEAAAAPGDMYLLDGSGEPASSPADTMSRGMPPRKTVYRISVTMVRKEQLAAPAPRPTRRPRPARSPEAPGRLEEEAEEAAAEDPEIGPRAWDFRAFRTRSTGQLELGRLRPCAHDTEPTDLMGSPTTEGPSSPDGEEARAAPLRRSLSFRHWSEPEVPRSPALSGGRRHSSSGSLAWMPDDDVEADVVPEPVAGVQPAAEKRNTLDVGEVLSKNDALSDLERWERSKSKNRTLDNSDLQRLERARAAAAGPGAASEHRLLRFFSGIFARRDGGPSPRSGALRSRGYFSLRRAPADTHSSSAESIEGSPRRDAFVNSQEWTLSRSVPELKVGIVGNLASGKSALVHRYLTGTYVQEESPEDMDAGGRFKKEIVVDGQSYLLLIRDEGGPPEAQFAMWVDAVIFVFSLEDEISFQTVYHYYSRMANYRNTSEIPLVLVGTQDAISSTNPRVIDDVRARKLSNDLKRCTYYETCATYGLNVERVFQDVAQKIVATRKKQQLSIGPCKSLPNSPSHSSVCSAQVSAVHISQTSNGGGSLSDYSSSVPSTPSTSQKELRIDVPPTANTPTPVRKQSKRRSNLFTSRKGSDPDKEKKGLESRADSIGSGRAIPIKQGMLLKRSGKSLNKEWKKKYVTLCDNGVLTYHPSLHDYMQNVHGKEIDLLRTTVKVPGKRPPRATSACAPISSPKTNGLAKDMSSLHISPNSGNVTSASGSQMASGISLVSFNSRPDGMHQRSYSVSSADQWSDATVIANSAISSDTGLGDSVCSSPSISSSTSPKLDPPPSPHANRKKHRRKKSTSNFKADGLTGTAEAKRKAWKLNRVGSLRNIYSSSSTNTEEQEENLEFIIVSLTGQTWHFEATTYEERDAWVQAIESQILASLQSCESSKNKSRLTSQSEAMALQSIRNMRGNSHCVDCDTQNPNWASLNLGALMCIECSGIHRNLGTHLSRVRSLDLDDWPMELIKVMSSIGNELANSVWEEGSQGRTKPSLDSTREEKERWIRAKYEQKLFLAPLPCTEFSLGQQLLHATAEEDLRTVILLLAHGSRDEVNETCGEGDGRTALHLACRKGNVVLVQLLIWYGVDVMARDAHGNTALAYARQASSQECIDVLLQYGCPDERFVLMATPNLSRKNNSRNNSSGRAPSVI

Target ORF information:

RefSeq Version	XM_017596492.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ArfGAP with GTPase domain, ankyrin repeat and PH domain 1 (Agap1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017596492.1

ORF Insert Sequence:

ATGACCTTGC AGCAAGCAGG TCCTGGAGAG CGCAGTATAG GATTACTGCG CGAAGCTGAT 
CCGCTCCGCG CGGTGGGGAT CCATCCCAGC GCTTGGGCTT GCCCTCCGCC GCGCTCGCCG 
CCGCCTCCGC GCTCCCGGGA CCCGCAGGCC CCGCTGGGCG CGCGGCCCGC AGTCTCCGCA 
CGTCGGCCTC CGCCCGCCCG CCCCGCCCCG GCGCGCATTT CCATTTTAAA CCGCCGCCCG 
CCCGCCCCTC GACCGACCGC GGCCGGCGCA ACCACCCGCG CGGCACGGAA CCTCGGGAGA 
GGCGGAGGCT CCCGGACTGC CGAGCTGTGC AGGAAAAGGC CCTGGGATGC GCTTCGGCCC 
GGCGCACTAG ACAATGAAGC CGCGGGCGCT GCTGCCCGCC GCGGGCTTTG CGCACCCAGG 
CCGCACCTGC TGGCGAGGCG GGTTCCGTGC CTGAGCGCCC ACCCAGCCCC TCATGGCTCG 
GCTCTCCGGG TGCGGACCCC GGCAGTCGGG CCGCGTGGCA CGCGGACGGG CGGACTCCAG 
GGGCGCTGTT CCCGCAGCCT GAGCGCGGGC CTCGCTGCGC GCGGGGGGCG TGGGCGCCGC 
TCCCCGCCCG CTCGGGCTCT GCACCTGGCC AGCTCTGCGC CTGAGGCGCG CGTCCCGGCA 
GCCGGCGGCG CTGGATGCAG CCGGGCCTCG GAGGGCGGCG AGGCGGCCGC GGCCCCTGGG 
GACATGTACC TGCTGGACGG CAGCGGCGAG CCCGCGTCTT CGCCTGCGGA CACGATGTCG 
CGCGGGATGC CCCCCAGGAA GACGGTCTAC CGCATCTCGG TGACCATGGT CAGGAAGGAG 
CAGCTGGCCG CTCCGGCTCC ACGTCCTACT CGGCGGCCCC GGCCCGCGCG CTCCCCGGAG 
GCGCCCGGGC GGCTGGAGGA GGAGGCCGAG GAGGCGGCGG CTGAGGATCC TGAGATCGGG 
CCGCGCGCCT GGGACTTCCG CGCCTTTCGC ACACGCAGCA CCGGGCAGCT GGAGCTCGGG 
CGGCTGCGGC CGTGCGCACA CGACACAGAA CCCACCGACC TGATGGGTAG CCCCACGACT 
GAGGGGCCCA GCAGTCCTGA TGGGGAGGAG GCACGGGCCG CGCCCCTGCG GCGGAGCCTC 
AGCTTTAGGC ACTGGAGCGA GCCCGAGGTG CCACGGAGCC CTGCGCTGAG CGGCGGGAGG 
CGCCACAGCA GCTCTGGAAG CCTGGCATGG ATGCCCGACG ACGATGTCGA GGCGGACGTC 
GTTCCGGAAC CGGTGGCTGG AGTGCAGCCG GCTGCTGAGA AGCGCAACAC CTTGGACGTG 
GGCGAGGTGC TTAGCAAGAA CGATGCCCTC TCAGACCTGG AGCGCTGGGA GCGCAGCAAG 
AGCAAGAACC GCACGCTGGA CAACAGCGAC CTGCAGCGGC TGGAGCGCGC GCGGGCCGCG 
GCTGCCGGCC CTGGGGCCGC CTCGGAGCAC CGGCTGCTGC GTTTCTTCAG TGGCATCTTC 
GCGCGCAGGG ACGGCGGGCC CTCACCCCGC AGCGGCGCCC TACGCTCGCG CGGTTACTTC 
AGCCTGCGGC GGGCCCCGGC CGACACGCAT TCGTCCAGTG CCGAGAGCAT CGAGGGGTCC 
CCGCGCAGAG ATGCTTTCGT GAACAGCCAG GAATGGACAC TCAGCCGATC AGTACCAGAG 
CTCAAAGTGG GAATTGTGGG TAACCTGGCC AGTGGCAAGT CTGCGCTGGT GCACCGGTAC 
CTGACAGGCA CATACGTGCA GGAGGAGTCT CCGGAAGATA TGGACGCAGG TGGCAGGTTC 
AAGAAGGAGA TTGTTGTTGA TGGACAGAGT TATCTGCTGC TGATTAGGGA TGAAGGGGGT 
CCCCCGGAGG CACAGTTTGC CATGTGGGTG GACGCGGTCA TCTTTGTCTT CAGCTTGGAG 
GATGAGATCA GTTTCCAGAC CGTCTACCAT TACTACAGCC GAATGGCCAA CTACAGGAAC 
ACCAGTGAGA TCCCATTAGT GCTGGTGGGG ACCCAGGACG CCATAAGTTC CACCAACCCG 
AGGGTCATCG ATGACGTCAG GGCTCGGAAG CTCTCCAATG ACCTGAAGCG GTGCACGTAC 
TATGAGACGT GCGCCACCTA TGGGCTCAAC GTGGAGCGGG TCTTCCAGGA CGTTGCCCAG 
AAGATTGTTG CCACGAGGAA GAAGCAGCAG CTGTCCATAG GCCCCTGCAA GTCCTTACCC 
AACTCTCCAA GCCACTCCTC TGTGTGTTCT GCCCAAGTGT CCGCAGTACA CATCAGCCAG 
ACAAGTAACG GAGGTGGGAG TTTAAGCGAC TACTCATCCT CTGTCCCTTC GACCCCAAGC 
ACCAGCCAGA AGGAGCTTCG GATCGACGTC CCTCCCACTG CCAACACTCC AACGCCCGTC 
CGGAAGCAAT CCAAGCGCAG GTCCAACCTA TTCACATCTA GGAAAGGGAG CGACCCAGAC 
AAAGAGAAGA AAGGCCTGGA GAGCCGCGCG GACAGCATCG GGAGCGGGCG AGCCATCCCA 
ATTAAACAGG GCATGCTCCT GAAAAGAAGT GGCAAGTCAC TAAACAAAGA GTGGAAAAAG 
AAGTACGTCA CCCTATGCGA CAATGGTGTG CTGACCTACC ACCCCAGTTT ACATGACTAC 
ATGCAGAACG TCCATGGCAA AGAGATAGAC CTCTTGAGAA CCACTGTGAA GGTGCCGGGG 
AAGAGGCCGC CCCGAGCCAC ATCAGCCTGT GCGCCGATCT CCAGTCCCAA AACCAATGGC 
CTGGCCAAGG ACATGAGCAG TTTACACATC TCACCAAATT CAGGGAATGT CACTAGTGCG 
TCTGGGTCTC AGATGGCAAG CGGCATCAGC CTGGTCTCCT TCAACAGCCG ACCCGATGGC 
ATGCACCAGC GCTCCTACTC AGTCTCCAGT GCCGACCAGT GGAGTGACGC TACAGTCATT 
GCAAACTCAG CCATCAGCAG TGACACGGGG CTGGGTGACT CGGTGTGTTC CAGCCCAAGT 
ATCTCCAGCA GCACCAGCCC CAAGCTCGAC CCACCCCCCT CCCCCCACGC CAACAGAAAG 
AAGCATCGGA GGAAGAAAAG TACCAGCAAC TTCAAGGCCG ACGGGCTCAC GGGCACCGCT 
GAAGCCAAAC GCAAAGCATG GAAACTAAAC CGTGTTGGTA GCCTGCGAAA CATATACAGC 
AGCAGCAGCA CCAACACCGA AGAGCAAGAA GAAAACTTGG AGTTTATCAT TGTGTCCCTC 
ACTGGCCAGA CGTGGCACTT TGAAGCCACC ACATACGAGG AGCGAGACGC ATGGGTCCAA 
GCCATCGAGA GCCAGATCCT AGCCAGCCTG CAGTCCTGTG AGAGCAGCAA GAATAAGTCC 
CGACTCACCA GCCAGAGCGA AGCCATGGCA CTGCAGTCAA TCCGTAACAT GAGAGGGAAC 
TCGCACTGCG TGGACTGTGA CACCCAGAAC CCCAACTGGG CCAGTTTGAA CTTGGGAGCC 
CTCATGTGTA TCGAGTGCTC AGGGATCCAC CGGAATCTCG GCACCCACCT CTCTCGGGTC 
CGATCGCTCG ACCTCGACGA CTGGCCCATG GAGCTGATCA AGGTGATGTC CTCCATCGGG 
AATGAGCTGG CCAACAGCGT GTGGGAAGAG GGCAGCCAGG GACGGACAAA GCCCTCGTTG 
GACTCCACCA GGGAAGAAAA GGAACGGTGG ATTCGGGCCA AGTATGAGCA GAAACTCTTC 
CTGGCCCCAC TGCCATGCAC AGAGTTCTCC CTGGGCCAGC AACTGCTACA TGCCACTGCT 
GAGGAAGACC TGCGGACTGT CATCCTGCTT CTGGCACACG GGTCACGGGA TGAGGTGAAT 
GAGACCTGTG GGGAAGGAGA TGGTCGCACG GCCCTGCACC TGGCCTGCCG CAAGGGCAAC 
GTGGTCCTGG TTCAGCTGCT GATCTGGTAC GGGGTGGACG TCATGGCCCG AGACGCCCAC 
GGGAACACAG CACTGGCCTA TGCCCGTCAG GCCTCCAGCC AGGAGTGTAT CGACGTGCTT 
CTACAGTATG GCTGCCCCGA CGAGCGCTTT GTGCTCATGG CTACCCCCAA CCTGTCTAGA 
AAGAACAACA GCAGGAACAA CAGTAGCGGC AGGGCGCCCA GTGTCATCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Agap1 ( XM_017596493.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017451982.1 Rattus norvegicus ArfGAP with GTPase domain, ankyrin repeat and PH domain 1 (Agap1), transcript variant X5, mRNA.

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CloneID	ORa62786
Clone ID Related Accession (Same CDS sequence)	XM_017596493.1
Accession Version	XM_017596493.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4107bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	arf-GAP with GTPase, ANK repeat and PH domain-containing protein 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005108.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCTTGC AGCAAGCAGG TCCTGGAGAG CGCAGTATAG GATTACTGCG CGAAGCTGAT 
CCGCTCCGCG CGGTGGGGAT CCATCCCAGC GCTTGGGCTT GCCCTCCGCC GCGCTCGCCG 
CCGCCTCCGC GCTCCCGGGA CCCGCAGGCC CCGCTGGGCG CGCGGCCCGC AGTCTCCGCA 
CGTCGGCCTC CGCCCGCCCG CCCCGCCCCG GCGCGCATTT CCATTTTAAA CCGCCGCCCG 
CCCGCCCCTC GACCGACCGC GGCCGGCGCA ACCACCCGCG CGGCACGGAA CCTCGGGAGA 
GGCGGAGGCT CCCGGACTGC CGAGCTGTGC AGGAAAAGGC CCTGGGATGC GCTTCGGCCC 
GGCGCACTAG ACAATGAAGC CGCGGGCGCT GCTGCCCGCC GCGGGCTTTG CGCACCCAGG 
CCGCACCTGC TGGCGAGGCG GGTTCCGTGC CTGAGCGCCC ACCCAGCCCC TCATGGCTCG 
GCTCTCCGGG TGCGGACCCC GGCAGTCGGG CCGCGTGGCA CGCGGACGGG CGGACTCCAG 
GGGCGCTGTT CCCGCAGCCT GAGCGCGGGC CTCGCTGCGC GCGGGGGGCG TGGGCGCCGC 
TCCCCGCCCG CTCGGGCTCT GCACCTGGCC AGCTCTGCGC CTGAGGCGCG CGTCCCGGCA 
GCCGGCGGCG CTGGATGCAG CCGGGCCTCG GAGGGCGGCG AGGCGGCCGC GGCCCCTGGG 
GACATGTACC TGCTGGACGG CAGCGGCGAG CCCGCGTCTT CGCCTGCGGA CACGATGTCG 
CGCGGGATGC CCCCCAGGAA GACGGTCTAC CGCATCTCGG TGACCATGGT CAGGAAGGAG 
CAGCTGGCCG CTCCGGCTCC ACGTCCTACT CGGCGGCCCC GGCCCGCGCG CTCCCCGGAG 
GCGCCCGGGC GGCTGGAGGA GGAGGCCGAG GAGGCGGCGG CTGAGGATCC TGAGATCGGG 
CCGCGCGCCT GGGACTTCCG CGCCTTTCGC ACACGCAGCA CCGGGCAGCT GGAGCTCGGG 
CGGCTGCGGC CGTGCGCACA CGACACAGAA CCCACCGACC TGATGGGTAG CCCCACGACT 
GAGGGGCCCA GCAGTCCTGA TGGGGAGGAG GCACGGGCCG CGCCCCTGCG GCGGAGCCTC 
AGCTTTAGGC ACTGGAGCGA GCCCGAGGTG CCACGGAGCC CTGCGCTGAG CGGCGGGAGG 
CGCCACAGCA GCTCTGGAAG CCTGGCATGG ATGCCCGACG ACGATGTCGA GGCGGACGTC 
GTTCCGGAAC CGGTGGCTGG AGTGCAGCCG GCTGCTGAGA AGCGCAACAC CTTGGACGTG 
GGCGAGGTGC TTAGCAAGAA CGATGCCCTC TCAGACCTGG AGCGCTGGGA GCGCAGCAAG 
AGCAAGAACC GCACGCTGGA CAACAGCGAC CTGCAGCGGC TGGAGCGCGC GCGGGCCGCG 
GCTGCCGGCC CTGGGGCCGC CTCGGAGCAC CGGCTGCTGC GTTTCTTCAG TGGCATCTTC 
GCGCGCAGGG ACGGCGGGCC CTCACCCCGC AGCGGCGCCC TACGCTCGCG CGGTTACTTC 
AGCCTGCGGC GGGCCCCGGC CGACACGCAT TCGTCCAGTG CCGAGAGCAT CGAGGGGTCC 
CCGCGCAGAG ATGCTTTCGT GAACAGCCAG GAATGGACAC TCAGCCGATC AGTACCAGAG 
CTCAAAGTGG GAATTGTGGG TAACCTGGCC AGTGGCAAGT CTGCGCTGGT GCACCGGTAC 
CTGACAGGCA CATACGTGCA GGAGGAGTCT CCGGAAGATA TGGACGCAGG TGGCAGGTTC 
AAGAAGGAGA TTGTTGTTGA TGGACAGAGT TATCTGCTGC TGATTAGGGA TGAAGGGGGT 
CCCCCGGAGG CACAGTTTGC CATGTGGGTG GACGCGGTCA TCTTTGTCTT CAGCTTGGAG 
GATGAGATCA GTTTCCAGAC CGTCTACCAT TACTACAGCC GAATGGCCAA CTACAGGAAC 
ACCAGTGAGA TCCCATTAGT GCTGGTGGGG ACCCAGGACG CCATAAGTTC CACCAACCCG 
AGGGTCATCG ATGACGTCAG GGCTCGGAAG CTCTCCAATG ACCTGAAGCG GTGCACGTAC 
TATGAGACGT GCGCCACCTA TGGGCTCAAC GTGGAGCGGG TCTTCCAGGA CGTTGCCCAG 
AAGATTGTTG CCACGAGGAA GAAGCAGCAG CTGTCCATAG GCCCCTGCAA GTCCTTACCC 
AACTCTCCAA GCCACTCCTC TGTGTGTTCT GCCCAAGTGT CCGCAGTACA CATCAGCCAG 
ACAAGTAACG GAGGTGGGAG TTTAAGCGAC TACTCATCCT CTGTCCCTTC GACCCCAAGC 
ACCAGCCAGA AGGAGCTTCG GATCGACGTC CCTCCCACTG CCAACACTCC AACGCCCGTC 
CGGAAGCAAT CCAAGCGCAG GTCCAACCTA TTCACATCTA GGAAAGGGAG CGACCCAGAC 
AAAGAGAAGA AAGGCCTGGA GAGCCGCGCG GACAGCATCG GGAGCGGGCG AGCCATCCCA 
ATTAAACAGG GCATGCTCCT GAAAAGAAGT GGCAAGTCAC TAAACAAAGA GTGGAAAAAG 
AAGTACGTCA CCCTATGCGA CAATGGTGTG CTGACCTACC ACCCCAGTTT ACATGACTAC 
ATGCAGAACG TCCATGGCAA AGAGATAGAC CTCTTGAGAA CCACTGTGAA GGTGCCGGGG 
AAGAGGCCGC CCCGAGCCAC ATCAGCCTGT GCGCCGATCT CCAGTCCCAA AACCAATGGC 
CTGGCCAAGG ACATGAGCAG TTTACACATC TCACCAAATT CAGACACGGG GCTGGGTGAC 
TCGGTGTGTT CCAGCCCAAG TATCTCCAGC AGCACCAGCC CCAAGCTCGA CCCACCCCCC 
TCCCCCCACG CCAACAGAAA GAAGCATCGG AGGAAGAAAA GTACCAGCAA CTTCAAGGCC 
GACGGGCTCA CGGGCACCGC TGAAGGTTCA CATTCACACT CTGGCCTCAG ACTGAGAGAC 
CCAGTTGCCT GGCGCCCTGG AGATCTCCAT CCACAGTTCC ATACATCTTT AGGCCAGAAA 
AGAGCCCCAC GGCCCCATCT TGGGAGCTTC TGGGTTTCTG CCAAACGCAA AGCATGGAAA 
CTAAACCGTG TTGGTAGCCT GCGAAACATA TACAGCAGCA GCAGCACCAA CACCGAAGAG 
CAAGAAGAAA ACTTGGAGTT TATCATTGTG TCCCTCACTG GCCAGACGTG GCACTTTGAA 
GCCACCACAT ACGAGGAGCG AGACGCATGG GTCCAAGCCA TCGAGAGCCA GATCCTAGCC 
AGCCTGCAGT CCTGTGAGAG CAGCAAGAAT AAGTCCCGAC TCACCAGCCA GAGCGAAGCC 
ATGGCACTGC AGTCAATCCG TAACATGAGA GGGAACTCGC ACTGCGTGGA CTGTGACACC 
CAGAACCCCA ACTGGGCCAG TTTGAACTTG GGAGCCCTCA TGTGTATCGA GTGCTCAGGG 
ATCCACCGGA ATCTCGGCAC CCACCTCTCT CGGGTCCGAT CGCTCGACCT CGACGACTGG 
CCCATGGAGC TGATCAAGGT GATGTCCTCC ATCGGGAATG AGCTGGCCAA CAGCGTGTGG 
GAAGAGGGCA GCCAGGGACG GACAAAGCCC TCGTTGGACT CCACCAGGGA AGAAAAGGAA 
CGGTGGATTC GGGCCAAGTA TGAGCAGAAA CTCTTCCTGG CCCCACTGCC ATGCACAGAG 
TTCTCCCTGG GCCAGCAACT GCTACATGCC ACTGCTGAGG AAGACCTGCG GACTGTCATC 
CTGCTTCTGG CACACGGGTC ACGGGATGAG GTGAATGAGA CCTGTGGGGA AGGAGATGGT 
CGCACGGCCC TGCACCTGGC CTGCCGCAAG GGCAACGTGG TCCTGGTTCA GCTGCTGATC 
TGGTACGGGG TGGACGTCAT GGCCCGAGAC GCCCACGGGA ACACAGCACT GGCCTATGCC 
CGTCAGGCCT CCAGCCAGGA GTGTATCGAC GTGCTTCTAC AGTATGGCTG CCCCGACGAG 
CGCTTTGTGC TCATGGCTAC CCCCAACCTG TCTAGAAAGA ACAACAGCAG GAACAACAGT 
AGCGGCAGGG CGCCCAGTGT CATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017451982.1
CDS	2466..6572
Translation	MTLQQAGPGERSIGLLREADPLRAVGIHPSAWACPPPRSPPPPRSRDPQAPLGARPAVSARRPPPARPAPARISILNRRPPAPRPTAAGATTRAARNLGRGGGSRTAELCRKRPWDALRPGALDNEAAGAAARRGLCAPRPHLLARRVPCLSAHPAPHGSALRVRTPAVGPRGTRTGGLQGRCSRSLSAGLAARGGRGRRSPPARALHLASSAPEARVPAAGGAGCSRASEGGEAAAAPGDMYLLDGSGEPASSPADTMSRGMPPRKTVYRISVTMVRKEQLAAPAPRPTRRPRPARSPEAPGRLEEEAEEAAAEDPEIGPRAWDFRAFRTRSTGQLELGRLRPCAHDTEPTDLMGSPTTEGPSSPDGEEARAAPLRRSLSFRHWSEPEVPRSPALSGGRRHSSSGSLAWMPDDDVEADVVPEPVAGVQPAAEKRNTLDVGEVLSKNDALSDLERWERSKSKNRTLDNSDLQRLERARAAAAGPGAASEHRLLRFFSGIFARRDGGPSPRSGALRSRGYFSLRRAPADTHSSSAESIEGSPRRDAFVNSQEWTLSRSVPELKVGIVGNLASGKSALVHRYLTGTYVQEESPEDMDAGGRFKKEIVVDGQSYLLLIRDEGGPPEAQFAMWVDAVIFVFSLEDEISFQTVYHYYSRMANYRNTSEIPLVLVGTQDAISSTNPRVIDDVRARKLSNDLKRCTYYETCATYGLNVERVFQDVAQKIVATRKKQQLSIGPCKSLPNSPSHSSVCSAQVSAVHISQTSNGGGSLSDYSSSVPSTPSTSQKELRIDVPPTANTPTPVRKQSKRRSNLFTSRKGSDPDKEKKGLESRADSIGSGRAIPIKQGMLLKRSGKSLNKEWKKKYVTLCDNGVLTYHPSLHDYMQNVHGKEIDLLRTTVKVPGKRPPRATSACAPISSPKTNGLAKDMSSLHISPNSDTGLGDSVCSSPSISSSTSPKLDPPPSPHANRKKHRRKKSTSNFKADGLTGTAEGSHSHSGLRLRDPVAWRPGDLHPQFHTSLGQKRAPRPHLGSFWVSAKRKAWKLNRVGSLRNIYSSSSTNTEEQEENLEFIIVSLTGQTWHFEATTYEERDAWVQAIESQILASLQSCESSKNKSRLTSQSEAMALQSIRNMRGNSHCVDCDTQNPNWASLNLGALMCIECSGIHRNLGTHLSRVRSLDLDDWPMELIKVMSSIGNELANSVWEEGSQGRTKPSLDSTREEKERWIRAKYEQKLFLAPLPCTEFSLGQQLLHATAEEDLRTVILLLAHGSRDEVNETCGEGDGRTALHLACRKGNVVLVQLLIWYGVDVMARDAHGNTALAYARQASSQECIDVLLQYGCPDERFVLMATPNLSRKNNSRNNSSGRAPSVI

Target ORF information:

RefSeq Version	XM_017596493.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ArfGAP with GTPase domain, ankyrin repeat and PH domain 1 (Agap1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017596493.1

ORF Insert Sequence:

ATGACCTTGC AGCAAGCAGG TCCTGGAGAG CGCAGTATAG GATTACTGCG CGAAGCTGAT 
CCGCTCCGCG CGGTGGGGAT CCATCCCAGC GCTTGGGCTT GCCCTCCGCC GCGCTCGCCG 
CCGCCTCCGC GCTCCCGGGA CCCGCAGGCC CCGCTGGGCG CGCGGCCCGC AGTCTCCGCA 
CGTCGGCCTC CGCCCGCCCG CCCCGCCCCG GCGCGCATTT CCATTTTAAA CCGCCGCCCG 
CCCGCCCCTC GACCGACCGC GGCCGGCGCA ACCACCCGCG CGGCACGGAA CCTCGGGAGA 
GGCGGAGGCT CCCGGACTGC CGAGCTGTGC AGGAAAAGGC CCTGGGATGC GCTTCGGCCC 
GGCGCACTAG ACAATGAAGC CGCGGGCGCT GCTGCCCGCC GCGGGCTTTG CGCACCCAGG 
CCGCACCTGC TGGCGAGGCG GGTTCCGTGC CTGAGCGCCC ACCCAGCCCC TCATGGCTCG 
GCTCTCCGGG TGCGGACCCC GGCAGTCGGG CCGCGTGGCA CGCGGACGGG CGGACTCCAG 
GGGCGCTGTT CCCGCAGCCT GAGCGCGGGC CTCGCTGCGC GCGGGGGGCG TGGGCGCCGC 
TCCCCGCCCG CTCGGGCTCT GCACCTGGCC AGCTCTGCGC CTGAGGCGCG CGTCCCGGCA 
GCCGGCGGCG CTGGATGCAG CCGGGCCTCG GAGGGCGGCG AGGCGGCCGC GGCCCCTGGG 
GACATGTACC TGCTGGACGG CAGCGGCGAG CCCGCGTCTT CGCCTGCGGA CACGATGTCG 
CGCGGGATGC CCCCCAGGAA GACGGTCTAC CGCATCTCGG TGACCATGGT CAGGAAGGAG 
CAGCTGGCCG CTCCGGCTCC ACGTCCTACT CGGCGGCCCC GGCCCGCGCG CTCCCCGGAG 
GCGCCCGGGC GGCTGGAGGA GGAGGCCGAG GAGGCGGCGG CTGAGGATCC TGAGATCGGG 
CCGCGCGCCT GGGACTTCCG CGCCTTTCGC ACACGCAGCA CCGGGCAGCT GGAGCTCGGG 
CGGCTGCGGC CGTGCGCACA CGACACAGAA CCCACCGACC TGATGGGTAG CCCCACGACT 
GAGGGGCCCA GCAGTCCTGA TGGGGAGGAG GCACGGGCCG CGCCCCTGCG GCGGAGCCTC 
AGCTTTAGGC ACTGGAGCGA GCCCGAGGTG CCACGGAGCC CTGCGCTGAG CGGCGGGAGG 
CGCCACAGCA GCTCTGGAAG CCTGGCATGG ATGCCCGACG ACGATGTCGA GGCGGACGTC 
GTTCCGGAAC CGGTGGCTGG AGTGCAGCCG GCTGCTGAGA AGCGCAACAC CTTGGACGTG 
GGCGAGGTGC TTAGCAAGAA CGATGCCCTC TCAGACCTGG AGCGCTGGGA GCGCAGCAAG 
AGCAAGAACC GCACGCTGGA CAACAGCGAC CTGCAGCGGC TGGAGCGCGC GCGGGCCGCG 
GCTGCCGGCC CTGGGGCCGC CTCGGAGCAC CGGCTGCTGC GTTTCTTCAG TGGCATCTTC 
GCGCGCAGGG ACGGCGGGCC CTCACCCCGC AGCGGCGCCC TACGCTCGCG CGGTTACTTC 
AGCCTGCGGC GGGCCCCGGC CGACACGCAT TCGTCCAGTG CCGAGAGCAT CGAGGGGTCC 
CCGCGCAGAG ATGCTTTCGT GAACAGCCAG GAATGGACAC TCAGCCGATC AGTACCAGAG 
CTCAAAGTGG GAATTGTGGG TAACCTGGCC AGTGGCAAGT CTGCGCTGGT GCACCGGTAC 
CTGACAGGCA CATACGTGCA GGAGGAGTCT CCGGAAGATA TGGACGCAGG TGGCAGGTTC 
AAGAAGGAGA TTGTTGTTGA TGGACAGAGT TATCTGCTGC TGATTAGGGA TGAAGGGGGT 
CCCCCGGAGG CACAGTTTGC CATGTGGGTG GACGCGGTCA TCTTTGTCTT CAGCTTGGAG 
GATGAGATCA GTTTCCAGAC CGTCTACCAT TACTACAGCC GAATGGCCAA CTACAGGAAC 
ACCAGTGAGA TCCCATTAGT GCTGGTGGGG ACCCAGGACG CCATAAGTTC CACCAACCCG 
AGGGTCATCG ATGACGTCAG GGCTCGGAAG CTCTCCAATG ACCTGAAGCG GTGCACGTAC 
TATGAGACGT GCGCCACCTA TGGGCTCAAC GTGGAGCGGG TCTTCCAGGA CGTTGCCCAG 
AAGATTGTTG CCACGAGGAA GAAGCAGCAG CTGTCCATAG GCCCCTGCAA GTCCTTACCC 
AACTCTCCAA GCCACTCCTC TGTGTGTTCT GCCCAAGTGT CCGCAGTACA CATCAGCCAG 
ACAAGTAACG GAGGTGGGAG TTTAAGCGAC TACTCATCCT CTGTCCCTTC GACCCCAAGC 
ACCAGCCAGA AGGAGCTTCG GATCGACGTC CCTCCCACTG CCAACACTCC AACGCCCGTC 
CGGAAGCAAT CCAAGCGCAG GTCCAACCTA TTCACATCTA GGAAAGGGAG CGACCCAGAC 
AAAGAGAAGA AAGGCCTGGA GAGCCGCGCG GACAGCATCG GGAGCGGGCG AGCCATCCCA 
ATTAAACAGG GCATGCTCCT GAAAAGAAGT GGCAAGTCAC TAAACAAAGA GTGGAAAAAG 
AAGTACGTCA CCCTATGCGA CAATGGTGTG CTGACCTACC ACCCCAGTTT ACATGACTAC 
ATGCAGAACG TCCATGGCAA AGAGATAGAC CTCTTGAGAA CCACTGTGAA GGTGCCGGGG 
AAGAGGCCGC CCCGAGCCAC ATCAGCCTGT GCGCCGATCT CCAGTCCCAA AACCAATGGC 
CTGGCCAAGG ACATGAGCAG TTTACACATC TCACCAAATT CAGACACGGG GCTGGGTGAC 
TCGGTGTGTT CCAGCCCAAG TATCTCCAGC AGCACCAGCC CCAAGCTCGA CCCACCCCCC 
TCCCCCCACG CCAACAGAAA GAAGCATCGG AGGAAGAAAA GTACCAGCAA CTTCAAGGCC 
GACGGGCTCA CGGGCACCGC TGAAGGTTCA CATTCACACT CTGGCCTCAG ACTGAGAGAC 
CCAGTTGCCT GGCGCCCTGG AGATCTCCAT CCACAGTTCC ATACATCTTT AGGCCAGAAA 
AGAGCCCCAC GGCCCCATCT TGGGAGCTTC TGGGTTTCTG CCAAACGCAA AGCATGGAAA 
CTAAACCGTG TTGGTAGCCT GCGAAACATA TACAGCAGCA GCAGCACCAA CACCGAAGAG 
CAAGAAGAAA ACTTGGAGTT TATCATTGTG TCCCTCACTG GCCAGACGTG GCACTTTGAA 
GCCACCACAT ACGAGGAGCG AGACGCATGG GTCCAAGCCA TCGAGAGCCA GATCCTAGCC 
AGCCTGCAGT CCTGTGAGAG CAGCAAGAAT AAGTCCCGAC TCACCAGCCA GAGCGAAGCC 
ATGGCACTGC AGTCAATCCG TAACATGAGA GGGAACTCGC ACTGCGTGGA CTGTGACACC 
CAGAACCCCA ACTGGGCCAG TTTGAACTTG GGAGCCCTCA TGTGTATCGA GTGCTCAGGG 
ATCCACCGGA ATCTCGGCAC CCACCTCTCT CGGGTCCGAT CGCTCGACCT CGACGACTGG 
CCCATGGAGC TGATCAAGGT GATGTCCTCC ATCGGGAATG AGCTGGCCAA CAGCGTGTGG 
GAAGAGGGCA GCCAGGGACG GACAAAGCCC TCGTTGGACT CCACCAGGGA AGAAAAGGAA 
CGGTGGATTC GGGCCAAGTA TGAGCAGAAA CTCTTCCTGG CCCCACTGCC ATGCACAGAG 
TTCTCCCTGG GCCAGCAACT GCTACATGCC ACTGCTGAGG AAGACCTGCG GACTGTCATC 
CTGCTTCTGG CACACGGGTC ACGGGATGAG GTGAATGAGA CCTGTGGGGA AGGAGATGGT 
CGCACGGCCC TGCACCTGGC CTGCCGCAAG GGCAACGTGG TCCTGGTTCA GCTGCTGATC 
TGGTACGGGG TGGACGTCAT GGCCCGAGAC GCCCACGGGA ACACAGCACT GGCCTATGCC 
CGTCAGGCCT CCAGCCAGGA GTGTATCGAC GTGCTTCTAC AGTATGGCTG CCCCGACGAG 
CGCTTTGTGC TCATGGCTAC CCCCAACCTG TCTAGAAAGA ACAACAGCAG GAACAACAGT 
AGCGGCAGGG CGCCCAGTGT CATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Agap1 ( XM_017596494.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017451983.1 Rattus norvegicus ArfGAP with GTPase domain, ankyrin repeat and PH domain 1 (Agap1), transcript variant X6, mRNA.

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CloneID	ORa62787
Clone ID Related Accession (Same CDS sequence)	XM_017596494.1
Accession Version	XM_017596494.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4053bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	arf-GAP with GTPase, ANK repeat and PH domain-containing protein 1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005108.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCTTGC AGCAAGCAGG TCCTGGAGAG CGCAGTATAG GATTACTGCG CGAAGCTGAT 
CCGCTCCGCG CGGTGGGGAT CCATCCCAGC GCTTGGGCTT GCCCTCCGCC GCGCTCGCCG 
CCGCCTCCGC GCTCCCGGGA CCCGCAGGCC CCGCTGGGCG CGCGGCCCGC AGTCTCCGCA 
CGTCGGCCTC CGCCCGCCCG CCCCGCCCCG GCGCGCATTT CCATTTTAAA CCGCCGCCCG 
CCCGCCCCTC GACCGACCGC GGCCGGCGCA ACCACCCGCG CGGCACGGAA CCTCGGGAGA 
GGCGGAGGCT CCCGGACTGC CGAGCTGTGC AGGAAAAGGC CCTGGGATGC GCTTCGGCCC 
GGCGCACTAG ACAATGAAGC CGCGGGCGCT GCTGCCCGCC GCGGGCTTTG CGCACCCAGG 
CCGCACCTGC TGGCGAGGCG GGTTCCGTGC CTGAGCGCCC ACCCAGCCCC TCATGGCTCG 
GCTCTCCGGG TGCGGACCCC GGCAGTCGGG CCGCGTGGCA CGCGGACGGG CGGACTCCAG 
GGGCGCTGTT CCCGCAGCCT GAGCGCGGGC CTCGCTGCGC GCGGGGGGCG TGGGCGCCGC 
TCCCCGCCCG CTCGGGCTCT GCACCTGGCC AGCTCTGCGC CTGAGGCGCG CGTCCCGGCA 
GCCGGCGGCG CTGGATGCAG CCGGGCCTCG GAGGGCGGCG AGGCGGCCGC GGCCCCTGGG 
GACATGTACC TGCTGGACGG CAGCGGCGAG CCCGCGTCTT CGCCTGCGGA CACGATGTCG 
CGCGGGATGC CCCCCAGGAA GACGGTCTAC CGCATCTCGG TGACCATGGT CAGGAAGGAG 
CAGCTGGCCG CTCCGGCTCC ACGTCCTACT CGGCGGCCCC GGCCCGCGCG CTCCCCGGAG 
GCGCCCGGGC GGCTGGAGGA GGAGGCCGAG GAGGCGGCGG CTGAGGATCC TGAGATCGGG 
CCGCGCGCCT GGGACTTCCG CGCCTTTCGC ACACGCAGCA CCGGGCAGCT GGAGCTCGGG 
CGGCTGCGGC CGTGCGCACA CGACACAGAA CCCACCGACC TGATGGGTAG CCCCACGACT 
GAGGGGCCCA GCAGTCCTGA TGGGGAGGAG GCACGGGCCG CGCCCCTGCG GCGGAGCCTC 
AGCTTTAGGC ACTGGAGCGA GCCCGAGGTG CCACGGAGCC CTGCGCTGAG CGGCGGGAGG 
CGCCACAGCA GCTCTGGAAG CCTGGCATGG ATGCCCGACG ACGATGTCGA GGCGGACGTC 
GTTCCGGAAC CGGTGGCTGG AGTGCAGCCG GCTGCTGAGA AGCGCAACAC CTTGGACGTG 
GGCGAGGTGC TTAGCAAGAA CGATGCCCTC TCAGACCTGG AGCGCTGGGA GCGCAGCAAG 
AGCAAGAACC GCACGCTGGA CAACAGCGAC CTGCAGCGGC TGGAGCGCGC GCGGGCCGCG 
GCTGCCGGCC CTGGGGCCGC CTCGGAGCAC CGGCTGCTGC GTTTCTTCAG TGGCATCTTC 
GCGCGCAGGG ACGGCGGGCC CTCACCCCGC AGCGGCGCCC TACGCTCGCG CGGTTACTTC 
AGCCTGCGGC GGGCCCCGGC CGACACGCAT TCGTCCAGTG CCGAGAGCAT CGAGGGGTCC 
CCGCGCAGAG ATGCTTTCGT GAACAGCCAG GAATGGACAC TCAGCCGATC AGTACCAGAG 
CTCAAAGTGG GAATTGTGGG TAACCTGGCC AGTGGCAAGT CTGCGCTGGT GCACCGGTAC 
CTGACAGGCA CATACGTGCA GGAGGAGTCT CCGGAAGATA TGGACGCAGG TGGCAGGTTC 
AAGAAGGAGA TTGTTGTTGA TGGACAGAGT TATCTGCTGC TGATTAGGGA TGAAGGGGGT 
CCCCCGGAGG CACAGTTTGC CATGTGGGTG GACGCGGTCA TCTTTGTCTT CAGCTTGGAG 
GATGAGATCA GTTTCCAGAC CGTCTACCAT TACTACAGCC GAATGGCCAA CTACAGGAAC 
ACCAGTGAGA TCCCATTAGT GCTGGTGGGG ACCCAGGACG CCATAAGTTC CACCAACCCG 
AGGGTCATCG ATGACGTCAG GGCTCGGAAG CTCTCCAATG ACCTGAAGCG GTGCACGTAC 
TATGAGACGT GCGCCACCTA TGGGCTCAAC GTGGAGCGGG TCTTCCAGGA CGTTGCCCAG 
AAGATTGTTG CCACGAGGAA GAAGCAGCAG CTGTCCATAG GCCCCTGCAA GTCCTTACCC 
AACTCTCCAA GCCACTCCTC TGTGTGTTCT GCCCAAGTGT CCGCAGTACA CATCAGCCAG 
ACAAGTAACG GAGGTGGGAG TTTAAGCGAC TACTCATCCT CTGTCCCTTC GACCCCAAGC 
ACCAGCCAGA AGGAGCTTCG GATCGACGTC CCTCCCACTG CCAACACTCC AACGCCCGTC 
CGGAAGCAAT CCAAGCGCAG GTCCAACCTA TTCACATCTA GGAAAGGGAG CGACCCAGAC 
AAAGAGAAGA AAGGCCTGGA GAGCCGCGCG GACAGCATCG GGAGCGGGCG AGCCATCCCA 
ATTAAACAGG GCATGCTCCT GAAAAGAAGT GGCAAGTCAC TAAACAAAGA GTGGAAAAAG 
AAGTACGTCA CCCTATGCGA CAATGGTGTG CTGACCTACC ACCCCAGTTT ACATGACTAC 
ATGCAGAACG TCCATGGCAA AGAGATAGAC CTCTTGAGAA CCACTGTGAA GGTGCCGGGG 
AAGAGGCCGC CCCGAGCCAC ATCAGCCTGT GCGCCGATCT CCAGTCCCAA AACCAATGGC 
CTGGCCAAGG ACATGAGCAG TTTACACATC TCACCAAATT CAGGGAATGT CACTAGTGCG 
TCTGGGTCTC AGATGGCAAG CGGCATCAGC CTGGTCTCCT TCAACAGCCG ACCCGATGGC 
ATGCACCAGC GCTCCTACTC AGTCTCCAGT GCCGACCAGT GGAGTGACGC TACAGTCATT 
GCAAACTCAG CCATCAGCAG TGACACGGGG CTGGGTGACT CGGTGTGTTC CAGCCCAAGT 
ATCTCCAGCA GCACCAGCCC CAAGCTCGAC CCACCCCCCT CCCCCCACGC CAACAGAAAG 
AAGCATCGGA GGAAGAAAAG TACCAGCAAC TTCAAGGCCG ACGGGCTCAC GGGCACCGCT 
GAAGAGCAAG AAGAAAACTT GGAGTTTATC ATTGTGTCCC TCACTGGCCA GACGTGGCAC 
TTTGAAGCCA CCACATACGA GGAGCGAGAC GCATGGGTCC AAGCCATCGA GAGCCAGATC 
CTAGCCAGCC TGCAGTCCTG TGAGAGCAGC AAGAATAAGT CCCGACTCAC CAGCCAGAGC 
GAAGCCATGG CACTGCAGTC AATCCGTAAC ATGAGAGGGA ACTCGCACTG CGTGGACTGT 
GACACCCAGA ACCCCAACTG GGCCAGTTTG AACTTGGGAG CCCTCATGTG TATCGAGTGC 
TCAGGGATCC ACCGGAATCT CGGCACCCAC CTCTCTCGGG TCCGATCGCT CGACCTCGAC 
GACTGGCCCA TGGAGCTGAT CAAGGTGATG TCCTCCATCG GGAATGAGCT GGCCAACAGC 
GTGTGGGAAG AGGGCAGCCA GGGACGGACA AAGCCCTCGT TGGACTCCAC CAGGGAAGAA 
AAGGAACGGT GGATTCGGGC CAAGTATGAG CAGAAACTCT TCCTGGCCCC ACTGCCATGC 
ACAGAGTTCT CCCTGGGCCA GCAACTGCTA CATGCCACTG CTGAGGAAGA CCTGCGGACT 
GTCATCCTGC TTCTGGCACA CGGGTCACGG GATGAGGTGA ATGAGACCTG TGGGGAAGGA 
GATGGTCGCA CGGCCCTGCA CCTGGCCTGC CGCAAGGGCA ACGTGGTCCT GGTTCAGCTG 
CTGATCTGGT ACGGGGTGGA CGTCATGGCC CGAGACGCCC ACGGGAACAC AGCACTGGCC 
TATGCCCGTC AGGCCTCCAG CCAGGAGTGT ATCGACGTGC TTCTACAGTA TGGCTGCCCC 
GACGAGCGCT TTGTGCTCAT GGCTACCCCC AACCTGTCTA GAAAGAACAA CAGCAGGAAC 
AACAGTAGCG GCAGGGCGCC CAGTGTCATC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017451983.1
CDS	2464..6516
Translation	MTLQQAGPGERSIGLLREADPLRAVGIHPSAWACPPPRSPPPPRSRDPQAPLGARPAVSARRPPPARPAPARISILNRRPPAPRPTAAGATTRAARNLGRGGGSRTAELCRKRPWDALRPGALDNEAAGAAARRGLCAPRPHLLARRVPCLSAHPAPHGSALRVRTPAVGPRGTRTGGLQGRCSRSLSAGLAARGGRGRRSPPARALHLASSAPEARVPAAGGAGCSRASEGGEAAAAPGDMYLLDGSGEPASSPADTMSRGMPPRKTVYRISVTMVRKEQLAAPAPRPTRRPRPARSPEAPGRLEEEAEEAAAEDPEIGPRAWDFRAFRTRSTGQLELGRLRPCAHDTEPTDLMGSPTTEGPSSPDGEEARAAPLRRSLSFRHWSEPEVPRSPALSGGRRHSSSGSLAWMPDDDVEADVVPEPVAGVQPAAEKRNTLDVGEVLSKNDALSDLERWERSKSKNRTLDNSDLQRLERARAAAAGPGAASEHRLLRFFSGIFARRDGGPSPRSGALRSRGYFSLRRAPADTHSSSAESIEGSPRRDAFVNSQEWTLSRSVPELKVGIVGNLASGKSALVHRYLTGTYVQEESPEDMDAGGRFKKEIVVDGQSYLLLIRDEGGPPEAQFAMWVDAVIFVFSLEDEISFQTVYHYYSRMANYRNTSEIPLVLVGTQDAISSTNPRVIDDVRARKLSNDLKRCTYYETCATYGLNVERVFQDVAQKIVATRKKQQLSIGPCKSLPNSPSHSSVCSAQVSAVHISQTSNGGGSLSDYSSSVPSTPSTSQKELRIDVPPTANTPTPVRKQSKRRSNLFTSRKGSDPDKEKKGLESRADSIGSGRAIPIKQGMLLKRSGKSLNKEWKKKYVTLCDNGVLTYHPSLHDYMQNVHGKEIDLLRTTVKVPGKRPPRATSACAPISSPKTNGLAKDMSSLHISPNSGNVTSASGSQMASGISLVSFNSRPDGMHQRSYSVSSADQWSDATVIANSAISSDTGLGDSVCSSPSISSSTSPKLDPPPSPHANRKKHRRKKSTSNFKADGLTGTAEEQEENLEFIIVSLTGQTWHFEATTYEERDAWVQAIESQILASLQSCESSKNKSRLTSQSEAMALQSIRNMRGNSHCVDCDTQNPNWASLNLGALMCIECSGIHRNLGTHLSRVRSLDLDDWPMELIKVMSSIGNELANSVWEEGSQGRTKPSLDSTREEKERWIRAKYEQKLFLAPLPCTEFSLGQQLLHATAEEDLRTVILLLAHGSRDEVNETCGEGDGRTALHLACRKGNVVLVQLLIWYGVDVMARDAHGNTALAYARQASSQECIDVLLQYGCPDERFVLMATPNLSRKNNSRNNSSGRAPSVI

Target ORF information:

RefSeq Version	XM_017596494.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ArfGAP with GTPase domain, ankyrin repeat and PH domain 1 (Agap1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017596494.1

ORF Insert Sequence:

ATGACCTTGC AGCAAGCAGG TCCTGGAGAG CGCAGTATAG GATTACTGCG CGAAGCTGAT 
CCGCTCCGCG CGGTGGGGAT CCATCCCAGC GCTTGGGCTT GCCCTCCGCC GCGCTCGCCG 
CCGCCTCCGC GCTCCCGGGA CCCGCAGGCC CCGCTGGGCG CGCGGCCCGC AGTCTCCGCA 
CGTCGGCCTC CGCCCGCCCG CCCCGCCCCG GCGCGCATTT CCATTTTAAA CCGCCGCCCG 
CCCGCCCCTC GACCGACCGC GGCCGGCGCA ACCACCCGCG CGGCACGGAA CCTCGGGAGA 
GGCGGAGGCT CCCGGACTGC CGAGCTGTGC AGGAAAAGGC CCTGGGATGC GCTTCGGCCC 
GGCGCACTAG ACAATGAAGC CGCGGGCGCT GCTGCCCGCC GCGGGCTTTG CGCACCCAGG 
CCGCACCTGC TGGCGAGGCG GGTTCCGTGC CTGAGCGCCC ACCCAGCCCC TCATGGCTCG 
GCTCTCCGGG TGCGGACCCC GGCAGTCGGG CCGCGTGGCA CGCGGACGGG CGGACTCCAG 
GGGCGCTGTT CCCGCAGCCT GAGCGCGGGC CTCGCTGCGC GCGGGGGGCG TGGGCGCCGC 
TCCCCGCCCG CTCGGGCTCT GCACCTGGCC AGCTCTGCGC CTGAGGCGCG CGTCCCGGCA 
GCCGGCGGCG CTGGATGCAG CCGGGCCTCG GAGGGCGGCG AGGCGGCCGC GGCCCCTGGG 
GACATGTACC TGCTGGACGG CAGCGGCGAG CCCGCGTCTT CGCCTGCGGA CACGATGTCG 
CGCGGGATGC CCCCCAGGAA GACGGTCTAC CGCATCTCGG TGACCATGGT CAGGAAGGAG 
CAGCTGGCCG CTCCGGCTCC ACGTCCTACT CGGCGGCCCC GGCCCGCGCG CTCCCCGGAG 
GCGCCCGGGC GGCTGGAGGA GGAGGCCGAG GAGGCGGCGG CTGAGGATCC TGAGATCGGG 
CCGCGCGCCT GGGACTTCCG CGCCTTTCGC ACACGCAGCA CCGGGCAGCT GGAGCTCGGG 
CGGCTGCGGC CGTGCGCACA CGACACAGAA CCCACCGACC TGATGGGTAG CCCCACGACT 
GAGGGGCCCA GCAGTCCTGA TGGGGAGGAG GCACGGGCCG CGCCCCTGCG GCGGAGCCTC 
AGCTTTAGGC ACTGGAGCGA GCCCGAGGTG CCACGGAGCC CTGCGCTGAG CGGCGGGAGG 
CGCCACAGCA GCTCTGGAAG CCTGGCATGG ATGCCCGACG ACGATGTCGA GGCGGACGTC 
GTTCCGGAAC CGGTGGCTGG AGTGCAGCCG GCTGCTGAGA AGCGCAACAC CTTGGACGTG 
GGCGAGGTGC TTAGCAAGAA CGATGCCCTC TCAGACCTGG AGCGCTGGGA GCGCAGCAAG 
AGCAAGAACC GCACGCTGGA CAACAGCGAC CTGCAGCGGC TGGAGCGCGC GCGGGCCGCG 
GCTGCCGGCC CTGGGGCCGC CTCGGAGCAC CGGCTGCTGC GTTTCTTCAG TGGCATCTTC 
GCGCGCAGGG ACGGCGGGCC CTCACCCCGC AGCGGCGCCC TACGCTCGCG CGGTTACTTC 
AGCCTGCGGC GGGCCCCGGC CGACACGCAT TCGTCCAGTG CCGAGAGCAT CGAGGGGTCC 
CCGCGCAGAG ATGCTTTCGT GAACAGCCAG GAATGGACAC TCAGCCGATC AGTACCAGAG 
CTCAAAGTGG GAATTGTGGG TAACCTGGCC AGTGGCAAGT CTGCGCTGGT GCACCGGTAC 
CTGACAGGCA CATACGTGCA GGAGGAGTCT CCGGAAGATA TGGACGCAGG TGGCAGGTTC 
AAGAAGGAGA TTGTTGTTGA TGGACAGAGT TATCTGCTGC TGATTAGGGA TGAAGGGGGT 
CCCCCGGAGG CACAGTTTGC CATGTGGGTG GACGCGGTCA TCTTTGTCTT CAGCTTGGAG 
GATGAGATCA GTTTCCAGAC CGTCTACCAT TACTACAGCC GAATGGCCAA CTACAGGAAC 
ACCAGTGAGA TCCCATTAGT GCTGGTGGGG ACCCAGGACG CCATAAGTTC CACCAACCCG 
AGGGTCATCG ATGACGTCAG GGCTCGGAAG CTCTCCAATG ACCTGAAGCG GTGCACGTAC 
TATGAGACGT GCGCCACCTA TGGGCTCAAC GTGGAGCGGG TCTTCCAGGA CGTTGCCCAG 
AAGATTGTTG CCACGAGGAA GAAGCAGCAG CTGTCCATAG GCCCCTGCAA GTCCTTACCC 
AACTCTCCAA GCCACTCCTC TGTGTGTTCT GCCCAAGTGT CCGCAGTACA CATCAGCCAG 
ACAAGTAACG GAGGTGGGAG TTTAAGCGAC TACTCATCCT CTGTCCCTTC GACCCCAAGC 
ACCAGCCAGA AGGAGCTTCG GATCGACGTC CCTCCCACTG CCAACACTCC AACGCCCGTC 
CGGAAGCAAT CCAAGCGCAG GTCCAACCTA TTCACATCTA GGAAAGGGAG CGACCCAGAC 
AAAGAGAAGA AAGGCCTGGA GAGCCGCGCG GACAGCATCG GGAGCGGGCG AGCCATCCCA 
ATTAAACAGG GCATGCTCCT GAAAAGAAGT GGCAAGTCAC TAAACAAAGA GTGGAAAAAG 
AAGTACGTCA CCCTATGCGA CAATGGTGTG CTGACCTACC ACCCCAGTTT ACATGACTAC 
ATGCAGAACG TCCATGGCAA AGAGATAGAC CTCTTGAGAA CCACTGTGAA GGTGCCGGGG 
AAGAGGCCGC CCCGAGCCAC ATCAGCCTGT GCGCCGATCT CCAGTCCCAA AACCAATGGC 
CTGGCCAAGG ACATGAGCAG TTTACACATC TCACCAAATT CAGGGAATGT CACTAGTGCG 
TCTGGGTCTC AGATGGCAAG CGGCATCAGC CTGGTCTCCT TCAACAGCCG ACCCGATGGC 
ATGCACCAGC GCTCCTACTC AGTCTCCAGT GCCGACCAGT GGAGTGACGC TACAGTCATT 
GCAAACTCAG CCATCAGCAG TGACACGGGG CTGGGTGACT CGGTGTGTTC CAGCCCAAGT 
ATCTCCAGCA GCACCAGCCC CAAGCTCGAC CCACCCCCCT CCCCCCACGC CAACAGAAAG 
AAGCATCGGA GGAAGAAAAG TACCAGCAAC TTCAAGGCCG ACGGGCTCAC GGGCACCGCT 
GAAGAGCAAG AAGAAAACTT GGAGTTTATC ATTGTGTCCC TCACTGGCCA GACGTGGCAC 
TTTGAAGCCA CCACATACGA GGAGCGAGAC GCATGGGTCC AAGCCATCGA GAGCCAGATC 
CTAGCCAGCC TGCAGTCCTG TGAGAGCAGC AAGAATAAGT CCCGACTCAC CAGCCAGAGC 
GAAGCCATGG CACTGCAGTC AATCCGTAAC ATGAGAGGGA ACTCGCACTG CGTGGACTGT 
GACACCCAGA ACCCCAACTG GGCCAGTTTG AACTTGGGAG CCCTCATGTG TATCGAGTGC 
TCAGGGATCC ACCGGAATCT CGGCACCCAC CTCTCTCGGG TCCGATCGCT CGACCTCGAC 
GACTGGCCCA TGGAGCTGAT CAAGGTGATG TCCTCCATCG GGAATGAGCT GGCCAACAGC 
GTGTGGGAAG AGGGCAGCCA GGGACGGACA AAGCCCTCGT TGGACTCCAC CAGGGAAGAA 
AAGGAACGGT GGATTCGGGC CAAGTATGAG CAGAAACTCT TCCTGGCCCC ACTGCCATGC 
ACAGAGTTCT CCCTGGGCCA GCAACTGCTA CATGCCACTG CTGAGGAAGA CCTGCGGACT 
GTCATCCTGC TTCTGGCACA CGGGTCACGG GATGAGGTGA ATGAGACCTG TGGGGAAGGA 
GATGGTCGCA CGGCCCTGCA CCTGGCCTGC CGCAAGGGCA ACGTGGTCCT GGTTCAGCTG 
CTGATCTGGT ACGGGGTGGA CGTCATGGCC CGAGACGCCC ACGGGAACAC AGCACTGGCC 
TATGCCCGTC AGGCCTCCAG CCAGGAGTGT ATCGACGTGC TTCTACAGTA TGGCTGCCCC 
GACGAGCGCT TTGTGCTCAT GGCTACCCCC AACCTGTCTA GAAAGAACAA CAGCAGGAAC 
AACAGTAGCG GCAGGGCGCC CAGTGTCATC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	Agap1
Entrez Gene ID	316611
Full Name	ArfGAP with GTPase domain, ankyrin repeat and PH domain 1
Synonyms	Centg2
General protein information	Preferred Names ArfGAP with GTPase domain, ankyrin repeat and PH domain 1 Names arf-GAP with GTPase, ANK repeat and PH domain-containing protein 1 centaurin, gamma 2
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 9 Map Location: 9q35-q36

Mus musculus (house mouse)	Agap1	NP_835220.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009160	XP_319921.4
Xenopus tropicalis (tropical clawed frog)	agap1	XP_002932044.1
Caenorhabditis elegans (roundworm)	cnt-2	NP_001022837.1
Gallus gallus (chicken)	AGAP1	XP_004942549.1
Drosophila melanogaster (fruit fly)	cenG1A	NP_723849.1
Canis lupus familiaris (dog)	AGAP1	XP_005635967.1
Homo sapiens (human)	AGAP1	NP_001032208.1
	AGAP1	XP_001082607.1
Rattus norvegicus (Norway rat)	Agap1	NP_001101700.1
Danio rerio (zebrafish)	agap1	XP_001921526.3

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

Agap1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

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