gRNAs
The following Chd7 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Chd7 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
| CloneID | ORa28032 | |
| Clone ID Related Accession (Same CDS sequence) | XM_003749899.4 , NM_001107906.2 | |
| Accession Version | NM_001107906.2 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 8958bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector |  User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-08-13 | |
| Organism | Rattus norvegicus(Norway rat) | |
| Product | chromodomain-helicase-DNA-binding protein 7 | |
| Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AABR07047165.1, AABR07047166.1 and CK843800.1. On or before Aug 14, 2019 this sequence version replaced XM_003749899.4, NM_001107906.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END## | |
1 | ATGGCGGATC CAGGAATGAT GAGTCTTTTT GGCGAGGATG GGAATATTTT CAGCGAAGGC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_001101376.2 |
| CDS | 521..9478 |
| Translation |
Target ORF information:
Target ORF information:
|
 NM_001107906.2 |
1 | ATGGCGGATC CAGGAATGAT GAGTCTTTTT GGCGAGGATG GGAATATTTT CAGCGAAGGC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | ORa16501 | |
| Clone ID Related Accession (Same CDS sequence) | XM_008775893.2 | |
| Accession Version | XM_008775893.2 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 8985bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2016-07-25 | |
| Organism | Rattus norvegicus(Norway rat) | |
| Product | chromodomain-helicase-DNA-binding protein 7 isoform X3 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (AC_000073.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008775893.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGGCGGATC CAGGAATGAT GAGTCTTTTT GGCGAGGATG GGAATATTTT CAGCGAAGGC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_008774115.1 |
| CDS | 174..9158 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_008775893.2 |
1 | ATGGCGGATC CAGGAATGAT GAGTCTTTTT GGCGAGGATG GGAATATTTT CAGCGAAGGC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | ORa28032 | |
| Clone ID Related Accession (Same CDS sequence) | XM_003749899.4 , NM_001107906.2 | |
| Accession Version | XM_003749899.4 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 8958bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2016-07-25 | |
| Organism | Rattus norvegicus(Norway rat) | |
| Product | chromodomain-helicase-DNA-binding protein 7 isoform X1 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005104.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_003749899.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGGCGGATC CAGGAATGAT GAGTCTTTTT GGCGAGGATG GGAATATTTT CAGCGAAGGC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_003749947.1 |
| CDS | 465..9422 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_003749899.4 |
1 | ATGGCGGATC CAGGAATGAT GAGTCTTTTT GGCGAGGATG GGAATATTTT CAGCGAAGGC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | ORa28033 | |
| Clone ID Related Accession (Same CDS sequence) | XM_006237848.3 | |
| Accession Version | XM_006237848.3 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 8955bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2016-07-25 | |
| Organism | Rattus norvegicus(Norway rat) | |
| Product | chromodomain-helicase-DNA-binding protein 7 isoform X2 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005104.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006237848.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGGCGGATC CAGGAATGAT GAGTCTTTTT GGCGAGGATG GGAATATTTT CAGCGAAGGC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_006237910.1 |
| CDS | 465..9419 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_006237848.3 |
1 | ATGGCGGATC CAGGAATGAT GAGTCTTTTT GGCGAGGATG GGAATATTTT CAGCGAAGGC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
A critical role for the chromatin remodeller CHD7 in anterior mesoderm during cardiovascular development. |
CHD7 deficiency in "Looper", a new mouse model of CHARGE syndrome, results in ossicle malformation, otosclerosis and hearing impairment. |
Genetic analysis of the CHD7 gene in Korean patients with CHARGE syndrome. |
Brg1 governs distinct pathways to direct multiple aspects of mammalian neural crest cell development. |
Impact of lactobacilli on orally acquired listeriosis. |
