Crat cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Crat
Entrez Gene ID	311849
Full Name	carnitine O-acetyltransferase
General protein information	Preferred Names carnitine O-acetyltransferase Names carnitine O-acetyltransferase CAT carnitine acetylase carnitine acetyltransferase
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 3 Map Location: 3p12
Summary	catalyzes the reversible transfer of an acyl group from acyl-CoA thioesters to carnitine; key enzyme in mitochondrial and endoplasmic reticulum metabolic pathways [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_017591845.1	XP_017447334.1	carnitine O-acetyltransferase isoform X2
NM_001004085.2	NP_001004085.1	carnitine O-acetyltransferase
XM_008761663.2	XP_008759885.1	carnitine O-acetyltransferase isoform X1
XM_006233823.2	XP_006233885.1	carnitine O-acetyltransferase isoform X1
XM_008761664.1	XP_008759886.1	carnitine O-acetyltransferase isoform X2

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	CRAT	XP_520305.3
	CRAT	XP_001109211.1
Rattus norvegicus (Norway rat)	Crat	NP_001004085.1
Danio rerio (zebrafish)	crata	NP_001005587.1
Drosophila melanogaster (fruit fly)	CG5122	NP_609601.1
Drosophila melanogaster (fruit fly)	CG1041	NP_649650.2
Magnaporthe oryzae (rice blast fungus)	MGG_01721	XP_003714718.1
Bos taurus (cattle)	CRAT	NP_001069055.1
Mus musculus (house mouse)	Crat	NP_031786.2
Caenorhabditis elegans (roundworm)	B0395.3	NP_510624.1
Eremothecium gossypii	AGOS_AAR124C	NP_982666.1
Homo sapiens (human)	CRAT	NP_000746.2
Canis lupus familiaris (dog)	CRAT	XP_548425.2
Gallus gallus (chicken)	CRAT	XP_004945922.1
Saccharomyces cerevisiae (baker's yeast)	CAT2	NP_013670.1
Drosophila melanogaster (fruit fly)	CG5265	NP_650605.1
Kluyveromyces lactis	KLLA0A02123g	XP_451094.1
Neurospora crassa	NCU08002	XP_962672.1
Xenopus tropicalis (tropical clawed frog)	crat.1	NP_001027489.1

Gene Ontology
Bibliography

Related articles in PubMed

Obesity and lipid stress inhibit carnitine acetyltransferase activity.
Seiler SE, Martin OJ, Noland RC, Slentz DH, DeBalsi KL, Ilkayeva OR, An J, Newgard CB, Koves TR, Muoio DM
Journal of lipid research55(4)635-44(2014 Apr)

Chromatographic separation of reaction products from the choline acetyltransferase and carnitine acetyltransferase assay: differential ChAT and CrAT activity in brain extracts from Alzheimer's disease versus controls.
Bailey JA, Lahiri DK
Journal of neurochemistry122(4)672-80(2012 Aug)

Phosphoproteome analysis of functional mitochondria isolated from resting human muscle reveals extensive phosphorylation of inner membrane protein complexes and enzymes.
Zhao X, Le?n IR, Bak S, Mogensen M, Wrzesinski K, H?jlund K, Jensen ON
Molecular & cellular proteomics : MCP10(1)M110.000299(2011 Jan)

A proteome-wide perspective on peroxisome targeting signal 1(PTS1)-Pex5p affinities.
Ghosh D, Berg JM
Journal of the American Chemical Society132(11)3973-9(2010 Mar)

The carnitine acetyltransferase gene (CRAT): a characterization of porcine transcripts with insights into the 5'-end variants of mammalian transcripts and their possible sub-cellular localization.
Robic A, Faraut T, Liaubet L, Milan D
Cellular & molecular biology letters14(1)90-9(2009)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Lipid-induced antagonism of CrAT might contribute to decreased PDH activity and glucose disposal in the context of obesity and diabetes.
Title: Obesity and lipid stress inhibit carnitine acetyltransferase activity.

this assay can be used to measure carnitine acetyltransferase activity in the same samples, and assays comparing choline O-acetyltransferase and CrAT show that carnitine acetyltransferase is highly active in neuronal tissues and in neuronal cell cultures
Title: Chromatographic separation of reaction products from the choline acetyltransferase and carnitine acetyltransferase assay: differential ChAT and CrAT activity in brain extracts from Alzheimer's disease versus controls.

The predicted full length cDNA sequence of the porcine CRAT gene was characterised and a new 5' variant for dog, rat and mouse was proposed.
Title: The carnitine acetyltransferase gene (CRAT): a characterization of porcine transcripts with insights into the 5'-end variants of mammalian transcripts and their possible sub-cellular localization.

by performing two amino acid replacements a carnitine acetyltransferase can be converted into a pseudo carnitine palmitoyltransferase in terms of substrate specificity
Title: Mutagenesis of specific amino acids converts carnitine acetyltransferase into carnitine palmitoyltransferase.

show for the first time that a single amino acid is able to determine the substrate specificity of carnitine acetyltransferase (CrAT)
Title: Redesign of carnitine acetyltransferase specificity by protein engineering.

The following Crat gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Crat cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa12444	NM_001004085.2 Latest version!	Rattus norvegicus carnitine O-acetyltransferase (Crat), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$370.30 $529.00
ORa25607	XM_008761664.1 Latest version!	Rattus norvegicus carnitine O-acetyltransferase (Crat), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00
ORa25607	XM_017591845.1 Latest version!	Rattus norvegicus carnitine O-acetyltransferase (Crat), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00
ORa25606	XM_008761663.2 Latest version!	Rattus norvegicus carnitine O-acetyltransferase (Crat), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
ORa25606	XM_006233823.2 Latest version!	Rattus norvegicus carnitine O-acetyltransferase (Crat), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Crat ( NM_001004085.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001004085.1 Rattus norvegicus carnitine O-acetyltransferase (Crat), mRNA.

Price & Availability↑

CloneID	ORa12444
Clone ID Related Accession (Same CDS sequence)	NM_001004085.2
Accession Version	NM_001004085.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1881bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Rattus norvegicus(Norway rat)
Product	carnitine O-acetyltransferase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC083616.1. On Oct 10, 2004 this sequence version replaced NM_001004085.1. ##Evidence-Data-START## Transcript exon combination :: BC083616.1, AJ620886.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END##

ATGTTAGCTT TTGCTGCCAG AACCGTGGTG AAACCCCTGG GCCTCCTCAA GCCCTCCTCC 
TTGATGAAGG TGTCTGGTCG ATTTAAGGCC CACCAGGATG CACTGCCACG GCTGCCTGTG 
CCTCCACTTC AGCAATCCCT GGACCATTAC CTCAAGGCAC TGCAGCCCAT CGTGAGTGAG 
GAAGAATGGG CTCACACCAA GCAACTGGTG GACGAGTTTC AGACCTCAGG GGGTGTAGGG 
GAACGCCTGC AGAAGGGATT GGAGCGCAGG GCCAAGAAAA TGGAGAACTG GCTGTCCGAG 
TGGTGGCTCA AGACAGCCTA TCTCCAGTTC CGCCAGCCTG TGGTCATCTA CTCCAGTCCA 
GGAGTGCTGC TGCCCAAGCA GGACTTCATG GATCTACAGG GTCAGCTCCG GTTTGCTGCC 
AAACTCATCG AGGGTGTATT GGATTTCAAG TCCATGATTG ACAACGAGAC GCTGCCAGTT 
GAGTTCCTGG GAGGACAGCC ACTGTGCATG AACCAGTATT ATCAGATCCT GTCTTCTTGC 
CGTGTGCCTG GCCTCAAGCA GGACTCAGTG GTGAACTTCT TAAAGTCAAA GAAACCACCC 
ACACATATAA CCGTGGTGCA TAACTACCAG TTCTTCGAGC TGGATGTGTA CCACAGTGAT 
GGGACACCAC TCACCTCAGA TCAGATCTTT GTGCAGCTTG AGAAAATCTG GAACTCCTCA 
CTGCAATCCA ACAAGGAGCC TGTTGGCATC CTAACCTCCA ACCACCGGAA CAGCTGGGCC 
AAGGCCTACA GCAGCCTCAT TAAAGACAAA GTAAACCGGG AGTCGGTGAA CTCCATCCAG 
AAGAGCATCT TCACTGTGTG CCTGGACAAG CAAGTGCCCA GAGTCTCAGA TGATGTCTAC 
CGCAGCCACG TAGCCGGCCA GATGCTTCAT GGTGGTGGCA GCAAGTTCAA CAGTGGCAAC 
CGCTGGTTCG ACAAGACACT GCAGTTTATT GTGGCAGAAG ATGGCTCCTG TGGGATGGTT 
TATGAACATG CAGCTGCAGA AGGGCCCCCC ATTGTCGCTC TTGTGGACCA TGTCATGGAG 
TATACAAAGA AGCCTGAACT TGTGCGGTCC CCTATGGTAC CCCTGCCTAT GCCCAAGAAG 
CTGCGGTTCA ACATCACACC TGAGATCAAG AATGACATAG AGAAGGCCAA ACAGAACATC 
AGCATCATGA TCCAGGACCT GGACATCATG ATGCTGGTTT TCCATCACTT TGGAAAGGAC 
TTCCCCAAGT CCCAGAAGCT AAGCCCTGAT GCCTTCATCC AGATAGCCCT GCAGCTGGCA 
TACTACAGGA TCTATGGGCA GGCGTGTGCC ACGTATGAAA GCGCCTCTCT GCGCATGTTT 
CACCTGGGCC GCACTGACAC CATCCGCTCC GCCTCCACAG ACTCGCTGGC CTTTGTCAAA 
GGCATGGATG ACCCCAAAGT GCCGGAGCAG CAGAGGGTGG AGCTGCTTCG AAAGGCTGTG 
CAGGCCCACC GAGCCTACAC CGACCGCGCC ATCCGAGGGG AGGCCTTTGA CCGGCACTTG 
CTGGGTCTGA AGCTACAGGC CATCGAGGAC CTGGTGAGCA TGCCTGACAT CTTCATGGAT 
ACCTCCTACG CCATTGCCAT GCACTTCAAC CTTTCTACCA GCCAGGTCCC TGCCAAGACA 
GACTGTGTCA TGTCCTTCGG ACCTGTGGTC CCAGACGGTT ACGGCATCTG CTACAATCCC 
ATGGAGGCCC ATATCAACTT TTCCGTGTCA GCCTACAACA GCTGTGCTGA GACCAACGCT 
GCCCGCATGG CTCACTACTT GGAGAAAGCT CTGCTGGACA TGCGCACCTT ACTCCAGAAC 
CACCCCAGGG CCAAGCTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001004085.1
CDS	223..2103
Translation	MLAFAARTVVKPLGLLKPSSLMKVSGRFKAHQDALPRLPVPPLQQSLDHYLKALQPIVSEEEWAHTKQLVDEFQTSGGVGERLQKGLERRAKKMENWLSEWWLKTAYLQFRQPVVIYSSPGVLLPKQDFMDLQGQLRFAAKLIEGVLDFKSMIDNETLPVEFLGGQPLCMNQYYQILSSCRVPGLKQDSVVNFLKSKKPPTHITVVHNYQFFELDVYHSDGTPLTSDQIFVQLEKIWNSSLQSNKEPVGILTSNHRNSWAKAYSSLIKDKVNRESVNSIQKSIFTVCLDKQVPRVSDDVYRSHVAGQMLHGGGSKFNSGNRWFDKTLQFIVAEDGSCGMVYEHAAAEGPPIVALVDHVMEYTKKPELVRSPMVPLPMPKKLRFNITPEIKNDIEKAKQNISIMIQDLDIMMLVFHHFGKDFPKSQKLSPDAFIQIALQLAYYRIYGQACATYESASLRMFHLGRTDTIRSASTDSLAFVKGMDDPKVPEQQRVELLRKAVQAHRAYTDRAIRGEAFDRHLLGLKLQAIEDLVSMPDIFMDTSYAIAMHFNLSTSQVPAKTDCVMSFGPVVPDGYGICYNPMEAHINFSVSAYNSCAETNAARMAHYLEKALLDMRTLLQNHPRAKL

Target ORF information:

RefSeq Version	NM_001004085.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus carnitine O-acetyltransferase (Crat), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001004085.2

ORF Insert Sequence:

ATGTTAGCTT TTGCTGCCAG AACCGTGGTG AAACCCCTGG GCCTCCTCAA GCCCTCCTCC 
TTGATGAAGG TGTCTGGTCG ATTTAAGGCC CACCAGGATG CACTGCCACG GCTGCCTGTG 
CCTCCACTTC AGCAATCCCT GGACCATTAC CTCAAGGCAC TGCAGCCCAT CGTGAGTGAG 
GAAGAATGGG CTCACACCAA GCAACTGGTG GACGAGTTTC AGACCTCAGG GGGTGTAGGG 
GAACGCCTGC AGAAGGGATT GGAGCGCAGG GCCAAGAAAA TGGAGAACTG GCTGTCCGAG 
TGGTGGCTCA AGACAGCCTA TCTCCAGTTC CGCCAGCCTG TGGTCATCTA CTCCAGTCCA 
GGAGTGCTGC TGCCCAAGCA GGACTTCATG GATCTACAGG GTCAGCTCCG GTTTGCTGCC 
AAACTCATCG AGGGTGTATT GGATTTCAAG TCCATGATTG ACAACGAGAC GCTGCCAGTT 
GAGTTCCTGG GAGGACAGCC ACTGTGCATG AACCAGTATT ATCAGATCCT GTCTTCTTGC 
CGTGTGCCTG GCCTCAAGCA GGACTCAGTG GTGAACTTCT TAAAGTCAAA GAAACCACCC 
ACACATATAA CCGTGGTGCA TAACTACCAG TTCTTCGAGC TGGATGTGTA CCACAGTGAT 
GGGACACCAC TCACCTCAGA TCAGATCTTT GTGCAGCTTG AGAAAATCTG GAACTCCTCA 
CTGCAATCCA ACAAGGAGCC TGTTGGCATC CTAACCTCCA ACCACCGGAA CAGCTGGGCC 
AAGGCCTACA GCAGCCTCAT TAAAGACAAA GTAAACCGGG AGTCGGTGAA CTCCATCCAG 
AAGAGCATCT TCACTGTGTG CCTGGACAAG CAAGTGCCCA GAGTCTCAGA TGATGTCTAC 
CGCAGCCACG TAGCCGGCCA GATGCTTCAT GGTGGTGGCA GCAAGTTCAA CAGTGGCAAC 
CGCTGGTTCG ACAAGACACT GCAGTTTATT GTGGCAGAAG ATGGCTCCTG TGGGATGGTT 
TATGAACATG CAGCTGCAGA AGGGCCCCCC ATTGTCGCTC TTGTGGACCA TGTCATGGAG 
TATACAAAGA AGCCTGAACT TGTGCGGTCC CCTATGGTAC CCCTGCCTAT GCCCAAGAAG 
CTGCGGTTCA ACATCACACC TGAGATCAAG AATGACATAG AGAAGGCCAA ACAGAACATC 
AGCATCATGA TCCAGGACCT GGACATCATG ATGCTGGTTT TCCATCACTT TGGAAAGGAC 
TTCCCCAAGT CCCAGAAGCT AAGCCCTGAT GCCTTCATCC AGATAGCCCT GCAGCTGGCA 
TACTACAGGA TCTATGGGCA GGCGTGTGCC ACGTATGAAA GCGCCTCTCT GCGCATGTTT 
CACCTGGGCC GCACTGACAC CATCCGCTCC GCCTCCACAG ACTCGCTGGC CTTTGTCAAA 
GGCATGGATG ACCCCAAAGT GCCGGAGCAG CAGAGGGTGG AGCTGCTTCG AAAGGCTGTG 
CAGGCCCACC GAGCCTACAC CGACCGCGCC ATCCGAGGGG AGGCCTTTGA CCGGCACTTG 
CTGGGTCTGA AGCTACAGGC CATCGAGGAC CTGGTGAGCA TGCCTGACAT CTTCATGGAT 
ACCTCCTACG CCATTGCCAT GCACTTCAAC CTTTCTACCA GCCAGGTCCC TGCCAAGACA 
GACTGTGTCA TGTCCTTCGG ACCTGTGGTC CCAGACGGTT ACGGCATCTG CTACAATCCC 
ATGGAGGCCC ATATCAACTT TTCCGTGTCA GCCTACAACA GCTGTGCTGA GACCAACGCT 
GCCCGCATGG CTCACTACTT GGAGAAAGCT CTGCTGGACA TGCGCACCTT ACTCCAGAAC 
CACCCCAGGG CCAAGCTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Crat ( XM_017591845.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017447334.1 Rattus norvegicus carnitine O-acetyltransferase (Crat), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ORa25607
Clone ID Related Accession (Same CDS sequence)	XM_017591845.1 , XM_008761664.1
Accession Version	XM_017591845.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1818bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	carnitine O-acetyltransferase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005102.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGTGT CTGGTCGATT TAAGGCCCAC CAGGATGCAC TGCCACGGCT GCCTGTGCCT 
CCACTTCAGC AATCCCTGGA CCATTACCTC AAGGCACTGC AGCCCATCGT GAGTGAGGAA 
GAATGGGCTC ACACCAAGCA ACTGGTGGAC GAGTTTCAGA CCTCAGGGGG TGTAGGGGAA 
CGCCTGCAGA AGGGATTGGA GCGCAGGGCC AAGAAAATGG AGAACTGGCT GTCCGAGTGG 
TGGCTCAAGA CAGCCTATCT CCAGTTCCGC CAGCCTGTGG TCATCTACTC CAGTCCAGGA 
GTGCTGCTGC CCAAGCAGGA CTTCATGGAT CTACAGGGTC AGCTCCGGTT TGCTGCCAAA 
CTCATCGAGG GTGTATTGGA TTTCAAGTCC ATGATTGACA ACGAGACGCT GCCAGTTGAG 
TTCCTGGGAG GACAGCCACT GTGCATGAAC CAGTATTATC AGATCCTGTC TTCTTGCCGT 
GTGCCTGGCC TCAAGCAGGA CTCAGTGGTG AACTTCTTAA AGTCAAAGAA ACCACCCACA 
CATATAACCG TGGTGCATAA CTACCAGTTC TTCGAGCTGG ATGTGTACCA CAGTGATGGG 
ACACCACTCA CCTCAGATCA GATCTTTGTG CAGCTTGAGA AAATCTGGAA CTCCTCACTG 
CAATCCAACA AGGAGCCTGT TGGCATCCTA ACCTCCAACC ACCGGAACAG CTGGGCCAAG 
GCCTACAGCA GCCTCATTAA AGACAAAGTA AACCGGGAGT CGGTGAACTC CATCCAGAAG 
AGCATCTTCA CTGTGTGCCT GGACAAGCAA GTGCCCAGAG TCTCAGATGA TGTCTACCGC 
AGCCACGTAG CCGGCCAGAT GCTTCATGGT GGTGGCAGCA AGTTCAACAG TGGCAACCGC 
TGGTTCGACA AGACACTGCA GTTTATTGTG GCAGAAGATG GCTCCTGTGG GATGGTTTAT 
GAACATGCAG CTGCAGAAGG GCCCCCCATT GTCGCTCTTG TGGACCATGT CATGGAGTAT 
ACAAAGAAGC CTGAACTTGT GCGGTCCCCT ATGGTACCCC TGCCTATGCC CAAGAAGCTG 
CGGTTCAACA TCACACCTGA GATCAAGAAT GACATAGAGA AGGCCAAACA GAACATCAGC 
ATCATGATCC AGGACCTGGA CATCATGATG CTGGTTTTCC ATCACTTTGG AAAGGACTTC 
CCCAAGTCCC AGAAGCTAAG CCCTGATGCC TTCATCCAGA TAGCCCTGCA GCTGGCATAC 
TACAGGATCT ATGGGCAGGC GTGTGCCACG TATGAAAGCG CCTCTCTGCG CATGTTTCAC 
CTGGGCCGCA CTGACACCAT CCGCTCCGCC TCCACAGACT CGCTGGCCTT TGTCAAAGGC 
ATGGATGACC CCAAAGTGCC GGAGCAGCAG AGGGTGGAGC TGCTTCGAAA GGCTGTGCAG 
GCCCACCGAG CCTACACCGA CCGCGCCATC CGAGGGGAGG CCTTTGACCG GCACTTGCTG 
GGTCTGAAGC TACAGGCCAT CGAGGACCTG GTGAGCATGC CTGACATCTT CATGGATACC 
TCCTACGCCA TTGCCATGCA CTTCAACCTT TCTACCAGCC AGGTCCCTGC CAAGACAGAC 
TGTGTCATGT CCTTCGGACC TGTGGTCCCA GACGGTTACG GCATCTGCTA CAATCCCATG 
GAGGCCCATA TCAACTTTTC CGTGTCAGCC TACAACAGCT GTGCTGAGAC CAACGCTGCC 
CGCATGGCTC ACTACTTGGA GAAAGCTCTG CTGGACATGC GCACCTTACT CCAGAACCAC 
CCCAGGGCCA AGCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017447334.1
CDS	185..2002
Translation	MKVSGRFKAHQDALPRLPVPPLQQSLDHYLKALQPIVSEEEWAHTKQLVDEFQTSGGVGERLQKGLERRAKKMENWLSEWWLKTAYLQFRQPVVIYSSPGVLLPKQDFMDLQGQLRFAAKLIEGVLDFKSMIDNETLPVEFLGGQPLCMNQYYQILSSCRVPGLKQDSVVNFLKSKKPPTHITVVHNYQFFELDVYHSDGTPLTSDQIFVQLEKIWNSSLQSNKEPVGILTSNHRNSWAKAYSSLIKDKVNRESVNSIQKSIFTVCLDKQVPRVSDDVYRSHVAGQMLHGGGSKFNSGNRWFDKTLQFIVAEDGSCGMVYEHAAAEGPPIVALVDHVMEYTKKPELVRSPMVPLPMPKKLRFNITPEIKNDIEKAKQNISIMIQDLDIMMLVFHHFGKDFPKSQKLSPDAFIQIALQLAYYRIYGQACATYESASLRMFHLGRTDTIRSASTDSLAFVKGMDDPKVPEQQRVELLRKAVQAHRAYTDRAIRGEAFDRHLLGLKLQAIEDLVSMPDIFMDTSYAIAMHFNLSTSQVPAKTDCVMSFGPVVPDGYGICYNPMEAHINFSVSAYNSCAETNAARMAHYLEKALLDMRTLLQNHPRAKL

Target ORF information:

RefSeq Version	XM_017591845.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus carnitine O-acetyltransferase (Crat), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017591845.1

ORF Insert Sequence:

ATGAAGGTGT CTGGTCGATT TAAGGCCCAC CAGGATGCAC TGCCACGGCT GCCTGTGCCT 
CCACTTCAGC AATCCCTGGA CCATTACCTC AAGGCACTGC AGCCCATCGT GAGTGAGGAA 
GAATGGGCTC ACACCAAGCA ACTGGTGGAC GAGTTTCAGA CCTCAGGGGG TGTAGGGGAA 
CGCCTGCAGA AGGGATTGGA GCGCAGGGCC AAGAAAATGG AGAACTGGCT GTCCGAGTGG 
TGGCTCAAGA CAGCCTATCT CCAGTTCCGC CAGCCTGTGG TCATCTACTC CAGTCCAGGA 
GTGCTGCTGC CCAAGCAGGA CTTCATGGAT CTACAGGGTC AGCTCCGGTT TGCTGCCAAA 
CTCATCGAGG GTGTATTGGA TTTCAAGTCC ATGATTGACA ACGAGACGCT GCCAGTTGAG 
TTCCTGGGAG GACAGCCACT GTGCATGAAC CAGTATTATC AGATCCTGTC TTCTTGCCGT 
GTGCCTGGCC TCAAGCAGGA CTCAGTGGTG AACTTCTTAA AGTCAAAGAA ACCACCCACA 
CATATAACCG TGGTGCATAA CTACCAGTTC TTCGAGCTGG ATGTGTACCA CAGTGATGGG 
ACACCACTCA CCTCAGATCA GATCTTTGTG CAGCTTGAGA AAATCTGGAA CTCCTCACTG 
CAATCCAACA AGGAGCCTGT TGGCATCCTA ACCTCCAACC ACCGGAACAG CTGGGCCAAG 
GCCTACAGCA GCCTCATTAA AGACAAAGTA AACCGGGAGT CGGTGAACTC CATCCAGAAG 
AGCATCTTCA CTGTGTGCCT GGACAAGCAA GTGCCCAGAG TCTCAGATGA TGTCTACCGC 
AGCCACGTAG CCGGCCAGAT GCTTCATGGT GGTGGCAGCA AGTTCAACAG TGGCAACCGC 
TGGTTCGACA AGACACTGCA GTTTATTGTG GCAGAAGATG GCTCCTGTGG GATGGTTTAT 
GAACATGCAG CTGCAGAAGG GCCCCCCATT GTCGCTCTTG TGGACCATGT CATGGAGTAT 
ACAAAGAAGC CTGAACTTGT GCGGTCCCCT ATGGTACCCC TGCCTATGCC CAAGAAGCTG 
CGGTTCAACA TCACACCTGA GATCAAGAAT GACATAGAGA AGGCCAAACA GAACATCAGC 
ATCATGATCC AGGACCTGGA CATCATGATG CTGGTTTTCC ATCACTTTGG AAAGGACTTC 
CCCAAGTCCC AGAAGCTAAG CCCTGATGCC TTCATCCAGA TAGCCCTGCA GCTGGCATAC 
TACAGGATCT ATGGGCAGGC GTGTGCCACG TATGAAAGCG CCTCTCTGCG CATGTTTCAC 
CTGGGCCGCA CTGACACCAT CCGCTCCGCC TCCACAGACT CGCTGGCCTT TGTCAAAGGC 
ATGGATGACC CCAAAGTGCC GGAGCAGCAG AGGGTGGAGC TGCTTCGAAA GGCTGTGCAG 
GCCCACCGAG CCTACACCGA CCGCGCCATC CGAGGGGAGG CCTTTGACCG GCACTTGCTG 
GGTCTGAAGC TACAGGCCAT CGAGGACCTG GTGAGCATGC CTGACATCTT CATGGATACC 
TCCTACGCCA TTGCCATGCA CTTCAACCTT TCTACCAGCC AGGTCCCTGC CAAGACAGAC 
TGTGTCATGT CCTTCGGACC TGTGGTCCCA GACGGTTACG GCATCTGCTA CAATCCCATG 
GAGGCCCATA TCAACTTTTC CGTGTCAGCC TACAACAGCT GTGCTGAGAC CAACGCTGCC 
CGCATGGCTC ACTACTTGGA GAAAGCTCTG CTGGACATGC GCACCTTACT CCAGAACCAC 
CCCAGGGCCA AGCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Crat ( XM_008761664.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008759886.1 Rattus norvegicus carnitine O-acetyltransferase (Crat), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ORa25607
Clone ID Related Accession (Same CDS sequence)	XM_017591845.1 , XM_008761664.1
Accession Version	XM_008761664.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1818bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	carnitine O-acetyltransferase isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005102.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGGTGT CTGGTCGATT TAAGGCCCAC CAGGATGCAC TGCCACGGCT GCCTGTGCCT 
CCACTTCAGC AATCCCTGGA CCATTACCTC AAGGCACTGC AGCCCATCGT GAGTGAGGAA 
GAATGGGCTC ACACCAAGCA ACTGGTGGAC GAGTTTCAGA CCTCAGGGGG TGTAGGGGAA 
CGCCTGCAGA AGGGATTGGA GCGCAGGGCC AAGAAAATGG AGAACTGGCT GTCCGAGTGG 
TGGCTCAAGA CAGCCTATCT CCAGTTCCGC CAGCCTGTGG TCATCTACTC CAGTCCAGGA 
GTGCTGCTGC CCAAGCAGGA CTTCATGGAT CTACAGGGTC AGCTCCGGTT TGCTGCCAAA 
CTCATCGAGG GTGTATTGGA TTTCAAGTCC ATGATTGACA ACGAGACGCT GCCAGTTGAG 
TTCCTGGGAG GACAGCCACT GTGCATGAAC CAGTATTATC AGATCCTGTC TTCTTGCCGT 
GTGCCTGGCC TCAAGCAGGA CTCAGTGGTG AACTTCTTAA AGTCAAAGAA ACCACCCACA 
CATATAACCG TGGTGCATAA CTACCAGTTC TTCGAGCTGG ATGTGTACCA CAGTGATGGG 
ACACCACTCA CCTCAGATCA GATCTTTGTG CAGCTTGAGA AAATCTGGAA CTCCTCACTG 
CAATCCAACA AGGAGCCTGT TGGCATCCTA ACCTCCAACC ACCGGAACAG CTGGGCCAAG 
GCCTACAGCA GCCTCATTAA AGACAAAGTA AACCGGGAGT CGGTGAACTC CATCCAGAAG 
AGCATCTTCA CTGTGTGCCT GGACAAGCAA GTGCCCAGAG TCTCAGATGA TGTCTACCGC 
AGCCACGTAG CCGGCCAGAT GCTTCATGGT GGTGGCAGCA AGTTCAACAG TGGCAACCGC 
TGGTTCGACA AGACACTGCA GTTTATTGTG GCAGAAGATG GCTCCTGTGG GATGGTTTAT 
GAACATGCAG CTGCAGAAGG GCCCCCCATT GTCGCTCTTG TGGACCATGT CATGGAGTAT 
ACAAAGAAGC CTGAACTTGT GCGGTCCCCT ATGGTACCCC TGCCTATGCC CAAGAAGCTG 
CGGTTCAACA TCACACCTGA GATCAAGAAT GACATAGAGA AGGCCAAACA GAACATCAGC 
ATCATGATCC AGGACCTGGA CATCATGATG CTGGTTTTCC ATCACTTTGG AAAGGACTTC 
CCCAAGTCCC AGAAGCTAAG CCCTGATGCC TTCATCCAGA TAGCCCTGCA GCTGGCATAC 
TACAGGATCT ATGGGCAGGC GTGTGCCACG TATGAAAGCG CCTCTCTGCG CATGTTTCAC 
CTGGGCCGCA CTGACACCAT CCGCTCCGCC TCCACAGACT CGCTGGCCTT TGTCAAAGGC 
ATGGATGACC CCAAAGTGCC GGAGCAGCAG AGGGTGGAGC TGCTTCGAAA GGCTGTGCAG 
GCCCACCGAG CCTACACCGA CCGCGCCATC CGAGGGGAGG CCTTTGACCG GCACTTGCTG 
GGTCTGAAGC TACAGGCCAT CGAGGACCTG GTGAGCATGC CTGACATCTT CATGGATACC 
TCCTACGCCA TTGCCATGCA CTTCAACCTT TCTACCAGCC AGGTCCCTGC CAAGACAGAC 
TGTGTCATGT CCTTCGGACC TGTGGTCCCA GACGGTTACG GCATCTGCTA CAATCCCATG 
GAGGCCCATA TCAACTTTTC CGTGTCAGCC TACAACAGCT GTGCTGAGAC CAACGCTGCC 
CGCATGGCTC ACTACTTGGA GAAAGCTCTG CTGGACATGC GCACCTTACT CCAGAACCAC 
CCCAGGGCCA AGCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008759886.1
CDS	155..1972
Translation	MKVSGRFKAHQDALPRLPVPPLQQSLDHYLKALQPIVSEEEWAHTKQLVDEFQTSGGVGERLQKGLERRAKKMENWLSEWWLKTAYLQFRQPVVIYSSPGVLLPKQDFMDLQGQLRFAAKLIEGVLDFKSMIDNETLPVEFLGGQPLCMNQYYQILSSCRVPGLKQDSVVNFLKSKKPPTHITVVHNYQFFELDVYHSDGTPLTSDQIFVQLEKIWNSSLQSNKEPVGILTSNHRNSWAKAYSSLIKDKVNRESVNSIQKSIFTVCLDKQVPRVSDDVYRSHVAGQMLHGGGSKFNSGNRWFDKTLQFIVAEDGSCGMVYEHAAAEGPPIVALVDHVMEYTKKPELVRSPMVPLPMPKKLRFNITPEIKNDIEKAKQNISIMIQDLDIMMLVFHHFGKDFPKSQKLSPDAFIQIALQLAYYRIYGQACATYESASLRMFHLGRTDTIRSASTDSLAFVKGMDDPKVPEQQRVELLRKAVQAHRAYTDRAIRGEAFDRHLLGLKLQAIEDLVSMPDIFMDTSYAIAMHFNLSTSQVPAKTDCVMSFGPVVPDGYGICYNPMEAHINFSVSAYNSCAETNAARMAHYLEKALLDMRTLLQNHPRAKL

Target ORF information:

RefSeq Version	XM_008761664.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus carnitine O-acetyltransferase (Crat), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008761664.1

ORF Insert Sequence:

ATGAAGGTGT CTGGTCGATT TAAGGCCCAC CAGGATGCAC TGCCACGGCT GCCTGTGCCT 
CCACTTCAGC AATCCCTGGA CCATTACCTC AAGGCACTGC AGCCCATCGT GAGTGAGGAA 
GAATGGGCTC ACACCAAGCA ACTGGTGGAC GAGTTTCAGA CCTCAGGGGG TGTAGGGGAA 
CGCCTGCAGA AGGGATTGGA GCGCAGGGCC AAGAAAATGG AGAACTGGCT GTCCGAGTGG 
TGGCTCAAGA CAGCCTATCT CCAGTTCCGC CAGCCTGTGG TCATCTACTC CAGTCCAGGA 
GTGCTGCTGC CCAAGCAGGA CTTCATGGAT CTACAGGGTC AGCTCCGGTT TGCTGCCAAA 
CTCATCGAGG GTGTATTGGA TTTCAAGTCC ATGATTGACA ACGAGACGCT GCCAGTTGAG 
TTCCTGGGAG GACAGCCACT GTGCATGAAC CAGTATTATC AGATCCTGTC TTCTTGCCGT 
GTGCCTGGCC TCAAGCAGGA CTCAGTGGTG AACTTCTTAA AGTCAAAGAA ACCACCCACA 
CATATAACCG TGGTGCATAA CTACCAGTTC TTCGAGCTGG ATGTGTACCA CAGTGATGGG 
ACACCACTCA CCTCAGATCA GATCTTTGTG CAGCTTGAGA AAATCTGGAA CTCCTCACTG 
CAATCCAACA AGGAGCCTGT TGGCATCCTA ACCTCCAACC ACCGGAACAG CTGGGCCAAG 
GCCTACAGCA GCCTCATTAA AGACAAAGTA AACCGGGAGT CGGTGAACTC CATCCAGAAG 
AGCATCTTCA CTGTGTGCCT GGACAAGCAA GTGCCCAGAG TCTCAGATGA TGTCTACCGC 
AGCCACGTAG CCGGCCAGAT GCTTCATGGT GGTGGCAGCA AGTTCAACAG TGGCAACCGC 
TGGTTCGACA AGACACTGCA GTTTATTGTG GCAGAAGATG GCTCCTGTGG GATGGTTTAT 
GAACATGCAG CTGCAGAAGG GCCCCCCATT GTCGCTCTTG TGGACCATGT CATGGAGTAT 
ACAAAGAAGC CTGAACTTGT GCGGTCCCCT ATGGTACCCC TGCCTATGCC CAAGAAGCTG 
CGGTTCAACA TCACACCTGA GATCAAGAAT GACATAGAGA AGGCCAAACA GAACATCAGC 
ATCATGATCC AGGACCTGGA CATCATGATG CTGGTTTTCC ATCACTTTGG AAAGGACTTC 
CCCAAGTCCC AGAAGCTAAG CCCTGATGCC TTCATCCAGA TAGCCCTGCA GCTGGCATAC 
TACAGGATCT ATGGGCAGGC GTGTGCCACG TATGAAAGCG CCTCTCTGCG CATGTTTCAC 
CTGGGCCGCA CTGACACCAT CCGCTCCGCC TCCACAGACT CGCTGGCCTT TGTCAAAGGC 
ATGGATGACC CCAAAGTGCC GGAGCAGCAG AGGGTGGAGC TGCTTCGAAA GGCTGTGCAG 
GCCCACCGAG CCTACACCGA CCGCGCCATC CGAGGGGAGG CCTTTGACCG GCACTTGCTG 
GGTCTGAAGC TACAGGCCAT CGAGGACCTG GTGAGCATGC CTGACATCTT CATGGATACC 
TCCTACGCCA TTGCCATGCA CTTCAACCTT TCTACCAGCC AGGTCCCTGC CAAGACAGAC 
TGTGTCATGT CCTTCGGACC TGTGGTCCCA GACGGTTACG GCATCTGCTA CAATCCCATG 
GAGGCCCATA TCAACTTTTC CGTGTCAGCC TACAACAGCT GTGCTGAGAC CAACGCTGCC 
CGCATGGCTC ACTACTTGGA GAAAGCTCTG CTGGACATGC GCACCTTACT CCAGAACCAC 
CCCAGGGCCA AGCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Crat ( XM_008761663.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008759885.1 Rattus norvegicus carnitine O-acetyltransferase (Crat), transcript variant X2, mRNA.

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CloneID	ORa25606
Clone ID Related Accession (Same CDS sequence)	XM_008761663.2 , XM_006233823.2
Accession Version	XM_008761663.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1881bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	carnitine O-acetyltransferase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005102.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008761663.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGACG AGCAGCAGAG AGAGAAGGTG AAACCCCTGG GCCTCCTCAA GCCCTCCTCC 
TTGATGAAGG TGTCTGGTCG ATTTAAGGCC CACCAGGATG CACTGCCACG GCTGCCTGTG 
CCTCCACTTC AGCAATCCCT GGACCATTAC CTCAAGGCAC TGCAGCCCAT CGTGAGTGAG 
GAAGAATGGG CTCACACCAA GCAACTGGTG GACGAGTTTC AGACCTCAGG GGGTGTAGGG 
GAACGCCTGC AGAAGGGATT GGAGCGCAGG GCCAAGAAAA TGGAGAACTG GCTGTCCGAG 
TGGTGGCTCA AGACAGCCTA TCTCCAGTTC CGCCAGCCTG TGGTCATCTA CTCCAGTCCA 
GGAGTGCTGC TGCCCAAGCA GGACTTCATG GATCTACAGG GTCAGCTCCG GTTTGCTGCC 
AAACTCATCG AGGGTGTATT GGATTTCAAG TCCATGATTG ACAACGAGAC GCTGCCAGTT 
GAGTTCCTGG GAGGACAGCC ACTGTGCATG AACCAGTATT ATCAGATCCT GTCTTCTTGC 
CGTGTGCCTG GCCTCAAGCA GGACTCAGTG GTGAACTTCT TAAAGTCAAA GAAACCACCC 
ACACATATAA CCGTGGTGCA TAACTACCAG TTCTTCGAGC TGGATGTGTA CCACAGTGAT 
GGGACACCAC TCACCTCAGA TCAGATCTTT GTGCAGCTTG AGAAAATCTG GAACTCCTCA 
CTGCAATCCA ACAAGGAGCC TGTTGGCATC CTAACCTCCA ACCACCGGAA CAGCTGGGCC 
AAGGCCTACA GCAGCCTCAT TAAAGACAAA GTAAACCGGG AGTCGGTGAA CTCCATCCAG 
AAGAGCATCT TCACTGTGTG CCTGGACAAG CAAGTGCCCA GAGTCTCAGA TGATGTCTAC 
CGCAGCCACG TAGCCGGCCA GATGCTTCAT GGTGGTGGCA GCAAGTTCAA CAGTGGCAAC 
CGCTGGTTCG ACAAGACACT GCAGTTTATT GTGGCAGAAG ATGGCTCCTG TGGGATGGTT 
TATGAACATG CAGCTGCAGA AGGGCCCCCC ATTGTCGCTC TTGTGGACCA TGTCATGGAG 
TATACAAAGA AGCCTGAACT TGTGCGGTCC CCTATGGTAC CCCTGCCTAT GCCCAAGAAG 
CTGCGGTTCA ACATCACACC TGAGATCAAG AATGACATAG AGAAGGCCAA ACAGAACATC 
AGCATCATGA TCCAGGACCT GGACATCATG ATGCTGGTTT TCCATCACTT TGGAAAGGAC 
TTCCCCAAGT CCCAGAAGCT AAGCCCTGAT GCCTTCATCC AGATAGCCCT GCAGCTGGCA 
TACTACAGGA TCTATGGGCA GGCGTGTGCC ACGTATGAAA GCGCCTCTCT GCGCATGTTT 
CACCTGGGCC GCACTGACAC CATCCGCTCC GCCTCCACAG ACTCGCTGGC CTTTGTCAAA 
GGCATGGATG ACCCCAAAGT GCCGGAGCAG CAGAGGGTGG AGCTGCTTCG AAAGGCTGTG 
CAGGCCCACC GAGCCTACAC CGACCGCGCC ATCCGAGGGG AGGCCTTTGA CCGGCACTTG 
CTGGGTCTGA AGCTACAGGC CATCGAGGAC CTGGTGAGCA TGCCTGACAT CTTCATGGAT 
ACCTCCTACG CCATTGCCAT GCACTTCAAC CTTTCTACCA GCCAGGTCCC TGCCAAGACA 
GACTGTGTCA TGTCCTTCGG ACCTGTGGTC CCAGACGGTT ACGGCATCTG CTACAATCCC 
ATGGAGGCCC ATATCAACTT TTCCGTGTCA GCCTACAACA GCTGTGCTGA GACCAACGCT 
GCCCGCATGG CTCACTACTT GGAGAAAGCT CTGCTGGACA TGCGCACCTT ACTCCAGAAC 
CACCCCAGGG CCAAGCTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008759885.1
CDS	224..2104
Translation	MEDEQQREKVKPLGLLKPSSLMKVSGRFKAHQDALPRLPVPPLQQSLDHYLKALQPIVSEEEWAHTKQLVDEFQTSGGVGERLQKGLERRAKKMENWLSEWWLKTAYLQFRQPVVIYSSPGVLLPKQDFMDLQGQLRFAAKLIEGVLDFKSMIDNETLPVEFLGGQPLCMNQYYQILSSCRVPGLKQDSVVNFLKSKKPPTHITVVHNYQFFELDVYHSDGTPLTSDQIFVQLEKIWNSSLQSNKEPVGILTSNHRNSWAKAYSSLIKDKVNRESVNSIQKSIFTVCLDKQVPRVSDDVYRSHVAGQMLHGGGSKFNSGNRWFDKTLQFIVAEDGSCGMVYEHAAAEGPPIVALVDHVMEYTKKPELVRSPMVPLPMPKKLRFNITPEIKNDIEKAKQNISIMIQDLDIMMLVFHHFGKDFPKSQKLSPDAFIQIALQLAYYRIYGQACATYESASLRMFHLGRTDTIRSASTDSLAFVKGMDDPKVPEQQRVELLRKAVQAHRAYTDRAIRGEAFDRHLLGLKLQAIEDLVSMPDIFMDTSYAIAMHFNLSTSQVPAKTDCVMSFGPVVPDGYGICYNPMEAHINFSVSAYNSCAETNAARMAHYLEKALLDMRTLLQNHPRAKL

Target ORF information:

RefSeq Version	XM_008761663.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus carnitine O-acetyltransferase (Crat), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008761663.2

ORF Insert Sequence:

ATGGAGGACG AGCAGCAGAG AGAGAAGGTG AAACCCCTGG GCCTCCTCAA GCCCTCCTCC 
TTGATGAAGG TGTCTGGTCG ATTTAAGGCC CACCAGGATG CACTGCCACG GCTGCCTGTG 
CCTCCACTTC AGCAATCCCT GGACCATTAC CTCAAGGCAC TGCAGCCCAT CGTGAGTGAG 
GAAGAATGGG CTCACACCAA GCAACTGGTG GACGAGTTTC AGACCTCAGG GGGTGTAGGG 
GAACGCCTGC AGAAGGGATT GGAGCGCAGG GCCAAGAAAA TGGAGAACTG GCTGTCCGAG 
TGGTGGCTCA AGACAGCCTA TCTCCAGTTC CGCCAGCCTG TGGTCATCTA CTCCAGTCCA 
GGAGTGCTGC TGCCCAAGCA GGACTTCATG GATCTACAGG GTCAGCTCCG GTTTGCTGCC 
AAACTCATCG AGGGTGTATT GGATTTCAAG TCCATGATTG ACAACGAGAC GCTGCCAGTT 
GAGTTCCTGG GAGGACAGCC ACTGTGCATG AACCAGTATT ATCAGATCCT GTCTTCTTGC 
CGTGTGCCTG GCCTCAAGCA GGACTCAGTG GTGAACTTCT TAAAGTCAAA GAAACCACCC 
ACACATATAA CCGTGGTGCA TAACTACCAG TTCTTCGAGC TGGATGTGTA CCACAGTGAT 
GGGACACCAC TCACCTCAGA TCAGATCTTT GTGCAGCTTG AGAAAATCTG GAACTCCTCA 
CTGCAATCCA ACAAGGAGCC TGTTGGCATC CTAACCTCCA ACCACCGGAA CAGCTGGGCC 
AAGGCCTACA GCAGCCTCAT TAAAGACAAA GTAAACCGGG AGTCGGTGAA CTCCATCCAG 
AAGAGCATCT TCACTGTGTG CCTGGACAAG CAAGTGCCCA GAGTCTCAGA TGATGTCTAC 
CGCAGCCACG TAGCCGGCCA GATGCTTCAT GGTGGTGGCA GCAAGTTCAA CAGTGGCAAC 
CGCTGGTTCG ACAAGACACT GCAGTTTATT GTGGCAGAAG ATGGCTCCTG TGGGATGGTT 
TATGAACATG CAGCTGCAGA AGGGCCCCCC ATTGTCGCTC TTGTGGACCA TGTCATGGAG 
TATACAAAGA AGCCTGAACT TGTGCGGTCC CCTATGGTAC CCCTGCCTAT GCCCAAGAAG 
CTGCGGTTCA ACATCACACC TGAGATCAAG AATGACATAG AGAAGGCCAA ACAGAACATC 
AGCATCATGA TCCAGGACCT GGACATCATG ATGCTGGTTT TCCATCACTT TGGAAAGGAC 
TTCCCCAAGT CCCAGAAGCT AAGCCCTGAT GCCTTCATCC AGATAGCCCT GCAGCTGGCA 
TACTACAGGA TCTATGGGCA GGCGTGTGCC ACGTATGAAA GCGCCTCTCT GCGCATGTTT 
CACCTGGGCC GCACTGACAC CATCCGCTCC GCCTCCACAG ACTCGCTGGC CTTTGTCAAA 
GGCATGGATG ACCCCAAAGT GCCGGAGCAG CAGAGGGTGG AGCTGCTTCG AAAGGCTGTG 
CAGGCCCACC GAGCCTACAC CGACCGCGCC ATCCGAGGGG AGGCCTTTGA CCGGCACTTG 
CTGGGTCTGA AGCTACAGGC CATCGAGGAC CTGGTGAGCA TGCCTGACAT CTTCATGGAT 
ACCTCCTACG CCATTGCCAT GCACTTCAAC CTTTCTACCA GCCAGGTCCC TGCCAAGACA 
GACTGTGTCA TGTCCTTCGG ACCTGTGGTC CCAGACGGTT ACGGCATCTG CTACAATCCC 
ATGGAGGCCC ATATCAACTT TTCCGTGTCA GCCTACAACA GCTGTGCTGA GACCAACGCT 
GCCCGCATGG CTCACTACTT GGAGAAAGCT CTGCTGGACA TGCGCACCTT ACTCCAGAAC 
CACCCCAGGG CCAAGCTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Crat ( XM_006233823.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006233885.1 Rattus norvegicus carnitine O-acetyltransferase (Crat), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa25606
Clone ID Related Accession (Same CDS sequence)	XM_008761663.2 , XM_006233823.2
Accession Version	XM_006233823.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1881bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	carnitine O-acetyltransferase isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005102.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006233823.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGACG AGCAGCAGAG AGAGAAGGTG AAACCCCTGG GCCTCCTCAA GCCCTCCTCC 
TTGATGAAGG TGTCTGGTCG ATTTAAGGCC CACCAGGATG CACTGCCACG GCTGCCTGTG 
CCTCCACTTC AGCAATCCCT GGACCATTAC CTCAAGGCAC TGCAGCCCAT CGTGAGTGAG 
GAAGAATGGG CTCACACCAA GCAACTGGTG GACGAGTTTC AGACCTCAGG GGGTGTAGGG 
GAACGCCTGC AGAAGGGATT GGAGCGCAGG GCCAAGAAAA TGGAGAACTG GCTGTCCGAG 
TGGTGGCTCA AGACAGCCTA TCTCCAGTTC CGCCAGCCTG TGGTCATCTA CTCCAGTCCA 
GGAGTGCTGC TGCCCAAGCA GGACTTCATG GATCTACAGG GTCAGCTCCG GTTTGCTGCC 
AAACTCATCG AGGGTGTATT GGATTTCAAG TCCATGATTG ACAACGAGAC GCTGCCAGTT 
GAGTTCCTGG GAGGACAGCC ACTGTGCATG AACCAGTATT ATCAGATCCT GTCTTCTTGC 
CGTGTGCCTG GCCTCAAGCA GGACTCAGTG GTGAACTTCT TAAAGTCAAA GAAACCACCC 
ACACATATAA CCGTGGTGCA TAACTACCAG TTCTTCGAGC TGGATGTGTA CCACAGTGAT 
GGGACACCAC TCACCTCAGA TCAGATCTTT GTGCAGCTTG AGAAAATCTG GAACTCCTCA 
CTGCAATCCA ACAAGGAGCC TGTTGGCATC CTAACCTCCA ACCACCGGAA CAGCTGGGCC 
AAGGCCTACA GCAGCCTCAT TAAAGACAAA GTAAACCGGG AGTCGGTGAA CTCCATCCAG 
AAGAGCATCT TCACTGTGTG CCTGGACAAG CAAGTGCCCA GAGTCTCAGA TGATGTCTAC 
CGCAGCCACG TAGCCGGCCA GATGCTTCAT GGTGGTGGCA GCAAGTTCAA CAGTGGCAAC 
CGCTGGTTCG ACAAGACACT GCAGTTTATT GTGGCAGAAG ATGGCTCCTG TGGGATGGTT 
TATGAACATG CAGCTGCAGA AGGGCCCCCC ATTGTCGCTC TTGTGGACCA TGTCATGGAG 
TATACAAAGA AGCCTGAACT TGTGCGGTCC CCTATGGTAC CCCTGCCTAT GCCCAAGAAG 
CTGCGGTTCA ACATCACACC TGAGATCAAG AATGACATAG AGAAGGCCAA ACAGAACATC 
AGCATCATGA TCCAGGACCT GGACATCATG ATGCTGGTTT TCCATCACTT TGGAAAGGAC 
TTCCCCAAGT CCCAGAAGCT AAGCCCTGAT GCCTTCATCC AGATAGCCCT GCAGCTGGCA 
TACTACAGGA TCTATGGGCA GGCGTGTGCC ACGTATGAAA GCGCCTCTCT GCGCATGTTT 
CACCTGGGCC GCACTGACAC CATCCGCTCC GCCTCCACAG ACTCGCTGGC CTTTGTCAAA 
GGCATGGATG ACCCCAAAGT GCCGGAGCAG CAGAGGGTGG AGCTGCTTCG AAAGGCTGTG 
CAGGCCCACC GAGCCTACAC CGACCGCGCC ATCCGAGGGG AGGCCTTTGA CCGGCACTTG 
CTGGGTCTGA AGCTACAGGC CATCGAGGAC CTGGTGAGCA TGCCTGACAT CTTCATGGAT 
ACCTCCTACG CCATTGCCAT GCACTTCAAC CTTTCTACCA GCCAGGTCCC TGCCAAGACA 
GACTGTGTCA TGTCCTTCGG ACCTGTGGTC CCAGACGGTT ACGGCATCTG CTACAATCCC 
ATGGAGGCCC ATATCAACTT TTCCGTGTCA GCCTACAACA GCTGTGCTGA GACCAACGCT 
GCCCGCATGG CTCACTACTT GGAGAAAGCT CTGCTGGACA TGCGCACCTT ACTCCAGAAC 
CACCCCAGGG CCAAGCTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006233885.1
CDS	735..2615
Translation	MEDEQQREKVKPLGLLKPSSLMKVSGRFKAHQDALPRLPVPPLQQSLDHYLKALQPIVSEEEWAHTKQLVDEFQTSGGVGERLQKGLERRAKKMENWLSEWWLKTAYLQFRQPVVIYSSPGVLLPKQDFMDLQGQLRFAAKLIEGVLDFKSMIDNETLPVEFLGGQPLCMNQYYQILSSCRVPGLKQDSVVNFLKSKKPPTHITVVHNYQFFELDVYHSDGTPLTSDQIFVQLEKIWNSSLQSNKEPVGILTSNHRNSWAKAYSSLIKDKVNRESVNSIQKSIFTVCLDKQVPRVSDDVYRSHVAGQMLHGGGSKFNSGNRWFDKTLQFIVAEDGSCGMVYEHAAAEGPPIVALVDHVMEYTKKPELVRSPMVPLPMPKKLRFNITPEIKNDIEKAKQNISIMIQDLDIMMLVFHHFGKDFPKSQKLSPDAFIQIALQLAYYRIYGQACATYESASLRMFHLGRTDTIRSASTDSLAFVKGMDDPKVPEQQRVELLRKAVQAHRAYTDRAIRGEAFDRHLLGLKLQAIEDLVSMPDIFMDTSYAIAMHFNLSTSQVPAKTDCVMSFGPVVPDGYGICYNPMEAHINFSVSAYNSCAETNAARMAHYLEKALLDMRTLLQNHPRAKL

Target ORF information:

RefSeq Version	XM_006233823.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus carnitine O-acetyltransferase (Crat), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006233823.2

ORF Insert Sequence:

ATGGAGGACG AGCAGCAGAG AGAGAAGGTG AAACCCCTGG GCCTCCTCAA GCCCTCCTCC 
TTGATGAAGG TGTCTGGTCG ATTTAAGGCC CACCAGGATG CACTGCCACG GCTGCCTGTG 
CCTCCACTTC AGCAATCCCT GGACCATTAC CTCAAGGCAC TGCAGCCCAT CGTGAGTGAG 
GAAGAATGGG CTCACACCAA GCAACTGGTG GACGAGTTTC AGACCTCAGG GGGTGTAGGG 
GAACGCCTGC AGAAGGGATT GGAGCGCAGG GCCAAGAAAA TGGAGAACTG GCTGTCCGAG 
TGGTGGCTCA AGACAGCCTA TCTCCAGTTC CGCCAGCCTG TGGTCATCTA CTCCAGTCCA 
GGAGTGCTGC TGCCCAAGCA GGACTTCATG GATCTACAGG GTCAGCTCCG GTTTGCTGCC 
AAACTCATCG AGGGTGTATT GGATTTCAAG TCCATGATTG ACAACGAGAC GCTGCCAGTT 
GAGTTCCTGG GAGGACAGCC ACTGTGCATG AACCAGTATT ATCAGATCCT GTCTTCTTGC 
CGTGTGCCTG GCCTCAAGCA GGACTCAGTG GTGAACTTCT TAAAGTCAAA GAAACCACCC 
ACACATATAA CCGTGGTGCA TAACTACCAG TTCTTCGAGC TGGATGTGTA CCACAGTGAT 
GGGACACCAC TCACCTCAGA TCAGATCTTT GTGCAGCTTG AGAAAATCTG GAACTCCTCA 
CTGCAATCCA ACAAGGAGCC TGTTGGCATC CTAACCTCCA ACCACCGGAA CAGCTGGGCC 
AAGGCCTACA GCAGCCTCAT TAAAGACAAA GTAAACCGGG AGTCGGTGAA CTCCATCCAG 
AAGAGCATCT TCACTGTGTG CCTGGACAAG CAAGTGCCCA GAGTCTCAGA TGATGTCTAC 
CGCAGCCACG TAGCCGGCCA GATGCTTCAT GGTGGTGGCA GCAAGTTCAA CAGTGGCAAC 
CGCTGGTTCG ACAAGACACT GCAGTTTATT GTGGCAGAAG ATGGCTCCTG TGGGATGGTT 
TATGAACATG CAGCTGCAGA AGGGCCCCCC ATTGTCGCTC TTGTGGACCA TGTCATGGAG 
TATACAAAGA AGCCTGAACT TGTGCGGTCC CCTATGGTAC CCCTGCCTAT GCCCAAGAAG 
CTGCGGTTCA ACATCACACC TGAGATCAAG AATGACATAG AGAAGGCCAA ACAGAACATC 
AGCATCATGA TCCAGGACCT GGACATCATG ATGCTGGTTT TCCATCACTT TGGAAAGGAC 
TTCCCCAAGT CCCAGAAGCT AAGCCCTGAT GCCTTCATCC AGATAGCCCT GCAGCTGGCA 
TACTACAGGA TCTATGGGCA GGCGTGTGCC ACGTATGAAA GCGCCTCTCT GCGCATGTTT 
CACCTGGGCC GCACTGACAC CATCCGCTCC GCCTCCACAG ACTCGCTGGC CTTTGTCAAA 
GGCATGGATG ACCCCAAAGT GCCGGAGCAG CAGAGGGTGG AGCTGCTTCG AAAGGCTGTG 
CAGGCCCACC GAGCCTACAC CGACCGCGCC ATCCGAGGGG AGGCCTTTGA CCGGCACTTG 
CTGGGTCTGA AGCTACAGGC CATCGAGGAC CTGGTGAGCA TGCCTGACAT CTTCATGGAT 
ACCTCCTACG CCATTGCCAT GCACTTCAAC CTTTCTACCA GCCAGGTCCC TGCCAAGACA 
GACTGTGTCA TGTCCTTCGG ACCTGTGGTC CCAGACGGTT ACGGCATCTG CTACAATCCC 
ATGGAGGCCC ATATCAACTT TTCCGTGTCA GCCTACAACA GCTGTGCTGA GACCAACGCT 
GCCCGCATGG CTCACTACTT GGAGAAAGCT CTGCTGGACA TGCGCACCTT ACTCCAGAAC 
CACCCCAGGG CCAAGCTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Obesity and lipid stress inhibit carnitine acetyltransferase activity.
Journal of lipid research55(4)635-44(2014 Apr)
Seiler SE,Martin OJ,Noland RC,Slentz DH,DeBalsi KL,Ilkayeva OR,An J,Newgard CB,Koves TR,Muoio DM

Chromatographic separation of reaction products from the choline acetyltransferase and carnitine acetyltransferase assay: differential ChAT and CrAT activity in brain extracts from Alzheimer's disease versus controls.
Journal of neurochemistry122(4)672-80(2012 Aug)
Bailey JA,Lahiri DK

Phosphoproteome analysis of functional mitochondria isolated from resting human muscle reveals extensive phosphorylation of inner membrane protein complexes and enzymes.
Molecular & cellular proteomics : MCP10(1)M110.000299(2011 Jan)
Zhao X,Le?n IR,Bak S,Mogensen M,Wrzesinski K,H?jlund K,Jensen ON

A proteome-wide perspective on peroxisome targeting signal 1(PTS1)-Pex5p affinities.
Journal of the American Chemical Society132(11)3973-9(2010 Mar)
Ghosh D,Berg JM

The carnitine acetyltransferase gene (CRAT): a characterization of porcine transcripts with insights into the 5'-end variants of mammalian transcripts and their possible sub-cellular localization.
Cellular & molecular biology letters14(1)90-9(2009)
Robic A,Faraut T,Liaubet L,Milan D

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Crat cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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