Lrsam1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Lrsam1
Entrez Gene ID	311866
Full Name	leucine rich repeat and sterile alpha motif containing 1
Synonyms	RGD1564403
General protein information	Preferred Names leucine rich repeat and sterile alpha motif containing 1 Names E3 ubiquitin-protein ligase LRSAM1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 3 Map Location: 3p11

mRNA and Protein(s)

mRNA	Protein	Name
NM_001107833.1	NP_001101303.1	E3 ubiquitin-protein ligase LRSAM1

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Lrsam1	NP_955006.1
Rattus norvegicus (Norway rat)	Lrsam1	NP_001101303.1
Gallus gallus (chicken)	LRSAM1	XP_004945836.1
Homo sapiens (human)	LRSAM1	NP_612370.3
Canis lupus familiaris (dog)	LRSAM1	XP_548450.2
	LRSAM1	XP_002800233.1
Pan troglodytes (chimpanzee)	LRSAM1	XP_001149113.1
Bos taurus (cattle)	LRSAM1	NP_001068764.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005053	XP_001230957.2
Xenopus tropicalis (tropical clawed frog)	lrsam1	XP_002940776.2
Danio rerio (zebrafish)	lrsam1	NP_001093474.1

Gene Ontology
Bibliography

Related articles in PubMed

PHF23 (plant homeodomain finger protein 23) negatively regulates cell autophagy by promoting ubiquitination and degradation of E3 ligase LRSAM1.
Wang Z, Hu J, Li G, Qu L, He Q, Lou Y, Song Q, Ma D, Chen Y
Autophagy10(12)2158-70(2014)

The LRR and RING domain protein LRSAM1 is an E3 ligase crucial for ubiquitin-dependent autophagy of intracellular Salmonella Typhimurium.
Huett A, Heath RJ, Begun J, Sassi SO, Baxt LA, Vyas JM, Goldberg MB, Xavier RJ
Cell host & microbe12(6)778-90(2012 Dec)

Regulation of Tsg101 expression by the steadiness box: a role of Tsg101-associated ligase.
McDonald B, Martin-Serrano J
Molecular biology of the cell19(2)754-63(2008 Feb)

Tal, a Tsg101-specific E3 ubiquitin ligase, regulates receptor endocytosis and retrovirus budding.
Amit I, Yakir L, Katz M, Zwang Y, Marmor MD, Citri A, Shtiegman K, Alroy I, Tuvia S, Reiss Y, Roubini E, Cohen M, Wides R, Bacharach E, Schubert U, Yarden Y
Genes & development18(14)1737-52(2004 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Lrsam1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Lrsam1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa06097	NM_001107833.1 Latest version!	Rattus norvegicus leucine rich repeat and sterile alpha motif containing 1 (Lrsam1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Lrsam1 ( NM_001107833.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001101303.1 Rattus norvegicus leucine rich repeat and sterile alpha motif containing 1 (Lrsam1), mRNA.

Price & Availability↑

CloneID	ORa06097
Clone ID Related Accession (Same CDS sequence)	NM_001107833.1
Accession Version	NM_001107833.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2184bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-27
Organism	Rattus norvegicus(Norway rat)
Product	E3 ubiquitin-protein ligase LRSAM1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CH474001.2. On or before Oct 4, 2007 this sequence version replaced XM_231157.4, XM_001078484.1. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGCCTCTCT TCTTTCGGAA GCGGAAACCC AGTGAGGAAG CTCGGAAACG CCTGGAGTAC 
CAGATGTGTC TGGCAAAAGA AGCTGGGGCA GATGACATCC TTGACATCTC TAAGTGCGAG 
CTTTCCGAGA TTCCATTTGG GGCTTTTGCA ACGTGCAAAG TTCTACAGAA AAAGGTGTTG 
ATTGTCCACA CAAACCATCT CACCTCCCTG CTGCCCAAGT CCTGCAGCCT CTTGAGCCTC 
GCCACCATCA AGGTTCTGGA TCTCCATGAC AACCAGCTGA CAGCCCTTCC TGACGATATT 
GGGCAGCTGA CAGCCCTGCA GGTATTGAAT GTAGAAAGGA ATCAACTGAC ACACCTCCCA 
CGCTCTGTTG GGAACCTGCT GCAGCTCCAG ACCCTCAACG TAAAAGACAA CAAGCTAAAG 
GAGCTTCCGG ACACCCTGGG GGAGCTGCGG AGCCTGCGTA CCCTCGACAT CAGTGAAAAT 
GAGATCCAGA GACTTCCCCA GATGCTGGCT CATGTGCGGA CCCTGGAGAC GCTGAGCCTC 
GATGCCTTGT CAATGGTCTA CCCCCCACCA GAGGTGTGTG GCGCTGGCAC TGCGGCCGTG 
CAGCAGTTCC TCTGCAAAGA GTCAGGCCTG GACTATTACC CACCTTCTCA GTACCTGCTG 
CCAGTCCTGG AGCAAGATGG AGCCGAGAAC TCCCAGGACA GCCCTGATGG ACCCACACGC 
AGATTCTCCA GGGAGGAGGC TGAATGGCAG AATCGGTTCT CCGACTACGA GAAGCGAAAG 
GAGCAGAAGA TGCTGGAGAA GCTGGAGTTC GAGCGGCGCC TGGACCTCGG GCAGCGGGAG 
CATGCTGAGC TGCTCCAGCA GAGCCACAGC CACAAGGACG AGATCCTGCA GACGGTCAAG 
CAGGAGCAGA CACGGCTCGA GCAGGGCCTG AGTGAGCGCC AGCGCTGCCT GGATGCAGAA 
CGGCAGCAGC TGCAGGAGCA GCTCAAGCAG TCGGAGCAGA GCATTGCCAG CCGCATCCAG 
AGACTCCTGC AGGACAATCA GAGGCAAAAG AAGAGTTCTG AGATTCTGAA ATCACTGGAG 
AATGAGAGAA TACGAATGGA GCAGCTGATG TCCATTACCC AGGAGGAGAC CGAGAACCTC 
AGGCAGCGTG AGATCGCCGC CGCCATGCAG CAGATGCTGA CCGAGAGCTG TAAGAGCCGG 
CTCATCCAGA TGGCCTATGA GTCCCAGAGG CAGAGCCTGG TGCAGCAGGC CTGTTCCAGC 
ATGGCTGAAA TGGACAAGCG GTTCCAGCAG ATTCTGTCAT GGCAGCAGAT GGACCAGAAC 
AAAGCCATCA GCCAGATCCT TCAGGAGAGT GTGATGCAGA AGGCTGCATT CGAGGCTCTC 
CAGGTAAAGA AAGACCTCAC GCATCGGCAG ATCAGGAGCC AGATTAGGCT AATAGAAACT 
GAGTTACTGC AGCTGACACA GCTGGAGTTA AAGAGGAAGT CCCTGGACAC AGAGACGCTT 
CAGGAGATGG TCTCAGAGCA GCGCTGGGCG CTCAGCAACC TGCTCCAGCA GCTCCTCAAA 
GAGAAGAAGC AGCGGGAAGA GGAGCTCCAT GGCATCCTGG CGGAATTAGA GGCCAAGAGT 
GAAACGAAGC AGGAAAATTA CTGGCTCATC CAGTACCAAC GGCTTTTGAA CCAGAAGCCT 
TTGTCCTTGA AGCTGCAGGA AGAAGGCATG GAGCGGCAGC TGGTGGCCCT GCTGGTGGAG 
CTGTCTGCTG AGCACTACCT GCCCCTCTTC GCCCACCACC GCATCACACT GGACATGCTG 
AGCCGGATGG GTCCTGGAGA TCTGGCTAAG GTGGGTGTCT CAGAAGCAGG CCTGCAACAT 
GAAATCCTGC GAAGAGCCCG GGACCTGCTG GATGTGGCCA GGGTCCAACC AGAGTTGAAA 
CCACCCAAGA ATGAGGTCTT TGGTGTCTCT GAGCCCCCCA CAGCCCCTCA GGAGCTTCCT 
GAGTCCGTGA GACCATCTGC CCCGCCAGCT GAACTGGACG TGCCGACCTC AGAGTGTGTT 
GTGTGCCTGG AACGTGAGGC CCAGATGGTC TTCCTCACCT GCGGCCATGT CTGCTGCTGC 
CAGCAGTGCT GCCAGCCGCT GCGCACCTGC CCACTGTGCC GCCAGGAGAT CTCCCAGCGC 
CTCCGGATCT ACCACAGCAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001101303.1
CDS	299..2482
Translation	MPLFFRKRKPSEEARKRLEYQMCLAKEAGADDILDISKCELSEIPFGAFATCKVLQKKVLIVHTNHLTSLLPKSCSLLSLATIKVLDLHDNQLTALPDDIGQLTALQVLNVERNQLTHLPRSVGNLLQLQTLNVKDNKLKELPDTLGELRSLRTLDISENEIQRLPQMLAHVRTLETLSLDALSMVYPPPEVCGAGTAAVQQFLCKESGLDYYPPSQYLLPVLEQDGAENSQDSPDGPTRRFSREEAEWQNRFSDYEKRKEQKMLEKLEFERRLDLGQREHAELLQQSHSHKDEILQTVKQEQTRLEQGLSERQRCLDAERQQLQEQLKQSEQSIASRIQRLLQDNQRQKKSSEILKSLENERIRMEQLMSITQEETENLRQREIAAAMQQMLTESCKSRLIQMAYESQRQSLVQQACSSMAEMDKRFQQILSWQQMDQNKAISQILQESVMQKAAFEALQVKKDLTHRQIRSQIRLIETELLQLTQLELKRKSLDTETLQEMVSEQRWALSNLLQQLLKEKKQREEELHGILAELEAKSETKQENYWLIQYQRLLNQKPLSLKLQEEGMERQLVALLVELSAEHYLPLFAHHRITLDMLSRMGPGDLAKVGVSEAGLQHEILRRARDLLDVARVQPELKPPKNEVFGVSEPPTAPQELPESVRPSAPPAELDVPTSECVVCLEREAQMVFLTCGHVCCCQQCCQPLRTCPLCRQEISQRLRIYHSS

Target ORF information:

RefSeq Version	NM_001107833.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus leucine rich repeat and sterile alpha motif containing 1 (Lrsam1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001107833.1

ORF Insert Sequence:

ATGCCTCTCT TCTTTCGGAA GCGGAAACCC AGTGAGGAAG CTCGGAAACG CCTGGAGTAC 
CAGATGTGTC TGGCAAAAGA AGCTGGGGCA GATGACATCC TTGACATCTC TAAGTGCGAG 
CTTTCCGAGA TTCCATTTGG GGCTTTTGCA ACGTGCAAAG TTCTACAGAA AAAGGTGTTG 
ATTGTCCACA CAAACCATCT CACCTCCCTG CTGCCCAAGT CCTGCAGCCT CTTGAGCCTC 
GCCACCATCA AGGTTCTGGA TCTCCATGAC AACCAGCTGA CAGCCCTTCC TGACGATATT 
GGGCAGCTGA CAGCCCTGCA GGTATTGAAT GTAGAAAGGA ATCAACTGAC ACACCTCCCA 
CGCTCTGTTG GGAACCTGCT GCAGCTCCAG ACCCTCAACG TAAAAGACAA CAAGCTAAAG 
GAGCTTCCGG ACACCCTGGG GGAGCTGCGG AGCCTGCGTA CCCTCGACAT CAGTGAAAAT 
GAGATCCAGA GACTTCCCCA GATGCTGGCT CATGTGCGGA CCCTGGAGAC GCTGAGCCTC 
GATGCCTTGT CAATGGTCTA CCCCCCACCA GAGGTGTGTG GCGCTGGCAC TGCGGCCGTG 
CAGCAGTTCC TCTGCAAAGA GTCAGGCCTG GACTATTACC CACCTTCTCA GTACCTGCTG 
CCAGTCCTGG AGCAAGATGG AGCCGAGAAC TCCCAGGACA GCCCTGATGG ACCCACACGC 
AGATTCTCCA GGGAGGAGGC TGAATGGCAG AATCGGTTCT CCGACTACGA GAAGCGAAAG 
GAGCAGAAGA TGCTGGAGAA GCTGGAGTTC GAGCGGCGCC TGGACCTCGG GCAGCGGGAG 
CATGCTGAGC TGCTCCAGCA GAGCCACAGC CACAAGGACG AGATCCTGCA GACGGTCAAG 
CAGGAGCAGA CACGGCTCGA GCAGGGCCTG AGTGAGCGCC AGCGCTGCCT GGATGCAGAA 
CGGCAGCAGC TGCAGGAGCA GCTCAAGCAG TCGGAGCAGA GCATTGCCAG CCGCATCCAG 
AGACTCCTGC AGGACAATCA GAGGCAAAAG AAGAGTTCTG AGATTCTGAA ATCACTGGAG 
AATGAGAGAA TACGAATGGA GCAGCTGATG TCCATTACCC AGGAGGAGAC CGAGAACCTC 
AGGCAGCGTG AGATCGCCGC CGCCATGCAG CAGATGCTGA CCGAGAGCTG TAAGAGCCGG 
CTCATCCAGA TGGCCTATGA GTCCCAGAGG CAGAGCCTGG TGCAGCAGGC CTGTTCCAGC 
ATGGCTGAAA TGGACAAGCG GTTCCAGCAG ATTCTGTCAT GGCAGCAGAT GGACCAGAAC 
AAAGCCATCA GCCAGATCCT TCAGGAGAGT GTGATGCAGA AGGCTGCATT CGAGGCTCTC 
CAGGTAAAGA AAGACCTCAC GCATCGGCAG ATCAGGAGCC AGATTAGGCT AATAGAAACT 
GAGTTACTGC AGCTGACACA GCTGGAGTTA AAGAGGAAGT CCCTGGACAC AGAGACGCTT 
CAGGAGATGG TCTCAGAGCA GCGCTGGGCG CTCAGCAACC TGCTCCAGCA GCTCCTCAAA 
GAGAAGAAGC AGCGGGAAGA GGAGCTCCAT GGCATCCTGG CGGAATTAGA GGCCAAGAGT 
GAAACGAAGC AGGAAAATTA CTGGCTCATC CAGTACCAAC GGCTTTTGAA CCAGAAGCCT 
TTGTCCTTGA AGCTGCAGGA AGAAGGCATG GAGCGGCAGC TGGTGGCCCT GCTGGTGGAG 
CTGTCTGCTG AGCACTACCT GCCCCTCTTC GCCCACCACC GCATCACACT GGACATGCTG 
AGCCGGATGG GTCCTGGAGA TCTGGCTAAG GTGGGTGTCT CAGAAGCAGG CCTGCAACAT 
GAAATCCTGC GAAGAGCCCG GGACCTGCTG GATGTGGCCA GGGTCCAACC AGAGTTGAAA 
CCACCCAAGA ATGAGGTCTT TGGTGTCTCT GAGCCCCCCA CAGCCCCTCA GGAGCTTCCT 
GAGTCCGTGA GACCATCTGC CCCGCCAGCT GAACTGGACG TGCCGACCTC AGAGTGTGTT 
GTGTGCCTGG AACGTGAGGC CCAGATGGTC TTCCTCACCT GCGGCCATGT CTGCTGCTGC 
CAGCAGTGCT GCCAGCCGCT GCGCACCTGC CCACTGTGCC GCCAGGAGAT CTCCCAGCGC 
CTCCGGATCT ACCACAGCAG CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

PHF23 (plant homeodomain finger protein 23) negatively regulates cell autophagy by promoting ubiquitination and degradation of E3 ligase LRSAM1.
Autophagy10(12)2158-70(2014)
Wang Z,Hu J,Li G,Qu L,He Q,Lou Y,Song Q,Ma D,Chen Y

The LRR and RING domain protein LRSAM1 is an E3 ligase crucial for ubiquitin-dependent autophagy of intracellular Salmonella Typhimurium.
Cell host & microbe12(6)778-90(2012 Dec)
Huett A,Heath RJ,Begun J,Sassi SO,Baxt LA,Vyas JM,Goldberg MB,Xavier RJ

Regulation of Tsg101 expression by the steadiness box: a role of Tsg101-associated ligase.
Molecular biology of the cell19(2)754-63(2008 Feb)
McDonald B,Martin-Serrano J

Tal, a Tsg101-specific E3 ubiquitin ligase, regulates receptor endocytosis and retrovirus budding.
Genes & development18(14)1737-52(2004 Jul)
Amit I,Yakir L,Katz M,Zwang Y,Marmor MD,Citri A,Shtiegman K,Alroy I,Tuvia S,Reiss Y,Roubini E,Cohen M,Wides R,Bacharach E,Schubert U,Yarden Y

見積依頼

Eメール

japanmarket@genscript.com

電話

03-6811-6572

FAX

03-6811-6573
050-3174-7589(受注専用)

オンラインフォーム

オンライン注文

Lrsam1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

見積依頼

Eメール

電話

FAX

オンラインフォーム

サポート

会社情報

サポート

会社情報