Topbp1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Topbp1
Entrez Gene ID	315969
Full Name	DNA topoisomerase II binding protein 1
Synonyms	RGD1562949
General protein information	Preferred Names DNA topoisomerase II binding protein 1 Names DNA topoisomerase 2-binding protein 1 topoisomerase (DNA) II beta binding protein topoisomerase (DNA) II binding protein 1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 8 Map Location: 8q32

mRNA and Protein(s)

mRNA	Protein	Name
XM_236578.8	XP_236578.7	DNA topoisomerase 2-binding protein 1 isoform X2
XM_001072207.5	XP_001072207.4	DNA topoisomerase 2-binding protein 1 isoform X3
XM_008757856.2	XP_008756078.2	DNA topoisomerase 2-binding protein 1 isoform X1
XM_006243684.2	XP_006243746.2	DNA topoisomerase 2-binding protein 1 isoform X1
XM_008757855.2	XP_008756077.1	DNA topoisomerase 2-binding protein 1 isoform X3
XM_008766589.2	XP_008764811.1	DNA topoisomerase 2-binding protein 1 isoform X1
XM_006226542.2	XP_006226604.1	DNA topoisomerase 2-binding protein 1 isoform X4

Pathways from BioSystems

Homologies

Bos taurus (cattle)	TOPBP1	NP_001193695.1
Rattus norvegicus (Norway rat)	Topbp1	XP_236578.6
Homo sapiens (human)	TOPBP1	NP_008958.2
Danio rerio (zebrafish)	topbp1	NP_001186667.1
Xenopus tropicalis (tropical clawed frog)	topbp1	XP_004915426.1
	TOPBP1	XP_001112636.2
Mus musculus (house mouse)	Topbp1	NP_795953.2
Gallus gallus (chicken)	TOPBP1	XP_418794.1
Pan troglodytes (chimpanzee)	TOPBP1	XP_516761.3
Canis lupus familiaris (dog)	TOPBP1	XP_005634503.1

Gene Ontology

The following Topbp1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Topbp1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa47492	XM_008757856.2 Latest version!	Rattus norvegicus topoisomerase (DNA) II binding protein 1 (Topbp1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
ORa47492	XM_006243684.2 Latest version!	Rattus norvegicus topoisomerase (DNA) II binding protein 1 (Topbp1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
ORa47492	XM_008766589.2 Latest version!	Rattus norvegicus topoisomerase (DNA) II binding protein 1 (Topbp1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
ORa18094	XM_006226542.2 Latest version!	Rattus norvegicus topoisomerase (DNA) II binding protein 1 (Topbp1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
ORa47491	XM_236578.8 Latest version!	Rattus norvegicus topoisomerase (DNA) II binding protein 1 (Topbp1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
ORa47491	XM_001072207.5 Latest version!	Rattus norvegicus topoisomerase (DNA) II binding protein 1 (Topbp1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
ORa47491	XM_008757855.2 Latest version!	Rattus norvegicus topoisomerase (DNA) II binding protein 1 (Topbp1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Topbp1 ( XM_008757856.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008756078.2 Rattus norvegicus topoisomerase (DNA) II binding protein 1 (Topbp1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa47492
Clone ID Related Accession (Same CDS sequence)	XM_008757856.2 , XM_006243684.2 , XM_008766589.2
Accession Version	XM_008757856.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3987bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	DNA topoisomerase 2-binding protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (AC_000076.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008757856.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAGAA ATGACCAAGA GCCGTTTTTT GTGAAGTTTT TAAAGTCTTC GGACAATTCA 
GAATGTTTTT TTAAAGCTCT TGAGTCAATA AAGGAGTTAC AATCAGAAGA GTATCTTCAG 
GTTATTACCG ATGAGGAAGC ATTGAGGATT AAAGAGAATG ACAAATCGCT TTATATCTGT 
GACCGTTTCA GTGGCACTGC TTTTGATCAC CTTAAACAGC TTGGCTGCAG AATTGTTGGT 
CCTCAAGTAG TCACATTTTG TATGCGCCAC CAGCAGTGTG TCCCAAGAGC TGAACATCCA 
GTTTATAATA TGATTATGTC TGACATAACT ATATCTTGTA CAAGCCTGGA TAAAGACAAG 
AGGGAAGAAG TTCATAAATA CGTACAAATG ATGGGTGGAC GAGTATACAG AGACCTTAAT 
GTCTCAGTCA CTCACCTTAT TGCAGGAGAA GTTGGGAGCA AAAAATACTT AGTTGCTGCT 
AATCTGAAGA AACCTATTTT GCTTCCTGCT TGGATTAAAA CACTTTGGGA GAAGTCACAA 
GAAAAAAAAA TAACCAAATA CACTGATGTA AACATGGAAG ACTTTAAGTG TCCTATTTTC 
CTTGGTTGCA TTATCTGTGT GACTGGCTTA AATGGTATAC ACAGGAAGAC AGTCCAGCAG 
CTCACAGCTA AGCATGGAGG CCAGTACATG GGGCAGCTGA AGATGAACGA GTGCACACAC 
CTCATTGTGC AAGAACCAAA AGGACAAAAA TATGAATGTG CCAGGAGATG GAATGTGCAC 
TGTGTGACCC TGCAATGGTT CCATGACAGT ATTGAGAAAG GCTTTTGTCA GGATGAGTCT 
ATATACAAGG CCGAACCCAG AGCAGCGGTA AAGACGGTGC CTGATACTTC CACACCCACT 
GCTCAGAGCG GCAGTGCTGC AGCTGAGAAT CACAGGCTGG CAGATGTCAG CCATATATCC 
AACATCAACG GCAGCTGTGT AAATGAAACA ATGTTTGGTT CAACTACGAG CAAACTCGAA 
TGCACACTTG AGAACCTAGA AAATCTGGAC ATCAGTATGT TTCAAGCACC TGAAGATCTA 
TTAGATGGCT GTCGGATATA TCTTTGTGGC TTTAGTGGCA GAAAGCTCGA TAAGTTGAGA 
AGGCTTATTA ACAGTGGAGG TGGAGTTCGG TTTAATCAAC TCAATGAAGA TGTAACTCAT 
GTAATCATGG GAGATTATGA GGATGACGTG AGGCAGTTTT GGAGCAAATC CTCCCACAGG 
CCCCATGTAG TGGGAGCAAA GTGGTTGCTG GAGTGTTTTA GTAAAGGATA TATACTTCCT 
GAAGAGTCAT ATATCCACGC TAACTACCAG CCAGATGGAA TTCCAGTTTC AGATCAGCCC 
AGAAACCAGA CAACTGTCTT AGAAAAGAGT GGCACATTCT CTAAGAAGGA CTTTGTTCCT 
AATGAGAAGT TTCAGCAGAC TGATGAGGAT CTGCTTGCTC AGTATGTACA TGATGACTCC 
ACAATAGTTG AAGCTAAGCT ATCTGAAGCT ATAGAGCCTG AAGTTGGGCC TTGCGGTGGT 
TCTGCTCATG TTGAGCCCTG TGATGATTCC ACTCACATTT CTGTGCAAGA GGAAAACAAG 
TCGTCTGTCA GTCATTGCCT CCTTGATGGC TCTACAGTTC CTGAGGAAGG CTTATTTAGC 
CAAAAGAGTT TCCTTGTTTT GGGTTTTAGT GTTGAAAATG AATGTAATAT TGTAAACATC 
ATAAGAGAGC ATGCTGGAAA GATTGTGTCC CTTCCCAGCA GAATTGTTGC TGACTATGCC 
GTGGTTCCCC TGCTGGGATG TGAAGTAGAA GCGACAGTGG CAGAAGTTGT TACAAACACA 
TGGCTGGTTA CATGCATAGA CAACCAGACA TTAGTTGATC CCAAGTCGAA TCCCCTCTTC 
ACGCCAGTCT CAGTAATGTC AGGAATGACT CCTTTAGAGG ACTGTGTGAT TTCCTTCAGT 
CAGTGTGTGG GAGCAGAAAG AGATTCCTTG GTATTCTTAG CAAATCATCT TGGAGCAAGT 
GTTCAAGAAT TCTTTGTCCG CAAATCCAAT GCAAAGAAAG GCATGCTCGC CAGTACACAT 
CTTATAGTGA AAGAACCGAC TGGTTCTAAA TATGAAGCTG CAAAGAAATG GAGTTTGCCA 
GCAGTTAATA TTTCGTGGCT CTTAGAAACT GCTAGAATGG GGAAGAGAGC AGATGAAAAC 
CATTTTCTGG TTGACAGTGC ACCTAAACAA GAACAAGTAT TAGAAACTAA CATACCAAAT 
GGAGTGAGTC CAAATCCAGA TTCTCCAGCA CATCCTGATG CACACTTGGA AATTCACAGA 
CAAAAGGCCG TTACACCTTT GGACATGAAC CGATTTCAGA GTAAAGCTTT CCATGCTGTC 
ATCTCACAGC ACAACAGGCA GGGCTTGGCC TCCCCACCAG TGAGACAGCC ACCTAAGAAG 
GAACCCTCCT TCCACCTGGA CACACCCTCA AAGTTCCTGT CCAAGGACAA GCTTTTCAAG 
CCATCCTTTG ATGTGACGGA TGCACTTGCA GCCTTAGAAA CCCCAAATGC TGCCAGCCAG 
AAGCGGAAAC TGAGCTCACC ACTCTCTGAA GTCATCGTCA GAAACCTGAC AACTGCTTTG 
GCAAATAGTT CACGAAACAC CGGCTGTCTT TCTGCTAGCC CTCAGCTAAA GGGTGCCCAT 
TTGGAGAAGG AAGAGACTCC GAAACCGCTT GACAAAGTTG TGGTGTGCGT GAGTAAAAGG 
CTCAGTAAGA AGCAGAGTGA GCTAAATGGG GTTGCAGCCT CACTGGGAGC AGAGTACAGG 
TGGAGTTTTG ATGAGACAGT TACTCATTAC ATTTACCAGG GTCGGGCAAA TGATAGTAAT 
CGCGAGTATA AATCTGCAAA GGAAAGAGGG GTGCACGTCG TGTCGGAGCA TTGGCTGTTA 
GAGTGTGCTC AAGAGTATAA GCATCTTCCA GAATCTCTTT ACCCACACAC TTACAACCCC 
AAAATGAGCT TGGACATCAG CACAGTGCAG GACGGGCGGC TCTGTGACAG CCGCTTACCC 
TCGGCACTTT CTGCATCTAA GGACGATGAG CCAGATCACC TGTCTGTAGA AGAAAATGCA 
ACAGAGACTT TGGGCACACA CAGCAGAGAG TCCGCTCTGC TGAATGGGAA TGGAAGGGAC 
GACTGCAAAG GAGCTCTGAC CCAGGCCTTG GAGATGAGAG AGAACTTCCA GAAACAACTA 
CAAGAGATCA TGTCAGCAAC CTCCATCGTG AGGACACCAG CCCAGAAGAC TTGCATGTCA 
AGAAGTAGTT GTAACAGCGC CTCTTCAACT CCTGACAGCA CTCGCTCTGC TCGGAGTGGA 
CGAAGCCGAG TCTTGGAGGC GCTCAGGCAG TCTCGACAGA TAGTGCCTGA TATCAACACA 
GAGCCCTCCC AGAATGAACA GATCATTTGG GATGACCCAA CAGCAAGGGA GGAGAGAGCA 
AGGCTTGCCA GCAACCTACA GTGGCCTAGC TACCCAACAC AGTATTCCGA GCTTCAGGTT 
GAGATAAAAA AACTGGATGA TTCTCCTTCT CGAAAGCCTG TGTACCATTC AGAAATTGCT 
GGGCAGGCCT CGTGTGTGAC TCAAGCCCCA GGGCAGCACC CAGGCTCTGA AGAACCAGAA 
GCTCCAGCAG CAGAAAGGAA TCTGATCCCT GAGCCTCAAG CCCCCAGCGT GGCTTTCCCC 
CTGGCTAAGC CGCCTGTAGC TCCACAACCT GAGGACAAGA TTGAAACACT GGAGGAGACG 
CATGGAAAAC TTAAAAAACA GTATGTGTTC CAGATGTCAT CTCTGAATTC TCAAGAGCGG 
ATTGATTACT GTCGCCTGAT TAAAGACCTA GGCCCGGGAG TGCCCAGTGT GATAGTGCAC 
ACATGGAGCG CAGAACAGGT TCCGGGGAGG ACTCCCACCT GTCTTGGCCA AAATAAAAAG 
ATCCATGGAC TCTGCAGTCA GAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008756078.2
CDS	206..4192
Translation	MSRNDQEPFFVKFLKSSDNSECFFKALESIKELQSEEYLQVITDEEALRIKENDKSLYICDRFSGTAFDHLKQLGCRIVGPQVVTFCMRHQQCVPRAEHPVYNMIMSDITISCTSLDKDKREEVHKYVQMMGGRVYRDLNVSVTHLIAGEVGSKKYLVAANLKKPILLPAWIKTLWEKSQEKKITKYTDVNMEDFKCPIFLGCIICVTGLNGIHRKTVQQLTAKHGGQYMGQLKMNECTHLIVQEPKGQKYECARRWNVHCVTLQWFHDSIEKGFCQDESIYKAEPRAAVKTVPDTSTPTAQSGSAAAENHRLADVSHISNINGSCVNETMFGSTTSKLECTLENLENLDISMFQAPEDLLDGCRIYLCGFSGRKLDKLRRLINSGGGVRFNQLNEDVTHVIMGDYEDDVRQFWSKSSHRPHVVGAKWLLECFSKGYILPEESYIHANYQPDGIPVSDQPRNQTTVLEKSGTFSKKDFVPNEKFQQTDEDLLAQYVHDDSTIVEAKLSEAIEPEVGPCGGSAHVEPCDDSTHISVQEENKSSVSHCLLDGSTVPEEGLFSQKSFLVLGFSVENECNIVNIIREHAGKIVSLPSRIVADYAVVPLLGCEVEATVAEVVTNTWLVTCIDNQTLVDPKSNPLFTPVSVMSGMTPLEDCVISFSQCVGAERDSLVFLANHLGASVQEFFVRKSNAKKGMLASTHLIVKEPTGSKYEAAKKWSLPAVNISWLLETARMGKRADENHFLVDSAPKQEQVLETNIPNGVSPNPDSPAHPDAHLEIHRQKAVTPLDMNRFQSKAFHAVISQHNRQGLASPPVRQPPKKEPSFHLDTPSKFLSKDKLFKPSFDVTDALAALETPNAASQKRKLSSPLSEVIVRNLTTALANSSRNTGCLSASPQLKGAHLEKEETPKPLDKVVVCVSKRLSKKQSELNGVAASLGAEYRWSFDETVTHYIYQGRANDSNREYKSAKERGVHVVSEHWLLECAQEYKHLPESLYPHTYNPKMSLDISTVQDGRLCDSRLPSALSASKDDEPDHLSVEENATETLGTHSRESALLNGNGRDDCKGALTQALEMRENFQKQLQEIMSATSIVRTPAQKTCMSRSSCNSASSTPDSTRSARSGRSRVLEALRQSRQIVPDINTEPSQNEQIIWDDPTAREERARLASNLQWPSYPTQYSELQVEIKKLDDSPSRKPVYHSEIAGQASCVTQAPGQHPGSEEPEAPAAERNLIPEPQAPSVAFPLAKPPVAPQPEDKIETLEETHGKLKKQYVFQMSSLNSQERIDYCRLIKDLGPGVPSVIVHTWSAEQVPGRTPTCLGQNKKIHGLCSQK

Target ORF information:

RefSeq Version	XM_008757856.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus topoisomerase (DNA) II binding protein 1 (Topbp1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008757856.2

ORF Insert Sequence:

ATGTCCAGAA ATGACCAAGA GCCGTTTTTT GTGAAGTTTT TAAAGTCTTC GGACAATTCA 
GAATGTTTTT TTAAAGCTCT TGAGTCAATA AAGGAGTTAC AATCAGAAGA GTATCTTCAG 
GTTATTACCG ATGAGGAAGC ATTGAGGATT AAAGAGAATG ACAAATCGCT TTATATCTGT 
GACCGTTTCA GTGGCACTGC TTTTGATCAC CTTAAACAGC TTGGCTGCAG AATTGTTGGT 
CCTCAAGTAG TCACATTTTG TATGCGCCAC CAGCAGTGTG TCCCAAGAGC TGAACATCCA 
GTTTATAATA TGATTATGTC TGACATAACT ATATCTTGTA CAAGCCTGGA TAAAGACAAG 
AGGGAAGAAG TTCATAAATA CGTACAAATG ATGGGTGGAC GAGTATACAG AGACCTTAAT 
GTCTCAGTCA CTCACCTTAT TGCAGGAGAA GTTGGGAGCA AAAAATACTT AGTTGCTGCT 
AATCTGAAGA AACCTATTTT GCTTCCTGCT TGGATTAAAA CACTTTGGGA GAAGTCACAA 
GAAAAAAAAA TAACCAAATA CACTGATGTA AACATGGAAG ACTTTAAGTG TCCTATTTTC 
CTTGGTTGCA TTATCTGTGT GACTGGCTTA AATGGTATAC ACAGGAAGAC AGTCCAGCAG 
CTCACAGCTA AGCATGGAGG CCAGTACATG GGGCAGCTGA AGATGAACGA GTGCACACAC 
CTCATTGTGC AAGAACCAAA AGGACAAAAA TATGAATGTG CCAGGAGATG GAATGTGCAC 
TGTGTGACCC TGCAATGGTT CCATGACAGT ATTGAGAAAG GCTTTTGTCA GGATGAGTCT 
ATATACAAGG CCGAACCCAG AGCAGCGGTA AAGACGGTGC CTGATACTTC CACACCCACT 
GCTCAGAGCG GCAGTGCTGC AGCTGAGAAT CACAGGCTGG CAGATGTCAG CCATATATCC 
AACATCAACG GCAGCTGTGT AAATGAAACA ATGTTTGGTT CAACTACGAG CAAACTCGAA 
TGCACACTTG AGAACCTAGA AAATCTGGAC ATCAGTATGT TTCAAGCACC TGAAGATCTA 
TTAGATGGCT GTCGGATATA TCTTTGTGGC TTTAGTGGCA GAAAGCTCGA TAAGTTGAGA 
AGGCTTATTA ACAGTGGAGG TGGAGTTCGG TTTAATCAAC TCAATGAAGA TGTAACTCAT 
GTAATCATGG GAGATTATGA GGATGACGTG AGGCAGTTTT GGAGCAAATC CTCCCACAGG 
CCCCATGTAG TGGGAGCAAA GTGGTTGCTG GAGTGTTTTA GTAAAGGATA TATACTTCCT 
GAAGAGTCAT ATATCCACGC TAACTACCAG CCAGATGGAA TTCCAGTTTC AGATCAGCCC 
AGAAACCAGA CAACTGTCTT AGAAAAGAGT GGCACATTCT CTAAGAAGGA CTTTGTTCCT 
AATGAGAAGT TTCAGCAGAC TGATGAGGAT CTGCTTGCTC AGTATGTACA TGATGACTCC 
ACAATAGTTG AAGCTAAGCT ATCTGAAGCT ATAGAGCCTG AAGTTGGGCC TTGCGGTGGT 
TCTGCTCATG TTGAGCCCTG TGATGATTCC ACTCACATTT CTGTGCAAGA GGAAAACAAG 
TCGTCTGTCA GTCATTGCCT CCTTGATGGC TCTACAGTTC CTGAGGAAGG CTTATTTAGC 
CAAAAGAGTT TCCTTGTTTT GGGTTTTAGT GTTGAAAATG AATGTAATAT TGTAAACATC 
ATAAGAGAGC ATGCTGGAAA GATTGTGTCC CTTCCCAGCA GAATTGTTGC TGACTATGCC 
GTGGTTCCCC TGCTGGGATG TGAAGTAGAA GCGACAGTGG CAGAAGTTGT TACAAACACA 
TGGCTGGTTA CATGCATAGA CAACCAGACA TTAGTTGATC CCAAGTCGAA TCCCCTCTTC 
ACGCCAGTCT CAGTAATGTC AGGAATGACT CCTTTAGAGG ACTGTGTGAT TTCCTTCAGT 
CAGTGTGTGG GAGCAGAAAG AGATTCCTTG GTATTCTTAG CAAATCATCT TGGAGCAAGT 
GTTCAAGAAT TCTTTGTCCG CAAATCCAAT GCAAAGAAAG GCATGCTCGC CAGTACACAT 
CTTATAGTGA AAGAACCGAC TGGTTCTAAA TATGAAGCTG CAAAGAAATG GAGTTTGCCA 
GCAGTTAATA TTTCGTGGCT CTTAGAAACT GCTAGAATGG GGAAGAGAGC AGATGAAAAC 
CATTTTCTGG TTGACAGTGC ACCTAAACAA GAACAAGTAT TAGAAACTAA CATACCAAAT 
GGAGTGAGTC CAAATCCAGA TTCTCCAGCA CATCCTGATG CACACTTGGA AATTCACAGA 
CAAAAGGCCG TTACACCTTT GGACATGAAC CGATTTCAGA GTAAAGCTTT CCATGCTGTC 
ATCTCACAGC ACAACAGGCA GGGCTTGGCC TCCCCACCAG TGAGACAGCC ACCTAAGAAG 
GAACCCTCCT TCCACCTGGA CACACCCTCA AAGTTCCTGT CCAAGGACAA GCTTTTCAAG 
CCATCCTTTG ATGTGACGGA TGCACTTGCA GCCTTAGAAA CCCCAAATGC TGCCAGCCAG 
AAGCGGAAAC TGAGCTCACC ACTCTCTGAA GTCATCGTCA GAAACCTGAC AACTGCTTTG 
GCAAATAGTT CACGAAACAC CGGCTGTCTT TCTGCTAGCC CTCAGCTAAA GGGTGCCCAT 
TTGGAGAAGG AAGAGACTCC GAAACCGCTT GACAAAGTTG TGGTGTGCGT GAGTAAAAGG 
CTCAGTAAGA AGCAGAGTGA GCTAAATGGG GTTGCAGCCT CACTGGGAGC AGAGTACAGG 
TGGAGTTTTG ATGAGACAGT TACTCATTAC ATTTACCAGG GTCGGGCAAA TGATAGTAAT 
CGCGAGTATA AATCTGCAAA GGAAAGAGGG GTGCACGTCG TGTCGGAGCA TTGGCTGTTA 
GAGTGTGCTC AAGAGTATAA GCATCTTCCA GAATCTCTTT ACCCACACAC TTACAACCCC 
AAAATGAGCT TGGACATCAG CACAGTGCAG GACGGGCGGC TCTGTGACAG CCGCTTACCC 
TCGGCACTTT CTGCATCTAA GGACGATGAG CCAGATCACC TGTCTGTAGA AGAAAATGCA 
ACAGAGACTT TGGGCACACA CAGCAGAGAG TCCGCTCTGC TGAATGGGAA TGGAAGGGAC 
GACTGCAAAG GAGCTCTGAC CCAGGCCTTG GAGATGAGAG AGAACTTCCA GAAACAACTA 
CAAGAGATCA TGTCAGCAAC CTCCATCGTG AGGACACCAG CCCAGAAGAC TTGCATGTCA 
AGAAGTAGTT GTAACAGCGC CTCTTCAACT CCTGACAGCA CTCGCTCTGC TCGGAGTGGA 
CGAAGCCGAG TCTTGGAGGC GCTCAGGCAG TCTCGACAGA TAGTGCCTGA TATCAACACA 
GAGCCCTCCC AGAATGAACA GATCATTTGG GATGACCCAA CAGCAAGGGA GGAGAGAGCA 
AGGCTTGCCA GCAACCTACA GTGGCCTAGC TACCCAACAC AGTATTCCGA GCTTCAGGTT 
GAGATAAAAA AACTGGATGA TTCTCCTTCT CGAAAGCCTG TGTACCATTC AGAAATTGCT 
GGGCAGGCCT CGTGTGTGAC TCAAGCCCCA GGGCAGCACC CAGGCTCTGA AGAACCAGAA 
GCTCCAGCAG CAGAAAGGAA TCTGATCCCT GAGCCTCAAG CCCCCAGCGT GGCTTTCCCC 
CTGGCTAAGC CGCCTGTAGC TCCACAACCT GAGGACAAGA TTGAAACACT GGAGGAGACG 
CATGGAAAAC TTAAAAAACA GTATGTGTTC CAGATGTCAT CTCTGAATTC TCAAGAGCGG 
ATTGATTACT GTCGCCTGAT TAAAGACCTA GGCCCGGGAG TGCCCAGTGT GATAGTGCAC 
ACATGGAGCG CAGAACAGGT TCCGGGGAGG ACTCCCACCT GTCTTGGCCA AAATAAAAAG 
ATCCATGGAC TCTGCAGTCA GAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Topbp1 ( XM_006243684.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006243746.2 Rattus norvegicus topoisomerase (DNA) II binding protein 1 (Topbp1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa47492
Clone ID Related Accession (Same CDS sequence)	XM_008757856.2 , XM_006243684.2 , XM_008766589.2
Accession Version	XM_006243684.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3987bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	DNA topoisomerase 2-binding protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005107.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006243684.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAGAA ATGACCAAGA GCCGTTTTTT GTGAAGTTTT TAAAGTCTTC GGACAATTCA 
GAATGTTTTT TTAAAGCTCT TGAGTCAATA AAGGAGTTAC AATCAGAAGA GTATCTTCAG 
GTTATTACCG ATGAGGAAGC ATTGAGGATT AAAGAGAATG ACAAATCGCT TTATATCTGT 
GACCGTTTCA GTGGCACTGC TTTTGATCAC CTTAAACAGC TTGGCTGCAG AATTGTTGGT 
CCTCAAGTAG TCACATTTTG TATGCGCCAC CAGCAGTGTG TCCCAAGAGC TGAACATCCA 
GTTTATAATA TGATTATGTC TGACATAACT ATATCTTGTA CAAGCCTGGA TAAAGACAAG 
AGGGAAGAAG TTCATAAATA CGTACAAATG ATGGGTGGAC GAGTATACAG AGACCTTAAT 
GTCTCAGTCA CTCACCTTAT TGCAGGAGAA GTTGGGAGCA AAAAATACTT AGTTGCTGCT 
AATCTGAAGA AACCTATTTT GCTTCCTGCT TGGATTAAAA CACTTTGGGA GAAGTCACAA 
GAAAAAAAAA TAACCAAATA CACTGATGTA AACATGGAAG ACTTTAAGTG TCCTATTTTC 
CTTGGTTGCA TTATCTGTGT GACTGGCTTA AATGGTATAC ACAGGAAGAC AGTCCAGCAG 
CTCACAGCTA AGCATGGAGG CCAGTACATG GGGCAGCTGA AGATGAACGA GTGCACACAC 
CTCATTGTGC AAGAACCAAA AGGACAAAAA TATGAATGTG CCAGGAGATG GAATGTGCAC 
TGTGTGACCC TGCAATGGTT CCATGACAGT ATTGAGAAAG GCTTTTGTCA GGATGAGTCT 
ATATACAAGG CCGAACCCAG AGCAGCGGTA AAGACGGTGC CTGATACTTC CACACCCACT 
GCTCAGAGCG GCAGTGCTGC AGCTGAGAAT CACAGGCTGG CAGATGTCAG CCATATATCC 
AACATCAACG GCAGCTGTGT AAATGAAACA ATGTTTGGTT CAACTACGAG CAAACTCGAA 
TGCACACTTG AGAACCTAGA AAATCTGGAC ATCAGTATGT TTCAAGCACC TGAAGATCTA 
TTAGATGGCT GTCGGATATA TCTTTGTGGC TTTAGTGGCA GAAAGCTCGA TAAGTTGAGA 
AGGCTTATTA ACAGTGGAGG TGGAGTTCGG TTTAATCAAC TCAATGAAGA TGTAACTCAT 
GTAATCATGG GAGATTATGA GGATGACGTG AGGCAGTTTT GGAGCAAATC CTCCCACAGG 
CCCCATGTAG TGGGAGCAAA GTGGTTGCTG GAGTGTTTTA GTAAAGGATA TATACTTCCT 
GAAGAGTCAT ATATCCACGC TAACTACCAG CCAGATGGAA TTCCAGTTTC AGATCAGCCC 
AGAAACCAGA CAACTGTCTT AGAAAAGAGT GGCACATTCT CTAAGAAGGA CTTTGTTCCT 
AATGAGAAGT TTCAGCAGAC TGATGAGGAT CTGCTTGCTC AGTATGTACA TGATGACTCC 
ACAATAGTTG AAGCTAAGCT ATCTGAAGCT ATAGAGCCTG AAGTTGGGCC TTGCGGTGGT 
TCTGCTCATG TTGAGCCCTG TGATGATTCC ACTCACATTT CTGTGCAAGA GGAAAACAAG 
TCGTCTGTCA GTCATTGCCT CCTTGATGGC TCTACAGTTC CTGAGGAAGG CTTATTTAGC 
CAAAAGAGTT TCCTTGTTTT GGGTTTTAGT GTTGAAAATG AATGTAATAT TGTAAACATC 
ATAAGAGAGC ATGCTGGAAA GATTGTGTCC CTTCCCAGCA GAATTGTTGC TGACTATGCC 
GTGGTTCCCC TGCTGGGATG TGAAGTAGAA GCGACAGTGG CAGAAGTTGT TACAAACACA 
TGGCTGGTTA CATGCATAGA CAACCAGACA TTAGTTGATC CCAAGTCGAA TCCCCTCTTC 
ACGCCAGTCT CAGTAATGTC AGGAATGACT CCTTTAGAGG ACTGTGTGAT TTCCTTCAGT 
CAGTGTGTGG GAGCAGAAAG AGATTCCTTG GTATTCTTAG CAAATCATCT TGGAGCAAGT 
GTTCAAGAAT TCTTTGTCCG CAAATCCAAT GCAAAGAAAG GCATGCTCGC CAGTACACAT 
CTTATAGTGA AAGAACCGAC TGGTTCTAAA TATGAAGCTG CAAAGAAATG GAGTTTGCCA 
GCAGTTAATA TTTCGTGGCT CTTAGAAACT GCTAGAATGG GGAAGAGAGC AGATGAAAAC 
CATTTTCTGG TTGACAGTGC ACCTAAACAA GAACAAGTAT TAGAAACTAA CATACCAAAT 
GGAGTGAGTC CAAATCCAGA TTCTCCAGCA CATCCTGATG CACACTTGGA AATTCACAGA 
CAAAAGGCCG TTACACCTTT GGACATGAAC CGATTTCAGA GTAAAGCTTT CCATGCTGTC 
ATCTCACAGC ACAACAGGCA GGGCTTGGCC TCCCCACCAG TGAGACAGCC ACCTAAGAAG 
GAACCCTCCT TCCACCTGGA CACACCCTCA AAGTTCCTGT CCAAGGACAA GCTTTTCAAG 
CCATCCTTTG ATGTGACGGA TGCACTTGCA GCCTTAGAAA CCCCAAATGC TGCCAGCCAG 
AAGCGGAAAC TGAGCTCACC ACTCTCTGAA GTCATCGTCA GAAACCTGAC AACTGCTTTG 
GCAAATAGTT CACGAAACAC CGGCTGTCTT TCTGCTAGCC CTCAGCTAAA GGGTGCCCAT 
TTGGAGAAGG AAGAGACTCC GAAACCGCTT GACAAAGTTG TGGTGTGCGT GAGTAAAAGG 
CTCAGTAAGA AGCAGAGTGA GCTAAATGGG GTTGCAGCCT CACTGGGAGC AGAGTACAGG 
TGGAGTTTTG ATGAGACAGT TACTCATTAC ATTTACCAGG GTCGGGCAAA TGATAGTAAT 
CGCGAGTATA AATCTGCAAA GGAAAGAGGG GTGCACGTCG TGTCGGAGCA TTGGCTGTTA 
GAGTGTGCTC AAGAGTATAA GCATCTTCCA GAATCTCTTT ACCCACACAC TTACAACCCC 
AAAATGAGCT TGGACATCAG CACAGTGCAG GACGGGCGGC TCTGTGACAG CCGCTTACCC 
TCGGCACTTT CTGCATCTAA GGACGATGAG CCAGATCACC TGTCTGTAGA AGAAAATGCA 
ACAGAGACTT TGGGCACACA CAGCAGAGAG TCCGCTCTGC TGAATGGGAA TGGAAGGGAC 
GACTGCAAAG GAGCTCTGAC CCAGGCCTTG GAGATGAGAG AGAACTTCCA GAAACAACTA 
CAAGAGATCA TGTCAGCAAC CTCCATCGTG AGGACACCAG CCCAGAAGAC TTGCATGTCA 
AGAAGTAGTT GTAACAGCGC CTCTTCAACT CCTGACAGCA CTCGCTCTGC TCGGAGTGGA 
CGAAGCCGAG TCTTGGAGGC GCTCAGGCAG TCTCGACAGA TAGTGCCTGA TATCAACACA 
GAGCCCTCCC AGAATGAACA GATCATTTGG GATGACCCAA CAGCAAGGGA GGAGAGAGCA 
AGGCTTGCCA GCAACCTACA GTGGCCTAGC TACCCAACAC AGTATTCCGA GCTTCAGGTT 
GAGATAAAAA AACTGGATGA TTCTCCTTCT CGAAAGCCTG TGTACCATTC AGAAATTGCT 
GGGCAGGCCT CGTGTGTGAC TCAAGCCCCA GGGCAGCACC CAGGCTCTGA AGAACCAGAA 
GCTCCAGCAG CAGAAAGGAA TCTGATCCCT GAGCCTCAAG CCCCCAGCGT GGCTTTCCCC 
CTGGCTAAGC CGCCTGTAGC TCCACAACCT GAGGACAAGA TTGAAACACT GGAGGAGACG 
CATGGAAAAC TTAAAAAACA GTATGTGTTC CAGATGTCAT CTCTGAATTC TCAAGAGCGG 
ATTGATTACT GTCGCCTGAT TAAAGACCTA GGCCCGGGAG TGCCCAGTGT GATAGTGCAC 
ACATGGAGCG CAGAACAGGT TCCGGGGAGG ACTCCCACCT GTCTTGGCCA AAATAAAAAG 
ATCCATGGAC TCTGCAGTCA GAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006243746.2
CDS	206..4192
Translation	MSRNDQEPFFVKFLKSSDNSECFFKALESIKELQSEEYLQVITDEEALRIKENDKSLYICDRFSGTAFDHLKQLGCRIVGPQVVTFCMRHQQCVPRAEHPVYNMIMSDITISCTSLDKDKREEVHKYVQMMGGRVYRDLNVSVTHLIAGEVGSKKYLVAANLKKPILLPAWIKTLWEKSQEKKITKYTDVNMEDFKCPIFLGCIICVTGLNGIHRKTVQQLTAKHGGQYMGQLKMNECTHLIVQEPKGQKYECARRWNVHCVTLQWFHDSIEKGFCQDESIYKAEPRAAVKTVPDTSTPTAQSGSAAAENHRLADVSHISNINGSCVNETMFGSTTSKLECTLENLENLDISMFQAPEDLLDGCRIYLCGFSGRKLDKLRRLINSGGGVRFNQLNEDVTHVIMGDYEDDVRQFWSKSSHRPHVVGAKWLLECFSKGYILPEESYIHANYQPDGIPVSDQPRNQTTVLEKSGTFSKKDFVPNEKFQQTDEDLLAQYVHDDSTIVEAKLSEAIEPEVGPCGGSAHVEPCDDSTHISVQEENKSSVSHCLLDGSTVPEEGLFSQKSFLVLGFSVENECNIVNIIREHAGKIVSLPSRIVADYAVVPLLGCEVEATVAEVVTNTWLVTCIDNQTLVDPKSNPLFTPVSVMSGMTPLEDCVISFSQCVGAERDSLVFLANHLGASVQEFFVRKSNAKKGMLASTHLIVKEPTGSKYEAAKKWSLPAVNISWLLETARMGKRADENHFLVDSAPKQEQVLETNIPNGVSPNPDSPAHPDAHLEIHRQKAVTPLDMNRFQSKAFHAVISQHNRQGLASPPVRQPPKKEPSFHLDTPSKFLSKDKLFKPSFDVTDALAALETPNAASQKRKLSSPLSEVIVRNLTTALANSSRNTGCLSASPQLKGAHLEKEETPKPLDKVVVCVSKRLSKKQSELNGVAASLGAEYRWSFDETVTHYIYQGRANDSNREYKSAKERGVHVVSEHWLLECAQEYKHLPESLYPHTYNPKMSLDISTVQDGRLCDSRLPSALSASKDDEPDHLSVEENATETLGTHSRESALLNGNGRDDCKGALTQALEMRENFQKQLQEIMSATSIVRTPAQKTCMSRSSCNSASSTPDSTRSARSGRSRVLEALRQSRQIVPDINTEPSQNEQIIWDDPTAREERARLASNLQWPSYPTQYSELQVEIKKLDDSPSRKPVYHSEIAGQASCVTQAPGQHPGSEEPEAPAAERNLIPEPQAPSVAFPLAKPPVAPQPEDKIETLEETHGKLKKQYVFQMSSLNSQERIDYCRLIKDLGPGVPSVIVHTWSAEQVPGRTPTCLGQNKKIHGLCSQK

Target ORF information:

RefSeq Version	XM_006243684.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus topoisomerase (DNA) II binding protein 1 (Topbp1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006243684.2

ORF Insert Sequence:

ATGTCCAGAA ATGACCAAGA GCCGTTTTTT GTGAAGTTTT TAAAGTCTTC GGACAATTCA 
GAATGTTTTT TTAAAGCTCT TGAGTCAATA AAGGAGTTAC AATCAGAAGA GTATCTTCAG 
GTTATTACCG ATGAGGAAGC ATTGAGGATT AAAGAGAATG ACAAATCGCT TTATATCTGT 
GACCGTTTCA GTGGCACTGC TTTTGATCAC CTTAAACAGC TTGGCTGCAG AATTGTTGGT 
CCTCAAGTAG TCACATTTTG TATGCGCCAC CAGCAGTGTG TCCCAAGAGC TGAACATCCA 
GTTTATAATA TGATTATGTC TGACATAACT ATATCTTGTA CAAGCCTGGA TAAAGACAAG 
AGGGAAGAAG TTCATAAATA CGTACAAATG ATGGGTGGAC GAGTATACAG AGACCTTAAT 
GTCTCAGTCA CTCACCTTAT TGCAGGAGAA GTTGGGAGCA AAAAATACTT AGTTGCTGCT 
AATCTGAAGA AACCTATTTT GCTTCCTGCT TGGATTAAAA CACTTTGGGA GAAGTCACAA 
GAAAAAAAAA TAACCAAATA CACTGATGTA AACATGGAAG ACTTTAAGTG TCCTATTTTC 
CTTGGTTGCA TTATCTGTGT GACTGGCTTA AATGGTATAC ACAGGAAGAC AGTCCAGCAG 
CTCACAGCTA AGCATGGAGG CCAGTACATG GGGCAGCTGA AGATGAACGA GTGCACACAC 
CTCATTGTGC AAGAACCAAA AGGACAAAAA TATGAATGTG CCAGGAGATG GAATGTGCAC 
TGTGTGACCC TGCAATGGTT CCATGACAGT ATTGAGAAAG GCTTTTGTCA GGATGAGTCT 
ATATACAAGG CCGAACCCAG AGCAGCGGTA AAGACGGTGC CTGATACTTC CACACCCACT 
GCTCAGAGCG GCAGTGCTGC AGCTGAGAAT CACAGGCTGG CAGATGTCAG CCATATATCC 
AACATCAACG GCAGCTGTGT AAATGAAACA ATGTTTGGTT CAACTACGAG CAAACTCGAA 
TGCACACTTG AGAACCTAGA AAATCTGGAC ATCAGTATGT TTCAAGCACC TGAAGATCTA 
TTAGATGGCT GTCGGATATA TCTTTGTGGC TTTAGTGGCA GAAAGCTCGA TAAGTTGAGA 
AGGCTTATTA ACAGTGGAGG TGGAGTTCGG TTTAATCAAC TCAATGAAGA TGTAACTCAT 
GTAATCATGG GAGATTATGA GGATGACGTG AGGCAGTTTT GGAGCAAATC CTCCCACAGG 
CCCCATGTAG TGGGAGCAAA GTGGTTGCTG GAGTGTTTTA GTAAAGGATA TATACTTCCT 
GAAGAGTCAT ATATCCACGC TAACTACCAG CCAGATGGAA TTCCAGTTTC AGATCAGCCC 
AGAAACCAGA CAACTGTCTT AGAAAAGAGT GGCACATTCT CTAAGAAGGA CTTTGTTCCT 
AATGAGAAGT TTCAGCAGAC TGATGAGGAT CTGCTTGCTC AGTATGTACA TGATGACTCC 
ACAATAGTTG AAGCTAAGCT ATCTGAAGCT ATAGAGCCTG AAGTTGGGCC TTGCGGTGGT 
TCTGCTCATG TTGAGCCCTG TGATGATTCC ACTCACATTT CTGTGCAAGA GGAAAACAAG 
TCGTCTGTCA GTCATTGCCT CCTTGATGGC TCTACAGTTC CTGAGGAAGG CTTATTTAGC 
CAAAAGAGTT TCCTTGTTTT GGGTTTTAGT GTTGAAAATG AATGTAATAT TGTAAACATC 
ATAAGAGAGC ATGCTGGAAA GATTGTGTCC CTTCCCAGCA GAATTGTTGC TGACTATGCC 
GTGGTTCCCC TGCTGGGATG TGAAGTAGAA GCGACAGTGG CAGAAGTTGT TACAAACACA 
TGGCTGGTTA CATGCATAGA CAACCAGACA TTAGTTGATC CCAAGTCGAA TCCCCTCTTC 
ACGCCAGTCT CAGTAATGTC AGGAATGACT CCTTTAGAGG ACTGTGTGAT TTCCTTCAGT 
CAGTGTGTGG GAGCAGAAAG AGATTCCTTG GTATTCTTAG CAAATCATCT TGGAGCAAGT 
GTTCAAGAAT TCTTTGTCCG CAAATCCAAT GCAAAGAAAG GCATGCTCGC CAGTACACAT 
CTTATAGTGA AAGAACCGAC TGGTTCTAAA TATGAAGCTG CAAAGAAATG GAGTTTGCCA 
GCAGTTAATA TTTCGTGGCT CTTAGAAACT GCTAGAATGG GGAAGAGAGC AGATGAAAAC 
CATTTTCTGG TTGACAGTGC ACCTAAACAA GAACAAGTAT TAGAAACTAA CATACCAAAT 
GGAGTGAGTC CAAATCCAGA TTCTCCAGCA CATCCTGATG CACACTTGGA AATTCACAGA 
CAAAAGGCCG TTACACCTTT GGACATGAAC CGATTTCAGA GTAAAGCTTT CCATGCTGTC 
ATCTCACAGC ACAACAGGCA GGGCTTGGCC TCCCCACCAG TGAGACAGCC ACCTAAGAAG 
GAACCCTCCT TCCACCTGGA CACACCCTCA AAGTTCCTGT CCAAGGACAA GCTTTTCAAG 
CCATCCTTTG ATGTGACGGA TGCACTTGCA GCCTTAGAAA CCCCAAATGC TGCCAGCCAG 
AAGCGGAAAC TGAGCTCACC ACTCTCTGAA GTCATCGTCA GAAACCTGAC AACTGCTTTG 
GCAAATAGTT CACGAAACAC CGGCTGTCTT TCTGCTAGCC CTCAGCTAAA GGGTGCCCAT 
TTGGAGAAGG AAGAGACTCC GAAACCGCTT GACAAAGTTG TGGTGTGCGT GAGTAAAAGG 
CTCAGTAAGA AGCAGAGTGA GCTAAATGGG GTTGCAGCCT CACTGGGAGC AGAGTACAGG 
TGGAGTTTTG ATGAGACAGT TACTCATTAC ATTTACCAGG GTCGGGCAAA TGATAGTAAT 
CGCGAGTATA AATCTGCAAA GGAAAGAGGG GTGCACGTCG TGTCGGAGCA TTGGCTGTTA 
GAGTGTGCTC AAGAGTATAA GCATCTTCCA GAATCTCTTT ACCCACACAC TTACAACCCC 
AAAATGAGCT TGGACATCAG CACAGTGCAG GACGGGCGGC TCTGTGACAG CCGCTTACCC 
TCGGCACTTT CTGCATCTAA GGACGATGAG CCAGATCACC TGTCTGTAGA AGAAAATGCA 
ACAGAGACTT TGGGCACACA CAGCAGAGAG TCCGCTCTGC TGAATGGGAA TGGAAGGGAC 
GACTGCAAAG GAGCTCTGAC CCAGGCCTTG GAGATGAGAG AGAACTTCCA GAAACAACTA 
CAAGAGATCA TGTCAGCAAC CTCCATCGTG AGGACACCAG CCCAGAAGAC TTGCATGTCA 
AGAAGTAGTT GTAACAGCGC CTCTTCAACT CCTGACAGCA CTCGCTCTGC TCGGAGTGGA 
CGAAGCCGAG TCTTGGAGGC GCTCAGGCAG TCTCGACAGA TAGTGCCTGA TATCAACACA 
GAGCCCTCCC AGAATGAACA GATCATTTGG GATGACCCAA CAGCAAGGGA GGAGAGAGCA 
AGGCTTGCCA GCAACCTACA GTGGCCTAGC TACCCAACAC AGTATTCCGA GCTTCAGGTT 
GAGATAAAAA AACTGGATGA TTCTCCTTCT CGAAAGCCTG TGTACCATTC AGAAATTGCT 
GGGCAGGCCT CGTGTGTGAC TCAAGCCCCA GGGCAGCACC CAGGCTCTGA AGAACCAGAA 
GCTCCAGCAG CAGAAAGGAA TCTGATCCCT GAGCCTCAAG CCCCCAGCGT GGCTTTCCCC 
CTGGCTAAGC CGCCTGTAGC TCCACAACCT GAGGACAAGA TTGAAACACT GGAGGAGACG 
CATGGAAAAC TTAAAAAACA GTATGTGTTC CAGATGTCAT CTCTGAATTC TCAAGAGCGG 
ATTGATTACT GTCGCCTGAT TAAAGACCTA GGCCCGGGAG TGCCCAGTGT GATAGTGCAC 
ACATGGAGCG CAGAACAGGT TCCGGGGAGG ACTCCCACCT GTCTTGGCCA AAATAAAAAG 
ATCCATGGAC TCTGCAGTCA GAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Topbp1 ( XM_008766589.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008764811.1 Rattus norvegicus topoisomerase (DNA) II binding protein 1 (Topbp1), transcript variant X2, mRNA.

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CloneID	ORa47492
Clone ID Related Accession (Same CDS sequence)	XM_008757856.2 , XM_006243684.2 , XM_008766589.2
Accession Version	XM_008766589.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3987bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	DNA topoisomerase 2-binding protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005107.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008766589.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAGAA ATGACCAAGA GCCGTTTTTT GTGAAGTTTT TAAAGTCTTC GGACAATTCA 
GAATGTTTTT TTAAAGCTCT TGAGTCAATA AAGGAGTTAC AATCAGAAGA GTATCTTCAG 
GTTATTACCG ATGAGGAAGC ATTGAGGATT AAAGAGAATG ACAAATCGCT TTATATCTGT 
GACCGTTTCA GTGGCACTGC TTTTGATCAC CTTAAACAGC TTGGCTGCAG AATTGTTGGT 
CCTCAAGTAG TCACATTTTG TATGCGCCAC CAGCAGTGTG TCCCAAGAGC TGAACATCCA 
GTTTATAATA TGATTATGTC TGACATAACT ATATCTTGTA CAAGCCTGGA TAAAGACAAG 
AGGGAAGAAG TTCATAAATA CGTACAAATG ATGGGTGGAC GAGTATACAG AGACCTTAAT 
GTCTCAGTCA CTCACCTTAT TGCAGGAGAA GTTGGGAGCA AAAAATACTT AGTTGCTGCT 
AATCTGAAGA AACCTATTTT GCTTCCTGCT TGGATTAAAA CACTTTGGGA GAAGTCACAA 
GAAAAAAAAA TAACCAAATA CACTGATGTA AACATGGAAG ACTTTAAGTG TCCTATTTTC 
CTTGGTTGCA TTATCTGTGT GACTGGCTTA AATGGTATAC ACAGGAAGAC AGTCCAGCAG 
CTCACAGCTA AGCATGGAGG CCAGTACATG GGGCAGCTGA AGATGAACGA GTGCACACAC 
CTCATTGTGC AAGAACCAAA AGGACAAAAA TATGAATGTG CCAGGAGATG GAATGTGCAC 
TGTGTGACCC TGCAATGGTT CCATGACAGT ATTGAGAAAG GCTTTTGTCA GGATGAGTCT 
ATATACAAGG CCGAACCCAG AGCAGCGGTA AAGACGGTGC CTGATACTTC CACACCCACT 
GCTCAGAGCG GCAGTGCTGC AGCTGAGAAT CACAGGCTGG CAGATGTCAG CCATATATCC 
AACATCAACG GCAGCTGTGT AAATGAAACA ATGTTTGGTT CAACTACGAG CAAACTCGAA 
TGCACACTTG AGAACCTAGA AAATCTGGAC ATCAGTATGT TTCAAGCACC TGAAGATCTA 
TTAGATGGCT GTCGGATATA TCTTTGTGGC TTTAGTGGCA GAAAGCTCGA TAAGTTGAGA 
AGGCTTATTA ACAGTGGAGG TGGAGTTCGG TTTAATCAAC TCAATGAAGA TGTAACTCAT 
GTAATCATGG GAGATTATGA GGATGACGTG AGGCAGTTTT GGAGCAAATC CTCCCACAGG 
CCCCATGTAG TGGGAGCAAA GTGGTTGCTG GAGTGTTTTA GTAAAGGATA TATACTTCCT 
GAAGAGTCAT ATATCCACGC TAACTACCAG CCAGATGGAA TTCCAGTTTC AGATCAGCCC 
AGAAACCAGA CAACTGTCTT AGAAAAGAGT GGCACATTCT CTAAGAAGGA CTTTGTTCCT 
AATGAGAAGT TTCAGCAGAC TGATGAGGAT CTGCTTGCTC AGTATGTACA TGATGACTCC 
ACAATAGTTG AAGCTAAGCT ATCTGAAGCT ATAGAGCCTG AAGTTGGGCC TTGCGGTGGT 
TCTGCTCATG TTGAGCCCTG TGATGATTCC ACTCACATTT CTGTGCAAGA GGAAAACAAG 
TCGTCTGTCA GTCATTGCCT CCTTGATGGC TCTACAGTTC CTGAGGAAGG CTTATTTAGC 
CAAAAGAGTT TCCTTGTTTT GGGTTTTAGT GTTGAAAATG AATGTAATAT TGTAAACATC 
ATAAGAGAGC ATGCTGGAAA GATTGTGTCC CTTCCCAGCA GAATTGTTGC TGACTATGCC 
GTGGTTCCCC TGCTGGGATG TGAAGTAGAA GCGACAGTGG CAGAAGTTGT TACAAACACA 
TGGCTGGTTA CATGCATAGA CAACCAGACA TTAGTTGATC CCAAGTCGAA TCCCCTCTTC 
ACGCCAGTCT CAGTAATGTC AGGAATGACT CCTTTAGAGG ACTGTGTGAT TTCCTTCAGT 
CAGTGTGTGG GAGCAGAAAG AGATTCCTTG GTATTCTTAG CAAATCATCT TGGAGCAAGT 
GTTCAAGAAT TCTTTGTCCG CAAATCCAAT GCAAAGAAAG GCATGCTCGC CAGTACACAT 
CTTATAGTGA AAGAACCGAC TGGTTCTAAA TATGAAGCTG CAAAGAAATG GAGTTTGCCA 
GCAGTTAATA TTTCGTGGCT CTTAGAAACT GCTAGAATGG GGAAGAGAGC AGATGAAAAC 
CATTTTCTGG TTGACAGTGC ACCTAAACAA GAACAAGTAT TAGAAACTAA CATACCAAAT 
GGAGTGAGTC CAAATCCAGA TTCTCCAGCA CATCCTGATG CACACTTGGA AATTCACAGA 
CAAAAGGCCG TTACACCTTT GGACATGAAC CGATTTCAGA GTAAAGCTTT CCATGCTGTC 
ATCTCACAGC ACAACAGGCA GGGCTTGGCC TCCCCACCAG TGAGACAGCC ACCTAAGAAG 
GAACCCTCCT TCCACCTGGA CACACCCTCA AAGTTCCTGT CCAAGGACAA GCTTTTCAAG 
CCATCCTTTG ATGTGACGGA TGCACTTGCA GCCTTAGAAA CCCCAAATGC TGCCAGCCAG 
AAGCGGAAAC TGAGCTCACC ACTCTCTGAA GTCATCGTCA GAAACCTGAC AACTGCTTTG 
GCAAATAGTT CACGAAACAC CGGCTGTCTT TCTGCTAGCC CTCAGCTAAA GGGTGCCCAT 
TTGGAGAAGG AAGAGACTCC GAAACCGCTT GACAAAGTTG TGGTGTGCGT GAGTAAAAGG 
CTCAGTAAGA AGCAGAGTGA GCTAAATGGG GTTGCAGCCT CACTGGGAGC AGAGTACAGG 
TGGAGTTTTG ATGAGACAGT TACTCATTAC ATTTACCAGG GTCGGGCAAA TGATAGTAAT 
CGCGAGTATA AATCTGCAAA GGAAAGAGGG GTGCACGTCG TGTCGGAGCA TTGGCTGTTA 
GAGTGTGCTC AAGAGTATAA GCATCTTCCA GAATCTCTTT ACCCACACAC TTACAACCCC 
AAAATGAGCT TGGACATCAG CACAGTGCAG GACGGGCGGC TCTGTGACAG CCGCTTACCC 
TCGGCACTTT CTGCATCTAA GGACGATGAG CCAGATCACC TGTCTGTAGA AGAAAATGCA 
ACAGAGACTT TGGGCACACA CAGCAGAGAG TCCGCTCTGC TGAATGGGAA TGGAAGGGAC 
GACTGCAAAG GAGCTCTGAC CCAGGCCTTG GAGATGAGAG AGAACTTCCA GAAACAACTA 
CAAGAGATCA TGTCAGCAAC CTCCATCGTG AGGACACCAG CCCAGAAGAC TTGCATGTCA 
AGAAGTAGTT GTAACAGCGC CTCTTCAACT CCTGACAGCA CTCGCTCTGC TCGGAGTGGA 
CGAAGCCGAG TCTTGGAGGC GCTCAGGCAG TCTCGACAGA TAGTGCCTGA TATCAACACA 
GAGCCCTCCC AGAATGAACA GATCATTTGG GATGACCCAA CAGCAAGGGA GGAGAGAGCA 
AGGCTTGCCA GCAACCTACA GTGGCCTAGC TACCCAACAC AGTATTCCGA GCTTCAGGTT 
GAGATAAAAA AACTGGATGA TTCTCCTTCT CGAAAGCCTG TGTACCATTC AGAAATTGCT 
GGGCAGGCCT CGTGTGTGAC TCAAGCCCCA GGGCAGCACC CAGGCTCTGA AGAACCAGAA 
GCTCCAGCAG CAGAAAGGAA TCTGATCCCT GAGCCTCAAG CCCCCAGCGT GGCTTTCCCC 
CTGGCTAAGC CGCCTGTAGC TCCACAACCT GAGGACAAGA TTGAAACACT GGAGGAGACG 
CATGGAAAAC TTAAAAAACA GTATGTGTTC CAGATGTCAT CTCTGAATTC TCAAGAGCGG 
ATTGATTACT GTCGCCTGAT TAAAGACCTA GGCCCGGGAG TGCCCAGTGT GATAGTGCAC 
ACATGGAGCG CAGAACAGGT TCCGGGGAGG ACTCCCACCT GTCTTGGCCA AAATAAAAAG 
ATCCATGGAC TCTGCAGTCA GAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008764811.1
CDS	71..4057
Translation	MSRNDQEPFFVKFLKSSDNSECFFKALESIKELQSEEYLQVITDEEALRIKENDKSLYICDRFSGTAFDHLKQLGCRIVGPQVVTFCMRHQQCVPRAEHPVYNMIMSDITISCTSLDKDKREEVHKYVQMMGGRVYRDLNVSVTHLIAGEVGSKKYLVAANLKKPILLPAWIKTLWEKSQEKKITKYTDVNMEDFKCPIFLGCIICVTGLNGIHRKTVQQLTAKHGGQYMGQLKMNECTHLIVQEPKGQKYECARRWNVHCVTLQWFHDSIEKGFCQDESIYKAEPRAAVKTVPDTSTPTAQSGSAAAENHRLADVSHISNINGSCVNETMFGSTTSKLECTLENLENLDISMFQAPEDLLDGCRIYLCGFSGRKLDKLRRLINSGGGVRFNQLNEDVTHVIMGDYEDDVRQFWSKSSHRPHVVGAKWLLECFSKGYILPEESYIHANYQPDGIPVSDQPRNQTTVLEKSGTFSKKDFVPNEKFQQTDEDLLAQYVHDDSTIVEAKLSEAIEPEVGPCGGSAHVEPCDDSTHISVQEENKSSVSHCLLDGSTVPEEGLFSQKSFLVLGFSVENECNIVNIIREHAGKIVSLPSRIVADYAVVPLLGCEVEATVAEVVTNTWLVTCIDNQTLVDPKSNPLFTPVSVMSGMTPLEDCVISFSQCVGAERDSLVFLANHLGASVQEFFVRKSNAKKGMLASTHLIVKEPTGSKYEAAKKWSLPAVNISWLLETARMGKRADENHFLVDSAPKQEQVLETNIPNGVSPNPDSPAHPDAHLEIHRQKAVTPLDMNRFQSKAFHAVISQHNRQGLASPPVRQPPKKEPSFHLDTPSKFLSKDKLFKPSFDVTDALAALETPNAASQKRKLSSPLSEVIVRNLTTALANSSRNTGCLSASPQLKGAHLEKEETPKPLDKVVVCVSKRLSKKQSELNGVAASLGAEYRWSFDETVTHYIYQGRANDSNREYKSAKERGVHVVSEHWLLECAQEYKHLPESLYPHTYNPKMSLDISTVQDGRLCDSRLPSALSASKDDEPDHLSVEENATETLGTHSRESALLNGNGRDDCKGALTQALEMRENFQKQLQEIMSATSIVRTPAQKTCMSRSSCNSASSTPDSTRSARSGRSRVLEALRQSRQIVPDINTEPSQNEQIIWDDPTAREERARLASNLQWPSYPTQYSELQVEIKKLDDSPSRKPVYHSEIAGQASCVTQAPGQHPGSEEPEAPAAERNLIPEPQAPSVAFPLAKPPVAPQPEDKIETLEETHGKLKKQYVFQMSSLNSQERIDYCRLIKDLGPGVPSVIVHTWSAEQVPGRTPTCLGQNKKIHGLCSQK

Target ORF information:

RefSeq Version	XM_008766589.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus topoisomerase (DNA) II binding protein 1 (Topbp1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008766589.2

ORF Insert Sequence:

ATGTCCAGAA ATGACCAAGA GCCGTTTTTT GTGAAGTTTT TAAAGTCTTC GGACAATTCA 
GAATGTTTTT TTAAAGCTCT TGAGTCAATA AAGGAGTTAC AATCAGAAGA GTATCTTCAG 
GTTATTACCG ATGAGGAAGC ATTGAGGATT AAAGAGAATG ACAAATCGCT TTATATCTGT 
GACCGTTTCA GTGGCACTGC TTTTGATCAC CTTAAACAGC TTGGCTGCAG AATTGTTGGT 
CCTCAAGTAG TCACATTTTG TATGCGCCAC CAGCAGTGTG TCCCAAGAGC TGAACATCCA 
GTTTATAATA TGATTATGTC TGACATAACT ATATCTTGTA CAAGCCTGGA TAAAGACAAG 
AGGGAAGAAG TTCATAAATA CGTACAAATG ATGGGTGGAC GAGTATACAG AGACCTTAAT 
GTCTCAGTCA CTCACCTTAT TGCAGGAGAA GTTGGGAGCA AAAAATACTT AGTTGCTGCT 
AATCTGAAGA AACCTATTTT GCTTCCTGCT TGGATTAAAA CACTTTGGGA GAAGTCACAA 
GAAAAAAAAA TAACCAAATA CACTGATGTA AACATGGAAG ACTTTAAGTG TCCTATTTTC 
CTTGGTTGCA TTATCTGTGT GACTGGCTTA AATGGTATAC ACAGGAAGAC AGTCCAGCAG 
CTCACAGCTA AGCATGGAGG CCAGTACATG GGGCAGCTGA AGATGAACGA GTGCACACAC 
CTCATTGTGC AAGAACCAAA AGGACAAAAA TATGAATGTG CCAGGAGATG GAATGTGCAC 
TGTGTGACCC TGCAATGGTT CCATGACAGT ATTGAGAAAG GCTTTTGTCA GGATGAGTCT 
ATATACAAGG CCGAACCCAG AGCAGCGGTA AAGACGGTGC CTGATACTTC CACACCCACT 
GCTCAGAGCG GCAGTGCTGC AGCTGAGAAT CACAGGCTGG CAGATGTCAG CCATATATCC 
AACATCAACG GCAGCTGTGT AAATGAAACA ATGTTTGGTT CAACTACGAG CAAACTCGAA 
TGCACACTTG AGAACCTAGA AAATCTGGAC ATCAGTATGT TTCAAGCACC TGAAGATCTA 
TTAGATGGCT GTCGGATATA TCTTTGTGGC TTTAGTGGCA GAAAGCTCGA TAAGTTGAGA 
AGGCTTATTA ACAGTGGAGG TGGAGTTCGG TTTAATCAAC TCAATGAAGA TGTAACTCAT 
GTAATCATGG GAGATTATGA GGATGACGTG AGGCAGTTTT GGAGCAAATC CTCCCACAGG 
CCCCATGTAG TGGGAGCAAA GTGGTTGCTG GAGTGTTTTA GTAAAGGATA TATACTTCCT 
GAAGAGTCAT ATATCCACGC TAACTACCAG CCAGATGGAA TTCCAGTTTC AGATCAGCCC 
AGAAACCAGA CAACTGTCTT AGAAAAGAGT GGCACATTCT CTAAGAAGGA CTTTGTTCCT 
AATGAGAAGT TTCAGCAGAC TGATGAGGAT CTGCTTGCTC AGTATGTACA TGATGACTCC 
ACAATAGTTG AAGCTAAGCT ATCTGAAGCT ATAGAGCCTG AAGTTGGGCC TTGCGGTGGT 
TCTGCTCATG TTGAGCCCTG TGATGATTCC ACTCACATTT CTGTGCAAGA GGAAAACAAG 
TCGTCTGTCA GTCATTGCCT CCTTGATGGC TCTACAGTTC CTGAGGAAGG CTTATTTAGC 
CAAAAGAGTT TCCTTGTTTT GGGTTTTAGT GTTGAAAATG AATGTAATAT TGTAAACATC 
ATAAGAGAGC ATGCTGGAAA GATTGTGTCC CTTCCCAGCA GAATTGTTGC TGACTATGCC 
GTGGTTCCCC TGCTGGGATG TGAAGTAGAA GCGACAGTGG CAGAAGTTGT TACAAACACA 
TGGCTGGTTA CATGCATAGA CAACCAGACA TTAGTTGATC CCAAGTCGAA TCCCCTCTTC 
ACGCCAGTCT CAGTAATGTC AGGAATGACT CCTTTAGAGG ACTGTGTGAT TTCCTTCAGT 
CAGTGTGTGG GAGCAGAAAG AGATTCCTTG GTATTCTTAG CAAATCATCT TGGAGCAAGT 
GTTCAAGAAT TCTTTGTCCG CAAATCCAAT GCAAAGAAAG GCATGCTCGC CAGTACACAT 
CTTATAGTGA AAGAACCGAC TGGTTCTAAA TATGAAGCTG CAAAGAAATG GAGTTTGCCA 
GCAGTTAATA TTTCGTGGCT CTTAGAAACT GCTAGAATGG GGAAGAGAGC AGATGAAAAC 
CATTTTCTGG TTGACAGTGC ACCTAAACAA GAACAAGTAT TAGAAACTAA CATACCAAAT 
GGAGTGAGTC CAAATCCAGA TTCTCCAGCA CATCCTGATG CACACTTGGA AATTCACAGA 
CAAAAGGCCG TTACACCTTT GGACATGAAC CGATTTCAGA GTAAAGCTTT CCATGCTGTC 
ATCTCACAGC ACAACAGGCA GGGCTTGGCC TCCCCACCAG TGAGACAGCC ACCTAAGAAG 
GAACCCTCCT TCCACCTGGA CACACCCTCA AAGTTCCTGT CCAAGGACAA GCTTTTCAAG 
CCATCCTTTG ATGTGACGGA TGCACTTGCA GCCTTAGAAA CCCCAAATGC TGCCAGCCAG 
AAGCGGAAAC TGAGCTCACC ACTCTCTGAA GTCATCGTCA GAAACCTGAC AACTGCTTTG 
GCAAATAGTT CACGAAACAC CGGCTGTCTT TCTGCTAGCC CTCAGCTAAA GGGTGCCCAT 
TTGGAGAAGG AAGAGACTCC GAAACCGCTT GACAAAGTTG TGGTGTGCGT GAGTAAAAGG 
CTCAGTAAGA AGCAGAGTGA GCTAAATGGG GTTGCAGCCT CACTGGGAGC AGAGTACAGG 
TGGAGTTTTG ATGAGACAGT TACTCATTAC ATTTACCAGG GTCGGGCAAA TGATAGTAAT 
CGCGAGTATA AATCTGCAAA GGAAAGAGGG GTGCACGTCG TGTCGGAGCA TTGGCTGTTA 
GAGTGTGCTC AAGAGTATAA GCATCTTCCA GAATCTCTTT ACCCACACAC TTACAACCCC 
AAAATGAGCT TGGACATCAG CACAGTGCAG GACGGGCGGC TCTGTGACAG CCGCTTACCC 
TCGGCACTTT CTGCATCTAA GGACGATGAG CCAGATCACC TGTCTGTAGA AGAAAATGCA 
ACAGAGACTT TGGGCACACA CAGCAGAGAG TCCGCTCTGC TGAATGGGAA TGGAAGGGAC 
GACTGCAAAG GAGCTCTGAC CCAGGCCTTG GAGATGAGAG AGAACTTCCA GAAACAACTA 
CAAGAGATCA TGTCAGCAAC CTCCATCGTG AGGACACCAG CCCAGAAGAC TTGCATGTCA 
AGAAGTAGTT GTAACAGCGC CTCTTCAACT CCTGACAGCA CTCGCTCTGC TCGGAGTGGA 
CGAAGCCGAG TCTTGGAGGC GCTCAGGCAG TCTCGACAGA TAGTGCCTGA TATCAACACA 
GAGCCCTCCC AGAATGAACA GATCATTTGG GATGACCCAA CAGCAAGGGA GGAGAGAGCA 
AGGCTTGCCA GCAACCTACA GTGGCCTAGC TACCCAACAC AGTATTCCGA GCTTCAGGTT 
GAGATAAAAA AACTGGATGA TTCTCCTTCT CGAAAGCCTG TGTACCATTC AGAAATTGCT 
GGGCAGGCCT CGTGTGTGAC TCAAGCCCCA GGGCAGCACC CAGGCTCTGA AGAACCAGAA 
GCTCCAGCAG CAGAAAGGAA TCTGATCCCT GAGCCTCAAG CCCCCAGCGT GGCTTTCCCC 
CTGGCTAAGC CGCCTGTAGC TCCACAACCT GAGGACAAGA TTGAAACACT GGAGGAGACG 
CATGGAAAAC TTAAAAAACA GTATGTGTTC CAGATGTCAT CTCTGAATTC TCAAGAGCGG 
ATTGATTACT GTCGCCTGAT TAAAGACCTA GGCCCGGGAG TGCCCAGTGT GATAGTGCAC 
ACATGGAGCG CAGAACAGGT TCCGGGGAGG ACTCCCACCT GTCTTGGCCA AAATAAAAAG 
ATCCATGGAC TCTGCAGTCA GAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Topbp1 ( XM_006226542.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006226604.1 Rattus norvegicus topoisomerase (DNA) II binding protein 1 (Topbp1), transcript variant X6, mRNA.

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CloneID	ORa18094
Clone ID Related Accession (Same CDS sequence)	XM_006226542.2
Accession Version	XM_006226542.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4200bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	DNA topoisomerase 2-binding protein 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (AC_000076.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006226542.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGAAG TTCATAAATA CGTACAAATG ATGGGTGGAC GAGTATACAG AGACCTTAAT 
GTCTCAGTCA CTCACCTTAT TGCAGGAGAA GTTGGGAGCA AAAAATACTT AGTTGCTGCT 
AATCTGAAGA AACCTATTTT GCTTCCTGCT TGGATTAAAA CACTTTGGGA GAAGTCACAA 
GAAAAAAAAA TAACCAAATA CACTGATGTA AACATGGAAG ACTTTAAGTG TCCTATTTTC 
CTTGGTTGCA TTATCTGTGT GACTGGCTTA AATGGTATAC ACAGGAAGAC AGTCCAGCAG 
CTCACAGCTA AGCATGGAGG CCAGTACATG GGGCAGCTGA AGATGAACGA GTGCACACAC 
CTCATTGTGC AAGAACCAAA AGGACAAAAA TATGAATGTG CCAGGAGATG GAATGTGCAC 
TGTGTGACCC TGCAATGGTT CCATGACAGT ATTGAGAAAG GCTTTTGTCA GGATGAGTCT 
ATATACAAGG CCGAACCCAG AGCAGCGGTA AAGACGGTGC CTGATACTTC CACACCCACT 
GCTCAGAGCG GCAGTGCTGC AGCTGAGAAT CACAGGCTGG CAGATGTCAG CCATATATCC 
AACATCAACG GCAGCTGTGT AAATGAAACA ATGTTTGGTT CAACTACGAG CAAACTCGAA 
TGCACACTTG AGAACCTAGA AAATCTGGAC ATCAGTATGT TTCAAGCACC TGAAGATCTA 
TTAGATGGCT GTCGGATATA TCTTTGTGGC TTTAGTGGCA GAAAGCTCGA TAAGTTGAGA 
AGGCTTATTA ACAGTGGAGG TGGAGTTCGG TTTAATCAAC TCAATGAAGA TGTAACTCAT 
GTAATCATGG GAGATTATGA GGATGACGTG AGGCAGTTTT GGAGCAAATC CTCCCACAGG 
CCCCATGTAG TGGGAGCAAA GTGGTTGCTG GAGTGTTTTA GTAAAGGATA TATACTTCCT 
GAAGAGTCAT ATATCCACGC TAACTACCAG CCAGATGGAA TTCCAGTTTC AGATCAGCCC 
AGAAACCAGA CAACTGTCTT AGAAAAGAGT GGCACATTCT CTAAGAAGGA CTTTGTTCCT 
AATGAGAAGT TTCAGCAGAC TGATGAGGAT CTGCTTGCTC AGTATGTACA TGATGACTCC 
ACAATAGTTG AAGCTAAGCT ATCTGAAGCT ATAGAGCCTG AAGTTGGGCC TTGCGGTGGT 
TCTGCTCATG TTGAGCCCTG TGATGATTCC ACTCACATTT CTGTGCAAGA GGAAAACAAG 
TCGTCTGTCA GTCATTGCCT CCTTGATGGC TCTACAGTTC CTGAGGAAGG CTTATTTAGC 
CAAAAGAGTT TCCTTGTTTT GGGTTTTAGT GTTGAAAATG AATGTAATAT TGTAAACATC 
ATAAGAGAGC ATGCTGGAAA GATTGTGTCC CTTCCCAGCA GAATTGTTGC TGACTATGCC 
GTGGTTCCCC TGCTGGGATG TGAAGTAGAA GCGACAGTGG CAGAAGTTGT TACAAACACA 
TGGCTGGTTA CATGCATAGA CAACCAGACA TTAGTTGATC CCAAGTCGAA TCCCCTCTTC 
ACGCCAGTCT CAGTAATGTC AGGAATGACT CCTTTAGAGG ACTGTGTGAT TTCCTTCAGT 
CAGTGTGTGG GAGCAGAAAG AGATTCCTTG GTATTCTTAG CAAATCATCT TGGAGCAAGT 
GTTCAAGAAT TCTTTGTCCG CAAATCCAAT GCAAAGAAAG GCATGCTCGC CAGTACACAT 
CTTATAGTGA AAGAACCGAC TGGTTCTAAA TATGAAGCTG CAAAGAAATG GAGTTTGCCA 
GCAGTTAATA TTTCGTGGCT CTTAGAAACT GCTAGAATGG GGAAGAGAGC AGATGAAAAC 
CATTTTCTGG TTGACAGTGC ACCTAAACAA GAACAAGTAT TAGAAACTAA CATACCAAAT 
GGAGTGAGTC CAAATCCAGA TTCTCCAGCA CATCCTGATG CACACTTGGA AATTCACAGA 
CAAAAGGCCG TTACACCTTT GGACATGAAC CGATTTCAGA GTAAAGCTTT CCATGCTGTC 
ATCTCACAGC ACAACAGGCA GGGCTTGGCC TCCCCACCAG TGAGACAGCC ACCTAAGAAG 
GAACCCTCCT TCCACCTGGA CACACCCTCA AAGTTCCTGT CCAAGGACAA GCTTTTCAAG 
CCATCCTTTG ATGTGACGGA TGCACTTGCA GCCTTAGAAA CCCCAAATGC TGCCAGCCAG 
AAGCGGAAAC TGAGCTCACC ACTCTCTGAA GTCATCGTCA GAAACCTGAC AACTGCTTTG 
GCAAATAGTT CACGAAACAC CGGCTGTCTT TCTGCTAGCC CTCAGCTAAA GGGTGCCCAT 
TTGGAGAAGG AAGAGACTCC GAAACCGCTT GACAAAGTTG TGGTGTGCGT GAGTAAAAGG 
CTCAGTAAGA AGCAGAGTGA GCTAAATGGG GTTGCAGCCT CACTGGGAGC AGAGTACAGG 
TGGAGTTTTG ATGAGACAGT TACTCATTAC ATTTACCAGG GTCGGGCAAA TGATAGTAAT 
CGCGAGTATA AATCTGCAAA GGAAAGAGGG GTGCACGTCG TGTCGGAGCA TTGGCTGTTA 
GAGTGTGCTC AAGAGTATAA GCATCTTCCA GAATCTCTTT ACCCACACAC TTACAACCCC 
AAAATGAGCT TGGACATCAG CACAGTGCAG GACGGGCGGC TCTGTGACAG CCGCTTACCC 
TCGGCACTTT CTGCATCTAA GGACGATGAG CCAGATCACC TGTCTGTAGA AGAAAATGCA 
ACAGAGACTT TGGGCACACA CAGCAGAGAG TCCGCTCTGC TGAATGGGAA TGGAAGGGAC 
GACTGCAAAG GAGCTCTGAC CCAGGCCTTG GAGATGAGAG AGAACTTCCA GAAACAACTA 
CAAGAGATCA TGTCAGCAAC CTCCATCGTG AGGACACCAG CCCAGAAGAC TTGCATGTCA 
AGAAGTAGTT GTAACAGCGC CTCTTCAACT CCTGACAGCA CTCGCTCTGC TCGGAGTGGA 
CGAAGCCGAG TCTTGGAGGC GCTCAGGCAG TCTCGACAGA TAGTGCCTGA TATCAACACA 
GAGCCCTCCC AGAATGAACA GATCATTTGG GATGACCCAA CAGCAAGGGA GGAGAGAGCA 
AGGCTTGCCA GCAACCTACA GTGGCCTAGC TACCCAACAC AGTATTCCGA GCTTCAGGTT 
GAGATAAAAA AACTGGATGA TTCTCCTTCT CGAAAGCCTG TGTACCATTC AGAAATTGCT 
GGGCAGGCCT CGTGTGTGAC TCAAGCCCCA GGGCAGCACC CAGGCTCTGA AGAACCAGAA 
GCTCCAGCAG CAGAAAGGAA TCTGATCCCT GAGCCTCAAG CCCCCAGCGT GGCTTTCCCC 
CTGGCTAAGC CGCCTGTAGC TCCACAACCT GAGGACAAGA TTGAAACACT GGAGGAGACG 
CATGGAAAAC TTAAAAAACA GTATGTGTTC CAGATGTCAT CTCTGAATTC TCAAGAGCGG 
ATTGATTACT GTCGCCTGAT TAAAGACCTA GGTGGGTCGG TGATCGAGAA GCAGTGCTCA 
GACCCCAGCT GCACACACAT GGTTGTGGGG TACCCACTGC GCAATGAGAA ATACTTAGCT 
TCCATGGCAG CTGGCAAATG GGTGCTTCAT CGCTCCTACC TGGACGCTTG CCGGACAGCT 
GGGCGCTTTG TGCAGGAGGA AGACTACGAG TGGGGAAGCA GCTCCATCCT AGATGCTCTC 
CCTGACGTCA CTGAGCACCA GCAGAAGCTC GCACTTGCAG CAATGAGATG GAGGAAAAGA 
ATCCAGCAGA GTCGGGAGTC GGGCATCGCT GAGGGAGCAT TTAGTGGCTG GAAAGTTATT 
TTGCGCGTTG ATCGGCCCCG AGAAGCAGGG TTTAAGCGCC TTCTTCAGGC AGGAGGAGCA 
AAGGTATTGC CTGGTCATCC AGAGCCTTTA TTTAAGGATG CTACACATCT CTTCTGTGAT 
TTCAATAAAC TGAAGCCTGA TGACTGCAGA GTTTTTATCG CAGAGGCTAC GGCCCAGAAC 
ATGGTCTGCT TGAAAACAGA ATATATTGCT GATTACCTCA TGATGGAATC CCCTCCTTGT 
GCTGATAATT ACCGAGTATC CGAAACTGCT TTATTCCGTA ACAAGGAAGG TGGGCCTGGA 
CTACCACAGA AGAGGAAAAC TCCTGCAGAG AACGTCATCA AACGGCCTCG GGTGCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006226604.1
CDS	124..4323
Translation	MEEVHKYVQMMGGRVYRDLNVSVTHLIAGEVGSKKYLVAANLKKPILLPAWIKTLWEKSQEKKITKYTDVNMEDFKCPIFLGCIICVTGLNGIHRKTVQQLTAKHGGQYMGQLKMNECTHLIVQEPKGQKYECARRWNVHCVTLQWFHDSIEKGFCQDESIYKAEPRAAVKTVPDTSTPTAQSGSAAAENHRLADVSHISNINGSCVNETMFGSTTSKLECTLENLENLDISMFQAPEDLLDGCRIYLCGFSGRKLDKLRRLINSGGGVRFNQLNEDVTHVIMGDYEDDVRQFWSKSSHRPHVVGAKWLLECFSKGYILPEESYIHANYQPDGIPVSDQPRNQTTVLEKSGTFSKKDFVPNEKFQQTDEDLLAQYVHDDSTIVEAKLSEAIEPEVGPCGGSAHVEPCDDSTHISVQEENKSSVSHCLLDGSTVPEEGLFSQKSFLVLGFSVENECNIVNIIREHAGKIVSLPSRIVADYAVVPLLGCEVEATVAEVVTNTWLVTCIDNQTLVDPKSNPLFTPVSVMSGMTPLEDCVISFSQCVGAERDSLVFLANHLGASVQEFFVRKSNAKKGMLASTHLIVKEPTGSKYEAAKKWSLPAVNISWLLETARMGKRADENHFLVDSAPKQEQVLETNIPNGVSPNPDSPAHPDAHLEIHRQKAVTPLDMNRFQSKAFHAVISQHNRQGLASPPVRQPPKKEPSFHLDTPSKFLSKDKLFKPSFDVTDALAALETPNAASQKRKLSSPLSEVIVRNLTTALANSSRNTGCLSASPQLKGAHLEKEETPKPLDKVVVCVSKRLSKKQSELNGVAASLGAEYRWSFDETVTHYIYQGRANDSNREYKSAKERGVHVVSEHWLLECAQEYKHLPESLYPHTYNPKMSLDISTVQDGRLCDSRLPSALSASKDDEPDHLSVEENATETLGTHSRESALLNGNGRDDCKGALTQALEMRENFQKQLQEIMSATSIVRTPAQKTCMSRSSCNSASSTPDSTRSARSGRSRVLEALRQSRQIVPDINTEPSQNEQIIWDDPTAREERARLASNLQWPSYPTQYSELQVEIKKLDDSPSRKPVYHSEIAGQASCVTQAPGQHPGSEEPEAPAAERNLIPEPQAPSVAFPLAKPPVAPQPEDKIETLEETHGKLKKQYVFQMSSLNSQERIDYCRLIKDLGGSVIEKQCSDPSCTHMVVGYPLRNEKYLASMAAGKWVLHRSYLDACRTAGRFVQEEDYEWGSSSILDALPDVTEHQQKLALAAMRWRKRIQQSRESGIAEGAFSGWKVILRVDRPREAGFKRLLQAGGAKVLPGHPEPLFKDATHLFCDFNKLKPDDCRVFIAEATAQNMVCLKTEYIADYLMMESPPCADNYRVSETALFRNKEGGPGLPQKRKTPAENVIKRPRVH

Target ORF information:

RefSeq Version	XM_006226542.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus topoisomerase (DNA) II binding protein 1 (Topbp1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006226542.2

ORF Insert Sequence:

ATGGAAGAAG TTCATAAATA CGTACAAATG ATGGGTGGAC GAGTATACAG AGACCTTAAT 
GTCTCAGTCA CTCACCTTAT TGCAGGAGAA GTTGGGAGCA AAAAATACTT AGTTGCTGCT 
AATCTGAAGA AACCTATTTT GCTTCCTGCT TGGATTAAAA CACTTTGGGA GAAGTCACAA 
GAAAAAAAAA TAACCAAATA CACTGATGTA AACATGGAAG ACTTTAAGTG TCCTATTTTC 
CTTGGTTGCA TTATCTGTGT GACTGGCTTA AATGGTATAC ACAGGAAGAC AGTCCAGCAG 
CTCACAGCTA AGCATGGAGG CCAGTACATG GGGCAGCTGA AGATGAACGA GTGCACACAC 
CTCATTGTGC AAGAACCAAA AGGACAAAAA TATGAATGTG CCAGGAGATG GAATGTGCAC 
TGTGTGACCC TGCAATGGTT CCATGACAGT ATTGAGAAAG GCTTTTGTCA GGATGAGTCT 
ATATACAAGG CCGAACCCAG AGCAGCGGTA AAGACGGTGC CTGATACTTC CACACCCACT 
GCTCAGAGCG GCAGTGCTGC AGCTGAGAAT CACAGGCTGG CAGATGTCAG CCATATATCC 
AACATCAACG GCAGCTGTGT AAATGAAACA ATGTTTGGTT CAACTACGAG CAAACTCGAA 
TGCACACTTG AGAACCTAGA AAATCTGGAC ATCAGTATGT TTCAAGCACC TGAAGATCTA 
TTAGATGGCT GTCGGATATA TCTTTGTGGC TTTAGTGGCA GAAAGCTCGA TAAGTTGAGA 
AGGCTTATTA ACAGTGGAGG TGGAGTTCGG TTTAATCAAC TCAATGAAGA TGTAACTCAT 
GTAATCATGG GAGATTATGA GGATGACGTG AGGCAGTTTT GGAGCAAATC CTCCCACAGG 
CCCCATGTAG TGGGAGCAAA GTGGTTGCTG GAGTGTTTTA GTAAAGGATA TATACTTCCT 
GAAGAGTCAT ATATCCACGC TAACTACCAG CCAGATGGAA TTCCAGTTTC AGATCAGCCC 
AGAAACCAGA CAACTGTCTT AGAAAAGAGT GGCACATTCT CTAAGAAGGA CTTTGTTCCT 
AATGAGAAGT TTCAGCAGAC TGATGAGGAT CTGCTTGCTC AGTATGTACA TGATGACTCC 
ACAATAGTTG AAGCTAAGCT ATCTGAAGCT ATAGAGCCTG AAGTTGGGCC TTGCGGTGGT 
TCTGCTCATG TTGAGCCCTG TGATGATTCC ACTCACATTT CTGTGCAAGA GGAAAACAAG 
TCGTCTGTCA GTCATTGCCT CCTTGATGGC TCTACAGTTC CTGAGGAAGG CTTATTTAGC 
CAAAAGAGTT TCCTTGTTTT GGGTTTTAGT GTTGAAAATG AATGTAATAT TGTAAACATC 
ATAAGAGAGC ATGCTGGAAA GATTGTGTCC CTTCCCAGCA GAATTGTTGC TGACTATGCC 
GTGGTTCCCC TGCTGGGATG TGAAGTAGAA GCGACAGTGG CAGAAGTTGT TACAAACACA 
TGGCTGGTTA CATGCATAGA CAACCAGACA TTAGTTGATC CCAAGTCGAA TCCCCTCTTC 
ACGCCAGTCT CAGTAATGTC AGGAATGACT CCTTTAGAGG ACTGTGTGAT TTCCTTCAGT 
CAGTGTGTGG GAGCAGAAAG AGATTCCTTG GTATTCTTAG CAAATCATCT TGGAGCAAGT 
GTTCAAGAAT TCTTTGTCCG CAAATCCAAT GCAAAGAAAG GCATGCTCGC CAGTACACAT 
CTTATAGTGA AAGAACCGAC TGGTTCTAAA TATGAAGCTG CAAAGAAATG GAGTTTGCCA 
GCAGTTAATA TTTCGTGGCT CTTAGAAACT GCTAGAATGG GGAAGAGAGC AGATGAAAAC 
CATTTTCTGG TTGACAGTGC ACCTAAACAA GAACAAGTAT TAGAAACTAA CATACCAAAT 
GGAGTGAGTC CAAATCCAGA TTCTCCAGCA CATCCTGATG CACACTTGGA AATTCACAGA 
CAAAAGGCCG TTACACCTTT GGACATGAAC CGATTTCAGA GTAAAGCTTT CCATGCTGTC 
ATCTCACAGC ACAACAGGCA GGGCTTGGCC TCCCCACCAG TGAGACAGCC ACCTAAGAAG 
GAACCCTCCT TCCACCTGGA CACACCCTCA AAGTTCCTGT CCAAGGACAA GCTTTTCAAG 
CCATCCTTTG ATGTGACGGA TGCACTTGCA GCCTTAGAAA CCCCAAATGC TGCCAGCCAG 
AAGCGGAAAC TGAGCTCACC ACTCTCTGAA GTCATCGTCA GAAACCTGAC AACTGCTTTG 
GCAAATAGTT CACGAAACAC CGGCTGTCTT TCTGCTAGCC CTCAGCTAAA GGGTGCCCAT 
TTGGAGAAGG AAGAGACTCC GAAACCGCTT GACAAAGTTG TGGTGTGCGT GAGTAAAAGG 
CTCAGTAAGA AGCAGAGTGA GCTAAATGGG GTTGCAGCCT CACTGGGAGC AGAGTACAGG 
TGGAGTTTTG ATGAGACAGT TACTCATTAC ATTTACCAGG GTCGGGCAAA TGATAGTAAT 
CGCGAGTATA AATCTGCAAA GGAAAGAGGG GTGCACGTCG TGTCGGAGCA TTGGCTGTTA 
GAGTGTGCTC AAGAGTATAA GCATCTTCCA GAATCTCTTT ACCCACACAC TTACAACCCC 
AAAATGAGCT TGGACATCAG CACAGTGCAG GACGGGCGGC TCTGTGACAG CCGCTTACCC 
TCGGCACTTT CTGCATCTAA GGACGATGAG CCAGATCACC TGTCTGTAGA AGAAAATGCA 
ACAGAGACTT TGGGCACACA CAGCAGAGAG TCCGCTCTGC TGAATGGGAA TGGAAGGGAC 
GACTGCAAAG GAGCTCTGAC CCAGGCCTTG GAGATGAGAG AGAACTTCCA GAAACAACTA 
CAAGAGATCA TGTCAGCAAC CTCCATCGTG AGGACACCAG CCCAGAAGAC TTGCATGTCA 
AGAAGTAGTT GTAACAGCGC CTCTTCAACT CCTGACAGCA CTCGCTCTGC TCGGAGTGGA 
CGAAGCCGAG TCTTGGAGGC GCTCAGGCAG TCTCGACAGA TAGTGCCTGA TATCAACACA 
GAGCCCTCCC AGAATGAACA GATCATTTGG GATGACCCAA CAGCAAGGGA GGAGAGAGCA 
AGGCTTGCCA GCAACCTACA GTGGCCTAGC TACCCAACAC AGTATTCCGA GCTTCAGGTT 
GAGATAAAAA AACTGGATGA TTCTCCTTCT CGAAAGCCTG TGTACCATTC AGAAATTGCT 
GGGCAGGCCT CGTGTGTGAC TCAAGCCCCA GGGCAGCACC CAGGCTCTGA AGAACCAGAA 
GCTCCAGCAG CAGAAAGGAA TCTGATCCCT GAGCCTCAAG CCCCCAGCGT GGCTTTCCCC 
CTGGCTAAGC CGCCTGTAGC TCCACAACCT GAGGACAAGA TTGAAACACT GGAGGAGACG 
CATGGAAAAC TTAAAAAACA GTATGTGTTC CAGATGTCAT CTCTGAATTC TCAAGAGCGG 
ATTGATTACT GTCGCCTGAT TAAAGACCTA GGTGGGTCGG TGATCGAGAA GCAGTGCTCA 
GACCCCAGCT GCACACACAT GGTTGTGGGG TACCCACTGC GCAATGAGAA ATACTTAGCT 
TCCATGGCAG CTGGCAAATG GGTGCTTCAT CGCTCCTACC TGGACGCTTG CCGGACAGCT 
GGGCGCTTTG TGCAGGAGGA AGACTACGAG TGGGGAAGCA GCTCCATCCT AGATGCTCTC 
CCTGACGTCA CTGAGCACCA GCAGAAGCTC GCACTTGCAG CAATGAGATG GAGGAAAAGA 
ATCCAGCAGA GTCGGGAGTC GGGCATCGCT GAGGGAGCAT TTAGTGGCTG GAAAGTTATT 
TTGCGCGTTG ATCGGCCCCG AGAAGCAGGG TTTAAGCGCC TTCTTCAGGC AGGAGGAGCA 
AAGGTATTGC CTGGTCATCC AGAGCCTTTA TTTAAGGATG CTACACATCT CTTCTGTGAT 
TTCAATAAAC TGAAGCCTGA TGACTGCAGA GTTTTTATCG CAGAGGCTAC GGCCCAGAAC 
ATGGTCTGCT TGAAAACAGA ATATATTGCT GATTACCTCA TGATGGAATC CCCTCCTTGT 
GCTGATAATT ACCGAGTATC CGAAACTGCT TTATTCCGTA ACAAGGAAGG TGGGCCTGGA 
CTACCACAGA AGAGGAAAAC TCCTGCAGAG AACGTCATCA AACGGCCTCG GGTGCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Topbp1 ( XM_236578.8 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_236578.7 Rattus norvegicus topoisomerase (DNA) II binding protein 1 (Topbp1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ORa47491
Clone ID Related Accession (Same CDS sequence)	XM_236578.8 , XM_001072207.5 , XM_008757855.2
Accession Version	XM_236578.8 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4560bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	DNA topoisomerase 2-binding protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005107.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_236578.7. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAGAA ATGACCAAGA GCCGTTTTTT GTGAAGTTTT TAAAGTCTTC GGACAATTCA 
GAATGTTTTT TTAAAGCTCT TGAGTCAATA AAGGAGTTAC AATCAGAAGA GTATCTTCAG 
GTTATTACCG ATGAGGAAGC ATTGAGGATT AAAGAGAATG ACAAATCGCT TTATATCTGT 
GACCGTTTCA GTGGCACTGC TTTTGATCAC CTTAAACAGC TTGGCTGCAG AATTGTTGGT 
CCTCAAGTAG TCACATTTTG TATGCGCCAC CAGCAGTGTG TCCCAAGAGC TGAACATCCA 
GTTTATAATA TGATTATGTC TGACATAACT ATATCTTGTA CAAGCCTGGA TAAAGACAAG 
AGGGAAGAAG TTCATAAATA CGTACAAATG ATGGGTGGAC GAGTATACAG AGACCTTAAT 
GTCTCAGTCA CTCACCTTAT TGCAGGAGAA GTTGGGAGCA AAAAATACTT AGTTGCTGCT 
AATCTGAAGA AACCTATTTT GCTTCCTGCT TGGATTAAAA CACTTTGGGA GAAGTCACAA 
GAAAAAAAAA TAACCAAATA CACTGATGTA AACATGGAAG ACTTTAAGTG TCCTATTTTC 
CTTGGTTGCA TTATCTGTGT GACTGGCTTA AATGGTATAC ACAGGAAGAC AGTCCAGCAG 
CTCACAGCTA AGCATGGAGG CCAGTACATG GGGCAGCTGA AGATGAACGA GTGCACACAC 
CTCATTGTGC AAGAACCAAA AGGACAAAAA TATGAATGTG CCAGGAGATG GAATGTGCAC 
TGTGTGACCC TGCAATGGTT CCATGACAGT ATTGAGAAAG GCTTTTGTCA GGATGAGTCT 
ATATACAAGG CCGAACCCAG AGCAGCGGTA AAGACGGTGC CTGATACTTC CACACCCACT 
GCTCAGAGCG GCAGTGCTGC AGCTGAGAAT CACAGGCTGG CAGATGTCAG CCATATATCC 
AACATCAACG GCAGCTGTGT AAATGAAACA ATGTTTGGTT CAACTACGAG CAAACTCGAA 
TGCACACTTG AGAACCTAGA AAATCTGGAC ATCAGTATGT TTCAAGCACC TGAAGATCTA 
TTAGATGGCT GTCGGATATA TCTTTGTGGC TTTAGTGGCA GAAAGCTCGA TAAGTTGAGA 
AGGCTTATTA ACAGTGGAGG TGGAGTTCGG TTTAATCAAC TCAATGAAGA TGTAACTCAT 
GTAATCATGG GAGATTATGA GGATGACGTG AGGCAGTTTT GGAGCAAATC CTCCCACAGG 
CCCCATGTAG TGGGAGCAAA GTGGTTGCTG GAGTGTTTTA GTAAAGGATA TATACTTCCT 
GAAGAGTCAT ATATCCACGC TAACTACCAG CCAGATGGAA TTCCAGTTTC AGATCAGCCC 
AGAAACCAGA CAACTGTCTT AGAAAAGAGT GGCACATTCT CTAAGAAGGA CTTTGTTCCT 
AATGAGAAGT TTCAGCAGAC TGATGAGGAT CTGCTTGCTC AGTATGTACA TGATGACTCC 
ACAATAGTTG AAGCTAAGCT ATCTGAAGCT ATAGAGCCTG AAGTTGGGCC TTGCGGTGGT 
TCTGCTCATG TTGAGCCCTG TGATGATTCC ACTCACATTT CTGTGCAAGA GGAAAACAAG 
TCGTCTGTCA GTCATTGCCT CCTTGATGGC TCTACAGTTC CTGAGGAAGG CTTATTTAGC 
CAAAAGAGTT TCCTTGTTTT GGGTTTTAGT GTTGAAAATG AATGTAATAT TGTAAACATC 
ATAAGAGAGC ATGCTGGAAA GATTGTGTCC CTTCCCAGCA GAATTGTTGC TGACTATGCC 
GTGGTTCCCC TGCTGGGATG TGAAGTAGAA GCGACAGTGG CAGAAGTTGT TACAAACACA 
TGGCTGGTTA CATGCATAGA CAACCAGACA TTAGTTGATC CCAAGTCGAA TCCCCTCTTC 
ACGCCAGTCT CAGTAATGTC AGGAATGACT CCTTTAGAGG ACTGTGTGAT TTCCTTCAGT 
CAGTGTGTGG GAGCAGAAAG AGATTCCTTG GTATTCTTAG CAAATCATCT TGGAGCAAGT 
GTTCAAGAAT TCTTTGTCCG CAAATCCAAT GCAAAGAAAG GCATGCTCGC CAGTACACAT 
CTTATAGTGA AAGAACCGAC TGGTTCTAAA TATGAAGCTG CAAAGAAATG GAGTTTGCCA 
GCAGTTAATA TTTCGTGGCT CTTAGAAACT GCTAGAATGG GGAAGAGAGC AGATGAAAAC 
CATTTTCTGG TTGACAGTGC ACCTAAACAA GAACAAGTAT TAGAAACTAA CATACCAAAT 
GGAGTGAGTC CAAATCCAGA TTCTCCAGCA CATCCTGATG CACACTTGGA AATTCACAGA 
CAAAAGGCCG TTACACCTTT GGACATGAAC CGATTTCAGA GTAAAGCTTT CCATGCTGTC 
ATCTCACAGC ACAACAGGCA GGGCTTGGCC TCCCCACCAG TGAGACAGCC ACCTAAGAAG 
GAACCCTCCT TCCACCTGGA CACACCCTCA AAGTTCCTGT CCAAGGACAA GCTTTTCAAG 
CCATCCTTTG ATGTGACGGA TGCACTTGCA GCCTTAGAAA CCCCAAATGC TGCCAGCCAG 
AAGCGGAAAC TGAGCTCACC ACTCTCTGAA GTCATCGTCA GAAACCTGAC AACTGCTTTG 
GCAAATAGTT CACGAAACAC CGGCTGTCTT TCTGCTAGCC CTCAGCTAAA GGGTGCCCAT 
TTGGAGAAGG AAGAGACTCC GAAACCGCTT GACAAAGTTG TGGTGTGCGT GAGTAAAAGG 
CTCAGTAAGA AGCAGAGTGA GCTAAATGGG GTTGCAGCCT CACTGGGAGC AGAGTACAGG 
TGGAGTTTTG ATGAGACAGT TACTCATTAC ATTTACCAGG GTCGGGCAAA TGATAGTAAT 
CGCGAGTATA AATCTGCAAA GGAAAGAGGG GTGCACGTCG TGTCGGAGCA TTGGCTGTTA 
GAGTGTGCTC AAGAGTATAA GCATCTTCCA GAATCTCTTT ACCCACACAC TTACAACCCC 
AAAATGAGCT TGGACATCAG CACAGTGCAG GACGGGCGGC TCTGTGACAG CCGCTTACCC 
TCGGCACTTT CTGCATCTAA GGACGATGAG CCAGATCACC TGTCTGTAGA AGAAAATGCA 
ACAGAGACTT TGGGCACACA CAGCAGAGAG TCCGCTCTGC TGAATGGGAA TGGAAGGGAC 
GACTGCAAAG GAGCTCTGAC CCAGGCCTTG GAGATGAGAG AGAACTTCCA GAAACAACTA 
CAAGAGATCA TGTCAGCAAC CTCCATCGTG AGGACACCAG CCCAGAAGAC TTGCATGTCA 
AGAAGTAGTT GTAACAGCGC CTCTTCAACT CCTGACAGCA CTCGCTCTGC TCGGAGTGGA 
CGAAGCCGAG TCTTGGAGGC GCTCAGGCAG TCTCGACAGA TAGTGCCTGA TATCAACACA 
GAGCCCTCCC AGAATGAACA GATCATTTGG GATGACCCAA CAGCAAGGGA GGAGAGAGCA 
AGGCTTGCCA GCAACCTACA GTGGCCTAGC TACCCAACAC AGTATTCCGA GCTTCAGGTT 
GAGATAAAAA AACTGGATGA TTCTCCTTCT CGAAAGCCTG TGTACCATTC AGAAATTGCT 
GGGCAGGCCT CGTGTGTGAC TCAAGCCCCA GGGCAGCACC CAGGCTCTGA AGAACCAGAA 
GCTCCAGCAG CAGAAAGGAA TCTGATCCCT GAGCCTCAAG CCCCCAGCGT GGCTTTCCCC 
CTGGCTAAGC CGCCTGTAGC TCCACAACCT GAGGACAAGA TTGAAACACT GGAGGAGACG 
CATGGAAAAC TTAAAAAACA GTATGTGTTC CAGATGTCAT CTCTGAATTC TCAAGAGCGG 
ATTGATTACT GTCGCCTGAT TAAAGACCTA GGTGGGTCGG TGATCGAGAA GCAGTGCTCA 
GACCCCAGCT GCACACACAT GGTTGTGGGG TACCCACTGC GCAATGAGAA ATACTTAGCT 
TCCATGGCAG CTGGCAAATG GGTGCTTCAT CGCTCCTACC TGGACGCTTG CCGGACAGCT 
GGGCGCTTTG TGCAGGAGGA AGACTACGAG TGGGGAAGCA GCTCCATCCT AGATGCTCTC 
CCTGACGTCA CTGAGCACCA GCAGAAGCTC GCACTTGCAG CAATGAGATG GAGGAAAAGA 
ATCCAGCAGA GTCGGGAGTC GGGCATCGCT GAGGGAGCAT TTAGTGGCTG GAAAGTTATT 
TTGCGCGTTG ATCGGCCCCG AGAAGCAGGG TTTAAGCGCC TTCTTCAGGC AGGAGGAGCA 
AAGGTATTGC CTGGTCATCC AGAGCCTTTA TTTAAGGATG CTACACATCT CTTCTGTGAT 
TTCAATAAAC TGAAGCCTGA TGACTGCAGA GTTTTTATCG CAGAGGCTAC GGCCCAGAAC 
ATGGTCTGCT TGAAAACAGA ATATATTGCT GATTACCTCA TGATGGAATC CCCTCCTTGT 
GCTGATAATT ACCGAGTATC CGAAACTGCT TTATTCCGTA ACAAGGAAGG TGGGCCTGGA 
CTACCACAGA AGAGGAAAAC TCCTGCAGAG AACGTCATCA AACGGCCTCG GGTGCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_236578.7
CDS	205..4764
Translation	MSRNDQEPFFVKFLKSSDNSECFFKALESIKELQSEEYLQVITDEEALRIKENDKSLYICDRFSGTAFDHLKQLGCRIVGPQVVTFCMRHQQCVPRAEHPVYNMIMSDITISCTSLDKDKREEVHKYVQMMGGRVYRDLNVSVTHLIAGEVGSKKYLVAANLKKPILLPAWIKTLWEKSQEKKITKYTDVNMEDFKCPIFLGCIICVTGLNGIHRKTVQQLTAKHGGQYMGQLKMNECTHLIVQEPKGQKYECARRWNVHCVTLQWFHDSIEKGFCQDESIYKAEPRAAVKTVPDTSTPTAQSGSAAAENHRLADVSHISNINGSCVNETMFGSTTSKLECTLENLENLDISMFQAPEDLLDGCRIYLCGFSGRKLDKLRRLINSGGGVRFNQLNEDVTHVIMGDYEDDVRQFWSKSSHRPHVVGAKWLLECFSKGYILPEESYIHANYQPDGIPVSDQPRNQTTVLEKSGTFSKKDFVPNEKFQQTDEDLLAQYVHDDSTIVEAKLSEAIEPEVGPCGGSAHVEPCDDSTHISVQEENKSSVSHCLLDGSTVPEEGLFSQKSFLVLGFSVENECNIVNIIREHAGKIVSLPSRIVADYAVVPLLGCEVEATVAEVVTNTWLVTCIDNQTLVDPKSNPLFTPVSVMSGMTPLEDCVISFSQCVGAERDSLVFLANHLGASVQEFFVRKSNAKKGMLASTHLIVKEPTGSKYEAAKKWSLPAVNISWLLETARMGKRADENHFLVDSAPKQEQVLETNIPNGVSPNPDSPAHPDAHLEIHRQKAVTPLDMNRFQSKAFHAVISQHNRQGLASPPVRQPPKKEPSFHLDTPSKFLSKDKLFKPSFDVTDALAALETPNAASQKRKLSSPLSEVIVRNLTTALANSSRNTGCLSASPQLKGAHLEKEETPKPLDKVVVCVSKRLSKKQSELNGVAASLGAEYRWSFDETVTHYIYQGRANDSNREYKSAKERGVHVVSEHWLLECAQEYKHLPESLYPHTYNPKMSLDISTVQDGRLCDSRLPSALSASKDDEPDHLSVEENATETLGTHSRESALLNGNGRDDCKGALTQALEMRENFQKQLQEIMSATSIVRTPAQKTCMSRSSCNSASSTPDSTRSARSGRSRVLEALRQSRQIVPDINTEPSQNEQIIWDDPTAREERARLASNLQWPSYPTQYSELQVEIKKLDDSPSRKPVYHSEIAGQASCVTQAPGQHPGSEEPEAPAAERNLIPEPQAPSVAFPLAKPPVAPQPEDKIETLEETHGKLKKQYVFQMSSLNSQERIDYCRLIKDLGGSVIEKQCSDPSCTHMVVGYPLRNEKYLASMAAGKWVLHRSYLDACRTAGRFVQEEDYEWGSSSILDALPDVTEHQQKLALAAMRWRKRIQQSRESGIAEGAFSGWKVILRVDRPREAGFKRLLQAGGAKVLPGHPEPLFKDATHLFCDFNKLKPDDCRVFIAEATAQNMVCLKTEYIADYLMMESPPCADNYRVSETALFRNKEGGPGLPQKRKTPAENVIKRPRVH

Target ORF information:

RefSeq Version	XM_236578.8
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus topoisomerase (DNA) II binding protein 1 (Topbp1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_236578.8

ORF Insert Sequence:

ATGTCCAGAA ATGACCAAGA GCCGTTTTTT GTGAAGTTTT TAAAGTCTTC GGACAATTCA 
GAATGTTTTT TTAAAGCTCT TGAGTCAATA AAGGAGTTAC AATCAGAAGA GTATCTTCAG 
GTTATTACCG ATGAGGAAGC ATTGAGGATT AAAGAGAATG ACAAATCGCT TTATATCTGT 
GACCGTTTCA GTGGCACTGC TTTTGATCAC CTTAAACAGC TTGGCTGCAG AATTGTTGGT 
CCTCAAGTAG TCACATTTTG TATGCGCCAC CAGCAGTGTG TCCCAAGAGC TGAACATCCA 
GTTTATAATA TGATTATGTC TGACATAACT ATATCTTGTA CAAGCCTGGA TAAAGACAAG 
AGGGAAGAAG TTCATAAATA CGTACAAATG ATGGGTGGAC GAGTATACAG AGACCTTAAT 
GTCTCAGTCA CTCACCTTAT TGCAGGAGAA GTTGGGAGCA AAAAATACTT AGTTGCTGCT 
AATCTGAAGA AACCTATTTT GCTTCCTGCT TGGATTAAAA CACTTTGGGA GAAGTCACAA 
GAAAAAAAAA TAACCAAATA CACTGATGTA AACATGGAAG ACTTTAAGTG TCCTATTTTC 
CTTGGTTGCA TTATCTGTGT GACTGGCTTA AATGGTATAC ACAGGAAGAC AGTCCAGCAG 
CTCACAGCTA AGCATGGAGG CCAGTACATG GGGCAGCTGA AGATGAACGA GTGCACACAC 
CTCATTGTGC AAGAACCAAA AGGACAAAAA TATGAATGTG CCAGGAGATG GAATGTGCAC 
TGTGTGACCC TGCAATGGTT CCATGACAGT ATTGAGAAAG GCTTTTGTCA GGATGAGTCT 
ATATACAAGG CCGAACCCAG AGCAGCGGTA AAGACGGTGC CTGATACTTC CACACCCACT 
GCTCAGAGCG GCAGTGCTGC AGCTGAGAAT CACAGGCTGG CAGATGTCAG CCATATATCC 
AACATCAACG GCAGCTGTGT AAATGAAACA ATGTTTGGTT CAACTACGAG CAAACTCGAA 
TGCACACTTG AGAACCTAGA AAATCTGGAC ATCAGTATGT TTCAAGCACC TGAAGATCTA 
TTAGATGGCT GTCGGATATA TCTTTGTGGC TTTAGTGGCA GAAAGCTCGA TAAGTTGAGA 
AGGCTTATTA ACAGTGGAGG TGGAGTTCGG TTTAATCAAC TCAATGAAGA TGTAACTCAT 
GTAATCATGG GAGATTATGA GGATGACGTG AGGCAGTTTT GGAGCAAATC CTCCCACAGG 
CCCCATGTAG TGGGAGCAAA GTGGTTGCTG GAGTGTTTTA GTAAAGGATA TATACTTCCT 
GAAGAGTCAT ATATCCACGC TAACTACCAG CCAGATGGAA TTCCAGTTTC AGATCAGCCC 
AGAAACCAGA CAACTGTCTT AGAAAAGAGT GGCACATTCT CTAAGAAGGA CTTTGTTCCT 
AATGAGAAGT TTCAGCAGAC TGATGAGGAT CTGCTTGCTC AGTATGTACA TGATGACTCC 
ACAATAGTTG AAGCTAAGCT ATCTGAAGCT ATAGAGCCTG AAGTTGGGCC TTGCGGTGGT 
TCTGCTCATG TTGAGCCCTG TGATGATTCC ACTCACATTT CTGTGCAAGA GGAAAACAAG 
TCGTCTGTCA GTCATTGCCT CCTTGATGGC TCTACAGTTC CTGAGGAAGG CTTATTTAGC 
CAAAAGAGTT TCCTTGTTTT GGGTTTTAGT GTTGAAAATG AATGTAATAT TGTAAACATC 
ATAAGAGAGC ATGCTGGAAA GATTGTGTCC CTTCCCAGCA GAATTGTTGC TGACTATGCC 
GTGGTTCCCC TGCTGGGATG TGAAGTAGAA GCGACAGTGG CAGAAGTTGT TACAAACACA 
TGGCTGGTTA CATGCATAGA CAACCAGACA TTAGTTGATC CCAAGTCGAA TCCCCTCTTC 
ACGCCAGTCT CAGTAATGTC AGGAATGACT CCTTTAGAGG ACTGTGTGAT TTCCTTCAGT 
CAGTGTGTGG GAGCAGAAAG AGATTCCTTG GTATTCTTAG CAAATCATCT TGGAGCAAGT 
GTTCAAGAAT TCTTTGTCCG CAAATCCAAT GCAAAGAAAG GCATGCTCGC CAGTACACAT 
CTTATAGTGA AAGAACCGAC TGGTTCTAAA TATGAAGCTG CAAAGAAATG GAGTTTGCCA 
GCAGTTAATA TTTCGTGGCT CTTAGAAACT GCTAGAATGG GGAAGAGAGC AGATGAAAAC 
CATTTTCTGG TTGACAGTGC ACCTAAACAA GAACAAGTAT TAGAAACTAA CATACCAAAT 
GGAGTGAGTC CAAATCCAGA TTCTCCAGCA CATCCTGATG CACACTTGGA AATTCACAGA 
CAAAAGGCCG TTACACCTTT GGACATGAAC CGATTTCAGA GTAAAGCTTT CCATGCTGTC 
ATCTCACAGC ACAACAGGCA GGGCTTGGCC TCCCCACCAG TGAGACAGCC ACCTAAGAAG 
GAACCCTCCT TCCACCTGGA CACACCCTCA AAGTTCCTGT CCAAGGACAA GCTTTTCAAG 
CCATCCTTTG ATGTGACGGA TGCACTTGCA GCCTTAGAAA CCCCAAATGC TGCCAGCCAG 
AAGCGGAAAC TGAGCTCACC ACTCTCTGAA GTCATCGTCA GAAACCTGAC AACTGCTTTG 
GCAAATAGTT CACGAAACAC CGGCTGTCTT TCTGCTAGCC CTCAGCTAAA GGGTGCCCAT 
TTGGAGAAGG AAGAGACTCC GAAACCGCTT GACAAAGTTG TGGTGTGCGT GAGTAAAAGG 
CTCAGTAAGA AGCAGAGTGA GCTAAATGGG GTTGCAGCCT CACTGGGAGC AGAGTACAGG 
TGGAGTTTTG ATGAGACAGT TACTCATTAC ATTTACCAGG GTCGGGCAAA TGATAGTAAT 
CGCGAGTATA AATCTGCAAA GGAAAGAGGG GTGCACGTCG TGTCGGAGCA TTGGCTGTTA 
GAGTGTGCTC AAGAGTATAA GCATCTTCCA GAATCTCTTT ACCCACACAC TTACAACCCC 
AAAATGAGCT TGGACATCAG CACAGTGCAG GACGGGCGGC TCTGTGACAG CCGCTTACCC 
TCGGCACTTT CTGCATCTAA GGACGATGAG CCAGATCACC TGTCTGTAGA AGAAAATGCA 
ACAGAGACTT TGGGCACACA CAGCAGAGAG TCCGCTCTGC TGAATGGGAA TGGAAGGGAC 
GACTGCAAAG GAGCTCTGAC CCAGGCCTTG GAGATGAGAG AGAACTTCCA GAAACAACTA 
CAAGAGATCA TGTCAGCAAC CTCCATCGTG AGGACACCAG CCCAGAAGAC TTGCATGTCA 
AGAAGTAGTT GTAACAGCGC CTCTTCAACT CCTGACAGCA CTCGCTCTGC TCGGAGTGGA 
CGAAGCCGAG TCTTGGAGGC GCTCAGGCAG TCTCGACAGA TAGTGCCTGA TATCAACACA 
GAGCCCTCCC AGAATGAACA GATCATTTGG GATGACCCAA CAGCAAGGGA GGAGAGAGCA 
AGGCTTGCCA GCAACCTACA GTGGCCTAGC TACCCAACAC AGTATTCCGA GCTTCAGGTT 
GAGATAAAAA AACTGGATGA TTCTCCTTCT CGAAAGCCTG TGTACCATTC AGAAATTGCT 
GGGCAGGCCT CGTGTGTGAC TCAAGCCCCA GGGCAGCACC CAGGCTCTGA AGAACCAGAA 
GCTCCAGCAG CAGAAAGGAA TCTGATCCCT GAGCCTCAAG CCCCCAGCGT GGCTTTCCCC 
CTGGCTAAGC CGCCTGTAGC TCCACAACCT GAGGACAAGA TTGAAACACT GGAGGAGACG 
CATGGAAAAC TTAAAAAACA GTATGTGTTC CAGATGTCAT CTCTGAATTC TCAAGAGCGG 
ATTGATTACT GTCGCCTGAT TAAAGACCTA GGTGGGTCGG TGATCGAGAA GCAGTGCTCA 
GACCCCAGCT GCACACACAT GGTTGTGGGG TACCCACTGC GCAATGAGAA ATACTTAGCT 
TCCATGGCAG CTGGCAAATG GGTGCTTCAT CGCTCCTACC TGGACGCTTG CCGGACAGCT 
GGGCGCTTTG TGCAGGAGGA AGACTACGAG TGGGGAAGCA GCTCCATCCT AGATGCTCTC 
CCTGACGTCA CTGAGCACCA GCAGAAGCTC GCACTTGCAG CAATGAGATG GAGGAAAAGA 
ATCCAGCAGA GTCGGGAGTC GGGCATCGCT GAGGGAGCAT TTAGTGGCTG GAAAGTTATT 
TTGCGCGTTG ATCGGCCCCG AGAAGCAGGG TTTAAGCGCC TTCTTCAGGC AGGAGGAGCA 
AAGGTATTGC CTGGTCATCC AGAGCCTTTA TTTAAGGATG CTACACATCT CTTCTGTGAT 
TTCAATAAAC TGAAGCCTGA TGACTGCAGA GTTTTTATCG CAGAGGCTAC GGCCCAGAAC 
ATGGTCTGCT TGAAAACAGA ATATATTGCT GATTACCTCA TGATGGAATC CCCTCCTTGT 
GCTGATAATT ACCGAGTATC CGAAACTGCT TTATTCCGTA ACAAGGAAGG TGGGCCTGGA 
CTACCACAGA AGAGGAAAAC TCCTGCAGAG AACGTCATCA AACGGCCTCG GGTGCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Topbp1 ( XM_001072207.5 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_001072207.4 Rattus norvegicus topoisomerase (DNA) II binding protein 1 (Topbp1), transcript variant X4, mRNA.

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CloneID	ORa47491
Clone ID Related Accession (Same CDS sequence)	XM_236578.8 , XM_001072207.5 , XM_008757855.2
Accession Version	XM_001072207.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4560bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	DNA topoisomerase 2-binding protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (AC_000076.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_001072207.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAGAA ATGACCAAGA GCCGTTTTTT GTGAAGTTTT TAAAGTCTTC GGACAATTCA 
GAATGTTTTT TTAAAGCTCT TGAGTCAATA AAGGAGTTAC AATCAGAAGA GTATCTTCAG 
GTTATTACCG ATGAGGAAGC ATTGAGGATT AAAGAGAATG ACAAATCGCT TTATATCTGT 
GACCGTTTCA GTGGCACTGC TTTTGATCAC CTTAAACAGC TTGGCTGCAG AATTGTTGGT 
CCTCAAGTAG TCACATTTTG TATGCGCCAC CAGCAGTGTG TCCCAAGAGC TGAACATCCA 
GTTTATAATA TGATTATGTC TGACATAACT ATATCTTGTA CAAGCCTGGA TAAAGACAAG 
AGGGAAGAAG TTCATAAATA CGTACAAATG ATGGGTGGAC GAGTATACAG AGACCTTAAT 
GTCTCAGTCA CTCACCTTAT TGCAGGAGAA GTTGGGAGCA AAAAATACTT AGTTGCTGCT 
AATCTGAAGA AACCTATTTT GCTTCCTGCT TGGATTAAAA CACTTTGGGA GAAGTCACAA 
GAAAAAAAAA TAACCAAATA CACTGATGTA AACATGGAAG ACTTTAAGTG TCCTATTTTC 
CTTGGTTGCA TTATCTGTGT GACTGGCTTA AATGGTATAC ACAGGAAGAC AGTCCAGCAG 
CTCACAGCTA AGCATGGAGG CCAGTACATG GGGCAGCTGA AGATGAACGA GTGCACACAC 
CTCATTGTGC AAGAACCAAA AGGACAAAAA TATGAATGTG CCAGGAGATG GAATGTGCAC 
TGTGTGACCC TGCAATGGTT CCATGACAGT ATTGAGAAAG GCTTTTGTCA GGATGAGTCT 
ATATACAAGG CCGAACCCAG AGCAGCGGTA AAGACGGTGC CTGATACTTC CACACCCACT 
GCTCAGAGCG GCAGTGCTGC AGCTGAGAAT CACAGGCTGG CAGATGTCAG CCATATATCC 
AACATCAACG GCAGCTGTGT AAATGAAACA ATGTTTGGTT CAACTACGAG CAAACTCGAA 
TGCACACTTG AGAACCTAGA AAATCTGGAC ATCAGTATGT TTCAAGCACC TGAAGATCTA 
TTAGATGGCT GTCGGATATA TCTTTGTGGC TTTAGTGGCA GAAAGCTCGA TAAGTTGAGA 
AGGCTTATTA ACAGTGGAGG TGGAGTTCGG TTTAATCAAC TCAATGAAGA TGTAACTCAT 
GTAATCATGG GAGATTATGA GGATGACGTG AGGCAGTTTT GGAGCAAATC CTCCCACAGG 
CCCCATGTAG TGGGAGCAAA GTGGTTGCTG GAGTGTTTTA GTAAAGGATA TATACTTCCT 
GAAGAGTCAT ATATCCACGC TAACTACCAG CCAGATGGAA TTCCAGTTTC AGATCAGCCC 
AGAAACCAGA CAACTGTCTT AGAAAAGAGT GGCACATTCT CTAAGAAGGA CTTTGTTCCT 
AATGAGAAGT TTCAGCAGAC TGATGAGGAT CTGCTTGCTC AGTATGTACA TGATGACTCC 
ACAATAGTTG AAGCTAAGCT ATCTGAAGCT ATAGAGCCTG AAGTTGGGCC TTGCGGTGGT 
TCTGCTCATG TTGAGCCCTG TGATGATTCC ACTCACATTT CTGTGCAAGA GGAAAACAAG 
TCGTCTGTCA GTCATTGCCT CCTTGATGGC TCTACAGTTC CTGAGGAAGG CTTATTTAGC 
CAAAAGAGTT TCCTTGTTTT GGGTTTTAGT GTTGAAAATG AATGTAATAT TGTAAACATC 
ATAAGAGAGC ATGCTGGAAA GATTGTGTCC CTTCCCAGCA GAATTGTTGC TGACTATGCC 
GTGGTTCCCC TGCTGGGATG TGAAGTAGAA GCGACAGTGG CAGAAGTTGT TACAAACACA 
TGGCTGGTTA CATGCATAGA CAACCAGACA TTAGTTGATC CCAAGTCGAA TCCCCTCTTC 
ACGCCAGTCT CAGTAATGTC AGGAATGACT CCTTTAGAGG ACTGTGTGAT TTCCTTCAGT 
CAGTGTGTGG GAGCAGAAAG AGATTCCTTG GTATTCTTAG CAAATCATCT TGGAGCAAGT 
GTTCAAGAAT TCTTTGTCCG CAAATCCAAT GCAAAGAAAG GCATGCTCGC CAGTACACAT 
CTTATAGTGA AAGAACCGAC TGGTTCTAAA TATGAAGCTG CAAAGAAATG GAGTTTGCCA 
GCAGTTAATA TTTCGTGGCT CTTAGAAACT GCTAGAATGG GGAAGAGAGC AGATGAAAAC 
CATTTTCTGG TTGACAGTGC ACCTAAACAA GAACAAGTAT TAGAAACTAA CATACCAAAT 
GGAGTGAGTC CAAATCCAGA TTCTCCAGCA CATCCTGATG CACACTTGGA AATTCACAGA 
CAAAAGGCCG TTACACCTTT GGACATGAAC CGATTTCAGA GTAAAGCTTT CCATGCTGTC 
ATCTCACAGC ACAACAGGCA GGGCTTGGCC TCCCCACCAG TGAGACAGCC ACCTAAGAAG 
GAACCCTCCT TCCACCTGGA CACACCCTCA AAGTTCCTGT CCAAGGACAA GCTTTTCAAG 
CCATCCTTTG ATGTGACGGA TGCACTTGCA GCCTTAGAAA CCCCAAATGC TGCCAGCCAG 
AAGCGGAAAC TGAGCTCACC ACTCTCTGAA GTCATCGTCA GAAACCTGAC AACTGCTTTG 
GCAAATAGTT CACGAAACAC CGGCTGTCTT TCTGCTAGCC CTCAGCTAAA GGGTGCCCAT 
TTGGAGAAGG AAGAGACTCC GAAACCGCTT GACAAAGTTG TGGTGTGCGT GAGTAAAAGG 
CTCAGTAAGA AGCAGAGTGA GCTAAATGGG GTTGCAGCCT CACTGGGAGC AGAGTACAGG 
TGGAGTTTTG ATGAGACAGT TACTCATTAC ATTTACCAGG GTCGGGCAAA TGATAGTAAT 
CGCGAGTATA AATCTGCAAA GGAAAGAGGG GTGCACGTCG TGTCGGAGCA TTGGCTGTTA 
GAGTGTGCTC AAGAGTATAA GCATCTTCCA GAATCTCTTT ACCCACACAC TTACAACCCC 
AAAATGAGCT TGGACATCAG CACAGTGCAG GACGGGCGGC TCTGTGACAG CCGCTTACCC 
TCGGCACTTT CTGCATCTAA GGACGATGAG CCAGATCACC TGTCTGTAGA AGAAAATGCA 
ACAGAGACTT TGGGCACACA CAGCAGAGAG TCCGCTCTGC TGAATGGGAA TGGAAGGGAC 
GACTGCAAAG GAGCTCTGAC CCAGGCCTTG GAGATGAGAG AGAACTTCCA GAAACAACTA 
CAAGAGATCA TGTCAGCAAC CTCCATCGTG AGGACACCAG CCCAGAAGAC TTGCATGTCA 
AGAAGTAGTT GTAACAGCGC CTCTTCAACT CCTGACAGCA CTCGCTCTGC TCGGAGTGGA 
CGAAGCCGAG TCTTGGAGGC GCTCAGGCAG TCTCGACAGA TAGTGCCTGA TATCAACACA 
GAGCCCTCCC AGAATGAACA GATCATTTGG GATGACCCAA CAGCAAGGGA GGAGAGAGCA 
AGGCTTGCCA GCAACCTACA GTGGCCTAGC TACCCAACAC AGTATTCCGA GCTTCAGGTT 
GAGATAAAAA AACTGGATGA TTCTCCTTCT CGAAAGCCTG TGTACCATTC AGAAATTGCT 
GGGCAGGCCT CGTGTGTGAC TCAAGCCCCA GGGCAGCACC CAGGCTCTGA AGAACCAGAA 
GCTCCAGCAG CAGAAAGGAA TCTGATCCCT GAGCCTCAAG CCCCCAGCGT GGCTTTCCCC 
CTGGCTAAGC CGCCTGTAGC TCCACAACCT GAGGACAAGA TTGAAACACT GGAGGAGACG 
CATGGAAAAC TTAAAAAACA GTATGTGTTC CAGATGTCAT CTCTGAATTC TCAAGAGCGG 
ATTGATTACT GTCGCCTGAT TAAAGACCTA GGTGGGTCGG TGATCGAGAA GCAGTGCTCA 
GACCCCAGCT GCACACACAT GGTTGTGGGG TACCCACTGC GCAATGAGAA ATACTTAGCT 
TCCATGGCAG CTGGCAAATG GGTGCTTCAT CGCTCCTACC TGGACGCTTG CCGGACAGCT 
GGGCGCTTTG TGCAGGAGGA AGACTACGAG TGGGGAAGCA GCTCCATCCT AGATGCTCTC 
CCTGACGTCA CTGAGCACCA GCAGAAGCTC GCACTTGCAG CAATGAGATG GAGGAAAAGA 
ATCCAGCAGA GTCGGGAGTC GGGCATCGCT GAGGGAGCAT TTAGTGGCTG GAAAGTTATT 
TTGCGCGTTG ATCGGCCCCG AGAAGCAGGG TTTAAGCGCC TTCTTCAGGC AGGAGGAGCA 
AAGGTATTGC CTGGTCATCC AGAGCCTTTA TTTAAGGATG CTACACATCT CTTCTGTGAT 
TTCAATAAAC TGAAGCCTGA TGACTGCAGA GTTTTTATCG CAGAGGCTAC GGCCCAGAAC 
ATGGTCTGCT TGAAAACAGA ATATATTGCT GATTACCTCA TGATGGAATC CCCTCCTTGT 
GCTGATAATT ACCGAGTATC CGAAACTGCT TTATTCCGTA ACAAGGAAGG TGGGCCTGGA 
CTACCACAGA AGAGGAAAAC TCCTGCAGAG AACGTCATCA AACGGCCTCG GGTGCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001072207.4
CDS	205..4764
Translation	MSRNDQEPFFVKFLKSSDNSECFFKALESIKELQSEEYLQVITDEEALRIKENDKSLYICDRFSGTAFDHLKQLGCRIVGPQVVTFCMRHQQCVPRAEHPVYNMIMSDITISCTSLDKDKREEVHKYVQMMGGRVYRDLNVSVTHLIAGEVGSKKYLVAANLKKPILLPAWIKTLWEKSQEKKITKYTDVNMEDFKCPIFLGCIICVTGLNGIHRKTVQQLTAKHGGQYMGQLKMNECTHLIVQEPKGQKYECARRWNVHCVTLQWFHDSIEKGFCQDESIYKAEPRAAVKTVPDTSTPTAQSGSAAAENHRLADVSHISNINGSCVNETMFGSTTSKLECTLENLENLDISMFQAPEDLLDGCRIYLCGFSGRKLDKLRRLINSGGGVRFNQLNEDVTHVIMGDYEDDVRQFWSKSSHRPHVVGAKWLLECFSKGYILPEESYIHANYQPDGIPVSDQPRNQTTVLEKSGTFSKKDFVPNEKFQQTDEDLLAQYVHDDSTIVEAKLSEAIEPEVGPCGGSAHVEPCDDSTHISVQEENKSSVSHCLLDGSTVPEEGLFSQKSFLVLGFSVENECNIVNIIREHAGKIVSLPSRIVADYAVVPLLGCEVEATVAEVVTNTWLVTCIDNQTLVDPKSNPLFTPVSVMSGMTPLEDCVISFSQCVGAERDSLVFLANHLGASVQEFFVRKSNAKKGMLASTHLIVKEPTGSKYEAAKKWSLPAVNISWLLETARMGKRADENHFLVDSAPKQEQVLETNIPNGVSPNPDSPAHPDAHLEIHRQKAVTPLDMNRFQSKAFHAVISQHNRQGLASPPVRQPPKKEPSFHLDTPSKFLSKDKLFKPSFDVTDALAALETPNAASQKRKLSSPLSEVIVRNLTTALANSSRNTGCLSASPQLKGAHLEKEETPKPLDKVVVCVSKRLSKKQSELNGVAASLGAEYRWSFDETVTHYIYQGRANDSNREYKSAKERGVHVVSEHWLLECAQEYKHLPESLYPHTYNPKMSLDISTVQDGRLCDSRLPSALSASKDDEPDHLSVEENATETLGTHSRESALLNGNGRDDCKGALTQALEMRENFQKQLQEIMSATSIVRTPAQKTCMSRSSCNSASSTPDSTRSARSGRSRVLEALRQSRQIVPDINTEPSQNEQIIWDDPTAREERARLASNLQWPSYPTQYSELQVEIKKLDDSPSRKPVYHSEIAGQASCVTQAPGQHPGSEEPEAPAAERNLIPEPQAPSVAFPLAKPPVAPQPEDKIETLEETHGKLKKQYVFQMSSLNSQERIDYCRLIKDLGGSVIEKQCSDPSCTHMVVGYPLRNEKYLASMAAGKWVLHRSYLDACRTAGRFVQEEDYEWGSSSILDALPDVTEHQQKLALAAMRWRKRIQQSRESGIAEGAFSGWKVILRVDRPREAGFKRLLQAGGAKVLPGHPEPLFKDATHLFCDFNKLKPDDCRVFIAEATAQNMVCLKTEYIADYLMMESPPCADNYRVSETALFRNKEGGPGLPQKRKTPAENVIKRPRVH

Target ORF information:

RefSeq Version	XM_001072207.5
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus topoisomerase (DNA) II binding protein 1 (Topbp1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001072207.5

ORF Insert Sequence:

ATGTCCAGAA ATGACCAAGA GCCGTTTTTT GTGAAGTTTT TAAAGTCTTC GGACAATTCA 
GAATGTTTTT TTAAAGCTCT TGAGTCAATA AAGGAGTTAC AATCAGAAGA GTATCTTCAG 
GTTATTACCG ATGAGGAAGC ATTGAGGATT AAAGAGAATG ACAAATCGCT TTATATCTGT 
GACCGTTTCA GTGGCACTGC TTTTGATCAC CTTAAACAGC TTGGCTGCAG AATTGTTGGT 
CCTCAAGTAG TCACATTTTG TATGCGCCAC CAGCAGTGTG TCCCAAGAGC TGAACATCCA 
GTTTATAATA TGATTATGTC TGACATAACT ATATCTTGTA CAAGCCTGGA TAAAGACAAG 
AGGGAAGAAG TTCATAAATA CGTACAAATG ATGGGTGGAC GAGTATACAG AGACCTTAAT 
GTCTCAGTCA CTCACCTTAT TGCAGGAGAA GTTGGGAGCA AAAAATACTT AGTTGCTGCT 
AATCTGAAGA AACCTATTTT GCTTCCTGCT TGGATTAAAA CACTTTGGGA GAAGTCACAA 
GAAAAAAAAA TAACCAAATA CACTGATGTA AACATGGAAG ACTTTAAGTG TCCTATTTTC 
CTTGGTTGCA TTATCTGTGT GACTGGCTTA AATGGTATAC ACAGGAAGAC AGTCCAGCAG 
CTCACAGCTA AGCATGGAGG CCAGTACATG GGGCAGCTGA AGATGAACGA GTGCACACAC 
CTCATTGTGC AAGAACCAAA AGGACAAAAA TATGAATGTG CCAGGAGATG GAATGTGCAC 
TGTGTGACCC TGCAATGGTT CCATGACAGT ATTGAGAAAG GCTTTTGTCA GGATGAGTCT 
ATATACAAGG CCGAACCCAG AGCAGCGGTA AAGACGGTGC CTGATACTTC CACACCCACT 
GCTCAGAGCG GCAGTGCTGC AGCTGAGAAT CACAGGCTGG CAGATGTCAG CCATATATCC 
AACATCAACG GCAGCTGTGT AAATGAAACA ATGTTTGGTT CAACTACGAG CAAACTCGAA 
TGCACACTTG AGAACCTAGA AAATCTGGAC ATCAGTATGT TTCAAGCACC TGAAGATCTA 
TTAGATGGCT GTCGGATATA TCTTTGTGGC TTTAGTGGCA GAAAGCTCGA TAAGTTGAGA 
AGGCTTATTA ACAGTGGAGG TGGAGTTCGG TTTAATCAAC TCAATGAAGA TGTAACTCAT 
GTAATCATGG GAGATTATGA GGATGACGTG AGGCAGTTTT GGAGCAAATC CTCCCACAGG 
CCCCATGTAG TGGGAGCAAA GTGGTTGCTG GAGTGTTTTA GTAAAGGATA TATACTTCCT 
GAAGAGTCAT ATATCCACGC TAACTACCAG CCAGATGGAA TTCCAGTTTC AGATCAGCCC 
AGAAACCAGA CAACTGTCTT AGAAAAGAGT GGCACATTCT CTAAGAAGGA CTTTGTTCCT 
AATGAGAAGT TTCAGCAGAC TGATGAGGAT CTGCTTGCTC AGTATGTACA TGATGACTCC 
ACAATAGTTG AAGCTAAGCT ATCTGAAGCT ATAGAGCCTG AAGTTGGGCC TTGCGGTGGT 
TCTGCTCATG TTGAGCCCTG TGATGATTCC ACTCACATTT CTGTGCAAGA GGAAAACAAG 
TCGTCTGTCA GTCATTGCCT CCTTGATGGC TCTACAGTTC CTGAGGAAGG CTTATTTAGC 
CAAAAGAGTT TCCTTGTTTT GGGTTTTAGT GTTGAAAATG AATGTAATAT TGTAAACATC 
ATAAGAGAGC ATGCTGGAAA GATTGTGTCC CTTCCCAGCA GAATTGTTGC TGACTATGCC 
GTGGTTCCCC TGCTGGGATG TGAAGTAGAA GCGACAGTGG CAGAAGTTGT TACAAACACA 
TGGCTGGTTA CATGCATAGA CAACCAGACA TTAGTTGATC CCAAGTCGAA TCCCCTCTTC 
ACGCCAGTCT CAGTAATGTC AGGAATGACT CCTTTAGAGG ACTGTGTGAT TTCCTTCAGT 
CAGTGTGTGG GAGCAGAAAG AGATTCCTTG GTATTCTTAG CAAATCATCT TGGAGCAAGT 
GTTCAAGAAT TCTTTGTCCG CAAATCCAAT GCAAAGAAAG GCATGCTCGC CAGTACACAT 
CTTATAGTGA AAGAACCGAC TGGTTCTAAA TATGAAGCTG CAAAGAAATG GAGTTTGCCA 
GCAGTTAATA TTTCGTGGCT CTTAGAAACT GCTAGAATGG GGAAGAGAGC AGATGAAAAC 
CATTTTCTGG TTGACAGTGC ACCTAAACAA GAACAAGTAT TAGAAACTAA CATACCAAAT 
GGAGTGAGTC CAAATCCAGA TTCTCCAGCA CATCCTGATG CACACTTGGA AATTCACAGA 
CAAAAGGCCG TTACACCTTT GGACATGAAC CGATTTCAGA GTAAAGCTTT CCATGCTGTC 
ATCTCACAGC ACAACAGGCA GGGCTTGGCC TCCCCACCAG TGAGACAGCC ACCTAAGAAG 
GAACCCTCCT TCCACCTGGA CACACCCTCA AAGTTCCTGT CCAAGGACAA GCTTTTCAAG 
CCATCCTTTG ATGTGACGGA TGCACTTGCA GCCTTAGAAA CCCCAAATGC TGCCAGCCAG 
AAGCGGAAAC TGAGCTCACC ACTCTCTGAA GTCATCGTCA GAAACCTGAC AACTGCTTTG 
GCAAATAGTT CACGAAACAC CGGCTGTCTT TCTGCTAGCC CTCAGCTAAA GGGTGCCCAT 
TTGGAGAAGG AAGAGACTCC GAAACCGCTT GACAAAGTTG TGGTGTGCGT GAGTAAAAGG 
CTCAGTAAGA AGCAGAGTGA GCTAAATGGG GTTGCAGCCT CACTGGGAGC AGAGTACAGG 
TGGAGTTTTG ATGAGACAGT TACTCATTAC ATTTACCAGG GTCGGGCAAA TGATAGTAAT 
CGCGAGTATA AATCTGCAAA GGAAAGAGGG GTGCACGTCG TGTCGGAGCA TTGGCTGTTA 
GAGTGTGCTC AAGAGTATAA GCATCTTCCA GAATCTCTTT ACCCACACAC TTACAACCCC 
AAAATGAGCT TGGACATCAG CACAGTGCAG GACGGGCGGC TCTGTGACAG CCGCTTACCC 
TCGGCACTTT CTGCATCTAA GGACGATGAG CCAGATCACC TGTCTGTAGA AGAAAATGCA 
ACAGAGACTT TGGGCACACA CAGCAGAGAG TCCGCTCTGC TGAATGGGAA TGGAAGGGAC 
GACTGCAAAG GAGCTCTGAC CCAGGCCTTG GAGATGAGAG AGAACTTCCA GAAACAACTA 
CAAGAGATCA TGTCAGCAAC CTCCATCGTG AGGACACCAG CCCAGAAGAC TTGCATGTCA 
AGAAGTAGTT GTAACAGCGC CTCTTCAACT CCTGACAGCA CTCGCTCTGC TCGGAGTGGA 
CGAAGCCGAG TCTTGGAGGC GCTCAGGCAG TCTCGACAGA TAGTGCCTGA TATCAACACA 
GAGCCCTCCC AGAATGAACA GATCATTTGG GATGACCCAA CAGCAAGGGA GGAGAGAGCA 
AGGCTTGCCA GCAACCTACA GTGGCCTAGC TACCCAACAC AGTATTCCGA GCTTCAGGTT 
GAGATAAAAA AACTGGATGA TTCTCCTTCT CGAAAGCCTG TGTACCATTC AGAAATTGCT 
GGGCAGGCCT CGTGTGTGAC TCAAGCCCCA GGGCAGCACC CAGGCTCTGA AGAACCAGAA 
GCTCCAGCAG CAGAAAGGAA TCTGATCCCT GAGCCTCAAG CCCCCAGCGT GGCTTTCCCC 
CTGGCTAAGC CGCCTGTAGC TCCACAACCT GAGGACAAGA TTGAAACACT GGAGGAGACG 
CATGGAAAAC TTAAAAAACA GTATGTGTTC CAGATGTCAT CTCTGAATTC TCAAGAGCGG 
ATTGATTACT GTCGCCTGAT TAAAGACCTA GGTGGGTCGG TGATCGAGAA GCAGTGCTCA 
GACCCCAGCT GCACACACAT GGTTGTGGGG TACCCACTGC GCAATGAGAA ATACTTAGCT 
TCCATGGCAG CTGGCAAATG GGTGCTTCAT CGCTCCTACC TGGACGCTTG CCGGACAGCT 
GGGCGCTTTG TGCAGGAGGA AGACTACGAG TGGGGAAGCA GCTCCATCCT AGATGCTCTC 
CCTGACGTCA CTGAGCACCA GCAGAAGCTC GCACTTGCAG CAATGAGATG GAGGAAAAGA 
ATCCAGCAGA GTCGGGAGTC GGGCATCGCT GAGGGAGCAT TTAGTGGCTG GAAAGTTATT 
TTGCGCGTTG ATCGGCCCCG AGAAGCAGGG TTTAAGCGCC TTCTTCAGGC AGGAGGAGCA 
AAGGTATTGC CTGGTCATCC AGAGCCTTTA TTTAAGGATG CTACACATCT CTTCTGTGAT 
TTCAATAAAC TGAAGCCTGA TGACTGCAGA GTTTTTATCG CAGAGGCTAC GGCCCAGAAC 
ATGGTCTGCT TGAAAACAGA ATATATTGCT GATTACCTCA TGATGGAATC CCCTCCTTGT 
GCTGATAATT ACCGAGTATC CGAAACTGCT TTATTCCGTA ACAAGGAAGG TGGGCCTGGA 
CTACCACAGA AGAGGAAAAC TCCTGCAGAG AACGTCATCA AACGGCCTCG GGTGCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Topbp1 ( XM_008757855.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008756077.1 Rattus norvegicus topoisomerase (DNA) II binding protein 1 (Topbp1), transcript variant X5, mRNA.

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CloneID	ORa47491
Clone ID Related Accession (Same CDS sequence)	XM_236578.8 , XM_001072207.5 , XM_008757855.2
Accession Version	XM_008757855.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4560bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	DNA topoisomerase 2-binding protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (AC_000076.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008757855.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCAGAA ATGACCAAGA GCCGTTTTTT GTGAAGTTTT TAAAGTCTTC GGACAATTCA 
GAATGTTTTT TTAAAGCTCT TGAGTCAATA AAGGAGTTAC AATCAGAAGA GTATCTTCAG 
GTTATTACCG ATGAGGAAGC ATTGAGGATT AAAGAGAATG ACAAATCGCT TTATATCTGT 
GACCGTTTCA GTGGCACTGC TTTTGATCAC CTTAAACAGC TTGGCTGCAG AATTGTTGGT 
CCTCAAGTAG TCACATTTTG TATGCGCCAC CAGCAGTGTG TCCCAAGAGC TGAACATCCA 
GTTTATAATA TGATTATGTC TGACATAACT ATATCTTGTA CAAGCCTGGA TAAAGACAAG 
AGGGAAGAAG TTCATAAATA CGTACAAATG ATGGGTGGAC GAGTATACAG AGACCTTAAT 
GTCTCAGTCA CTCACCTTAT TGCAGGAGAA GTTGGGAGCA AAAAATACTT AGTTGCTGCT 
AATCTGAAGA AACCTATTTT GCTTCCTGCT TGGATTAAAA CACTTTGGGA GAAGTCACAA 
GAAAAAAAAA TAACCAAATA CACTGATGTA AACATGGAAG ACTTTAAGTG TCCTATTTTC 
CTTGGTTGCA TTATCTGTGT GACTGGCTTA AATGGTATAC ACAGGAAGAC AGTCCAGCAG 
CTCACAGCTA AGCATGGAGG CCAGTACATG GGGCAGCTGA AGATGAACGA GTGCACACAC 
CTCATTGTGC AAGAACCAAA AGGACAAAAA TATGAATGTG CCAGGAGATG GAATGTGCAC 
TGTGTGACCC TGCAATGGTT CCATGACAGT ATTGAGAAAG GCTTTTGTCA GGATGAGTCT 
ATATACAAGG CCGAACCCAG AGCAGCGGTA AAGACGGTGC CTGATACTTC CACACCCACT 
GCTCAGAGCG GCAGTGCTGC AGCTGAGAAT CACAGGCTGG CAGATGTCAG CCATATATCC 
AACATCAACG GCAGCTGTGT AAATGAAACA ATGTTTGGTT CAACTACGAG CAAACTCGAA 
TGCACACTTG AGAACCTAGA AAATCTGGAC ATCAGTATGT TTCAAGCACC TGAAGATCTA 
TTAGATGGCT GTCGGATATA TCTTTGTGGC TTTAGTGGCA GAAAGCTCGA TAAGTTGAGA 
AGGCTTATTA ACAGTGGAGG TGGAGTTCGG TTTAATCAAC TCAATGAAGA TGTAACTCAT 
GTAATCATGG GAGATTATGA GGATGACGTG AGGCAGTTTT GGAGCAAATC CTCCCACAGG 
CCCCATGTAG TGGGAGCAAA GTGGTTGCTG GAGTGTTTTA GTAAAGGATA TATACTTCCT 
GAAGAGTCAT ATATCCACGC TAACTACCAG CCAGATGGAA TTCCAGTTTC AGATCAGCCC 
AGAAACCAGA CAACTGTCTT AGAAAAGAGT GGCACATTCT CTAAGAAGGA CTTTGTTCCT 
AATGAGAAGT TTCAGCAGAC TGATGAGGAT CTGCTTGCTC AGTATGTACA TGATGACTCC 
ACAATAGTTG AAGCTAAGCT ATCTGAAGCT ATAGAGCCTG AAGTTGGGCC TTGCGGTGGT 
TCTGCTCATG TTGAGCCCTG TGATGATTCC ACTCACATTT CTGTGCAAGA GGAAAACAAG 
TCGTCTGTCA GTCATTGCCT CCTTGATGGC TCTACAGTTC CTGAGGAAGG CTTATTTAGC 
CAAAAGAGTT TCCTTGTTTT GGGTTTTAGT GTTGAAAATG AATGTAATAT TGTAAACATC 
ATAAGAGAGC ATGCTGGAAA GATTGTGTCC CTTCCCAGCA GAATTGTTGC TGACTATGCC 
GTGGTTCCCC TGCTGGGATG TGAAGTAGAA GCGACAGTGG CAGAAGTTGT TACAAACACA 
TGGCTGGTTA CATGCATAGA CAACCAGACA TTAGTTGATC CCAAGTCGAA TCCCCTCTTC 
ACGCCAGTCT CAGTAATGTC AGGAATGACT CCTTTAGAGG ACTGTGTGAT TTCCTTCAGT 
CAGTGTGTGG GAGCAGAAAG AGATTCCTTG GTATTCTTAG CAAATCATCT TGGAGCAAGT 
GTTCAAGAAT TCTTTGTCCG CAAATCCAAT GCAAAGAAAG GCATGCTCGC CAGTACACAT 
CTTATAGTGA AAGAACCGAC TGGTTCTAAA TATGAAGCTG CAAAGAAATG GAGTTTGCCA 
GCAGTTAATA TTTCGTGGCT CTTAGAAACT GCTAGAATGG GGAAGAGAGC AGATGAAAAC 
CATTTTCTGG TTGACAGTGC ACCTAAACAA GAACAAGTAT TAGAAACTAA CATACCAAAT 
GGAGTGAGTC CAAATCCAGA TTCTCCAGCA CATCCTGATG CACACTTGGA AATTCACAGA 
CAAAAGGCCG TTACACCTTT GGACATGAAC CGATTTCAGA GTAAAGCTTT CCATGCTGTC 
ATCTCACAGC ACAACAGGCA GGGCTTGGCC TCCCCACCAG TGAGACAGCC ACCTAAGAAG 
GAACCCTCCT TCCACCTGGA CACACCCTCA AAGTTCCTGT CCAAGGACAA GCTTTTCAAG 
CCATCCTTTG ATGTGACGGA TGCACTTGCA GCCTTAGAAA CCCCAAATGC TGCCAGCCAG 
AAGCGGAAAC TGAGCTCACC ACTCTCTGAA GTCATCGTCA GAAACCTGAC AACTGCTTTG 
GCAAATAGTT CACGAAACAC CGGCTGTCTT TCTGCTAGCC CTCAGCTAAA GGGTGCCCAT 
TTGGAGAAGG AAGAGACTCC GAAACCGCTT GACAAAGTTG TGGTGTGCGT GAGTAAAAGG 
CTCAGTAAGA AGCAGAGTGA GCTAAATGGG GTTGCAGCCT CACTGGGAGC AGAGTACAGG 
TGGAGTTTTG ATGAGACAGT TACTCATTAC ATTTACCAGG GTCGGGCAAA TGATAGTAAT 
CGCGAGTATA AATCTGCAAA GGAAAGAGGG GTGCACGTCG TGTCGGAGCA TTGGCTGTTA 
GAGTGTGCTC AAGAGTATAA GCATCTTCCA GAATCTCTTT ACCCACACAC TTACAACCCC 
AAAATGAGCT TGGACATCAG CACAGTGCAG GACGGGCGGC TCTGTGACAG CCGCTTACCC 
TCGGCACTTT CTGCATCTAA GGACGATGAG CCAGATCACC TGTCTGTAGA AGAAAATGCA 
ACAGAGACTT TGGGCACACA CAGCAGAGAG TCCGCTCTGC TGAATGGGAA TGGAAGGGAC 
GACTGCAAAG GAGCTCTGAC CCAGGCCTTG GAGATGAGAG AGAACTTCCA GAAACAACTA 
CAAGAGATCA TGTCAGCAAC CTCCATCGTG AGGACACCAG CCCAGAAGAC TTGCATGTCA 
AGAAGTAGTT GTAACAGCGC CTCTTCAACT CCTGACAGCA CTCGCTCTGC TCGGAGTGGA 
CGAAGCCGAG TCTTGGAGGC GCTCAGGCAG TCTCGACAGA TAGTGCCTGA TATCAACACA 
GAGCCCTCCC AGAATGAACA GATCATTTGG GATGACCCAA CAGCAAGGGA GGAGAGAGCA 
AGGCTTGCCA GCAACCTACA GTGGCCTAGC TACCCAACAC AGTATTCCGA GCTTCAGGTT 
GAGATAAAAA AACTGGATGA TTCTCCTTCT CGAAAGCCTG TGTACCATTC AGAAATTGCT 
GGGCAGGCCT CGTGTGTGAC TCAAGCCCCA GGGCAGCACC CAGGCTCTGA AGAACCAGAA 
GCTCCAGCAG CAGAAAGGAA TCTGATCCCT GAGCCTCAAG CCCCCAGCGT GGCTTTCCCC 
CTGGCTAAGC CGCCTGTAGC TCCACAACCT GAGGACAAGA TTGAAACACT GGAGGAGACG 
CATGGAAAAC TTAAAAAACA GTATGTGTTC CAGATGTCAT CTCTGAATTC TCAAGAGCGG 
ATTGATTACT GTCGCCTGAT TAAAGACCTA GGTGGGTCGG TGATCGAGAA GCAGTGCTCA 
GACCCCAGCT GCACACACAT GGTTGTGGGG TACCCACTGC GCAATGAGAA ATACTTAGCT 
TCCATGGCAG CTGGCAAATG GGTGCTTCAT CGCTCCTACC TGGACGCTTG CCGGACAGCT 
GGGCGCTTTG TGCAGGAGGA AGACTACGAG TGGGGAAGCA GCTCCATCCT AGATGCTCTC 
CCTGACGTCA CTGAGCACCA GCAGAAGCTC GCACTTGCAG CAATGAGATG GAGGAAAAGA 
ATCCAGCAGA GTCGGGAGTC GGGCATCGCT GAGGGAGCAT TTAGTGGCTG GAAAGTTATT 
TTGCGCGTTG ATCGGCCCCG AGAAGCAGGG TTTAAGCGCC TTCTTCAGGC AGGAGGAGCA 
AAGGTATTGC CTGGTCATCC AGAGCCTTTA TTTAAGGATG CTACACATCT CTTCTGTGAT 
TTCAATAAAC TGAAGCCTGA TGACTGCAGA GTTTTTATCG CAGAGGCTAC GGCCCAGAAC 
ATGGTCTGCT TGAAAACAGA ATATATTGCT GATTACCTCA TGATGGAATC CCCTCCTTGT 
GCTGATAATT ACCGAGTATC CGAAACTGCT TTATTCCGTA ACAAGGAAGG TGGGCCTGGA 
CTACCACAGA AGAGGAAAAC TCCTGCAGAG AACGTCATCA AACGGCCTCG GGTGCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008756077.1
CDS	71..4630
Translation	MSRNDQEPFFVKFLKSSDNSECFFKALESIKELQSEEYLQVITDEEALRIKENDKSLYICDRFSGTAFDHLKQLGCRIVGPQVVTFCMRHQQCVPRAEHPVYNMIMSDITISCTSLDKDKREEVHKYVQMMGGRVYRDLNVSVTHLIAGEVGSKKYLVAANLKKPILLPAWIKTLWEKSQEKKITKYTDVNMEDFKCPIFLGCIICVTGLNGIHRKTVQQLTAKHGGQYMGQLKMNECTHLIVQEPKGQKYECARRWNVHCVTLQWFHDSIEKGFCQDESIYKAEPRAAVKTVPDTSTPTAQSGSAAAENHRLADVSHISNINGSCVNETMFGSTTSKLECTLENLENLDISMFQAPEDLLDGCRIYLCGFSGRKLDKLRRLINSGGGVRFNQLNEDVTHVIMGDYEDDVRQFWSKSSHRPHVVGAKWLLECFSKGYILPEESYIHANYQPDGIPVSDQPRNQTTVLEKSGTFSKKDFVPNEKFQQTDEDLLAQYVHDDSTIVEAKLSEAIEPEVGPCGGSAHVEPCDDSTHISVQEENKSSVSHCLLDGSTVPEEGLFSQKSFLVLGFSVENECNIVNIIREHAGKIVSLPSRIVADYAVVPLLGCEVEATVAEVVTNTWLVTCIDNQTLVDPKSNPLFTPVSVMSGMTPLEDCVISFSQCVGAERDSLVFLANHLGASVQEFFVRKSNAKKGMLASTHLIVKEPTGSKYEAAKKWSLPAVNISWLLETARMGKRADENHFLVDSAPKQEQVLETNIPNGVSPNPDSPAHPDAHLEIHRQKAVTPLDMNRFQSKAFHAVISQHNRQGLASPPVRQPPKKEPSFHLDTPSKFLSKDKLFKPSFDVTDALAALETPNAASQKRKLSSPLSEVIVRNLTTALANSSRNTGCLSASPQLKGAHLEKEETPKPLDKVVVCVSKRLSKKQSELNGVAASLGAEYRWSFDETVTHYIYQGRANDSNREYKSAKERGVHVVSEHWLLECAQEYKHLPESLYPHTYNPKMSLDISTVQDGRLCDSRLPSALSASKDDEPDHLSVEENATETLGTHSRESALLNGNGRDDCKGALTQALEMRENFQKQLQEIMSATSIVRTPAQKTCMSRSSCNSASSTPDSTRSARSGRSRVLEALRQSRQIVPDINTEPSQNEQIIWDDPTAREERARLASNLQWPSYPTQYSELQVEIKKLDDSPSRKPVYHSEIAGQASCVTQAPGQHPGSEEPEAPAAERNLIPEPQAPSVAFPLAKPPVAPQPEDKIETLEETHGKLKKQYVFQMSSLNSQERIDYCRLIKDLGGSVIEKQCSDPSCTHMVVGYPLRNEKYLASMAAGKWVLHRSYLDACRTAGRFVQEEDYEWGSSSILDALPDVTEHQQKLALAAMRWRKRIQQSRESGIAEGAFSGWKVILRVDRPREAGFKRLLQAGGAKVLPGHPEPLFKDATHLFCDFNKLKPDDCRVFIAEATAQNMVCLKTEYIADYLMMESPPCADNYRVSETALFRNKEGGPGLPQKRKTPAENVIKRPRVH

Target ORF information:

RefSeq Version	XM_008757855.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus topoisomerase (DNA) II binding protein 1 (Topbp1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008757855.2

ORF Insert Sequence:

ATGTCCAGAA ATGACCAAGA GCCGTTTTTT GTGAAGTTTT TAAAGTCTTC GGACAATTCA 
GAATGTTTTT TTAAAGCTCT TGAGTCAATA AAGGAGTTAC AATCAGAAGA GTATCTTCAG 
GTTATTACCG ATGAGGAAGC ATTGAGGATT AAAGAGAATG ACAAATCGCT TTATATCTGT 
GACCGTTTCA GTGGCACTGC TTTTGATCAC CTTAAACAGC TTGGCTGCAG AATTGTTGGT 
CCTCAAGTAG TCACATTTTG TATGCGCCAC CAGCAGTGTG TCCCAAGAGC TGAACATCCA 
GTTTATAATA TGATTATGTC TGACATAACT ATATCTTGTA CAAGCCTGGA TAAAGACAAG 
AGGGAAGAAG TTCATAAATA CGTACAAATG ATGGGTGGAC GAGTATACAG AGACCTTAAT 
GTCTCAGTCA CTCACCTTAT TGCAGGAGAA GTTGGGAGCA AAAAATACTT AGTTGCTGCT 
AATCTGAAGA AACCTATTTT GCTTCCTGCT TGGATTAAAA CACTTTGGGA GAAGTCACAA 
GAAAAAAAAA TAACCAAATA CACTGATGTA AACATGGAAG ACTTTAAGTG TCCTATTTTC 
CTTGGTTGCA TTATCTGTGT GACTGGCTTA AATGGTATAC ACAGGAAGAC AGTCCAGCAG 
CTCACAGCTA AGCATGGAGG CCAGTACATG GGGCAGCTGA AGATGAACGA GTGCACACAC 
CTCATTGTGC AAGAACCAAA AGGACAAAAA TATGAATGTG CCAGGAGATG GAATGTGCAC 
TGTGTGACCC TGCAATGGTT CCATGACAGT ATTGAGAAAG GCTTTTGTCA GGATGAGTCT 
ATATACAAGG CCGAACCCAG AGCAGCGGTA AAGACGGTGC CTGATACTTC CACACCCACT 
GCTCAGAGCG GCAGTGCTGC AGCTGAGAAT CACAGGCTGG CAGATGTCAG CCATATATCC 
AACATCAACG GCAGCTGTGT AAATGAAACA ATGTTTGGTT CAACTACGAG CAAACTCGAA 
TGCACACTTG AGAACCTAGA AAATCTGGAC ATCAGTATGT TTCAAGCACC TGAAGATCTA 
TTAGATGGCT GTCGGATATA TCTTTGTGGC TTTAGTGGCA GAAAGCTCGA TAAGTTGAGA 
AGGCTTATTA ACAGTGGAGG TGGAGTTCGG TTTAATCAAC TCAATGAAGA TGTAACTCAT 
GTAATCATGG GAGATTATGA GGATGACGTG AGGCAGTTTT GGAGCAAATC CTCCCACAGG 
CCCCATGTAG TGGGAGCAAA GTGGTTGCTG GAGTGTTTTA GTAAAGGATA TATACTTCCT 
GAAGAGTCAT ATATCCACGC TAACTACCAG CCAGATGGAA TTCCAGTTTC AGATCAGCCC 
AGAAACCAGA CAACTGTCTT AGAAAAGAGT GGCACATTCT CTAAGAAGGA CTTTGTTCCT 
AATGAGAAGT TTCAGCAGAC TGATGAGGAT CTGCTTGCTC AGTATGTACA TGATGACTCC 
ACAATAGTTG AAGCTAAGCT ATCTGAAGCT ATAGAGCCTG AAGTTGGGCC TTGCGGTGGT 
TCTGCTCATG TTGAGCCCTG TGATGATTCC ACTCACATTT CTGTGCAAGA GGAAAACAAG 
TCGTCTGTCA GTCATTGCCT CCTTGATGGC TCTACAGTTC CTGAGGAAGG CTTATTTAGC 
CAAAAGAGTT TCCTTGTTTT GGGTTTTAGT GTTGAAAATG AATGTAATAT TGTAAACATC 
ATAAGAGAGC ATGCTGGAAA GATTGTGTCC CTTCCCAGCA GAATTGTTGC TGACTATGCC 
GTGGTTCCCC TGCTGGGATG TGAAGTAGAA GCGACAGTGG CAGAAGTTGT TACAAACACA 
TGGCTGGTTA CATGCATAGA CAACCAGACA TTAGTTGATC CCAAGTCGAA TCCCCTCTTC 
ACGCCAGTCT CAGTAATGTC AGGAATGACT CCTTTAGAGG ACTGTGTGAT TTCCTTCAGT 
CAGTGTGTGG GAGCAGAAAG AGATTCCTTG GTATTCTTAG CAAATCATCT TGGAGCAAGT 
GTTCAAGAAT TCTTTGTCCG CAAATCCAAT GCAAAGAAAG GCATGCTCGC CAGTACACAT 
CTTATAGTGA AAGAACCGAC TGGTTCTAAA TATGAAGCTG CAAAGAAATG GAGTTTGCCA 
GCAGTTAATA TTTCGTGGCT CTTAGAAACT GCTAGAATGG GGAAGAGAGC AGATGAAAAC 
CATTTTCTGG TTGACAGTGC ACCTAAACAA GAACAAGTAT TAGAAACTAA CATACCAAAT 
GGAGTGAGTC CAAATCCAGA TTCTCCAGCA CATCCTGATG CACACTTGGA AATTCACAGA 
CAAAAGGCCG TTACACCTTT GGACATGAAC CGATTTCAGA GTAAAGCTTT CCATGCTGTC 
ATCTCACAGC ACAACAGGCA GGGCTTGGCC TCCCCACCAG TGAGACAGCC ACCTAAGAAG 
GAACCCTCCT TCCACCTGGA CACACCCTCA AAGTTCCTGT CCAAGGACAA GCTTTTCAAG 
CCATCCTTTG ATGTGACGGA TGCACTTGCA GCCTTAGAAA CCCCAAATGC TGCCAGCCAG 
AAGCGGAAAC TGAGCTCACC ACTCTCTGAA GTCATCGTCA GAAACCTGAC AACTGCTTTG 
GCAAATAGTT CACGAAACAC CGGCTGTCTT TCTGCTAGCC CTCAGCTAAA GGGTGCCCAT 
TTGGAGAAGG AAGAGACTCC GAAACCGCTT GACAAAGTTG TGGTGTGCGT GAGTAAAAGG 
CTCAGTAAGA AGCAGAGTGA GCTAAATGGG GTTGCAGCCT CACTGGGAGC AGAGTACAGG 
TGGAGTTTTG ATGAGACAGT TACTCATTAC ATTTACCAGG GTCGGGCAAA TGATAGTAAT 
CGCGAGTATA AATCTGCAAA GGAAAGAGGG GTGCACGTCG TGTCGGAGCA TTGGCTGTTA 
GAGTGTGCTC AAGAGTATAA GCATCTTCCA GAATCTCTTT ACCCACACAC TTACAACCCC 
AAAATGAGCT TGGACATCAG CACAGTGCAG GACGGGCGGC TCTGTGACAG CCGCTTACCC 
TCGGCACTTT CTGCATCTAA GGACGATGAG CCAGATCACC TGTCTGTAGA AGAAAATGCA 
ACAGAGACTT TGGGCACACA CAGCAGAGAG TCCGCTCTGC TGAATGGGAA TGGAAGGGAC 
GACTGCAAAG GAGCTCTGAC CCAGGCCTTG GAGATGAGAG AGAACTTCCA GAAACAACTA 
CAAGAGATCA TGTCAGCAAC CTCCATCGTG AGGACACCAG CCCAGAAGAC TTGCATGTCA 
AGAAGTAGTT GTAACAGCGC CTCTTCAACT CCTGACAGCA CTCGCTCTGC TCGGAGTGGA 
CGAAGCCGAG TCTTGGAGGC GCTCAGGCAG TCTCGACAGA TAGTGCCTGA TATCAACACA 
GAGCCCTCCC AGAATGAACA GATCATTTGG GATGACCCAA CAGCAAGGGA GGAGAGAGCA 
AGGCTTGCCA GCAACCTACA GTGGCCTAGC TACCCAACAC AGTATTCCGA GCTTCAGGTT 
GAGATAAAAA AACTGGATGA TTCTCCTTCT CGAAAGCCTG TGTACCATTC AGAAATTGCT 
GGGCAGGCCT CGTGTGTGAC TCAAGCCCCA GGGCAGCACC CAGGCTCTGA AGAACCAGAA 
GCTCCAGCAG CAGAAAGGAA TCTGATCCCT GAGCCTCAAG CCCCCAGCGT GGCTTTCCCC 
CTGGCTAAGC CGCCTGTAGC TCCACAACCT GAGGACAAGA TTGAAACACT GGAGGAGACG 
CATGGAAAAC TTAAAAAACA GTATGTGTTC CAGATGTCAT CTCTGAATTC TCAAGAGCGG 
ATTGATTACT GTCGCCTGAT TAAAGACCTA GGTGGGTCGG TGATCGAGAA GCAGTGCTCA 
GACCCCAGCT GCACACACAT GGTTGTGGGG TACCCACTGC GCAATGAGAA ATACTTAGCT 
TCCATGGCAG CTGGCAAATG GGTGCTTCAT CGCTCCTACC TGGACGCTTG CCGGACAGCT 
GGGCGCTTTG TGCAGGAGGA AGACTACGAG TGGGGAAGCA GCTCCATCCT AGATGCTCTC 
CCTGACGTCA CTGAGCACCA GCAGAAGCTC GCACTTGCAG CAATGAGATG GAGGAAAAGA 
ATCCAGCAGA GTCGGGAGTC GGGCATCGCT GAGGGAGCAT TTAGTGGCTG GAAAGTTATT 
TTGCGCGTTG ATCGGCCCCG AGAAGCAGGG TTTAAGCGCC TTCTTCAGGC AGGAGGAGCA 
AAGGTATTGC CTGGTCATCC AGAGCCTTTA TTTAAGGATG CTACACATCT CTTCTGTGAT 
TTCAATAAAC TGAAGCCTGA TGACTGCAGA GTTTTTATCG CAGAGGCTAC GGCCCAGAAC 
ATGGTCTGCT TGAAAACAGA ATATATTGCT GATTACCTCA TGATGGAATC CCCTCCTTGT 
GCTGATAATT ACCGAGTATC CGAAACTGCT TTATTCCGTA ACAAGGAAGG TGGGCCTGGA 
CTACCACAGA AGAGGAAAAC TCCTGCAGAG AACGTCATCA AACGGCCTCG GGTGCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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Topbp1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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Eメール

電話

FAX

オンラインフォーム

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