gRNAs
The following Cgnl1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Cgnl1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
| CloneID | ORa06800 | |
| Clone ID Related Accession (Same CDS sequence) | XM_008766287.2 , NM_001108164.1 , XM_006243355.2 , XM_008766286.2 | |
| Accession Version | NM_001108164.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 3879bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector |  User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2017-10-01 | |
| Organism | Rattus norvegicus(Norway rat) | |
| Product | cingulin-like protein 1 | |
| Comment | Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CH474041.1. On or before Oct 4, 2007 this sequence version replaced XM_236385.4, XM_001054062.1. | |
1 | ATGGAGCTGT ATTTTGGTGA ATATCAACAC GTGCAGCAGG AATACGGTGT CCACCTGAGA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_001101634.1 |
| CDS | 155..4033 |
| Translation |
Target ORF information:
Target ORF information:
|
 NM_001108164.1 |
1 | ATGGAGCTGT ATTTTGGTGA ATATCAACAC GTGCAGCAGG AATACGGTGT CCACCTGAGA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | ORa06800 | |
| Clone ID Related Accession (Same CDS sequence) | XM_008766287.2 , NM_001108164.1 , XM_006243355.2 , XM_008766286.2 | |
| Accession Version | XM_008766287.2 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 3879bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2016-07-25 | |
| Organism | Rattus norvegicus(Norway rat) | |
| Product | cingulin-like protein 1 isoform X1 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005107.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008766287.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGGAGCTGT ATTTTGGTGA ATATCAACAC GTGCAGCAGG AATACGGTGT CCACCTGAGA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_008764509.1 |
| CDS | 262..4140 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_008766287.2 |
1 | ATGGAGCTGT ATTTTGGTGA ATATCAACAC GTGCAGCAGG AATACGGTGT CCACCTGAGA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | ORa06800 | |
| Clone ID Related Accession (Same CDS sequence) | XM_008766287.2 , NM_001108164.1 , XM_006243355.2 , XM_008766286.2 | |
| Accession Version | XM_006243355.2 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 3879bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2016-07-25 | |
| Organism | Rattus norvegicus(Norway rat) | |
| Product | cingulin-like protein 1 isoform X1 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005107.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006243355.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGGAGCTGT ATTTTGGTGA ATATCAACAC GTGCAGCAGG AATACGGTGT CCACCTGAGA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_006243417.1 |
| CDS | 123..4001 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_006243355.2 |
1 | ATGGAGCTGT ATTTTGGTGA ATATCAACAC GTGCAGCAGG AATACGGTGT CCACCTGAGA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | ORa06800 | |
| Clone ID Related Accession (Same CDS sequence) | XM_008766287.2 , NM_001108164.1 , XM_006243355.2 , XM_008766286.2 | |
| Accession Version | XM_008766286.2 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 3879bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2016-07-25 | |
| Organism | Rattus norvegicus(Norway rat) | |
| Product | cingulin-like protein 1 isoform X1 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005107.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008766286.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGGAGCTGT ATTTTGGTGA ATATCAACAC GTGCAGCAGG AATACGGTGT CCACCTGAGA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_008764508.1 |
| CDS | 491..4369 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_008766286.2 |
1 | ATGGAGCTGT ATTTTGGTGA ATATCAACAC GTGCAGCAGG AATACGGTGT CCACCTGAGA |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
Cingulin is dispensable for epithelial barrier function and tight junction structure, and plays a role in the control of claudin-2 expression and response to duodenal mucosa injury. |
Epithelial junction formation requires confinement of Cdc42 activity by a novel SH3BP1 complex. |
A role for ZO-1 and PLEKHA7 in recruiting paracingulin to tight and adherens junctions of epithelial cells. |
JACOP, a novel plaque protein localizing at the apical junctional complex with sequence similarity to cingulin. |
