Lpin1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Lpin1
Entrez Gene ID	313977
Full Name	lipin 1
General protein information	Preferred Names lipin 1 Names phosphatidate phosphatase LPIN1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 6 Map Location: 6q16

mRNA and Protein(s)

mRNA	Protein	Name
XM_008764593.2	XP_008762815.1	phosphatidate phosphatase LPIN1 isoform X1
NM_001012111.1	NP_001012111.1	phosphatidate phosphatase LPIN1

Pathways from BioSystems

Homologies

Homo sapiens (human)	LPIN1	NP_001248357.1
Rattus norvegicus (Norway rat)	Lpin1	NP_001012111.1
	LOC697682	XP_002808075.1
Gallus gallus (chicken)	LPIN1	XP_004935828.1
Pan troglodytes (chimpanzee)	LPIN1	XP_001160223.2
Mus musculus (house mouse)	Lpin1	NP_056578.2
Canis lupus familiaris (dog)	LPIN1	XP_532878.3
Bos taurus (cattle)	LPIN1	NP_001193085.1
Xenopus tropicalis (tropical clawed frog)	lpin1	NP_001072495.1
Danio rerio (zebrafish)	lpin1	NP_001037818.1

Gene Ontology
Bibliography

Related articles in PubMed

Obesity- and age-related alterations in FAT/CD36 translocation and lipin-1 subcellular localization in skeletal muscle of the Zucker rats.
Romic S, Krskova K, Olszanecki R, Balazova L, Lory V, Koricanac G, Slamkova M, Zorad S
General physiology and biophysics36(4)399-406(2017 Oct)

Gender differences in the expression and cellular localization of lipin 1 in the hearts of fructose-fed rats.
Romi? S, Tepav?evi? S, ?akula Z, Milosavljevi? T, Kosti? M, Petkovi? M, Kori?anac G
Lipids49(7)655-63(2014 Jul)

Differential regulation of the expressions of the PGC-1? splice variants, lipins, and PPAR? in heart compared to liver.
Kok BP, Dyck JR, Harris TE, Brindley DN
Journal of lipid research54(6)1662-77(2013 Jun)

Cell autonomous lipin 1 function is essential for development and maintenance of white and brown adipose tissue.
Nadra K, M?dard JJ, Mul JD, Han GS, Gr?s S, Pende M, Metzger D, Chambon P, Cuppen E, Saulnier-Blache JS, Carman GM, Desvergne B, Chrast R
Molecular and cellular biology32(23)4794-810(2012 Dec)

Aging of myelinating glial cells predominantly affects lipid metabolism and immune response pathways.
Verdier V, Cs?rdi G, de Preux-Charles AS, M?dard JJ, Smit AB, Verheijen MH, Bergmann S, Chrast R
Glia60(5)751-60(2012 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Increase in plasma membrane FAT/CD36 content in obese rats and failure in lipin-1 export to nucleus with progression of obesity, implying an increase in FA uptake and their different channeling into lipid intermediates synthesis pathway in old fa/fa rats versus FA usage in lean rats of the same age.
Title: Obesity- and age-related alterations in FAT/CD36 translocation and lipin-1 subcellular localization in skeletal muscle of the Zucker rats.

Total expression of lipin 1 in cardiac cell lysate and its cytosolic and microsomal level were increased in the hearts of male fructose-fed rats(FFR).
Title: Gender differences in the expression and cellular localization of lipin 1 in the hearts of fructose-fed rats.

novel differences in the regulation of lipins 1,2,3, PPARalpha, and PGC-1alpha splice variants during fasting in heart versus liver, even though the ultimate outcome in both tissues is to increase FA turnover and oxidation.
Title: Differential regulation of the expressions of the PGC-1? splice variants, lipins, and PPAR? in heart compared to liver.

The decrease in lipin1 expression and AMPKalpha activation may contribute to hepatic insulin resistance in diet-induced obese rats.
Title: Expression and significance of lipin1 and AMPK? in hepatic insulin resistance in diet-induced insulin resistance rats.

Estrogen-related receptor gamma (ERRgamma) is a novel transcriptional regulator of phosphatidic acid phosphatase, LIPIN1, and inhibits hepatic insulin signaling
Title: Estrogen-related receptor ? (ERR?) is a novel transcriptional regulator of phosphatidic acid phosphatase, LIPIN1, and inhibits hepatic insulin signaling.

The following Lpin1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Lpin1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa02337	NM_001012111.1 Latest version!	Rattus norvegicus lipin 1 (Lpin1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$475.30 $679.00
ORa47290	XM_008764593.2 Latest version!	Rattus norvegicus lipin 1 (Lpin1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Lpin1 ( NM_001012111.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001012111.1 Rattus norvegicus lipin 1 (Lpin1), mRNA.

Price & Availability↑

CloneID	ORa02337
Clone ID Related Accession (Same CDS sequence)	NM_001012111.1
Accession Version	NM_001012111.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2775bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-18
Organism	Rattus norvegicus(Norway rat)
Product	phosphatidate phosphatase LPIN1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC083651.1. On Feb 11, 2005 this sequence version replaced XM_243142.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC083651.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGAATTACG TGGGGCAGTT GGCCGGCCAG GTGTTTGTGA CGGTGAAGGA GCTCTACAAG 
GGACTGAACC CTGCCACGCT GTCAGGATGC ATCGACATCA TTGTTGTCCG CCAGCCCAAT 
GGCAGTCTGC AGTGCTCCCC GTTCCACGTC CGCTTCGGGA AGATGGGTGT CCTACGCTCC 
CGAGAGAAAG TGGTGGACAT AGAAATCAAT GGGGAGTCTG TGGATTTGCA CATGAAGTTG 
GGAGACAACG GAGAAGCGTT TTTTGTTCAA GAGACTGACA ACGATCAGGA AGTCATCCCC 
ATGTACCTAG CCACGTCCCC CATCCTGTCA GAAGGAGCTT CGAGAATGGA AAGCCAGCTG 
AAAAGGAACT CTGTGGACAG AATCCGGGGC CTGGATCCCA CTACAGCTGC CCAGGGCCTG 
CCTCCCAGCG ACACCCCATC CACTGGTTCT CTGGGGAAGA AGAGAAGGAA AAGGAGGAGG 
AAGGCCCATT TGGACAATCT CAAAAGAGAT GACAATGTCA ACACATCTGA GGACGAGGAC 
ATGTTTCCCA TAGAGATGAG CTCGGATGAG GACACAGCAC TGATGGATGG GAGCAGAGCT 
CTTCCTAATG ATGTACCACC ATTCCAAGAT GATATTCCTA AGGAAAACCT CCCCTCGATT 
TCAACATATC CCCAGTCAGC ATCGTACCCC AATTCAGACA GAGAGTGGTC CCCCAGCCCC 
AGCAGCCTGG TAGATTGTCA GAGGACTCCC CCTCACCTGG CCGAGGGAGT TCTCTCTAGC 
TCTTGTCCTC TGCAGTCTTG CCACTTCCAT GCTTCGGAAA GTCCTTCAGG CTCCCGGCCC 
TCAACACCAA AAAGTGATTC TGAGCTGGTC AGTAAGTCTG CAGACAGATT GACGCCAAAG 
AATAACCTGG AAATGCTCTG GCTATGGGGT GAATTGCCAC AGGCTGCAAA GTCGTCTTCT 
CCACACAAGA TGAAAGACTC CAGCCCCTTA GGAGGTCGGA AGATGGCTGA TAAAATCCAC 
TTCCAGACCA TTCACAGCGA GTCTTCAGAC ACTTTCAGTG ACCAGTCCCC AACAATGGCC 
CGGGGGCTGC TCATCCACCA GAGCAAGGCT CAGACGGAAA TGCAGTTTGT GAATGAGGAG 
GACCTTGAGG CCTTGGGGGC CGCAGCCCCA CCTTTGTCTG TAGCCGAAGA GCTCAAGGCC 
CCAGCCTCCA GCACAGCACA GACATCAAGC AAGTCAGATT CCCCTTCCAG AAAAAAAGAT 
AAACGAAGCC GGCACCTCGG AGCTGATGGC GTTTATCTGG ATGACCTCAC AGACATGGAC 
CCGGAAGTGG CTGCCCTGTA TTTCCCCAAG AATGGGGATC CCGGTGGGCT TCCCAAACAA 
GCGAGTGACA ACGGAGCCAG GTCAGCCAAC CAGTCCCCAC AGTCGGTGGG TAGCTCGGGC 
ATTGACAGCG GCGTGGAGAG CACCTCCGAC AGCCTGAGGG ACCTGCCGTC CATCGCCATC 
TCCCTCTGCG GTGGCCTCAG TGACCACAGA GAGATCACCA AAGATGCATT TTTGGAACAA 
GCCGTGTCAT ATCAGCAATT TGCCGACAAC CCTGCTATCA TCGATGATCC CAACCTCGTG 
GTCAAGATTG GTAATAAGTA CTACAACTGG ACAACAGCAG CTCCTCTACT TCTGGCGATG 
CAGGCTTTCC AGAAACCTTT GCCAAAGGCC ACTGTGGAAT CCATCATGAG AGATAAGATG 
CCCAAAAAGG GAGGGAGATG GTGGTTTTCC TGGAGAGGAA GAAACGCCAC AATCAAAGAG 
GAGAGCAAGC CTGAGCAGGG CCTGCCTGGG AAGGGCCACA ATACCGGAGA GCAGCCTGCA 
CAGCTTGGCC TGACCACCAG AATAAAGCAT GAGTCATCCT CTAGTGATGA AGAGCACTCA 
GCTACCAAGC CATCGAGCTC GAGCCACCTC TCTCTCTTGT CCAACGTCAG CTACAAAAAG 
ACCCTGCGGC TCACATCGGA GCAGTTGAAA AGCTTGAAGT TGAAGAATGG CCCCAACGAT 
GTGGTGTTCA GTGTCACTAC CCAGTACCAG GGCACCTGTC GCTGTGAGGG TACCATCTAC 
CTGTGGAATT GGGATGACAA AGTCATCATC TCAGATATCG ATGGTACCAT CACAAGATCT 
GATACTCTTG GTCACATATT GCCCACACTG GGAAAGGATT GGACTCACCA GGGCATCGCA 
AAGCTGTATC ACAAAGTAAG CCAGAATGGC TACAAGTTTC TCTATTGTTC TGCACGTGCC 
ATTGGGATGG CAGACATGAC GAGGGGCTAT CTGCACTGGG TCAACGAGAG GGGCACAGTG 
CTGCCACAGG GGCCGCTTCT GCTGAGCCCC AGCAGCCTCT TCTCCGCCTT GCACAGAGAA 
GTGATTGAAA AGAAGCCAGA AAAGTTCAAA GTCCAGTGTT TGACAGACAT CAAAAACCTG 
TTTTTCCCAA ACACAGAGCC CTTTTATGCT GCTTTTGGGA ACCGGCCTGC CGATGTGTAT 
TCCTACAAGC AAGTGGGAGT GTCCCTGAAT AGAATCTTCA CCGTCAACCC CAAGGGTGAG 
CTGGTGCAGG AGCATGCCAA GACCAACATC AGCTCGTATG TGCGGCTCTG CGAAGTGGTC 
GACCACGTCT TCCCCTTGCT GAAGAGAAGC CATTCCTGCG ACTTCCCCTG TTCAGACACT 
TTCAGTAACT TCACCTTTTG GAGAGAGCCA CTGCCACCCT TTGAAAACCA GGACGTGCAT 
TCAGCCTCAG CTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001012111.1
CDS	269..3043
Translation	MNYVGQLAGQVFVTVKELYKGLNPATLSGCIDIIVVRQPNGSLQCSPFHVRFGKMGVLRSREKVVDIEINGESVDLHMKLGDNGEAFFVQETDNDQEVIPMYLATSPILSEGASRMESQLKRNSVDRIRGLDPTTAAQGLPPSDTPSTGSLGKKRRKRRRKAHLDNLKRDDNVNTSEDEDMFPIEMSSDEDTALMDGSRALPNDVPPFQDDIPKENLPSISTYPQSASYPNSDREWSPSPSSLVDCQRTPPHLAEGVLSSSCPLQSCHFHASESPSGSRPSTPKSDSELVSKSADRLTPKNNLEMLWLWGELPQAAKSSSPHKMKDSSPLGGRKMADKIHFQTIHSESSDTFSDQSPTMARGLLIHQSKAQTEMQFVNEEDLEALGAAAPPLSVAEELKAPASSTAQTSSKSDSPSRKKDKRSRHLGADGVYLDDLTDMDPEVAALYFPKNGDPGGLPKQASDNGARSANQSPQSVGSSGIDSGVESTSDSLRDLPSIAISLCGGLSDHREITKDAFLEQAVSYQQFADNPAIIDDPNLVVKIGNKYYNWTTAAPLLLAMQAFQKPLPKATVESIMRDKMPKKGGRWWFSWRGRNATIKEESKPEQGLPGKGHNTGEQPAQLGLTTRIKHESSSSDEEHSATKPSSSSHLSLLSNVSYKKTLRLTSEQLKSLKLKNGPNDVVFSVTTQYQGTCRCEGTIYLWNWDDKVIISDIDGTITRSDTLGHILPTLGKDWTHQGIAKLYHKVSQNGYKFLYCSARAIGMADMTRGYLHWVNERGTVLPQGPLLLSPSSLFSALHREVIEKKPEKFKVQCLTDIKNLFFPNTEPFYAAFGNRPADVYSYKQVGVSLNRIFTVNPKGELVQEHAKTNISSYVRLCEVVDHVFPLLKRSHSCDFPCSDTFSNFTFWREPLPPFENQDVHSASA

Target ORF information:

RefSeq Version	NM_001012111.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus lipin 1 (Lpin1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001012111.1

ORF Insert Sequence:

ATGAATTACG TGGGGCAGTT GGCCGGCCAG GTGTTTGTGA CGGTGAAGGA GCTCTACAAG 
GGACTGAACC CTGCCACGCT GTCAGGATGC ATCGACATCA TTGTTGTCCG CCAGCCCAAT 
GGCAGTCTGC AGTGCTCCCC GTTCCACGTC CGCTTCGGGA AGATGGGTGT CCTACGCTCC 
CGAGAGAAAG TGGTGGACAT AGAAATCAAT GGGGAGTCTG TGGATTTGCA CATGAAGTTG 
GGAGACAACG GAGAAGCGTT TTTTGTTCAA GAGACTGACA ACGATCAGGA AGTCATCCCC 
ATGTACCTAG CCACGTCCCC CATCCTGTCA GAAGGAGCTT CGAGAATGGA AAGCCAGCTG 
AAAAGGAACT CTGTGGACAG AATCCGGGGC CTGGATCCCA CTACAGCTGC CCAGGGCCTG 
CCTCCCAGCG ACACCCCATC CACTGGTTCT CTGGGGAAGA AGAGAAGGAA AAGGAGGAGG 
AAGGCCCATT TGGACAATCT CAAAAGAGAT GACAATGTCA ACACATCTGA GGACGAGGAC 
ATGTTTCCCA TAGAGATGAG CTCGGATGAG GACACAGCAC TGATGGATGG GAGCAGAGCT 
CTTCCTAATG ATGTACCACC ATTCCAAGAT GATATTCCTA AGGAAAACCT CCCCTCGATT 
TCAACATATC CCCAGTCAGC ATCGTACCCC AATTCAGACA GAGAGTGGTC CCCCAGCCCC 
AGCAGCCTGG TAGATTGTCA GAGGACTCCC CCTCACCTGG CCGAGGGAGT TCTCTCTAGC 
TCTTGTCCTC TGCAGTCTTG CCACTTCCAT GCTTCGGAAA GTCCTTCAGG CTCCCGGCCC 
TCAACACCAA AAAGTGATTC TGAGCTGGTC AGTAAGTCTG CAGACAGATT GACGCCAAAG 
AATAACCTGG AAATGCTCTG GCTATGGGGT GAATTGCCAC AGGCTGCAAA GTCGTCTTCT 
CCACACAAGA TGAAAGACTC CAGCCCCTTA GGAGGTCGGA AGATGGCTGA TAAAATCCAC 
TTCCAGACCA TTCACAGCGA GTCTTCAGAC ACTTTCAGTG ACCAGTCCCC AACAATGGCC 
CGGGGGCTGC TCATCCACCA GAGCAAGGCT CAGACGGAAA TGCAGTTTGT GAATGAGGAG 
GACCTTGAGG CCTTGGGGGC CGCAGCCCCA CCTTTGTCTG TAGCCGAAGA GCTCAAGGCC 
CCAGCCTCCA GCACAGCACA GACATCAAGC AAGTCAGATT CCCCTTCCAG AAAAAAAGAT 
AAACGAAGCC GGCACCTCGG AGCTGATGGC GTTTATCTGG ATGACCTCAC AGACATGGAC 
CCGGAAGTGG CTGCCCTGTA TTTCCCCAAG AATGGGGATC CCGGTGGGCT TCCCAAACAA 
GCGAGTGACA ACGGAGCCAG GTCAGCCAAC CAGTCCCCAC AGTCGGTGGG TAGCTCGGGC 
ATTGACAGCG GCGTGGAGAG CACCTCCGAC AGCCTGAGGG ACCTGCCGTC CATCGCCATC 
TCCCTCTGCG GTGGCCTCAG TGACCACAGA GAGATCACCA AAGATGCATT TTTGGAACAA 
GCCGTGTCAT ATCAGCAATT TGCCGACAAC CCTGCTATCA TCGATGATCC CAACCTCGTG 
GTCAAGATTG GTAATAAGTA CTACAACTGG ACAACAGCAG CTCCTCTACT TCTGGCGATG 
CAGGCTTTCC AGAAACCTTT GCCAAAGGCC ACTGTGGAAT CCATCATGAG AGATAAGATG 
CCCAAAAAGG GAGGGAGATG GTGGTTTTCC TGGAGAGGAA GAAACGCCAC AATCAAAGAG 
GAGAGCAAGC CTGAGCAGGG CCTGCCTGGG AAGGGCCACA ATACCGGAGA GCAGCCTGCA 
CAGCTTGGCC TGACCACCAG AATAAAGCAT GAGTCATCCT CTAGTGATGA AGAGCACTCA 
GCTACCAAGC CATCGAGCTC GAGCCACCTC TCTCTCTTGT CCAACGTCAG CTACAAAAAG 
ACCCTGCGGC TCACATCGGA GCAGTTGAAA AGCTTGAAGT TGAAGAATGG CCCCAACGAT 
GTGGTGTTCA GTGTCACTAC CCAGTACCAG GGCACCTGTC GCTGTGAGGG TACCATCTAC 
CTGTGGAATT GGGATGACAA AGTCATCATC TCAGATATCG ATGGTACCAT CACAAGATCT 
GATACTCTTG GTCACATATT GCCCACACTG GGAAAGGATT GGACTCACCA GGGCATCGCA 
AAGCTGTATC ACAAAGTAAG CCAGAATGGC TACAAGTTTC TCTATTGTTC TGCACGTGCC 
ATTGGGATGG CAGACATGAC GAGGGGCTAT CTGCACTGGG TCAACGAGAG GGGCACAGTG 
CTGCCACAGG GGCCGCTTCT GCTGAGCCCC AGCAGCCTCT TCTCCGCCTT GCACAGAGAA 
GTGATTGAAA AGAAGCCAGA AAAGTTCAAA GTCCAGTGTT TGACAGACAT CAAAAACCTG 
TTTTTCCCAA ACACAGAGCC CTTTTATGCT GCTTTTGGGA ACCGGCCTGC CGATGTGTAT 
TCCTACAAGC AAGTGGGAGT GTCCCTGAAT AGAATCTTCA CCGTCAACCC CAAGGGTGAG 
CTGGTGCAGG AGCATGCCAA GACCAACATC AGCTCGTATG TGCGGCTCTG CGAAGTGGTC 
GACCACGTCT TCCCCTTGCT GAAGAGAAGC CATTCCTGCG ACTTCCCCTG TTCAGACACT 
TTCAGTAACT TCACCTTTTG GAGAGAGCCA CTGCCACCCT TTGAAAACCA GGACGTGCAT 
TCAGCCTCAG CTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Lpin1 ( XM_008764593.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008762815.1 Rattus norvegicus lipin 1 (Lpin1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa47290
Clone ID Related Accession (Same CDS sequence)	XM_008764593.2
Accession Version	XM_008764593.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2922bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	phosphatidate phosphatase LPIN1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005105.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008764593.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGAGA AGGATCCTGT CTGCAGCTCC AAGTGGGAGG CACCACGGGA CAAGAGCTCC 
CTGGACTCGG CATGGTCCTG GATTCCAATA ATGAGAGACC CTGGATGGAT CCGAAACGTG 
TGGCCTTCAA ACATCAATGT TCAGACAATG AATTACGTGG GGCAGTTGGC CGGCCAGGTG 
TTTGTGACGG TGAAGGAGCT CTACAAGGGA CTGAACCCTG CCACGCTGTC AGGATGCATC 
GACATCATTG TTGTCCGCCA GCCCAATGGC AGTCTGCAGT GCTCCCCGTT CCACGTCCGC 
TTCGGGAAGA TGGGTGTCCT ACGCTCCCGA GAGAAAGTGG TGGACATAGA AATCAATGGG 
GAGTCTGTGG ATTTGCACAT GAAGTTGGGA GACAACGGAG AAGCGTTTTT TGTTCAAGAG 
ACTGACAACG ATCAGGAAGT CATCCCCATG TACCTAGCCA CGTCCCCCAT CCTGTCAGAA 
GGAGCTTCGA GAATGGAAAG CCAGCTGAAA AGGAACTCTG TGGACAGAAT CCGGGGCCTG 
GATCCCACTA CAGCTGCCCA GGGCCTGCCT CCCAGCGACA CCCCATCCAC TGGTTCTCTG 
GGGAAGAAGA GAAGGAAAAG GAGGAGGAAG GCCCATTTGG ACAATCTCAA AAGAGATGAC 
AATGTCAACA CATCTGAGGA CGAGGACATG TTTCCCATAG AGATGAGCTC GGATGAGGAC 
ACAGCACTGA TGGATTCCTC TAGAGCTCTT CCTAATGATG TACCACCATT CCAAGATGAT 
ATTCCTAAGG AAAACCTCCC CTCGATTTCA ACATATCCCC AGTCAGCATC GTACCCCAAT 
TCAGACAGAG AGTGGTCCCC CAGCCCCAGC AGCCTGGTAG ATTGTCAGAG GACTCCCCCT 
CACCTGGCCG AGGGAGTTCT CTCTAGCTCT TGTCCTCTGC AGTCTTGCCA CTTCCATGCT 
TCGGAAAGTC CTTCAGGCTC CCGGCCCTCA ACACCAAAAA GTGATTCTGA GCTGGTCAGT 
AAGTCTGCAG ACAGATTGAC GCCAAAGAAT AACCTGGAAA TGCTCTGGCT ATGGGGTGAA 
TTGCCACAGG CTGCAAAGTC GTCTTCTCCA CACAAGATGA AAGACTCCAG CCCCTTAGGA 
GGTCGGAAGA TGGCTGATAA AATCCACTTC CAGACCATTC ACAGCGAGTC TTCAGACACT 
TTCAGTGACC AGTCCCCAAC AATGGCCCGG GGGCTGCTCA TCCACCAGAG CAAGGCTCAG 
ACGGAAATGC AGTTTGTGAA TGAGGAGGAC CTTGAGGCCT TGGGGGCCGC AGCCCCACCT 
TTGTCTGTAG CCGAAGAGCT CAAGGCCCCA GCCTCCAGCA CAGCACAGAC ATCAAGCAAG 
TCAGATTCCC CTTCCAGAAA AAAAGATAAA CGAAGCCGGC ACCTCGGAGC TGATGGCGTT 
TATCTGGATG ACCTCACAGA CATGGACCCG GAAGTGGCTG CCCTGTATTT CCCCAAGAAT 
GGGGATCCCG GTGGGCTTCC CAAACAAGCG AGTGACAACG GAGCCAGGTC AGCCAACCAG 
TCCCCACAGT CGGTGGGTAG CTCGGGCATT GACAGCGGCG TGGAGAGCAC CTCCGACAGC 
CTGAGGGACC TGCCGTCCAT CGCCATCTCC CTCTGCGGTG GCCTCAGTGA CCACAGAGAG 
ATCACCAAAG ATGCATTTTT GGAACAAGCC GTGTCATATC AGCAATTTGC CGACAACCCT 
GCTATCATCG ATGATCCCAA CCTCGTGGTC AAGATTGGTA ATAAGTACTA CAACTGGACA 
ACAGCAGCTC CTCTACTTCT GGCGATGCAG GCTTTCCAGA AACCTTTGCC AAAGGCCACT 
GTGGAATCCA TCATGAGAGA TAAGATGCCC AAAAAGGGAG GGAGATGGTG GTTTTCCTGG 
AGAGGAAGAA ACGCCACAAT CAAAGAGGAG AGCAAGCCTG AGCAGGGCCT GCCTGGGAAG 
GGCCACAATA CCGGAGAGCA GCCTGCACAG CTTGGCCTGA CCACCAGAAT AAAGCATGAG 
TCATCCTCTA GTGATGAAGA GCACTCAGCT ACCAAGCCAT CGAGCTCGAG CCACCTCTCT 
CTCTTGTCCA ACGTCAGCTA CAAAAAGACC CTGCGGCTCA CATCGGAGCA GTTGAAAAGC 
TTGAAGTTGA AGAATGGCCC CAACGATGTG GTGTTCAGTG TCACTACCCA GTACCAGGGC 
ACCTGTCGCT GTGAGGGTAC CATCTACCTG TGGAATTGGG ATGACAAAGT CATCATCTCA 
GATATCGATG GTACCATCAC AAGATCTGAT ACTCTTGGTC ACATATTGCC CACACTGGGA 
AAGGATTGGA CTCACCAGGG CATCGCAAAG CTGTATCACA AAGTAAGCCA GAATGGCTAC 
AAGTTTCTCT ATTGTTCTGC ACGTGCCATT GGGATGGCAG ACATGACGAG GGGCTATCTG 
CACTGGGTCA ACGAGAGGGG CACAGTGCTG CCACAGGGGC CGCTTCTGCT GAGCCCCAGC 
AGCCTCTTCT CCGCCTTGCA CAGAGAAGTG ATTGAAAAGA AGCCAGAAAA GTTCAAAGTC 
CAGTGTTTGA CAGACATCAA AAACCTGTTT TTCCCAAACA CAGAGCCCTT TTATGCTGCT 
TTTGGGAACC GGCCTGCCGA TGTGTATTCC TACAAGCAAG TGGGAGTGTC CCTGAATAGA 
ATCTTCACCG TCAACCCCAA GGGTGAGCTG GTGCAGGAGC ATGCCAAGAC CAACATCAGC 
TCGTATGTGC GGCTCTGCGA AGTGGTCGAC CACGTCTTCC CCTTGCTGAA GAGAAGCCAT 
TCCTGCGACT TCCCCTGTTC AGACACTTTC AGTAACTTCA CCTTTTGGAG AGAGCCACTG 
CCACCCTTTG AAAACCAGGA CGTGCATTCA GCCTCAGCTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008762815.1
CDS	86..3007
Translation	MAEKDPVCSSKWEAPRDKSSLDSAWSWIPIMRDPGWIRNVWPSNINVQTMNYVGQLAGQVFVTVKELYKGLNPATLSGCIDIIVVRQPNGSLQCSPFHVRFGKMGVLRSREKVVDIEINGESVDLHMKLGDNGEAFFVQETDNDQEVIPMYLATSPILSEGASRMESQLKRNSVDRIRGLDPTTAAQGLPPSDTPSTGSLGKKRRKRRRKAHLDNLKRDDNVNTSEDEDMFPIEMSSDEDTALMDSSRALPNDVPPFQDDIPKENLPSISTYPQSASYPNSDREWSPSPSSLVDCQRTPPHLAEGVLSSSCPLQSCHFHASESPSGSRPSTPKSDSELVSKSADRLTPKNNLEMLWLWGELPQAAKSSSPHKMKDSSPLGGRKMADKIHFQTIHSESSDTFSDQSPTMARGLLIHQSKAQTEMQFVNEEDLEALGAAAPPLSVAEELKAPASSTAQTSSKSDSPSRKKDKRSRHLGADGVYLDDLTDMDPEVAALYFPKNGDPGGLPKQASDNGARSANQSPQSVGSSGIDSGVESTSDSLRDLPSIAISLCGGLSDHREITKDAFLEQAVSYQQFADNPAIIDDPNLVVKIGNKYYNWTTAAPLLLAMQAFQKPLPKATVESIMRDKMPKKGGRWWFSWRGRNATIKEESKPEQGLPGKGHNTGEQPAQLGLTTRIKHESSSSDEEHSATKPSSSSHLSLLSNVSYKKTLRLTSEQLKSLKLKNGPNDVVFSVTTQYQGTCRCEGTIYLWNWDDKVIISDIDGTITRSDTLGHILPTLGKDWTHQGIAKLYHKVSQNGYKFLYCSARAIGMADMTRGYLHWVNERGTVLPQGPLLLSPSSLFSALHREVIEKKPEKFKVQCLTDIKNLFFPNTEPFYAAFGNRPADVYSYKQVGVSLNRIFTVNPKGELVQEHAKTNISSYVRLCEVVDHVFPLLKRSHSCDFPCSDTFSNFTFWREPLPPFENQDVHSASA

Target ORF information:

RefSeq Version	XM_008764593.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus lipin 1 (Lpin1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008764593.2

ORF Insert Sequence:

ATGGCAGAGA AGGATCCTGT CTGCAGCTCC AAGTGGGAGG CACCACGGGA CAAGAGCTCC 
CTGGACTCGG CATGGTCCTG GATTCCAATA ATGAGAGACC CTGGATGGAT CCGAAACGTG 
TGGCCTTCAA ACATCAATGT TCAGACAATG AATTACGTGG GGCAGTTGGC CGGCCAGGTG 
TTTGTGACGG TGAAGGAGCT CTACAAGGGA CTGAACCCTG CCACGCTGTC AGGATGCATC 
GACATCATTG TTGTCCGCCA GCCCAATGGC AGTCTGCAGT GCTCCCCGTT CCACGTCCGC 
TTCGGGAAGA TGGGTGTCCT ACGCTCCCGA GAGAAAGTGG TGGACATAGA AATCAATGGG 
GAGTCTGTGG ATTTGCACAT GAAGTTGGGA GACAACGGAG AAGCGTTTTT TGTTCAAGAG 
ACTGACAACG ATCAGGAAGT CATCCCCATG TACCTAGCCA CGTCCCCCAT CCTGTCAGAA 
GGAGCTTCGA GAATGGAAAG CCAGCTGAAA AGGAACTCTG TGGACAGAAT CCGGGGCCTG 
GATCCCACTA CAGCTGCCCA GGGCCTGCCT CCCAGCGACA CCCCATCCAC TGGTTCTCTG 
GGGAAGAAGA GAAGGAAAAG GAGGAGGAAG GCCCATTTGG ACAATCTCAA AAGAGATGAC 
AATGTCAACA CATCTGAGGA CGAGGACATG TTTCCCATAG AGATGAGCTC GGATGAGGAC 
ACAGCACTGA TGGATTCCTC TAGAGCTCTT CCTAATGATG TACCACCATT CCAAGATGAT 
ATTCCTAAGG AAAACCTCCC CTCGATTTCA ACATATCCCC AGTCAGCATC GTACCCCAAT 
TCAGACAGAG AGTGGTCCCC CAGCCCCAGC AGCCTGGTAG ATTGTCAGAG GACTCCCCCT 
CACCTGGCCG AGGGAGTTCT CTCTAGCTCT TGTCCTCTGC AGTCTTGCCA CTTCCATGCT 
TCGGAAAGTC CTTCAGGCTC CCGGCCCTCA ACACCAAAAA GTGATTCTGA GCTGGTCAGT 
AAGTCTGCAG ACAGATTGAC GCCAAAGAAT AACCTGGAAA TGCTCTGGCT ATGGGGTGAA 
TTGCCACAGG CTGCAAAGTC GTCTTCTCCA CACAAGATGA AAGACTCCAG CCCCTTAGGA 
GGTCGGAAGA TGGCTGATAA AATCCACTTC CAGACCATTC ACAGCGAGTC TTCAGACACT 
TTCAGTGACC AGTCCCCAAC AATGGCCCGG GGGCTGCTCA TCCACCAGAG CAAGGCTCAG 
ACGGAAATGC AGTTTGTGAA TGAGGAGGAC CTTGAGGCCT TGGGGGCCGC AGCCCCACCT 
TTGTCTGTAG CCGAAGAGCT CAAGGCCCCA GCCTCCAGCA CAGCACAGAC ATCAAGCAAG 
TCAGATTCCC CTTCCAGAAA AAAAGATAAA CGAAGCCGGC ACCTCGGAGC TGATGGCGTT 
TATCTGGATG ACCTCACAGA CATGGACCCG GAAGTGGCTG CCCTGTATTT CCCCAAGAAT 
GGGGATCCCG GTGGGCTTCC CAAACAAGCG AGTGACAACG GAGCCAGGTC AGCCAACCAG 
TCCCCACAGT CGGTGGGTAG CTCGGGCATT GACAGCGGCG TGGAGAGCAC CTCCGACAGC 
CTGAGGGACC TGCCGTCCAT CGCCATCTCC CTCTGCGGTG GCCTCAGTGA CCACAGAGAG 
ATCACCAAAG ATGCATTTTT GGAACAAGCC GTGTCATATC AGCAATTTGC CGACAACCCT 
GCTATCATCG ATGATCCCAA CCTCGTGGTC AAGATTGGTA ATAAGTACTA CAACTGGACA 
ACAGCAGCTC CTCTACTTCT GGCGATGCAG GCTTTCCAGA AACCTTTGCC AAAGGCCACT 
GTGGAATCCA TCATGAGAGA TAAGATGCCC AAAAAGGGAG GGAGATGGTG GTTTTCCTGG 
AGAGGAAGAA ACGCCACAAT CAAAGAGGAG AGCAAGCCTG AGCAGGGCCT GCCTGGGAAG 
GGCCACAATA CCGGAGAGCA GCCTGCACAG CTTGGCCTGA CCACCAGAAT AAAGCATGAG 
TCATCCTCTA GTGATGAAGA GCACTCAGCT ACCAAGCCAT CGAGCTCGAG CCACCTCTCT 
CTCTTGTCCA ACGTCAGCTA CAAAAAGACC CTGCGGCTCA CATCGGAGCA GTTGAAAAGC 
TTGAAGTTGA AGAATGGCCC CAACGATGTG GTGTTCAGTG TCACTACCCA GTACCAGGGC 
ACCTGTCGCT GTGAGGGTAC CATCTACCTG TGGAATTGGG ATGACAAAGT CATCATCTCA 
GATATCGATG GTACCATCAC AAGATCTGAT ACTCTTGGTC ACATATTGCC CACACTGGGA 
AAGGATTGGA CTCACCAGGG CATCGCAAAG CTGTATCACA AAGTAAGCCA GAATGGCTAC 
AAGTTTCTCT ATTGTTCTGC ACGTGCCATT GGGATGGCAG ACATGACGAG GGGCTATCTG 
CACTGGGTCA ACGAGAGGGG CACAGTGCTG CCACAGGGGC CGCTTCTGCT GAGCCCCAGC 
AGCCTCTTCT CCGCCTTGCA CAGAGAAGTG ATTGAAAAGA AGCCAGAAAA GTTCAAAGTC 
CAGTGTTTGA CAGACATCAA AAACCTGTTT TTCCCAAACA CAGAGCCCTT TTATGCTGCT 
TTTGGGAACC GGCCTGCCGA TGTGTATTCC TACAAGCAAG TGGGAGTGTC CCTGAATAGA 
ATCTTCACCG TCAACCCCAA GGGTGAGCTG GTGCAGGAGC ATGCCAAGAC CAACATCAGC 
TCGTATGTGC GGCTCTGCGA AGTGGTCGAC CACGTCTTCC CCTTGCTGAA GAGAAGCCAT 
TCCTGCGACT TCCCCTGTTC AGACACTTTC AGTAACTTCA CCTTTTGGAG AGAGCCACTG 
CCACCCTTTG AAAACCAGGA CGTGCATTCA GCCTCAGCTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Obesity- and age-related alterations in FAT/CD36 translocation and lipin-1 subcellular localization in skeletal muscle of the Zucker rats.
General physiology and biophysics36(4)399-406(2017 Oct)
Romic S,Krskova K,Olszanecki R,Balazova L,Lory V,Koricanac G,Slamkova M,Zorad S

Gender differences in the expression and cellular localization of lipin 1 in the hearts of fructose-fed rats.
Lipids49(7)655-63(2014 Jul)
Romi? S,Tepav?evi? S,?akula Z,Milosavljevi? T,Kosti? M,Petkovi? M,Kori?anac G

Differential regulation of the expressions of the PGC-1? splice variants, lipins, and PPAR? in heart compared to liver.
Journal of lipid research54(6)1662-77(2013 Jun)
Kok BP,Dyck JR,Harris TE,Brindley DN

Cell autonomous lipin 1 function is essential for development and maintenance of white and brown adipose tissue.
Molecular and cellular biology32(23)4794-810(2012 Dec)
Nadra K,M?dard JJ,Mul JD,Han GS,Gr?s S,Pende M,Metzger D,Chambon P,Cuppen E,Saulnier-Blache JS,Carman GM,Desvergne B,Chrast R

Aging of myelinating glial cells predominantly affects lipid metabolism and immune response pathways.
Glia60(5)751-60(2012 May)
Verdier V,Cs?rdi G,de Preux-Charles AS,M?dard JJ,Smit AB,Verheijen MH,Bergmann S,Chrast R

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Lpin1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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