Fbln1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Fbln1
Entrez Gene ID	315191
Full Name	fibulin 1
General protein information	Preferred Names fibulin 1 Names fibulin-1 Fbln1 protein-like
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 7 Map Location: 7q34

mRNA and Protein(s)

mRNA	Protein	Name
NM_001127547.1	NP_001121019.1	fibulin-1 precursor
XM_006242161.2	XP_006242223.1	fibulin-1 isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	FBLN1	XP_003317358.1
Canis lupus familiaris (dog)	FBLN1	XP_005625811.1
Caenorhabditis elegans (roundworm)	fbl-1	NP_001023237.1
Homo sapiens (human)	FBLN1	NP_006477.2
	FBLN1	XP_001109966.2
Gallus gallus (chicken)	FBLN1	NP_989496.1
Bos taurus (cattle)	FBLN1	NP_001091498.1
Mus musculus (house mouse)	Fbln1	NP_034310.2
Danio rerio (zebrafish)	fbln1	NP_571117.1
Rattus norvegicus (Norway rat)	Fbln1	NP_001121019.1

Gene Ontology
Bibliography

Related articles in PubMed

Extracellular matrix remodelling in response to venous hypertension: proteomics of human varicose veins.
Barallobre-Barreiro J, Oklu R, Lynch M, Fava M, Baig F, Yin X, Barwari T, Potier DN, Albadawi H, Jahangiri M, Porter KE, Watkins MT, Misra S, Stoughton J, Mayr M
Cardiovascular research110(3)419-30(2016 06)

Fibulin1C peptide induces cell attachment and extracellular matrix deposition in lung fibroblasts.
Ge Q, Chen L, Jaffar J, Argraves WS, Twal WO, Hansbro P, Black JL, Burgess JK, Oliver B
Scientific reports59496(2015 Apr)

A novel intracellular fibulin-1D variant binds to the cytoplasmic domain of integrin beta 1 subunit.
Twal WO, Hammad SM, Guffy SL, Argraves WS
Matrix biology : journal of the International Society for Matrix Biology4397-108(2015 Apr)

Extracellular matrix secretion by cardiac fibroblasts: role of microRNA-29b and microRNA-30c.
Abonnenc M, Nabeebaccus AA, Mayr U, Barallobre-Barreiro J, Dong X, Cuello F, Sur S, Drozdov I, Langley SR, Lu R, Stathopoulou K, Didangelos A, Yin X, Zimmermann WH, Shah AM, Zampetaki A, Mayr M
Circulation research113(10)1138-47(2013 Oct)

Proteomics characterization of extracellular space components in the human aorta.
Didangelos A, Yin X, Mandal K, Baumert M, Jahangiri M, Mayr M
Molecular & cellular proteomics : MCP9(9)2048-62(2010 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

fibulin-1 and -2 respond differentially to single and repeated damaging noxae, and their expression is differently present in liver cells
Title: Expression of ECM proteins fibulin-1 and -2 in acute and chronic liver disease and in cultured rat liver cells.

The following Fbln1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Fbln1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa01765	NM_001127547.1 Latest version!	Rattus norvegicus fibulin 1 (Fbln1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$412.30 $589.00
ORa30646	XM_006242161.2 Latest version!	Rattus norvegicus fibulin 1 (Fbln1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Fbln1 ( NM_001127547.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001121019.1 Rattus norvegicus fibulin 1 (Fbln1), mRNA.

Price & Availability↑

CloneID	ORa01765
Clone ID Related Accession (Same CDS sequence)	NM_001127547.1
Accession Version	NM_001127547.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2118bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-07
Organism	Rattus norvegicus(Norway rat)
Product	fibulin-1 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CH473950.2. On or before May 23, 2008 this sequence version replaced XM_243637.4, XM_001078267.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGAGCGCC CCGCGCCGTC GCGCCTCGTC CCGCTGCCGC TGCTGCTGCT CGGCAGCCTC 
TCGCTGCTGG CGGCCCGAGC GAATGCAGAC ATCTCCATGG AGGCTTGCTG CACGGAGGGA 
AATCAAATGG CTAACCAGCA CAGGGACTGC TCGCTCTCCT ACACCTCAGA ATCCAAGGAG 
TGCAGGATGG TCCAGGAACA GTGTTGTCAC AACCAACTGG AGGAGCTGCA CTGTGCCACA 
GGCATCAACC TGGCCAGCGA ACCAGACGGC TGCGCCTCCC TCCACAACTA CAACAGTAGC 
CTCGAGACCA TCTTCATAAA GAGGTGCTGC CACTGTTGCA TGCTGGGAAG GGCAGCCCAG 
GCCCGAGGCC AGACCTGTGA GCCCAACCTC ATGATAAGCT ACCAGTGTGG ACTGGTATTC 
CGTGCCTGCT GCGTGAAGGG ACAGGAGAAT TCAGACTTTG TCAGAGGCGA TGGTACGGAC 
CTTCAGGACC CAGCTAAGAT TCCTGAGGAG GAGGAACAAG AAGACCCATA CCTGAACGAC 
CGCTGTCGAG GTGGCGGGCC CTGTAAGCAG CAGTGCCGTG ACACTGGGGA TGAGGTCATC 
TGCTCTTGCT TTGTGGGCTA CCAGCTGCAG TCGGATGGTG TCTCCTGTGA AGATATCAAT 
GAATGCATCA CCGGCAGCCA TAACTGCCGG CTGGGAGAAT CCTGCATCAA CACAGTGGGC 
TCTTTCCGCT GCCAGCGGGA CAGCAGCTGT GGGACTGGCT ATGAGCTCAC AGAGGATAAT 
AACTGCAAAG ATATTGACGA GTGTGAGACT GGTATTCATA ACTGCCCCCC CGATTTTATC 
TGTCAGAATA CTCTGGGATC CTTCCGTTGC AGACCCAAGC TGCAGTGCAA GAGCGGCTTT 
ATACAGGATG CTCTAGGCAA CTGCATTGAT ATCAATGAGT GTTTAAGTAT CAGTGCCCCA 
TGCCCTGTGG GGCAGACATG CATCAATACA GAGGGCTCCT ATACATGTCA GAAGAATGTG 
CCCAACTGTG GCCGTGGATA TCATCTCAAC GAAGAGGGGA CCCGCTGTGT TGACGTGGAT 
GAGTGCTCAC CACCAGCCGA GCCCTGTGGG AAGGGACACC ACTGTTTGAA CTCCCCCGGC 
AGCTTCCGCT GCGAGTGCAA GGCCGGGTAC TATTTTGATG GCATCAGCAG GACCTGTGTG 
GATATCAACG AGTGCCAGCG CTATCCTGGG CGCCTGTGTG GCCACAAGTG TGAGAACACG 
CCAGGCTCCT ACCACTGCAG CTGCTCTGCG GGCTTCCGGC TGTCTGTGGA CGGCCGGTCT 
TGTGAAGACG TGAACGAGTG CCTTAACAGC CCTTGCAGCC AGGAGTGTGC TAACGTCTAT 
GGCTCCTACC AGTGCTACTG CCGACGAGGT TATCAGCTCA GTGACGTGGA CGGGGTCACC 
TGCGAAGATA TTGATGAGTG TGCCCTGCCC ACGGGGGGTC ACATCTGCTC CTACCGCTGC 
ATCAACATCC CGGGAAGCTT CCAGTGCAGC TGTCCCTCAT CCGGCTACAG GCTAGCTCCC 
AATGGTCGCA ACTGCCAAGA CATTGACGAG TGTGTGACCG GTATCCACAA CTGTTCCATC 
AACGAGACCT GCTTCAACAT CCAGGGCAGC TTCCGCTGTC TATCCTTCGA GTGCCCTGAG 
AACTATCGCC GCTCTGCAGA CACCTTTCGC CAAGAGAAGA CAGACACCGT CCGCTGCATC 
AAGTCTTGCC GTCCCACCGA TGAGGCCTGC ATGCGGGACC CTGTGCATAC CGTCTCCCAC 
ACTGTCATCT CCCTGCCCAC CTTCCGAGAG TTCACCCGTC CGGAGGAGAT CATCTTTCTG 
AGGGCTGTCA CGCCGCTGTA TCCTGCCAAC CAGGCTGACA TCATCTTCGA CATCACAGAA 
GGGAACCTTC GAGACTCCTT TGATATCATC AAGCGCTATG AGGACGGCAT GACTGTGGGT 
GTCGTGCGCC AAGTGCGGCC CATCGTGGGC CCATTTTATG CCGTCCTGAA GCTGGAGATG 
AACTACGTGT TGGGAGGCGT GGTTTCCCAC CGGAACGTCG TCAATGTGCA TATCTTTGTC 
TCTGAGTACT GGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001121019.1
CDS	125..2242
Translation	MERPAPSRLVPLPLLLLGSLSLLAARANADISMEACCTEGNQMANQHRDCSLSYTSESKECRMVQEQCCHNQLEELHCATGINLASEPDGCASLHNYNSSLETIFIKRCCHCCMLGRAAQARGQTCEPNLMISYQCGLVFRACCVKGQENSDFVRGDGTDLQDPAKIPEEEEQEDPYLNDRCRGGGPCKQQCRDTGDEVICSCFVGYQLQSDGVSCEDINECITGSHNCRLGESCINTVGSFRCQRDSSCGTGYELTEDNNCKDIDECETGIHNCPPDFICQNTLGSFRCRPKLQCKSGFIQDALGNCIDINECLSISAPCPVGQTCINTEGSYTCQKNVPNCGRGYHLNEEGTRCVDVDECSPPAEPCGKGHHCLNSPGSFRCECKAGYYFDGISRTCVDINECQRYPGRLCGHKCENTPGSYHCSCSAGFRLSVDGRSCEDVNECLNSPCSQECANVYGSYQCYCRRGYQLSDVDGVTCEDIDECALPTGGHICSYRCINIPGSFQCSCPSSGYRLAPNGRNCQDIDECVTGIHNCSINETCFNIQGSFRCLSFECPENYRRSADTFRQEKTDTVRCIKSCRPTDEACMRDPVHTVSHTVISLPTFREFTRPEEIIFLRAVTPLYPANQADIIFDITEGNLRDSFDIIKRYEDGMTVGVVRQVRPIVGPFYAVLKLEMNYVLGGVVSHRNVVNVHIFVSEYWF

Target ORF information:

RefSeq Version	NM_001127547.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus fibulin 1 (Fbln1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001127547.1

ORF Insert Sequence:

ATGGAGCGCC CCGCGCCGTC GCGCCTCGTC CCGCTGCCGC TGCTGCTGCT CGGCAGCCTC 
TCGCTGCTGG CGGCCCGAGC GAATGCAGAC ATCTCCATGG AGGCTTGCTG CACGGAGGGA 
AATCAAATGG CTAACCAGCA CAGGGACTGC TCGCTCTCCT ACACCTCAGA ATCCAAGGAG 
TGCAGGATGG TCCAGGAACA GTGTTGTCAC AACCAACTGG AGGAGCTGCA CTGTGCCACA 
GGCATCAACC TGGCCAGCGA ACCAGACGGC TGCGCCTCCC TCCACAACTA CAACAGTAGC 
CTCGAGACCA TCTTCATAAA GAGGTGCTGC CACTGTTGCA TGCTGGGAAG GGCAGCCCAG 
GCCCGAGGCC AGACCTGTGA GCCCAACCTC ATGATAAGCT ACCAGTGTGG ACTGGTATTC 
CGTGCCTGCT GCGTGAAGGG ACAGGAGAAT TCAGACTTTG TCAGAGGCGA TGGTACGGAC 
CTTCAGGACC CAGCTAAGAT TCCTGAGGAG GAGGAACAAG AAGACCCATA CCTGAACGAC 
CGCTGTCGAG GTGGCGGGCC CTGTAAGCAG CAGTGCCGTG ACACTGGGGA TGAGGTCATC 
TGCTCTTGCT TTGTGGGCTA CCAGCTGCAG TCGGATGGTG TCTCCTGTGA AGATATCAAT 
GAATGCATCA CCGGCAGCCA TAACTGCCGG CTGGGAGAAT CCTGCATCAA CACAGTGGGC 
TCTTTCCGCT GCCAGCGGGA CAGCAGCTGT GGGACTGGCT ATGAGCTCAC AGAGGATAAT 
AACTGCAAAG ATATTGACGA GTGTGAGACT GGTATTCATA ACTGCCCCCC CGATTTTATC 
TGTCAGAATA CTCTGGGATC CTTCCGTTGC AGACCCAAGC TGCAGTGCAA GAGCGGCTTT 
ATACAGGATG CTCTAGGCAA CTGCATTGAT ATCAATGAGT GTTTAAGTAT CAGTGCCCCA 
TGCCCTGTGG GGCAGACATG CATCAATACA GAGGGCTCCT ATACATGTCA GAAGAATGTG 
CCCAACTGTG GCCGTGGATA TCATCTCAAC GAAGAGGGGA CCCGCTGTGT TGACGTGGAT 
GAGTGCTCAC CACCAGCCGA GCCCTGTGGG AAGGGACACC ACTGTTTGAA CTCCCCCGGC 
AGCTTCCGCT GCGAGTGCAA GGCCGGGTAC TATTTTGATG GCATCAGCAG GACCTGTGTG 
GATATCAACG AGTGCCAGCG CTATCCTGGG CGCCTGTGTG GCCACAAGTG TGAGAACACG 
CCAGGCTCCT ACCACTGCAG CTGCTCTGCG GGCTTCCGGC TGTCTGTGGA CGGCCGGTCT 
TGTGAAGACG TGAACGAGTG CCTTAACAGC CCTTGCAGCC AGGAGTGTGC TAACGTCTAT 
GGCTCCTACC AGTGCTACTG CCGACGAGGT TATCAGCTCA GTGACGTGGA CGGGGTCACC 
TGCGAAGATA TTGATGAGTG TGCCCTGCCC ACGGGGGGTC ACATCTGCTC CTACCGCTGC 
ATCAACATCC CGGGAAGCTT CCAGTGCAGC TGTCCCTCAT CCGGCTACAG GCTAGCTCCC 
AATGGTCGCA ACTGCCAAGA CATTGACGAG TGTGTGACCG GTATCCACAA CTGTTCCATC 
AACGAGACCT GCTTCAACAT CCAGGGCAGC TTCCGCTGTC TATCCTTCGA GTGCCCTGAG 
AACTATCGCC GCTCTGCAGA CACCTTTCGC CAAGAGAAGA CAGACACCGT CCGCTGCATC 
AAGTCTTGCC GTCCCACCGA TGAGGCCTGC ATGCGGGACC CTGTGCATAC CGTCTCCCAC 
ACTGTCATCT CCCTGCCCAC CTTCCGAGAG TTCACCCGTC CGGAGGAGAT CATCTTTCTG 
AGGGCTGTCA CGCCGCTGTA TCCTGCCAAC CAGGCTGACA TCATCTTCGA CATCACAGAA 
GGGAACCTTC GAGACTCCTT TGATATCATC AAGCGCTATG AGGACGGCAT GACTGTGGGT 
GTCGTGCGCC AAGTGCGGCC CATCGTGGGC CCATTTTATG CCGTCCTGAA GCTGGAGATG 
AACTACGTGT TGGGAGGCGT GGTTTCCCAC CGGAACGTCG TCAATGTGCA TATCTTTGTC 
TCTGAGTACT GGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Fbln1 ( XM_006242161.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006242223.1 Rattus norvegicus fibulin 1 (Fbln1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa30646
Clone ID Related Accession (Same CDS sequence)	XM_006242161.2
Accession Version	XM_006242161.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2058bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	fibulin-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005106.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 7, 2014 this sequence version replaced XM_006242161.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCGCC CCGCGCCGTC GCGCCTCGTC CCGCTGCCGC TGCTGCTGCT CGGCAGCCTC 
TCGCTGCTGG CGGCCCGAGC GAATGCAGAC ATCTCCATGG AGGCTTGCTG CACGGAGGGA 
AATCAAATGG CTAACCAGCA CAGGGACTGC TCGCTCTCCT ACACCTCAGA ATCCAAGGAG 
TGCAGGATGG TCCAGGAACA GTGTTGTCAC AACCAACTGG AGGAGCTGCA CTGTGCCACA 
GGCATCAACC TGGCCAGCGA ACCAGACGGC TGCGCCTCCC TCCACAACTA CAACAGTAGC 
CTCGAGACCA TCTTCATAAA GAGGTGCTGC CACTGTTGCA TGCTGGGAAG GGCAGCCCAG 
GCCCGAGGCC AGACCTGTGA GCCCAACCTC ATGATAAGCT ACCAGTGTGG ACTGGTATTC 
CGTGCCTGCT GCGTGAAGGG ACAGGAGAAT TCAGACTTTG TCAGAGGCGA TGGTACGGAC 
CTTCAGGACC CAGCTAAGAT TCCTGAGGAG GAGGAACAAG AAGACCCATA CCTGAACGAC 
CGCTGTCGAG GTGGCGGGCC CTGTAAGCAG CAGTGCCGTG ACACTGGGGA TGAGGTCATC 
TGCTCTTGCT TTGTGGGCTA CCAGCTGCAG TCGGATGGTG TCTCCTGTGA AGATATCAAT 
GAATGCATCA CCGGCAGCCA TAACTGCCGG CTGGGAGAAT CCTGCATCAA CACAGTGGGC 
TCTTTCCGCT GCCAGCGGGA CAGCAGCTGT GGGACTGGCT ATGAGCTCAC AGAGGATAAT 
AACTGCAAAG ATATTGACGA GTGTGAGACT GGTATTCATA ACTGCCCCCC CGATTTTATC 
TGTCAGAATA CTCTGGGATC CTTCCGTTGC AGACCCAAGC TGCAGTGCAA GAGCGGCTTT 
ATACAGGATG CTCTAGGCAA CTGCATTGAT ATCAATGAGT GTTTAAGTAT CAGTGCCCCA 
TGCCCTGTGG GGCAGACATG CATCAATACA GAGGGCTCCT ATACATGTCA GAAGAATGTG 
CCCAACTGTG GCCGTGGATA TCATCTCAAC GAAGAGGGGA CCCGCTGTGT TGACGTGGAT 
GAGTGCTCAC CACCAGCCGA GCCCTGTGGG AAGGGACACC ACTGTTTGAA CTCCCCCGGC 
AGCTTCCGCT GCGAGTGCAA GGCCGGGTAC TATTTTGATG GCATCAGCAG GACCTGTGTG 
GATATCAACG AGTGCCAGCG CTATCCTGGG CGCCTGTGTG GCCACAAGTG TGAGAACACG 
CCAGGCTCCT ACCACTGCAG CTGCTCTGCG GGCTTCCGGC TGTCTGTGGA CGGCCGGTCT 
TGTGAAGACG TGAACGAGTG CCTTAACAGC CCTTGCAGCC AGGAGTGTGC TAACGTCTAT 
GGCTCCTACC AGTGCTACTG CCGACGAGGT TATCAGCTCA GTGACGTGGA CGGGGTCACC 
TGCGAAGATA TTGATGAGTG TGCCCTGCCC ACGGGGGGTC ACATCTGCTC CTACCGCTGC 
ATCAACATCC CGGGAAGCTT CCAGTGCAGC TGTCCCTCAT CCGGCTACAG GCTAGCTCCC 
AATGGTCGCA ACTGCCAAGA CATTGACGAG TGTGTGACCG GTATCCACAA CTGTTCCATC 
AACGAGACCT GCTTCAACAT CCAGGGCAGC TTCCGCTGTC TATCCTTCGA GTGCCCTGAG 
AACTATCGCC GCTCTGCAGA CACCCGCTGT GAGCGCTTGC CTTGCCATGA GAACCAGGAG 
TGCCCCAGGC TGCCTCTGAG AATAACCTAC TACCACCTCT CTTTCCCCAC CAACATCCAA 
GTGCCCGCGG TGGTTTTCCG CATGGGCCCT TCCAGTGCTG TCCCTGGGGA CAGCATGCAG 
CTGGCCATCA CCGCTGGCAA TGAGGAGGGC TTTTTCACCA CCCGAAAGGT GAGCCACCAC 
AGTGGGGTGG TGGCCCTCAC CAGGCCCATC CCTGAGCCCA GGGACTTGCT CCTGACCGTC 
ACGATGGATC TTTATCGCCA CGGCACAGTC AGCTCCTTTG TGGCCAAGCT TTATATTTTC 
GTGTTTGCAG AGCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006242223.1
CDS	105..2162
Translation	MERPAPSRLVPLPLLLLGSLSLLAARANADISMEACCTEGNQMANQHRDCSLSYTSESKECRMVQEQCCHNQLEELHCATGINLASEPDGCASLHNYNSSLETIFIKRCCHCCMLGRAAQARGQTCEPNLMISYQCGLVFRACCVKGQENSDFVRGDGTDLQDPAKIPEEEEQEDPYLNDRCRGGGPCKQQCRDTGDEVICSCFVGYQLQSDGVSCEDINECITGSHNCRLGESCINTVGSFRCQRDSSCGTGYELTEDNNCKDIDECETGIHNCPPDFICQNTLGSFRCRPKLQCKSGFIQDALGNCIDINECLSISAPCPVGQTCINTEGSYTCQKNVPNCGRGYHLNEEGTRCVDVDECSPPAEPCGKGHHCLNSPGSFRCECKAGYYFDGISRTCVDINECQRYPGRLCGHKCENTPGSYHCSCSAGFRLSVDGRSCEDVNECLNSPCSQECANVYGSYQCYCRRGYQLSDVDGVTCEDIDECALPTGGHICSYRCINIPGSFQCSCPSSGYRLAPNGRNCQDIDECVTGIHNCSINETCFNIQGSFRCLSFECPENYRRSADTRCERLPCHENQECPRLPLRITYYHLSFPTNIQVPAVVFRMGPSSAVPGDSMQLAITAGNEEGFFTTRKVSHHSGVVALTRPIPEPRDLLLTVTMDLYRHGTVSSFVAKLYIFVFAEL

Target ORF information:

RefSeq Version	XM_006242161.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus fibulin 1 (Fbln1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006242161.2

ORF Insert Sequence:

ATGGAGCGCC CCGCGCCGTC GCGCCTCGTC CCGCTGCCGC TGCTGCTGCT CGGCAGCCTC 
TCGCTGCTGG CGGCCCGAGC GAATGCAGAC ATCTCCATGG AGGCTTGCTG CACGGAGGGA 
AATCAAATGG CTAACCAGCA CAGGGACTGC TCGCTCTCCT ACACCTCAGA ATCCAAGGAG 
TGCAGGATGG TCCAGGAACA GTGTTGTCAC AACCAACTGG AGGAGCTGCA CTGTGCCACA 
GGCATCAACC TGGCCAGCGA ACCAGACGGC TGCGCCTCCC TCCACAACTA CAACAGTAGC 
CTCGAGACCA TCTTCATAAA GAGGTGCTGC CACTGTTGCA TGCTGGGAAG GGCAGCCCAG 
GCCCGAGGCC AGACCTGTGA GCCCAACCTC ATGATAAGCT ACCAGTGTGG ACTGGTATTC 
CGTGCCTGCT GCGTGAAGGG ACAGGAGAAT TCAGACTTTG TCAGAGGCGA TGGTACGGAC 
CTTCAGGACC CAGCTAAGAT TCCTGAGGAG GAGGAACAAG AAGACCCATA CCTGAACGAC 
CGCTGTCGAG GTGGCGGGCC CTGTAAGCAG CAGTGCCGTG ACACTGGGGA TGAGGTCATC 
TGCTCTTGCT TTGTGGGCTA CCAGCTGCAG TCGGATGGTG TCTCCTGTGA AGATATCAAT 
GAATGCATCA CCGGCAGCCA TAACTGCCGG CTGGGAGAAT CCTGCATCAA CACAGTGGGC 
TCTTTCCGCT GCCAGCGGGA CAGCAGCTGT GGGACTGGCT ATGAGCTCAC AGAGGATAAT 
AACTGCAAAG ATATTGACGA GTGTGAGACT GGTATTCATA ACTGCCCCCC CGATTTTATC 
TGTCAGAATA CTCTGGGATC CTTCCGTTGC AGACCCAAGC TGCAGTGCAA GAGCGGCTTT 
ATACAGGATG CTCTAGGCAA CTGCATTGAT ATCAATGAGT GTTTAAGTAT CAGTGCCCCA 
TGCCCTGTGG GGCAGACATG CATCAATACA GAGGGCTCCT ATACATGTCA GAAGAATGTG 
CCCAACTGTG GCCGTGGATA TCATCTCAAC GAAGAGGGGA CCCGCTGTGT TGACGTGGAT 
GAGTGCTCAC CACCAGCCGA GCCCTGTGGG AAGGGACACC ACTGTTTGAA CTCCCCCGGC 
AGCTTCCGCT GCGAGTGCAA GGCCGGGTAC TATTTTGATG GCATCAGCAG GACCTGTGTG 
GATATCAACG AGTGCCAGCG CTATCCTGGG CGCCTGTGTG GCCACAAGTG TGAGAACACG 
CCAGGCTCCT ACCACTGCAG CTGCTCTGCG GGCTTCCGGC TGTCTGTGGA CGGCCGGTCT 
TGTGAAGACG TGAACGAGTG CCTTAACAGC CCTTGCAGCC AGGAGTGTGC TAACGTCTAT 
GGCTCCTACC AGTGCTACTG CCGACGAGGT TATCAGCTCA GTGACGTGGA CGGGGTCACC 
TGCGAAGATA TTGATGAGTG TGCCCTGCCC ACGGGGGGTC ACATCTGCTC CTACCGCTGC 
ATCAACATCC CGGGAAGCTT CCAGTGCAGC TGTCCCTCAT CCGGCTACAG GCTAGCTCCC 
AATGGTCGCA ACTGCCAAGA CATTGACGAG TGTGTGACCG GTATCCACAA CTGTTCCATC 
AACGAGACCT GCTTCAACAT CCAGGGCAGC TTCCGCTGTC TATCCTTCGA GTGCCCTGAG 
AACTATCGCC GCTCTGCAGA CACCCGCTGT GAGCGCTTGC CTTGCCATGA GAACCAGGAG 
TGCCCCAGGC TGCCTCTGAG AATAACCTAC TACCACCTCT CTTTCCCCAC CAACATCCAA 
GTGCCCGCGG TGGTTTTCCG CATGGGCCCT TCCAGTGCTG TCCCTGGGGA CAGCATGCAG 
CTGGCCATCA CCGCTGGCAA TGAGGAGGGC TTTTTCACCA CCCGAAAGGT GAGCCACCAC 
AGTGGGGTGG TGGCCCTCAC CAGGCCCATC CCTGAGCCCA GGGACTTGCT CCTGACCGTC 
ACGATGGATC TTTATCGCCA CGGCACAGTC AGCTCCTTTG TGGCCAAGCT TTATATTTTC 
GTGTTTGCAG AGCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Extracellular matrix remodelling in response to venous hypertension: proteomics of human varicose veins.
Cardiovascular research110(3)419-30(2016 06)
Barallobre-Barreiro J,Oklu R,Lynch M,Fava M,Baig F,Yin X,Barwari T,Potier DN,Albadawi H,Jahangiri M,Porter KE,Watkins MT,Misra S,Stoughton J,Mayr M

Fibulin1C peptide induces cell attachment and extracellular matrix deposition in lung fibroblasts.
Scientific reports59496(2015 Apr)
Ge Q,Chen L,Jaffar J,Argraves WS,Twal WO,Hansbro P,Black JL,Burgess JK,Oliver B

A novel intracellular fibulin-1D variant binds to the cytoplasmic domain of integrin beta 1 subunit.
Matrix biology : journal of the International Society for Matrix Biology4397-108(2015 Apr)
Twal WO,Hammad SM,Guffy SL,Argraves WS

Extracellular matrix secretion by cardiac fibroblasts: role of microRNA-29b and microRNA-30c.
Circulation research113(10)1138-47(2013 Oct)
Abonnenc M,Nabeebaccus AA,Mayr U,Barallobre-Barreiro J,Dong X,Cuello F,Sur S,Drozdov I,Langley SR,Lu R,Stathopoulou K,Didangelos A,Yin X,Zimmermann WH,Shah AM,Zampetaki A,Mayr M

Proteomics characterization of extracellular space components in the human aorta.
Molecular & cellular proteomics : MCP9(9)2048-62(2010 Sep)
Didangelos A,Yin X,Mandal K,Baumert M,Jahangiri M,Mayr M

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