Prickle1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Prickle1
Entrez Gene ID	315259
Full Name	prickle planar cell polarity protein 1
Synonyms	Rilp
General protein information	Preferred Names prickle planar cell polarity protein 1 Names prickle-like protein 1 REST/NRSF-interacting LIM domain protein 1 prickle homolog 1 prickle-like 1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 7 Map Location: 7q35
Summary	plays a role in the nuclear localization of transcriptional regulators REST4 and REST/NRSF; may act as a nuclear receptor for transcription factors [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_006242213.3	XP_006242275.1	prickle-like protein 1 isoform X1
XM_006242214.3	XP_006242276.1	prickle-like protein 1 isoform X1
XM_017594887.1	XP_017450376.1	prickle-like protein 1 isoform X1
XM_006242215.3	XP_006242277.1	prickle-like protein 1 isoform X1
NM_199396.1	NP_955428.1	prickle-like protein 1

Pathways from BioSystems

Homologies

Bos taurus (cattle)	PRICKLE1	NP_001096004.1
Mus musculus (house mouse)	Prickle1	NP_001028389.1
Rattus norvegicus (Norway rat)	Prickle1	NP_955428.1
Xenopus tropicalis (tropical clawed frog)	prickle1	NP_001016939.1
Pan troglodytes (chimpanzee)	PRICKLE1	XP_001166831.1
Gallus gallus (chicken)	PRICKLE1	XP_416036.2
Homo sapiens (human)	PRICKLE1	NP_001138355.1
	PRICKLE1	XP_002798581.1
Canis lupus familiaris (dog)	PRICKLE1	XP_005637023.1
Danio rerio (zebrafish)	prickle1a	NP_899185.2

Gene Ontology
Bibliography

Related articles in PubMed

Global genetic analysis in mice unveils central role for cilia in congenital heart disease.
Li Y, Klena NT, Gabriel GC, Liu X, Kim AJ, Lemke K, Chen Y, Chatterjee B, Devine W, Damerla RR, Chang C, Yagi H, San Agustin JT, Thahir M, Anderton S, Lawhead C, Vescovi A, Pratt H, Morgan J, Haynes L, Smith CL, Eppig JT, Reinholdt L, Francis R, Leatherbury L, Ganapathiraju MK, Tobita K, Pazour GJ, Lo CW
Nature521(7553)520-4(2015 May)

PRICKLE1 interaction with SYNAPSIN I reveals a role in autism spectrum disorders.
Paemka L, Mahajan VB, Skeie JM, Sowers LP, Ehaideb SN, Gonzalez-Alegre P, Sasaoka T, Tao H, Miyagi A, Ueno N, Takao K, Miyakawa T, Wu S, Darbro BW, Ferguson PJ, Pieper AA, Britt JK, Wemmie JA, Rudd DS, Wassink T, El-Shanti H, Mefford HC, Carvill GL, Manak JR, Bassuk AG
PloS one8(12)e80737(2013)

Nuclear localization of Prickle2 is required to establish cell polarity during early mouse embryogenesis.
Tao H, Inoue K, Kiyonari H, Bassuk AG, Axelrod JD, Sasaki H, Aizawa S, Ueno N
Developmental biology364(2)138-48(2012 Apr)

Identification and characterization of novel rare mutations in the planar cell polarity gene PRICKLE1 in human neural tube defects.
Bosoi CM, Capra V, Allache R, Trinh VQ, De Marco P, Merello E, Drapeau P, Bassuk AG, Kibar Z
Human mutation32(12)1371-5(2011 Dec)

Neuron-restrictive silencer factor-mediated hyperpolarization-activated cyclic nucleotide gated channelopathy in experimental temporal lobe epilepsy.
McClelland S, Flynn C, Dub? C, Richichi C, Zha Q, Ghestem A, Esclapez M, Bernard C, Baram TZ
Annals of neurology70(3)454-64(2011 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

these findings suggest PRICKLE1 mutations contribute to ASD by disrupting the interaction with SYN1 and regulation of synaptic vesicles.
Title: PRICKLE1 interaction with SYNAPSIN I reveals a role in autism spectrum disorders.

We conclude that the NRSE-NRSF system was implicated in HCN4 expression in cardiac myocytes.
Title: NRSF regulates the developmental and hypertrophic changes of HCN4 transcription in rat cardiac myocytes.

appears to serve as a nuclear receptor for REST/NRSF, REST4, and possibly other transcription factors
Title: REST/NRSF-interacting LIM domain protein, a putative nuclear translocation receptor.

The following Prickle1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Prickle1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa05310	NM_199396.1 Latest version!	Rattus norvegicus prickle planar cell polarity protein 1 (Prickle1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
ORa05310	XM_006242213.3 Latest version!	Rattus norvegicus prickle planar cell polarity protein 1 (Prickle1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
ORa05310	XM_006242215.3 Latest version!	Rattus norvegicus prickle planar cell polarity protein 1 (Prickle1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
ORa05310	XM_017594887.1 Latest version!	Rattus norvegicus prickle planar cell polarity protein 1 (Prickle1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
ORa05310	XM_006242214.3 Latest version!	Rattus norvegicus prickle planar cell polarity protein 1 (Prickle1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Prickle1 ( NM_199396.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_955428.1 Rattus norvegicus prickle planar cell polarity protein 1 (Prickle1), mRNA.

Price & Availability↑

CloneID	ORa05310
Clone ID Related Accession (Same CDS sequence)	XM_006242213.3 , XM_006242214.3 , XM_017594887.1 , XM_006242215.3 , NM_199396.1
Accession Version	NM_199396.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2496bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Rattus norvegicus(Norway rat)
Product	prickle-like protein 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CH474027.1. On or before Oct 24, 2008 this sequence version replaced XM_001057488.1, XM_001057421.1, XM_235609.4, XM_001075319.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGCCTTTGG AGATGGAGCC CAAAATGAGC AAACTGGCCT TTGGGTGCCA GAGAAGTTCC 
ACATCAGACG ACGATTCCGG GTGCGCCCTG GAAGAGTATG CCTGGGTCCC ACCGGGCCTC 
AGGCCTGAGC AGATCCAGCT CTATTTCGCA TGCTTGCCAG AGGAAAAGGT TCCTTACGTT 
AACAGCCCTG GAGAGAAACA CCGGATTAAA CAGCTTTTGT ACCAGTTGCC ACCACACGAT 
AACGAGGTGA GGTACTGCCA GTCTTTAAGC GAAGAGGAGA AGAAGGAATT GCAGGTGTTC 
AGTGCTCAGA GGAAGAAAGA AGCTCTCGGA AGAGGAACCA TTAAACTCTT GTCCAGAGCA 
ATGATGCACG CAGTGTGTGA GCAGTGTGGG TTGCAGATGA ACGGAGGGGA GGTCGCAGTG 
TTTGCCTCCC GTGCGGGTCC TGGAGTGTGC TGGCATCCAT CCTGCTTTGT CTGCTTCACG 
TGTAATGAAC TACTGGTCGA CCTCATCTAT TTCTATCAGG ATGGAAAAAT CCACTGTGGC 
AGGCACCATG CCGAACTGCT CAAACCTCGG TGCTCAGCCT GTGACGAGAT CATTTTTGCG 
GACGAGTGCA CAGAGGCCGA GGGCCGCCAC TGGCACATGA AGCATTTCTG CTGCCTAGAA 
TGTGAAACGG TCCTGGGAGG GCAGAGATAT ATCATGAAGG ATGGCCGCCC CTTCTGCTGC 
GGCTGCTTCG AGTCTCTCTA CGCGGAGTAC TGCGAAACCT GTGGCGAGCA TATTGGTGTG 
GACCATGCCC AGATGACCTA TGACGGACAA CATTGGCATG CCACCGAGGC CTGCTTTTCC 
TGTGCTCAAT GTAAGGCCTC TTTGCTGGGA TGCCCCTTCC TCCCAAAACA AGGTCAGATC 
TACTGCTCCA AGACCTGCAG CCTTGGGGAA GACATCCACG CCTCCGATTC CTCTGACTCT 
GCCTTCCAGT CAGCCCGATC GAGAGACTCC CGGAGAAGTG TGAGGATGGG CAGAAGCAGC 
CGCTCTGCTG ACCAGTGCAG GCAGTCTCTC CTTCTGTCTC CCGCTCTGAA CTACAAGTTC 
CCGGGTCTTT CTGGCAGTGC TGACGACACT CTCTCTCGGA AGCTGGACGA CGTGAGCCTC 
TCCGGGCAGG GAGCGGGCTT TGCTCATGAG GAATTCTGGA AAGCCAGAGT GGATCAGGAA 
GCCTCTGAAG ACCCTGAAGA ATGGGCTGAG CACGAAGATT ATATGACACA GCTCCTCCTC 
AAGTTTGGTG ATAAAAACCT CTTCCAGCAG CCGCCCAGCG AGGTGGACAT GAGAGCCAGT 
GAGCACTGGA TACCCGACAA CATGGTTACG AATAAGCCGG AGGCAAAGCA GAATCACCAG 
AGCCTTGCGA GTAAAAAGTA TCAGTCGGAT ATGTACTGGG CCCAGTCCCA AGACGGGCTG 
GGTGACTCCG CCTACGGCAG CCACCCAGGC CCTGCCAGCA GCCGAAGGCT GCAAGAGCTA 
GATCTGGACC ACGGTGCTGC GGGGTATAAC CATGACCAAA CCCAGTGGTA TGAAGACTCC 
CTGGAGTGCC TGTCTGACTT GAAACCAGAG CAGAGTGTCC GGGATTCTAT GGATTCTCTG 
GCGTTGTCCA ACATCACAGG GGCATCCGTG GACGGAGAAA GCAAGCCAAG GCCATCATTA 
TACTCTCTCC AAAACTTCGA GGAGATAGAG GCAGAAGACT GTGAGAAAAT GAGCAATATG 
GGAACTCTGA ATTCTTCCAT GCTGCACAGG AGCGCAGAGT CCTTAAAAAG TCTGAATTCA 
GAACTGTGCC CAGAAAAAAT TATACCGGAG GAAAAACCAG TGCACCTGCC GGTGCTCAGA 
AGATCCAAGT CTCAGTCCCG ACCACAGCAG GTCAAGTTTT CAGATGACGT CATCGACAAC 
GGAAGCTACG ACATCGAAAT CCGGCAGCCT CCAATGAGTG AAAGGACTCG GAGACGAGTG 
TATCACTTTG AAGAGAGGGG ATCCAGGCCT CACCACCATC GCCACCGGAG AAGCAGAAAG 
TCGCGGTCCG ACAACGCCCT GAACCTGGTC ACAGAAAGGA AGTACTCCGC CAAGGACAGA 
CTTCGGCTGT ACACCCCCGA TAACTATGAG AAATTCATAC AGAGTAAGGG CGCCCGGGAG 
CTCCAAGCAT ACATGCAGAA TGCCAACCTC TACGGCCAGT ATGCCCACAC CACATCGGAT 
TATGCCCTGC AGAACCCAGG AATGACCAGG TTCCTGGGGC TCTACGGGGA CGACGATGAC 
TCCTGGTGTT CATCCTCCAC TTCCTCCTCC GACTCAGAAG AAGAAGGCTA TTTTCTTGGA 
CAGCCAATCC CTCAGCCTCG GCCACAAAGA TTCACCTACT ATACGGATGA CCTTTCTAGT 
CCGGCTTCTG CACTGCCCAC CCCACAGTTT AATCAGAGGA CAACTAAATC CAAGAAGAAA 
AAGGGACACA GGGGCAAAAA CTGTATCATT TCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_955428.1
CDS	629..3124
Translation	MPLEMEPKMSKLAFGCQRSSTSDDDSGCALEEYAWVPPGLRPEQIQLYFACLPEEKVPYVNSPGEKHRIKQLLYQLPPHDNEVRYCQSLSEEEKKELQVFSAQRKKEALGRGTIKLLSRAMMHAVCEQCGLQMNGGEVAVFASRAGPGVCWHPSCFVCFTCNELLVDLIYFYQDGKIHCGRHHAELLKPRCSACDEIIFADECTEAEGRHWHMKHFCCLECETVLGGQRYIMKDGRPFCCGCFESLYAEYCETCGEHIGVDHAQMTYDGQHWHATEACFSCAQCKASLLGCPFLPKQGQIYCSKTCSLGEDIHASDSSDSAFQSARSRDSRRSVRMGRSSRSADQCRQSLLLSPALNYKFPGLSGSADDTLSRKLDDVSLSGQGAGFAHEEFWKARVDQEASEDPEEWAEHEDYMTQLLLKFGDKNLFQQPPSEVDMRASEHWIPDNMVTNKPEAKQNHQSLASKKYQSDMYWAQSQDGLGDSAYGSHPGPASSRRLQELDLDHGAAGYNHDQTQWYEDSLECLSDLKPEQSVRDSMDSLALSNITGASVDGESKPRPSLYSLQNFEEIEAEDCEKMSNMGTLNSSMLHRSAESLKSLNSELCPEKIIPEEKPVHLPVLRRSKSQSRPQQVKFSDDVIDNGSYDIEIRQPPMSERTRRRVYHFEERGSRPHHHRHRRSRKSRSDNALNLVTERKYSAKDRLRLYTPDNYEKFIQSKGARELQAYMQNANLYGQYAHTTSDYALQNPGMTRFLGLYGDDDDSWCSSSTSSSDSEEEGYFLGQPIPQPRPQRFTYYTDDLSSPASALPTPQFNQRTTKSKKKKGHRGKNCIIS

Target ORF information:

RefSeq Version	NM_199396.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus prickle planar cell polarity protein 1 (Prickle1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_199396.1

ORF Insert Sequence:

ATGCCTTTGG AGATGGAGCC CAAAATGAGC AAACTGGCCT TTGGGTGCCA GAGAAGTTCC 
ACATCAGACG ACGATTCCGG GTGCGCCCTG GAAGAGTATG CCTGGGTCCC ACCGGGCCTC 
AGGCCTGAGC AGATCCAGCT CTATTTCGCA TGCTTGCCAG AGGAAAAGGT TCCTTACGTT 
AACAGCCCTG GAGAGAAACA CCGGATTAAA CAGCTTTTGT ACCAGTTGCC ACCACACGAT 
AACGAGGTGA GGTACTGCCA GTCTTTAAGC GAAGAGGAGA AGAAGGAATT GCAGGTGTTC 
AGTGCTCAGA GGAAGAAAGA AGCTCTCGGA AGAGGAACCA TTAAACTCTT GTCCAGAGCA 
ATGATGCACG CAGTGTGTGA GCAGTGTGGG TTGCAGATGA ACGGAGGGGA GGTCGCAGTG 
TTTGCCTCCC GTGCGGGTCC TGGAGTGTGC TGGCATCCAT CCTGCTTTGT CTGCTTCACG 
TGTAATGAAC TACTGGTCGA CCTCATCTAT TTCTATCAGG ATGGAAAAAT CCACTGTGGC 
AGGCACCATG CCGAACTGCT CAAACCTCGG TGCTCAGCCT GTGACGAGAT CATTTTTGCG 
GACGAGTGCA CAGAGGCCGA GGGCCGCCAC TGGCACATGA AGCATTTCTG CTGCCTAGAA 
TGTGAAACGG TCCTGGGAGG GCAGAGATAT ATCATGAAGG ATGGCCGCCC CTTCTGCTGC 
GGCTGCTTCG AGTCTCTCTA CGCGGAGTAC TGCGAAACCT GTGGCGAGCA TATTGGTGTG 
GACCATGCCC AGATGACCTA TGACGGACAA CATTGGCATG CCACCGAGGC CTGCTTTTCC 
TGTGCTCAAT GTAAGGCCTC TTTGCTGGGA TGCCCCTTCC TCCCAAAACA AGGTCAGATC 
TACTGCTCCA AGACCTGCAG CCTTGGGGAA GACATCCACG CCTCCGATTC CTCTGACTCT 
GCCTTCCAGT CAGCCCGATC GAGAGACTCC CGGAGAAGTG TGAGGATGGG CAGAAGCAGC 
CGCTCTGCTG ACCAGTGCAG GCAGTCTCTC CTTCTGTCTC CCGCTCTGAA CTACAAGTTC 
CCGGGTCTTT CTGGCAGTGC TGACGACACT CTCTCTCGGA AGCTGGACGA CGTGAGCCTC 
TCCGGGCAGG GAGCGGGCTT TGCTCATGAG GAATTCTGGA AAGCCAGAGT GGATCAGGAA 
GCCTCTGAAG ACCCTGAAGA ATGGGCTGAG CACGAAGATT ATATGACACA GCTCCTCCTC 
AAGTTTGGTG ATAAAAACCT CTTCCAGCAG CCGCCCAGCG AGGTGGACAT GAGAGCCAGT 
GAGCACTGGA TACCCGACAA CATGGTTACG AATAAGCCGG AGGCAAAGCA GAATCACCAG 
AGCCTTGCGA GTAAAAAGTA TCAGTCGGAT ATGTACTGGG CCCAGTCCCA AGACGGGCTG 
GGTGACTCCG CCTACGGCAG CCACCCAGGC CCTGCCAGCA GCCGAAGGCT GCAAGAGCTA 
GATCTGGACC ACGGTGCTGC GGGGTATAAC CATGACCAAA CCCAGTGGTA TGAAGACTCC 
CTGGAGTGCC TGTCTGACTT GAAACCAGAG CAGAGTGTCC GGGATTCTAT GGATTCTCTG 
GCGTTGTCCA ACATCACAGG GGCATCCGTG GACGGAGAAA GCAAGCCAAG GCCATCATTA 
TACTCTCTCC AAAACTTCGA GGAGATAGAG GCAGAAGACT GTGAGAAAAT GAGCAATATG 
GGAACTCTGA ATTCTTCCAT GCTGCACAGG AGCGCAGAGT CCTTAAAAAG TCTGAATTCA 
GAACTGTGCC CAGAAAAAAT TATACCGGAG GAAAAACCAG TGCACCTGCC GGTGCTCAGA 
AGATCCAAGT CTCAGTCCCG ACCACAGCAG GTCAAGTTTT CAGATGACGT CATCGACAAC 
GGAAGCTACG ACATCGAAAT CCGGCAGCCT CCAATGAGTG AAAGGACTCG GAGACGAGTG 
TATCACTTTG AAGAGAGGGG ATCCAGGCCT CACCACCATC GCCACCGGAG AAGCAGAAAG 
TCGCGGTCCG ACAACGCCCT GAACCTGGTC ACAGAAAGGA AGTACTCCGC CAAGGACAGA 
CTTCGGCTGT ACACCCCCGA TAACTATGAG AAATTCATAC AGAGTAAGGG CGCCCGGGAG 
CTCCAAGCAT ACATGCAGAA TGCCAACCTC TACGGCCAGT ATGCCCACAC CACATCGGAT 
TATGCCCTGC AGAACCCAGG AATGACCAGG TTCCTGGGGC TCTACGGGGA CGACGATGAC 
TCCTGGTGTT CATCCTCCAC TTCCTCCTCC GACTCAGAAG AAGAAGGCTA TTTTCTTGGA 
CAGCCAATCC CTCAGCCTCG GCCACAAAGA TTCACCTACT ATACGGATGA CCTTTCTAGT 
CCGGCTTCTG CACTGCCCAC CCCACAGTTT AATCAGAGGA CAACTAAATC CAAGAAGAAA 
AAGGGACACA GGGGCAAAAA CTGTATCATT TCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Prickle1 ( XM_006242213.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006242275.1 Rattus norvegicus prickle planar cell polarity protein 1 (Prickle1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa05310
Clone ID Related Accession (Same CDS sequence)	XM_006242213.3 , XM_006242214.3 , XM_017594887.1 , XM_006242215.3 , NM_199396.1
Accession Version	XM_006242213.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2496bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	prickle-like protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005106.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006242213.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTTTGG AGATGGAGCC CAAAATGAGC AAACTGGCCT TTGGGTGCCA GAGAAGTTCC 
ACATCAGACG ACGATTCCGG GTGCGCCCTG GAAGAGTATG CCTGGGTCCC ACCGGGCCTC 
AGGCCTGAGC AGATCCAGCT CTATTTCGCA TGCTTGCCAG AGGAAAAGGT TCCTTACGTT 
AACAGCCCTG GAGAGAAACA CCGGATTAAA CAGCTTTTGT ACCAGTTGCC ACCACACGAT 
AACGAGGTGA GGTACTGCCA GTCTTTAAGC GAAGAGGAGA AGAAGGAATT GCAGGTGTTC 
AGTGCTCAGA GGAAGAAAGA AGCTCTCGGA AGAGGAACCA TTAAACTCTT GTCCAGAGCA 
ATGATGCACG CAGTGTGTGA GCAGTGTGGG TTGCAGATGA ACGGAGGGGA GGTCGCAGTG 
TTTGCCTCCC GTGCGGGTCC TGGAGTGTGC TGGCATCCAT CCTGCTTTGT CTGCTTCACG 
TGTAATGAAC TACTGGTCGA CCTCATCTAT TTCTATCAGG ATGGAAAAAT CCACTGTGGC 
AGGCACCATG CCGAACTGCT CAAACCTCGG TGCTCAGCCT GTGACGAGAT CATTTTTGCG 
GACGAGTGCA CAGAGGCCGA GGGCCGCCAC TGGCACATGA AGCATTTCTG CTGCCTAGAA 
TGTGAAACGG TCCTGGGAGG GCAGAGATAT ATCATGAAGG ATGGCCGCCC CTTCTGCTGC 
GGCTGCTTCG AGTCTCTCTA CGCGGAGTAC TGCGAAACCT GTGGCGAGCA TATTGGTGTG 
GACCATGCCC AGATGACCTA TGACGGACAA CATTGGCATG CCACCGAGGC CTGCTTTTCC 
TGTGCTCAAT GTAAGGCCTC TTTGCTGGGA TGCCCCTTCC TCCCAAAACA AGGTCAGATC 
TACTGCTCCA AGACCTGCAG CCTTGGGGAA GACATCCACG CCTCCGATTC CTCTGACTCT 
GCCTTCCAGT CAGCCCGATC GAGAGACTCC CGGAGAAGTG TGAGGATGGG CAGAAGCAGC 
CGCTCTGCTG ACCAGTGCAG GCAGTCTCTC CTTCTGTCTC CCGCTCTGAA CTACAAGTTC 
CCGGGTCTTT CTGGCAGTGC TGACGACACT CTCTCTCGGA AGCTGGACGA CGTGAGCCTC 
TCCGGGCAGG GAGCGGGCTT TGCTCATGAG GAATTCTGGA AAGCCAGAGT GGATCAGGAA 
GCCTCTGAAG ACCCTGAAGA ATGGGCTGAG CACGAAGATT ATATGACACA GCTCCTCCTC 
AAGTTTGGTG ATAAAAACCT CTTCCAGCAG CCGCCCAGCG AGGTGGACAT GAGAGCCAGT 
GAGCACTGGA TACCCGACAA CATGGTTACG AATAAGCCGG AGGCAAAGCA GAATCACCAG 
AGCCTTGCGA GTAAAAAGTA TCAGTCGGAT ATGTACTGGG CCCAGTCCCA AGACGGGCTG 
GGTGACTCCG CCTACGGCAG CCACCCAGGC CCTGCCAGCA GCCGAAGGCT GCAAGAGCTA 
GATCTGGACC ACGGTGCTGC GGGGTATAAC CATGACCAAA CCCAGTGGTA TGAAGACTCC 
CTGGAGTGCC TGTCTGACTT GAAACCAGAG CAGAGTGTCC GGGATTCTAT GGATTCTCTG 
GCGTTGTCCA ACATCACAGG GGCATCCGTG GACGGAGAAA GCAAGCCAAG GCCATCATTA 
TACTCTCTCC AAAACTTCGA GGAGATAGAG GCAGAAGACT GTGAGAAAAT GAGCAATATG 
GGAACTCTGA ATTCTTCCAT GCTGCACAGG AGCGCAGAGT CCTTAAAAAG TCTGAATTCA 
GAACTGTGCC CAGAAAAAAT TATACCGGAG GAAAAACCAG TGCACCTGCC GGTGCTCAGA 
AGATCCAAGT CTCAGTCCCG ACCACAGCAG GTCAAGTTTT CAGATGACGT CATCGACAAC 
GGAAGCTACG ACATCGAAAT CCGGCAGCCT CCAATGAGTG AAAGGACTCG GAGACGAGTG 
TATCACTTTG AAGAGAGGGG ATCCAGGCCT CACCACCATC GCCACCGGAG AAGCAGAAAG 
TCGCGGTCCG ACAACGCCCT GAACCTGGTC ACAGAAAGGA AGTACTCCGC CAAGGACAGA 
CTTCGGCTGT ACACCCCCGA TAACTATGAG AAATTCATAC AGAGTAAGGG CGCCCGGGAG 
CTCCAAGCAT ACATGCAGAA TGCCAACCTC TACGGCCAGT ATGCCCACAC CACATCGGAT 
TATGCCCTGC AGAACCCAGG AATGACCAGG TTCCTGGGGC TCTACGGGGA CGACGATGAC 
TCCTGGTGTT CATCCTCCAC TTCCTCCTCC GACTCAGAAG AAGAAGGCTA TTTTCTTGGA 
CAGCCAATCC CTCAGCCTCG GCCACAAAGA TTCACCTACT ATACGGATGA CCTTTCTAGT 
CCGGCTTCTG CACTGCCCAC CCCACAGTTT AATCAGAGGA CAACTAAATC CAAGAAGAAA 
AAGGGACACA GGGGCAAAAA CTGTATCATT TCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006242275.1
CDS	255..2750
Translation	MPLEMEPKMSKLAFGCQRSSTSDDDSGCALEEYAWVPPGLRPEQIQLYFACLPEEKVPYVNSPGEKHRIKQLLYQLPPHDNEVRYCQSLSEEEKKELQVFSAQRKKEALGRGTIKLLSRAMMHAVCEQCGLQMNGGEVAVFASRAGPGVCWHPSCFVCFTCNELLVDLIYFYQDGKIHCGRHHAELLKPRCSACDEIIFADECTEAEGRHWHMKHFCCLECETVLGGQRYIMKDGRPFCCGCFESLYAEYCETCGEHIGVDHAQMTYDGQHWHATEACFSCAQCKASLLGCPFLPKQGQIYCSKTCSLGEDIHASDSSDSAFQSARSRDSRRSVRMGRSSRSADQCRQSLLLSPALNYKFPGLSGSADDTLSRKLDDVSLSGQGAGFAHEEFWKARVDQEASEDPEEWAEHEDYMTQLLLKFGDKNLFQQPPSEVDMRASEHWIPDNMVTNKPEAKQNHQSLASKKYQSDMYWAQSQDGLGDSAYGSHPGPASSRRLQELDLDHGAAGYNHDQTQWYEDSLECLSDLKPEQSVRDSMDSLALSNITGASVDGESKPRPSLYSLQNFEEIEAEDCEKMSNMGTLNSSMLHRSAESLKSLNSELCPEKIIPEEKPVHLPVLRRSKSQSRPQQVKFSDDVIDNGSYDIEIRQPPMSERTRRRVYHFEERGSRPHHHRHRRSRKSRSDNALNLVTERKYSAKDRLRLYTPDNYEKFIQSKGARELQAYMQNANLYGQYAHTTSDYALQNPGMTRFLGLYGDDDDSWCSSSTSSSDSEEEGYFLGQPIPQPRPQRFTYYTDDLSSPASALPTPQFNQRTTKSKKKKGHRGKNCIIS

Target ORF information:

RefSeq Version	XM_006242213.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus prickle planar cell polarity protein 1 (Prickle1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006242213.3

ORF Insert Sequence:

ATGCCTTTGG AGATGGAGCC CAAAATGAGC AAACTGGCCT TTGGGTGCCA GAGAAGTTCC 
ACATCAGACG ACGATTCCGG GTGCGCCCTG GAAGAGTATG CCTGGGTCCC ACCGGGCCTC 
AGGCCTGAGC AGATCCAGCT CTATTTCGCA TGCTTGCCAG AGGAAAAGGT TCCTTACGTT 
AACAGCCCTG GAGAGAAACA CCGGATTAAA CAGCTTTTGT ACCAGTTGCC ACCACACGAT 
AACGAGGTGA GGTACTGCCA GTCTTTAAGC GAAGAGGAGA AGAAGGAATT GCAGGTGTTC 
AGTGCTCAGA GGAAGAAAGA AGCTCTCGGA AGAGGAACCA TTAAACTCTT GTCCAGAGCA 
ATGATGCACG CAGTGTGTGA GCAGTGTGGG TTGCAGATGA ACGGAGGGGA GGTCGCAGTG 
TTTGCCTCCC GTGCGGGTCC TGGAGTGTGC TGGCATCCAT CCTGCTTTGT CTGCTTCACG 
TGTAATGAAC TACTGGTCGA CCTCATCTAT TTCTATCAGG ATGGAAAAAT CCACTGTGGC 
AGGCACCATG CCGAACTGCT CAAACCTCGG TGCTCAGCCT GTGACGAGAT CATTTTTGCG 
GACGAGTGCA CAGAGGCCGA GGGCCGCCAC TGGCACATGA AGCATTTCTG CTGCCTAGAA 
TGTGAAACGG TCCTGGGAGG GCAGAGATAT ATCATGAAGG ATGGCCGCCC CTTCTGCTGC 
GGCTGCTTCG AGTCTCTCTA CGCGGAGTAC TGCGAAACCT GTGGCGAGCA TATTGGTGTG 
GACCATGCCC AGATGACCTA TGACGGACAA CATTGGCATG CCACCGAGGC CTGCTTTTCC 
TGTGCTCAAT GTAAGGCCTC TTTGCTGGGA TGCCCCTTCC TCCCAAAACA AGGTCAGATC 
TACTGCTCCA AGACCTGCAG CCTTGGGGAA GACATCCACG CCTCCGATTC CTCTGACTCT 
GCCTTCCAGT CAGCCCGATC GAGAGACTCC CGGAGAAGTG TGAGGATGGG CAGAAGCAGC 
CGCTCTGCTG ACCAGTGCAG GCAGTCTCTC CTTCTGTCTC CCGCTCTGAA CTACAAGTTC 
CCGGGTCTTT CTGGCAGTGC TGACGACACT CTCTCTCGGA AGCTGGACGA CGTGAGCCTC 
TCCGGGCAGG GAGCGGGCTT TGCTCATGAG GAATTCTGGA AAGCCAGAGT GGATCAGGAA 
GCCTCTGAAG ACCCTGAAGA ATGGGCTGAG CACGAAGATT ATATGACACA GCTCCTCCTC 
AAGTTTGGTG ATAAAAACCT CTTCCAGCAG CCGCCCAGCG AGGTGGACAT GAGAGCCAGT 
GAGCACTGGA TACCCGACAA CATGGTTACG AATAAGCCGG AGGCAAAGCA GAATCACCAG 
AGCCTTGCGA GTAAAAAGTA TCAGTCGGAT ATGTACTGGG CCCAGTCCCA AGACGGGCTG 
GGTGACTCCG CCTACGGCAG CCACCCAGGC CCTGCCAGCA GCCGAAGGCT GCAAGAGCTA 
GATCTGGACC ACGGTGCTGC GGGGTATAAC CATGACCAAA CCCAGTGGTA TGAAGACTCC 
CTGGAGTGCC TGTCTGACTT GAAACCAGAG CAGAGTGTCC GGGATTCTAT GGATTCTCTG 
GCGTTGTCCA ACATCACAGG GGCATCCGTG GACGGAGAAA GCAAGCCAAG GCCATCATTA 
TACTCTCTCC AAAACTTCGA GGAGATAGAG GCAGAAGACT GTGAGAAAAT GAGCAATATG 
GGAACTCTGA ATTCTTCCAT GCTGCACAGG AGCGCAGAGT CCTTAAAAAG TCTGAATTCA 
GAACTGTGCC CAGAAAAAAT TATACCGGAG GAAAAACCAG TGCACCTGCC GGTGCTCAGA 
AGATCCAAGT CTCAGTCCCG ACCACAGCAG GTCAAGTTTT CAGATGACGT CATCGACAAC 
GGAAGCTACG ACATCGAAAT CCGGCAGCCT CCAATGAGTG AAAGGACTCG GAGACGAGTG 
TATCACTTTG AAGAGAGGGG ATCCAGGCCT CACCACCATC GCCACCGGAG AAGCAGAAAG 
TCGCGGTCCG ACAACGCCCT GAACCTGGTC ACAGAAAGGA AGTACTCCGC CAAGGACAGA 
CTTCGGCTGT ACACCCCCGA TAACTATGAG AAATTCATAC AGAGTAAGGG CGCCCGGGAG 
CTCCAAGCAT ACATGCAGAA TGCCAACCTC TACGGCCAGT ATGCCCACAC CACATCGGAT 
TATGCCCTGC AGAACCCAGG AATGACCAGG TTCCTGGGGC TCTACGGGGA CGACGATGAC 
TCCTGGTGTT CATCCTCCAC TTCCTCCTCC GACTCAGAAG AAGAAGGCTA TTTTCTTGGA 
CAGCCAATCC CTCAGCCTCG GCCACAAAGA TTCACCTACT ATACGGATGA CCTTTCTAGT 
CCGGCTTCTG CACTGCCCAC CCCACAGTTT AATCAGAGGA CAACTAAATC CAAGAAGAAA 
AAGGGACACA GGGGCAAAAA CTGTATCATT TCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Prickle1 ( XM_006242214.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006242276.1 Rattus norvegicus prickle planar cell polarity protein 1 (Prickle1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa05310
Clone ID Related Accession (Same CDS sequence)	XM_006242213.3 , XM_006242214.3 , XM_017594887.1 , XM_006242215.3 , NM_199396.1
Accession Version	XM_006242214.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2496bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	prickle-like protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005106.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006242214.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTTTGG AGATGGAGCC CAAAATGAGC AAACTGGCCT TTGGGTGCCA GAGAAGTTCC 
ACATCAGACG ACGATTCCGG GTGCGCCCTG GAAGAGTATG CCTGGGTCCC ACCGGGCCTC 
AGGCCTGAGC AGATCCAGCT CTATTTCGCA TGCTTGCCAG AGGAAAAGGT TCCTTACGTT 
AACAGCCCTG GAGAGAAACA CCGGATTAAA CAGCTTTTGT ACCAGTTGCC ACCACACGAT 
AACGAGGTGA GGTACTGCCA GTCTTTAAGC GAAGAGGAGA AGAAGGAATT GCAGGTGTTC 
AGTGCTCAGA GGAAGAAAGA AGCTCTCGGA AGAGGAACCA TTAAACTCTT GTCCAGAGCA 
ATGATGCACG CAGTGTGTGA GCAGTGTGGG TTGCAGATGA ACGGAGGGGA GGTCGCAGTG 
TTTGCCTCCC GTGCGGGTCC TGGAGTGTGC TGGCATCCAT CCTGCTTTGT CTGCTTCACG 
TGTAATGAAC TACTGGTCGA CCTCATCTAT TTCTATCAGG ATGGAAAAAT CCACTGTGGC 
AGGCACCATG CCGAACTGCT CAAACCTCGG TGCTCAGCCT GTGACGAGAT CATTTTTGCG 
GACGAGTGCA CAGAGGCCGA GGGCCGCCAC TGGCACATGA AGCATTTCTG CTGCCTAGAA 
TGTGAAACGG TCCTGGGAGG GCAGAGATAT ATCATGAAGG ATGGCCGCCC CTTCTGCTGC 
GGCTGCTTCG AGTCTCTCTA CGCGGAGTAC TGCGAAACCT GTGGCGAGCA TATTGGTGTG 
GACCATGCCC AGATGACCTA TGACGGACAA CATTGGCATG CCACCGAGGC CTGCTTTTCC 
TGTGCTCAAT GTAAGGCCTC TTTGCTGGGA TGCCCCTTCC TCCCAAAACA AGGTCAGATC 
TACTGCTCCA AGACCTGCAG CCTTGGGGAA GACATCCACG CCTCCGATTC CTCTGACTCT 
GCCTTCCAGT CAGCCCGATC GAGAGACTCC CGGAGAAGTG TGAGGATGGG CAGAAGCAGC 
CGCTCTGCTG ACCAGTGCAG GCAGTCTCTC CTTCTGTCTC CCGCTCTGAA CTACAAGTTC 
CCGGGTCTTT CTGGCAGTGC TGACGACACT CTCTCTCGGA AGCTGGACGA CGTGAGCCTC 
TCCGGGCAGG GAGCGGGCTT TGCTCATGAG GAATTCTGGA AAGCCAGAGT GGATCAGGAA 
GCCTCTGAAG ACCCTGAAGA ATGGGCTGAG CACGAAGATT ATATGACACA GCTCCTCCTC 
AAGTTTGGTG ATAAAAACCT CTTCCAGCAG CCGCCCAGCG AGGTGGACAT GAGAGCCAGT 
GAGCACTGGA TACCCGACAA CATGGTTACG AATAAGCCGG AGGCAAAGCA GAATCACCAG 
AGCCTTGCGA GTAAAAAGTA TCAGTCGGAT ATGTACTGGG CCCAGTCCCA AGACGGGCTG 
GGTGACTCCG CCTACGGCAG CCACCCAGGC CCTGCCAGCA GCCGAAGGCT GCAAGAGCTA 
GATCTGGACC ACGGTGCTGC GGGGTATAAC CATGACCAAA CCCAGTGGTA TGAAGACTCC 
CTGGAGTGCC TGTCTGACTT GAAACCAGAG CAGAGTGTCC GGGATTCTAT GGATTCTCTG 
GCGTTGTCCA ACATCACAGG GGCATCCGTG GACGGAGAAA GCAAGCCAAG GCCATCATTA 
TACTCTCTCC AAAACTTCGA GGAGATAGAG GCAGAAGACT GTGAGAAAAT GAGCAATATG 
GGAACTCTGA ATTCTTCCAT GCTGCACAGG AGCGCAGAGT CCTTAAAAAG TCTGAATTCA 
GAACTGTGCC CAGAAAAAAT TATACCGGAG GAAAAACCAG TGCACCTGCC GGTGCTCAGA 
AGATCCAAGT CTCAGTCCCG ACCACAGCAG GTCAAGTTTT CAGATGACGT CATCGACAAC 
GGAAGCTACG ACATCGAAAT CCGGCAGCCT CCAATGAGTG AAAGGACTCG GAGACGAGTG 
TATCACTTTG AAGAGAGGGG ATCCAGGCCT CACCACCATC GCCACCGGAG AAGCAGAAAG 
TCGCGGTCCG ACAACGCCCT GAACCTGGTC ACAGAAAGGA AGTACTCCGC CAAGGACAGA 
CTTCGGCTGT ACACCCCCGA TAACTATGAG AAATTCATAC AGAGTAAGGG CGCCCGGGAG 
CTCCAAGCAT ACATGCAGAA TGCCAACCTC TACGGCCAGT ATGCCCACAC CACATCGGAT 
TATGCCCTGC AGAACCCAGG AATGACCAGG TTCCTGGGGC TCTACGGGGA CGACGATGAC 
TCCTGGTGTT CATCCTCCAC TTCCTCCTCC GACTCAGAAG AAGAAGGCTA TTTTCTTGGA 
CAGCCAATCC CTCAGCCTCG GCCACAAAGA TTCACCTACT ATACGGATGA CCTTTCTAGT 
CCGGCTTCTG CACTGCCCAC CCCACAGTTT AATCAGAGGA CAACTAAATC CAAGAAGAAA 
AAGGGACACA GGGGCAAAAA CTGTATCATT TCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006242276.1
CDS	2530..5025
Translation	MPLEMEPKMSKLAFGCQRSSTSDDDSGCALEEYAWVPPGLRPEQIQLYFACLPEEKVPYVNSPGEKHRIKQLLYQLPPHDNEVRYCQSLSEEEKKELQVFSAQRKKEALGRGTIKLLSRAMMHAVCEQCGLQMNGGEVAVFASRAGPGVCWHPSCFVCFTCNELLVDLIYFYQDGKIHCGRHHAELLKPRCSACDEIIFADECTEAEGRHWHMKHFCCLECETVLGGQRYIMKDGRPFCCGCFESLYAEYCETCGEHIGVDHAQMTYDGQHWHATEACFSCAQCKASLLGCPFLPKQGQIYCSKTCSLGEDIHASDSSDSAFQSARSRDSRRSVRMGRSSRSADQCRQSLLLSPALNYKFPGLSGSADDTLSRKLDDVSLSGQGAGFAHEEFWKARVDQEASEDPEEWAEHEDYMTQLLLKFGDKNLFQQPPSEVDMRASEHWIPDNMVTNKPEAKQNHQSLASKKYQSDMYWAQSQDGLGDSAYGSHPGPASSRRLQELDLDHGAAGYNHDQTQWYEDSLECLSDLKPEQSVRDSMDSLALSNITGASVDGESKPRPSLYSLQNFEEIEAEDCEKMSNMGTLNSSMLHRSAESLKSLNSELCPEKIIPEEKPVHLPVLRRSKSQSRPQQVKFSDDVIDNGSYDIEIRQPPMSERTRRRVYHFEERGSRPHHHRHRRSRKSRSDNALNLVTERKYSAKDRLRLYTPDNYEKFIQSKGARELQAYMQNANLYGQYAHTTSDYALQNPGMTRFLGLYGDDDDSWCSSSTSSSDSEEEGYFLGQPIPQPRPQRFTYYTDDLSSPASALPTPQFNQRTTKSKKKKGHRGKNCIIS

Target ORF information:

RefSeq Version	XM_006242214.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus prickle planar cell polarity protein 1 (Prickle1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006242214.3

ORF Insert Sequence:

ATGCCTTTGG AGATGGAGCC CAAAATGAGC AAACTGGCCT TTGGGTGCCA GAGAAGTTCC 
ACATCAGACG ACGATTCCGG GTGCGCCCTG GAAGAGTATG CCTGGGTCCC ACCGGGCCTC 
AGGCCTGAGC AGATCCAGCT CTATTTCGCA TGCTTGCCAG AGGAAAAGGT TCCTTACGTT 
AACAGCCCTG GAGAGAAACA CCGGATTAAA CAGCTTTTGT ACCAGTTGCC ACCACACGAT 
AACGAGGTGA GGTACTGCCA GTCTTTAAGC GAAGAGGAGA AGAAGGAATT GCAGGTGTTC 
AGTGCTCAGA GGAAGAAAGA AGCTCTCGGA AGAGGAACCA TTAAACTCTT GTCCAGAGCA 
ATGATGCACG CAGTGTGTGA GCAGTGTGGG TTGCAGATGA ACGGAGGGGA GGTCGCAGTG 
TTTGCCTCCC GTGCGGGTCC TGGAGTGTGC TGGCATCCAT CCTGCTTTGT CTGCTTCACG 
TGTAATGAAC TACTGGTCGA CCTCATCTAT TTCTATCAGG ATGGAAAAAT CCACTGTGGC 
AGGCACCATG CCGAACTGCT CAAACCTCGG TGCTCAGCCT GTGACGAGAT CATTTTTGCG 
GACGAGTGCA CAGAGGCCGA GGGCCGCCAC TGGCACATGA AGCATTTCTG CTGCCTAGAA 
TGTGAAACGG TCCTGGGAGG GCAGAGATAT ATCATGAAGG ATGGCCGCCC CTTCTGCTGC 
GGCTGCTTCG AGTCTCTCTA CGCGGAGTAC TGCGAAACCT GTGGCGAGCA TATTGGTGTG 
GACCATGCCC AGATGACCTA TGACGGACAA CATTGGCATG CCACCGAGGC CTGCTTTTCC 
TGTGCTCAAT GTAAGGCCTC TTTGCTGGGA TGCCCCTTCC TCCCAAAACA AGGTCAGATC 
TACTGCTCCA AGACCTGCAG CCTTGGGGAA GACATCCACG CCTCCGATTC CTCTGACTCT 
GCCTTCCAGT CAGCCCGATC GAGAGACTCC CGGAGAAGTG TGAGGATGGG CAGAAGCAGC 
CGCTCTGCTG ACCAGTGCAG GCAGTCTCTC CTTCTGTCTC CCGCTCTGAA CTACAAGTTC 
CCGGGTCTTT CTGGCAGTGC TGACGACACT CTCTCTCGGA AGCTGGACGA CGTGAGCCTC 
TCCGGGCAGG GAGCGGGCTT TGCTCATGAG GAATTCTGGA AAGCCAGAGT GGATCAGGAA 
GCCTCTGAAG ACCCTGAAGA ATGGGCTGAG CACGAAGATT ATATGACACA GCTCCTCCTC 
AAGTTTGGTG ATAAAAACCT CTTCCAGCAG CCGCCCAGCG AGGTGGACAT GAGAGCCAGT 
GAGCACTGGA TACCCGACAA CATGGTTACG AATAAGCCGG AGGCAAAGCA GAATCACCAG 
AGCCTTGCGA GTAAAAAGTA TCAGTCGGAT ATGTACTGGG CCCAGTCCCA AGACGGGCTG 
GGTGACTCCG CCTACGGCAG CCACCCAGGC CCTGCCAGCA GCCGAAGGCT GCAAGAGCTA 
GATCTGGACC ACGGTGCTGC GGGGTATAAC CATGACCAAA CCCAGTGGTA TGAAGACTCC 
CTGGAGTGCC TGTCTGACTT GAAACCAGAG CAGAGTGTCC GGGATTCTAT GGATTCTCTG 
GCGTTGTCCA ACATCACAGG GGCATCCGTG GACGGAGAAA GCAAGCCAAG GCCATCATTA 
TACTCTCTCC AAAACTTCGA GGAGATAGAG GCAGAAGACT GTGAGAAAAT GAGCAATATG 
GGAACTCTGA ATTCTTCCAT GCTGCACAGG AGCGCAGAGT CCTTAAAAAG TCTGAATTCA 
GAACTGTGCC CAGAAAAAAT TATACCGGAG GAAAAACCAG TGCACCTGCC GGTGCTCAGA 
AGATCCAAGT CTCAGTCCCG ACCACAGCAG GTCAAGTTTT CAGATGACGT CATCGACAAC 
GGAAGCTACG ACATCGAAAT CCGGCAGCCT CCAATGAGTG AAAGGACTCG GAGACGAGTG 
TATCACTTTG AAGAGAGGGG ATCCAGGCCT CACCACCATC GCCACCGGAG AAGCAGAAAG 
TCGCGGTCCG ACAACGCCCT GAACCTGGTC ACAGAAAGGA AGTACTCCGC CAAGGACAGA 
CTTCGGCTGT ACACCCCCGA TAACTATGAG AAATTCATAC AGAGTAAGGG CGCCCGGGAG 
CTCCAAGCAT ACATGCAGAA TGCCAACCTC TACGGCCAGT ATGCCCACAC CACATCGGAT 
TATGCCCTGC AGAACCCAGG AATGACCAGG TTCCTGGGGC TCTACGGGGA CGACGATGAC 
TCCTGGTGTT CATCCTCCAC TTCCTCCTCC GACTCAGAAG AAGAAGGCTA TTTTCTTGGA 
CAGCCAATCC CTCAGCCTCG GCCACAAAGA TTCACCTACT ATACGGATGA CCTTTCTAGT 
CCGGCTTCTG CACTGCCCAC CCCACAGTTT AATCAGAGGA CAACTAAATC CAAGAAGAAA 
AAGGGACACA GGGGCAAAAA CTGTATCATT TCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Prickle1 ( XM_017594887.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017450376.1 Rattus norvegicus prickle planar cell polarity protein 1 (Prickle1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ORa05310
Clone ID Related Accession (Same CDS sequence)	XM_006242213.3 , XM_006242214.3 , XM_017594887.1 , XM_006242215.3 , NM_199396.1
Accession Version	XM_017594887.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2496bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	prickle-like protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005106.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTTTGG AGATGGAGCC CAAAATGAGC AAACTGGCCT TTGGGTGCCA GAGAAGTTCC 
ACATCAGACG ACGATTCCGG GTGCGCCCTG GAAGAGTATG CCTGGGTCCC ACCGGGCCTC 
AGGCCTGAGC AGATCCAGCT CTATTTCGCA TGCTTGCCAG AGGAAAAGGT TCCTTACGTT 
AACAGCCCTG GAGAGAAACA CCGGATTAAA CAGCTTTTGT ACCAGTTGCC ACCACACGAT 
AACGAGGTGA GGTACTGCCA GTCTTTAAGC GAAGAGGAGA AGAAGGAATT GCAGGTGTTC 
AGTGCTCAGA GGAAGAAAGA AGCTCTCGGA AGAGGAACCA TTAAACTCTT GTCCAGAGCA 
ATGATGCACG CAGTGTGTGA GCAGTGTGGG TTGCAGATGA ACGGAGGGGA GGTCGCAGTG 
TTTGCCTCCC GTGCGGGTCC TGGAGTGTGC TGGCATCCAT CCTGCTTTGT CTGCTTCACG 
TGTAATGAAC TACTGGTCGA CCTCATCTAT TTCTATCAGG ATGGAAAAAT CCACTGTGGC 
AGGCACCATG CCGAACTGCT CAAACCTCGG TGCTCAGCCT GTGACGAGAT CATTTTTGCG 
GACGAGTGCA CAGAGGCCGA GGGCCGCCAC TGGCACATGA AGCATTTCTG CTGCCTAGAA 
TGTGAAACGG TCCTGGGAGG GCAGAGATAT ATCATGAAGG ATGGCCGCCC CTTCTGCTGC 
GGCTGCTTCG AGTCTCTCTA CGCGGAGTAC TGCGAAACCT GTGGCGAGCA TATTGGTGTG 
GACCATGCCC AGATGACCTA TGACGGACAA CATTGGCATG CCACCGAGGC CTGCTTTTCC 
TGTGCTCAAT GTAAGGCCTC TTTGCTGGGA TGCCCCTTCC TCCCAAAACA AGGTCAGATC 
TACTGCTCCA AGACCTGCAG CCTTGGGGAA GACATCCACG CCTCCGATTC CTCTGACTCT 
GCCTTCCAGT CAGCCCGATC GAGAGACTCC CGGAGAAGTG TGAGGATGGG CAGAAGCAGC 
CGCTCTGCTG ACCAGTGCAG GCAGTCTCTC CTTCTGTCTC CCGCTCTGAA CTACAAGTTC 
CCGGGTCTTT CTGGCAGTGC TGACGACACT CTCTCTCGGA AGCTGGACGA CGTGAGCCTC 
TCCGGGCAGG GAGCGGGCTT TGCTCATGAG GAATTCTGGA AAGCCAGAGT GGATCAGGAA 
GCCTCTGAAG ACCCTGAAGA ATGGGCTGAG CACGAAGATT ATATGACACA GCTCCTCCTC 
AAGTTTGGTG ATAAAAACCT CTTCCAGCAG CCGCCCAGCG AGGTGGACAT GAGAGCCAGT 
GAGCACTGGA TACCCGACAA CATGGTTACG AATAAGCCGG AGGCAAAGCA GAATCACCAG 
AGCCTTGCGA GTAAAAAGTA TCAGTCGGAT ATGTACTGGG CCCAGTCCCA AGACGGGCTG 
GGTGACTCCG CCTACGGCAG CCACCCAGGC CCTGCCAGCA GCCGAAGGCT GCAAGAGCTA 
GATCTGGACC ACGGTGCTGC GGGGTATAAC CATGACCAAA CCCAGTGGTA TGAAGACTCC 
CTGGAGTGCC TGTCTGACTT GAAACCAGAG CAGAGTGTCC GGGATTCTAT GGATTCTCTG 
GCGTTGTCCA ACATCACAGG GGCATCCGTG GACGGAGAAA GCAAGCCAAG GCCATCATTA 
TACTCTCTCC AAAACTTCGA GGAGATAGAG GCAGAAGACT GTGAGAAAAT GAGCAATATG 
GGAACTCTGA ATTCTTCCAT GCTGCACAGG AGCGCAGAGT CCTTAAAAAG TCTGAATTCA 
GAACTGTGCC CAGAAAAAAT TATACCGGAG GAAAAACCAG TGCACCTGCC GGTGCTCAGA 
AGATCCAAGT CTCAGTCCCG ACCACAGCAG GTCAAGTTTT CAGATGACGT CATCGACAAC 
GGAAGCTACG ACATCGAAAT CCGGCAGCCT CCAATGAGTG AAAGGACTCG GAGACGAGTG 
TATCACTTTG AAGAGAGGGG ATCCAGGCCT CACCACCATC GCCACCGGAG AAGCAGAAAG 
TCGCGGTCCG ACAACGCCCT GAACCTGGTC ACAGAAAGGA AGTACTCCGC CAAGGACAGA 
CTTCGGCTGT ACACCCCCGA TAACTATGAG AAATTCATAC AGAGTAAGGG CGCCCGGGAG 
CTCCAAGCAT ACATGCAGAA TGCCAACCTC TACGGCCAGT ATGCCCACAC CACATCGGAT 
TATGCCCTGC AGAACCCAGG AATGACCAGG TTCCTGGGGC TCTACGGGGA CGACGATGAC 
TCCTGGTGTT CATCCTCCAC TTCCTCCTCC GACTCAGAAG AAGAAGGCTA TTTTCTTGGA 
CAGCCAATCC CTCAGCCTCG GCCACAAAGA TTCACCTACT ATACGGATGA CCTTTCTAGT 
CCGGCTTCTG CACTGCCCAC CCCACAGTTT AATCAGAGGA CAACTAAATC CAAGAAGAAA 
AAGGGACACA GGGGCAAAAA CTGTATCATT TCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017450376.1
CDS	339..2834
Translation	MPLEMEPKMSKLAFGCQRSSTSDDDSGCALEEYAWVPPGLRPEQIQLYFACLPEEKVPYVNSPGEKHRIKQLLYQLPPHDNEVRYCQSLSEEEKKELQVFSAQRKKEALGRGTIKLLSRAMMHAVCEQCGLQMNGGEVAVFASRAGPGVCWHPSCFVCFTCNELLVDLIYFYQDGKIHCGRHHAELLKPRCSACDEIIFADECTEAEGRHWHMKHFCCLECETVLGGQRYIMKDGRPFCCGCFESLYAEYCETCGEHIGVDHAQMTYDGQHWHATEACFSCAQCKASLLGCPFLPKQGQIYCSKTCSLGEDIHASDSSDSAFQSARSRDSRRSVRMGRSSRSADQCRQSLLLSPALNYKFPGLSGSADDTLSRKLDDVSLSGQGAGFAHEEFWKARVDQEASEDPEEWAEHEDYMTQLLLKFGDKNLFQQPPSEVDMRASEHWIPDNMVTNKPEAKQNHQSLASKKYQSDMYWAQSQDGLGDSAYGSHPGPASSRRLQELDLDHGAAGYNHDQTQWYEDSLECLSDLKPEQSVRDSMDSLALSNITGASVDGESKPRPSLYSLQNFEEIEAEDCEKMSNMGTLNSSMLHRSAESLKSLNSELCPEKIIPEEKPVHLPVLRRSKSQSRPQQVKFSDDVIDNGSYDIEIRQPPMSERTRRRVYHFEERGSRPHHHRHRRSRKSRSDNALNLVTERKYSAKDRLRLYTPDNYEKFIQSKGARELQAYMQNANLYGQYAHTTSDYALQNPGMTRFLGLYGDDDDSWCSSSTSSSDSEEEGYFLGQPIPQPRPQRFTYYTDDLSSPASALPTPQFNQRTTKSKKKKGHRGKNCIIS

Target ORF information:

RefSeq Version	XM_017594887.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus prickle planar cell polarity protein 1 (Prickle1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017594887.1

ORF Insert Sequence:

ATGCCTTTGG AGATGGAGCC CAAAATGAGC AAACTGGCCT TTGGGTGCCA GAGAAGTTCC 
ACATCAGACG ACGATTCCGG GTGCGCCCTG GAAGAGTATG CCTGGGTCCC ACCGGGCCTC 
AGGCCTGAGC AGATCCAGCT CTATTTCGCA TGCTTGCCAG AGGAAAAGGT TCCTTACGTT 
AACAGCCCTG GAGAGAAACA CCGGATTAAA CAGCTTTTGT ACCAGTTGCC ACCACACGAT 
AACGAGGTGA GGTACTGCCA GTCTTTAAGC GAAGAGGAGA AGAAGGAATT GCAGGTGTTC 
AGTGCTCAGA GGAAGAAAGA AGCTCTCGGA AGAGGAACCA TTAAACTCTT GTCCAGAGCA 
ATGATGCACG CAGTGTGTGA GCAGTGTGGG TTGCAGATGA ACGGAGGGGA GGTCGCAGTG 
TTTGCCTCCC GTGCGGGTCC TGGAGTGTGC TGGCATCCAT CCTGCTTTGT CTGCTTCACG 
TGTAATGAAC TACTGGTCGA CCTCATCTAT TTCTATCAGG ATGGAAAAAT CCACTGTGGC 
AGGCACCATG CCGAACTGCT CAAACCTCGG TGCTCAGCCT GTGACGAGAT CATTTTTGCG 
GACGAGTGCA CAGAGGCCGA GGGCCGCCAC TGGCACATGA AGCATTTCTG CTGCCTAGAA 
TGTGAAACGG TCCTGGGAGG GCAGAGATAT ATCATGAAGG ATGGCCGCCC CTTCTGCTGC 
GGCTGCTTCG AGTCTCTCTA CGCGGAGTAC TGCGAAACCT GTGGCGAGCA TATTGGTGTG 
GACCATGCCC AGATGACCTA TGACGGACAA CATTGGCATG CCACCGAGGC CTGCTTTTCC 
TGTGCTCAAT GTAAGGCCTC TTTGCTGGGA TGCCCCTTCC TCCCAAAACA AGGTCAGATC 
TACTGCTCCA AGACCTGCAG CCTTGGGGAA GACATCCACG CCTCCGATTC CTCTGACTCT 
GCCTTCCAGT CAGCCCGATC GAGAGACTCC CGGAGAAGTG TGAGGATGGG CAGAAGCAGC 
CGCTCTGCTG ACCAGTGCAG GCAGTCTCTC CTTCTGTCTC CCGCTCTGAA CTACAAGTTC 
CCGGGTCTTT CTGGCAGTGC TGACGACACT CTCTCTCGGA AGCTGGACGA CGTGAGCCTC 
TCCGGGCAGG GAGCGGGCTT TGCTCATGAG GAATTCTGGA AAGCCAGAGT GGATCAGGAA 
GCCTCTGAAG ACCCTGAAGA ATGGGCTGAG CACGAAGATT ATATGACACA GCTCCTCCTC 
AAGTTTGGTG ATAAAAACCT CTTCCAGCAG CCGCCCAGCG AGGTGGACAT GAGAGCCAGT 
GAGCACTGGA TACCCGACAA CATGGTTACG AATAAGCCGG AGGCAAAGCA GAATCACCAG 
AGCCTTGCGA GTAAAAAGTA TCAGTCGGAT ATGTACTGGG CCCAGTCCCA AGACGGGCTG 
GGTGACTCCG CCTACGGCAG CCACCCAGGC CCTGCCAGCA GCCGAAGGCT GCAAGAGCTA 
GATCTGGACC ACGGTGCTGC GGGGTATAAC CATGACCAAA CCCAGTGGTA TGAAGACTCC 
CTGGAGTGCC TGTCTGACTT GAAACCAGAG CAGAGTGTCC GGGATTCTAT GGATTCTCTG 
GCGTTGTCCA ACATCACAGG GGCATCCGTG GACGGAGAAA GCAAGCCAAG GCCATCATTA 
TACTCTCTCC AAAACTTCGA GGAGATAGAG GCAGAAGACT GTGAGAAAAT GAGCAATATG 
GGAACTCTGA ATTCTTCCAT GCTGCACAGG AGCGCAGAGT CCTTAAAAAG TCTGAATTCA 
GAACTGTGCC CAGAAAAAAT TATACCGGAG GAAAAACCAG TGCACCTGCC GGTGCTCAGA 
AGATCCAAGT CTCAGTCCCG ACCACAGCAG GTCAAGTTTT CAGATGACGT CATCGACAAC 
GGAAGCTACG ACATCGAAAT CCGGCAGCCT CCAATGAGTG AAAGGACTCG GAGACGAGTG 
TATCACTTTG AAGAGAGGGG ATCCAGGCCT CACCACCATC GCCACCGGAG AAGCAGAAAG 
TCGCGGTCCG ACAACGCCCT GAACCTGGTC ACAGAAAGGA AGTACTCCGC CAAGGACAGA 
CTTCGGCTGT ACACCCCCGA TAACTATGAG AAATTCATAC AGAGTAAGGG CGCCCGGGAG 
CTCCAAGCAT ACATGCAGAA TGCCAACCTC TACGGCCAGT ATGCCCACAC CACATCGGAT 
TATGCCCTGC AGAACCCAGG AATGACCAGG TTCCTGGGGC TCTACGGGGA CGACGATGAC 
TCCTGGTGTT CATCCTCCAC TTCCTCCTCC GACTCAGAAG AAGAAGGCTA TTTTCTTGGA 
CAGCCAATCC CTCAGCCTCG GCCACAAAGA TTCACCTACT ATACGGATGA CCTTTCTAGT 
CCGGCTTCTG CACTGCCCAC CCCACAGTTT AATCAGAGGA CAACTAAATC CAAGAAGAAA 
AAGGGACACA GGGGCAAAAA CTGTATCATT TCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Prickle1 ( XM_006242215.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006242277.1 Rattus norvegicus prickle planar cell polarity protein 1 (Prickle1), transcript variant X4, mRNA.

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CloneID	ORa05310
Clone ID Related Accession (Same CDS sequence)	XM_006242213.3 , XM_006242214.3 , XM_017594887.1 , XM_006242215.3 , NM_199396.1
Accession Version	XM_006242215.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2496bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	prickle-like protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005106.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006242215.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTTTGG AGATGGAGCC CAAAATGAGC AAACTGGCCT TTGGGTGCCA GAGAAGTTCC 
ACATCAGACG ACGATTCCGG GTGCGCCCTG GAAGAGTATG CCTGGGTCCC ACCGGGCCTC 
AGGCCTGAGC AGATCCAGCT CTATTTCGCA TGCTTGCCAG AGGAAAAGGT TCCTTACGTT 
AACAGCCCTG GAGAGAAACA CCGGATTAAA CAGCTTTTGT ACCAGTTGCC ACCACACGAT 
AACGAGGTGA GGTACTGCCA GTCTTTAAGC GAAGAGGAGA AGAAGGAATT GCAGGTGTTC 
AGTGCTCAGA GGAAGAAAGA AGCTCTCGGA AGAGGAACCA TTAAACTCTT GTCCAGAGCA 
ATGATGCACG CAGTGTGTGA GCAGTGTGGG TTGCAGATGA ACGGAGGGGA GGTCGCAGTG 
TTTGCCTCCC GTGCGGGTCC TGGAGTGTGC TGGCATCCAT CCTGCTTTGT CTGCTTCACG 
TGTAATGAAC TACTGGTCGA CCTCATCTAT TTCTATCAGG ATGGAAAAAT CCACTGTGGC 
AGGCACCATG CCGAACTGCT CAAACCTCGG TGCTCAGCCT GTGACGAGAT CATTTTTGCG 
GACGAGTGCA CAGAGGCCGA GGGCCGCCAC TGGCACATGA AGCATTTCTG CTGCCTAGAA 
TGTGAAACGG TCCTGGGAGG GCAGAGATAT ATCATGAAGG ATGGCCGCCC CTTCTGCTGC 
GGCTGCTTCG AGTCTCTCTA CGCGGAGTAC TGCGAAACCT GTGGCGAGCA TATTGGTGTG 
GACCATGCCC AGATGACCTA TGACGGACAA CATTGGCATG CCACCGAGGC CTGCTTTTCC 
TGTGCTCAAT GTAAGGCCTC TTTGCTGGGA TGCCCCTTCC TCCCAAAACA AGGTCAGATC 
TACTGCTCCA AGACCTGCAG CCTTGGGGAA GACATCCACG CCTCCGATTC CTCTGACTCT 
GCCTTCCAGT CAGCCCGATC GAGAGACTCC CGGAGAAGTG TGAGGATGGG CAGAAGCAGC 
CGCTCTGCTG ACCAGTGCAG GCAGTCTCTC CTTCTGTCTC CCGCTCTGAA CTACAAGTTC 
CCGGGTCTTT CTGGCAGTGC TGACGACACT CTCTCTCGGA AGCTGGACGA CGTGAGCCTC 
TCCGGGCAGG GAGCGGGCTT TGCTCATGAG GAATTCTGGA AAGCCAGAGT GGATCAGGAA 
GCCTCTGAAG ACCCTGAAGA ATGGGCTGAG CACGAAGATT ATATGACACA GCTCCTCCTC 
AAGTTTGGTG ATAAAAACCT CTTCCAGCAG CCGCCCAGCG AGGTGGACAT GAGAGCCAGT 
GAGCACTGGA TACCCGACAA CATGGTTACG AATAAGCCGG AGGCAAAGCA GAATCACCAG 
AGCCTTGCGA GTAAAAAGTA TCAGTCGGAT ATGTACTGGG CCCAGTCCCA AGACGGGCTG 
GGTGACTCCG CCTACGGCAG CCACCCAGGC CCTGCCAGCA GCCGAAGGCT GCAAGAGCTA 
GATCTGGACC ACGGTGCTGC GGGGTATAAC CATGACCAAA CCCAGTGGTA TGAAGACTCC 
CTGGAGTGCC TGTCTGACTT GAAACCAGAG CAGAGTGTCC GGGATTCTAT GGATTCTCTG 
GCGTTGTCCA ACATCACAGG GGCATCCGTG GACGGAGAAA GCAAGCCAAG GCCATCATTA 
TACTCTCTCC AAAACTTCGA GGAGATAGAG GCAGAAGACT GTGAGAAAAT GAGCAATATG 
GGAACTCTGA ATTCTTCCAT GCTGCACAGG AGCGCAGAGT CCTTAAAAAG TCTGAATTCA 
GAACTGTGCC CAGAAAAAAT TATACCGGAG GAAAAACCAG TGCACCTGCC GGTGCTCAGA 
AGATCCAAGT CTCAGTCCCG ACCACAGCAG GTCAAGTTTT CAGATGACGT CATCGACAAC 
GGAAGCTACG ACATCGAAAT CCGGCAGCCT CCAATGAGTG AAAGGACTCG GAGACGAGTG 
TATCACTTTG AAGAGAGGGG ATCCAGGCCT CACCACCATC GCCACCGGAG AAGCAGAAAG 
TCGCGGTCCG ACAACGCCCT GAACCTGGTC ACAGAAAGGA AGTACTCCGC CAAGGACAGA 
CTTCGGCTGT ACACCCCCGA TAACTATGAG AAATTCATAC AGAGTAAGGG CGCCCGGGAG 
CTCCAAGCAT ACATGCAGAA TGCCAACCTC TACGGCCAGT ATGCCCACAC CACATCGGAT 
TATGCCCTGC AGAACCCAGG AATGACCAGG TTCCTGGGGC TCTACGGGGA CGACGATGAC 
TCCTGGTGTT CATCCTCCAC TTCCTCCTCC GACTCAGAAG AAGAAGGCTA TTTTCTTGGA 
CAGCCAATCC CTCAGCCTCG GCCACAAAGA TTCACCTACT ATACGGATGA CCTTTCTAGT 
CCGGCTTCTG CACTGCCCAC CCCACAGTTT AATCAGAGGA CAACTAAATC CAAGAAGAAA 
AAGGGACACA GGGGCAAAAA CTGTATCATT TCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006242277.1
CDS	456..2951
Translation	MPLEMEPKMSKLAFGCQRSSTSDDDSGCALEEYAWVPPGLRPEQIQLYFACLPEEKVPYVNSPGEKHRIKQLLYQLPPHDNEVRYCQSLSEEEKKELQVFSAQRKKEALGRGTIKLLSRAMMHAVCEQCGLQMNGGEVAVFASRAGPGVCWHPSCFVCFTCNELLVDLIYFYQDGKIHCGRHHAELLKPRCSACDEIIFADECTEAEGRHWHMKHFCCLECETVLGGQRYIMKDGRPFCCGCFESLYAEYCETCGEHIGVDHAQMTYDGQHWHATEACFSCAQCKASLLGCPFLPKQGQIYCSKTCSLGEDIHASDSSDSAFQSARSRDSRRSVRMGRSSRSADQCRQSLLLSPALNYKFPGLSGSADDTLSRKLDDVSLSGQGAGFAHEEFWKARVDQEASEDPEEWAEHEDYMTQLLLKFGDKNLFQQPPSEVDMRASEHWIPDNMVTNKPEAKQNHQSLASKKYQSDMYWAQSQDGLGDSAYGSHPGPASSRRLQELDLDHGAAGYNHDQTQWYEDSLECLSDLKPEQSVRDSMDSLALSNITGASVDGESKPRPSLYSLQNFEEIEAEDCEKMSNMGTLNSSMLHRSAESLKSLNSELCPEKIIPEEKPVHLPVLRRSKSQSRPQQVKFSDDVIDNGSYDIEIRQPPMSERTRRRVYHFEERGSRPHHHRHRRSRKSRSDNALNLVTERKYSAKDRLRLYTPDNYEKFIQSKGARELQAYMQNANLYGQYAHTTSDYALQNPGMTRFLGLYGDDDDSWCSSSTSSSDSEEEGYFLGQPIPQPRPQRFTYYTDDLSSPASALPTPQFNQRTTKSKKKKGHRGKNCIIS

Target ORF information:

RefSeq Version	XM_006242215.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus prickle planar cell polarity protein 1 (Prickle1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006242215.3

ORF Insert Sequence:

ATGCCTTTGG AGATGGAGCC CAAAATGAGC AAACTGGCCT TTGGGTGCCA GAGAAGTTCC 
ACATCAGACG ACGATTCCGG GTGCGCCCTG GAAGAGTATG CCTGGGTCCC ACCGGGCCTC 
AGGCCTGAGC AGATCCAGCT CTATTTCGCA TGCTTGCCAG AGGAAAAGGT TCCTTACGTT 
AACAGCCCTG GAGAGAAACA CCGGATTAAA CAGCTTTTGT ACCAGTTGCC ACCACACGAT 
AACGAGGTGA GGTACTGCCA GTCTTTAAGC GAAGAGGAGA AGAAGGAATT GCAGGTGTTC 
AGTGCTCAGA GGAAGAAAGA AGCTCTCGGA AGAGGAACCA TTAAACTCTT GTCCAGAGCA 
ATGATGCACG CAGTGTGTGA GCAGTGTGGG TTGCAGATGA ACGGAGGGGA GGTCGCAGTG 
TTTGCCTCCC GTGCGGGTCC TGGAGTGTGC TGGCATCCAT CCTGCTTTGT CTGCTTCACG 
TGTAATGAAC TACTGGTCGA CCTCATCTAT TTCTATCAGG ATGGAAAAAT CCACTGTGGC 
AGGCACCATG CCGAACTGCT CAAACCTCGG TGCTCAGCCT GTGACGAGAT CATTTTTGCG 
GACGAGTGCA CAGAGGCCGA GGGCCGCCAC TGGCACATGA AGCATTTCTG CTGCCTAGAA 
TGTGAAACGG TCCTGGGAGG GCAGAGATAT ATCATGAAGG ATGGCCGCCC CTTCTGCTGC 
GGCTGCTTCG AGTCTCTCTA CGCGGAGTAC TGCGAAACCT GTGGCGAGCA TATTGGTGTG 
GACCATGCCC AGATGACCTA TGACGGACAA CATTGGCATG CCACCGAGGC CTGCTTTTCC 
TGTGCTCAAT GTAAGGCCTC TTTGCTGGGA TGCCCCTTCC TCCCAAAACA AGGTCAGATC 
TACTGCTCCA AGACCTGCAG CCTTGGGGAA GACATCCACG CCTCCGATTC CTCTGACTCT 
GCCTTCCAGT CAGCCCGATC GAGAGACTCC CGGAGAAGTG TGAGGATGGG CAGAAGCAGC 
CGCTCTGCTG ACCAGTGCAG GCAGTCTCTC CTTCTGTCTC CCGCTCTGAA CTACAAGTTC 
CCGGGTCTTT CTGGCAGTGC TGACGACACT CTCTCTCGGA AGCTGGACGA CGTGAGCCTC 
TCCGGGCAGG GAGCGGGCTT TGCTCATGAG GAATTCTGGA AAGCCAGAGT GGATCAGGAA 
GCCTCTGAAG ACCCTGAAGA ATGGGCTGAG CACGAAGATT ATATGACACA GCTCCTCCTC 
AAGTTTGGTG ATAAAAACCT CTTCCAGCAG CCGCCCAGCG AGGTGGACAT GAGAGCCAGT 
GAGCACTGGA TACCCGACAA CATGGTTACG AATAAGCCGG AGGCAAAGCA GAATCACCAG 
AGCCTTGCGA GTAAAAAGTA TCAGTCGGAT ATGTACTGGG CCCAGTCCCA AGACGGGCTG 
GGTGACTCCG CCTACGGCAG CCACCCAGGC CCTGCCAGCA GCCGAAGGCT GCAAGAGCTA 
GATCTGGACC ACGGTGCTGC GGGGTATAAC CATGACCAAA CCCAGTGGTA TGAAGACTCC 
CTGGAGTGCC TGTCTGACTT GAAACCAGAG CAGAGTGTCC GGGATTCTAT GGATTCTCTG 
GCGTTGTCCA ACATCACAGG GGCATCCGTG GACGGAGAAA GCAAGCCAAG GCCATCATTA 
TACTCTCTCC AAAACTTCGA GGAGATAGAG GCAGAAGACT GTGAGAAAAT GAGCAATATG 
GGAACTCTGA ATTCTTCCAT GCTGCACAGG AGCGCAGAGT CCTTAAAAAG TCTGAATTCA 
GAACTGTGCC CAGAAAAAAT TATACCGGAG GAAAAACCAG TGCACCTGCC GGTGCTCAGA 
AGATCCAAGT CTCAGTCCCG ACCACAGCAG GTCAAGTTTT CAGATGACGT CATCGACAAC 
GGAAGCTACG ACATCGAAAT CCGGCAGCCT CCAATGAGTG AAAGGACTCG GAGACGAGTG 
TATCACTTTG AAGAGAGGGG ATCCAGGCCT CACCACCATC GCCACCGGAG AAGCAGAAAG 
TCGCGGTCCG ACAACGCCCT GAACCTGGTC ACAGAAAGGA AGTACTCCGC CAAGGACAGA 
CTTCGGCTGT ACACCCCCGA TAACTATGAG AAATTCATAC AGAGTAAGGG CGCCCGGGAG 
CTCCAAGCAT ACATGCAGAA TGCCAACCTC TACGGCCAGT ATGCCCACAC CACATCGGAT 
TATGCCCTGC AGAACCCAGG AATGACCAGG TTCCTGGGGC TCTACGGGGA CGACGATGAC 
TCCTGGTGTT CATCCTCCAC TTCCTCCTCC GACTCAGAAG AAGAAGGCTA TTTTCTTGGA 
CAGCCAATCC CTCAGCCTCG GCCACAAAGA TTCACCTACT ATACGGATGA CCTTTCTAGT 
CCGGCTTCTG CACTGCCCAC CCCACAGTTT AATCAGAGGA CAACTAAATC CAAGAAGAAA 
AAGGGACACA GGGGCAAAAA CTGTATCATT TCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Global genetic analysis in mice unveils central role for cilia in congenital heart disease.
Nature521(7553)520-4(2015 May)
Li Y,Klena NT,Gabriel GC,Liu X,Kim AJ,Lemke K,Chen Y,Chatterjee B,Devine W,Damerla RR,Chang C,Yagi H,San Agustin JT,Thahir M,Anderton S,Lawhead C,Vescovi A,Pratt H,Morgan J,Haynes L,Smith CL,Eppig JT,Reinholdt L,Francis R,Leatherbury L,Ganapathiraju MK,Tobita K,Pazour GJ,Lo CW

PRICKLE1 interaction with SYNAPSIN I reveals a role in autism spectrum disorders.
PloS one8(12)e80737(2013)
Paemka L,Mahajan VB,Skeie JM,Sowers LP,Ehaideb SN,Gonzalez-Alegre P,Sasaoka T,Tao H,Miyagi A,Ueno N,Takao K,Miyakawa T,Wu S,Darbro BW,Ferguson PJ,Pieper AA,Britt JK,Wemmie JA,Rudd DS,Wassink T,El-Shanti H,Mefford HC,Carvill GL,Manak JR,Bassuk AG

Nuclear localization of Prickle2 is required to establish cell polarity during early mouse embryogenesis.
Developmental biology364(2)138-48(2012 Apr)
Tao H,Inoue K,Kiyonari H,Bassuk AG,Axelrod JD,Sasaki H,Aizawa S,Ueno N

Identification and characterization of novel rare mutations in the planar cell polarity gene PRICKLE1 in human neural tube defects.
Human mutation32(12)1371-5(2011 Dec)
Bosoi CM,Capra V,Allache R,Trinh VQ,De Marco P,Merello E,Drapeau P,Bassuk AG,Kibar Z

Neuron-restrictive silencer factor-mediated hyperpolarization-activated cyclic nucleotide gated channelopathy in experimental temporal lobe epilepsy.
Annals of neurology70(3)454-64(2011 Sep)
McClelland S,Flynn C,Dub? C,Richichi C,Zha Q,Ghestem A,Esclapez M,Bernard C,Baram TZ

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Prickle1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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