Pomgnt2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Pomgnt2
Entrez Gene ID	316091
Full Name	protein O-linked mannose N-acetylglucosaminyltransferase 2 (beta 1,4-)
Synonyms	Ago61,Gtdc2,RGD1304827
General protein information	Preferred Names protein O-linked mannose N-acetylglucosaminyltransferase 2 (beta 1,4-) Names protein O-linked-mannose beta-1,4-N-acetylglucosaminyltransferase 2 glycosyltransferase glycosyltransferase-like domain containing 2 glycosyltransferase-like domain-containing protein 2
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 8 Map Location: 8q32

mRNA and Protein(s)

mRNA	Protein	Name
XM_008766681.2	XP_008764903.1	protein O-linked-mannose beta-1,4-N-acetylglucosaminyltransferase 2 isoform X1
XM_008766683.2	XP_008764905.1	protein O-linked-mannose beta-1,4-N-acetylglucosaminyltransferase 2 isoform X1
NM_001009437.1	NP_001009437.1	protein O-linked-mannose beta-1,4-N-acetylglucosaminyltransferase 2 precursor

Pathways from BioSystems

Homologies

Homo sapiens (human)	POMGNT2	NP_116195.2
Canis lupus familiaris (dog)	GTDC2	NP_001010957.1
	LOC717032	XP_001108195.1
Mus musculus (house mouse)	Pomgnt2	NP_705768.4
Danio rerio (zebrafish)	pomgnt2	NP_001012384.1
Bos taurus (cattle)	GTDC2	NP_001011682.1
Gallus gallus (chicken)	C2H3ORF39	NP_001012294.1
Pan troglodytes (chimpanzee)	GTDC2	NP_001012742.1
Rattus norvegicus (Norway rat)	Pomgnt2	NP_001009437.1
Xenopus tropicalis (tropical clawed frog)	pomgnt2	NP_001011203.1

Gene Ontology
Bibliography

Related articles in PubMed

Milder forms of muscular dystrophy associated with POMGNT2 mutations.
Endo Y, Dong M, Noguchi S, Ogawa M, Hayashi YK, Kuru S, Sugiyama K, Nagai S, Ozasa S, Nonaka I, Nishino I
Neurology. Genetics1(4)e33(2015 Dec)

Ectopic clustering of Cajal-Retzius and subplate cells is an initial pathological feature in Pomgnt2-knockout mice, a model of dystroglycanopathy.
Nakagawa N, Yagi H, Kato K, Takematsu H, Oka S
Scientific reports511163(2015 Jun)

AGO61-dependent GlcNAc modification primes the formation of functional glycans on ?-dystroglycan.
Yagi H, Nakagawa N, Saito T, Kiyonari H, Abe T, Toda T, Wu SW, Khoo KH, Oka S, Kato K
Scientific reports33288(2013 Nov)

SGK196 is a glycosylation-specific O-mannose kinase required for dystroglycan function.
Yoshida-Moriguchi T, Willer T, Anderson ME, Venzke D, Whyte T, Muntoni F, Lee H, Nelson SF, Yu L, Campbell KP
Science (New York, N.Y.)341(6148)896-9(2013 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Pomgnt2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pomgnt2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa13930	NM_001009437.1 Latest version!	Rattus norvegicus protein O-linked mannose N-acetylglucosaminyltransferase 2 (beta 1,4-) (Pomgnt2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
ORa13930	XM_008766681.2 Latest version!	Rattus norvegicus protein O-linked mannose N-acetylglucosaminyltransferase 2 (beta 1,4-) (Pomgnt2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
ORa13930	XM_008766683.2 Latest version!	Rattus norvegicus protein O-linked mannose N-acetylglucosaminyltransferase 2 (beta 1,4-) (Pomgnt2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Pomgnt2 ( NM_001009437.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001009437.1 Rattus norvegicus protein O-linked mannose N-acetylglucosaminyltransferase 2 (beta 1,4-) (Pomgnt2), mRNA.

Price & Availability↑

CloneID	ORa13930
Clone ID Related Accession (Same CDS sequence)	XM_008766681.2 , XM_008766683.2 , NM_001009437.1
Accession Version	NM_001009437.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1743bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-09
Organism	Rattus norvegicus(Norway rat)
Product	protein O-linked-mannose beta-1,4-N-acetylglucosaminyltransferase 2 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AJ868534.1. On Jan 12, 2005 this sequence version replaced XM_236725.2. ##Evidence-Data-START## Transcript exon combination :: AJ868534.1 [ECO:0000332] ##Evidence-Data-END##

ATGCACCTCT CTGCAGTATT CAACGCCCTC CTGGTATCAG TGCTGGCTGC GGTCCTGTGG 
AAGCACGTGC GGTTGCGGGA GCATGCGGCC ACGCTAGAGG AGGAGCTGGC CCTTGGACAG 
CAGTCCCTGG ATCCAGTCCC AGGGCTGAGG ATCGACTACC CGAAGGCCCT GCAGATCCTC 
ATGGAGGGTG GCACCCACAT GGTGTGCACG GGCCGCACCC ACACGGACCG CATCTGCCGC 
TTCAAGTGGC TCTGCTACTC CAATGAGGCC GAAGAATTCA TCTTTTTCCA CGGCAACTCG 
TCTGTGATGC TGCCCAACCT GGGCTCCCGG CGCTTCCAGC CGGCCCTGCT GGACCTGTCC 
ACTGTGGAGG ACCATAATGC CCAGTACTTC AACTTCGTGG AGCTGCCTGC CGCAGCCCTG 
CGCTTCATGC CAAAGCCAGT GTTCGTGCCT GATGTGGCCC TCATCGCCAA CCGCTTCAAC 
CCCGATAACC TCATGCACGT CTTCCATGAT GACCTGCTCC CCCTCTTCTA CACACTGAGG 
CAGTTCCCCG GCCTGGCCCA AGAGGCCCGG CTCTTCTTTA TGGAGGGGTG GGGCGAGGGC 
GCCCACTTTG ACCTCTATAA ACTCCTCAGC CCCAAGCAGC CGCTGCTCCG TTCGCAGCTC 
AAGACCCTGG GCCGGCTGCT CTGCTTTTCC CATGCTTTCG TGGGCCTGTC CAAGGTCACC 
ACCTGGTACC AGTACGGCTT TGTCCAGCCC CAGGGCCCAA AGGCCAACAT CCTGGTCTCT 
GGCAATGAGA TCCGGCAGTT CACCCGCTTC ATGACTGAAA GGCTGAACGT GAGCCACGCG 
GGGGCGCCCC TCGGGGAGGA ATACATTCTG GTCTTCAGCC GCACCCAGAA TAGACTCATC 
CTGAATGAGG CAGAGCTGTT GCTGGAGCTA GCACAAGAGT TCCAGATGAA GACCGTGACA 
GTGTCCCTGG AGGACCACAC CTTTGCCGAT GTCGTGCGGC TGGTCAGCAA CGCCTCCATG 
TTAGTCAGCA TGCATGGGGC CCAGCTGGTG ACAGCCCTCT TCCTGCCCCG TGGGGCTACT 
GTGGTTGAAC TCTTCCCGTA CGCTGTCAAT CCTGACCACT ACACCCCCTA TAAGACGCTG 
GCCACACTGC CTGGCATGGA CCTGCAGTAT GTAGCCTGGA GAAACATGAT CCGGGAGAAC 
ACAGTCACGC ACCCCGAGCG ACCCTGGGAC CAAGGGGGAA TCACCCACTT GGACCGGGCT 
GAGCAGGCCC GCATCCTACA GAGCCGAGAG GTTCCCCGGC ATCTGTGTTG CCGGAACCCA 
GAGTGGCTAT TCCGAATCTA CCAGGACACC AGGGTGGACA TCCCATCCCT CATGCAATCC 
ATTCGGCGTG TGGTGAAGGG CCGGCCGGGG CCACGGAGGC AGAGGTGGGC AATCAGCCTG 
TACCCTGGTA AGGTACGGGA GGCTCGATGT CAGGCGTCGG TGCAGGGCGC CACGGAGGCC 
CGCCTGTCTG TGTCCTGGCA GATCCCGTGG AACCTCAAGT ACCTGAAAGT GAGGGAGGTG 
AGGTACGAGG TGTGGCTGCA GGAGCAAGGG GAGAACACGT ATGTGCCTTA CATGCTGACG 
CTGCAGAACC ATACCTTCAC GGAGAACATC AAACCTTTTA CCACCTACCT GGTGTGGGTC 
CGCTGCATCT TCAACAGGAG CCTCCTGGGA CCTTTTGCAG ATGTGCTGGT GTGCAGCACG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001009437.1
CDS	1..1743
Translation	MHLSAVFNALLVSVLAAVLWKHVRLREHAATLEEELALGQQSLDPVPGLRIDYPKALQILMEGGTHMVCTGRTHTDRICRFKWLCYSNEAEEFIFFHGNSSVMLPNLGSRRFQPALLDLSTVEDHNAQYFNFVELPAAALRFMPKPVFVPDVALIANRFNPDNLMHVFHDDLLPLFYTLRQFPGLAQEARLFFMEGWGEGAHFDLYKLLSPKQPLLRSQLKTLGRLLCFSHAFVGLSKVTTWYQYGFVQPQGPKANILVSGNEIRQFTRFMTERLNVSHAGAPLGEEYILVFSRTQNRLILNEAELLLELAQEFQMKTVTVSLEDHTFADVVRLVSNASMLVSMHGAQLVTALFLPRGATVVELFPYAVNPDHYTPYKTLATLPGMDLQYVAWRNMIRENTVTHPERPWDQGGITHLDRAEQARILQSREVPRHLCCRNPEWLFRIYQDTRVDIPSLMQSIRRVVKGRPGPRRQRWAISLYPGKVREARCQASVQGATEARLSVSWQIPWNLKYLKVREVRYEVWLQEQGENTYVPYMLTLQNHTFTENIKPFTTYLVWVRCIFNRSLLGPFADVLVCST

Target ORF information:

RefSeq Version	NM_001009437.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus protein O-linked mannose N-acetylglucosaminyltransferase 2 (beta 1,4-) (Pomgnt2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001009437.1

ORF Insert Sequence:

ATGCACCTCT CTGCAGTATT CAACGCCCTC CTGGTATCAG TGCTGGCTGC GGTCCTGTGG 
AAGCACGTGC GGTTGCGGGA GCATGCGGCC ACGCTAGAGG AGGAGCTGGC CCTTGGACAG 
CAGTCCCTGG ATCCAGTCCC AGGGCTGAGG ATCGACTACC CGAAGGCCCT GCAGATCCTC 
ATGGAGGGTG GCACCCACAT GGTGTGCACG GGCCGCACCC ACACGGACCG CATCTGCCGC 
TTCAAGTGGC TCTGCTACTC CAATGAGGCC GAAGAATTCA TCTTTTTCCA CGGCAACTCG 
TCTGTGATGC TGCCCAACCT GGGCTCCCGG CGCTTCCAGC CGGCCCTGCT GGACCTGTCC 
ACTGTGGAGG ACCATAATGC CCAGTACTTC AACTTCGTGG AGCTGCCTGC CGCAGCCCTG 
CGCTTCATGC CAAAGCCAGT GTTCGTGCCT GATGTGGCCC TCATCGCCAA CCGCTTCAAC 
CCCGATAACC TCATGCACGT CTTCCATGAT GACCTGCTCC CCCTCTTCTA CACACTGAGG 
CAGTTCCCCG GCCTGGCCCA AGAGGCCCGG CTCTTCTTTA TGGAGGGGTG GGGCGAGGGC 
GCCCACTTTG ACCTCTATAA ACTCCTCAGC CCCAAGCAGC CGCTGCTCCG TTCGCAGCTC 
AAGACCCTGG GCCGGCTGCT CTGCTTTTCC CATGCTTTCG TGGGCCTGTC CAAGGTCACC 
ACCTGGTACC AGTACGGCTT TGTCCAGCCC CAGGGCCCAA AGGCCAACAT CCTGGTCTCT 
GGCAATGAGA TCCGGCAGTT CACCCGCTTC ATGACTGAAA GGCTGAACGT GAGCCACGCG 
GGGGCGCCCC TCGGGGAGGA ATACATTCTG GTCTTCAGCC GCACCCAGAA TAGACTCATC 
CTGAATGAGG CAGAGCTGTT GCTGGAGCTA GCACAAGAGT TCCAGATGAA GACCGTGACA 
GTGTCCCTGG AGGACCACAC CTTTGCCGAT GTCGTGCGGC TGGTCAGCAA CGCCTCCATG 
TTAGTCAGCA TGCATGGGGC CCAGCTGGTG ACAGCCCTCT TCCTGCCCCG TGGGGCTACT 
GTGGTTGAAC TCTTCCCGTA CGCTGTCAAT CCTGACCACT ACACCCCCTA TAAGACGCTG 
GCCACACTGC CTGGCATGGA CCTGCAGTAT GTAGCCTGGA GAAACATGAT CCGGGAGAAC 
ACAGTCACGC ACCCCGAGCG ACCCTGGGAC CAAGGGGGAA TCACCCACTT GGACCGGGCT 
GAGCAGGCCC GCATCCTACA GAGCCGAGAG GTTCCCCGGC ATCTGTGTTG CCGGAACCCA 
GAGTGGCTAT TCCGAATCTA CCAGGACACC AGGGTGGACA TCCCATCCCT CATGCAATCC 
ATTCGGCGTG TGGTGAAGGG CCGGCCGGGG CCACGGAGGC AGAGGTGGGC AATCAGCCTG 
TACCCTGGTA AGGTACGGGA GGCTCGATGT CAGGCGTCGG TGCAGGGCGC CACGGAGGCC 
CGCCTGTCTG TGTCCTGGCA GATCCCGTGG AACCTCAAGT ACCTGAAAGT GAGGGAGGTG 
AGGTACGAGG TGTGGCTGCA GGAGCAAGGG GAGAACACGT ATGTGCCTTA CATGCTGACG 
CTGCAGAACC ATACCTTCAC GGAGAACATC AAACCTTTTA CCACCTACCT GGTGTGGGTC 
CGCTGCATCT TCAACAGGAG CCTCCTGGGA CCTTTTGCAG ATGTGCTGGT GTGCAGCACG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pomgnt2 ( XM_008766681.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008764903.1 Rattus norvegicus protein O-linked mannose N-acetylglucosaminyltransferase 2 (beta 1,4-) (Pomgnt2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa13930
Clone ID Related Accession (Same CDS sequence)	XM_008766681.2 , XM_008766683.2 , NM_001009437.1
Accession Version	XM_008766681.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1743bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	protein O-linked-mannose beta-1,4-N-acetylglucosaminyltransferase 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005107.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008766681.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACCTCT CTGCAGTATT CAACGCCCTC CTGGTATCAG TGCTGGCTGC GGTCCTGTGG 
AAGCACGTGC GGTTGCGGGA GCATGCGGCC ACGCTAGAGG AGGAGCTGGC CCTTGGACAG 
CAGTCCCTGG ATCCAGTCCC AGGGCTGAGG ATCGACTACC CGAAGGCCCT GCAGATCCTC 
ATGGAGGGTG GCACCCACAT GGTGTGCACG GGCCGCACCC ACACGGACCG CATCTGCCGC 
TTCAAGTGGC TCTGCTACTC CAATGAGGCC GAAGAATTCA TCTTTTTCCA CGGCAACTCG 
TCTGTGATGC TGCCCAACCT GGGCTCCCGG CGCTTCCAGC CGGCCCTGCT GGACCTGTCC 
ACTGTGGAGG ACCATAATGC CCAGTACTTC AACTTCGTGG AGCTGCCTGC CGCAGCCCTG 
CGCTTCATGC CAAAGCCAGT GTTCGTGCCT GATGTGGCCC TCATCGCCAA CCGCTTCAAC 
CCCGATAACC TCATGCACGT CTTCCATGAT GACCTGCTCC CCCTCTTCTA CACACTGAGG 
CAGTTCCCCG GCCTGGCCCA AGAGGCCCGG CTCTTCTTTA TGGAGGGGTG GGGCGAGGGC 
GCCCACTTTG ACCTCTATAA ACTCCTCAGC CCCAAGCAGC CGCTGCTCCG TTCGCAGCTC 
AAGACCCTGG GCCGGCTGCT CTGCTTTTCC CATGCTTTCG TGGGCCTGTC CAAGGTCACC 
ACCTGGTACC AGTACGGCTT TGTCCAGCCC CAGGGCCCAA AGGCCAACAT CCTGGTCTCT 
GGCAATGAGA TCCGGCAGTT CACCCGCTTC ATGACTGAAA GGCTGAACGT GAGCCACGCG 
GGGGCGCCCC TCGGGGAGGA ATACATTCTG GTCTTCAGCC GCACCCAGAA TAGACTCATC 
CTGAATGAGG CAGAGCTGTT GCTGGAGCTA GCACAAGAGT TCCAGATGAA GACCGTGACA 
GTGTCCCTGG AGGACCACAC CTTTGCCGAT GTCGTGCGGC TGGTCAGCAA CGCCTCCATG 
TTAGTCAGCA TGCATGGGGC CCAGCTGGTG ACAGCCCTCT TCCTGCCCCG TGGGGCTACT 
GTGGTTGAAC TCTTCCCGTA CGCTGTCAAT CCTGACCACT ACACCCCCTA TAAGACGCTG 
GCCACACTGC CTGGCATGGA CCTGCAGTAT GTAGCCTGGA GAAACATGAT CCGGGAGAAC 
ACAGTCACGC ACCCCGAGCG ACCCTGGGAC CAAGGGGGAA TCACCCACTT GGACCGGGCT 
GAGCAGGCCC GCATCCTACA GAGCCGAGAG GTTCCCCGGC ATCTGTGTTG CCGGAACCCA 
GAGTGGCTAT TCCGAATCTA CCAGGACACC AGGGTGGACA TCCCATCCCT CATGCAATCC 
ATTCGGCGTG TGGTGAAGGG CCGGCCGGGG CCACGGAGGC AGAGGTGGGC AATCAGCCTG 
TACCCTGGTA AGGTACGGGA GGCTCGATGT CAGGCGTCGG TGCAGGGCGC CACGGAGGCC 
CGCCTGTCTG TGTCCTGGCA GATCCCGTGG AACCTCAAGT ACCTGAAAGT GAGGGAGGTG 
AGGTACGAGG TGTGGCTGCA GGAGCAAGGG GAGAACACGT ATGTGCCTTA CATGCTGACG 
CTGCAGAACC ATACCTTCAC GGAGAACATC AAACCTTTTA CCACCTACCT GGTGTGGGTC 
CGCTGCATCT TCAACAGGAG CCTCCTGGGA CCTTTTGCAG ATGTGCTGGT GTGCAGCACG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008764903.1
CDS	275..2017
Translation	MHLSAVFNALLVSVLAAVLWKHVRLREHAATLEEELALGQQSLDPVPGLRIDYPKALQILMEGGTHMVCTGRTHTDRICRFKWLCYSNEAEEFIFFHGNSSVMLPNLGSRRFQPALLDLSTVEDHNAQYFNFVELPAAALRFMPKPVFVPDVALIANRFNPDNLMHVFHDDLLPLFYTLRQFPGLAQEARLFFMEGWGEGAHFDLYKLLSPKQPLLRSQLKTLGRLLCFSHAFVGLSKVTTWYQYGFVQPQGPKANILVSGNEIRQFTRFMTERLNVSHAGAPLGEEYILVFSRTQNRLILNEAELLLELAQEFQMKTVTVSLEDHTFADVVRLVSNASMLVSMHGAQLVTALFLPRGATVVELFPYAVNPDHYTPYKTLATLPGMDLQYVAWRNMIRENTVTHPERPWDQGGITHLDRAEQARILQSREVPRHLCCRNPEWLFRIYQDTRVDIPSLMQSIRRVVKGRPGPRRQRWAISLYPGKVREARCQASVQGATEARLSVSWQIPWNLKYLKVREVRYEVWLQEQGENTYVPYMLTLQNHTFTENIKPFTTYLVWVRCIFNRSLLGPFADVLVCST

Target ORF information:

RefSeq Version	XM_008766681.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus protein O-linked mannose N-acetylglucosaminyltransferase 2 (beta 1,4-) (Pomgnt2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008766681.2

ORF Insert Sequence:

ATGCACCTCT CTGCAGTATT CAACGCCCTC CTGGTATCAG TGCTGGCTGC GGTCCTGTGG 
AAGCACGTGC GGTTGCGGGA GCATGCGGCC ACGCTAGAGG AGGAGCTGGC CCTTGGACAG 
CAGTCCCTGG ATCCAGTCCC AGGGCTGAGG ATCGACTACC CGAAGGCCCT GCAGATCCTC 
ATGGAGGGTG GCACCCACAT GGTGTGCACG GGCCGCACCC ACACGGACCG CATCTGCCGC 
TTCAAGTGGC TCTGCTACTC CAATGAGGCC GAAGAATTCA TCTTTTTCCA CGGCAACTCG 
TCTGTGATGC TGCCCAACCT GGGCTCCCGG CGCTTCCAGC CGGCCCTGCT GGACCTGTCC 
ACTGTGGAGG ACCATAATGC CCAGTACTTC AACTTCGTGG AGCTGCCTGC CGCAGCCCTG 
CGCTTCATGC CAAAGCCAGT GTTCGTGCCT GATGTGGCCC TCATCGCCAA CCGCTTCAAC 
CCCGATAACC TCATGCACGT CTTCCATGAT GACCTGCTCC CCCTCTTCTA CACACTGAGG 
CAGTTCCCCG GCCTGGCCCA AGAGGCCCGG CTCTTCTTTA TGGAGGGGTG GGGCGAGGGC 
GCCCACTTTG ACCTCTATAA ACTCCTCAGC CCCAAGCAGC CGCTGCTCCG TTCGCAGCTC 
AAGACCCTGG GCCGGCTGCT CTGCTTTTCC CATGCTTTCG TGGGCCTGTC CAAGGTCACC 
ACCTGGTACC AGTACGGCTT TGTCCAGCCC CAGGGCCCAA AGGCCAACAT CCTGGTCTCT 
GGCAATGAGA TCCGGCAGTT CACCCGCTTC ATGACTGAAA GGCTGAACGT GAGCCACGCG 
GGGGCGCCCC TCGGGGAGGA ATACATTCTG GTCTTCAGCC GCACCCAGAA TAGACTCATC 
CTGAATGAGG CAGAGCTGTT GCTGGAGCTA GCACAAGAGT TCCAGATGAA GACCGTGACA 
GTGTCCCTGG AGGACCACAC CTTTGCCGAT GTCGTGCGGC TGGTCAGCAA CGCCTCCATG 
TTAGTCAGCA TGCATGGGGC CCAGCTGGTG ACAGCCCTCT TCCTGCCCCG TGGGGCTACT 
GTGGTTGAAC TCTTCCCGTA CGCTGTCAAT CCTGACCACT ACACCCCCTA TAAGACGCTG 
GCCACACTGC CTGGCATGGA CCTGCAGTAT GTAGCCTGGA GAAACATGAT CCGGGAGAAC 
ACAGTCACGC ACCCCGAGCG ACCCTGGGAC CAAGGGGGAA TCACCCACTT GGACCGGGCT 
GAGCAGGCCC GCATCCTACA GAGCCGAGAG GTTCCCCGGC ATCTGTGTTG CCGGAACCCA 
GAGTGGCTAT TCCGAATCTA CCAGGACACC AGGGTGGACA TCCCATCCCT CATGCAATCC 
ATTCGGCGTG TGGTGAAGGG CCGGCCGGGG CCACGGAGGC AGAGGTGGGC AATCAGCCTG 
TACCCTGGTA AGGTACGGGA GGCTCGATGT CAGGCGTCGG TGCAGGGCGC CACGGAGGCC 
CGCCTGTCTG TGTCCTGGCA GATCCCGTGG AACCTCAAGT ACCTGAAAGT GAGGGAGGTG 
AGGTACGAGG TGTGGCTGCA GGAGCAAGGG GAGAACACGT ATGTGCCTTA CATGCTGACG 
CTGCAGAACC ATACCTTCAC GGAGAACATC AAACCTTTTA CCACCTACCT GGTGTGGGTC 
CGCTGCATCT TCAACAGGAG CCTCCTGGGA CCTTTTGCAG ATGTGCTGGT GTGCAGCACG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pomgnt2 ( XM_008766683.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008764905.1 Rattus norvegicus protein O-linked mannose N-acetylglucosaminyltransferase 2 (beta 1,4-) (Pomgnt2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa13930
Clone ID Related Accession (Same CDS sequence)	XM_008766681.2 , XM_008766683.2 , NM_001009437.1
Accession Version	XM_008766683.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1743bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	protein O-linked-mannose beta-1,4-N-acetylglucosaminyltransferase 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005107.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008766683.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCACCTCT CTGCAGTATT CAACGCCCTC CTGGTATCAG TGCTGGCTGC GGTCCTGTGG 
AAGCACGTGC GGTTGCGGGA GCATGCGGCC ACGCTAGAGG AGGAGCTGGC CCTTGGACAG 
CAGTCCCTGG ATCCAGTCCC AGGGCTGAGG ATCGACTACC CGAAGGCCCT GCAGATCCTC 
ATGGAGGGTG GCACCCACAT GGTGTGCACG GGCCGCACCC ACACGGACCG CATCTGCCGC 
TTCAAGTGGC TCTGCTACTC CAATGAGGCC GAAGAATTCA TCTTTTTCCA CGGCAACTCG 
TCTGTGATGC TGCCCAACCT GGGCTCCCGG CGCTTCCAGC CGGCCCTGCT GGACCTGTCC 
ACTGTGGAGG ACCATAATGC CCAGTACTTC AACTTCGTGG AGCTGCCTGC CGCAGCCCTG 
CGCTTCATGC CAAAGCCAGT GTTCGTGCCT GATGTGGCCC TCATCGCCAA CCGCTTCAAC 
CCCGATAACC TCATGCACGT CTTCCATGAT GACCTGCTCC CCCTCTTCTA CACACTGAGG 
CAGTTCCCCG GCCTGGCCCA AGAGGCCCGG CTCTTCTTTA TGGAGGGGTG GGGCGAGGGC 
GCCCACTTTG ACCTCTATAA ACTCCTCAGC CCCAAGCAGC CGCTGCTCCG TTCGCAGCTC 
AAGACCCTGG GCCGGCTGCT CTGCTTTTCC CATGCTTTCG TGGGCCTGTC CAAGGTCACC 
ACCTGGTACC AGTACGGCTT TGTCCAGCCC CAGGGCCCAA AGGCCAACAT CCTGGTCTCT 
GGCAATGAGA TCCGGCAGTT CACCCGCTTC ATGACTGAAA GGCTGAACGT GAGCCACGCG 
GGGGCGCCCC TCGGGGAGGA ATACATTCTG GTCTTCAGCC GCACCCAGAA TAGACTCATC 
CTGAATGAGG CAGAGCTGTT GCTGGAGCTA GCACAAGAGT TCCAGATGAA GACCGTGACA 
GTGTCCCTGG AGGACCACAC CTTTGCCGAT GTCGTGCGGC TGGTCAGCAA CGCCTCCATG 
TTAGTCAGCA TGCATGGGGC CCAGCTGGTG ACAGCCCTCT TCCTGCCCCG TGGGGCTACT 
GTGGTTGAAC TCTTCCCGTA CGCTGTCAAT CCTGACCACT ACACCCCCTA TAAGACGCTG 
GCCACACTGC CTGGCATGGA CCTGCAGTAT GTAGCCTGGA GAAACATGAT CCGGGAGAAC 
ACAGTCACGC ACCCCGAGCG ACCCTGGGAC CAAGGGGGAA TCACCCACTT GGACCGGGCT 
GAGCAGGCCC GCATCCTACA GAGCCGAGAG GTTCCCCGGC ATCTGTGTTG CCGGAACCCA 
GAGTGGCTAT TCCGAATCTA CCAGGACACC AGGGTGGACA TCCCATCCCT CATGCAATCC 
ATTCGGCGTG TGGTGAAGGG CCGGCCGGGG CCACGGAGGC AGAGGTGGGC AATCAGCCTG 
TACCCTGGTA AGGTACGGGA GGCTCGATGT CAGGCGTCGG TGCAGGGCGC CACGGAGGCC 
CGCCTGTCTG TGTCCTGGCA GATCCCGTGG AACCTCAAGT ACCTGAAAGT GAGGGAGGTG 
AGGTACGAGG TGTGGCTGCA GGAGCAAGGG GAGAACACGT ATGTGCCTTA CATGCTGACG 
CTGCAGAACC ATACCTTCAC GGAGAACATC AAACCTTTTA CCACCTACCT GGTGTGGGTC 
CGCTGCATCT TCAACAGGAG CCTCCTGGGA CCTTTTGCAG ATGTGCTGGT GTGCAGCACG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008764905.1
CDS	345..2087
Translation	MHLSAVFNALLVSVLAAVLWKHVRLREHAATLEEELALGQQSLDPVPGLRIDYPKALQILMEGGTHMVCTGRTHTDRICRFKWLCYSNEAEEFIFFHGNSSVMLPNLGSRRFQPALLDLSTVEDHNAQYFNFVELPAAALRFMPKPVFVPDVALIANRFNPDNLMHVFHDDLLPLFYTLRQFPGLAQEARLFFMEGWGEGAHFDLYKLLSPKQPLLRSQLKTLGRLLCFSHAFVGLSKVTTWYQYGFVQPQGPKANILVSGNEIRQFTRFMTERLNVSHAGAPLGEEYILVFSRTQNRLILNEAELLLELAQEFQMKTVTVSLEDHTFADVVRLVSNASMLVSMHGAQLVTALFLPRGATVVELFPYAVNPDHYTPYKTLATLPGMDLQYVAWRNMIRENTVTHPERPWDQGGITHLDRAEQARILQSREVPRHLCCRNPEWLFRIYQDTRVDIPSLMQSIRRVVKGRPGPRRQRWAISLYPGKVREARCQASVQGATEARLSVSWQIPWNLKYLKVREVRYEVWLQEQGENTYVPYMLTLQNHTFTENIKPFTTYLVWVRCIFNRSLLGPFADVLVCST

Target ORF information:

RefSeq Version	XM_008766683.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus protein O-linked mannose N-acetylglucosaminyltransferase 2 (beta 1,4-) (Pomgnt2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008766683.2

ORF Insert Sequence:

ATGCACCTCT CTGCAGTATT CAACGCCCTC CTGGTATCAG TGCTGGCTGC GGTCCTGTGG 
AAGCACGTGC GGTTGCGGGA GCATGCGGCC ACGCTAGAGG AGGAGCTGGC CCTTGGACAG 
CAGTCCCTGG ATCCAGTCCC AGGGCTGAGG ATCGACTACC CGAAGGCCCT GCAGATCCTC 
ATGGAGGGTG GCACCCACAT GGTGTGCACG GGCCGCACCC ACACGGACCG CATCTGCCGC 
TTCAAGTGGC TCTGCTACTC CAATGAGGCC GAAGAATTCA TCTTTTTCCA CGGCAACTCG 
TCTGTGATGC TGCCCAACCT GGGCTCCCGG CGCTTCCAGC CGGCCCTGCT GGACCTGTCC 
ACTGTGGAGG ACCATAATGC CCAGTACTTC AACTTCGTGG AGCTGCCTGC CGCAGCCCTG 
CGCTTCATGC CAAAGCCAGT GTTCGTGCCT GATGTGGCCC TCATCGCCAA CCGCTTCAAC 
CCCGATAACC TCATGCACGT CTTCCATGAT GACCTGCTCC CCCTCTTCTA CACACTGAGG 
CAGTTCCCCG GCCTGGCCCA AGAGGCCCGG CTCTTCTTTA TGGAGGGGTG GGGCGAGGGC 
GCCCACTTTG ACCTCTATAA ACTCCTCAGC CCCAAGCAGC CGCTGCTCCG TTCGCAGCTC 
AAGACCCTGG GCCGGCTGCT CTGCTTTTCC CATGCTTTCG TGGGCCTGTC CAAGGTCACC 
ACCTGGTACC AGTACGGCTT TGTCCAGCCC CAGGGCCCAA AGGCCAACAT CCTGGTCTCT 
GGCAATGAGA TCCGGCAGTT CACCCGCTTC ATGACTGAAA GGCTGAACGT GAGCCACGCG 
GGGGCGCCCC TCGGGGAGGA ATACATTCTG GTCTTCAGCC GCACCCAGAA TAGACTCATC 
CTGAATGAGG CAGAGCTGTT GCTGGAGCTA GCACAAGAGT TCCAGATGAA GACCGTGACA 
GTGTCCCTGG AGGACCACAC CTTTGCCGAT GTCGTGCGGC TGGTCAGCAA CGCCTCCATG 
TTAGTCAGCA TGCATGGGGC CCAGCTGGTG ACAGCCCTCT TCCTGCCCCG TGGGGCTACT 
GTGGTTGAAC TCTTCCCGTA CGCTGTCAAT CCTGACCACT ACACCCCCTA TAAGACGCTG 
GCCACACTGC CTGGCATGGA CCTGCAGTAT GTAGCCTGGA GAAACATGAT CCGGGAGAAC 
ACAGTCACGC ACCCCGAGCG ACCCTGGGAC CAAGGGGGAA TCACCCACTT GGACCGGGCT 
GAGCAGGCCC GCATCCTACA GAGCCGAGAG GTTCCCCGGC ATCTGTGTTG CCGGAACCCA 
GAGTGGCTAT TCCGAATCTA CCAGGACACC AGGGTGGACA TCCCATCCCT CATGCAATCC 
ATTCGGCGTG TGGTGAAGGG CCGGCCGGGG CCACGGAGGC AGAGGTGGGC AATCAGCCTG 
TACCCTGGTA AGGTACGGGA GGCTCGATGT CAGGCGTCGG TGCAGGGCGC CACGGAGGCC 
CGCCTGTCTG TGTCCTGGCA GATCCCGTGG AACCTCAAGT ACCTGAAAGT GAGGGAGGTG 
AGGTACGAGG TGTGGCTGCA GGAGCAAGGG GAGAACACGT ATGTGCCTTA CATGCTGACG 
CTGCAGAACC ATACCTTCAC GGAGAACATC AAACCTTTTA CCACCTACCT GGTGTGGGTC 
CGCTGCATCT TCAACAGGAG CCTCCTGGGA CCTTTTGCAG ATGTGCTGGT GTGCAGCACG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Milder forms of muscular dystrophy associated with POMGNT2 mutations.
Neurology. Genetics1(4)e33(2015 Dec)
Endo Y,Dong M,Noguchi S,Ogawa M,Hayashi YK,Kuru S,Sugiyama K,Nagai S,Ozasa S,Nonaka I,Nishino I

Ectopic clustering of Cajal-Retzius and subplate cells is an initial pathological feature in Pomgnt2-knockout mice, a model of dystroglycanopathy.
Scientific reports511163(2015 Jun)
Nakagawa N,Yagi H,Kato K,Takematsu H,Oka S

AGO61-dependent GlcNAc modification primes the formation of functional glycans on ?-dystroglycan.
Scientific reports33288(2013 Nov)
Yagi H,Nakagawa N,Saito T,Kiyonari H,Abe T,Toda T,Wu SW,Khoo KH,Oka S,Kato K

SGK196 is a glycosylation-specific O-mannose kinase required for dystroglycan function.
Science (New York, N.Y.)341(6148)896-9(2013 Aug)
Yoshida-Moriguchi T,Willer T,Anderson ME,Venzke D,Whyte T,Muntoni F,Lee H,Nelson SF,Yu L,Campbell KP

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Pomgnt2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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