gRNAs
The following Stag1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Stag1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
| CloneID | ORa06829 | |
| Clone ID Related Accession (Same CDS sequence) | NM_001108179.1 | |
| Accession Version | NM_001108179.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 3369bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector |  User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2018-02-04 | |
| Organism | Rattus norvegicus(Norway rat) | |
| Product | cohesin subunit SA-1 | |
| Comment | Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CH473954.2. On or before Oct 4, 2007 this sequence version replaced XM_236609.4, XM_001067112.1. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN02642904 [ECO:0000348] ##Evidence-Data-END## | |
1 | ATGATTACTT CAGAGTTACC AGTGTTACAG GATTCAACTA ATGAAACTAC TGCCCATTCT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_001101649.1 |
| CDS | 296..3664 |
| Translation |
Target ORF information:
Target ORF information:
|
 NM_001108179.1 |
1 | ATGATTACTT CAGAGTTACC AGTGTTACAG GATTCAACTA ATGAAACTAC TGCCCATTCT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | ORa31498 | |
| Clone ID Related Accession (Same CDS sequence) | XM_006243632.3 , XM_006243631.2 , XM_008766528.2 | |
| Accession Version | XM_006243632.3 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 3777bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2016-07-25 | |
| Organism | Rattus norvegicus(Norway rat) | |
| Product | cohesin subunit SA-1 isoform X1 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005107.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006243632.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGATTACTT CAGAGTTACC AGTGTTACAG GATTCAACTA ATGAAACTAC TGCCCATTCT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_006243694.1 |
| CDS | 310..4086 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_006243632.3 |
1 | ATGATTACTT CAGAGTTACC AGTGTTACAG GATTCAACTA ATGAAACTAC TGCCCATTCT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | ORa31498 | |
| Clone ID Related Accession (Same CDS sequence) | XM_006243632.3 , XM_006243631.2 , XM_008766528.2 | |
| Accession Version | XM_006243631.2 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 3777bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2016-07-25 | |
| Organism | Rattus norvegicus(Norway rat) | |
| Product | cohesin subunit SA-1 isoform X1 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005107.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 7, 2014 this sequence version replaced XM_006243631.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGATTACTT CAGAGTTACC AGTGTTACAG GATTCAACTA ATGAAACTAC TGCCCATTCT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_006243693.1 |
| CDS | 291..4067 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_006243631.2 |
1 | ATGATTACTT CAGAGTTACC AGTGTTACAG GATTCAACTA ATGAAACTAC TGCCCATTCT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | ORa31498 | |
| Clone ID Related Accession (Same CDS sequence) | XM_006243632.3 , XM_006243631.2 , XM_008766528.2 | |
| Accession Version | XM_008766528.2 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 3777bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2016-07-25 | |
| Organism | Rattus norvegicus(Norway rat) | |
| Product | cohesin subunit SA-1 isoform X1 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005107.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008766528.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGATTACTT CAGAGTTACC AGTGTTACAG GATTCAACTA ATGAAACTAC TGCCCATTCT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_008764750.1 |
| CDS | 269..4045 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_008766528.2 |
1 | ATGATTACTT CAGAGTTACC AGTGTTACAG GATTCAACTA ATGAAACTAC TGCCCATTCT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
Reduction of Nipbl impairs cohesin loading locally and affects transcription but not cohesion-dependent functions in a mouse model of Cornelia de Lange Syndrome. |
Cohesin regulates tissue-specific expression by stabilizing highly occupied cis-regulatory modules. |
In vitro loading of human cohesin on DNA by the human Scc2-Scc4 loader complex. |
A unique role of cohesin-SA1 in gene regulation and development. |
Human Scc4 is required for cohesin binding to chromatin, sister-chromatid cohesion, and mitotic progression. |
