Usp20 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Usp20
Entrez Gene ID	311856
Full Name	ubiquitin specific peptidase 20
General protein information	Preferred Names ubiquitin specific peptidase 20 Names ubiquitin carboxyl-terminal hydrolase 20 ubiquitin specific protease 20
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 3 Map Location: 3p12

mRNA and Protein(s)

mRNA	Protein	Name
XM_006233916.3	XP_006233978.1	ubiquitin carboxyl-terminal hydrolase 20 isoform X1
XM_017591848.1	XP_017447337.1	ubiquitin carboxyl-terminal hydrolase 20 isoform X3
XM_017591847.1	XP_017447336.1	ubiquitin carboxyl-terminal hydrolase 20 isoform X2
NM_001107827.3	NP_001101297.1	ubiquitin carboxyl-terminal hydrolase 20

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Usp20	NP_083122.1
	USP20	XP_001107144.1
Canis lupus familiaris (dog)	USP20	XP_537816.3
Bos taurus (cattle)	USP20	NP_001098803.1
Gallus gallus (chicken)	USP20	XP_004945924.1
Danio rerio (zebrafish)	usp20	NP_957281.2
Drosophila melanogaster (fruit fly)	CG8494	NP_610943.2
Xenopus tropicalis (tropical clawed frog)	usp20	NP_001090641.1
Homo sapiens (human)	USP20	NP_001008563.2
Pan troglodytes (chimpanzee)	USP20	XP_520313.2
Rattus norvegicus (Norway rat)	Usp20	NP_001101297.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003821	XP_310382.7

Gene Ontology
Bibliography

Related articles in PubMed

Effect of Prolonged Iodine Overdose on Type 2 Iodothyronine Deiodinase Ubiquitination-Related Enzymes in the Rat Pituitary.
Zhang X, Jiang Y, Han W, Liu A, Xie X, Han C, Fan C, Wang H, Zhang H, Ding S, Shan Z, Teng W
Biological trace element research174(2)377-386(2016 Dec)

USP33 regulates centrosome biogenesis via deubiquitination of the centriolar protein CP110.
Li J, D'Angiolella V, Seeley ES, Kim S, Kobayashi T, Fu W, Campos EI, Pagano M, Dynlacht BD
Nature495(7440)255-9(2013 Mar)

The deubiquitinases USP33 and USP20 coordinate beta2 adrenergic receptor recycling and resensitization.
Berthouze M, Venkataramanan V, Li Y, Shenoy SK
The EMBO journal28(12)1684-96(2009 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

High iodine intake had no discernible effects on USP20. Temporary increases in USP33 and USP19 mRNA levels were observed. The enzymes related to D2 ubiquitination change with prolonged high iodine intake. Increased D2 ubiquitination suppresses the activity of D2, causing a decrease in negative feedback of the hypothalamic-pituitary-thyroid axis.
Title: Effect of Prolonged Iodine Overdose on Type 2 Iodothyronine Deiodinase Ubiquitination-Related Enzymes in the Rat Pituitary.

The following Usp20 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Usp20 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa11485	NM_001107827.3 Latest version!	Rattus norvegicus ubiquitin specific peptidase 20 (Usp20), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
ORa11485	XM_006233916.3 Latest version!	Rattus norvegicus ubiquitin specific peptidase 20 (Usp20), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
ORa25696	XM_017591848.1 Latest version!	Rattus norvegicus ubiquitin specific peptidase 20 (Usp20), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
ORa47087	XM_017591847.1 Latest version!	Rattus norvegicus ubiquitin specific peptidase 20 (Usp20), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Usp20 ( NM_001107827.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001101297.1 Rattus norvegicus ubiquitin specific peptidase 20 (Usp20), mRNA.

Price & Availability↑

CloneID	ORa11485
Clone ID Related Accession (Same CDS sequence)	XM_006233916.3 , NM_001107827.3
Accession Version	NM_001107827.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2748bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-30
Organism	Rattus norvegicus(Norway rat)
Product	ubiquitin carboxyl-terminal hydrolase 20
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC125306.5. On Jul 7, 2012 this sequence version replaced NM_001107827.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGGGGACG CCAGAGACAT CTGCCCTCAC CTGGACTCCA TAGGGGAGGT GACCAAAGAG 
GACTTGCTGC TTAAATCTAA GGGGACCTGC CAGTCCTGTG GTGTTGCTGG ACCTAACCTA 
TGGGCTTGCC TCCAGGTCAC CTGCCCCTAT GTTGGCTGTG GAGAGTCTTT TGCTGACCAT 
AGCAGCATTC ATGCACAGGT GAAAAGGCAC AACCTGACTG TGAACCTGAC TACGTTACGG 
GTGTGGTGCT ACGCCTGTGA GCGGGAAGTC TTTCTGGAGC AGCGCCTGGC AGTCCACCTG 
CCCAGCTCCT CAGCCAGGCT CTCAGAGCAG GACTCCCCAC CACCCTGCCA CCCTCTGAAA 
GCTGTCCCTA TTGCCGTGGC TGATGAGGGA GAGTCTGAGT CTGAGGACGA TGACCTGAAG 
CCTCGAGGCC TCACAGGCAT GAAGAACCTG GGGAACTCCT GTTACATGAA TGCTGCCCTG 
CAGGCCTTGT CCAATTGCCC CCCACTCACC CAGTTCTTCC TGGAGTGTGG TGGCCTGGTG 
CGAACAGACA AGAAGCCAGC CTTGTGCAAA AGTTACCAGA AGTTGATCTC TGAGGTCTGG 
CACAAGAAAC GGCCAAGCTA TGTGGTCCCC ACCAGCCTAT CCCATGGGAT CAAGTTGGTC 
AACCCGATGT TCCGAGGCTA TGCCCAGCAG GACACCCAGG AGTTCCTACG CTGCCTGATG 
GACCAGCTTC ACGAGGAGCT CAAGGAGCCC ATGGTAGCTG CTGTGGCAGC TCTCACTGAT 
GCTCGGGACT CAGATTCCAG TGACACAGAT GAGAGGCGTG ATGGCGACCG GAGCCCTTCA 
GAGGACGAGT TTCTGTCCTG TGACTCGAGC AGTGACAGGG GTGAGGGTGA TGGGCAGGGC 
CGTGGTGGAG GCAGCTCAAA GGCTGAGATG GAGCTGCTGA TCTCAGACGA GGCGGGCCGA 
GCCATCTCTG AGAAAGAGCG AATGAAGGAC CGCAAGTTCT CCTGGGGCCA GCAGCGCACC 
AACTCGGAGC AAGTGGATGA GGACGCTGAT GTGGACACAG CCATGGCCTC CCTTGACGAA 
CAATCTAGAG AGGCCCAGCC ACCGTCACCA AGGCCTACCA GCCCCTGCCA GACCCCAGAG 
CCAGACAATG AAGTCCACAT TCGCAGCTCC TCTCGCCCCT GCAGCCCTGT CCACCACCAC 
GAGGGCCATA CCAAGCTGTC CAGCAGCCCT CCTCGTGCAA GCCCCGTGAG GATGGGGCCA 
TCGTACGTGC TCAAGAAAGC CCAGGTGCCG AGTACTGGTG GCCGGAGGCG GAAGGAACAG 
AGCTACCGCA GTGTCATCTC AGATGTCTTC AATGGCTCTG TCCTCAGCCT GGTGCAGTGT 
CTTACCTGTG ATCGGGTGTC TACCACAGTG GAGACATTCC AGGACCTGTC ATTGCCCATC 
CCTGGCAAGG AAGACCTAGC CAAACTTCAC TCTGCCATCT ACCAGAATGT ACCAGCCAAG 
CCGGGTGCCT GTGGGGACAG CTATTCCTCC CAGGGCTGGC TGGCCTTCAT CGTGGAGTAC 
ATCCGACGGT TTGTGGTATC CTGTACCCCC AGCTGGTTTT GGGGACCTGT TGTCACACTG 
GAAGACTGCC TGGCTGCTTT CTTTGCTGCT GATGAGCTGA AGGGTGACAA TATGTACAGC 
TGTGAGCGCT GTAAGAAGCT TCGGAATGGC GTGAAGTACT GTAAAGTCCT CTGTCTGCCT 
GAGATTCTGT GTGTCCACCT GAAGCGCTTC CGGCATGAGG TGATGTACTC CTTCAAAGTC 
AGCAGCCATG TCTCTTTCCC CCTGGAGGGA CTTGACCTAC GCCCTTTTCT AGCCAAGGAG 
TGCACATCCC AGGTCACCAC CTATGACCTC CTCTCTGTCA TTTGTCACCA CGGCACAGCA 
GGCAGTGGGC ACTACATTGC CTACTGTCAG AACGTGATCA ATGGGCAGTG GTATGAGTTT 
GATGACCAGT ACGTCACCGA GGTCCATGAG ACTGTGGTAC AGAATGTGGA GGCCTACGTG 
CTCTTCTACA GGAAGAGCAG TGAGGAGGCC ATGCGTGAGC GGCAGCAGGT GGTGTCCCTG 
GCTGCCATGC AGGAACCCAG CCTGCTCCGC TTCTACGTGT CCCGAGAATG GCTCAACAAG 
TTCAACACCT TTGCAGAGCC AGGGCCCATC ACCAACCACA CCTTCCTGTG CCCCCATGGA 
GGTATCCCAC CCAATAAATA CCATTACATT GATGACCTGG TGGTCATTCT GCCGCAGAGC 
GTCTGGGAGC ACCTGTACAG CAGGTTTGGG GGCGGCCCTG CTGTGAACCA TCTGTATGTG 
TGCTCCATCT GCCAGGTGGA GATTGAGGCC CTGGCCAAGC GCAGGAGGGT GGAGATTGAC 
ACTTTCATCA AGCTGAACAA GGCATTCCAA GCCGAGGAGT CTCCCGGGGT CATCTACTGC 
ATCAGCATGC ACTGGTTCCG AGAGTGGGAG GCTTTCGTCA AGGGGAAGGA CAGCGAGCCT 
CCAGGGCCCA TCGACAACAG CAGGATTGCA CAGGTCAAAG GCAGTGGTCA TATCCAGCTA 
AAGCAGGGGG CTGACTACGG GCAGATTTCT GAGGAGACCT GGACCTATCT GAACAGTCTG 
TATGGGGGTG GCCCTGAGAT TGCCATCCGG CAGAGTGTGG CCCAGCTCCC AGACCCAGAA 
AGCTTGCATG GAGAGCAGAA GATTGAGGCT GAAACCCGGG CTCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001101297.1
CDS	179..2926
Translation	MGDARDICPHLDSIGEVTKEDLLLKSKGTCQSCGVAGPNLWACLQVTCPYVGCGESFADHSSIHAQVKRHNLTVNLTTLRVWCYACEREVFLEQRLAVHLPSSSARLSEQDSPPPCHPLKAVPIAVADEGESESEDDDLKPRGLTGMKNLGNSCYMNAALQALSNCPPLTQFFLECGGLVRTDKKPALCKSYQKLISEVWHKKRPSYVVPTSLSHGIKLVNPMFRGYAQQDTQEFLRCLMDQLHEELKEPMVAAVAALTDARDSDSSDTDERRDGDRSPSEDEFLSCDSSSDRGEGDGQGRGGGSSKAEMELLISDEAGRAISEKERMKDRKFSWGQQRTNSEQVDEDADVDTAMASLDEQSREAQPPSPRPTSPCQTPEPDNEVHIRSSSRPCSPVHHHEGHTKLSSSPPRASPVRMGPSYVLKKAQVPSTGGRRRKEQSYRSVISDVFNGSVLSLVQCLTCDRVSTTVETFQDLSLPIPGKEDLAKLHSAIYQNVPAKPGACGDSYSSQGWLAFIVEYIRRFVVSCTPSWFWGPVVTLEDCLAAFFAADELKGDNMYSCERCKKLRNGVKYCKVLCLPEILCVHLKRFRHEVMYSFKVSSHVSFPLEGLDLRPFLAKECTSQVTTYDLLSVICHHGTAGSGHYIAYCQNVINGQWYEFDDQYVTEVHETVVQNVEAYVLFYRKSSEEAMRERQQVVSLAAMQEPSLLRFYVSREWLNKFNTFAEPGPITNHTFLCPHGGIPPNKYHYIDDLVVILPQSVWEHLYSRFGGGPAVNHLYVCSICQVEIEALAKRRRVEIDTFIKLNKAFQAEESPGVIYCISMHWFREWEAFVKGKDSEPPGPIDNSRIAQVKGSGHIQLKQGADYGQISEETWTYLNSLYGGGPEIAIRQSVAQLPDPESLHGEQKIEAETRAL

Target ORF information:

RefSeq Version	NM_001107827.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ubiquitin specific peptidase 20 (Usp20), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001107827.3

ORF Insert Sequence:

ATGGGGGACG CCAGAGACAT CTGCCCTCAC CTGGACTCCA TAGGGGAGGT GACCAAAGAG 
GACTTGCTGC TTAAATCTAA GGGGACCTGC CAGTCCTGTG GTGTTGCTGG ACCTAACCTA 
TGGGCTTGCC TCCAGGTCAC CTGCCCCTAT GTTGGCTGTG GAGAGTCTTT TGCTGACCAT 
AGCAGCATTC ATGCACAGGT GAAAAGGCAC AACCTGACTG TGAACCTGAC TACGTTACGG 
GTGTGGTGCT ACGCCTGTGA GCGGGAAGTC TTTCTGGAGC AGCGCCTGGC AGTCCACCTG 
CCCAGCTCCT CAGCCAGGCT CTCAGAGCAG GACTCCCCAC CACCCTGCCA CCCTCTGAAA 
GCTGTCCCTA TTGCCGTGGC TGATGAGGGA GAGTCTGAGT CTGAGGACGA TGACCTGAAG 
CCTCGAGGCC TCACAGGCAT GAAGAACCTG GGGAACTCCT GTTACATGAA TGCTGCCCTG 
CAGGCCTTGT CCAATTGCCC CCCACTCACC CAGTTCTTCC TGGAGTGTGG TGGCCTGGTG 
CGAACAGACA AGAAGCCAGC CTTGTGCAAA AGTTACCAGA AGTTGATCTC TGAGGTCTGG 
CACAAGAAAC GGCCAAGCTA TGTGGTCCCC ACCAGCCTAT CCCATGGGAT CAAGTTGGTC 
AACCCGATGT TCCGAGGCTA TGCCCAGCAG GACACCCAGG AGTTCCTACG CTGCCTGATG 
GACCAGCTTC ACGAGGAGCT CAAGGAGCCC ATGGTAGCTG CTGTGGCAGC TCTCACTGAT 
GCTCGGGACT CAGATTCCAG TGACACAGAT GAGAGGCGTG ATGGCGACCG GAGCCCTTCA 
GAGGACGAGT TTCTGTCCTG TGACTCGAGC AGTGACAGGG GTGAGGGTGA TGGGCAGGGC 
CGTGGTGGAG GCAGCTCAAA GGCTGAGATG GAGCTGCTGA TCTCAGACGA GGCGGGCCGA 
GCCATCTCTG AGAAAGAGCG AATGAAGGAC CGCAAGTTCT CCTGGGGCCA GCAGCGCACC 
AACTCGGAGC AAGTGGATGA GGACGCTGAT GTGGACACAG CCATGGCCTC CCTTGACGAA 
CAATCTAGAG AGGCCCAGCC ACCGTCACCA AGGCCTACCA GCCCCTGCCA GACCCCAGAG 
CCAGACAATG AAGTCCACAT TCGCAGCTCC TCTCGCCCCT GCAGCCCTGT CCACCACCAC 
GAGGGCCATA CCAAGCTGTC CAGCAGCCCT CCTCGTGCAA GCCCCGTGAG GATGGGGCCA 
TCGTACGTGC TCAAGAAAGC CCAGGTGCCG AGTACTGGTG GCCGGAGGCG GAAGGAACAG 
AGCTACCGCA GTGTCATCTC AGATGTCTTC AATGGCTCTG TCCTCAGCCT GGTGCAGTGT 
CTTACCTGTG ATCGGGTGTC TACCACAGTG GAGACATTCC AGGACCTGTC ATTGCCCATC 
CCTGGCAAGG AAGACCTAGC CAAACTTCAC TCTGCCATCT ACCAGAATGT ACCAGCCAAG 
CCGGGTGCCT GTGGGGACAG CTATTCCTCC CAGGGCTGGC TGGCCTTCAT CGTGGAGTAC 
ATCCGACGGT TTGTGGTATC CTGTACCCCC AGCTGGTTTT GGGGACCTGT TGTCACACTG 
GAAGACTGCC TGGCTGCTTT CTTTGCTGCT GATGAGCTGA AGGGTGACAA TATGTACAGC 
TGTGAGCGCT GTAAGAAGCT TCGGAATGGC GTGAAGTACT GTAAAGTCCT CTGTCTGCCT 
GAGATTCTGT GTGTCCACCT GAAGCGCTTC CGGCATGAGG TGATGTACTC CTTCAAAGTC 
AGCAGCCATG TCTCTTTCCC CCTGGAGGGA CTTGACCTAC GCCCTTTTCT AGCCAAGGAG 
TGCACATCCC AGGTCACCAC CTATGACCTC CTCTCTGTCA TTTGTCACCA CGGCACAGCA 
GGCAGTGGGC ACTACATTGC CTACTGTCAG AACGTGATCA ATGGGCAGTG GTATGAGTTT 
GATGACCAGT ACGTCACCGA GGTCCATGAG ACTGTGGTAC AGAATGTGGA GGCCTACGTG 
CTCTTCTACA GGAAGAGCAG TGAGGAGGCC ATGCGTGAGC GGCAGCAGGT GGTGTCCCTG 
GCTGCCATGC AGGAACCCAG CCTGCTCCGC TTCTACGTGT CCCGAGAATG GCTCAACAAG 
TTCAACACCT TTGCAGAGCC AGGGCCCATC ACCAACCACA CCTTCCTGTG CCCCCATGGA 
GGTATCCCAC CCAATAAATA CCATTACATT GATGACCTGG TGGTCATTCT GCCGCAGAGC 
GTCTGGGAGC ACCTGTACAG CAGGTTTGGG GGCGGCCCTG CTGTGAACCA TCTGTATGTG 
TGCTCCATCT GCCAGGTGGA GATTGAGGCC CTGGCCAAGC GCAGGAGGGT GGAGATTGAC 
ACTTTCATCA AGCTGAACAA GGCATTCCAA GCCGAGGAGT CTCCCGGGGT CATCTACTGC 
ATCAGCATGC ACTGGTTCCG AGAGTGGGAG GCTTTCGTCA AGGGGAAGGA CAGCGAGCCT 
CCAGGGCCCA TCGACAACAG CAGGATTGCA CAGGTCAAAG GCAGTGGTCA TATCCAGCTA 
AAGCAGGGGG CTGACTACGG GCAGATTTCT GAGGAGACCT GGACCTATCT GAACAGTCTG 
TATGGGGGTG GCCCTGAGAT TGCCATCCGG CAGAGTGTGG CCCAGCTCCC AGACCCAGAA 
AGCTTGCATG GAGAGCAGAA GATTGAGGCT GAAACCCGGG CTCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Usp20 ( XM_006233916.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006233978.1 Rattus norvegicus ubiquitin specific peptidase 20 (Usp20), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa11485
Clone ID Related Accession (Same CDS sequence)	XM_006233916.3 , NM_001107827.3
Accession Version	XM_006233916.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2748bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	ubiquitin carboxyl-terminal hydrolase 20 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005102.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006233916.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGACG CCAGAGACAT CTGCCCTCAC CTGGACTCCA TAGGGGAGGT GACCAAAGAG 
GACTTGCTGC TTAAATCTAA GGGGACCTGC CAGTCCTGTG GTGTTGCTGG ACCTAACCTA 
TGGGCTTGCC TCCAGGTCAC CTGCCCCTAT GTTGGCTGTG GAGAGTCTTT TGCTGACCAT 
AGCAGCATTC ATGCACAGGT GAAAAGGCAC AACCTGACTG TGAACCTGAC TACGTTACGG 
GTGTGGTGCT ACGCCTGTGA GCGGGAAGTC TTTCTGGAGC AGCGCCTGGC AGTCCACCTG 
CCCAGCTCCT CAGCCAGGCT CTCAGAGCAG GACTCCCCAC CACCCTGCCA CCCTCTGAAA 
GCTGTCCCTA TTGCCGTGGC TGATGAGGGA GAGTCTGAGT CTGAGGACGA TGACCTGAAG 
CCTCGAGGCC TCACAGGCAT GAAGAACCTG GGGAACTCCT GTTACATGAA TGCTGCCCTG 
CAGGCCTTGT CCAATTGCCC CCCACTCACC CAGTTCTTCC TGGAGTGTGG TGGCCTGGTG 
CGAACAGACA AGAAGCCAGC CTTGTGCAAA AGTTACCAGA AGTTGATCTC TGAGGTCTGG 
CACAAGAAAC GGCCAAGCTA TGTGGTCCCC ACCAGCCTAT CCCATGGGAT CAAGTTGGTC 
AACCCGATGT TCCGAGGCTA TGCCCAGCAG GACACCCAGG AGTTCCTACG CTGCCTGATG 
GACCAGCTTC ACGAGGAGCT CAAGGAGCCC ATGGTAGCTG CTGTGGCAGC TCTCACTGAT 
GCTCGGGACT CAGATTCCAG TGACACAGAT GAGAGGCGTG ATGGCGACCG GAGCCCTTCA 
GAGGACGAGT TTCTGTCCTG TGACTCGAGC AGTGACAGGG GTGAGGGTGA TGGGCAGGGC 
CGTGGTGGAG GCAGCTCAAA GGCTGAGATG GAGCTGCTGA TCTCAGACGA GGCGGGCCGA 
GCCATCTCTG AGAAAGAGCG AATGAAGGAC CGCAAGTTCT CCTGGGGCCA GCAGCGCACC 
AACTCGGAGC AAGTGGATGA GGACGCTGAT GTGGACACAG CCATGGCCTC CCTTGACGAA 
CAATCTAGAG AGGCCCAGCC ACCGTCACCA AGGCCTACCA GCCCCTGCCA GACCCCAGAG 
CCAGACAATG AAGTCCACAT TCGCAGCTCC TCTCGCCCCT GCAGCCCTGT CCACCACCAC 
GAGGGCCATA CCAAGCTGTC CAGCAGCCCT CCTCGTGCAA GCCCCGTGAG GATGGGGCCA 
TCGTACGTGC TCAAGAAAGC CCAGGTGCCG AGTACTGGTG GCCGGAGGCG GAAGGAACAG 
AGCTACCGCA GTGTCATCTC AGATGTCTTC AATGGCTCTG TCCTCAGCCT GGTGCAGTGT 
CTTACCTGTG ATCGGGTGTC TACCACAGTG GAGACATTCC AGGACCTGTC ATTGCCCATC 
CCTGGCAAGG AAGACCTAGC CAAACTTCAC TCTGCCATCT ACCAGAATGT ACCAGCCAAG 
CCGGGTGCCT GTGGGGACAG CTATTCCTCC CAGGGCTGGC TGGCCTTCAT CGTGGAGTAC 
ATCCGACGGT TTGTGGTATC CTGTACCCCC AGCTGGTTTT GGGGACCTGT TGTCACACTG 
GAAGACTGCC TGGCTGCTTT CTTTGCTGCT GATGAGCTGA AGGGTGACAA TATGTACAGC 
TGTGAGCGCT GTAAGAAGCT TCGGAATGGC GTGAAGTACT GTAAAGTCCT CTGTCTGCCT 
GAGATTCTGT GTGTCCACCT GAAGCGCTTC CGGCATGAGG TGATGTACTC CTTCAAAGTC 
AGCAGCCATG TCTCTTTCCC CCTGGAGGGA CTTGACCTAC GCCCTTTTCT AGCCAAGGAG 
TGCACATCCC AGGTCACCAC CTATGACCTC CTCTCTGTCA TTTGTCACCA CGGCACAGCA 
GGCAGTGGGC ACTACATTGC CTACTGTCAG AACGTGATCA ATGGGCAGTG GTATGAGTTT 
GATGACCAGT ACGTCACCGA GGTCCATGAG ACTGTGGTAC AGAATGTGGA GGCCTACGTG 
CTCTTCTACA GGAAGAGCAG TGAGGAGGCC ATGCGTGAGC GGCAGCAGGT GGTGTCCCTG 
GCTGCCATGC AGGAACCCAG CCTGCTCCGC TTCTACGTGT CCCGAGAATG GCTCAACAAG 
TTCAACACCT TTGCAGAGCC AGGGCCCATC ACCAACCACA CCTTCCTGTG CCCCCATGGA 
GGTATCCCAC CCAATAAATA CCATTACATT GATGACCTGG TGGTCATTCT GCCGCAGAGC 
GTCTGGGAGC ACCTGTACAG CAGGTTTGGG GGCGGCCCTG CTGTGAACCA TCTGTATGTG 
TGCTCCATCT GCCAGGTGGA GATTGAGGCC CTGGCCAAGC GCAGGAGGGT GGAGATTGAC 
ACTTTCATCA AGCTGAACAA GGCATTCCAA GCCGAGGAGT CTCCCGGGGT CATCTACTGC 
ATCAGCATGC ACTGGTTCCG AGAGTGGGAG GCTTTCGTCA AGGGGAAGGA CAGCGAGCCT 
CCAGGGCCCA TCGACAACAG CAGGATTGCA CAGGTCAAAG GCAGTGGTCA TATCCAGCTA 
AAGCAGGGGG CTGACTACGG GCAGATTTCT GAGGAGACCT GGACCTATCT GAACAGTCTG 
TATGGGGGTG GCCCTGAGAT TGCCATCCGG CAGAGTGTGG CCCAGCTCCC AGACCCAGAA 
AGCTTGCATG GAGAGCAGAA GATTGAGGCT GAAACCCGGG CTCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006233978.1
CDS	1311..4058
Translation	MGDARDICPHLDSIGEVTKEDLLLKSKGTCQSCGVAGPNLWACLQVTCPYVGCGESFADHSSIHAQVKRHNLTVNLTTLRVWCYACEREVFLEQRLAVHLPSSSARLSEQDSPPPCHPLKAVPIAVADEGESESEDDDLKPRGLTGMKNLGNSCYMNAALQALSNCPPLTQFFLECGGLVRTDKKPALCKSYQKLISEVWHKKRPSYVVPTSLSHGIKLVNPMFRGYAQQDTQEFLRCLMDQLHEELKEPMVAAVAALTDARDSDSSDTDERRDGDRSPSEDEFLSCDSSSDRGEGDGQGRGGGSSKAEMELLISDEAGRAISEKERMKDRKFSWGQQRTNSEQVDEDADVDTAMASLDEQSREAQPPSPRPTSPCQTPEPDNEVHIRSSSRPCSPVHHHEGHTKLSSSPPRASPVRMGPSYVLKKAQVPSTGGRRRKEQSYRSVISDVFNGSVLSLVQCLTCDRVSTTVETFQDLSLPIPGKEDLAKLHSAIYQNVPAKPGACGDSYSSQGWLAFIVEYIRRFVVSCTPSWFWGPVVTLEDCLAAFFAADELKGDNMYSCERCKKLRNGVKYCKVLCLPEILCVHLKRFRHEVMYSFKVSSHVSFPLEGLDLRPFLAKECTSQVTTYDLLSVICHHGTAGSGHYIAYCQNVINGQWYEFDDQYVTEVHETVVQNVEAYVLFYRKSSEEAMRERQQVVSLAAMQEPSLLRFYVSREWLNKFNTFAEPGPITNHTFLCPHGGIPPNKYHYIDDLVVILPQSVWEHLYSRFGGGPAVNHLYVCSICQVEIEALAKRRRVEIDTFIKLNKAFQAEESPGVIYCISMHWFREWEAFVKGKDSEPPGPIDNSRIAQVKGSGHIQLKQGADYGQISEETWTYLNSLYGGGPEIAIRQSVAQLPDPESLHGEQKIEAETRAL

Target ORF information:

RefSeq Version	XM_006233916.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ubiquitin specific peptidase 20 (Usp20), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006233916.3

ORF Insert Sequence:

ATGGGGGACG CCAGAGACAT CTGCCCTCAC CTGGACTCCA TAGGGGAGGT GACCAAAGAG 
GACTTGCTGC TTAAATCTAA GGGGACCTGC CAGTCCTGTG GTGTTGCTGG ACCTAACCTA 
TGGGCTTGCC TCCAGGTCAC CTGCCCCTAT GTTGGCTGTG GAGAGTCTTT TGCTGACCAT 
AGCAGCATTC ATGCACAGGT GAAAAGGCAC AACCTGACTG TGAACCTGAC TACGTTACGG 
GTGTGGTGCT ACGCCTGTGA GCGGGAAGTC TTTCTGGAGC AGCGCCTGGC AGTCCACCTG 
CCCAGCTCCT CAGCCAGGCT CTCAGAGCAG GACTCCCCAC CACCCTGCCA CCCTCTGAAA 
GCTGTCCCTA TTGCCGTGGC TGATGAGGGA GAGTCTGAGT CTGAGGACGA TGACCTGAAG 
CCTCGAGGCC TCACAGGCAT GAAGAACCTG GGGAACTCCT GTTACATGAA TGCTGCCCTG 
CAGGCCTTGT CCAATTGCCC CCCACTCACC CAGTTCTTCC TGGAGTGTGG TGGCCTGGTG 
CGAACAGACA AGAAGCCAGC CTTGTGCAAA AGTTACCAGA AGTTGATCTC TGAGGTCTGG 
CACAAGAAAC GGCCAAGCTA TGTGGTCCCC ACCAGCCTAT CCCATGGGAT CAAGTTGGTC 
AACCCGATGT TCCGAGGCTA TGCCCAGCAG GACACCCAGG AGTTCCTACG CTGCCTGATG 
GACCAGCTTC ACGAGGAGCT CAAGGAGCCC ATGGTAGCTG CTGTGGCAGC TCTCACTGAT 
GCTCGGGACT CAGATTCCAG TGACACAGAT GAGAGGCGTG ATGGCGACCG GAGCCCTTCA 
GAGGACGAGT TTCTGTCCTG TGACTCGAGC AGTGACAGGG GTGAGGGTGA TGGGCAGGGC 
CGTGGTGGAG GCAGCTCAAA GGCTGAGATG GAGCTGCTGA TCTCAGACGA GGCGGGCCGA 
GCCATCTCTG AGAAAGAGCG AATGAAGGAC CGCAAGTTCT CCTGGGGCCA GCAGCGCACC 
AACTCGGAGC AAGTGGATGA GGACGCTGAT GTGGACACAG CCATGGCCTC CCTTGACGAA 
CAATCTAGAG AGGCCCAGCC ACCGTCACCA AGGCCTACCA GCCCCTGCCA GACCCCAGAG 
CCAGACAATG AAGTCCACAT TCGCAGCTCC TCTCGCCCCT GCAGCCCTGT CCACCACCAC 
GAGGGCCATA CCAAGCTGTC CAGCAGCCCT CCTCGTGCAA GCCCCGTGAG GATGGGGCCA 
TCGTACGTGC TCAAGAAAGC CCAGGTGCCG AGTACTGGTG GCCGGAGGCG GAAGGAACAG 
AGCTACCGCA GTGTCATCTC AGATGTCTTC AATGGCTCTG TCCTCAGCCT GGTGCAGTGT 
CTTACCTGTG ATCGGGTGTC TACCACAGTG GAGACATTCC AGGACCTGTC ATTGCCCATC 
CCTGGCAAGG AAGACCTAGC CAAACTTCAC TCTGCCATCT ACCAGAATGT ACCAGCCAAG 
CCGGGTGCCT GTGGGGACAG CTATTCCTCC CAGGGCTGGC TGGCCTTCAT CGTGGAGTAC 
ATCCGACGGT TTGTGGTATC CTGTACCCCC AGCTGGTTTT GGGGACCTGT TGTCACACTG 
GAAGACTGCC TGGCTGCTTT CTTTGCTGCT GATGAGCTGA AGGGTGACAA TATGTACAGC 
TGTGAGCGCT GTAAGAAGCT TCGGAATGGC GTGAAGTACT GTAAAGTCCT CTGTCTGCCT 
GAGATTCTGT GTGTCCACCT GAAGCGCTTC CGGCATGAGG TGATGTACTC CTTCAAAGTC 
AGCAGCCATG TCTCTTTCCC CCTGGAGGGA CTTGACCTAC GCCCTTTTCT AGCCAAGGAG 
TGCACATCCC AGGTCACCAC CTATGACCTC CTCTCTGTCA TTTGTCACCA CGGCACAGCA 
GGCAGTGGGC ACTACATTGC CTACTGTCAG AACGTGATCA ATGGGCAGTG GTATGAGTTT 
GATGACCAGT ACGTCACCGA GGTCCATGAG ACTGTGGTAC AGAATGTGGA GGCCTACGTG 
CTCTTCTACA GGAAGAGCAG TGAGGAGGCC ATGCGTGAGC GGCAGCAGGT GGTGTCCCTG 
GCTGCCATGC AGGAACCCAG CCTGCTCCGC TTCTACGTGT CCCGAGAATG GCTCAACAAG 
TTCAACACCT TTGCAGAGCC AGGGCCCATC ACCAACCACA CCTTCCTGTG CCCCCATGGA 
GGTATCCCAC CCAATAAATA CCATTACATT GATGACCTGG TGGTCATTCT GCCGCAGAGC 
GTCTGGGAGC ACCTGTACAG CAGGTTTGGG GGCGGCCCTG CTGTGAACCA TCTGTATGTG 
TGCTCCATCT GCCAGGTGGA GATTGAGGCC CTGGCCAAGC GCAGGAGGGT GGAGATTGAC 
ACTTTCATCA AGCTGAACAA GGCATTCCAA GCCGAGGAGT CTCCCGGGGT CATCTACTGC 
ATCAGCATGC ACTGGTTCCG AGAGTGGGAG GCTTTCGTCA AGGGGAAGGA CAGCGAGCCT 
CCAGGGCCCA TCGACAACAG CAGGATTGCA CAGGTCAAAG GCAGTGGTCA TATCCAGCTA 
AAGCAGGGGG CTGACTACGG GCAGATTTCT GAGGAGACCT GGACCTATCT GAACAGTCTG 
TATGGGGGTG GCCCTGAGAT TGCCATCCGG CAGAGTGTGG CCCAGCTCCC AGACCCAGAA 
AGCTTGCATG GAGAGCAGAA GATTGAGGCT GAAACCCGGG CTCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Usp20 ( XM_017591848.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017447337.1 Rattus norvegicus ubiquitin specific peptidase 20 (Usp20), transcript variant X3, mRNA.

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CloneID	ORa25696
Clone ID Related Accession (Same CDS sequence)	XM_017591848.1
Accession Version	XM_017591848.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2310bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	ubiquitin carboxyl-terminal hydrolase 20 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005102.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAACC TGGGGAACTC CTGTTACATG AATGCTGCCC TGCAGGCCTT GTCCAATTGC 
CCCCCACTCA CCCAGTTCTT CCTGGAGTGT GGTGGCCTGG TGCGAACAGA CAAGAAGCCA 
GCCTTGTGCA AAAGTTACCA GAAGTTGATC TCTGAGGTCT GGCACAAGAA ACGGCCAAGC 
TATGTGGTCC CCACCAGCCT ATCCCATGGG ATCAAGTTGG TCAACCCGAT GTTCCGAGGC 
TATGCCCAGC AGGACACCCA GGAGTTCCTA CGCTGCCTGA TGGACCAGCT TCACGAGGAG 
CTCAAGGAGC CCATGGTAGC TGCTGTGGCA GCTCTCACTG ATGCTCGGGA CTCAGATTCC 
AGTGACACAG ATGAGAGGCG TGATGGCGAC CGGAGCCCTT CAGAGGACGA GTTTCTGTCC 
TGTGACTCGA GCAGTGACAG GGGTGAGGGT GATGGGCAGG GCCGTGGTGG AGGCAGCTCA 
AAGGCTGAGA TGGAGCTGCT GATCTCAGAC GAGGCGGGCC GAGCCATCTC TGAGAAAGAG 
CGAATGAAGG ACCGCAAGTT CTCCTGGGGC CAGCAGCGCA CCAACTCGGA GCAAGTGGAT 
GAGGACGCTG ATGTGGACAC AGCCATGGCC TCCCTTGACG AACAATCTAG AGAGGCCCAG 
CCACCGTCAC CAAGGCCTAC CAGCCCCTGC CAGACCCCAG AGCCAGACAA TGAAGTCCAC 
ATTCGCAGCT CCTCTCGCCC CTGCAGCCCT GTCCACCACC ACGAGGGCCA TACCAAGCTG 
TCCAGCAGCC CTCCTCGTGC AAGCCCCGTG AGGATGGGGC CATCGTACGT GCTCAAGAAA 
GCCCAGGTGC CGAGTACTGG TGGCCGGAGG CGGAAGGAAC AGAGCTACCG CAGTGTCATC 
TCAGATGTCT TCAATGGCTC TGTCCTCAGC CTGGTGCAGT GTCTTACCTG TGATCGGGTG 
TCTACCACAG TGGAGACATT CCAGGACCTG TCATTGCCCA TCCCTGGCAA GGAAGACCTA 
GCCAAACTTC ACTCTGCCAT CTACCAGAAT GTACCAGCCA AGCCGGGTGC CTGTGGGGAC 
AGCTATTCCT CCCAGGGCTG GCTGGCCTTC ATCGTGGAGT ACATCCGACG GTTTGTGGTA 
TCCTGTACCC CCAGCTGGTT TTGGGGACCT GTTGTCACAC TGGAAGACTG CCTGGCTGCT 
TTCTTTGCTG CTGATGAGCT GAAGGGTGAC AATATGTACA GCTGTGAGCG CTGTAAGAAG 
CTTCGGAATG GCGTGAAGTA CTGTAAAGTC CTCTGTCTGC CTGAGATTCT GTGTGTCCAC 
CTGAAGCGCT TCCGGCATGA GGTGATGTAC TCCTTCAAAG TCAGCAGCCA TGTCTCTTTC 
CCCCTGGAGG GACTTGACCT ACGCCCTTTT CTAGCCAAGG AGTGCACATC CCAGGTCACC 
ACCTATGACC TCCTCTCTGT CATTTGTCAC CACGGCACAG CAGGCAGTGG GCACTACATT 
GCCTACTGTC AGAACGTGAT CAATGGGCAG TGGTATGAGT TTGATGACCA GTACGTCACC 
GAGGTCCATG AGACTGTGGT ACAGAATGTG GAGGCCTACG TGCTCTTCTA CAGGAAGAGC 
AGTGAGGAGG CCATGCGTGA GCGGCAGCAG GTGGTGTCCC TGGCTGCCAT GCAGGAACCC 
AGCCTGCTCC GCTTCTACGT GTCCCGAGAA TGGCTCAACA AGTTCAACAC CTTTGCAGAG 
CCAGGGCCCA TCACCAACCA CACCTTCCTG TGCCCCCATG GAGGTATCCC ACCCAATAAA 
TACCATTACA TTGATGACCT GGTGGTCATT CTGCCGCAGA GCGTCTGGGA GCACCTGTAC 
AGCAGGTTTG GGGGCGGCCC TGCTGTGAAC CATCTGTATG TGTGCTCCAT CTGCCAGGTG 
GAGATTGAGG CCCTGGCCAA GCGCAGGAGG GTGGAGATTG ACACTTTCAT CAAGCTGAAC 
AAGGCATTCC AAGCCGAGGA GTCTCCCGGG GTCATCTACT GCATCAGCAT GCACTGGTTC 
CGAGAGTGGG AGGCTTTCGT CAAGGGGAAG GACAGCGAGC CTCCAGGGCC CATCGACAAC 
AGCAGGATTG CACAGGTCAA AGGCAGTGGT CATATCCAGC TAAAGCAGGG GGCTGACTAC 
GGGCAGATTT CTGAGGAGAC CTGGACCTAT CTGAACAGTC TGTATGGGGG TGGCCCTGAG 
ATTGCCATCC GGCAGAGTGT GGCCCAGCTC CCAGACCCAG AAAGCTTGCA TGGAGAGCAG 
AAGATTGAGG CTGAAACCCG GGCTCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017447337.1
CDS	1653..3962
Translation	MKNLGNSCYMNAALQALSNCPPLTQFFLECGGLVRTDKKPALCKSYQKLISEVWHKKRPSYVVPTSLSHGIKLVNPMFRGYAQQDTQEFLRCLMDQLHEELKEPMVAAVAALTDARDSDSSDTDERRDGDRSPSEDEFLSCDSSSDRGEGDGQGRGGGSSKAEMELLISDEAGRAISEKERMKDRKFSWGQQRTNSEQVDEDADVDTAMASLDEQSREAQPPSPRPTSPCQTPEPDNEVHIRSSSRPCSPVHHHEGHTKLSSSPPRASPVRMGPSYVLKKAQVPSTGGRRRKEQSYRSVISDVFNGSVLSLVQCLTCDRVSTTVETFQDLSLPIPGKEDLAKLHSAIYQNVPAKPGACGDSYSSQGWLAFIVEYIRRFVVSCTPSWFWGPVVTLEDCLAAFFAADELKGDNMYSCERCKKLRNGVKYCKVLCLPEILCVHLKRFRHEVMYSFKVSSHVSFPLEGLDLRPFLAKECTSQVTTYDLLSVICHHGTAGSGHYIAYCQNVINGQWYEFDDQYVTEVHETVVQNVEAYVLFYRKSSEEAMRERQQVVSLAAMQEPSLLRFYVSREWLNKFNTFAEPGPITNHTFLCPHGGIPPNKYHYIDDLVVILPQSVWEHLYSRFGGGPAVNHLYVCSICQVEIEALAKRRRVEIDTFIKLNKAFQAEESPGVIYCISMHWFREWEAFVKGKDSEPPGPIDNSRIAQVKGSGHIQLKQGADYGQISEETWTYLNSLYGGGPEIAIRQSVAQLPDPESLHGEQKIEAETRAL

Target ORF information:

RefSeq Version	XM_017591848.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ubiquitin specific peptidase 20 (Usp20), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017591848.1

ORF Insert Sequence:

ATGAAGAACC TGGGGAACTC CTGTTACATG AATGCTGCCC TGCAGGCCTT GTCCAATTGC 
CCCCCACTCA CCCAGTTCTT CCTGGAGTGT GGTGGCCTGG TGCGAACAGA CAAGAAGCCA 
GCCTTGTGCA AAAGTTACCA GAAGTTGATC TCTGAGGTCT GGCACAAGAA ACGGCCAAGC 
TATGTGGTCC CCACCAGCCT ATCCCATGGG ATCAAGTTGG TCAACCCGAT GTTCCGAGGC 
TATGCCCAGC AGGACACCCA GGAGTTCCTA CGCTGCCTGA TGGACCAGCT TCACGAGGAG 
CTCAAGGAGC CCATGGTAGC TGCTGTGGCA GCTCTCACTG ATGCTCGGGA CTCAGATTCC 
AGTGACACAG ATGAGAGGCG TGATGGCGAC CGGAGCCCTT CAGAGGACGA GTTTCTGTCC 
TGTGACTCGA GCAGTGACAG GGGTGAGGGT GATGGGCAGG GCCGTGGTGG AGGCAGCTCA 
AAGGCTGAGA TGGAGCTGCT GATCTCAGAC GAGGCGGGCC GAGCCATCTC TGAGAAAGAG 
CGAATGAAGG ACCGCAAGTT CTCCTGGGGC CAGCAGCGCA CCAACTCGGA GCAAGTGGAT 
GAGGACGCTG ATGTGGACAC AGCCATGGCC TCCCTTGACG AACAATCTAG AGAGGCCCAG 
CCACCGTCAC CAAGGCCTAC CAGCCCCTGC CAGACCCCAG AGCCAGACAA TGAAGTCCAC 
ATTCGCAGCT CCTCTCGCCC CTGCAGCCCT GTCCACCACC ACGAGGGCCA TACCAAGCTG 
TCCAGCAGCC CTCCTCGTGC AAGCCCCGTG AGGATGGGGC CATCGTACGT GCTCAAGAAA 
GCCCAGGTGC CGAGTACTGG TGGCCGGAGG CGGAAGGAAC AGAGCTACCG CAGTGTCATC 
TCAGATGTCT TCAATGGCTC TGTCCTCAGC CTGGTGCAGT GTCTTACCTG TGATCGGGTG 
TCTACCACAG TGGAGACATT CCAGGACCTG TCATTGCCCA TCCCTGGCAA GGAAGACCTA 
GCCAAACTTC ACTCTGCCAT CTACCAGAAT GTACCAGCCA AGCCGGGTGC CTGTGGGGAC 
AGCTATTCCT CCCAGGGCTG GCTGGCCTTC ATCGTGGAGT ACATCCGACG GTTTGTGGTA 
TCCTGTACCC CCAGCTGGTT TTGGGGACCT GTTGTCACAC TGGAAGACTG CCTGGCTGCT 
TTCTTTGCTG CTGATGAGCT GAAGGGTGAC AATATGTACA GCTGTGAGCG CTGTAAGAAG 
CTTCGGAATG GCGTGAAGTA CTGTAAAGTC CTCTGTCTGC CTGAGATTCT GTGTGTCCAC 
CTGAAGCGCT TCCGGCATGA GGTGATGTAC TCCTTCAAAG TCAGCAGCCA TGTCTCTTTC 
CCCCTGGAGG GACTTGACCT ACGCCCTTTT CTAGCCAAGG AGTGCACATC CCAGGTCACC 
ACCTATGACC TCCTCTCTGT CATTTGTCAC CACGGCACAG CAGGCAGTGG GCACTACATT 
GCCTACTGTC AGAACGTGAT CAATGGGCAG TGGTATGAGT TTGATGACCA GTACGTCACC 
GAGGTCCATG AGACTGTGGT ACAGAATGTG GAGGCCTACG TGCTCTTCTA CAGGAAGAGC 
AGTGAGGAGG CCATGCGTGA GCGGCAGCAG GTGGTGTCCC TGGCTGCCAT GCAGGAACCC 
AGCCTGCTCC GCTTCTACGT GTCCCGAGAA TGGCTCAACA AGTTCAACAC CTTTGCAGAG 
CCAGGGCCCA TCACCAACCA CACCTTCCTG TGCCCCCATG GAGGTATCCC ACCCAATAAA 
TACCATTACA TTGATGACCT GGTGGTCATT CTGCCGCAGA GCGTCTGGGA GCACCTGTAC 
AGCAGGTTTG GGGGCGGCCC TGCTGTGAAC CATCTGTATG TGTGCTCCAT CTGCCAGGTG 
GAGATTGAGG CCCTGGCCAA GCGCAGGAGG GTGGAGATTG ACACTTTCAT CAAGCTGAAC 
AAGGCATTCC AAGCCGAGGA GTCTCCCGGG GTCATCTACT GCATCAGCAT GCACTGGTTC 
CGAGAGTGGG AGGCTTTCGT CAAGGGGAAG GACAGCGAGC CTCCAGGGCC CATCGACAAC 
AGCAGGATTG CACAGGTCAA AGGCAGTGGT CATATCCAGC TAAAGCAGGG GGCTGACTAC 
GGGCAGATTT CTGAGGAGAC CTGGACCTAT CTGAACAGTC TGTATGGGGG TGGCCCTGAG 
ATTGCCATCC GGCAGAGTGT GGCCCAGCTC CCAGACCCAG AAAGCTTGCA TGGAGAGCAG 
AAGATTGAGG CTGAAACCCG GGCTCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Usp20 ( XM_017591847.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017447336.1 Rattus norvegicus ubiquitin specific peptidase 20 (Usp20), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa47087
Clone ID Related Accession (Same CDS sequence)	XM_017591847.1
Accession Version	XM_017591847.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2694bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	ubiquitin carboxyl-terminal hydrolase 20 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005102.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGACG CCAGAGACAT CTGCCCTCAC CTGGACTCCA TAGGGGAGGT GACCAAAGAG 
GACTTGCTGC TTAAATCTAA GGTCACCTGC CCCTATGTTG GCTGTGGAGA GTCTTTTGCT 
GACCATAGCA GCATTCATGC ACAGGTGAAA AGGCACAACC TGACTGTGAA CCTGACTACG 
TTACGGGTGT GGTGCTACGC CTGTGAGCGG GAAGTCTTTC TGGAGCAGCG CCTGGCAGTC 
CACCTGCCCA GCTCCTCAGC CAGGCTCTCA GAGCAGGACT CCCCACCACC CTGCCACCCT 
CTGAAAGCTG TCCCTATTGC CGTGGCTGAT GAGGGAGAGT CTGAGTCTGA GGACGATGAC 
CTGAAGCCTC GAGGCCTCAC AGGCATGAAG AACCTGGGGA ACTCCTGTTA CATGAATGCT 
GCCCTGCAGG CCTTGTCCAA TTGCCCCCCA CTCACCCAGT TCTTCCTGGA GTGTGGTGGC 
CTGGTGCGAA CAGACAAGAA GCCAGCCTTG TGCAAAAGTT ACCAGAAGTT GATCTCTGAG 
GTCTGGCACA AGAAACGGCC AAGCTATGTG GTCCCCACCA GCCTATCCCA TGGGATCAAG 
TTGGTCAACC CGATGTTCCG AGGCTATGCC CAGCAGGACA CCCAGGAGTT CCTACGCTGC 
CTGATGGACC AGCTTCACGA GGAGCTCAAG GAGCCCATGG TAGCTGCTGT GGCAGCTCTC 
ACTGATGCTC GGGACTCAGA TTCCAGTGAC ACAGATGAGA GGCGTGATGG CGACCGGAGC 
CCTTCAGAGG ACGAGTTTCT GTCCTGTGAC TCGAGCAGTG ACAGGGGTGA GGGTGATGGG 
CAGGGCCGTG GTGGAGGCAG CTCAAAGGCT GAGATGGAGC TGCTGATCTC AGACGAGGCG 
GGCCGAGCCA TCTCTGAGAA AGAGCGAATG AAGGACCGCA AGTTCTCCTG GGGCCAGCAG 
CGCACCAACT CGGAGCAAGT GGATGAGGAC GCTGATGTGG ACACAGCCAT GGCCTCCCTT 
GACGAACAAT CTAGAGAGGC CCAGCCACCG TCACCAAGGC CTACCAGCCC CTGCCAGACC 
CCAGAGCCAG ACAATGAAGT CCACATTCGC AGCTCCTCTC GCCCCTGCAG CCCTGTCCAC 
CACCACGAGG GCCATACCAA GCTGTCCAGC AGCCCTCCTC GTGCAAGCCC CGTGAGGATG 
GGGCCATCGT ACGTGCTCAA GAAAGCCCAG GTGCCGAGTA CTGGTGGCCG GAGGCGGAAG 
GAACAGAGCT ACCGCAGTGT CATCTCAGAT GTCTTCAATG GCTCTGTCCT CAGCCTGGTG 
CAGTGTCTTA CCTGTGATCG GGTGTCTACC ACAGTGGAGA CATTCCAGGA CCTGTCATTG 
CCCATCCCTG GCAAGGAAGA CCTAGCCAAA CTTCACTCTG CCATCTACCA GAATGTACCA 
GCCAAGCCGG GTGCCTGTGG GGACAGCTAT TCCTCCCAGG GCTGGCTGGC CTTCATCGTG 
GAGTACATCC GACGGTTTGT GGTATCCTGT ACCCCCAGCT GGTTTTGGGG ACCTGTTGTC 
ACACTGGAAG ACTGCCTGGC TGCTTTCTTT GCTGCTGATG AGCTGAAGGG TGACAATATG 
TACAGCTGTG AGCGCTGTAA GAAGCTTCGG AATGGCGTGA AGTACTGTAA AGTCCTCTGT 
CTGCCTGAGA TTCTGTGTGT CCACCTGAAG CGCTTCCGGC ATGAGGTGAT GTACTCCTTC 
AAAGTCAGCA GCCATGTCTC TTTCCCCCTG GAGGGACTTG ACCTACGCCC TTTTCTAGCC 
AAGGAGTGCA CATCCCAGGT CACCACCTAT GACCTCCTCT CTGTCATTTG TCACCACGGC 
ACAGCAGGCA GTGGGCACTA CATTGCCTAC TGTCAGAACG TGATCAATGG GCAGTGGTAT 
GAGTTTGATG ACCAGTACGT CACCGAGGTC CATGAGACTG TGGTACAGAA TGTGGAGGCC 
TACGTGCTCT TCTACAGGAA GAGCAGTGAG GAGGCCATGC GTGAGCGGCA GCAGGTGGTG 
TCCCTGGCTG CCATGCAGGA ACCCAGCCTG CTCCGCTTCT ACGTGTCCCG AGAATGGCTC 
AACAAGTTCA ACACCTTTGC AGAGCCAGGG CCCATCACCA ACCACACCTT CCTGTGCCCC 
CATGGAGGTA TCCCACCCAA TAAATACCAT TACATTGATG ACCTGGTGGT CATTCTGCCG 
CAGAGCGTCT GGGAGCACCT GTACAGCAGG TTTGGGGGCG GCCCTGCTGT GAACCATCTG 
TATGTGTGCT CCATCTGCCA GGTGGAGATT GAGGCCCTGG CCAAGCGCAG GAGGGTGGAG 
ATTGACACTT TCATCAAGCT GAACAAGGCA TTCCAAGCCG AGGAGTCTCC CGGGGTCATC 
TACTGCATCA GCATGCACTG GTTCCGAGAG TGGGAGGCTT TCGTCAAGGG GAAGGACAGC 
GAGCCTCCAG GGCCCATCGA CAACAGCAGG ATTGCACAGG TCAAAGGCAG TGGTCATATC 
CAGCTAAAGC AGGGGGCTGA CTACGGGCAG ATTTCTGAGG AGACCTGGAC CTATCTGAAC 
AGTCTGTATG GGGGTGGCCC TGAGATTGCC ATCCGGCAGA GTGTGGCCCA GCTCCCAGAC 
CCAGAAAGCT TGCATGGAGA GCAGAAGATT GAGGCTGAAA CCCGGGCTCT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017447336.1
CDS	1311..4004
Translation	MGDARDICPHLDSIGEVTKEDLLLKSKVTCPYVGCGESFADHSSIHAQVKRHNLTVNLTTLRVWCYACEREVFLEQRLAVHLPSSSARLSEQDSPPPCHPLKAVPIAVADEGESESEDDDLKPRGLTGMKNLGNSCYMNAALQALSNCPPLTQFFLECGGLVRTDKKPALCKSYQKLISEVWHKKRPSYVVPTSLSHGIKLVNPMFRGYAQQDTQEFLRCLMDQLHEELKEPMVAAVAALTDARDSDSSDTDERRDGDRSPSEDEFLSCDSSSDRGEGDGQGRGGGSSKAEMELLISDEAGRAISEKERMKDRKFSWGQQRTNSEQVDEDADVDTAMASLDEQSREAQPPSPRPTSPCQTPEPDNEVHIRSSSRPCSPVHHHEGHTKLSSSPPRASPVRMGPSYVLKKAQVPSTGGRRRKEQSYRSVISDVFNGSVLSLVQCLTCDRVSTTVETFQDLSLPIPGKEDLAKLHSAIYQNVPAKPGACGDSYSSQGWLAFIVEYIRRFVVSCTPSWFWGPVVTLEDCLAAFFAADELKGDNMYSCERCKKLRNGVKYCKVLCLPEILCVHLKRFRHEVMYSFKVSSHVSFPLEGLDLRPFLAKECTSQVTTYDLLSVICHHGTAGSGHYIAYCQNVINGQWYEFDDQYVTEVHETVVQNVEAYVLFYRKSSEEAMRERQQVVSLAAMQEPSLLRFYVSREWLNKFNTFAEPGPITNHTFLCPHGGIPPNKYHYIDDLVVILPQSVWEHLYSRFGGGPAVNHLYVCSICQVEIEALAKRRRVEIDTFIKLNKAFQAEESPGVIYCISMHWFREWEAFVKGKDSEPPGPIDNSRIAQVKGSGHIQLKQGADYGQISEETWTYLNSLYGGGPEIAIRQSVAQLPDPESLHGEQKIEAETRAL

Target ORF information:

RefSeq Version	XM_017591847.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus ubiquitin specific peptidase 20 (Usp20), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017591847.1

ORF Insert Sequence:

ATGGGGGACG CCAGAGACAT CTGCCCTCAC CTGGACTCCA TAGGGGAGGT GACCAAAGAG 
GACTTGCTGC TTAAATCTAA GGTCACCTGC CCCTATGTTG GCTGTGGAGA GTCTTTTGCT 
GACCATAGCA GCATTCATGC ACAGGTGAAA AGGCACAACC TGACTGTGAA CCTGACTACG 
TTACGGGTGT GGTGCTACGC CTGTGAGCGG GAAGTCTTTC TGGAGCAGCG CCTGGCAGTC 
CACCTGCCCA GCTCCTCAGC CAGGCTCTCA GAGCAGGACT CCCCACCACC CTGCCACCCT 
CTGAAAGCTG TCCCTATTGC CGTGGCTGAT GAGGGAGAGT CTGAGTCTGA GGACGATGAC 
CTGAAGCCTC GAGGCCTCAC AGGCATGAAG AACCTGGGGA ACTCCTGTTA CATGAATGCT 
GCCCTGCAGG CCTTGTCCAA TTGCCCCCCA CTCACCCAGT TCTTCCTGGA GTGTGGTGGC 
CTGGTGCGAA CAGACAAGAA GCCAGCCTTG TGCAAAAGTT ACCAGAAGTT GATCTCTGAG 
GTCTGGCACA AGAAACGGCC AAGCTATGTG GTCCCCACCA GCCTATCCCA TGGGATCAAG 
TTGGTCAACC CGATGTTCCG AGGCTATGCC CAGCAGGACA CCCAGGAGTT CCTACGCTGC 
CTGATGGACC AGCTTCACGA GGAGCTCAAG GAGCCCATGG TAGCTGCTGT GGCAGCTCTC 
ACTGATGCTC GGGACTCAGA TTCCAGTGAC ACAGATGAGA GGCGTGATGG CGACCGGAGC 
CCTTCAGAGG ACGAGTTTCT GTCCTGTGAC TCGAGCAGTG ACAGGGGTGA GGGTGATGGG 
CAGGGCCGTG GTGGAGGCAG CTCAAAGGCT GAGATGGAGC TGCTGATCTC AGACGAGGCG 
GGCCGAGCCA TCTCTGAGAA AGAGCGAATG AAGGACCGCA AGTTCTCCTG GGGCCAGCAG 
CGCACCAACT CGGAGCAAGT GGATGAGGAC GCTGATGTGG ACACAGCCAT GGCCTCCCTT 
GACGAACAAT CTAGAGAGGC CCAGCCACCG TCACCAAGGC CTACCAGCCC CTGCCAGACC 
CCAGAGCCAG ACAATGAAGT CCACATTCGC AGCTCCTCTC GCCCCTGCAG CCCTGTCCAC 
CACCACGAGG GCCATACCAA GCTGTCCAGC AGCCCTCCTC GTGCAAGCCC CGTGAGGATG 
GGGCCATCGT ACGTGCTCAA GAAAGCCCAG GTGCCGAGTA CTGGTGGCCG GAGGCGGAAG 
GAACAGAGCT ACCGCAGTGT CATCTCAGAT GTCTTCAATG GCTCTGTCCT CAGCCTGGTG 
CAGTGTCTTA CCTGTGATCG GGTGTCTACC ACAGTGGAGA CATTCCAGGA CCTGTCATTG 
CCCATCCCTG GCAAGGAAGA CCTAGCCAAA CTTCACTCTG CCATCTACCA GAATGTACCA 
GCCAAGCCGG GTGCCTGTGG GGACAGCTAT TCCTCCCAGG GCTGGCTGGC CTTCATCGTG 
GAGTACATCC GACGGTTTGT GGTATCCTGT ACCCCCAGCT GGTTTTGGGG ACCTGTTGTC 
ACACTGGAAG ACTGCCTGGC TGCTTTCTTT GCTGCTGATG AGCTGAAGGG TGACAATATG 
TACAGCTGTG AGCGCTGTAA GAAGCTTCGG AATGGCGTGA AGTACTGTAA AGTCCTCTGT 
CTGCCTGAGA TTCTGTGTGT CCACCTGAAG CGCTTCCGGC ATGAGGTGAT GTACTCCTTC 
AAAGTCAGCA GCCATGTCTC TTTCCCCCTG GAGGGACTTG ACCTACGCCC TTTTCTAGCC 
AAGGAGTGCA CATCCCAGGT CACCACCTAT GACCTCCTCT CTGTCATTTG TCACCACGGC 
ACAGCAGGCA GTGGGCACTA CATTGCCTAC TGTCAGAACG TGATCAATGG GCAGTGGTAT 
GAGTTTGATG ACCAGTACGT CACCGAGGTC CATGAGACTG TGGTACAGAA TGTGGAGGCC 
TACGTGCTCT TCTACAGGAA GAGCAGTGAG GAGGCCATGC GTGAGCGGCA GCAGGTGGTG 
TCCCTGGCTG CCATGCAGGA ACCCAGCCTG CTCCGCTTCT ACGTGTCCCG AGAATGGCTC 
AACAAGTTCA ACACCTTTGC AGAGCCAGGG CCCATCACCA ACCACACCTT CCTGTGCCCC 
CATGGAGGTA TCCCACCCAA TAAATACCAT TACATTGATG ACCTGGTGGT CATTCTGCCG 
CAGAGCGTCT GGGAGCACCT GTACAGCAGG TTTGGGGGCG GCCCTGCTGT GAACCATCTG 
TATGTGTGCT CCATCTGCCA GGTGGAGATT GAGGCCCTGG CCAAGCGCAG GAGGGTGGAG 
ATTGACACTT TCATCAAGCT GAACAAGGCA TTCCAAGCCG AGGAGTCTCC CGGGGTCATC 
TACTGCATCA GCATGCACTG GTTCCGAGAG TGGGAGGCTT TCGTCAAGGG GAAGGACAGC 
GAGCCTCCAG GGCCCATCGA CAACAGCAGG ATTGCACAGG TCAAAGGCAG TGGTCATATC 
CAGCTAAAGC AGGGGGCTGA CTACGGGCAG ATTTCTGAGG AGACCTGGAC CTATCTGAAC 
AGTCTGTATG GGGGTGGCCC TGAGATTGCC ATCCGGCAGA GTGTGGCCCA GCTCCCAGAC 
CCAGAAAGCT TGCATGGAGA GCAGAAGATT GAGGCTGAAA CCCGGGCTCT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Effect of Prolonged Iodine Overdose on Type 2 Iodothyronine Deiodinase Ubiquitination-Related Enzymes in the Rat Pituitary.
Biological trace element research174(2)377-386(2016 Dec)
Zhang X,Jiang Y,Han W,Liu A,Xie X,Han C,Fan C,Wang H,Zhang H,Ding S,Shan Z,Teng W

USP33 regulates centrosome biogenesis via deubiquitination of the centriolar protein CP110.
Nature495(7440)255-9(2013 Mar)
Li J,D'Angiolella V,Seeley ES,Kim S,Kobayashi T,Fu W,Campos EI,Pagano M,Dynlacht BD

The deubiquitinases USP33 and USP20 coordinate beta2 adrenergic receptor recycling and resensitization.
The EMBO journal28(12)1684-96(2009 Jun)
Berthouze M,Venkataramanan V,Li Y,Shenoy SK

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Usp20 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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