Ric8b cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ric8b
Entrez Gene ID	314681
Full Name	RIC8 guanine nucleotide exchange factor B
General protein information	Preferred Names RIC8 guanine nucleotide exchange factor B Names synembryn-B heterotrimeric G protein guanine nucleotide exchange factor-like protein Ric-8B resistance to inhibitors of cholinesterase 8 homolog B resistance to inhibitors of cholinesterase 8B
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 7 Map Location: 7q13
Summary	binds heterotrimeric G proteins; may act as a nonreceptor guanine nucleotide exchange factor [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_008765236.2	XP_008763458.1	synembryn-B isoform X4
XM_008765237.2	XP_008763459.1	synembryn-B isoform X5
XM_017594824.1	XP_017450313.1	synembryn-B isoform X2
XM_006241182.3	XP_006241244.1	synembryn-B isoform X3
XM_006241180.3	XP_006241242.1	synembryn-B isoform X1
NM_175598.2	NP_783188.1	synembryn-B
XM_008765234.2	XP_008763456.1	synembryn-B isoform X2

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	RIC8B	XP_003952332.1
Mus musculus (house mouse)	Ric8b	NP_898995.1
Homo sapiens (human)	RIC8B	NP_060627.2
Bos taurus (cattle)	RIC8B	XP_002687709.2
Rattus norvegicus (Norway rat)	Ric8b	NP_783188.1
Gallus gallus (chicken)	RIC8B	XP_416305.3
Danio rerio (zebrafish)	ric8b	NP_001005293.1
Canis lupus familiaris (dog)	RIC8B	XP_005626003.1
Xenopus tropicalis (tropical clawed frog)	ric8b	XP_002942248.2

Gene Ontology
Bibliography

Related articles in PubMed

ARMS/Kidins220 and synembryn-B levels regulate NGF-mediated secretion.
L?pez-Benito S, Lillo C, Hern?ndez-Hern?ndez ?, Chao MV, Ar?valo JC
Journal of cell science129(9)1866-77(2016 05)

A functional N-terminal domain in C/EBP?-LAP* is required for interacting with SWI/SNF and to repress Ric-8B gene transcription in osteoblasts.
Aguilar R, Grandy R, Meza D, Sepulveda H, Pihan P, van Wijnen AJ, Lian JB, Stein GS, Stein JL, Montecino M
Journal of cellular physiology229(10)1521-8(2014 Oct)

Ric-8B is a GTP-dependent G protein alphas guanine nucleotide exchange factor.
Chan P, Gabay M, Wright FA, Tall GG
The Journal of biological chemistry286(22)19932-42(2011 Jun)

Human brain synembryn interacts with Gsalpha and Gqalpha and is translocated to the plasma membrane in response to isoproterenol and carbachol.
Klattenhoff C, Montecino M, Soto X, Guzm?n L, Romo X, Garc?a MA, Mellstrom B, Naranjo JR, Hinrichs MV, Olate J
Journal of cellular physiology195(2)151-7(2003 May)

Mammalian Ric-8A (synembryn) is a heterotrimeric Galpha protein guanine nucleotide exchange factor.
Tall GG, Krumins AM, Gilman AG
The Journal of biological chemistry278(10)8356-62(2003 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

overexpression of ARMS blocked NGF-mediated secretion, without affecting basal secretion, a decrease in ARMS resulted in potentiation. Similar effects were observed with synembryn-B, a protein that interacts directly with ARMS. Downstream of ARMS and synembryn-B are Galphaq and Trio proteins, which modulate the activity of Rac1 in response to NGF
Title: ARMS/Kidins220 and synembryn-B levels regulate NGF-mediated secretion.

Results indicate that a functional N-terminus in C/EBPbeta-LAP* is required for interacting with SWI/SNF and for Ric-8B gene repression in osteoblasts.
Title: A functional N-terminal domain in C/EBP?-LAP* is required for interacting with SWI/SNF and to repress Ric-8B gene transcription in osteoblasts.

Ric-8BFL-catalyzed nucleotide exchange probably proceeds in the forward direction to produce Galpha-GTP in cells.
Title: Ric-8B is a GTP-dependent G protein alphas guanine nucleotide exchange factor.

two mammalian homologs of the Caenorhabditis elegans protein Ric-8 were identified: Ric-8A (Ric-8/synembryn) and Ric-8B
Title: Mammalian Ric-8A (synembryn) is a heterotrimeric Galpha protein guanine nucleotide exchange factor.

The following Ric8b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ric8b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa03873	NM_175598.2 Latest version!	Rattus norvegicus RIC8 guanine nucleotide exchange factor B (Ric8b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
ORa30021	XM_006241180.3 Latest version!	Rattus norvegicus RIC8 guanine nucleotide exchange factor B (Ric8b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
ORa30022	XM_008765234.2 Latest version!	Rattus norvegicus RIC8 guanine nucleotide exchange factor B (Ric8b), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
ORa30022	XM_017594824.1 Latest version!	Rattus norvegicus RIC8 guanine nucleotide exchange factor B (Ric8b), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
ORa30023	XM_006241182.3 Latest version!	Rattus norvegicus RIC8 guanine nucleotide exchange factor B (Ric8b), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
ORa47351	XM_008765236.2 Latest version!	Rattus norvegicus RIC8 guanine nucleotide exchange factor B (Ric8b), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
ORa47352	XM_008765237.2 Latest version!	Rattus norvegicus RIC8 guanine nucleotide exchange factor B (Ric8b), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ric8b ( NM_175598.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_783188.1 Rattus norvegicus RIC8 guanine nucleotide exchange factor B (Ric8b), mRNA.

Price & Availability↑

CloneID	ORa03873
Clone ID Related Accession (Same CDS sequence)	NM_175598.2
Accession Version	NM_175598.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1563bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-15
Organism	Rattus norvegicus(Norway rat)
Product	synembryn-B
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AY177755.1. On Jun 3, 2003 this sequence version replaced NM_175598.1. ##Evidence-Data-START## Transcript exon combination :: AY177755.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGATGAAG AGCGCGCCCT CTACATCGTC CGGGCCGGCG AGGCGGGGGC TATCGAGCGG 
GTCCTGAGAG ATTACAGCGA CAAGCATAGG GCTACTTTCA AATTTGAATC AGCAGATGAA 
GATAAAAGAA AGAAACTCTG TGAAGGCATA TTTAAGGTCC TGGTAAAGGG TGTCCCAACA 
ACATGCCAAG TGCCCTGCCT GGAAGTTCTC CGCATTCTCT CCAGAGACAA AAAGATTTTA 
GCTCCTGTAA CAACTGAGGA AAATATGCAA ATACTGCTGA GACTGGCCAA GCTCCATGAG 
TCGGACGATT CTTTGGAGAA GGTGTCAGAG TTCCCGGTCA TTGTGGAGTC ATTGAAATGT 
CTTTGTAACA TCGTGTTCAA CAGTCAGGTG GCACAGCAGC TTAGCCTGGA ACTGAATCTC 
GCTGCAAAGC TCTGTAACCT CCTGAGGAAG TGCAAGGACC GGAAATTTAT CAATGACATA 
AAGTGCTTTG ACCTGCGCTT GCTCTTTGTC CTGTCACTTT TGCACACTGA CATCAGGTCA 
CAATTGCGCT ATGAGCTCCA GGGACTGCCG CTGCTAACCC AGATCTTGGA AAGTGCCTTT 
AGCATCAAGT GGACCGATGA GTATGAGTCG GCCATAGACC ATAGTGGACC TCCTCTCTCA 
CCTCAGGAGA CAGACTGTGC CATTGAGGCC CTCAAAGCTC TGTTCAATGT GACGGTAGAC 
AGTTGGAAGG TGCATAAAGA GAGCGATTCT CATCAGTTCC GTGTCATGGC CGCTGTCCTT 
CGCCACTGTT TACTAATTGT AGGTCCAACC GAAGACAAAA CGGAAGAGCT GCACAGTAAT 
GCCGTCAACC TTTTAAGCAA TGTTCCAGTC TCTTGTTTGG ATGTTCTCAT TTGTCCATTA 
ACCCATGAAG AAACAGCCCA GGAGGCAGCA ACTCTAGACG AACTGCCCGG TGATAAGACA 
GCAGAGAAAG ACACGGCTTT GAAGAGCAGT GCCATGGTAT ACAATGGCAT GAACATGGAG 
GCCATCCACG TTTTACTCAG TTTTATGGAG AAGAGGATTG ATAAGGGAAG CAGCTATAGA 
GAGGGTCTAA CTCCAGTTCT CAGCTTATTA ACAGAATGTT CCCGAGCCCA TCGGAACATC 
CGAAAATTTC TCAAAGATCA GGTGTTACCA CCTTTGAGGG ATGTGACAAA TCGACCAGAA 
GTCGGCTCAA CCGTGAGAAA TAAGCTGGTG CGCCTCATGA CACACGTTGA CCTCGGAGTC 
AAGCAGATTG CTGCTGAGTT CCTCTTCGTC CTTTGCAAAG AGCGAGTGGA TAGTCTGCTG 
AAGTACACTG GCTATGGGAA CGCTGCAGGA CTGCTGGCTG CCAGGGGCCT CTTGGCTGGA 
GGAAGAGGAG ACAATTGGTA CTCAGAGGAC GAGGACACGG ACACTGAGGA ATACAAAAAC 
GCAAAACCAA AAGAGGAGTT GCTTAAACCA ATGGGACTAA AACCTGACGG GACAATAACG 
CCTTTGGAGG AAGCGCTCAG CCAGTATTCT GTCATCGAAG AGACCAGCTC GGACACAGAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_783188.1
CDS	88..1650
Translation	MDEERALYIVRAGEAGAIERVLRDYSDKHRATFKFESADEDKRKKLCEGIFKVLVKGVPTTCQVPCLEVLRILSRDKKILAPVTTEENMQILLRLAKLHESDDSLEKVSEFPVIVESLKCLCNIVFNSQVAQQLSLELNLAAKLCNLLRKCKDRKFINDIKCFDLRLLFVLSLLHTDIRSQLRYELQGLPLLTQILESAFSIKWTDEYESAIDHSGPPLSPQETDCAIEALKALFNVTVDSWKVHKESDSHQFRVMAAVLRHCLLIVGPTEDKTEELHSNAVNLLSNVPVSCLDVLICPLTHEETAQEAATLDELPGDKTAEKDTALKSSAMVYNGMNMEAIHVLLSFMEKRIDKGSSYREGLTPVLSLLTECSRAHRNIRKFLKDQVLPPLRDVTNRPEVGSTVRNKLVRLMTHVDLGVKQIAAEFLFVLCKERVDSLLKYTGYGNAAGLLAARGLLAGGRGDNWYSEDEDTDTEEYKNAKPKEELLKPMGLKPDGTITPLEEALSQYSVIEETSSDTD

Target ORF information:

RefSeq Version	NM_175598.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus RIC8 guanine nucleotide exchange factor B (Ric8b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_175598.2

ORF Insert Sequence:

ATGGATGAAG AGCGCGCCCT CTACATCGTC CGGGCCGGCG AGGCGGGGGC TATCGAGCGG 
GTCCTGAGAG ATTACAGCGA CAAGCATAGG GCTACTTTCA AATTTGAATC AGCAGATGAA 
GATAAAAGAA AGAAACTCTG TGAAGGCATA TTTAAGGTCC TGGTAAAGGG TGTCCCAACA 
ACATGCCAAG TGCCCTGCCT GGAAGTTCTC CGCATTCTCT CCAGAGACAA AAAGATTTTA 
GCTCCTGTAA CAACTGAGGA AAATATGCAA ATACTGCTGA GACTGGCCAA GCTCCATGAG 
TCGGACGATT CTTTGGAGAA GGTGTCAGAG TTCCCGGTCA TTGTGGAGTC ATTGAAATGT 
CTTTGTAACA TCGTGTTCAA CAGTCAGGTG GCACAGCAGC TTAGCCTGGA ACTGAATCTC 
GCTGCAAAGC TCTGTAACCT CCTGAGGAAG TGCAAGGACC GGAAATTTAT CAATGACATA 
AAGTGCTTTG ACCTGCGCTT GCTCTTTGTC CTGTCACTTT TGCACACTGA CATCAGGTCA 
CAATTGCGCT ATGAGCTCCA GGGACTGCCG CTGCTAACCC AGATCTTGGA AAGTGCCTTT 
AGCATCAAGT GGACCGATGA GTATGAGTCG GCCATAGACC ATAGTGGACC TCCTCTCTCA 
CCTCAGGAGA CAGACTGTGC CATTGAGGCC CTCAAAGCTC TGTTCAATGT GACGGTAGAC 
AGTTGGAAGG TGCATAAAGA GAGCGATTCT CATCAGTTCC GTGTCATGGC CGCTGTCCTT 
CGCCACTGTT TACTAATTGT AGGTCCAACC GAAGACAAAA CGGAAGAGCT GCACAGTAAT 
GCCGTCAACC TTTTAAGCAA TGTTCCAGTC TCTTGTTTGG ATGTTCTCAT TTGTCCATTA 
ACCCATGAAG AAACAGCCCA GGAGGCAGCA ACTCTAGACG AACTGCCCGG TGATAAGACA 
GCAGAGAAAG ACACGGCTTT GAAGAGCAGT GCCATGGTAT ACAATGGCAT GAACATGGAG 
GCCATCCACG TTTTACTCAG TTTTATGGAG AAGAGGATTG ATAAGGGAAG CAGCTATAGA 
GAGGGTCTAA CTCCAGTTCT CAGCTTATTA ACAGAATGTT CCCGAGCCCA TCGGAACATC 
CGAAAATTTC TCAAAGATCA GGTGTTACCA CCTTTGAGGG ATGTGACAAA TCGACCAGAA 
GTCGGCTCAA CCGTGAGAAA TAAGCTGGTG CGCCTCATGA CACACGTTGA CCTCGGAGTC 
AAGCAGATTG CTGCTGAGTT CCTCTTCGTC CTTTGCAAAG AGCGAGTGGA TAGTCTGCTG 
AAGTACACTG GCTATGGGAA CGCTGCAGGA CTGCTGGCTG CCAGGGGCCT CTTGGCTGGA 
GGAAGAGGAG ACAATTGGTA CTCAGAGGAC GAGGACACGG ACACTGAGGA ATACAAAAAC 
GCAAAACCAA AAGAGGAGTT GCTTAAACCA ATGGGACTAA AACCTGACGG GACAATAACG 
CCTTTGGAGG AAGCGCTCAG CCAGTATTCT GTCATCGAAG AGACCAGCTC GGACACAGAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ric8b ( XM_006241180.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006241242.1 Rattus norvegicus RIC8 guanine nucleotide exchange factor B (Ric8b), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa30021
Clone ID Related Accession (Same CDS sequence)	XM_006241180.3
Accession Version	XM_006241180.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1683bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	synembryn-B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005106.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006241180.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAAG AGCGCGCCCT CTACATCGTC CGGGCCGGCG AGGCGGGGGC TATCGAGCGG 
GTCCTGAGAG ATTACAGCGA CAAGCATAGG GCTACTTTCA AATTTGAATC AGCAGATGAA 
GATAAAAGAA AGAAACTCTG TGAAGGCATA TTTAAGGTCC TGGTAAAGGG TGTCCCAACA 
ACATGCCAAG TGCCCTGCCT GGAAGTTCTC CGCATTCTCT CCAGAGACAA AAAGATTTTA 
GCTCCTGTAA CAACTGAGGA AAATATGCAA ATACTGCTGA GACTGGCCAA GCTCCATGAG 
TCGGACGATT CTTTGGAGAA GGTGTCAGAG TTCCCGGTCA TTGTGGAGTC ATTGAAATGT 
CTTTGTAACA TCGTGTTCAA CAGTCAGGTG GCACAGCAGC TTAGCCTGGA ACTGAATCTC 
GCTGCAAAGC TCTGTAACCT CCTGAGGAAG TGCAAGGACC GAAAATTTAT CAATGACATA 
AAGTGCTTTG ACCTGCGCTT GCTCTTTGTC CTGTCACTTT TGCACACTGA CATCAGGTCA 
CAATTGCGCT ATGAGCTCCA GGGACTGCCG CTGCTAACCC AGATCTTGGA AAGTGCCTTT 
AGCATCAAGT GGACCGATGA GTATGAGTCG GCCATAGACC ATAGTGGACC TCCTCTCTCA 
CCTCAGGAGA CAGACTGTGC CATTGAGGCC CTCAAAGCTC TGTTCAATGT GACGGTAGAC 
AGTTGGAAGG TGCATAAAGA GAGCGATTCT CATCAGTTCC GTGTCATGGC CGCTGTCCTT 
CGCCACTGTT TACTAATTGT AGGTCCAACC GAAGACAAAA CGGAAGAGCT GCACAGTAAT 
GCCGTCAACC TTTTAAGCAA TGTTCCAGTC TCTTGTTTGG ATGTTCTCAT TTGTCCATTA 
ACCCATGAAG AAACAGCCCA GGAGGCAGCA ACTCTAGACG AACTGCCCGG TGATAAGACA 
GCAGAGAAAG ACACGGCTTT GAAGAGCAGT GCCATGGTAT ACAATGGCAT GAACATGGAG 
GCCATCCACG TTTTACTCAG TTTTATGGAG AAGAGGATTG ATAAGGGAAG CAGCTATAGA 
GAGGGTCTAA CTCCAGTTCT CAGCTTATTA ACAGAATGTT CCCGAGCCCA TCGGAACATC 
CGAAAATTTC TCAAAGATCA GGTGTTACCA CCTTTGAGGG ATGTGACAAA TCGACCAGAA 
GTCGGCTCAA CCGTGAGAAA TAAGCTGGTG CGCCTCATGA CACACGTTGA CCTCGGAGTC 
AAGCAGATTG CTGCTGAGTT CCTCTTCGTC CTTTGCAAAG AGCGAGTGGA TAGTCTGCTG 
AAGTACACTG GCTATGGGAA CGCTGCAGGA CTGCTGGCTG CCAGGGGCCT CTTGGCTGGA 
GGAAGAGGAG ACAATTGGTA CTCAGAGGAC GAGGACACGG ACACTGAGGA ATACAAAAAC 
GCAAAACCAA ACATTAATCT TATCACTGGT CATTTAGAGG AACCAATGCC AAACCCTATA 
GATGAAATGA CAGAAGAACA AAAAGAATAT GAAGCCATGA AACTTGTCAA CATGCTTGAT 
AAACTCTCCA GAGAGGAGTT GCTTAAACCA ATGGGACTAA AACCTGACGG GACAATAACG 
CCTTTGGAGG AAGCGCTCAG CCAGTATTCT GTCATCGAAG AGACCAGCTC GGACACAGAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006241242.1
CDS	687..2369
Translation	MDEERALYIVRAGEAGAIERVLRDYSDKHRATFKFESADEDKRKKLCEGIFKVLVKGVPTTCQVPCLEVLRILSRDKKILAPVTTEENMQILLRLAKLHESDDSLEKVSEFPVIVESLKCLCNIVFNSQVAQQLSLELNLAAKLCNLLRKCKDRKFINDIKCFDLRLLFVLSLLHTDIRSQLRYELQGLPLLTQILESAFSIKWTDEYESAIDHSGPPLSPQETDCAIEALKALFNVTVDSWKVHKESDSHQFRVMAAVLRHCLLIVGPTEDKTEELHSNAVNLLSNVPVSCLDVLICPLTHEETAQEAATLDELPGDKTAEKDTALKSSAMVYNGMNMEAIHVLLSFMEKRIDKGSSYREGLTPVLSLLTECSRAHRNIRKFLKDQVLPPLRDVTNRPEVGSTVRNKLVRLMTHVDLGVKQIAAEFLFVLCKERVDSLLKYTGYGNAAGLLAARGLLAGGRGDNWYSEDEDTDTEEYKNAKPNINLITGHLEEPMPNPIDEMTEEQKEYEAMKLVNMLDKLSREELLKPMGLKPDGTITPLEEALSQYSVIEETSSDTD

Target ORF information:

RefSeq Version	XM_006241180.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus RIC8 guanine nucleotide exchange factor B (Ric8b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006241180.3

ORF Insert Sequence:

ATGGATGAAG AGCGCGCCCT CTACATCGTC CGGGCCGGCG AGGCGGGGGC TATCGAGCGG 
GTCCTGAGAG ATTACAGCGA CAAGCATAGG GCTACTTTCA AATTTGAATC AGCAGATGAA 
GATAAAAGAA AGAAACTCTG TGAAGGCATA TTTAAGGTCC TGGTAAAGGG TGTCCCAACA 
ACATGCCAAG TGCCCTGCCT GGAAGTTCTC CGCATTCTCT CCAGAGACAA AAAGATTTTA 
GCTCCTGTAA CAACTGAGGA AAATATGCAA ATACTGCTGA GACTGGCCAA GCTCCATGAG 
TCGGACGATT CTTTGGAGAA GGTGTCAGAG TTCCCGGTCA TTGTGGAGTC ATTGAAATGT 
CTTTGTAACA TCGTGTTCAA CAGTCAGGTG GCACAGCAGC TTAGCCTGGA ACTGAATCTC 
GCTGCAAAGC TCTGTAACCT CCTGAGGAAG TGCAAGGACC GAAAATTTAT CAATGACATA 
AAGTGCTTTG ACCTGCGCTT GCTCTTTGTC CTGTCACTTT TGCACACTGA CATCAGGTCA 
CAATTGCGCT ATGAGCTCCA GGGACTGCCG CTGCTAACCC AGATCTTGGA AAGTGCCTTT 
AGCATCAAGT GGACCGATGA GTATGAGTCG GCCATAGACC ATAGTGGACC TCCTCTCTCA 
CCTCAGGAGA CAGACTGTGC CATTGAGGCC CTCAAAGCTC TGTTCAATGT GACGGTAGAC 
AGTTGGAAGG TGCATAAAGA GAGCGATTCT CATCAGTTCC GTGTCATGGC CGCTGTCCTT 
CGCCACTGTT TACTAATTGT AGGTCCAACC GAAGACAAAA CGGAAGAGCT GCACAGTAAT 
GCCGTCAACC TTTTAAGCAA TGTTCCAGTC TCTTGTTTGG ATGTTCTCAT TTGTCCATTA 
ACCCATGAAG AAACAGCCCA GGAGGCAGCA ACTCTAGACG AACTGCCCGG TGATAAGACA 
GCAGAGAAAG ACACGGCTTT GAAGAGCAGT GCCATGGTAT ACAATGGCAT GAACATGGAG 
GCCATCCACG TTTTACTCAG TTTTATGGAG AAGAGGATTG ATAAGGGAAG CAGCTATAGA 
GAGGGTCTAA CTCCAGTTCT CAGCTTATTA ACAGAATGTT CCCGAGCCCA TCGGAACATC 
CGAAAATTTC TCAAAGATCA GGTGTTACCA CCTTTGAGGG ATGTGACAAA TCGACCAGAA 
GTCGGCTCAA CCGTGAGAAA TAAGCTGGTG CGCCTCATGA CACACGTTGA CCTCGGAGTC 
AAGCAGATTG CTGCTGAGTT CCTCTTCGTC CTTTGCAAAG AGCGAGTGGA TAGTCTGCTG 
AAGTACACTG GCTATGGGAA CGCTGCAGGA CTGCTGGCTG CCAGGGGCCT CTTGGCTGGA 
GGAAGAGGAG ACAATTGGTA CTCAGAGGAC GAGGACACGG ACACTGAGGA ATACAAAAAC 
GCAAAACCAA ACATTAATCT TATCACTGGT CATTTAGAGG AACCAATGCC AAACCCTATA 
GATGAAATGA CAGAAGAACA AAAAGAATAT GAAGCCATGA AACTTGTCAA CATGCTTGAT 
AAACTCTCCA GAGAGGAGTT GCTTAAACCA ATGGGACTAA AACCTGACGG GACAATAACG 
CCTTTGGAGG AAGCGCTCAG CCAGTATTCT GTCATCGAAG AGACCAGCTC GGACACAGAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ric8b ( XM_017594824.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017450313.1 Rattus norvegicus RIC8 guanine nucleotide exchange factor B (Ric8b), transcript variant X5, mRNA.

Price & Availability↑

CloneID	ORa30022
Clone ID Related Accession (Same CDS sequence)	XM_017594824.1 , XM_008765234.2
Accession Version	XM_017594824.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1611bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	synembryn-B isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005106.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAAG AGCGCGCCCT CTACATCGTC CGGGCCGGCG AGGCGGGGGC TATCGAGCGG 
GTCCTGAGAG ATTACAGCGA CAAGCATAGG GCTACTTTCA AATTTGAATC AGCAGATGAA 
GATAAAAGAA AGAAACTCTG TGAAGGCATA TTTAAGGTCC TGGTAAAGGG TGTCCCAACA 
ACATGCCAAG TGCCCTGCCT GGAAGTTCTC CGCATTCTCT CCAGAGACAA AAAGATTTTA 
GCTCCTGTAA CAACTGAGGA AAATATGCAA ATACTGCTGA GACTGGCCAA GCTCCATGAG 
TCGGACGATT CTTTGGAGAA GGTGTCAGAG TTCCCGGTCA TTGTGGAGTC ATTGAAATGT 
CTTTGTAACA TCGTGTTCAA CAGTCAGGTG GCACAGCAGC TTAGCCTGGA ACTGAATCTC 
GCTGCAAAGC TCTGTAACCT CCTGAGGAAG TGCAAGGACC GAAAATTTAT CAATGACATA 
AAGTGCTTTG ACCTGCGCTT GCTCTTTGTC CTGTCACTTT TGCACACTGA CATCAGGTCA 
CAATTGCGCT ATGAGCTCCA GGGACTGCCG CTGCTAACCC AGATCTTGGA AAGTGCCTTT 
AGCATCAAGT GGACCGATGA GTATGAGTCG GCCATAGACC ATAGTGGACC TCCTCTCTCA 
CCTCAGGAGA CAGACTGTGC CATTGAGGCC CTCAAAGCTC TGTTCAATGT GACGGTAGAC 
AGTTGGAAGG TGCATAAAGA GAGCGATTCT CATCAGTTCC GTGTCATGGC CGCTGTCCTT 
CGCCACTGTT TACTAATTGT AGGTCCAACC GAAGACAAAA CGGAAGAGCT GCACAGTAAT 
GCCGTCAACC TTTTAAGCAA TGTTCCAGTC TCTTGTTTGG ATGTTCTCAT TTGTCCATTA 
ACCCATGAAG AAACAGCCCA GGAGGCAGCA ACTCTAGACG AACTGCCCGG TGATAAGACA 
GCAGAGAAAG ACACGGCTTT GAAGAGCAGT GCCATGGTAT ACAATGGCAT GAACATGGAG 
GCCATCCACG TTTTACTCAG TTTTATGGAG AAGAGGATTG ATAAGGGAAG CAGCTATAGA 
GAGGGTCTAA CTCCAGTTCT CAGCTTATTA ACAGAATGTT CCCGAGCCCA TCGGAACATC 
CGAAAATTTC TCAAAGATCA GGTGTTACCA CCTTTGAGGG ATGTGACAAA TCGACCAGAA 
GTCGGCTCAA CCGTGAGAAA TAAGCTGGTG CGCCTCATGA CACACGTTGA CCTCGGAGTC 
AAGCAGATTG CTGCTGAGTT CCTCTTCGTC CTTTGCAAAG AGCGAGTGGA TAGTCTGCTG 
AAGTACACTG GCTATGGGAA CGCTGCAGGA CTGCTGGCTG CCAGGGGCCT CTTGGCTGGA 
GGAAGAGGAG ACAATTGGTA CTCAGAGGAC GAGGACACGG ACACTGAGGA ATACAAAAAC 
GCAAAACCAA ACATTAATCT TATCACTGGT CATTTAGAGG AACCAATGCC AAACCCTATA 
GATGAAATGA CAGAAGAACA AAAAGAATAT GAAGCCATGA AACTTGTCAA CATGCTTGAT 
AAACTCTCCA GGAGAGCGGA TGTGAAGGAC CTGCACCAGG ATGGTGGTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017450313.1
CDS	688..2298
Translation	MDEERALYIVRAGEAGAIERVLRDYSDKHRATFKFESADEDKRKKLCEGIFKVLVKGVPTTCQVPCLEVLRILSRDKKILAPVTTEENMQILLRLAKLHESDDSLEKVSEFPVIVESLKCLCNIVFNSQVAQQLSLELNLAAKLCNLLRKCKDRKFINDIKCFDLRLLFVLSLLHTDIRSQLRYELQGLPLLTQILESAFSIKWTDEYESAIDHSGPPLSPQETDCAIEALKALFNVTVDSWKVHKESDSHQFRVMAAVLRHCLLIVGPTEDKTEELHSNAVNLLSNVPVSCLDVLICPLTHEETAQEAATLDELPGDKTAEKDTALKSSAMVYNGMNMEAIHVLLSFMEKRIDKGSSYREGLTPVLSLLTECSRAHRNIRKFLKDQVLPPLRDVTNRPEVGSTVRNKLVRLMTHVDLGVKQIAAEFLFVLCKERVDSLLKYTGYGNAAGLLAARGLLAGGRGDNWYSEDEDTDTEEYKNAKPNINLITGHLEEPMPNPIDEMTEEQKEYEAMKLVNMLDKLSRRADVKDLHQDGG

Target ORF information:

RefSeq Version	XM_017594824.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus RIC8 guanine nucleotide exchange factor B (Ric8b), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017594824.1

ORF Insert Sequence:

ATGGATGAAG AGCGCGCCCT CTACATCGTC CGGGCCGGCG AGGCGGGGGC TATCGAGCGG 
GTCCTGAGAG ATTACAGCGA CAAGCATAGG GCTACTTTCA AATTTGAATC AGCAGATGAA 
GATAAAAGAA AGAAACTCTG TGAAGGCATA TTTAAGGTCC TGGTAAAGGG TGTCCCAACA 
ACATGCCAAG TGCCCTGCCT GGAAGTTCTC CGCATTCTCT CCAGAGACAA AAAGATTTTA 
GCTCCTGTAA CAACTGAGGA AAATATGCAA ATACTGCTGA GACTGGCCAA GCTCCATGAG 
TCGGACGATT CTTTGGAGAA GGTGTCAGAG TTCCCGGTCA TTGTGGAGTC ATTGAAATGT 
CTTTGTAACA TCGTGTTCAA CAGTCAGGTG GCACAGCAGC TTAGCCTGGA ACTGAATCTC 
GCTGCAAAGC TCTGTAACCT CCTGAGGAAG TGCAAGGACC GAAAATTTAT CAATGACATA 
AAGTGCTTTG ACCTGCGCTT GCTCTTTGTC CTGTCACTTT TGCACACTGA CATCAGGTCA 
CAATTGCGCT ATGAGCTCCA GGGACTGCCG CTGCTAACCC AGATCTTGGA AAGTGCCTTT 
AGCATCAAGT GGACCGATGA GTATGAGTCG GCCATAGACC ATAGTGGACC TCCTCTCTCA 
CCTCAGGAGA CAGACTGTGC CATTGAGGCC CTCAAAGCTC TGTTCAATGT GACGGTAGAC 
AGTTGGAAGG TGCATAAAGA GAGCGATTCT CATCAGTTCC GTGTCATGGC CGCTGTCCTT 
CGCCACTGTT TACTAATTGT AGGTCCAACC GAAGACAAAA CGGAAGAGCT GCACAGTAAT 
GCCGTCAACC TTTTAAGCAA TGTTCCAGTC TCTTGTTTGG ATGTTCTCAT TTGTCCATTA 
ACCCATGAAG AAACAGCCCA GGAGGCAGCA ACTCTAGACG AACTGCCCGG TGATAAGACA 
GCAGAGAAAG ACACGGCTTT GAAGAGCAGT GCCATGGTAT ACAATGGCAT GAACATGGAG 
GCCATCCACG TTTTACTCAG TTTTATGGAG AAGAGGATTG ATAAGGGAAG CAGCTATAGA 
GAGGGTCTAA CTCCAGTTCT CAGCTTATTA ACAGAATGTT CCCGAGCCCA TCGGAACATC 
CGAAAATTTC TCAAAGATCA GGTGTTACCA CCTTTGAGGG ATGTGACAAA TCGACCAGAA 
GTCGGCTCAA CCGTGAGAAA TAAGCTGGTG CGCCTCATGA CACACGTTGA CCTCGGAGTC 
AAGCAGATTG CTGCTGAGTT CCTCTTCGTC CTTTGCAAAG AGCGAGTGGA TAGTCTGCTG 
AAGTACACTG GCTATGGGAA CGCTGCAGGA CTGCTGGCTG CCAGGGGCCT CTTGGCTGGA 
GGAAGAGGAG ACAATTGGTA CTCAGAGGAC GAGGACACGG ACACTGAGGA ATACAAAAAC 
GCAAAACCAA ACATTAATCT TATCACTGGT CATTTAGAGG AACCAATGCC AAACCCTATA 
GATGAAATGA CAGAAGAACA AAAAGAATAT GAAGCCATGA AACTTGTCAA CATGCTTGAT 
AAACTCTCCA GGAGAGCGGA TGTGAAGGAC CTGCACCAGG ATGGTGGTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ric8b ( XM_008765234.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008763456.1 Rattus norvegicus RIC8 guanine nucleotide exchange factor B (Ric8b), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ORa30022
Clone ID Related Accession (Same CDS sequence)	XM_017594824.1 , XM_008765234.2
Accession Version	XM_008765234.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1611bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	synembryn-B isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005106.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008765234.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAAG AGCGCGCCCT CTACATCGTC CGGGCCGGCG AGGCGGGGGC TATCGAGCGG 
GTCCTGAGAG ATTACAGCGA CAAGCATAGG GCTACTTTCA AATTTGAATC AGCAGATGAA 
GATAAAAGAA AGAAACTCTG TGAAGGCATA TTTAAGGTCC TGGTAAAGGG TGTCCCAACA 
ACATGCCAAG TGCCCTGCCT GGAAGTTCTC CGCATTCTCT CCAGAGACAA AAAGATTTTA 
GCTCCTGTAA CAACTGAGGA AAATATGCAA ATACTGCTGA GACTGGCCAA GCTCCATGAG 
TCGGACGATT CTTTGGAGAA GGTGTCAGAG TTCCCGGTCA TTGTGGAGTC ATTGAAATGT 
CTTTGTAACA TCGTGTTCAA CAGTCAGGTG GCACAGCAGC TTAGCCTGGA ACTGAATCTC 
GCTGCAAAGC TCTGTAACCT CCTGAGGAAG TGCAAGGACC GAAAATTTAT CAATGACATA 
AAGTGCTTTG ACCTGCGCTT GCTCTTTGTC CTGTCACTTT TGCACACTGA CATCAGGTCA 
CAATTGCGCT ATGAGCTCCA GGGACTGCCG CTGCTAACCC AGATCTTGGA AAGTGCCTTT 
AGCATCAAGT GGACCGATGA GTATGAGTCG GCCATAGACC ATAGTGGACC TCCTCTCTCA 
CCTCAGGAGA CAGACTGTGC CATTGAGGCC CTCAAAGCTC TGTTCAATGT GACGGTAGAC 
AGTTGGAAGG TGCATAAAGA GAGCGATTCT CATCAGTTCC GTGTCATGGC CGCTGTCCTT 
CGCCACTGTT TACTAATTGT AGGTCCAACC GAAGACAAAA CGGAAGAGCT GCACAGTAAT 
GCCGTCAACC TTTTAAGCAA TGTTCCAGTC TCTTGTTTGG ATGTTCTCAT TTGTCCATTA 
ACCCATGAAG AAACAGCCCA GGAGGCAGCA ACTCTAGACG AACTGCCCGG TGATAAGACA 
GCAGAGAAAG ACACGGCTTT GAAGAGCAGT GCCATGGTAT ACAATGGCAT GAACATGGAG 
GCCATCCACG TTTTACTCAG TTTTATGGAG AAGAGGATTG ATAAGGGAAG CAGCTATAGA 
GAGGGTCTAA CTCCAGTTCT CAGCTTATTA ACAGAATGTT CCCGAGCCCA TCGGAACATC 
CGAAAATTTC TCAAAGATCA GGTGTTACCA CCTTTGAGGG ATGTGACAAA TCGACCAGAA 
GTCGGCTCAA CCGTGAGAAA TAAGCTGGTG CGCCTCATGA CACACGTTGA CCTCGGAGTC 
AAGCAGATTG CTGCTGAGTT CCTCTTCGTC CTTTGCAAAG AGCGAGTGGA TAGTCTGCTG 
AAGTACACTG GCTATGGGAA CGCTGCAGGA CTGCTGGCTG CCAGGGGCCT CTTGGCTGGA 
GGAAGAGGAG ACAATTGGTA CTCAGAGGAC GAGGACACGG ACACTGAGGA ATACAAAAAC 
GCAAAACCAA ACATTAATCT TATCACTGGT CATTTAGAGG AACCAATGCC AAACCCTATA 
GATGAAATGA CAGAAGAACA AAAAGAATAT GAAGCCATGA AACTTGTCAA CATGCTTGAT 
AAACTCTCCA GGAGAGCGGA TGTGAAGGAC CTGCACCAGG ATGGTGGTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008763456.1
CDS	688..2298
Translation	MDEERALYIVRAGEAGAIERVLRDYSDKHRATFKFESADEDKRKKLCEGIFKVLVKGVPTTCQVPCLEVLRILSRDKKILAPVTTEENMQILLRLAKLHESDDSLEKVSEFPVIVESLKCLCNIVFNSQVAQQLSLELNLAAKLCNLLRKCKDRKFINDIKCFDLRLLFVLSLLHTDIRSQLRYELQGLPLLTQILESAFSIKWTDEYESAIDHSGPPLSPQETDCAIEALKALFNVTVDSWKVHKESDSHQFRVMAAVLRHCLLIVGPTEDKTEELHSNAVNLLSNVPVSCLDVLICPLTHEETAQEAATLDELPGDKTAEKDTALKSSAMVYNGMNMEAIHVLLSFMEKRIDKGSSYREGLTPVLSLLTECSRAHRNIRKFLKDQVLPPLRDVTNRPEVGSTVRNKLVRLMTHVDLGVKQIAAEFLFVLCKERVDSLLKYTGYGNAAGLLAARGLLAGGRGDNWYSEDEDTDTEEYKNAKPNINLITGHLEEPMPNPIDEMTEEQKEYEAMKLVNMLDKLSRRADVKDLHQDGG

Target ORF information:

RefSeq Version	XM_008765234.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus RIC8 guanine nucleotide exchange factor B (Ric8b), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008765234.2

ORF Insert Sequence:

ATGGATGAAG AGCGCGCCCT CTACATCGTC CGGGCCGGCG AGGCGGGGGC TATCGAGCGG 
GTCCTGAGAG ATTACAGCGA CAAGCATAGG GCTACTTTCA AATTTGAATC AGCAGATGAA 
GATAAAAGAA AGAAACTCTG TGAAGGCATA TTTAAGGTCC TGGTAAAGGG TGTCCCAACA 
ACATGCCAAG TGCCCTGCCT GGAAGTTCTC CGCATTCTCT CCAGAGACAA AAAGATTTTA 
GCTCCTGTAA CAACTGAGGA AAATATGCAA ATACTGCTGA GACTGGCCAA GCTCCATGAG 
TCGGACGATT CTTTGGAGAA GGTGTCAGAG TTCCCGGTCA TTGTGGAGTC ATTGAAATGT 
CTTTGTAACA TCGTGTTCAA CAGTCAGGTG GCACAGCAGC TTAGCCTGGA ACTGAATCTC 
GCTGCAAAGC TCTGTAACCT CCTGAGGAAG TGCAAGGACC GAAAATTTAT CAATGACATA 
AAGTGCTTTG ACCTGCGCTT GCTCTTTGTC CTGTCACTTT TGCACACTGA CATCAGGTCA 
CAATTGCGCT ATGAGCTCCA GGGACTGCCG CTGCTAACCC AGATCTTGGA AAGTGCCTTT 
AGCATCAAGT GGACCGATGA GTATGAGTCG GCCATAGACC ATAGTGGACC TCCTCTCTCA 
CCTCAGGAGA CAGACTGTGC CATTGAGGCC CTCAAAGCTC TGTTCAATGT GACGGTAGAC 
AGTTGGAAGG TGCATAAAGA GAGCGATTCT CATCAGTTCC GTGTCATGGC CGCTGTCCTT 
CGCCACTGTT TACTAATTGT AGGTCCAACC GAAGACAAAA CGGAAGAGCT GCACAGTAAT 
GCCGTCAACC TTTTAAGCAA TGTTCCAGTC TCTTGTTTGG ATGTTCTCAT TTGTCCATTA 
ACCCATGAAG AAACAGCCCA GGAGGCAGCA ACTCTAGACG AACTGCCCGG TGATAAGACA 
GCAGAGAAAG ACACGGCTTT GAAGAGCAGT GCCATGGTAT ACAATGGCAT GAACATGGAG 
GCCATCCACG TTTTACTCAG TTTTATGGAG AAGAGGATTG ATAAGGGAAG CAGCTATAGA 
GAGGGTCTAA CTCCAGTTCT CAGCTTATTA ACAGAATGTT CCCGAGCCCA TCGGAACATC 
CGAAAATTTC TCAAAGATCA GGTGTTACCA CCTTTGAGGG ATGTGACAAA TCGACCAGAA 
GTCGGCTCAA CCGTGAGAAA TAAGCTGGTG CGCCTCATGA CACACGTTGA CCTCGGAGTC 
AAGCAGATTG CTGCTGAGTT CCTCTTCGTC CTTTGCAAAG AGCGAGTGGA TAGTCTGCTG 
AAGTACACTG GCTATGGGAA CGCTGCAGGA CTGCTGGCTG CCAGGGGCCT CTTGGCTGGA 
GGAAGAGGAG ACAATTGGTA CTCAGAGGAC GAGGACACGG ACACTGAGGA ATACAAAAAC 
GCAAAACCAA ACATTAATCT TATCACTGGT CATTTAGAGG AACCAATGCC AAACCCTATA 
GATGAAATGA CAGAAGAACA AAAAGAATAT GAAGCCATGA AACTTGTCAA CATGCTTGAT 
AAACTCTCCA GGAGAGCGGA TGTGAAGGAC CTGCACCAGG ATGGTGGTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ric8b ( XM_006241182.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006241244.1 Rattus norvegicus RIC8 guanine nucleotide exchange factor B (Ric8b), transcript variant X6, mRNA.

Price & Availability↑

CloneID	ORa30023
Clone ID Related Accession (Same CDS sequence)	XM_006241182.3
Accession Version	XM_006241182.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1587bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	synembryn-B isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005106.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006241182.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAAG AGCGCGCCCT CTACATCGTC CGGGCCGGCG AGGCGGGGGC TATCGAGCGG 
GTCCTGAGAG ATTACAGCGA CAAGCATAGG GCTACTTTCA AATTTGAATC AGCAGATGAA 
GATAAAAGAA AGAAACTCTG TGAAGGCATA TTTAAGGTCC TGGTAAAGGG TGTCCCAACA 
ACATGCCAAG TGCCCTGCCT GGAAGTTCTC CGCATTCTCT CCAGAGACAA AAAGATTTTA 
GCTCCTGTAA CAACTGAGGA AAATATGCAA ATACTGCTGA GACTGGCCAA GCTCCATGAG 
TCGGACGATT CTTTGGAGAA GGTGTCAGAG TTCCCGGTCA TTGTGGAGTC ATTGAAATGT 
CTTTGTAACA TCGTGTTCAA CAGTCAGGTG GCACAGCAGC TTAGCCTGGA ACTGAATCTC 
GCTGCAAAGC TCTGTAACCT CCTGAGGAAG TGCAAGGACC GAAAATTTAT CAATGACATA 
AAGTGCTTTG ACCTGCGCTT GCTCTTTGTC CTGTCACTTT TGCACACTGA CATCAGGTCA 
CAATTGCGCT ATGAGCTCCA GGGACTGCCG CTGCTAACCC AGATCTTGGA AAGTGCCTTT 
AGCATCAAGT GGACCGATGA GTATGAGTCG GCCATAGACC ATAGTGGACC TCCTCTCTCA 
CCTCAGGAGA CAGACTGTGC CATTGAGGCC CTCAAAGCTC TGTTCAATGT GACGGTAGAC 
AGTTGGAAGG TGCATAAAGA GAGCGATTCT CATCAGTTCC GTGTCATGGC CGCTGTCCTT 
CGCCACTGTT TACTAATTGT AGGTCCAACC GAAGACAAAA CGGAAGAGCT GCACAGTAAT 
GCCGTCAACC TTTTAAGCAA TGTTCCAGTC TCTTGTTTGG ATGTTCTCAT TTGTCCATTA 
ACCCATGAAG AAACAGCCCA GGAGGCAGCA ACTCTAGACG AACTGCCCGG TGATAAGACA 
GCAGAGAAAG ACACGGCTTT GAAGAGCAGT GCCATGGTAT ACAATGGCAT GAACATGGAG 
GCCATCCACG TTTTACTCAG TTTTATGGAG AAGAGGATTG ATAAGGTGTT ACCACCTTTG 
AGGGATGTGA CAAATCGACC AGAAGTCGGC TCAACCGTGA GAAATAAGCT GGTGCGCCTC 
ATGACACACG TTGACCTCGG AGTCAAGCAG ATTGCTGCTG AGTTCCTCTT CGTCCTTTGC 
AAAGAGCGAG TGGATAGTCT GCTGAAGTAC ACTGGCTATG GGAACGCTGC AGGACTGCTG 
GCTGCCAGGG GCCTCTTGGC TGGAGGAAGA GGAGACAATT GGTACTCAGA GGACGAGGAC 
ACGGACACTG AGGAATACAA AAACGCAAAA CCAAACATTA ATCTTATCAC TGGTCATTTA 
GAGGAACCAA TGCCAAACCC TATAGATGAA ATGACAGAAG AACAAAAAGA ATATGAAGCC 
ATGAAACTTG TCAACATGCT TGATAAACTC TCCAGAGAGG AGTTGCTTAA ACCAATGGGA 
CTAAAACCTG ACGGGACAAT AACGCCTTTG GAGGAAGCGC TCAGCCAGTA TTCTGTCATC 
GAAGAGACCA GCTCGGACAC AGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006241244.1
CDS	689..2275
Translation	MDEERALYIVRAGEAGAIERVLRDYSDKHRATFKFESADEDKRKKLCEGIFKVLVKGVPTTCQVPCLEVLRILSRDKKILAPVTTEENMQILLRLAKLHESDDSLEKVSEFPVIVESLKCLCNIVFNSQVAQQLSLELNLAAKLCNLLRKCKDRKFINDIKCFDLRLLFVLSLLHTDIRSQLRYELQGLPLLTQILESAFSIKWTDEYESAIDHSGPPLSPQETDCAIEALKALFNVTVDSWKVHKESDSHQFRVMAAVLRHCLLIVGPTEDKTEELHSNAVNLLSNVPVSCLDVLICPLTHEETAQEAATLDELPGDKTAEKDTALKSSAMVYNGMNMEAIHVLLSFMEKRIDKVLPPLRDVTNRPEVGSTVRNKLVRLMTHVDLGVKQIAAEFLFVLCKERVDSLLKYTGYGNAAGLLAARGLLAGGRGDNWYSEDEDTDTEEYKNAKPNINLITGHLEEPMPNPIDEMTEEQKEYEAMKLVNMLDKLSREELLKPMGLKPDGTITPLEEALSQYSVIEETSSDTD

Target ORF information:

RefSeq Version	XM_006241182.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus RIC8 guanine nucleotide exchange factor B (Ric8b), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006241182.3

ORF Insert Sequence:

ATGGATGAAG AGCGCGCCCT CTACATCGTC CGGGCCGGCG AGGCGGGGGC TATCGAGCGG 
GTCCTGAGAG ATTACAGCGA CAAGCATAGG GCTACTTTCA AATTTGAATC AGCAGATGAA 
GATAAAAGAA AGAAACTCTG TGAAGGCATA TTTAAGGTCC TGGTAAAGGG TGTCCCAACA 
ACATGCCAAG TGCCCTGCCT GGAAGTTCTC CGCATTCTCT CCAGAGACAA AAAGATTTTA 
GCTCCTGTAA CAACTGAGGA AAATATGCAA ATACTGCTGA GACTGGCCAA GCTCCATGAG 
TCGGACGATT CTTTGGAGAA GGTGTCAGAG TTCCCGGTCA TTGTGGAGTC ATTGAAATGT 
CTTTGTAACA TCGTGTTCAA CAGTCAGGTG GCACAGCAGC TTAGCCTGGA ACTGAATCTC 
GCTGCAAAGC TCTGTAACCT CCTGAGGAAG TGCAAGGACC GAAAATTTAT CAATGACATA 
AAGTGCTTTG ACCTGCGCTT GCTCTTTGTC CTGTCACTTT TGCACACTGA CATCAGGTCA 
CAATTGCGCT ATGAGCTCCA GGGACTGCCG CTGCTAACCC AGATCTTGGA AAGTGCCTTT 
AGCATCAAGT GGACCGATGA GTATGAGTCG GCCATAGACC ATAGTGGACC TCCTCTCTCA 
CCTCAGGAGA CAGACTGTGC CATTGAGGCC CTCAAAGCTC TGTTCAATGT GACGGTAGAC 
AGTTGGAAGG TGCATAAAGA GAGCGATTCT CATCAGTTCC GTGTCATGGC CGCTGTCCTT 
CGCCACTGTT TACTAATTGT AGGTCCAACC GAAGACAAAA CGGAAGAGCT GCACAGTAAT 
GCCGTCAACC TTTTAAGCAA TGTTCCAGTC TCTTGTTTGG ATGTTCTCAT TTGTCCATTA 
ACCCATGAAG AAACAGCCCA GGAGGCAGCA ACTCTAGACG AACTGCCCGG TGATAAGACA 
GCAGAGAAAG ACACGGCTTT GAAGAGCAGT GCCATGGTAT ACAATGGCAT GAACATGGAG 
GCCATCCACG TTTTACTCAG TTTTATGGAG AAGAGGATTG ATAAGGTGTT ACCACCTTTG 
AGGGATGTGA CAAATCGACC AGAAGTCGGC TCAACCGTGA GAAATAAGCT GGTGCGCCTC 
ATGACACACG TTGACCTCGG AGTCAAGCAG ATTGCTGCTG AGTTCCTCTT CGTCCTTTGC 
AAAGAGCGAG TGGATAGTCT GCTGAAGTAC ACTGGCTATG GGAACGCTGC AGGACTGCTG 
GCTGCCAGGG GCCTCTTGGC TGGAGGAAGA GGAGACAATT GGTACTCAGA GGACGAGGAC 
ACGGACACTG AGGAATACAA AAACGCAAAA CCAAACATTA ATCTTATCAC TGGTCATTTA 
GAGGAACCAA TGCCAAACCC TATAGATGAA ATGACAGAAG AACAAAAAGA ATATGAAGCC 
ATGAAACTTG TCAACATGCT TGATAAACTC TCCAGAGAGG AGTTGCTTAA ACCAATGGGA 
CTAAAACCTG ACGGGACAAT AACGCCTTTG GAGGAAGCGC TCAGCCAGTA TTCTGTCATC 
GAAGAGACCA GCTCGGACAC AGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ric8b ( XM_008765236.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008763458.1 Rattus norvegicus RIC8 guanine nucleotide exchange factor B (Ric8b), transcript variant X7, mRNA.

Price & Availability↑

CloneID	ORa47351
Clone ID Related Accession (Same CDS sequence)	XM_008765236.2
Accession Version	XM_008765236.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1584bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	synembryn-B isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005106.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008765236.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAAG AGCGCGCCCT CTACATCGTC CGGGCCGGCG AGGCGGGGGC TATCGAGCGG 
GTCCTGAGAG ATTACAGCGA CAAGCATAGG GCTACTTTCA AATTTGAATC AGCAGATGAA 
GATAAAAGAA AGAAACTCTG TGAAGGCATA TTTAAGGTCC TGGTAAAGGG TGTCCCAACA 
ACATGCCAAG TGCCCTGCCT GGAAGTTCTC CGCATTCTCT CCAGAGACAA AAAGATTTTA 
GCTCCTGTAA CAACTGAGGA AAATATGCAA ATACTGCTGA GACTGGCCAA GCTCCATGAG 
TCGGACGATT CTTTGGAGAA GGTGTCAGAG TTCCCGGTCA TTGTGGAGTC ATTGAAATGT 
CTTTGTAACA TCGTGTTCAA CAGTCAGGTG GCACAGCAGC TTAGCCTGGA ACTGAATCTC 
GCTGCAAAGC TCTGTAACCT CCTGAGGAAG TGCAAGGACC GAAAATTTAT CAATGACATA 
AAGTGCTTTG ACCTGCGCTT GCTCTTTGTC CTGTCACTTT TGCACACTGA CATCAGGTCA 
CAATTGCGCT ATGAGCTCCA GGGACTGCCG CTGCTAACCC AGATCTTGGA AAGTGCCTTT 
AGCATCAAGT GGACCGATGA GTATGAGTCG GCCATAGACC ATAGTGGACC TCCTCTCTCA 
CCTCAGGAGA CAGACTGTGC CATTGAGGCC CTCAAAGCTC TGTTCAATGT GACGGTAGAC 
AGTTGGAAGG TGCATAAAGA GAGCGATTCT CATCAGTTCC GTGTCATGGC CGCTGTCCTT 
CGCCACTGTT TACTAATTGT AGGTCCAACC GAAGACAAAA CGGAAGAGCT GCACAGTAAT 
GCCGTCAACC TTTTAAGCAA TGTTCCAGTC TCTTGTTTGG ATGTTCTCAT TTGTCCATTA 
ACCCATGAAG AAACAGCCCA GGAGGCAGCA ACTCTAGACG AACTGCCCGG TGATAAGACA 
GCAGAGAAAG ACACGGCTTT GAAGAGCAGT GCCATGGTAT ACAATGGCAT GAACATGGAG 
GCCATCCACG TTTTACTCAG TTTTATGGAG AAGAGGATTG ATAAGGGAAG CAGCTATAGA 
GAGGGTCTAA CTCCAGTTCT CAGCTTATTA ACAGAATGTT CCCGAGCCCA TCGGAACATC 
CGAAAATTTC TCAAAGATCA GGTGTTACCA CCTTTGAGGG ATGTGACAAA TCGACCAGAA 
GTCGGCTCAA CCGTGAGAAA TAAGCTGGTG CGCCTCATGA CACACGTTGA CCTCGGAGTC 
AAGCAGATTG CTGCTGAGTT CCTCTTCGTC CTTTGCAAAG AGCGAGTGGA TAGTCTGCTG 
AAGTACACTG GCTATGGGAA CGCTGCAGGA CTGCTGGCTG CCAGGGGCCT CTTGGCTGGA 
GGAAGAGGAG ACAATTGGTA CTCAGAGGAC GAGGACACGG ACACTGAGGA ATACAAAAAC 
GCAAAACCAA ACATTAATCT TATCACTGGT CATTTAGAGG AACCAATGCC AAACCCTATA 
GATGAAATGA CAGAAGAACA AAAAGAATAT GAAGCCATGA AACTTGTCAA CATGCTTGAT 
AAACTCTCCA GACCCTGTCA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008763458.1
CDS	687..2270
Translation	MDEERALYIVRAGEAGAIERVLRDYSDKHRATFKFESADEDKRKKLCEGIFKVLVKGVPTTCQVPCLEVLRILSRDKKILAPVTTEENMQILLRLAKLHESDDSLEKVSEFPVIVESLKCLCNIVFNSQVAQQLSLELNLAAKLCNLLRKCKDRKFINDIKCFDLRLLFVLSLLHTDIRSQLRYELQGLPLLTQILESAFSIKWTDEYESAIDHSGPPLSPQETDCAIEALKALFNVTVDSWKVHKESDSHQFRVMAAVLRHCLLIVGPTEDKTEELHSNAVNLLSNVPVSCLDVLICPLTHEETAQEAATLDELPGDKTAEKDTALKSSAMVYNGMNMEAIHVLLSFMEKRIDKGSSYREGLTPVLSLLTECSRAHRNIRKFLKDQVLPPLRDVTNRPEVGSTVRNKLVRLMTHVDLGVKQIAAEFLFVLCKERVDSLLKYTGYGNAAGLLAARGLLAGGRGDNWYSEDEDTDTEEYKNAKPNINLITGHLEEPMPNPIDEMTEEQKEYEAMKLVNMLDKLSRPCH

Target ORF information:

RefSeq Version	XM_008765236.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus RIC8 guanine nucleotide exchange factor B (Ric8b), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008765236.2

ORF Insert Sequence:

ATGGATGAAG AGCGCGCCCT CTACATCGTC CGGGCCGGCG AGGCGGGGGC TATCGAGCGG 
GTCCTGAGAG ATTACAGCGA CAAGCATAGG GCTACTTTCA AATTTGAATC AGCAGATGAA 
GATAAAAGAA AGAAACTCTG TGAAGGCATA TTTAAGGTCC TGGTAAAGGG TGTCCCAACA 
ACATGCCAAG TGCCCTGCCT GGAAGTTCTC CGCATTCTCT CCAGAGACAA AAAGATTTTA 
GCTCCTGTAA CAACTGAGGA AAATATGCAA ATACTGCTGA GACTGGCCAA GCTCCATGAG 
TCGGACGATT CTTTGGAGAA GGTGTCAGAG TTCCCGGTCA TTGTGGAGTC ATTGAAATGT 
CTTTGTAACA TCGTGTTCAA CAGTCAGGTG GCACAGCAGC TTAGCCTGGA ACTGAATCTC 
GCTGCAAAGC TCTGTAACCT CCTGAGGAAG TGCAAGGACC GAAAATTTAT CAATGACATA 
AAGTGCTTTG ACCTGCGCTT GCTCTTTGTC CTGTCACTTT TGCACACTGA CATCAGGTCA 
CAATTGCGCT ATGAGCTCCA GGGACTGCCG CTGCTAACCC AGATCTTGGA AAGTGCCTTT 
AGCATCAAGT GGACCGATGA GTATGAGTCG GCCATAGACC ATAGTGGACC TCCTCTCTCA 
CCTCAGGAGA CAGACTGTGC CATTGAGGCC CTCAAAGCTC TGTTCAATGT GACGGTAGAC 
AGTTGGAAGG TGCATAAAGA GAGCGATTCT CATCAGTTCC GTGTCATGGC CGCTGTCCTT 
CGCCACTGTT TACTAATTGT AGGTCCAACC GAAGACAAAA CGGAAGAGCT GCACAGTAAT 
GCCGTCAACC TTTTAAGCAA TGTTCCAGTC TCTTGTTTGG ATGTTCTCAT TTGTCCATTA 
ACCCATGAAG AAACAGCCCA GGAGGCAGCA ACTCTAGACG AACTGCCCGG TGATAAGACA 
GCAGAGAAAG ACACGGCTTT GAAGAGCAGT GCCATGGTAT ACAATGGCAT GAACATGGAG 
GCCATCCACG TTTTACTCAG TTTTATGGAG AAGAGGATTG ATAAGGGAAG CAGCTATAGA 
GAGGGTCTAA CTCCAGTTCT CAGCTTATTA ACAGAATGTT CCCGAGCCCA TCGGAACATC 
CGAAAATTTC TCAAAGATCA GGTGTTACCA CCTTTGAGGG ATGTGACAAA TCGACCAGAA 
GTCGGCTCAA CCGTGAGAAA TAAGCTGGTG CGCCTCATGA CACACGTTGA CCTCGGAGTC 
AAGCAGATTG CTGCTGAGTT CCTCTTCGTC CTTTGCAAAG AGCGAGTGGA TAGTCTGCTG 
AAGTACACTG GCTATGGGAA CGCTGCAGGA CTGCTGGCTG CCAGGGGCCT CTTGGCTGGA 
GGAAGAGGAG ACAATTGGTA CTCAGAGGAC GAGGACACGG ACACTGAGGA ATACAAAAAC 
GCAAAACCAA ACATTAATCT TATCACTGGT CATTTAGAGG AACCAATGCC AAACCCTATA 
GATGAAATGA CAGAAGAACA AAAAGAATAT GAAGCCATGA AACTTGTCAA CATGCTTGAT 
AAACTCTCCA GACCCTGTCA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ric8b ( XM_008765237.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008763459.1 Rattus norvegicus RIC8 guanine nucleotide exchange factor B (Ric8b), transcript variant X8, mRNA.

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CloneID	ORa47352
Clone ID Related Accession (Same CDS sequence)	XM_008765237.2
Accession Version	XM_008765237.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1464bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	synembryn-B isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005106.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008765237.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAAG AGCGCGCCCT CTACATCGTC CGGGCCGGCG AGGCGGGGGC TATCGAGCGG 
GTCCTGAGAG ATTACAGCGA CAAGCATAGG GCTACTTTCA AATTTGAATC AGCAGATGAA 
GATAAAAGAA AGAAACTCTG TGAAGGCATA TTTAAGGTCC TGGTAAAGGG TGTCCCAACA 
ACATGCCAAG TGCCCTGCCT GGAAGTTCTC CGCATTCTCT CCAGAGACAA AAAGATTTTA 
GCTCCTGTAA CAACTGAGGA AAATATGCAA ATACTGCTGA GACTGGCCAA GCTCCATGAG 
TCGGACGATT CTTTGGAGAA GGTGTCAGAG TTCCCGGTCA TTGTGGAGTC ATTGAAATGT 
CTTTGTAACA TCGTGTTCAA CAGTCAGGTG GCACAGCAGC TTAGCCTGGA ACTGAATCTC 
GCTGCAAAGC TCTGTAACCT CCTGAGGAAG TGCAAGGACC GAAAATTTAT CAATGACATA 
AAGTGCTTTG ACCTGCGCTT GCTCTTTGTC CTGTCACTTT TGCACACTGA CATCAGGTCA 
CAATTGCGCT ATGAGCTCCA GGGACTGCCG CTGCTAACCC AGATCTTGGA AAGTGCCTTT 
AGCATCAAGT GGACCGATGA GTATGAGTCG GCCATAGACC ATAGTGGACC TCCTCTCTCA 
CCTCAGGAGA CAGACTGTGC CATTGAGGCC CTCAAAGCTC TGTTCAATGT GACGGTAGAC 
AGTTGGAAGG TGCATAAAGA GAGCGATTCT CATCAGTTCC GTGTCATGGC CGCTGTCCTT 
CGCCACTGTT TACTAATTGT AGGTCCAACC GAAGACAAAA CGGAAGAGCT GCACAGTAAT 
GCCGTCAACC TTTTAAGCAA TGTTCCAGTC TCTTGTTTGG ATGTTCTCAT TTGTCCATTA 
ACCCATGAAG AAACAGCCCA GGAGGCAGCA ACTCTAGACG AACTGCCCGG TGATAAGACA 
GCAGAGAAAG ACACGGCTTT GAAGAGCAGT GCCATGGTAT ACAATGGCAT GAACATGGAG 
GCCATCCACG TTTTACTCAG TTTTATGGAG AAGAGGATTG ATAAGGGAAG CAGCTATAGA 
GAGGGTCTAA CTCCAGTTCT CAGCTTATTA ACAGAATGTT CCCGAGCCCA TCGGAACATC 
CGAAAATTTC TCAAAGATCA GGTGTTACCA CCTTTGAGGG ATGTGACAAA TCGACCAGAA 
GTCGGCTCAA CCGTGAGAAA TAAGCTGGTG CGCCTCATGA CACACGTTGA CCTCGGAGTC 
AAGCAGATTG CTGCTGAGTT CCTCTTCGTC CTTTGCAAAG AGCGAGTGGA TAGTCTGCTG 
AAGTACACTG GCTATGGGAA CGCTGCAGGA CTGCTGGCTG CCAGGGGCCT CTTGGCTGGA 
GGAAGAGGAG ACAATTGGTA CTCAGAGGAC GAGGACACGG ACACTGAGGA ATACAAAAAC 
GCAAAACCAA AACCCTGTCA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008763459.1
CDS	687..2150
Translation	MDEERALYIVRAGEAGAIERVLRDYSDKHRATFKFESADEDKRKKLCEGIFKVLVKGVPTTCQVPCLEVLRILSRDKKILAPVTTEENMQILLRLAKLHESDDSLEKVSEFPVIVESLKCLCNIVFNSQVAQQLSLELNLAAKLCNLLRKCKDRKFINDIKCFDLRLLFVLSLLHTDIRSQLRYELQGLPLLTQILESAFSIKWTDEYESAIDHSGPPLSPQETDCAIEALKALFNVTVDSWKVHKESDSHQFRVMAAVLRHCLLIVGPTEDKTEELHSNAVNLLSNVPVSCLDVLICPLTHEETAQEAATLDELPGDKTAEKDTALKSSAMVYNGMNMEAIHVLLSFMEKRIDKGSSYREGLTPVLSLLTECSRAHRNIRKFLKDQVLPPLRDVTNRPEVGSTVRNKLVRLMTHVDLGVKQIAAEFLFVLCKERVDSLLKYTGYGNAAGLLAARGLLAGGRGDNWYSEDEDTDTEEYKNAKPKPCH

Target ORF information:

RefSeq Version	XM_008765237.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus RIC8 guanine nucleotide exchange factor B (Ric8b), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008765237.2

ORF Insert Sequence:

ATGGATGAAG AGCGCGCCCT CTACATCGTC CGGGCCGGCG AGGCGGGGGC TATCGAGCGG 
GTCCTGAGAG ATTACAGCGA CAAGCATAGG GCTACTTTCA AATTTGAATC AGCAGATGAA 
GATAAAAGAA AGAAACTCTG TGAAGGCATA TTTAAGGTCC TGGTAAAGGG TGTCCCAACA 
ACATGCCAAG TGCCCTGCCT GGAAGTTCTC CGCATTCTCT CCAGAGACAA AAAGATTTTA 
GCTCCTGTAA CAACTGAGGA AAATATGCAA ATACTGCTGA GACTGGCCAA GCTCCATGAG 
TCGGACGATT CTTTGGAGAA GGTGTCAGAG TTCCCGGTCA TTGTGGAGTC ATTGAAATGT 
CTTTGTAACA TCGTGTTCAA CAGTCAGGTG GCACAGCAGC TTAGCCTGGA ACTGAATCTC 
GCTGCAAAGC TCTGTAACCT CCTGAGGAAG TGCAAGGACC GAAAATTTAT CAATGACATA 
AAGTGCTTTG ACCTGCGCTT GCTCTTTGTC CTGTCACTTT TGCACACTGA CATCAGGTCA 
CAATTGCGCT ATGAGCTCCA GGGACTGCCG CTGCTAACCC AGATCTTGGA AAGTGCCTTT 
AGCATCAAGT GGACCGATGA GTATGAGTCG GCCATAGACC ATAGTGGACC TCCTCTCTCA 
CCTCAGGAGA CAGACTGTGC CATTGAGGCC CTCAAAGCTC TGTTCAATGT GACGGTAGAC 
AGTTGGAAGG TGCATAAAGA GAGCGATTCT CATCAGTTCC GTGTCATGGC CGCTGTCCTT 
CGCCACTGTT TACTAATTGT AGGTCCAACC GAAGACAAAA CGGAAGAGCT GCACAGTAAT 
GCCGTCAACC TTTTAAGCAA TGTTCCAGTC TCTTGTTTGG ATGTTCTCAT TTGTCCATTA 
ACCCATGAAG AAACAGCCCA GGAGGCAGCA ACTCTAGACG AACTGCCCGG TGATAAGACA 
GCAGAGAAAG ACACGGCTTT GAAGAGCAGT GCCATGGTAT ACAATGGCAT GAACATGGAG 
GCCATCCACG TTTTACTCAG TTTTATGGAG AAGAGGATTG ATAAGGGAAG CAGCTATAGA 
GAGGGTCTAA CTCCAGTTCT CAGCTTATTA ACAGAATGTT CCCGAGCCCA TCGGAACATC 
CGAAAATTTC TCAAAGATCA GGTGTTACCA CCTTTGAGGG ATGTGACAAA TCGACCAGAA 
GTCGGCTCAA CCGTGAGAAA TAAGCTGGTG CGCCTCATGA CACACGTTGA CCTCGGAGTC 
AAGCAGATTG CTGCTGAGTT CCTCTTCGTC CTTTGCAAAG AGCGAGTGGA TAGTCTGCTG 
AAGTACACTG GCTATGGGAA CGCTGCAGGA CTGCTGGCTG CCAGGGGCCT CTTGGCTGGA 
GGAAGAGGAG ACAATTGGTA CTCAGAGGAC GAGGACACGG ACACTGAGGA ATACAAAAAC 
GCAAAACCAA AACCCTGTCA TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

ARMS/Kidins220 and synembryn-B levels regulate NGF-mediated secretion.
Journal of cell science129(9)1866-77(2016 05)
L?pez-Benito S,Lillo C,Hern?ndez-Hern?ndez ?,Chao MV,Ar?valo JC

A functional N-terminal domain in C/EBP?-LAP* is required for interacting with SWI/SNF and to repress Ric-8B gene transcription in osteoblasts.
Journal of cellular physiology229(10)1521-8(2014 Oct)
Aguilar R,Grandy R,Meza D,Sepulveda H,Pihan P,van Wijnen AJ,Lian JB,Stein GS,Stein JL,Montecino M

Ric-8B is a GTP-dependent G protein alphas guanine nucleotide exchange factor.
The Journal of biological chemistry286(22)19932-42(2011 Jun)
Chan P,Gabay M,Wright FA,Tall GG

Human brain synembryn interacts with Gsalpha and Gqalpha and is translocated to the plasma membrane in response to isoproterenol and carbachol.
Journal of cellular physiology195(2)151-7(2003 May)
Klattenhoff C,Montecino M,Soto X,Guzm?n L,Romo X,Garc?a MA,Mellstrom B,Naranjo JR,Hinrichs MV,Olate J

Mammalian Ric-8A (synembryn) is a heterotrimeric Galpha protein guanine nucleotide exchange factor.
The Journal of biological chemistry278(10)8356-62(2003 Mar)
Tall GG,Krumins AM,Gilman AG

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Ric8b cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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