gRNAs
The following Ift122 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ift122 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
| CloneID | ORa47147 | |
| Clone ID Related Accession (Same CDS sequence) | NM_001009416.2 | |
| Accession Version | NM_001009416.2 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 2220bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector |  User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-10-10 | |
| Organism | Rattus norvegicus(Norway rat) | |
| Product | intraflagellar transport protein 122 homolog isoform 2 | |
| Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AABR07061824.1 and AABR07061825.1. On Jun 10, 2015 this sequence version replaced NM_001009416.1. Transcript Variant: This variant (2) lacks several exons and its 3' terminal exon extends past a splice site that is used in variant 1. This results in a novel 3' coding region and 3' UTR, compared to variant 1. It encodes isoform 2 which is shorter and has a distinct C-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC087667.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end. | |
1 | ATGAGGGCCG TGTTGACGTG GAGAGACAAA GCGGAGCAGT GTATCTACGA CCTTGCATTT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_001009416.1 |
| CDS | 85..2304 |
| Translation |
Target ORF information:
Target ORF information:
|
 NM_001009416.2 |
1 | ATGAGGGCCG TGTTGACGTG GAGAGACAAA GCGGAGCAGT GTATCTACGA CCTTGCATTT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | ORa16329 | |
| Clone ID Related Accession (Same CDS sequence) | NM_001309521.1 | |
| Accession Version | NM_001309521.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 3549bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-10-09 | |
| Organism | Rattus norvegicus(Norway rat) | |
| Product | intraflagellar transport protein 122 homolog isoform 1 | |
| Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AABR07061824.1 and AABR07061825.1. On or before Jun 9, 2015 this sequence version replaced XM_001057925.4, XM_001054604.4. Transcript Variant: This variant (1) encodes the longer isoform (1). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments and orthologous data. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end. | |
1 | ATGAGGGCCG TGTTGACGTG GAGAGACAAA GCGGAGCAGT GTATCTACGA CCTTGCATTT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_001296450.1 |
| CDS | 85..3633 |
| Translation |
Target ORF information:
Target ORF information:
|
NM_001309521.1 |
1 | ATGAGGGCCG TGTTGACGTG GAGAGACAAA GCGGAGCAGT GTATCTACGA CCTTGCATTT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | ORa16328 | |
| Clone ID Related Accession (Same CDS sequence) | XM_006237067.2 | |
| Accession Version | XM_006237067.2 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 3726bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2016-07-25 | |
| Organism | Rattus norvegicus(Norway rat) | |
| Product | intraflagellar transport protein 122 homolog isoform X1 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005103.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006237067.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGAGGGCCG TGTTGACGTG GAGAGACAAA GCGGAGCAGT GTATCTACGA CCTTGCATTT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_006237129.1 |
| CDS | 178..3903 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_006237067.2 |
1 | ATGAGGGCCG TGTTGACGTG GAGAGACAAA GCGGAGCAGT GTATCTACGA CCTTGCATTT |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
Ciliopathy-associated mutations of IFT122 impair ciliary protein trafficking but not ciliogenesis. |
Intraflagellar transport-A complex mediates ciliary entry and retrograde trafficking of ciliary G protein-coupled receptors. |
The IFT-A complex regulates Shh signaling through cilia structure and membrane protein trafficking. |
A role for the primary cilium in Notch signaling and epidermal differentiation during skin development. |
