gRNAs
The following Espl1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Espl1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
| CloneID | ORa06375 | |
| Clone ID Related Accession (Same CDS sequence) | XM_006242460.3 , NM_001170602.1 | |
| Accession Version | NM_001170602.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 6330bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector |  User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-01-22 | |
| Organism | Rattus norvegicus(Norway rat) | |
| Product | separin | |
| Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC097309.7. On Dec 15, 2013 this sequence version replaced XM_006242463.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA2689604, SAMN00632251 [ECO:0000348] ##Evidence-Data-END## | |
1 | ATGAGGAACT TCAAAGGAGT CAAGTTCGCC ACTCTACTTT GCTGCAAGAG AGAGACCCAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_001164073.1 |
| CDS | 56..6385 |
| Translation |
Target ORF information:
Target ORF information:
|
 NM_001170602.1 |
1 | ATGAGGAACT TCAAAGGAGT CAAGTTCGCC ACTCTACTTT GCTGCAAGAG AGAGACCCAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | ORa06375 | |
| Clone ID Related Accession (Same CDS sequence) | XM_006242460.3 , NM_001170602.1 | |
| Accession Version | XM_006242460.3 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 6330bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2016-07-25 | |
| Organism | Rattus norvegicus(Norway rat) | |
| Product | separin isoform X1 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005106.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006242460.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGAGGAACT TCAAAGGAGT CAAGTTCGCC ACTCTACTTT GCTGCAAGAG AGAGACCCAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_006242522.1 |
| CDS | 209..6538 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_006242460.3 |
1 | ATGAGGAACT TCAAAGGAGT CAAGTTCGCC ACTCTACTTT GCTGCAAGAG AGAGACCCAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
Wapl is an essential regulator of chromatin structure and chromosome segregation. |
Role of cleavage by separase of the Rec8 kleisin subunit of cohesin during mammalian meiosis I. |
Separase: a universal trigger for sister chromatid disjunction but not chromosome cycle progression. |
The meiosis I-to-meiosis II transition in mouse oocytes requires separase activity. |
Processing, localization, and requirement of human separase for normal anaphase progression. |
