Dohh cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Dohh
Entrez Gene ID	314644
Full Name	deoxyhypusine hydroxylase
Synonyms	RGD1304783
General protein information	Preferred Names deoxyhypusine hydroxylase Names deoxyhypusine hydroxylase deoxyhypusine dioxygenase deoxyhypusine hydroxylase/monooxygenase deoxyhypusine monooxygenase
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 7 Map Location: 7q11

mRNA and Protein(s)

mRNA	Protein	Name
XM_006240938.2	XP_006241000.1	deoxyhypusine hydroxylase isoform X1
NM_001025006.1	NP_001020177.1	deoxyhypusine hydroxylase
XM_006240939.2	XP_006241001.1	deoxyhypusine hydroxylase isoform X1
XM_006240937.2	XP_006240999.1	deoxyhypusine hydroxylase isoform X1

Pathways from BioSystems

Homologies

Bos taurus (cattle)	DOHH	NP_001069354.1
Drosophila melanogaster (fruit fly)	nero	NP_651887.1
Magnaporthe oryzae (rice blast fungus)	MGG_01725	XP_003714722.1
Oryza sativa (rice)	Os12g0163300	NP_001066229.1
Homo sapiens (human)	DOHH	NP_112594.1
Pan troglodytes (chimpanzee)	DOHH	XP_512268.3
Mus musculus (house mouse)	Dohh	NP_598725.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002129	XP_320906.3
Caenorhabditis elegans (roundworm)	dohh-1	NP_496279.1
Kluyveromyces lactis	KLLA0B14080g	XP_452158.1
Arabidopsis thaliana (thale cress)	AT3G58180	NP_567062.1
	DOHH	XP_001100385.1
Canis lupus familiaris (dog)	LOC485060	XP_542178.2
Rattus norvegicus (Norway rat)	Dohh	NP_001020177.1
Saccharomyces cerevisiae (baker's yeast)	LIA1	NP_012604.1
Oryza sativa (rice)	Os12g0626100	NP_001067322.2
Gallus gallus (chicken)	DOHH	NP_001026584.1
Danio rerio (zebrafish)	dohh	NP_955857.1
Eremothecium gossypii	AGOS_AFR426C	NP_985973.2
Schizosaccharomyces pombe (fission yeast)	mmd1	NP_594654.1
Neurospora crassa	NCU05252	XP_961789.1
Xenopus tropicalis (tropical clawed frog)	LOC100145112	NP_001120098.1

Gene Ontology
Bibliography

Related articles in PubMed

Crystal Structure of the Peroxo-diiron(III) Intermediate of Deoxyhypusine Hydroxylase, an Oxygenase Involved in Hypusination.
Han Z, Sakai N, B?ttger LH, Klinke S, Hauber J, Trautwein AX, Hilgenfeld R
Structure (London, England : 1993)23(5)882-892(2015 May)

A novel mouse model for inhibition of DOHH-mediated hypusine modification reveals a crucial function in embryonic development, proliferation and oncogenic transformation.
Sievert H, P?llmann N, Miller KK, Hermans-Borgmeyer I, Venz S, Sendoel A, Preukschas M, Schweizer M, Boettcher S, Janiesch PC, Streichert T, Walther R, Hengartner MO, Manz MG, Br?mmendorf TH, Bokemeyer C, Braig M, Hauber J, Duncan KE, Balabanov S
Disease models & mechanisms7(8)963-76(2014 Aug)

Human deoxyhypusine hydroxylase, an enzyme involved in regulating cell growth, activates O2 with a nonheme diiron center.
Vu VV, Emerson JP, Martinho M, Kim YS, M?nck E, Park MH, Que L Jr
Proceedings of the National Academy of Sciences of the United States of America106(35)14814-9(2009 Sep)

Specificity of the deoxyhypusine hydroxylase-eukaryotic translation initiation factor (eIF5A) interaction: identification of amino acid residues of the enzyme required for binding of its substrate, deoxyhypusine-containing eIF5A.
Kang KR, Kim YS, Wolff EC, Park MH
The Journal of biological chemistry282(11)8300-8(2007 Mar)

Deoxyhypusine hydroxylase is a Fe(II)-dependent, HEAT-repeat enzyme. Identification of amino acid residues critical for Fe(II) binding and catalysis [corrected].
Kim YS, Kang KR, Wolff EC, Bell JK, McPhie P, Park MH
The Journal of biological chemistry281(19)13217-25(2006 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Dohh gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dohh cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa07000	NM_001025006.1 Latest version!	Rattus norvegicus deoxyhypusine hydroxylase (Dohh), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00
ORa07000	XM_006240938.2 Latest version!	Rattus norvegicus deoxyhypusine hydroxylase/monooxygenase (Dohh), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00
ORa07000	XM_006240937.2 Latest version!	Rattus norvegicus deoxyhypusine hydroxylase/monooxygenase (Dohh), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00
ORa07000	XM_006240939.2 Latest version!	Rattus norvegicus deoxyhypusine hydroxylase/monooxygenase (Dohh), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Dohh ( NM_001025006.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001020177.1 Rattus norvegicus deoxyhypusine hydroxylase (Dohh), mRNA.

Price & Availability↑

CloneID	ORa07000
Clone ID Related Accession (Same CDS sequence)	XM_006240938.2 , NM_001025006.1 , XM_006240939.2 , XM_006240937.2
Accession Version	NM_001025006.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 909bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-18
Organism	Rattus norvegicus(Norway rat)
Product	deoxyhypusine hydroxylase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC087658.1. On Jun 25, 2005 this sequence version replaced XM_234926.3. ##Evidence-Data-START## Transcript exon combination :: BC087658.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01906348, SAMN01906350 [ECO:0000348] ##Evidence-Data-END##

ATGGTAACAG AACAGGAGGT AGAGGCCATA GGGAAAACCC TAGTGGACTC CACGCAGCCC 
CTGCAGGCCC GCTTCCGTGC CCTGTTTACC CTGCGGGGAC TTGGTGGCCC CGATGCTATC 
TCCTGGATCA GCCGAGGCTT TGAGGACAGC TCTGCCCTTC TGAAGCACGA GCTAGCCTAC 
TGCCTGGGCC AGATGAGGGA CCCACGAGCC ATCCCTGTGC TGGTGTCCGT CCTACAGGAC 
AGGAACCAGG AGCCCATGGT GCGTCATGAG GCAGGGGAAG CACTGGGGGC GATTGGTAAC 
CCGAAAGTTC TAGGCCTCCT GAAGCAGTAT TCCACCGACC CAGTGGTTGA GGTGGCTGAG 
ACGTGCCAGC TCGCCGTTAG GCGTCTAGAG TGGCTGCAGC AGCACCCGGG GGAGGCCACA 
TGTGCAGGAC CCTACCTCTC AGTAGATCCT GCACCCCCTG CGGCAGAAGG TGATGTGGGG 
CGGCTGCGGG AGACCCTGCT GGATGAGGCG CAGCCACTCT TCGAGCGGTA CCGTGCCATG 
TTTGCCCTGC GCAATGTGGG TGGCAAGGAG GCTGCCTTGG CCCTGGCTGA AGGCCTGAAG 
TGTGGCAGTG CGCTCTTCCG ACACGAGGTG GGCTATGTGC TGGGTCAGCT GCAGCACGAA 
GCAGCCGTGT CTGAACTGGC AGCCACATTG GCACGGACCA CTGAGAGCCC CATGGTACGA 
CATGAGTGCG CAGAGGCCCT GGGTGCCATC GCCAGGCCTG CCTGCCTGGC TGCACTGCGG 
GAGTACATCA CGGACCCTGA GCGAGTGGTG CGGGAGAGCT GCGAGGTAGC ACTGGACATG 
TATGAGTATG AAAATGGCCA GGACTTCCAG TACGCAGATG GGCTCGAGCG CCTGAGGCCA 
CCGCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001020177.1
CDS	117..1025
Translation	MVTEQEVEAIGKTLVDSTQPLQARFRALFTLRGLGGPDAISWISRGFEDSSALLKHELAYCLGQMRDPRAIPVLVSVLQDRNQEPMVRHEAGEALGAIGNPKVLGLLKQYSTDPVVEVAETCQLAVRRLEWLQQHPGEATCAGPYLSVDPAPPAAEGDVGRLRETLLDEAQPLFERYRAMFALRNVGGKEAALALAEGLKCGSALFRHEVGYVLGQLQHEAAVSELAATLARTTESPMVRHECAEALGAIARPACLAALREYITDPERVVRESCEVALDMYEYENGQDFQYADGLERLRPPP

Target ORF information:

RefSeq Version	NM_001025006.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus deoxyhypusine hydroxylase (Dohh), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001025006.1

ORF Insert Sequence:

ATGGTAACAG AACAGGAGGT AGAGGCCATA GGGAAAACCC TAGTGGACTC CACGCAGCCC 
CTGCAGGCCC GCTTCCGTGC CCTGTTTACC CTGCGGGGAC TTGGTGGCCC CGATGCTATC 
TCCTGGATCA GCCGAGGCTT TGAGGACAGC TCTGCCCTTC TGAAGCACGA GCTAGCCTAC 
TGCCTGGGCC AGATGAGGGA CCCACGAGCC ATCCCTGTGC TGGTGTCCGT CCTACAGGAC 
AGGAACCAGG AGCCCATGGT GCGTCATGAG GCAGGGGAAG CACTGGGGGC GATTGGTAAC 
CCGAAAGTTC TAGGCCTCCT GAAGCAGTAT TCCACCGACC CAGTGGTTGA GGTGGCTGAG 
ACGTGCCAGC TCGCCGTTAG GCGTCTAGAG TGGCTGCAGC AGCACCCGGG GGAGGCCACA 
TGTGCAGGAC CCTACCTCTC AGTAGATCCT GCACCCCCTG CGGCAGAAGG TGATGTGGGG 
CGGCTGCGGG AGACCCTGCT GGATGAGGCG CAGCCACTCT TCGAGCGGTA CCGTGCCATG 
TTTGCCCTGC GCAATGTGGG TGGCAAGGAG GCTGCCTTGG CCCTGGCTGA AGGCCTGAAG 
TGTGGCAGTG CGCTCTTCCG ACACGAGGTG GGCTATGTGC TGGGTCAGCT GCAGCACGAA 
GCAGCCGTGT CTGAACTGGC AGCCACATTG GCACGGACCA CTGAGAGCCC CATGGTACGA 
CATGAGTGCG CAGAGGCCCT GGGTGCCATC GCCAGGCCTG CCTGCCTGGC TGCACTGCGG 
GAGTACATCA CGGACCCTGA GCGAGTGGTG CGGGAGAGCT GCGAGGTAGC ACTGGACATG 
TATGAGTATG AAAATGGCCA GGACTTCCAG TACGCAGATG GGCTCGAGCG CCTGAGGCCA 
CCGCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dohh ( XM_006240938.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006241000.1 Rattus norvegicus deoxyhypusine hydroxylase/monooxygenase (Dohh), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa07000
Clone ID Related Accession (Same CDS sequence)	XM_006240938.2 , NM_001025006.1 , XM_006240939.2 , XM_006240937.2
Accession Version	XM_006240938.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 909bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	deoxyhypusine hydroxylase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005106.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 7, 2014 this sequence version replaced XM_006240938.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTAACAG AACAGGAGGT AGAGGCCATA GGGAAAACCC TAGTGGACTC CACGCAGCCC 
CTGCAGGCCC GCTTCCGTGC CCTGTTTACC CTGCGGGGAC TTGGTGGCCC CGATGCTATC 
TCCTGGATCA GCCGAGGCTT TGAGGACAGC TCTGCCCTTC TGAAGCACGA GCTAGCCTAC 
TGCCTGGGCC AGATGAGGGA CCCACGAGCC ATCCCTGTGC TGGTGTCCGT CCTACAGGAC 
AGGAACCAGG AGCCCATGGT GCGTCATGAG GCAGGGGAAG CACTGGGGGC GATTGGTAAC 
CCGAAAGTTC TAGGCCTCCT GAAGCAGTAT TCCACCGACC CAGTGGTTGA GGTGGCTGAG 
ACGTGCCAGC TCGCCGTTAG GCGTCTAGAG TGGCTGCAGC AGCACCCGGG GGAGGCCACA 
TGTGCAGGAC CCTACCTCTC AGTAGATCCT GCACCCCCTG CGGCAGAAGG TGATGTGGGG 
CGGCTGCGGG AGACCCTGCT GGATGAGGCG CAGCCACTCT TCGAGCGGTA CCGTGCCATG 
TTTGCCCTGC GCAATGTGGG TGGCAAGGAG GCTGCCTTGG CCCTGGCTGA AGGCCTGAAG 
TGTGGCAGTG CGCTCTTCCG ACACGAGGTG GGCTATGTGC TGGGTCAGCT GCAGCACGAA 
GCAGCCGTGT CTGAACTGGC AGCCACATTG GCACGGACCA CTGAGAGCCC CATGGTACGA 
CATGAGTGCG CAGAGGCCCT GGGTGCCATC GCCAGGCCTG CCTGCCTGGC TGCACTGCGG 
GAGTACATCA CGGACCCTGA GCGAGTGGTG CGGGAGAGCT GCGAGGTAGC ACTGGACATG 
TATGAGTATG AAAATGGCCA GGACTTCCAG TACGCAGATG GGCTCGAGCG CCTGAGGCCA 
CCGCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006241000.1
CDS	409..1317
Translation	MVTEQEVEAIGKTLVDSTQPLQARFRALFTLRGLGGPDAISWISRGFEDSSALLKHELAYCLGQMRDPRAIPVLVSVLQDRNQEPMVRHEAGEALGAIGNPKVLGLLKQYSTDPVVEVAETCQLAVRRLEWLQQHPGEATCAGPYLSVDPAPPAAEGDVGRLRETLLDEAQPLFERYRAMFALRNVGGKEAALALAEGLKCGSALFRHEVGYVLGQLQHEAAVSELAATLARTTESPMVRHECAEALGAIARPACLAALREYITDPERVVRESCEVALDMYEYENGQDFQYADGLERLRPPP

Target ORF information:

RefSeq Version	XM_006240938.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus deoxyhypusine hydroxylase/monooxygenase (Dohh), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006240938.2

ORF Insert Sequence:

ATGGTAACAG AACAGGAGGT AGAGGCCATA GGGAAAACCC TAGTGGACTC CACGCAGCCC 
CTGCAGGCCC GCTTCCGTGC CCTGTTTACC CTGCGGGGAC TTGGTGGCCC CGATGCTATC 
TCCTGGATCA GCCGAGGCTT TGAGGACAGC TCTGCCCTTC TGAAGCACGA GCTAGCCTAC 
TGCCTGGGCC AGATGAGGGA CCCACGAGCC ATCCCTGTGC TGGTGTCCGT CCTACAGGAC 
AGGAACCAGG AGCCCATGGT GCGTCATGAG GCAGGGGAAG CACTGGGGGC GATTGGTAAC 
CCGAAAGTTC TAGGCCTCCT GAAGCAGTAT TCCACCGACC CAGTGGTTGA GGTGGCTGAG 
ACGTGCCAGC TCGCCGTTAG GCGTCTAGAG TGGCTGCAGC AGCACCCGGG GGAGGCCACA 
TGTGCAGGAC CCTACCTCTC AGTAGATCCT GCACCCCCTG CGGCAGAAGG TGATGTGGGG 
CGGCTGCGGG AGACCCTGCT GGATGAGGCG CAGCCACTCT TCGAGCGGTA CCGTGCCATG 
TTTGCCCTGC GCAATGTGGG TGGCAAGGAG GCTGCCTTGG CCCTGGCTGA AGGCCTGAAG 
TGTGGCAGTG CGCTCTTCCG ACACGAGGTG GGCTATGTGC TGGGTCAGCT GCAGCACGAA 
GCAGCCGTGT CTGAACTGGC AGCCACATTG GCACGGACCA CTGAGAGCCC CATGGTACGA 
CATGAGTGCG CAGAGGCCCT GGGTGCCATC GCCAGGCCTG CCTGCCTGGC TGCACTGCGG 
GAGTACATCA CGGACCCTGA GCGAGTGGTG CGGGAGAGCT GCGAGGTAGC ACTGGACATG 
TATGAGTATG AAAATGGCCA GGACTTCCAG TACGCAGATG GGCTCGAGCG CCTGAGGCCA 
CCGCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dohh ( XM_006240939.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006241001.1 Rattus norvegicus deoxyhypusine hydroxylase/monooxygenase (Dohh), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ORa07000
Clone ID Related Accession (Same CDS sequence)	XM_006240938.2 , NM_001025006.1 , XM_006240939.2 , XM_006240937.2
Accession Version	XM_006240939.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 909bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	deoxyhypusine hydroxylase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005106.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 7, 2014 this sequence version replaced XM_006240939.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTAACAG AACAGGAGGT AGAGGCCATA GGGAAAACCC TAGTGGACTC CACGCAGCCC 
CTGCAGGCCC GCTTCCGTGC CCTGTTTACC CTGCGGGGAC TTGGTGGCCC CGATGCTATC 
TCCTGGATCA GCCGAGGCTT TGAGGACAGC TCTGCCCTTC TGAAGCACGA GCTAGCCTAC 
TGCCTGGGCC AGATGAGGGA CCCACGAGCC ATCCCTGTGC TGGTGTCCGT CCTACAGGAC 
AGGAACCAGG AGCCCATGGT GCGTCATGAG GCAGGGGAAG CACTGGGGGC GATTGGTAAC 
CCGAAAGTTC TAGGCCTCCT GAAGCAGTAT TCCACCGACC CAGTGGTTGA GGTGGCTGAG 
ACGTGCCAGC TCGCCGTTAG GCGTCTAGAG TGGCTGCAGC AGCACCCGGG GGAGGCCACA 
TGTGCAGGAC CCTACCTCTC AGTAGATCCT GCACCCCCTG CGGCAGAAGG TGATGTGGGG 
CGGCTGCGGG AGACCCTGCT GGATGAGGCG CAGCCACTCT TCGAGCGGTA CCGTGCCATG 
TTTGCCCTGC GCAATGTGGG TGGCAAGGAG GCTGCCTTGG CCCTGGCTGA AGGCCTGAAG 
TGTGGCAGTG CGCTCTTCCG ACACGAGGTG GGCTATGTGC TGGGTCAGCT GCAGCACGAA 
GCAGCCGTGT CTGAACTGGC AGCCACATTG GCACGGACCA CTGAGAGCCC CATGGTACGA 
CATGAGTGCG CAGAGGCCCT GGGTGCCATC GCCAGGCCTG CCTGCCTGGC TGCACTGCGG 
GAGTACATCA CGGACCCTGA GCGAGTGGTG CGGGAGAGCT GCGAGGTAGC ACTGGACATG 
TATGAGTATG AAAATGGCCA GGACTTCCAG TACGCAGATG GGCTCGAGCG CCTGAGGCCA 
CCGCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006241001.1
CDS	406..1314
Translation	MVTEQEVEAIGKTLVDSTQPLQARFRALFTLRGLGGPDAISWISRGFEDSSALLKHELAYCLGQMRDPRAIPVLVSVLQDRNQEPMVRHEAGEALGAIGNPKVLGLLKQYSTDPVVEVAETCQLAVRRLEWLQQHPGEATCAGPYLSVDPAPPAAEGDVGRLRETLLDEAQPLFERYRAMFALRNVGGKEAALALAEGLKCGSALFRHEVGYVLGQLQHEAAVSELAATLARTTESPMVRHECAEALGAIARPACLAALREYITDPERVVRESCEVALDMYEYENGQDFQYADGLERLRPPP

Target ORF information:

RefSeq Version	XM_006240939.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus deoxyhypusine hydroxylase/monooxygenase (Dohh), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006240939.2

ORF Insert Sequence:

ATGGTAACAG AACAGGAGGT AGAGGCCATA GGGAAAACCC TAGTGGACTC CACGCAGCCC 
CTGCAGGCCC GCTTCCGTGC CCTGTTTACC CTGCGGGGAC TTGGTGGCCC CGATGCTATC 
TCCTGGATCA GCCGAGGCTT TGAGGACAGC TCTGCCCTTC TGAAGCACGA GCTAGCCTAC 
TGCCTGGGCC AGATGAGGGA CCCACGAGCC ATCCCTGTGC TGGTGTCCGT CCTACAGGAC 
AGGAACCAGG AGCCCATGGT GCGTCATGAG GCAGGGGAAG CACTGGGGGC GATTGGTAAC 
CCGAAAGTTC TAGGCCTCCT GAAGCAGTAT TCCACCGACC CAGTGGTTGA GGTGGCTGAG 
ACGTGCCAGC TCGCCGTTAG GCGTCTAGAG TGGCTGCAGC AGCACCCGGG GGAGGCCACA 
TGTGCAGGAC CCTACCTCTC AGTAGATCCT GCACCCCCTG CGGCAGAAGG TGATGTGGGG 
CGGCTGCGGG AGACCCTGCT GGATGAGGCG CAGCCACTCT TCGAGCGGTA CCGTGCCATG 
TTTGCCCTGC GCAATGTGGG TGGCAAGGAG GCTGCCTTGG CCCTGGCTGA AGGCCTGAAG 
TGTGGCAGTG CGCTCTTCCG ACACGAGGTG GGCTATGTGC TGGGTCAGCT GCAGCACGAA 
GCAGCCGTGT CTGAACTGGC AGCCACATTG GCACGGACCA CTGAGAGCCC CATGGTACGA 
CATGAGTGCG CAGAGGCCCT GGGTGCCATC GCCAGGCCTG CCTGCCTGGC TGCACTGCGG 
GAGTACATCA CGGACCCTGA GCGAGTGGTG CGGGAGAGCT GCGAGGTAGC ACTGGACATG 
TATGAGTATG AAAATGGCCA GGACTTCCAG TACGCAGATG GGCTCGAGCG CCTGAGGCCA 
CCGCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Dohh ( XM_006240937.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006240999.1 Rattus norvegicus deoxyhypusine hydroxylase/monooxygenase (Dohh), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa07000
Clone ID Related Accession (Same CDS sequence)	XM_006240938.2 , NM_001025006.1 , XM_006240939.2 , XM_006240937.2
Accession Version	XM_006240937.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 909bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	deoxyhypusine hydroxylase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005106.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 7, 2014 this sequence version replaced XM_006240937.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTAACAG AACAGGAGGT AGAGGCCATA GGGAAAACCC TAGTGGACTC CACGCAGCCC 
CTGCAGGCCC GCTTCCGTGC CCTGTTTACC CTGCGGGGAC TTGGTGGCCC CGATGCTATC 
TCCTGGATCA GCCGAGGCTT TGAGGACAGC TCTGCCCTTC TGAAGCACGA GCTAGCCTAC 
TGCCTGGGCC AGATGAGGGA CCCACGAGCC ATCCCTGTGC TGGTGTCCGT CCTACAGGAC 
AGGAACCAGG AGCCCATGGT GCGTCATGAG GCAGGGGAAG CACTGGGGGC GATTGGTAAC 
CCGAAAGTTC TAGGCCTCCT GAAGCAGTAT TCCACCGACC CAGTGGTTGA GGTGGCTGAG 
ACGTGCCAGC TCGCCGTTAG GCGTCTAGAG TGGCTGCAGC AGCACCCGGG GGAGGCCACA 
TGTGCAGGAC CCTACCTCTC AGTAGATCCT GCACCCCCTG CGGCAGAAGG TGATGTGGGG 
CGGCTGCGGG AGACCCTGCT GGATGAGGCG CAGCCACTCT TCGAGCGGTA CCGTGCCATG 
TTTGCCCTGC GCAATGTGGG TGGCAAGGAG GCTGCCTTGG CCCTGGCTGA AGGCCTGAAG 
TGTGGCAGTG CGCTCTTCCG ACACGAGGTG GGCTATGTGC TGGGTCAGCT GCAGCACGAA 
GCAGCCGTGT CTGAACTGGC AGCCACATTG GCACGGACCA CTGAGAGCCC CATGGTACGA 
CATGAGTGCG CAGAGGCCCT GGGTGCCATC GCCAGGCCTG CCTGCCTGGC TGCACTGCGG 
GAGTACATCA CGGACCCTGA GCGAGTGGTG CGGGAGAGCT GCGAGGTAGC ACTGGACATG 
TATGAGTATG AAAATGGCCA GGACTTCCAG TACGCAGATG GGCTCGAGCG CCTGAGGCCA 
CCGCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006240999.1
CDS	219..1127
Translation	MVTEQEVEAIGKTLVDSTQPLQARFRALFTLRGLGGPDAISWISRGFEDSSALLKHELAYCLGQMRDPRAIPVLVSVLQDRNQEPMVRHEAGEALGAIGNPKVLGLLKQYSTDPVVEVAETCQLAVRRLEWLQQHPGEATCAGPYLSVDPAPPAAEGDVGRLRETLLDEAQPLFERYRAMFALRNVGGKEAALALAEGLKCGSALFRHEVGYVLGQLQHEAAVSELAATLARTTESPMVRHECAEALGAIARPACLAALREYITDPERVVRESCEVALDMYEYENGQDFQYADGLERLRPPP

Target ORF information:

RefSeq Version	XM_006240937.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus deoxyhypusine hydroxylase/monooxygenase (Dohh), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006240937.2

ORF Insert Sequence:

ATGGTAACAG AACAGGAGGT AGAGGCCATA GGGAAAACCC TAGTGGACTC CACGCAGCCC 
CTGCAGGCCC GCTTCCGTGC CCTGTTTACC CTGCGGGGAC TTGGTGGCCC CGATGCTATC 
TCCTGGATCA GCCGAGGCTT TGAGGACAGC TCTGCCCTTC TGAAGCACGA GCTAGCCTAC 
TGCCTGGGCC AGATGAGGGA CCCACGAGCC ATCCCTGTGC TGGTGTCCGT CCTACAGGAC 
AGGAACCAGG AGCCCATGGT GCGTCATGAG GCAGGGGAAG CACTGGGGGC GATTGGTAAC 
CCGAAAGTTC TAGGCCTCCT GAAGCAGTAT TCCACCGACC CAGTGGTTGA GGTGGCTGAG 
ACGTGCCAGC TCGCCGTTAG GCGTCTAGAG TGGCTGCAGC AGCACCCGGG GGAGGCCACA 
TGTGCAGGAC CCTACCTCTC AGTAGATCCT GCACCCCCTG CGGCAGAAGG TGATGTGGGG 
CGGCTGCGGG AGACCCTGCT GGATGAGGCG CAGCCACTCT TCGAGCGGTA CCGTGCCATG 
TTTGCCCTGC GCAATGTGGG TGGCAAGGAG GCTGCCTTGG CCCTGGCTGA AGGCCTGAAG 
TGTGGCAGTG CGCTCTTCCG ACACGAGGTG GGCTATGTGC TGGGTCAGCT GCAGCACGAA 
GCAGCCGTGT CTGAACTGGC AGCCACATTG GCACGGACCA CTGAGAGCCC CATGGTACGA 
CATGAGTGCG CAGAGGCCCT GGGTGCCATC GCCAGGCCTG CCTGCCTGGC TGCACTGCGG 
GAGTACATCA CGGACCCTGA GCGAGTGGTG CGGGAGAGCT GCGAGGTAGC ACTGGACATG 
TATGAGTATG AAAATGGCCA GGACTTCCAG TACGCAGATG GGCTCGAGCG CCTGAGGCCA 
CCGCCCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Crystal Structure of the Peroxo-diiron(III) Intermediate of Deoxyhypusine Hydroxylase, an Oxygenase Involved in Hypusination.
Structure (London, England : 1993)23(5)882-892(2015 May)
Han Z,Sakai N,B?ttger LH,Klinke S,Hauber J,Trautwein AX,Hilgenfeld R

A novel mouse model for inhibition of DOHH-mediated hypusine modification reveals a crucial function in embryonic development, proliferation and oncogenic transformation.
Disease models & mechanisms7(8)963-76(2014 Aug)
Sievert H,P?llmann N,Miller KK,Hermans-Borgmeyer I,Venz S,Sendoel A,Preukschas M,Schweizer M,Boettcher S,Janiesch PC,Streichert T,Walther R,Hengartner MO,Manz MG,Br?mmendorf TH,Bokemeyer C,Braig M,Hauber J,Duncan KE,Balabanov S

Human deoxyhypusine hydroxylase, an enzyme involved in regulating cell growth, activates O2 with a nonheme diiron center.
Proceedings of the National Academy of Sciences of the United States of America106(35)14814-9(2009 Sep)
Vu VV,Emerson JP,Martinho M,Kim YS,M?nck E,Park MH,Que L Jr

Specificity of the deoxyhypusine hydroxylase-eukaryotic translation initiation factor (eIF5A) interaction: identification of amino acid residues of the enzyme required for binding of its substrate, deoxyhypusine-containing eIF5A.
The Journal of biological chemistry282(11)8300-8(2007 Mar)
Kang KR,Kim YS,Wolff EC,Park MH

Deoxyhypusine hydroxylase is a Fe(II)-dependent, HEAT-repeat enzyme. Identification of amino acid residues critical for Fe(II) binding and catalysis [corrected].
The Journal of biological chemistry281(19)13217-25(2006 May)
Kim YS,Kang KR,Wolff EC,Bell JK,McPhie P,Park MH

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Dohh cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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