Myh7b cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Myh7b
Entrez Gene ID	311570
Full Name	myosin heavy chain 7B
General protein information	Preferred Names myosin heavy chain 7B Names myosin-7B myosin, heavy chain 7B, cardiac muscle, beta myosin, heavy polypeptide 7B, cardiac muscle, beta
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 3 Map Location: 3q42

mRNA and Protein(s)

mRNA	Protein	Name
XM_008762340.2	XP_008760562.1	myosin-7B isoform X1
XM_008762339.2	XP_008760561.1	myosin-7B isoform X1
XM_017591783.1	XP_017447272.1	myosin-7B isoform X2
NM_001107794.2	NP_001101264.2	myosin-7B
XM_017591782.1	XP_017447271.1	myosin-7B isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	MYH7B	XP_001160839.2
Gallus gallus (chicken)	MYH7B	NP_989918.1
Danio rerio (zebrafish)	myh7bb	XP_005162166.1
Caenorhabditis elegans (roundworm)	myo-3	NP_506065.2
Homo sapiens (human)	MYH7B	NP_065935.2
Bos taurus (cattle)	MYH7B	XP_003586892.2
Mus musculus (house mouse)	Myh7b	NP_001078847.1
Danio rerio (zebrafish)	myh7	XP_005162168.1
Caenorhabditis elegans (roundworm)	myo-2	NP_510092.2
Caenorhabditis elegans (roundworm)	myo-5	NP_505094.1
Rattus norvegicus (Norway rat)	Myh7b	NP_001101264.2
Caenorhabditis elegans (roundworm)	unc-54	NP_493596.1
Canis lupus familiaris (dog)	MYH7B	XP_534397.4
Caenorhabditis elegans (roundworm)	myo-1	NP_492053.1
Xenopus tropicalis (tropical clawed frog)	myh7b	XP_002932939.2

Gene Ontology
Bibliography

Related articles in PubMed

l-Arginine Attenuates Cardiac Dysfunction, But Further Down-Regulates ?-Myosin Heavy Chain Expression in Isoproterenol-Induced Cardiomyopathy.
Kralova E, Doka G, Pivackova L, Srankova J, Kuracinova K, Janega P, Babal P, Klimas J, Krenek P
Basic & clinical pharmacology & toxicology117(4)251-60(2015 Oct)

Developmental expression and cardiac transcriptional regulation of Myh7b, a third myosin heavy chain in the vertebrate heart.
Warkman AS, Whitman SA, Miller MK, Garriock RJ, Schwach CM, Gregorio CC, Krieg PA
Cytoskeleton (Hoboken, N.J.)69(5)324-35(2012 May)

Regulation of synapse structure and function by distinct myosin II motors.
Rubio MD, Johnson R, Miller CA, Huganir RL, Rumbaugh G
The Journal of neuroscience : the official journal of the Society for Neuroscience31(4)1448-60(2011 Jan)

Two novel/ancient myosins in mammalian skeletal muscles: MYH14/7b and MYH15 are expressed in extraocular muscles and muscle spindles.
Rossi AC, Mammucari C, Argentini C, Reggiani C, Schiaffino S
The Journal of physiology588(Pt 2)353-64(2010 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Myosin II motor activity is emerging as a broad regulatory mechanism for control over complex actin networks within dendritic spines.
Title: Regulation of synapse structure and function by distinct myosin II motors.

Myh7b, the ortholog of slow myosin 2 (SM2) of frogs and birds, is detected as mRNA in heart, slow muscles and extraocular muscles. MYH7b protein is detected only in a minor fiber population in extraocular muscles, corresponding to slow-tonic fibers.
Title: Two novel/ancient myosins in mammalian skeletal muscles: MYH14/7b and MYH15 are expressed in extraocular muscles and muscle spindles.

The following Myh7b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Myh7b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa00189	NM_001107794.2 Latest version!	Rattus norvegicus myosin heavy chain 7B (Myh7b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
ORa00189	XM_017591783.1 Latest version!	Rattus norvegicus myosin heavy chain 7B (Myh7b), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
ORa47041	XM_017591782.1 Latest version!	Rattus norvegicus myosin heavy chain 7B (Myh7b), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1091.30 $1559.00
ORa47041	XM_008762339.2 Latest version!	Rattus norvegicus myosin heavy chain 7B (Myh7b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1091.30 $1559.00
ORa47041	XM_008762340.2 Latest version!	Rattus norvegicus myosin heavy chain 7B (Myh7b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1091.30 $1559.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Myh7b ( NM_001107794.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001101264.2 Rattus norvegicus myosin heavy chain 7B (Myh7b), mRNA.

Price & Availability↑

CloneID	ORa00189
Clone ID Related Accession (Same CDS sequence)	XM_017591783.1 , NM_001107794.2
Accession Version	NM_001107794.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5826bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Rattus norvegicus(Norway rat)
Product	myosin-7B
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from EU241478.1. On Nov 5, 2008 this sequence version replaced NM_001107794.1. ##Evidence-Data-START## Transcript exon combination :: EU241478.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGATGGATA TGAATGAACT TGGAGAATCA GCCTGCTACC TCCGCCAGGG ATACCAGGAA 
ATGATGAAGG TGCACACTGT CCCATGGGAT GGCAAGAAAC GGGTCTGGGT GCCTGATGAA 
CAGGATGCCT ATGTCGAAGC TGAGGTCAAG ACTGAAGCTA CTGGAGGCCG AGTCACTGTG 
GAGACCAAAG ACCAGAAGGT GCTGACAGTG CGTGAAACGG AAATGCAGCC CATGAACCCA 
CCCCGCTTCG ACTTACTGGA GGACATGGCC ATGATGACGC ACCTCAACGA GGCTGCAGTG 
CTGCACAACC TGCGCCAGCG TTATGCTCGC TGGATGATCT ACACATACTC GGGCCTCTTC 
TGTGTCACCA TTAACCCATA CAAATGGCTC CCGGTCTACA CAGCTACTGT GGTGGCCGCC 
TACAAGGGCA AGCGCCGCTC GGAGGCCCCG CCTCATATAT ATGCAGTGGC AGATAATGCC 
TACAATGACA TGCTCCGCAA CAGAGAGAAC CAGTCCATGC TGATCACCGG AGAGTCGGGG 
GCCGGTAAGA CGGTTAACAC CAAGCGGGTC ATTCAGTATT TTGCCATCGT CGCTGCCCTG 
GGAGACGGGC CGGGCAAGAA GGCACAATTT CTGGCAACAA AAACCGGGGG CACCCTTGAG 
GATCAAATCA TCGAGGCTAA CCCTGCCATG GAGGCTTTTG GTAACGCCAA GACTCTGAGG 
AACGACAACT CCTCCCGTTT TGGCAAGTTC ATCCGCATCC ACTTTGGTCC CACTGGGAAG 
CTGGCCTCTG CGGACATTGA CAGCTATCTC CTGGAAAAGT CTCGAGTGAT CTTCCAGCTG 
CCCGGTGAGC GTGGTTACCA TGTCTACTAC CAAATCCTCT CCGGGAAGAA GCCAGAGCTG 
CAGGACATGC TGCTCCTCTC CATGAACCCC TACGACTACC ACTTCTGCAG TCAGGGGGTC 
ACCACCGTGG ATAACATGAA CGACGGGGAG GAGCTTATCG CCACTGATCA TGCAATGGAC 
ATCCTGGGCT TCAGCGTGGA TGAGAAGTGC GCATGCTATA AGATCGTGGG CGCCCTCCTG 
CACTTTGGCA ACATGAAGTT CAAACAGAAG CAGAGAGAGG AACAGGCTGA GGCAGACGGC 
ACTGAGAGTG CCGACAAGGC TGCCTACCTG ATGGGAGTCA GCAGTGGAGA CCTCCTCAAA 
GGTCTTCTGC ACCCCCGGGT TCGTGTGGGA AACGAGTATG TGACCAAGGG CCAGAGTGTG 
GAGCAGGTGG TATTTGCTGT GGGGGCCCTG GCCAAGGCTA CCTATGACCG GTTGTTCCGA 
TGGCTGGTAT CTCGAATCAA TCAGACCCTG GACACAAAGC TCCCCCGTCA GTTCTTCATT 
GGGGTCCTGG ATATCGCTGG TTTTGAGATC TTTGAGTTTA ACAGCTTCGA GCAACTGTGT 
ATCAACTTCA CCAACGAGAA ACTGCAGCAG TTCTTCAACC AGCACATGTT CGTGCTGGAG 
CAGGAGGAGT ACAAACGGGA GGGCATTGAC TGGGTCTTCA TCGACTTCGG CCTTGACCTG 
CAGCCCTGCA TCGACCTCAT CGAGAAGCCT CTGGGCATTC TGTCCATCCT GGAGGAGGAG 
TGCATGTTCC CCAAGGCTTC AGACGCAAGC TTCAGGGCCA AGCTCTATGA CAATCACTCT 
GGGAAGTCAC CTAATTTCCA GCAGCCTCGC CCAGACAAGA AACGCAAGTA CCAGGCTCAC 
TTCGAGGTGG TCCACTACGC AGGCGTGGTA CCTTATAGCA TTGTGGGCTG GCTGGAGAAA 
AACAAGGACC CCCTCAATGA GACCGTGGTC CCCATCTTCC AAAAGTCACA GAATAGGCTC 
TTGGCCACCC TCTATGAGAA CTATGCAGGT TCTTGCTCCA CCGAGCCCCC CAAGTCTGGG 
GTGAAAGAGA AACGAAAGAA AGCAGCATCA TTTCAGACAG TGTCTCAGCT TCACAAGGAG 
AACCTCAACA AGCTTATGAC CAACCTGCGG GCCACACAGC CCCACTTCGT CCGTTGTATC 
GTCCCCAACG AGAACAAGAC TCCAGGGGTC ATGGATTCCT TCTTGGTGCT ACACCAGCTA 
CGCTGTAACG GGGTCCTAGA GGGGATCCGG ATCTGCCGCC AAGGGTTCCC CAACAGACTG 
CTGTATGCTG ACTTCAGGCA GCGGTACCGA ATCCTGAACC CCAGTGCCAT CCCAGATGAC 
ACCTTTGTGG ACAGCAGGAA GGCCACAGAG AAGCTGCTGG GCTCTCTGGA CATTGACCAC 
ACCCAGTATC AGTTTGGCCA CACCAAGGTG TTCTTCAAGG CTGGGCTTCT AGGCATCCTG 
GAGGAGCTCC GGGACCAGCG TCTGGCTAAG GTGCTGACAC TGCTGCAGGC TCGGAGCCGG 
GGCCGCCTCA TGCGTCTGGA GTACCAGCGC ATGCTAGGAG GAAGGGATGC CCTGTTCACC 
ATCCAGTGGA ACATCCGGGC CTTCAATGCC GTCAAGAACT GGTCCTGGAT GAAGCTCTTC 
TTCAAGATGA AGCCACTGCT GCGCTCAGCA CAGGCAGAGG AAGAGCTGGC AGCACTTCGG 
GCAGAACTGC GGGGGCTGCG GGGGGCCCTG GCTACGGCTG AGGCCAAGCG TCAGGAGCTG 
GAGGAGACTC AAGTCAGTGT CACTCAGGAG AAGAATGACC TGGCCCTGCA GCTGCAGGCA 
GAGCAGGACA ACCTAGCGGA TGCGGAGGAG CGCTGCCACT TGCTCATCAA GTCCAAGGTG 
CAGCTCGAGG CGAAGGTGAA GGAGCTGAAC GAGCGGCTGG AGGATGAGGA GGAAGTGAAC 
GCTGACCTGG CCGCACGCAG GCGCAAGCTG GAGGATGAGT GCACGGAGCT CAAGAAGGAC 
ATTGATGACC TGGAGCTGAC CCTGGCCAAG GCTGAGAAGG AGAAACAAGC CACCGAGAAT 
AAGGTGAAGA ACCTGACAGA GGAGATGGCT GCCCTGGACG AGTCCGTGGT CCGCCTGACT 
AAGGAGAAGA AGGCGTTGCA GGAGGCTCAC CAGCAGGCTC TGGGTGACCT GCAGGCTGAA 
GAGGACCGTG TGAGTGCGCT GGCCAAGGCC AAGATCCGGT TGGAGCAGCA AGTAGAGGAC 
CTGGAGTGCT CCCTGGAGCA AGAGAAGAAG CTTCGCATGG ATACAGAACG TGCCAAGCGC 
AAGCTCGAAG GAGACCTGAA GCTGACGCAG GAGACAGTGA CAGACACCAC ACAAGACAAG 
CAGCAGCTGG AGGAGAAGTT GAAGAAGAAG GATTCAGAGC TGAGTCAGCT GAGCCTGCGG 
GTGGAGGATG AGCAGCTCCT GGGGGCGCAG TTGCAGAAGA AGATCAAGGA GCTACAGGCG 
AGGGCTGAGG AGCTGGAGGA GGAGCTGGAG GCGGAGCGAG CAGCCCGTGC CCGTGTGGAA 
AAGCAGCGAG CTGAGGCAGC CCGAGAGCTG GAGGAGCTGA GTGAGAGGCT AGAGGAGGCT 
GGTGGAGCCT CTGCCGGGCA GCGTGAGGGT TGCCGCAAGC GTGAGGCTGA GTTGGGCCGA 
CTGCGGCGGG AGCTGGAGGA GACAGTTCTG AGACACGAGG CCACTGTGGC TGCCCTGCGC 
CGCAAGCAGG CTGATAGCGC CGCAGAGTTG AGCGAGCAGG TAGACAGCCT CCAGCGGATC 
CGGCAGAAAC TGGAGAAGGA AAAGAGTGAG CTGCGCATGG AGGTGGATGA TCTGGGGGCC 
AGCGTGGAAA CTCTGGCCCG TGGCAAGGCC AGTGCAGAGA AGCTGTGCCG GACCTATGAG 
GACCAGCTCA GCGAGGCCAA GATCAAAGTG GAGGAGCTGC AGCGGCAGCT GACTGACGCC 
AGCACCCAGC GGGGGCGGTT ACAAACGGAG AACGGGGAGC TGGGCCGCCT GCTTGAGGAG 
AAGGAGTCTA TGATCAGCCA GCTGAGCCGC GGGAAGACCT CAGCCGCTCA GAGTCTGGAG 
GAACTTCGGC GGCAGTTGGA GGAAGAAAGC AAGGCCAAGG GTGCGCTGGC TCACGCAGTG 
CAGGCTCTGC GGCACGACTG CGACCTCCTG CGAGAGCAGC ATGAGGAAGA GTCCGAGGCG 
CAGGCTGAGC TGCAGCGGCT GCTGTCCAAA GCCAACGCTG AGGTGGCACA GTGGAGGAGC 
AAGTATGAGG CGGACGCCAT CCAAAGGACG GAGGAGCTGG AGGAGGCCAA GAAGAAGCTG 
GCCCTGAGGC TGCAGGAGGC AGAAGAAGGC GTAGAGGCTG CGAATGCCAA GTGCTCCTCA 
CTGGAGAAGG CCAAGCTGCG GCTGCAGACA GAGTCCGAGG ACGTGACCTT GGAGCTGGAG 
CGGGCCACCT CAGCCGCTGC TGCACTGGAT AAGAAGCAGC GGCACTTGGA GCGGGCCCTG 
GAGGAACGGA GGCGGCAGGA GGAGGAGATG CAGCGGGAAC TGGAGGCGGC GCAGAGGGAG 
GCTCGAGGTC TGGGCACCGA GCTCTTTCGA TTGCGGCACA GCCATGAGGA GGCGCTTGAG 
GCTCTGGAGA CACTCAAGCG GGAGAACAAG AATCTGCAGG AGGAGATCAG CGACCTTACA 
GACCAGGTCA GCCTCAGCGG GAAGAGCATC CAGGAACTGG AGAAGGCCAA GAAGGCGCTG 
GAAGGGGAGA AGAGTGAGCT GCAGGCTGCG CTGGAGGAGG CTGAGGGGGC GCTGGAGCTG 
GAAGAGACAA AGACCCTGCG GATCCAGTTG GAGTTGTCCC AGGTCAAGGC GGAAGTGGAC 
CGAAAGCTGG CGGAGAAGGA TGAGGAGTGT ACTAACCTGA GGCGCAACCA CCAGCGGGCT 
GTGGAGTCCT TGCAGGCCTC CCTGGATGCC GAGACTAGGG CCCGCAATGA AGCACTGCGT 
CTCAAGAAGA AGATGGAGGG GGACCTCAAT GACCTGGAGT TACAGCTGGG CCACGCAACC 
CGCCAGGCCA TGGAGGCACA GGCAGCCACG AGGCTGCTTC AGGCCCAACT CAAGGAGGAG 
CAAGCAGGGC GTGATGAGGA GCAGAGGCTG GCTGCCGAGC TCCGCGAGCA GGGCCAGGCC 
CTCGAGCGCA GGGCTGCACT GCTGGCATCT GAGCTGGAAG AGCTTCGGGC CGCCCTGGAA 
CAGGGCGAGC GCAGCCGAAG GCTGGCTGAA CAGGAGCTGT TGGAGGCCAC AGAGCGCCTC 
AACCTACTGC ATTCACAGAA CACAGGCCTC CTGAACCAGA AAAAGAAGCT GGAGGTGGAC 
TTGGCCCAGC TGAGTGGGGA GGTGGAGGAG GCTGCGCAGG AGAGGCGGGA AGCTGAGGAG 
AAGGCCAAAA AGGCCATCAC GGATGCAGCC ATGATGGCTG AGGAGCTGAA AAAGGAGCAG 
GACACAAGTG CACACCTCGA ACGGATGAAG AAGACCCTGG AACAGACAGT GCGGGAGCTG 
CAGGCCCGCC TTGAGGAAGC AGAGCAGGCC GCACTCCGGG GTGGGAAGAA GCAAGTGCAG 
AAGCTGGAAG CCAAGGTGCG GGAACTGGAG GCAGAGCTTG ACGCAGAGCA GAAGAAGCAC 
GCTGAGGCCC TCAAGGGTGT GCGCAAACAT GAGCGCAGGG TCAAGGAGCT TGCGTACCAG 
ACTGAGGAAG ACAGGAAGAA CCTGGCTCGC ATGCAGGACC TAGTGGACAA GCTGCAGAGC 
AAGGTCAAGA GCTACAAGCG TCAGTTTGAG GAGGCGGAGC AGCAGGCCAG TACCAACCTG 
GCTAAGTACC GCAAGGCCCA GCACGAGTTA GACGACGCTG AGGAGCGGGC AGACATGGCA 
GAGACCCAGG CCAACAAGCT GCGGGCAAGG TCCAGGGATG CCCTGGGTCC CAAGCACAAG 
GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001101264.2
CDS	136..5961
Translation	MMDMNELGESACYLRQGYQEMMKVHTVPWDGKKRVWVPDEQDAYVEAEVKTEATGGRVTVETKDQKVLTVRETEMQPMNPPRFDLLEDMAMMTHLNEAAVLHNLRQRYARWMIYTYSGLFCVTINPYKWLPVYTATVVAAYKGKRRSEAPPHIYAVADNAYNDMLRNRENQSMLITGESGAGKTVNTKRVIQYFAIVAALGDGPGKKAQFLATKTGGTLEDQIIEANPAMEAFGNAKTLRNDNSSRFGKFIRIHFGPTGKLASADIDSYLLEKSRVIFQLPGERGYHVYYQILSGKKPELQDMLLLSMNPYDYHFCSQGVTTVDNMNDGEELIATDHAMDILGFSVDEKCACYKIVGALLHFGNMKFKQKQREEQAEADGTESADKAAYLMGVSSGDLLKGLLHPRVRVGNEYVTKGQSVEQVVFAVGALAKATYDRLFRWLVSRINQTLDTKLPRQFFIGVLDIAGFEIFEFNSFEQLCINFTNEKLQQFFNQHMFVLEQEEYKREGIDWVFIDFGLDLQPCIDLIEKPLGILSILEEECMFPKASDASFRAKLYDNHSGKSPNFQQPRPDKKRKYQAHFEVVHYAGVVPYSIVGWLEKNKDPLNETVVPIFQKSQNRLLATLYENYAGSCSTEPPKSGVKEKRKKAASFQTVSQLHKENLNKLMTNLRATQPHFVRCIVPNENKTPGVMDSFLVLHQLRCNGVLEGIRICRQGFPNRLLYADFRQRYRILNPSAIPDDTFVDSRKATEKLLGSLDIDHTQYQFGHTKVFFKAGLLGILEELRDQRLAKVLTLLQARSRGRLMRLEYQRMLGGRDALFTIQWNIRAFNAVKNWSWMKLFFKMKPLLRSAQAEEELAALRAELRGLRGALATAEAKRQELEETQVSVTQEKNDLALQLQAEQDNLADAEERCHLLIKSKVQLEAKVKELNERLEDEEEVNADLAARRRKLEDECTELKKDIDDLELTLAKAEKEKQATENKVKNLTEEMAALDESVVRLTKEKKALQEAHQQALGDLQAEEDRVSALAKAKIRLEQQVEDLECSLEQEKKLRMDTERAKRKLEGDLKLTQETVTDTTQDKQQLEEKLKKKDSELSQLSLRVEDEQLLGAQLQKKIKELQARAEELEEELEAERAARARVEKQRAEAARELEELSERLEEAGGASAGQREGCRKREAELGRLRRELEETVLRHEATVAALRRKQADSAAELSEQVDSLQRIRQKLEKEKSELRMEVDDLGASVETLARGKASAEKLCRTYEDQLSEAKIKVEELQRQLTDASTQRGRLQTENGELGRLLEEKESMISQLSRGKTSAAQSLEELRRQLEEESKAKGALAHAVQALRHDCDLLREQHEEESEAQAELQRLLSKANAEVAQWRSKYEADAIQRTEELEEAKKKLALRLQEAEEGVEAANAKCSSLEKAKLRLQTESEDVTLELERATSAAAALDKKQRHLERALEERRRQEEEMQRELEAAQREARGLGTELFRLRHSHEEALEALETLKRENKNLQEEISDLTDQVSLSGKSIQELEKAKKALEGEKSELQAALEEAEGALELEETKTLRIQLELSQVKAEVDRKLAEKDEECTNLRRNHQRAVESLQASLDAETRARNEALRLKKKMEGDLNDLELQLGHATRQAMEAQAATRLLQAQLKEEQAGRDEEQRLAAELREQGQALERRAALLASELEELRAALEQGERSRRLAEQELLEATERLNLLHSQNTGLLNQKKKLEVDLAQLSGEVEEAAQERREAEEKAKKAITDAAMMAEELKKEQDTSAHLERMKKTLEQTVRELQARLEEAEQAALRGGKKQVQKLEAKVRELEAELDAEQKKHAEALKGVRKHERRVKELAYQTEEDRKNLARMQDLVDKLQSKVKSYKRQFEEAEQQASTNLAKYRKAQHELDDAEERADMAETQANKLRARSRDALGPKHKE

Target ORF information:

RefSeq Version	NM_001107794.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus myosin heavy chain 7B (Myh7b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001107794.2

ORF Insert Sequence:

ATGATGGATA TGAATGAACT TGGAGAATCA GCCTGCTACC TCCGCCAGGG ATACCAGGAA 
ATGATGAAGG TGCACACTGT CCCATGGGAT GGCAAGAAAC GGGTCTGGGT GCCTGATGAA 
CAGGATGCCT ATGTCGAAGC TGAGGTCAAG ACTGAAGCTA CTGGAGGCCG AGTCACTGTG 
GAGACCAAAG ACCAGAAGGT GCTGACAGTG CGTGAAACGG AAATGCAGCC CATGAACCCA 
CCCCGCTTCG ACTTACTGGA GGACATGGCC ATGATGACGC ACCTCAACGA GGCTGCAGTG 
CTGCACAACC TGCGCCAGCG TTATGCTCGC TGGATGATCT ACACATACTC GGGCCTCTTC 
TGTGTCACCA TTAACCCATA CAAATGGCTC CCGGTCTACA CAGCTACTGT GGTGGCCGCC 
TACAAGGGCA AGCGCCGCTC GGAGGCCCCG CCTCATATAT ATGCAGTGGC AGATAATGCC 
TACAATGACA TGCTCCGCAA CAGAGAGAAC CAGTCCATGC TGATCACCGG AGAGTCGGGG 
GCCGGTAAGA CGGTTAACAC CAAGCGGGTC ATTCAGTATT TTGCCATCGT CGCTGCCCTG 
GGAGACGGGC CGGGCAAGAA GGCACAATTT CTGGCAACAA AAACCGGGGG CACCCTTGAG 
GATCAAATCA TCGAGGCTAA CCCTGCCATG GAGGCTTTTG GTAACGCCAA GACTCTGAGG 
AACGACAACT CCTCCCGTTT TGGCAAGTTC ATCCGCATCC ACTTTGGTCC CACTGGGAAG 
CTGGCCTCTG CGGACATTGA CAGCTATCTC CTGGAAAAGT CTCGAGTGAT CTTCCAGCTG 
CCCGGTGAGC GTGGTTACCA TGTCTACTAC CAAATCCTCT CCGGGAAGAA GCCAGAGCTG 
CAGGACATGC TGCTCCTCTC CATGAACCCC TACGACTACC ACTTCTGCAG TCAGGGGGTC 
ACCACCGTGG ATAACATGAA CGACGGGGAG GAGCTTATCG CCACTGATCA TGCAATGGAC 
ATCCTGGGCT TCAGCGTGGA TGAGAAGTGC GCATGCTATA AGATCGTGGG CGCCCTCCTG 
CACTTTGGCA ACATGAAGTT CAAACAGAAG CAGAGAGAGG AACAGGCTGA GGCAGACGGC 
ACTGAGAGTG CCGACAAGGC TGCCTACCTG ATGGGAGTCA GCAGTGGAGA CCTCCTCAAA 
GGTCTTCTGC ACCCCCGGGT TCGTGTGGGA AACGAGTATG TGACCAAGGG CCAGAGTGTG 
GAGCAGGTGG TATTTGCTGT GGGGGCCCTG GCCAAGGCTA CCTATGACCG GTTGTTCCGA 
TGGCTGGTAT CTCGAATCAA TCAGACCCTG GACACAAAGC TCCCCCGTCA GTTCTTCATT 
GGGGTCCTGG ATATCGCTGG TTTTGAGATC TTTGAGTTTA ACAGCTTCGA GCAACTGTGT 
ATCAACTTCA CCAACGAGAA ACTGCAGCAG TTCTTCAACC AGCACATGTT CGTGCTGGAG 
CAGGAGGAGT ACAAACGGGA GGGCATTGAC TGGGTCTTCA TCGACTTCGG CCTTGACCTG 
CAGCCCTGCA TCGACCTCAT CGAGAAGCCT CTGGGCATTC TGTCCATCCT GGAGGAGGAG 
TGCATGTTCC CCAAGGCTTC AGACGCAAGC TTCAGGGCCA AGCTCTATGA CAATCACTCT 
GGGAAGTCAC CTAATTTCCA GCAGCCTCGC CCAGACAAGA AACGCAAGTA CCAGGCTCAC 
TTCGAGGTGG TCCACTACGC AGGCGTGGTA CCTTATAGCA TTGTGGGCTG GCTGGAGAAA 
AACAAGGACC CCCTCAATGA GACCGTGGTC CCCATCTTCC AAAAGTCACA GAATAGGCTC 
TTGGCCACCC TCTATGAGAA CTATGCAGGT TCTTGCTCCA CCGAGCCCCC CAAGTCTGGG 
GTGAAAGAGA AACGAAAGAA AGCAGCATCA TTTCAGACAG TGTCTCAGCT TCACAAGGAG 
AACCTCAACA AGCTTATGAC CAACCTGCGG GCCACACAGC CCCACTTCGT CCGTTGTATC 
GTCCCCAACG AGAACAAGAC TCCAGGGGTC ATGGATTCCT TCTTGGTGCT ACACCAGCTA 
CGCTGTAACG GGGTCCTAGA GGGGATCCGG ATCTGCCGCC AAGGGTTCCC CAACAGACTG 
CTGTATGCTG ACTTCAGGCA GCGGTACCGA ATCCTGAACC CCAGTGCCAT CCCAGATGAC 
ACCTTTGTGG ACAGCAGGAA GGCCACAGAG AAGCTGCTGG GCTCTCTGGA CATTGACCAC 
ACCCAGTATC AGTTTGGCCA CACCAAGGTG TTCTTCAAGG CTGGGCTTCT AGGCATCCTG 
GAGGAGCTCC GGGACCAGCG TCTGGCTAAG GTGCTGACAC TGCTGCAGGC TCGGAGCCGG 
GGCCGCCTCA TGCGTCTGGA GTACCAGCGC ATGCTAGGAG GAAGGGATGC CCTGTTCACC 
ATCCAGTGGA ACATCCGGGC CTTCAATGCC GTCAAGAACT GGTCCTGGAT GAAGCTCTTC 
TTCAAGATGA AGCCACTGCT GCGCTCAGCA CAGGCAGAGG AAGAGCTGGC AGCACTTCGG 
GCAGAACTGC GGGGGCTGCG GGGGGCCCTG GCTACGGCTG AGGCCAAGCG TCAGGAGCTG 
GAGGAGACTC AAGTCAGTGT CACTCAGGAG AAGAATGACC TGGCCCTGCA GCTGCAGGCA 
GAGCAGGACA ACCTAGCGGA TGCGGAGGAG CGCTGCCACT TGCTCATCAA GTCCAAGGTG 
CAGCTCGAGG CGAAGGTGAA GGAGCTGAAC GAGCGGCTGG AGGATGAGGA GGAAGTGAAC 
GCTGACCTGG CCGCACGCAG GCGCAAGCTG GAGGATGAGT GCACGGAGCT CAAGAAGGAC 
ATTGATGACC TGGAGCTGAC CCTGGCCAAG GCTGAGAAGG AGAAACAAGC CACCGAGAAT 
AAGGTGAAGA ACCTGACAGA GGAGATGGCT GCCCTGGACG AGTCCGTGGT CCGCCTGACT 
AAGGAGAAGA AGGCGTTGCA GGAGGCTCAC CAGCAGGCTC TGGGTGACCT GCAGGCTGAA 
GAGGACCGTG TGAGTGCGCT GGCCAAGGCC AAGATCCGGT TGGAGCAGCA AGTAGAGGAC 
CTGGAGTGCT CCCTGGAGCA AGAGAAGAAG CTTCGCATGG ATACAGAACG TGCCAAGCGC 
AAGCTCGAAG GAGACCTGAA GCTGACGCAG GAGACAGTGA CAGACACCAC ACAAGACAAG 
CAGCAGCTGG AGGAGAAGTT GAAGAAGAAG GATTCAGAGC TGAGTCAGCT GAGCCTGCGG 
GTGGAGGATG AGCAGCTCCT GGGGGCGCAG TTGCAGAAGA AGATCAAGGA GCTACAGGCG 
AGGGCTGAGG AGCTGGAGGA GGAGCTGGAG GCGGAGCGAG CAGCCCGTGC CCGTGTGGAA 
AAGCAGCGAG CTGAGGCAGC CCGAGAGCTG GAGGAGCTGA GTGAGAGGCT AGAGGAGGCT 
GGTGGAGCCT CTGCCGGGCA GCGTGAGGGT TGCCGCAAGC GTGAGGCTGA GTTGGGCCGA 
CTGCGGCGGG AGCTGGAGGA GACAGTTCTG AGACACGAGG CCACTGTGGC TGCCCTGCGC 
CGCAAGCAGG CTGATAGCGC CGCAGAGTTG AGCGAGCAGG TAGACAGCCT CCAGCGGATC 
CGGCAGAAAC TGGAGAAGGA AAAGAGTGAG CTGCGCATGG AGGTGGATGA TCTGGGGGCC 
AGCGTGGAAA CTCTGGCCCG TGGCAAGGCC AGTGCAGAGA AGCTGTGCCG GACCTATGAG 
GACCAGCTCA GCGAGGCCAA GATCAAAGTG GAGGAGCTGC AGCGGCAGCT GACTGACGCC 
AGCACCCAGC GGGGGCGGTT ACAAACGGAG AACGGGGAGC TGGGCCGCCT GCTTGAGGAG 
AAGGAGTCTA TGATCAGCCA GCTGAGCCGC GGGAAGACCT CAGCCGCTCA GAGTCTGGAG 
GAACTTCGGC GGCAGTTGGA GGAAGAAAGC AAGGCCAAGG GTGCGCTGGC TCACGCAGTG 
CAGGCTCTGC GGCACGACTG CGACCTCCTG CGAGAGCAGC ATGAGGAAGA GTCCGAGGCG 
CAGGCTGAGC TGCAGCGGCT GCTGTCCAAA GCCAACGCTG AGGTGGCACA GTGGAGGAGC 
AAGTATGAGG CGGACGCCAT CCAAAGGACG GAGGAGCTGG AGGAGGCCAA GAAGAAGCTG 
GCCCTGAGGC TGCAGGAGGC AGAAGAAGGC GTAGAGGCTG CGAATGCCAA GTGCTCCTCA 
CTGGAGAAGG CCAAGCTGCG GCTGCAGACA GAGTCCGAGG ACGTGACCTT GGAGCTGGAG 
CGGGCCACCT CAGCCGCTGC TGCACTGGAT AAGAAGCAGC GGCACTTGGA GCGGGCCCTG 
GAGGAACGGA GGCGGCAGGA GGAGGAGATG CAGCGGGAAC TGGAGGCGGC GCAGAGGGAG 
GCTCGAGGTC TGGGCACCGA GCTCTTTCGA TTGCGGCACA GCCATGAGGA GGCGCTTGAG 
GCTCTGGAGA CACTCAAGCG GGAGAACAAG AATCTGCAGG AGGAGATCAG CGACCTTACA 
GACCAGGTCA GCCTCAGCGG GAAGAGCATC CAGGAACTGG AGAAGGCCAA GAAGGCGCTG 
GAAGGGGAGA AGAGTGAGCT GCAGGCTGCG CTGGAGGAGG CTGAGGGGGC GCTGGAGCTG 
GAAGAGACAA AGACCCTGCG GATCCAGTTG GAGTTGTCCC AGGTCAAGGC GGAAGTGGAC 
CGAAAGCTGG CGGAGAAGGA TGAGGAGTGT ACTAACCTGA GGCGCAACCA CCAGCGGGCT 
GTGGAGTCCT TGCAGGCCTC CCTGGATGCC GAGACTAGGG CCCGCAATGA AGCACTGCGT 
CTCAAGAAGA AGATGGAGGG GGACCTCAAT GACCTGGAGT TACAGCTGGG CCACGCAACC 
CGCCAGGCCA TGGAGGCACA GGCAGCCACG AGGCTGCTTC AGGCCCAACT CAAGGAGGAG 
CAAGCAGGGC GTGATGAGGA GCAGAGGCTG GCTGCCGAGC TCCGCGAGCA GGGCCAGGCC 
CTCGAGCGCA GGGCTGCACT GCTGGCATCT GAGCTGGAAG AGCTTCGGGC CGCCCTGGAA 
CAGGGCGAGC GCAGCCGAAG GCTGGCTGAA CAGGAGCTGT TGGAGGCCAC AGAGCGCCTC 
AACCTACTGC ATTCACAGAA CACAGGCCTC CTGAACCAGA AAAAGAAGCT GGAGGTGGAC 
TTGGCCCAGC TGAGTGGGGA GGTGGAGGAG GCTGCGCAGG AGAGGCGGGA AGCTGAGGAG 
AAGGCCAAAA AGGCCATCAC GGATGCAGCC ATGATGGCTG AGGAGCTGAA AAAGGAGCAG 
GACACAAGTG CACACCTCGA ACGGATGAAG AAGACCCTGG AACAGACAGT GCGGGAGCTG 
CAGGCCCGCC TTGAGGAAGC AGAGCAGGCC GCACTCCGGG GTGGGAAGAA GCAAGTGCAG 
AAGCTGGAAG CCAAGGTGCG GGAACTGGAG GCAGAGCTTG ACGCAGAGCA GAAGAAGCAC 
GCTGAGGCCC TCAAGGGTGT GCGCAAACAT GAGCGCAGGG TCAAGGAGCT TGCGTACCAG 
ACTGAGGAAG ACAGGAAGAA CCTGGCTCGC ATGCAGGACC TAGTGGACAA GCTGCAGAGC 
AAGGTCAAGA GCTACAAGCG TCAGTTTGAG GAGGCGGAGC AGCAGGCCAG TACCAACCTG 
GCTAAGTACC GCAAGGCCCA GCACGAGTTA GACGACGCTG AGGAGCGGGC AGACATGGCA 
GAGACCCAGG CCAACAAGCT GCGGGCAAGG TCCAGGGATG CCCTGGGTCC CAAGCACAAG 
GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Myh7b ( XM_017591783.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017447272.1 Rattus norvegicus myosin heavy chain 7B (Myh7b), transcript variant X4, mRNA.

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CloneID	ORa00189
Clone ID Related Accession (Same CDS sequence)	XM_017591783.1 , NM_001107794.2
Accession Version	XM_017591783.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5826bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	myosin-7B isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005102.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGATA TGAATGAACT TGGAGAATCA GCCTGCTACC TCCGCCAGGG ATACCAGGAA 
ATGATGAAGG TGCACACTGT CCCATGGGAT GGCAAGAAAC GGGTCTGGGT GCCTGATGAA 
CAGGATGCCT ATGTCGAAGC TGAGGTCAAG ACTGAAGCTA CTGGAGGCCG AGTCACTGTG 
GAGACCAAAG ACCAGAAGGT GCTGACAGTG CGTGAAACGG AAATGCAGCC CATGAACCCA 
CCCCGCTTCG ACTTACTGGA GGACATGGCC ATGATGACGC ACCTCAACGA GGCTGCAGTG 
CTGCACAACC TGCGCCAGCG TTATGCTCGC TGGATGATCT ACACATACTC GGGCCTCTTC 
TGTGTCACCA TTAACCCATA CAAATGGCTC CCGGTCTACA CAGCTACTGT GGTGGCCGCC 
TACAAGGGCA AGCGCCGCTC GGAGGCCCCG CCTCATATAT ATGCAGTGGC AGATAATGCC 
TACAATGACA TGCTCCGCAA CAGAGAGAAC CAGTCCATGC TGATCACCGG AGAGTCGGGG 
GCCGGTAAGA CGGTTAACAC CAAGCGGGTC ATTCAGTATT TTGCCATCGT CGCTGCCCTG 
GGAGACGGGC CGGGCAAGAA GGCACAATTT CTGGCAACAA AAACCGGGGG CACCCTTGAG 
GATCAAATCA TCGAGGCTAA CCCTGCCATG GAGGCTTTTG GTAACGCCAA GACTCTGAGG 
AACGACAACT CCTCCCGTTT TGGCAAGTTC ATCCGCATCC ACTTTGGTCC CACTGGGAAG 
CTGGCCTCTG CGGACATTGA CAGCTATCTC CTGGAAAAGT CTCGAGTGAT CTTCCAGCTG 
CCCGGTGAGC GTGGTTACCA TGTCTACTAC CAAATCCTCT CCGGGAAGAA GCCAGAGCTG 
CAGGACATGC TGCTCCTCTC CATGAACCCC TACGACTACC ACTTCTGCAG TCAGGGGGTC 
ACCACCGTGG ATAACATGAA CGACGGGGAG GAGCTTATCG CCACTGATCA TGCAATGGAC 
ATCCTGGGCT TCAGCGTGGA TGAGAAGTGC GCATGCTATA AGATCGTGGG CGCCCTCCTG 
CACTTTGGCA ACATGAAGTT CAAACAGAAG CAGAGAGAGG AACAGGCTGA GGCAGACGGC 
ACTGAGAGTG CCGACAAGGC TGCCTACCTG ATGGGAGTCA GCAGTGGAGA CCTCCTCAAA 
GGTCTTCTGC ACCCCCGGGT TCGTGTGGGA AACGAGTATG TGACCAAGGG CCAGAGTGTG 
GAGCAGGTGG TATTTGCTGT GGGGGCCCTG GCCAAGGCTA CCTATGACCG GTTGTTCCGA 
TGGCTGGTAT CTCGAATCAA TCAGACCCTG GACACAAAGC TCCCCCGTCA GTTCTTCATT 
GGGGTCCTGG ATATCGCTGG TTTTGAGATC TTTGAGTTTA ACAGCTTCGA GCAACTGTGT 
ATCAACTTCA CCAACGAGAA ACTGCAGCAG TTCTTCAACC AGCACATGTT CGTGCTGGAG 
CAGGAGGAGT ACAAACGGGA GGGCATTGAC TGGGTCTTCA TCGACTTCGG CCTTGACCTG 
CAGCCCTGCA TCGACCTCAT CGAGAAGCCT CTGGGCATTC TGTCCATCCT GGAGGAGGAG 
TGCATGTTCC CCAAGGCTTC AGACGCAAGC TTCAGGGCCA AGCTCTATGA CAATCACTCT 
GGGAAGTCAC CTAATTTCCA GCAGCCTCGC CCAGACAAGA AACGCAAGTA CCAGGCTCAC 
TTCGAGGTGG TCCACTACGC AGGCGTGGTA CCTTATAGCA TTGTGGGCTG GCTGGAGAAA 
AACAAGGACC CCCTCAATGA GACCGTGGTC CCCATCTTCC AAAAGTCACA GAATAGGCTC 
TTGGCCACCC TCTATGAGAA CTATGCAGGT TCTTGCTCCA CCGAGCCCCC CAAGTCTGGG 
GTGAAAGAGA AACGAAAGAA AGCAGCATCA TTTCAGACAG TGTCTCAGCT TCACAAGGAG 
AACCTCAACA AGCTTATGAC CAACCTGCGG GCCACACAGC CCCACTTCGT CCGTTGTATC 
GTCCCCAACG AGAACAAGAC TCCAGGGGTC ATGGATTCCT TCTTGGTGCT ACACCAGCTA 
CGCTGTAACG GGGTCCTAGA GGGGATCCGG ATCTGCCGCC AAGGGTTCCC CAACAGACTG 
CTGTATGCTG ACTTCAGGCA GCGGTACCGA ATCCTGAACC CCAGTGCCAT CCCAGATGAC 
ACCTTTGTGG ACAGCAGGAA GGCCACAGAG AAGCTGCTGG GCTCTCTGGA CATTGACCAC 
ACCCAGTATC AGTTTGGCCA CACCAAGGTG TTCTTCAAGG CTGGGCTTCT AGGCATCCTG 
GAGGAGCTCC GGGACCAGCG TCTGGCTAAG GTGCTGACAC TGCTGCAGGC TCGGAGCCGG 
GGCCGCCTCA TGCGTCTGGA GTACCAGCGC ATGCTAGGAG GAAGGGATGC CCTGTTCACC 
ATCCAGTGGA ACATCCGGGC CTTCAATGCC GTCAAGAACT GGTCCTGGAT GAAGCTCTTC 
TTCAAGATGA AGCCACTGCT GCGCTCAGCA CAGGCAGAGG AAGAGCTGGC AGCACTTCGG 
GCAGAACTGC GGGGGCTGCG GGGGGCCCTG GCTACGGCTG AGGCCAAGCG TCAGGAGCTG 
GAGGAGACTC AAGTCAGTGT CACTCAGGAG AAGAATGACC TGGCCCTGCA GCTGCAGGCA 
GAGCAGGACA ACCTAGCGGA TGCGGAGGAG CGCTGCCACT TGCTCATCAA GTCCAAGGTG 
CAGCTCGAGG CGAAGGTGAA GGAGCTGAAC GAGCGGCTGG AGGATGAGGA GGAAGTGAAC 
GCTGACCTGG CCGCACGCAG GCGCAAGCTG GAGGATGAGT GCACGGAGCT CAAGAAGGAC 
ATTGATGACC TGGAGCTGAC CCTGGCCAAG GCTGAGAAGG AGAAACAAGC CACCGAGAAT 
AAGGTGAAGA ACCTGACAGA GGAGATGGCT GCCCTGGACG AGTCCGTGGT CCGCCTGACT 
AAGGAGAAGA AGGCGTTGCA GGAGGCTCAC CAGCAGGCTC TGGGTGACCT GCAGGCTGAA 
GAGGACCGTG TGAGTGCGCT GGCCAAGGCC AAGATCCGGT TGGAGCAGCA AGTAGAGGAC 
CTGGAGTGCT CCCTGGAGCA AGAGAAGAAG CTTCGCATGG ATACAGAACG TGCCAAGCGC 
AAGCTCGAAG GAGACCTGAA GCTGACGCAG GAGACAGTGA CAGACACCAC ACAAGACAAG 
CAGCAGCTGG AGGAGAAGTT GAAGAAGAAG GATTCAGAGC TGAGTCAGCT GAGCCTGCGG 
GTGGAGGATG AGCAGCTCCT GGGGGCGCAG TTGCAGAAGA AGATCAAGGA GCTACAGGCG 
AGGGCTGAGG AGCTGGAGGA GGAGCTGGAG GCGGAGCGAG CAGCCCGTGC CCGTGTGGAA 
AAGCAGCGAG CTGAGGCAGC CCGAGAGCTG GAGGAGCTGA GTGAGAGGCT AGAGGAGGCT 
GGTGGAGCCT CTGCCGGGCA GCGTGAGGGT TGCCGCAAGC GTGAGGCTGA GTTGGGCCGA 
CTGCGGCGGG AGCTGGAGGA GACAGTTCTG AGACACGAGG CCACTGTGGC TGCCCTGCGC 
CGCAAGCAGG CTGATAGCGC CGCAGAGTTG AGCGAGCAGG TAGACAGCCT CCAGCGGATC 
CGGCAGAAAC TGGAGAAGGA AAAGAGTGAG CTGCGCATGG AGGTGGATGA TCTGGGGGCC 
AGCGTGGAAA CTCTGGCCCG TGGCAAGGCC AGTGCAGAGA AGCTGTGCCG GACCTATGAG 
GACCAGCTCA GCGAGGCCAA GATCAAAGTG GAGGAGCTGC AGCGGCAGCT GACTGACGCC 
AGCACCCAGC GGGGGCGGTT ACAAACGGAG AACGGGGAGC TGGGCCGCCT GCTTGAGGAG 
AAGGAGTCTA TGATCAGCCA GCTGAGCCGC GGGAAGACCT CAGCCGCTCA GAGTCTGGAG 
GAACTTCGGC GGCAGTTGGA GGAAGAAAGC AAGGCCAAGG GTGCGCTGGC TCACGCAGTG 
CAGGCTCTGC GGCACGACTG CGACCTCCTG CGAGAGCAGC ATGAGGAAGA GTCCGAGGCG 
CAGGCTGAGC TGCAGCGGCT GCTGTCCAAA GCCAACGCTG AGGTGGCACA GTGGAGGAGC 
AAGTATGAGG CGGACGCCAT CCAAAGGACG GAGGAGCTGG AGGAGGCCAA GAAGAAGCTG 
GCCCTGAGGC TGCAGGAGGC AGAAGAAGGC GTAGAGGCTG CGAATGCCAA GTGCTCCTCA 
CTGGAGAAGG CCAAGCTGCG GCTGCAGACA GAGTCCGAGG ACGTGACCTT GGAGCTGGAG 
CGGGCCACCT CAGCCGCTGC TGCACTGGAT AAGAAGCAGC GGCACTTGGA GCGGGCCCTG 
GAGGAACGGA GGCGGCAGGA GGAGGAGATG CAGCGGGAAC TGGAGGCGGC GCAGAGGGAG 
GCTCGAGGTC TGGGCACCGA GCTCTTTCGA TTGCGGCACA GCCATGAGGA GGCGCTTGAG 
GCTCTGGAGA CACTCAAGCG GGAGAACAAG AATCTGCAGG AGGAGATCAG CGACCTTACA 
GACCAGGTCA GCCTCAGCGG GAAGAGCATC CAGGAACTGG AGAAGGCCAA GAAGGCGCTG 
GAAGGGGAGA AGAGTGAGCT GCAGGCTGCG CTGGAGGAGG CTGAGGGGGC GCTGGAGCTG 
GAAGAGACAA AGACCCTGCG GATCCAGTTG GAGTTGTCCC AGGTCAAGGC GGAAGTGGAC 
CGAAAGCTGG CGGAGAAGGA TGAGGAGTGT ACTAACCTGA GGCGCAACCA CCAGCGGGCT 
GTGGAGTCCT TGCAGGCCTC CCTGGATGCC GAGACTAGGG CCCGCAATGA AGCACTGCGT 
CTCAAGAAGA AGATGGAGGG GGACCTCAAT GACCTGGAGT TACAGCTGGG CCACGCAACC 
CGCCAGGCCA TGGAGGCACA GGCAGCCACG AGGCTGCTTC AGGCCCAACT CAAGGAGGAG 
CAAGCAGGGC GTGATGAGGA GCAGAGGCTG GCTGCCGAGC TCCGCGAGCA GGGCCAGGCC 
CTCGAGCGCA GGGCTGCACT GCTGGCATCT GAGCTGGAAG AGCTTCGGGC CGCCCTGGAA 
CAGGGCGAGC GCAGCCGAAG GCTGGCTGAA CAGGAGCTGT TGGAGGCCAC AGAGCGCCTC 
AACCTACTGC ATTCACAGAA CACAGGCCTC CTGAACCAGA AAAAGAAGCT GGAGGTGGAC 
TTGGCCCAGC TGAGTGGGGA GGTGGAGGAG GCTGCGCAGG AGAGGCGGGA AGCTGAGGAG 
AAGGCCAAAA AGGCCATCAC GGATGCAGCC ATGATGGCTG AGGAGCTGAA AAAGGAGCAG 
GACACAAGTG CACACCTCGA ACGGATGAAG AAGACCCTGG AACAGACAGT GCGGGAGCTG 
CAGGCCCGCC TTGAGGAAGC AGAGCAGGCC GCACTCCGGG GTGGGAAGAA GCAAGTGCAG 
AAGCTGGAAG CCAAGGTGCG GGAACTGGAG GCAGAGCTTG ACGCAGAGCA GAAGAAGCAC 
GCTGAGGCCC TCAAGGGTGT GCGCAAACAT GAGCGCAGGG TCAAGGAGCT TGCGTACCAG 
ACTGAGGAAG ACAGGAAGAA CCTGGCTCGC ATGCAGGACC TAGTGGACAA GCTGCAGAGC 
AAGGTCAAGA GCTACAAGCG TCAGTTTGAG GAGGCGGAGC AGCAGGCCAG TACCAACCTG 
GCTAAGTACC GCAAGGCCCA GCACGAGTTA GACGACGCTG AGGAGCGGGC AGACATGGCA 
GAGACCCAGG CCAACAAGCT GCGGGCAAGG TCCAGGGATG CCCTGGGTCC CAAGCACAAG 
GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017447272.1
CDS	153..5978
Translation	MMDMNELGESACYLRQGYQEMMKVHTVPWDGKKRVWVPDEQDAYVEAEVKTEATGGRVTVETKDQKVLTVRETEMQPMNPPRFDLLEDMAMMTHLNEAAVLHNLRQRYARWMIYTYSGLFCVTINPYKWLPVYTATVVAAYKGKRRSEAPPHIYAVADNAYNDMLRNRENQSMLITGESGAGKTVNTKRVIQYFAIVAALGDGPGKKAQFLATKTGGTLEDQIIEANPAMEAFGNAKTLRNDNSSRFGKFIRIHFGPTGKLASADIDSYLLEKSRVIFQLPGERGYHVYYQILSGKKPELQDMLLLSMNPYDYHFCSQGVTTVDNMNDGEELIATDHAMDILGFSVDEKCACYKIVGALLHFGNMKFKQKQREEQAEADGTESADKAAYLMGVSSGDLLKGLLHPRVRVGNEYVTKGQSVEQVVFAVGALAKATYDRLFRWLVSRINQTLDTKLPRQFFIGVLDIAGFEIFEFNSFEQLCINFTNEKLQQFFNQHMFVLEQEEYKREGIDWVFIDFGLDLQPCIDLIEKPLGILSILEEECMFPKASDASFRAKLYDNHSGKSPNFQQPRPDKKRKYQAHFEVVHYAGVVPYSIVGWLEKNKDPLNETVVPIFQKSQNRLLATLYENYAGSCSTEPPKSGVKEKRKKAASFQTVSQLHKENLNKLMTNLRATQPHFVRCIVPNENKTPGVMDSFLVLHQLRCNGVLEGIRICRQGFPNRLLYADFRQRYRILNPSAIPDDTFVDSRKATEKLLGSLDIDHTQYQFGHTKVFFKAGLLGILEELRDQRLAKVLTLLQARSRGRLMRLEYQRMLGGRDALFTIQWNIRAFNAVKNWSWMKLFFKMKPLLRSAQAEEELAALRAELRGLRGALATAEAKRQELEETQVSVTQEKNDLALQLQAEQDNLADAEERCHLLIKSKVQLEAKVKELNERLEDEEEVNADLAARRRKLEDECTELKKDIDDLELTLAKAEKEKQATENKVKNLTEEMAALDESVVRLTKEKKALQEAHQQALGDLQAEEDRVSALAKAKIRLEQQVEDLECSLEQEKKLRMDTERAKRKLEGDLKLTQETVTDTTQDKQQLEEKLKKKDSELSQLSLRVEDEQLLGAQLQKKIKELQARAEELEEELEAERAARARVEKQRAEAARELEELSERLEEAGGASAGQREGCRKREAELGRLRRELEETVLRHEATVAALRRKQADSAAELSEQVDSLQRIRQKLEKEKSELRMEVDDLGASVETLARGKASAEKLCRTYEDQLSEAKIKVEELQRQLTDASTQRGRLQTENGELGRLLEEKESMISQLSRGKTSAAQSLEELRRQLEEESKAKGALAHAVQALRHDCDLLREQHEEESEAQAELQRLLSKANAEVAQWRSKYEADAIQRTEELEEAKKKLALRLQEAEEGVEAANAKCSSLEKAKLRLQTESEDVTLELERATSAAAALDKKQRHLERALEERRRQEEEMQRELEAAQREARGLGTELFRLRHSHEEALEALETLKRENKNLQEEISDLTDQVSLSGKSIQELEKAKKALEGEKSELQAALEEAEGALELEETKTLRIQLELSQVKAEVDRKLAEKDEECTNLRRNHQRAVESLQASLDAETRARNEALRLKKKMEGDLNDLELQLGHATRQAMEAQAATRLLQAQLKEEQAGRDEEQRLAAELREQGQALERRAALLASELEELRAALEQGERSRRLAEQELLEATERLNLLHSQNTGLLNQKKKLEVDLAQLSGEVEEAAQERREAEEKAKKAITDAAMMAEELKKEQDTSAHLERMKKTLEQTVRELQARLEEAEQAALRGGKKQVQKLEAKVRELEAELDAEQKKHAEALKGVRKHERRVKELAYQTEEDRKNLARMQDLVDKLQSKVKSYKRQFEEAEQQASTNLAKYRKAQHELDDAEERADMAETQANKLRARSRDALGPKHKE

Target ORF information:

RefSeq Version	XM_017591783.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus myosin heavy chain 7B (Myh7b), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017591783.1

ORF Insert Sequence:

ATGATGGATA TGAATGAACT TGGAGAATCA GCCTGCTACC TCCGCCAGGG ATACCAGGAA 
ATGATGAAGG TGCACACTGT CCCATGGGAT GGCAAGAAAC GGGTCTGGGT GCCTGATGAA 
CAGGATGCCT ATGTCGAAGC TGAGGTCAAG ACTGAAGCTA CTGGAGGCCG AGTCACTGTG 
GAGACCAAAG ACCAGAAGGT GCTGACAGTG CGTGAAACGG AAATGCAGCC CATGAACCCA 
CCCCGCTTCG ACTTACTGGA GGACATGGCC ATGATGACGC ACCTCAACGA GGCTGCAGTG 
CTGCACAACC TGCGCCAGCG TTATGCTCGC TGGATGATCT ACACATACTC GGGCCTCTTC 
TGTGTCACCA TTAACCCATA CAAATGGCTC CCGGTCTACA CAGCTACTGT GGTGGCCGCC 
TACAAGGGCA AGCGCCGCTC GGAGGCCCCG CCTCATATAT ATGCAGTGGC AGATAATGCC 
TACAATGACA TGCTCCGCAA CAGAGAGAAC CAGTCCATGC TGATCACCGG AGAGTCGGGG 
GCCGGTAAGA CGGTTAACAC CAAGCGGGTC ATTCAGTATT TTGCCATCGT CGCTGCCCTG 
GGAGACGGGC CGGGCAAGAA GGCACAATTT CTGGCAACAA AAACCGGGGG CACCCTTGAG 
GATCAAATCA TCGAGGCTAA CCCTGCCATG GAGGCTTTTG GTAACGCCAA GACTCTGAGG 
AACGACAACT CCTCCCGTTT TGGCAAGTTC ATCCGCATCC ACTTTGGTCC CACTGGGAAG 
CTGGCCTCTG CGGACATTGA CAGCTATCTC CTGGAAAAGT CTCGAGTGAT CTTCCAGCTG 
CCCGGTGAGC GTGGTTACCA TGTCTACTAC CAAATCCTCT CCGGGAAGAA GCCAGAGCTG 
CAGGACATGC TGCTCCTCTC CATGAACCCC TACGACTACC ACTTCTGCAG TCAGGGGGTC 
ACCACCGTGG ATAACATGAA CGACGGGGAG GAGCTTATCG CCACTGATCA TGCAATGGAC 
ATCCTGGGCT TCAGCGTGGA TGAGAAGTGC GCATGCTATA AGATCGTGGG CGCCCTCCTG 
CACTTTGGCA ACATGAAGTT CAAACAGAAG CAGAGAGAGG AACAGGCTGA GGCAGACGGC 
ACTGAGAGTG CCGACAAGGC TGCCTACCTG ATGGGAGTCA GCAGTGGAGA CCTCCTCAAA 
GGTCTTCTGC ACCCCCGGGT TCGTGTGGGA AACGAGTATG TGACCAAGGG CCAGAGTGTG 
GAGCAGGTGG TATTTGCTGT GGGGGCCCTG GCCAAGGCTA CCTATGACCG GTTGTTCCGA 
TGGCTGGTAT CTCGAATCAA TCAGACCCTG GACACAAAGC TCCCCCGTCA GTTCTTCATT 
GGGGTCCTGG ATATCGCTGG TTTTGAGATC TTTGAGTTTA ACAGCTTCGA GCAACTGTGT 
ATCAACTTCA CCAACGAGAA ACTGCAGCAG TTCTTCAACC AGCACATGTT CGTGCTGGAG 
CAGGAGGAGT ACAAACGGGA GGGCATTGAC TGGGTCTTCA TCGACTTCGG CCTTGACCTG 
CAGCCCTGCA TCGACCTCAT CGAGAAGCCT CTGGGCATTC TGTCCATCCT GGAGGAGGAG 
TGCATGTTCC CCAAGGCTTC AGACGCAAGC TTCAGGGCCA AGCTCTATGA CAATCACTCT 
GGGAAGTCAC CTAATTTCCA GCAGCCTCGC CCAGACAAGA AACGCAAGTA CCAGGCTCAC 
TTCGAGGTGG TCCACTACGC AGGCGTGGTA CCTTATAGCA TTGTGGGCTG GCTGGAGAAA 
AACAAGGACC CCCTCAATGA GACCGTGGTC CCCATCTTCC AAAAGTCACA GAATAGGCTC 
TTGGCCACCC TCTATGAGAA CTATGCAGGT TCTTGCTCCA CCGAGCCCCC CAAGTCTGGG 
GTGAAAGAGA AACGAAAGAA AGCAGCATCA TTTCAGACAG TGTCTCAGCT TCACAAGGAG 
AACCTCAACA AGCTTATGAC CAACCTGCGG GCCACACAGC CCCACTTCGT CCGTTGTATC 
GTCCCCAACG AGAACAAGAC TCCAGGGGTC ATGGATTCCT TCTTGGTGCT ACACCAGCTA 
CGCTGTAACG GGGTCCTAGA GGGGATCCGG ATCTGCCGCC AAGGGTTCCC CAACAGACTG 
CTGTATGCTG ACTTCAGGCA GCGGTACCGA ATCCTGAACC CCAGTGCCAT CCCAGATGAC 
ACCTTTGTGG ACAGCAGGAA GGCCACAGAG AAGCTGCTGG GCTCTCTGGA CATTGACCAC 
ACCCAGTATC AGTTTGGCCA CACCAAGGTG TTCTTCAAGG CTGGGCTTCT AGGCATCCTG 
GAGGAGCTCC GGGACCAGCG TCTGGCTAAG GTGCTGACAC TGCTGCAGGC TCGGAGCCGG 
GGCCGCCTCA TGCGTCTGGA GTACCAGCGC ATGCTAGGAG GAAGGGATGC CCTGTTCACC 
ATCCAGTGGA ACATCCGGGC CTTCAATGCC GTCAAGAACT GGTCCTGGAT GAAGCTCTTC 
TTCAAGATGA AGCCACTGCT GCGCTCAGCA CAGGCAGAGG AAGAGCTGGC AGCACTTCGG 
GCAGAACTGC GGGGGCTGCG GGGGGCCCTG GCTACGGCTG AGGCCAAGCG TCAGGAGCTG 
GAGGAGACTC AAGTCAGTGT CACTCAGGAG AAGAATGACC TGGCCCTGCA GCTGCAGGCA 
GAGCAGGACA ACCTAGCGGA TGCGGAGGAG CGCTGCCACT TGCTCATCAA GTCCAAGGTG 
CAGCTCGAGG CGAAGGTGAA GGAGCTGAAC GAGCGGCTGG AGGATGAGGA GGAAGTGAAC 
GCTGACCTGG CCGCACGCAG GCGCAAGCTG GAGGATGAGT GCACGGAGCT CAAGAAGGAC 
ATTGATGACC TGGAGCTGAC CCTGGCCAAG GCTGAGAAGG AGAAACAAGC CACCGAGAAT 
AAGGTGAAGA ACCTGACAGA GGAGATGGCT GCCCTGGACG AGTCCGTGGT CCGCCTGACT 
AAGGAGAAGA AGGCGTTGCA GGAGGCTCAC CAGCAGGCTC TGGGTGACCT GCAGGCTGAA 
GAGGACCGTG TGAGTGCGCT GGCCAAGGCC AAGATCCGGT TGGAGCAGCA AGTAGAGGAC 
CTGGAGTGCT CCCTGGAGCA AGAGAAGAAG CTTCGCATGG ATACAGAACG TGCCAAGCGC 
AAGCTCGAAG GAGACCTGAA GCTGACGCAG GAGACAGTGA CAGACACCAC ACAAGACAAG 
CAGCAGCTGG AGGAGAAGTT GAAGAAGAAG GATTCAGAGC TGAGTCAGCT GAGCCTGCGG 
GTGGAGGATG AGCAGCTCCT GGGGGCGCAG TTGCAGAAGA AGATCAAGGA GCTACAGGCG 
AGGGCTGAGG AGCTGGAGGA GGAGCTGGAG GCGGAGCGAG CAGCCCGTGC CCGTGTGGAA 
AAGCAGCGAG CTGAGGCAGC CCGAGAGCTG GAGGAGCTGA GTGAGAGGCT AGAGGAGGCT 
GGTGGAGCCT CTGCCGGGCA GCGTGAGGGT TGCCGCAAGC GTGAGGCTGA GTTGGGCCGA 
CTGCGGCGGG AGCTGGAGGA GACAGTTCTG AGACACGAGG CCACTGTGGC TGCCCTGCGC 
CGCAAGCAGG CTGATAGCGC CGCAGAGTTG AGCGAGCAGG TAGACAGCCT CCAGCGGATC 
CGGCAGAAAC TGGAGAAGGA AAAGAGTGAG CTGCGCATGG AGGTGGATGA TCTGGGGGCC 
AGCGTGGAAA CTCTGGCCCG TGGCAAGGCC AGTGCAGAGA AGCTGTGCCG GACCTATGAG 
GACCAGCTCA GCGAGGCCAA GATCAAAGTG GAGGAGCTGC AGCGGCAGCT GACTGACGCC 
AGCACCCAGC GGGGGCGGTT ACAAACGGAG AACGGGGAGC TGGGCCGCCT GCTTGAGGAG 
AAGGAGTCTA TGATCAGCCA GCTGAGCCGC GGGAAGACCT CAGCCGCTCA GAGTCTGGAG 
GAACTTCGGC GGCAGTTGGA GGAAGAAAGC AAGGCCAAGG GTGCGCTGGC TCACGCAGTG 
CAGGCTCTGC GGCACGACTG CGACCTCCTG CGAGAGCAGC ATGAGGAAGA GTCCGAGGCG 
CAGGCTGAGC TGCAGCGGCT GCTGTCCAAA GCCAACGCTG AGGTGGCACA GTGGAGGAGC 
AAGTATGAGG CGGACGCCAT CCAAAGGACG GAGGAGCTGG AGGAGGCCAA GAAGAAGCTG 
GCCCTGAGGC TGCAGGAGGC AGAAGAAGGC GTAGAGGCTG CGAATGCCAA GTGCTCCTCA 
CTGGAGAAGG CCAAGCTGCG GCTGCAGACA GAGTCCGAGG ACGTGACCTT GGAGCTGGAG 
CGGGCCACCT CAGCCGCTGC TGCACTGGAT AAGAAGCAGC GGCACTTGGA GCGGGCCCTG 
GAGGAACGGA GGCGGCAGGA GGAGGAGATG CAGCGGGAAC TGGAGGCGGC GCAGAGGGAG 
GCTCGAGGTC TGGGCACCGA GCTCTTTCGA TTGCGGCACA GCCATGAGGA GGCGCTTGAG 
GCTCTGGAGA CACTCAAGCG GGAGAACAAG AATCTGCAGG AGGAGATCAG CGACCTTACA 
GACCAGGTCA GCCTCAGCGG GAAGAGCATC CAGGAACTGG AGAAGGCCAA GAAGGCGCTG 
GAAGGGGAGA AGAGTGAGCT GCAGGCTGCG CTGGAGGAGG CTGAGGGGGC GCTGGAGCTG 
GAAGAGACAA AGACCCTGCG GATCCAGTTG GAGTTGTCCC AGGTCAAGGC GGAAGTGGAC 
CGAAAGCTGG CGGAGAAGGA TGAGGAGTGT ACTAACCTGA GGCGCAACCA CCAGCGGGCT 
GTGGAGTCCT TGCAGGCCTC CCTGGATGCC GAGACTAGGG CCCGCAATGA AGCACTGCGT 
CTCAAGAAGA AGATGGAGGG GGACCTCAAT GACCTGGAGT TACAGCTGGG CCACGCAACC 
CGCCAGGCCA TGGAGGCACA GGCAGCCACG AGGCTGCTTC AGGCCCAACT CAAGGAGGAG 
CAAGCAGGGC GTGATGAGGA GCAGAGGCTG GCTGCCGAGC TCCGCGAGCA GGGCCAGGCC 
CTCGAGCGCA GGGCTGCACT GCTGGCATCT GAGCTGGAAG AGCTTCGGGC CGCCCTGGAA 
CAGGGCGAGC GCAGCCGAAG GCTGGCTGAA CAGGAGCTGT TGGAGGCCAC AGAGCGCCTC 
AACCTACTGC ATTCACAGAA CACAGGCCTC CTGAACCAGA AAAAGAAGCT GGAGGTGGAC 
TTGGCCCAGC TGAGTGGGGA GGTGGAGGAG GCTGCGCAGG AGAGGCGGGA AGCTGAGGAG 
AAGGCCAAAA AGGCCATCAC GGATGCAGCC ATGATGGCTG AGGAGCTGAA AAAGGAGCAG 
GACACAAGTG CACACCTCGA ACGGATGAAG AAGACCCTGG AACAGACAGT GCGGGAGCTG 
CAGGCCCGCC TTGAGGAAGC AGAGCAGGCC GCACTCCGGG GTGGGAAGAA GCAAGTGCAG 
AAGCTGGAAG CCAAGGTGCG GGAACTGGAG GCAGAGCTTG ACGCAGAGCA GAAGAAGCAC 
GCTGAGGCCC TCAAGGGTGT GCGCAAACAT GAGCGCAGGG TCAAGGAGCT TGCGTACCAG 
ACTGAGGAAG ACAGGAAGAA CCTGGCTCGC ATGCAGGACC TAGTGGACAA GCTGCAGAGC 
AAGGTCAAGA GCTACAAGCG TCAGTTTGAG GAGGCGGAGC AGCAGGCCAG TACCAACCTG 
GCTAAGTACC GCAAGGCCCA GCACGAGTTA GACGACGCTG AGGAGCGGGC AGACATGGCA 
GAGACCCAGG CCAACAAGCT GCGGGCAAGG TCCAGGGATG CCCTGGGTCC CAAGCACAAG 
GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Myh7b ( XM_008762340.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008760562.1 Rattus norvegicus myosin heavy chain 7B (Myh7b), transcript variant X1, mRNA.

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CloneID	ORa47041
Clone ID Related Accession (Same CDS sequence)	XM_008762340.2 , XM_008762339.2 , XM_017591782.1
Accession Version	XM_008762340.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6099bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	myosin-7B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005102.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008762340.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGATA TGAATGAACT TGGAGAATCA GCCTGCTACC TCCGCCAGGG ATACCAGGAA 
ATGATGAAGG TGCACACTGT CCCATGGGAT GGCAAGAAAC GGGTCTGGGT GCCTGATGAA 
CAGGATGCCT ATGTCGAAGC TGAGGTCAAG ACTGAAGCTA CTGGAGGCCG AGTCACTGTG 
GAGACCAAAG ACCAGAAGGT GCTGACAGTG CGTGAAACGG AAATGCAGCC CATGAACCCA 
CCCCGCTTCG ACTTACTGGA GGACATGGCC ATGATGACGC ACCTCAACGA GGCTGCAGTG 
CTGCACAACC TGCGCCAGCG TTATGCTCGC TGGATGATCT ACACATACTC GGGCCTCTTC 
TGTGTCACCA TTAACCCATA CAAATGGCTC CCGGTCTACA CAGCTACTGT GGTGGCCGCC 
TACAAGGGCA AGCGCCGCTC GGAGGCCCCG CCTCATATAT ATGCAGTGGC AGATAATGCC 
TACAATGACA TGCTCCGCAA CAGAGAGAAC CAGTCCATGC TGATCACCGG AGAGTCGGGG 
GCCGGTAAGA CGGTTAACAC CAAGCGGGTC ATTCAGTATT TTGCCATCGT CGCTGCCCTG 
GGAGACGGGC CGGGCAAGAA GGCACAATTT CTGGCAACAA AAACCGGGGG CACCCTTGAG 
GATCAAATCA TCGAGGCTAA CCCTGCCATG GAGGCTTTTG GTAACGCCAA GACTCTGAGG 
AACGACAACT CCTCCCGTTT TGGCAAGTTC ATCCGCATCC ACTTTGGTCC CACTGGGAAG 
CTGGCCTCTG CGGACATTGA CAGCTATCTC CTGGAAAAGT CTCGAGTGAT CTTCCAGCTG 
CCCGGTGAGC GTGGTTACCA TGTCTACTAC CAAATCCTCT CCGGGAAGAA GCCAGAGCTG 
CAGGACATGC TGCTCCTCTC CATGAACCCC TACGACTACC ACTTCTGCAG TCAGGGGGTC 
ACCACCGTGG ATAACATGAA CGACGGGGAG GAGCTTATCG CCACTGATCA TGCAATGGAC 
ATCCTGGGCT TCAGCGTGGA TGAGAAGTGC GCATGCTATA AGATCGTGGG CGCCCTCCTG 
CACTTTGGCA ACATGAAGTT CAAACAGAAG CAGAGAGAGG AACAGGCTGA GGCAGACGGC 
ACTGAGAGTG CCGACAAGGC TGCCTACCTG ATGGGAGTCA GCAGTGGAGA CCTCCTCAAA 
GGTCTTCTGC ACCCCCGGGT TCGTGTGGGA AACGAGTATG TGACCAAGGG CCAGAGTGTG 
GAGCAGGTGG TATTTGCTGT GGGGGCCCTG GCCAAGGCTA CCTATGACCG GTTGTTCCGA 
TGGCTGGTAT CTCGAATCAA TCAGACCCTG GACACAAAGC TCCCCCGTCA GTTCTTCATT 
GGGGTCCTGG ATATCGCTGG TTTTGAGATC TTTGAGTTTA ACAGCTTCGA GCAACTGTGT 
ATCAACTTCA CCAACGAGAA ACTGCAGCAG TTCTTCAACC AGCACATGTT CGTGCTGGAG 
CAGGAGGAGT ACAAACGGGA GGGCATTGAC TGGGTCTTCA TCGACTTCGG CCTTGACCTG 
CAGCCCTGCA TCGACCTCAT CGAGAAGCCT CTGGGCATTC TGTCCATCCT GGAGGAGGAG 
TGCATGTTCC CCAAGGCTTC AGACGCAAGC TTCAGGGCCA AGCTCTATGA CAATCACTCT 
GGGAAGTCAC CTAATTTCCA GCAGCCTCGC CCAGACAAGA AACGCAAGTA CCAGGCTCAC 
TTCGAGGTGG TCCACTACGC AGGCGTGGTA CCTTATAGCA TTGTGGGCTG GCTGGAGAAA 
AACAAGGACC CCCTCAATGA GACCGTGGTC CCCATCTTCC AAAAGTCACA GAATAGGCTC 
TTGGCCACCC TCTATGAGAA CTATGCAGGT TCTTGCTCCA CCGAGCCCCC CAAGTCTGGG 
GTGAAAGAGA AACGAAAGAA AGCAGCATCA TTTCAGACAG TGTCTCAGCT TCACAAGGAG 
AACCTCAACA AGCTTATGAC CAACCTGCGG GCCACACAGC CCCACTTCGT CCGTTGTATC 
GTCCCCAACG AGAACAAGAC TCCAGGGGTC ATGGATTCCT TCTTGGTGCT ACACCAGCTA 
CGCTGTAACG GGGTCCTAGA GGGGATCCGG ATCTGCCGCC AAGGGTTCCC CAACAGACTG 
CTGTATGCTG ACTTCAGGCA GCGGTACCGA ATCCTGAACC CCAGTGCCAT CCCAGATGAC 
ACCTTTGTGG ACAGCAGGAA GGCCACAGAG AAGCTGCTGG GCTCTCTGGA CATTGACCAC 
ACCCAGTATC AGTTTGGCCA CACCAAGGTG TTCTTCAAGG CTGGGCTTCT AGGCATCCTG 
GAGGAGCTCC GGGACCAGCG TCTGGCTAAG GTGCTGACAC TGCTGCAGGC TCGGAGCCGG 
GGCCGCCTCA TGCGTCTGGA GTACCAGCGC ATGCTAGGAG GAAGGGATGC CCTGTTCACC 
ATCCAGTGGA ACATCCGGGC CTTCAATGCC GTCAAGAACT GGTCCTGGAT GAAGCTCTTC 
TTCAAGATGA AGCCACTGCT GCGCTCAGCA CAGGCAGAGG AAGAGCTGGC AGCACTTCGG 
GCAGAACTGC GGGGGCTGCG GGGGGCCCTG GCTACGGCTG AGGCCAAGCG TCAGGAGCTG 
GAGGAGACTC AAGTCAGTGT CACTCAGGAG AAGAATGACC TGGCCCTGCA GCTGCAGGCA 
GAGCAGGACA ACCTAGCGGA TGCGGAGGAG CGCTGCCACT TGCTCATCAA GTCCAAGGTG 
CAGCTCGAGG CGAAGGTGAA GGAGCTGAAC GAGCGGCTGG AGGATGAGGA GGAAGTGAAC 
GCTGACCTGG CCGCACGCAG GCGCAAGCTG GAGGATGAGT GCACGGAGCT CAAGAAGGAC 
ATTGATGACC TGGAGCTGAC CCTGGCCAAG GCTGAGAAGG AGAAACAAGC CACCGAGAAT 
AAGGTGAAGA ACCTGACAGA GGAGATGGCT GCCCTGGACG AGTCCGTGGT CCGCCTGACT 
AAGGAGAAGA AGGCGTTGCA GGAGGCTCAC CAGCAGGCTC TGGGTGACCT GCAGGCTGAA 
GAGGACCGTG TGAGTGCGCT GGCCAAGGCC AAGATCCGGT TGGAGCAGCA AGTAGAGGAC 
CTGGAGTGCT CCCTGGAGCA AGAGAAGAAG CTTCGCATGG ATACAGAACG TGCCAAGCGC 
AAGCTCGAAG GAGACCTGAA GCTGACGCAG GAGACAGTGA CAGACACCAC ACAAGACAAG 
CAGCAGCTGG AGGAGAAGTT GAAGAAGAAG GATTCAGAGC TGAGTCAGCT GAGCCTGCGG 
GTGGAGGATG AGCAGCTCCT GGGGGCGCAG TTGCAGAAGA AGATCAAGGA GCTACAGGCG 
AGGGCTGAGG AGCTGGAGGA GGAGCTGGAG GCGGAGCGAG CAGCCCGTGC CCGTGTGGAA 
AAGCAGCGAG CTGAGGCAGC CCGAGAGCTG GAGGAGCTGA GTGAGAGGCT AGAGGAGGCT 
GGTGGAGCCT CTGCCGGGCA GCGTGAGGGT TGCCGCAAGC GTGAGGCTGA GTTGGGCCGA 
CTGCGGCGGG AGCTGGAGGA GACAGTTCTG AGACACGAGG CCACTGTGGC TGCCCTGCGC 
CGCAAGCAGG CTGATAGCGC CGCAGAGTTG AGCGAGCAGG TAGACAGCCT CCAGCGGATC 
CGGCAGAAAC TGGAGAAGGA AAAGAGTGAG CTGCGCATGG AGGTGGATGA TCTGGGGGCC 
AGCGTGGAAA CTCTGGCCCG TGGCAAGGCC AGTGCAGAGA AGCTGTGCCG GACCTATGAG 
GACCAGCTCA GCGAGGCCAA GATCAAAGTG GAGGAGCTGC AGCGGCAGCT GACTGACGCC 
AGCACCCAGC GGGGGCGGTT ACAAACGGAG AACGGGGAGC TGGGCCGCCT GCTTGAGGAG 
AAGGAGTCTA TGATCAGCCA GCTGAGCCGC GGGAAGACCT CAGCCGCTCA GAGTCTGGAG 
GAACTTCGGC GGCAGTTGGA GGAAGAAAGC AAGGCCAAGG GTGCGCTGGC TCACGCAGTG 
CAGGCTCTGC GGCACGACTG CGACCTCCTG CGAGAGCAGC ATGAGGAAGA GTCCGAGGCG 
CAGGCTGAGC TGCAGCGGCT GCTGTCCAAA GCCAACGCTG AGGTGGCACA GTGGAGGAGC 
AAGTATGAGG CGGACGCCAT CCAAAGGACG GAGGAGCTGG AGGAGGCCAA GAAGAAGCTG 
GCCCTGAGGC TGCAGGAGGC AGAAGAAGGC GTAGAGGCTG CGAATGCCAA GTGCTCCTCA 
CTGGAGAAGG CCAAGCTGCG GCTGCAGACA GAGTCCGAGG ACGTGACCTT GGAGCTGGAG 
CGGGCCACCT CAGCCGCTGC TGCACTGGAT AAGAAGCAGC GGCACTTGGA GCGGGCCCTG 
GAGGAACGGA GGCGGCAGGA GGAGGAGATG CAGCGGGAAC TGGAGGCGGC GCAGAGGGAG 
GCTCGAGGTC TGGGCACCGA GCTCTTTCGA TTGCGGCACA GCCATGAGGA GGCGCTTGAG 
GCTCTGGAGA CACTCAAGCG GGAGAACAAG AATCTGCAGG AGGAGATCAG CGACCTTACA 
GACCAGGTCA GCCTCAGCGG GAAGAGCATC CAGGAACTGG AGAAGGCCAA GAAGGCGCTG 
GAAGGGGAGA AGAGTGAGCT GCAGGCTGCG CTGGAGGAGG CTGAGGGGGC GCTGGAGCTG 
GAAGAGACAA AGACCCTGCG GATCCAGTTG GAGTTGTCCC AGGTCAAGGC GGAAGTGGAC 
CGAAAGCTGG CGGAGAAGGA TGAGGAGTGT ACTAACCTGA GGCGCAACCA CCAGCGGGCT 
GTGGAGTCCT TGCAGGCCTC CCTGGATGCC GAGACTAGGG CCCGCAATGA AGCACTGCGT 
CTCAAGAAGA AGATGGAGGG GGACCTCAAT GACCTGGAGT TACAGCTGGG CCACGCAACC 
CGCCAGGCCA TGGAGGCACA GGCAGCCACG AGGCTGCTTC AGGCCCAACT CAAGGAGGAG 
CAAGCAGGGC GTGATGAGGA GCAGAGGCTG GCTGCCGAGC TCCGCGAGCA GGGCCAGGCC 
CTCGAGCGCA GGGCTGCACT GCTGGCATCT GAGCTGGAAG AGCTTCGGGC CGCCCTGGAA 
CAGGGCGAGC GCAGCCGAAG GCTGGCTGAA CAGGAGCTGT TGGAGGCCAC AGAGCGCCTC 
AACCTACTGC ATTCACAGAA CACAGGCCTC CTGAACCAGA AAAAGAAGCT GGAGGTGGAC 
TTGGCCCAGC TGAGTGGGGA GGTGGAGGAG GCTGCGCAGG AGAGGCGGGA AGCTGAGGAG 
AAGGCCAAAA AGGCCATCAC GGATGCAGCC ATGATGGCTG AGGAGCTGAA AAAGGAGCAG 
GACACAAGTG CACACCTCGA ACGGATGAAG AAGACCCTGG AACAGACAGT GCGGGAGCTG 
CAGGCCCGCC TTGAGGAAGC AGAGCAGGCC GCACTCCGGG GTGGGAAGAA GCAAGTGCAG 
AAGCTGGAAG CCAAGGTGCG GGAACTGGAG GCAGAGCTTG ACGCAGAGCA GAAGAAGCAC 
GCTGAGGCCC TCAAGGGTGT GCGCAAACAT GAGCGCAGGG TCAAGGAGCT TGCGTACCAG 
ACTGAGGAAG ACAGGAAGAA CCTGGCTCGC ATGCAGGACC TAGTGGACAA GCTGCAGAGC 
AAGGTCAAGA GCTACAAGCG TCAGTTTGAG GAGGCGGAGC AGCAGGCCAG TACCAACCTG 
GCTAAGTACC GCAAGGCCCA GCACGAGTTA GACGACGCTG AGGAGCGGGC AGACATGGCA 
GAGACCCAGG CCAACAAGCT GCGGGCAAGG TCCAGGGATG CCCTGGGTCC CAAGGTAAGG 
TGGCCACTGT GTTGGGTGGA CACTGGGGAG ACACGCACGG GTGACCTAGG TCAGCCTCTT 
TCTCATCTCT TAGCACAAGG AGTGAGAGAC TCCAGCTCTG ACGAGGGATA CCCCGTGCTC 
CTGTTGTCTT TCCACCTGCC ACTCCACAGA GCTCTGGAAC CCCAAATAAA CACCACATCT 
GCAGCAGCAG CCGCTGTCCT GTCCTTATTT CTTGCGGCTA GAGGAGCTGG GAACAGAACC 
TCATGGACAA AGTCAGGCCC ACGTCAATCT TTAAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008760562.1
CDS	154..6252
Translation	MMDMNELGESACYLRQGYQEMMKVHTVPWDGKKRVWVPDEQDAYVEAEVKTEATGGRVTVETKDQKVLTVRETEMQPMNPPRFDLLEDMAMMTHLNEAAVLHNLRQRYARWMIYTYSGLFCVTINPYKWLPVYTATVVAAYKGKRRSEAPPHIYAVADNAYNDMLRNRENQSMLITGESGAGKTVNTKRVIQYFAIVAALGDGPGKKAQFLATKTGGTLEDQIIEANPAMEAFGNAKTLRNDNSSRFGKFIRIHFGPTGKLASADIDSYLLEKSRVIFQLPGERGYHVYYQILSGKKPELQDMLLLSMNPYDYHFCSQGVTTVDNMNDGEELIATDHAMDILGFSVDEKCACYKIVGALLHFGNMKFKQKQREEQAEADGTESADKAAYLMGVSSGDLLKGLLHPRVRVGNEYVTKGQSVEQVVFAVGALAKATYDRLFRWLVSRINQTLDTKLPRQFFIGVLDIAGFEIFEFNSFEQLCINFTNEKLQQFFNQHMFVLEQEEYKREGIDWVFIDFGLDLQPCIDLIEKPLGILSILEEECMFPKASDASFRAKLYDNHSGKSPNFQQPRPDKKRKYQAHFEVVHYAGVVPYSIVGWLEKNKDPLNETVVPIFQKSQNRLLATLYENYAGSCSTEPPKSGVKEKRKKAASFQTVSQLHKENLNKLMTNLRATQPHFVRCIVPNENKTPGVMDSFLVLHQLRCNGVLEGIRICRQGFPNRLLYADFRQRYRILNPSAIPDDTFVDSRKATEKLLGSLDIDHTQYQFGHTKVFFKAGLLGILEELRDQRLAKVLTLLQARSRGRLMRLEYQRMLGGRDALFTIQWNIRAFNAVKNWSWMKLFFKMKPLLRSAQAEEELAALRAELRGLRGALATAEAKRQELEETQVSVTQEKNDLALQLQAEQDNLADAEERCHLLIKSKVQLEAKVKELNERLEDEEEVNADLAARRRKLEDECTELKKDIDDLELTLAKAEKEKQATENKVKNLTEEMAALDESVVRLTKEKKALQEAHQQALGDLQAEEDRVSALAKAKIRLEQQVEDLECSLEQEKKLRMDTERAKRKLEGDLKLTQETVTDTTQDKQQLEEKLKKKDSELSQLSLRVEDEQLLGAQLQKKIKELQARAEELEEELEAERAARARVEKQRAEAARELEELSERLEEAGGASAGQREGCRKREAELGRLRRELEETVLRHEATVAALRRKQADSAAELSEQVDSLQRIRQKLEKEKSELRMEVDDLGASVETLARGKASAEKLCRTYEDQLSEAKIKVEELQRQLTDASTQRGRLQTENGELGRLLEEKESMISQLSRGKTSAAQSLEELRRQLEEESKAKGALAHAVQALRHDCDLLREQHEEESEAQAELQRLLSKANAEVAQWRSKYEADAIQRTEELEEAKKKLALRLQEAEEGVEAANAKCSSLEKAKLRLQTESEDVTLELERATSAAAALDKKQRHLERALEERRRQEEEMQRELEAAQREARGLGTELFRLRHSHEEALEALETLKRENKNLQEEISDLTDQVSLSGKSIQELEKAKKALEGEKSELQAALEEAEGALELEETKTLRIQLELSQVKAEVDRKLAEKDEECTNLRRNHQRAVESLQASLDAETRARNEALRLKKKMEGDLNDLELQLGHATRQAMEAQAATRLLQAQLKEEQAGRDEEQRLAAELREQGQALERRAALLASELEELRAALEQGERSRRLAEQELLEATERLNLLHSQNTGLLNQKKKLEVDLAQLSGEVEEAAQERREAEEKAKKAITDAAMMAEELKKEQDTSAHLERMKKTLEQTVRELQARLEEAEQAALRGGKKQVQKLEAKVRELEAELDAEQKKHAEALKGVRKHERRVKELAYQTEEDRKNLARMQDLVDKLQSKVKSYKRQFEEAEQQASTNLAKYRKAQHELDDAEERADMAETQANKLRARSRDALGPKVRWPLCWVDTGETRTGDLGQPLSHLLAQGVRDSSSDEGYPVLLLSFHLPLHRALEPQINTTSAAAAAVLSLFLAARGAGNRTSWTKSGPRQSLK

Target ORF information:

RefSeq Version	XM_008762340.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus myosin heavy chain 7B (Myh7b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008762340.2

ORF Insert Sequence:

ATGATGGATA TGAATGAACT TGGAGAATCA GCCTGCTACC TCCGCCAGGG ATACCAGGAA 
ATGATGAAGG TGCACACTGT CCCATGGGAT GGCAAGAAAC GGGTCTGGGT GCCTGATGAA 
CAGGATGCCT ATGTCGAAGC TGAGGTCAAG ACTGAAGCTA CTGGAGGCCG AGTCACTGTG 
GAGACCAAAG ACCAGAAGGT GCTGACAGTG CGTGAAACGG AAATGCAGCC CATGAACCCA 
CCCCGCTTCG ACTTACTGGA GGACATGGCC ATGATGACGC ACCTCAACGA GGCTGCAGTG 
CTGCACAACC TGCGCCAGCG TTATGCTCGC TGGATGATCT ACACATACTC GGGCCTCTTC 
TGTGTCACCA TTAACCCATA CAAATGGCTC CCGGTCTACA CAGCTACTGT GGTGGCCGCC 
TACAAGGGCA AGCGCCGCTC GGAGGCCCCG CCTCATATAT ATGCAGTGGC AGATAATGCC 
TACAATGACA TGCTCCGCAA CAGAGAGAAC CAGTCCATGC TGATCACCGG AGAGTCGGGG 
GCCGGTAAGA CGGTTAACAC CAAGCGGGTC ATTCAGTATT TTGCCATCGT CGCTGCCCTG 
GGAGACGGGC CGGGCAAGAA GGCACAATTT CTGGCAACAA AAACCGGGGG CACCCTTGAG 
GATCAAATCA TCGAGGCTAA CCCTGCCATG GAGGCTTTTG GTAACGCCAA GACTCTGAGG 
AACGACAACT CCTCCCGTTT TGGCAAGTTC ATCCGCATCC ACTTTGGTCC CACTGGGAAG 
CTGGCCTCTG CGGACATTGA CAGCTATCTC CTGGAAAAGT CTCGAGTGAT CTTCCAGCTG 
CCCGGTGAGC GTGGTTACCA TGTCTACTAC CAAATCCTCT CCGGGAAGAA GCCAGAGCTG 
CAGGACATGC TGCTCCTCTC CATGAACCCC TACGACTACC ACTTCTGCAG TCAGGGGGTC 
ACCACCGTGG ATAACATGAA CGACGGGGAG GAGCTTATCG CCACTGATCA TGCAATGGAC 
ATCCTGGGCT TCAGCGTGGA TGAGAAGTGC GCATGCTATA AGATCGTGGG CGCCCTCCTG 
CACTTTGGCA ACATGAAGTT CAAACAGAAG CAGAGAGAGG AACAGGCTGA GGCAGACGGC 
ACTGAGAGTG CCGACAAGGC TGCCTACCTG ATGGGAGTCA GCAGTGGAGA CCTCCTCAAA 
GGTCTTCTGC ACCCCCGGGT TCGTGTGGGA AACGAGTATG TGACCAAGGG CCAGAGTGTG 
GAGCAGGTGG TATTTGCTGT GGGGGCCCTG GCCAAGGCTA CCTATGACCG GTTGTTCCGA 
TGGCTGGTAT CTCGAATCAA TCAGACCCTG GACACAAAGC TCCCCCGTCA GTTCTTCATT 
GGGGTCCTGG ATATCGCTGG TTTTGAGATC TTTGAGTTTA ACAGCTTCGA GCAACTGTGT 
ATCAACTTCA CCAACGAGAA ACTGCAGCAG TTCTTCAACC AGCACATGTT CGTGCTGGAG 
CAGGAGGAGT ACAAACGGGA GGGCATTGAC TGGGTCTTCA TCGACTTCGG CCTTGACCTG 
CAGCCCTGCA TCGACCTCAT CGAGAAGCCT CTGGGCATTC TGTCCATCCT GGAGGAGGAG 
TGCATGTTCC CCAAGGCTTC AGACGCAAGC TTCAGGGCCA AGCTCTATGA CAATCACTCT 
GGGAAGTCAC CTAATTTCCA GCAGCCTCGC CCAGACAAGA AACGCAAGTA CCAGGCTCAC 
TTCGAGGTGG TCCACTACGC AGGCGTGGTA CCTTATAGCA TTGTGGGCTG GCTGGAGAAA 
AACAAGGACC CCCTCAATGA GACCGTGGTC CCCATCTTCC AAAAGTCACA GAATAGGCTC 
TTGGCCACCC TCTATGAGAA CTATGCAGGT TCTTGCTCCA CCGAGCCCCC CAAGTCTGGG 
GTGAAAGAGA AACGAAAGAA AGCAGCATCA TTTCAGACAG TGTCTCAGCT TCACAAGGAG 
AACCTCAACA AGCTTATGAC CAACCTGCGG GCCACACAGC CCCACTTCGT CCGTTGTATC 
GTCCCCAACG AGAACAAGAC TCCAGGGGTC ATGGATTCCT TCTTGGTGCT ACACCAGCTA 
CGCTGTAACG GGGTCCTAGA GGGGATCCGG ATCTGCCGCC AAGGGTTCCC CAACAGACTG 
CTGTATGCTG ACTTCAGGCA GCGGTACCGA ATCCTGAACC CCAGTGCCAT CCCAGATGAC 
ACCTTTGTGG ACAGCAGGAA GGCCACAGAG AAGCTGCTGG GCTCTCTGGA CATTGACCAC 
ACCCAGTATC AGTTTGGCCA CACCAAGGTG TTCTTCAAGG CTGGGCTTCT AGGCATCCTG 
GAGGAGCTCC GGGACCAGCG TCTGGCTAAG GTGCTGACAC TGCTGCAGGC TCGGAGCCGG 
GGCCGCCTCA TGCGTCTGGA GTACCAGCGC ATGCTAGGAG GAAGGGATGC CCTGTTCACC 
ATCCAGTGGA ACATCCGGGC CTTCAATGCC GTCAAGAACT GGTCCTGGAT GAAGCTCTTC 
TTCAAGATGA AGCCACTGCT GCGCTCAGCA CAGGCAGAGG AAGAGCTGGC AGCACTTCGG 
GCAGAACTGC GGGGGCTGCG GGGGGCCCTG GCTACGGCTG AGGCCAAGCG TCAGGAGCTG 
GAGGAGACTC AAGTCAGTGT CACTCAGGAG AAGAATGACC TGGCCCTGCA GCTGCAGGCA 
GAGCAGGACA ACCTAGCGGA TGCGGAGGAG CGCTGCCACT TGCTCATCAA GTCCAAGGTG 
CAGCTCGAGG CGAAGGTGAA GGAGCTGAAC GAGCGGCTGG AGGATGAGGA GGAAGTGAAC 
GCTGACCTGG CCGCACGCAG GCGCAAGCTG GAGGATGAGT GCACGGAGCT CAAGAAGGAC 
ATTGATGACC TGGAGCTGAC CCTGGCCAAG GCTGAGAAGG AGAAACAAGC CACCGAGAAT 
AAGGTGAAGA ACCTGACAGA GGAGATGGCT GCCCTGGACG AGTCCGTGGT CCGCCTGACT 
AAGGAGAAGA AGGCGTTGCA GGAGGCTCAC CAGCAGGCTC TGGGTGACCT GCAGGCTGAA 
GAGGACCGTG TGAGTGCGCT GGCCAAGGCC AAGATCCGGT TGGAGCAGCA AGTAGAGGAC 
CTGGAGTGCT CCCTGGAGCA AGAGAAGAAG CTTCGCATGG ATACAGAACG TGCCAAGCGC 
AAGCTCGAAG GAGACCTGAA GCTGACGCAG GAGACAGTGA CAGACACCAC ACAAGACAAG 
CAGCAGCTGG AGGAGAAGTT GAAGAAGAAG GATTCAGAGC TGAGTCAGCT GAGCCTGCGG 
GTGGAGGATG AGCAGCTCCT GGGGGCGCAG TTGCAGAAGA AGATCAAGGA GCTACAGGCG 
AGGGCTGAGG AGCTGGAGGA GGAGCTGGAG GCGGAGCGAG CAGCCCGTGC CCGTGTGGAA 
AAGCAGCGAG CTGAGGCAGC CCGAGAGCTG GAGGAGCTGA GTGAGAGGCT AGAGGAGGCT 
GGTGGAGCCT CTGCCGGGCA GCGTGAGGGT TGCCGCAAGC GTGAGGCTGA GTTGGGCCGA 
CTGCGGCGGG AGCTGGAGGA GACAGTTCTG AGACACGAGG CCACTGTGGC TGCCCTGCGC 
CGCAAGCAGG CTGATAGCGC CGCAGAGTTG AGCGAGCAGG TAGACAGCCT CCAGCGGATC 
CGGCAGAAAC TGGAGAAGGA AAAGAGTGAG CTGCGCATGG AGGTGGATGA TCTGGGGGCC 
AGCGTGGAAA CTCTGGCCCG TGGCAAGGCC AGTGCAGAGA AGCTGTGCCG GACCTATGAG 
GACCAGCTCA GCGAGGCCAA GATCAAAGTG GAGGAGCTGC AGCGGCAGCT GACTGACGCC 
AGCACCCAGC GGGGGCGGTT ACAAACGGAG AACGGGGAGC TGGGCCGCCT GCTTGAGGAG 
AAGGAGTCTA TGATCAGCCA GCTGAGCCGC GGGAAGACCT CAGCCGCTCA GAGTCTGGAG 
GAACTTCGGC GGCAGTTGGA GGAAGAAAGC AAGGCCAAGG GTGCGCTGGC TCACGCAGTG 
CAGGCTCTGC GGCACGACTG CGACCTCCTG CGAGAGCAGC ATGAGGAAGA GTCCGAGGCG 
CAGGCTGAGC TGCAGCGGCT GCTGTCCAAA GCCAACGCTG AGGTGGCACA GTGGAGGAGC 
AAGTATGAGG CGGACGCCAT CCAAAGGACG GAGGAGCTGG AGGAGGCCAA GAAGAAGCTG 
GCCCTGAGGC TGCAGGAGGC AGAAGAAGGC GTAGAGGCTG CGAATGCCAA GTGCTCCTCA 
CTGGAGAAGG CCAAGCTGCG GCTGCAGACA GAGTCCGAGG ACGTGACCTT GGAGCTGGAG 
CGGGCCACCT CAGCCGCTGC TGCACTGGAT AAGAAGCAGC GGCACTTGGA GCGGGCCCTG 
GAGGAACGGA GGCGGCAGGA GGAGGAGATG CAGCGGGAAC TGGAGGCGGC GCAGAGGGAG 
GCTCGAGGTC TGGGCACCGA GCTCTTTCGA TTGCGGCACA GCCATGAGGA GGCGCTTGAG 
GCTCTGGAGA CACTCAAGCG GGAGAACAAG AATCTGCAGG AGGAGATCAG CGACCTTACA 
GACCAGGTCA GCCTCAGCGG GAAGAGCATC CAGGAACTGG AGAAGGCCAA GAAGGCGCTG 
GAAGGGGAGA AGAGTGAGCT GCAGGCTGCG CTGGAGGAGG CTGAGGGGGC GCTGGAGCTG 
GAAGAGACAA AGACCCTGCG GATCCAGTTG GAGTTGTCCC AGGTCAAGGC GGAAGTGGAC 
CGAAAGCTGG CGGAGAAGGA TGAGGAGTGT ACTAACCTGA GGCGCAACCA CCAGCGGGCT 
GTGGAGTCCT TGCAGGCCTC CCTGGATGCC GAGACTAGGG CCCGCAATGA AGCACTGCGT 
CTCAAGAAGA AGATGGAGGG GGACCTCAAT GACCTGGAGT TACAGCTGGG CCACGCAACC 
CGCCAGGCCA TGGAGGCACA GGCAGCCACG AGGCTGCTTC AGGCCCAACT CAAGGAGGAG 
CAAGCAGGGC GTGATGAGGA GCAGAGGCTG GCTGCCGAGC TCCGCGAGCA GGGCCAGGCC 
CTCGAGCGCA GGGCTGCACT GCTGGCATCT GAGCTGGAAG AGCTTCGGGC CGCCCTGGAA 
CAGGGCGAGC GCAGCCGAAG GCTGGCTGAA CAGGAGCTGT TGGAGGCCAC AGAGCGCCTC 
AACCTACTGC ATTCACAGAA CACAGGCCTC CTGAACCAGA AAAAGAAGCT GGAGGTGGAC 
TTGGCCCAGC TGAGTGGGGA GGTGGAGGAG GCTGCGCAGG AGAGGCGGGA AGCTGAGGAG 
AAGGCCAAAA AGGCCATCAC GGATGCAGCC ATGATGGCTG AGGAGCTGAA AAAGGAGCAG 
GACACAAGTG CACACCTCGA ACGGATGAAG AAGACCCTGG AACAGACAGT GCGGGAGCTG 
CAGGCCCGCC TTGAGGAAGC AGAGCAGGCC GCACTCCGGG GTGGGAAGAA GCAAGTGCAG 
AAGCTGGAAG CCAAGGTGCG GGAACTGGAG GCAGAGCTTG ACGCAGAGCA GAAGAAGCAC 
GCTGAGGCCC TCAAGGGTGT GCGCAAACAT GAGCGCAGGG TCAAGGAGCT TGCGTACCAG 
ACTGAGGAAG ACAGGAAGAA CCTGGCTCGC ATGCAGGACC TAGTGGACAA GCTGCAGAGC 
AAGGTCAAGA GCTACAAGCG TCAGTTTGAG GAGGCGGAGC AGCAGGCCAG TACCAACCTG 
GCTAAGTACC GCAAGGCCCA GCACGAGTTA GACGACGCTG AGGAGCGGGC AGACATGGCA 
GAGACCCAGG CCAACAAGCT GCGGGCAAGG TCCAGGGATG CCCTGGGTCC CAAGGTAAGG 
TGGCCACTGT GTTGGGTGGA CACTGGGGAG ACACGCACGG GTGACCTAGG TCAGCCTCTT 
TCTCATCTCT TAGCACAAGG AGTGAGAGAC TCCAGCTCTG ACGAGGGATA CCCCGTGCTC 
CTGTTGTCTT TCCACCTGCC ACTCCACAGA GCTCTGGAAC CCCAAATAAA CACCACATCT 
GCAGCAGCAG CCGCTGTCCT GTCCTTATTT CTTGCGGCTA GAGGAGCTGG GAACAGAACC 
TCATGGACAA AGTCAGGCCC ACGTCAATCT TTAAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Myh7b ( XM_008762339.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008760561.1 Rattus norvegicus myosin heavy chain 7B (Myh7b), transcript variant X2, mRNA.

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CloneID	ORa47041
Clone ID Related Accession (Same CDS sequence)	XM_008762340.2 , XM_008762339.2 , XM_017591782.1
Accession Version	XM_008762339.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6099bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	myosin-7B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005102.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008762339.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGATA TGAATGAACT TGGAGAATCA GCCTGCTACC TCCGCCAGGG ATACCAGGAA 
ATGATGAAGG TGCACACTGT CCCATGGGAT GGCAAGAAAC GGGTCTGGGT GCCTGATGAA 
CAGGATGCCT ATGTCGAAGC TGAGGTCAAG ACTGAAGCTA CTGGAGGCCG AGTCACTGTG 
GAGACCAAAG ACCAGAAGGT GCTGACAGTG CGTGAAACGG AAATGCAGCC CATGAACCCA 
CCCCGCTTCG ACTTACTGGA GGACATGGCC ATGATGACGC ACCTCAACGA GGCTGCAGTG 
CTGCACAACC TGCGCCAGCG TTATGCTCGC TGGATGATCT ACACATACTC GGGCCTCTTC 
TGTGTCACCA TTAACCCATA CAAATGGCTC CCGGTCTACA CAGCTACTGT GGTGGCCGCC 
TACAAGGGCA AGCGCCGCTC GGAGGCCCCG CCTCATATAT ATGCAGTGGC AGATAATGCC 
TACAATGACA TGCTCCGCAA CAGAGAGAAC CAGTCCATGC TGATCACCGG AGAGTCGGGG 
GCCGGTAAGA CGGTTAACAC CAAGCGGGTC ATTCAGTATT TTGCCATCGT CGCTGCCCTG 
GGAGACGGGC CGGGCAAGAA GGCACAATTT CTGGCAACAA AAACCGGGGG CACCCTTGAG 
GATCAAATCA TCGAGGCTAA CCCTGCCATG GAGGCTTTTG GTAACGCCAA GACTCTGAGG 
AACGACAACT CCTCCCGTTT TGGCAAGTTC ATCCGCATCC ACTTTGGTCC CACTGGGAAG 
CTGGCCTCTG CGGACATTGA CAGCTATCTC CTGGAAAAGT CTCGAGTGAT CTTCCAGCTG 
CCCGGTGAGC GTGGTTACCA TGTCTACTAC CAAATCCTCT CCGGGAAGAA GCCAGAGCTG 
CAGGACATGC TGCTCCTCTC CATGAACCCC TACGACTACC ACTTCTGCAG TCAGGGGGTC 
ACCACCGTGG ATAACATGAA CGACGGGGAG GAGCTTATCG CCACTGATCA TGCAATGGAC 
ATCCTGGGCT TCAGCGTGGA TGAGAAGTGC GCATGCTATA AGATCGTGGG CGCCCTCCTG 
CACTTTGGCA ACATGAAGTT CAAACAGAAG CAGAGAGAGG AACAGGCTGA GGCAGACGGC 
ACTGAGAGTG CCGACAAGGC TGCCTACCTG ATGGGAGTCA GCAGTGGAGA CCTCCTCAAA 
GGTCTTCTGC ACCCCCGGGT TCGTGTGGGA AACGAGTATG TGACCAAGGG CCAGAGTGTG 
GAGCAGGTGG TATTTGCTGT GGGGGCCCTG GCCAAGGCTA CCTATGACCG GTTGTTCCGA 
TGGCTGGTAT CTCGAATCAA TCAGACCCTG GACACAAAGC TCCCCCGTCA GTTCTTCATT 
GGGGTCCTGG ATATCGCTGG TTTTGAGATC TTTGAGTTTA ACAGCTTCGA GCAACTGTGT 
ATCAACTTCA CCAACGAGAA ACTGCAGCAG TTCTTCAACC AGCACATGTT CGTGCTGGAG 
CAGGAGGAGT ACAAACGGGA GGGCATTGAC TGGGTCTTCA TCGACTTCGG CCTTGACCTG 
CAGCCCTGCA TCGACCTCAT CGAGAAGCCT CTGGGCATTC TGTCCATCCT GGAGGAGGAG 
TGCATGTTCC CCAAGGCTTC AGACGCAAGC TTCAGGGCCA AGCTCTATGA CAATCACTCT 
GGGAAGTCAC CTAATTTCCA GCAGCCTCGC CCAGACAAGA AACGCAAGTA CCAGGCTCAC 
TTCGAGGTGG TCCACTACGC AGGCGTGGTA CCTTATAGCA TTGTGGGCTG GCTGGAGAAA 
AACAAGGACC CCCTCAATGA GACCGTGGTC CCCATCTTCC AAAAGTCACA GAATAGGCTC 
TTGGCCACCC TCTATGAGAA CTATGCAGGT TCTTGCTCCA CCGAGCCCCC CAAGTCTGGG 
GTGAAAGAGA AACGAAAGAA AGCAGCATCA TTTCAGACAG TGTCTCAGCT TCACAAGGAG 
AACCTCAACA AGCTTATGAC CAACCTGCGG GCCACACAGC CCCACTTCGT CCGTTGTATC 
GTCCCCAACG AGAACAAGAC TCCAGGGGTC ATGGATTCCT TCTTGGTGCT ACACCAGCTA 
CGCTGTAACG GGGTCCTAGA GGGGATCCGG ATCTGCCGCC AAGGGTTCCC CAACAGACTG 
CTGTATGCTG ACTTCAGGCA GCGGTACCGA ATCCTGAACC CCAGTGCCAT CCCAGATGAC 
ACCTTTGTGG ACAGCAGGAA GGCCACAGAG AAGCTGCTGG GCTCTCTGGA CATTGACCAC 
ACCCAGTATC AGTTTGGCCA CACCAAGGTG TTCTTCAAGG CTGGGCTTCT AGGCATCCTG 
GAGGAGCTCC GGGACCAGCG TCTGGCTAAG GTGCTGACAC TGCTGCAGGC TCGGAGCCGG 
GGCCGCCTCA TGCGTCTGGA GTACCAGCGC ATGCTAGGAG GAAGGGATGC CCTGTTCACC 
ATCCAGTGGA ACATCCGGGC CTTCAATGCC GTCAAGAACT GGTCCTGGAT GAAGCTCTTC 
TTCAAGATGA AGCCACTGCT GCGCTCAGCA CAGGCAGAGG AAGAGCTGGC AGCACTTCGG 
GCAGAACTGC GGGGGCTGCG GGGGGCCCTG GCTACGGCTG AGGCCAAGCG TCAGGAGCTG 
GAGGAGACTC AAGTCAGTGT CACTCAGGAG AAGAATGACC TGGCCCTGCA GCTGCAGGCA 
GAGCAGGACA ACCTAGCGGA TGCGGAGGAG CGCTGCCACT TGCTCATCAA GTCCAAGGTG 
CAGCTCGAGG CGAAGGTGAA GGAGCTGAAC GAGCGGCTGG AGGATGAGGA GGAAGTGAAC 
GCTGACCTGG CCGCACGCAG GCGCAAGCTG GAGGATGAGT GCACGGAGCT CAAGAAGGAC 
ATTGATGACC TGGAGCTGAC CCTGGCCAAG GCTGAGAAGG AGAAACAAGC CACCGAGAAT 
AAGGTGAAGA ACCTGACAGA GGAGATGGCT GCCCTGGACG AGTCCGTGGT CCGCCTGACT 
AAGGAGAAGA AGGCGTTGCA GGAGGCTCAC CAGCAGGCTC TGGGTGACCT GCAGGCTGAA 
GAGGACCGTG TGAGTGCGCT GGCCAAGGCC AAGATCCGGT TGGAGCAGCA AGTAGAGGAC 
CTGGAGTGCT CCCTGGAGCA AGAGAAGAAG CTTCGCATGG ATACAGAACG TGCCAAGCGC 
AAGCTCGAAG GAGACCTGAA GCTGACGCAG GAGACAGTGA CAGACACCAC ACAAGACAAG 
CAGCAGCTGG AGGAGAAGTT GAAGAAGAAG GATTCAGAGC TGAGTCAGCT GAGCCTGCGG 
GTGGAGGATG AGCAGCTCCT GGGGGCGCAG TTGCAGAAGA AGATCAAGGA GCTACAGGCG 
AGGGCTGAGG AGCTGGAGGA GGAGCTGGAG GCGGAGCGAG CAGCCCGTGC CCGTGTGGAA 
AAGCAGCGAG CTGAGGCAGC CCGAGAGCTG GAGGAGCTGA GTGAGAGGCT AGAGGAGGCT 
GGTGGAGCCT CTGCCGGGCA GCGTGAGGGT TGCCGCAAGC GTGAGGCTGA GTTGGGCCGA 
CTGCGGCGGG AGCTGGAGGA GACAGTTCTG AGACACGAGG CCACTGTGGC TGCCCTGCGC 
CGCAAGCAGG CTGATAGCGC CGCAGAGTTG AGCGAGCAGG TAGACAGCCT CCAGCGGATC 
CGGCAGAAAC TGGAGAAGGA AAAGAGTGAG CTGCGCATGG AGGTGGATGA TCTGGGGGCC 
AGCGTGGAAA CTCTGGCCCG TGGCAAGGCC AGTGCAGAGA AGCTGTGCCG GACCTATGAG 
GACCAGCTCA GCGAGGCCAA GATCAAAGTG GAGGAGCTGC AGCGGCAGCT GACTGACGCC 
AGCACCCAGC GGGGGCGGTT ACAAACGGAG AACGGGGAGC TGGGCCGCCT GCTTGAGGAG 
AAGGAGTCTA TGATCAGCCA GCTGAGCCGC GGGAAGACCT CAGCCGCTCA GAGTCTGGAG 
GAACTTCGGC GGCAGTTGGA GGAAGAAAGC AAGGCCAAGG GTGCGCTGGC TCACGCAGTG 
CAGGCTCTGC GGCACGACTG CGACCTCCTG CGAGAGCAGC ATGAGGAAGA GTCCGAGGCG 
CAGGCTGAGC TGCAGCGGCT GCTGTCCAAA GCCAACGCTG AGGTGGCACA GTGGAGGAGC 
AAGTATGAGG CGGACGCCAT CCAAAGGACG GAGGAGCTGG AGGAGGCCAA GAAGAAGCTG 
GCCCTGAGGC TGCAGGAGGC AGAAGAAGGC GTAGAGGCTG CGAATGCCAA GTGCTCCTCA 
CTGGAGAAGG CCAAGCTGCG GCTGCAGACA GAGTCCGAGG ACGTGACCTT GGAGCTGGAG 
CGGGCCACCT CAGCCGCTGC TGCACTGGAT AAGAAGCAGC GGCACTTGGA GCGGGCCCTG 
GAGGAACGGA GGCGGCAGGA GGAGGAGATG CAGCGGGAAC TGGAGGCGGC GCAGAGGGAG 
GCTCGAGGTC TGGGCACCGA GCTCTTTCGA TTGCGGCACA GCCATGAGGA GGCGCTTGAG 
GCTCTGGAGA CACTCAAGCG GGAGAACAAG AATCTGCAGG AGGAGATCAG CGACCTTACA 
GACCAGGTCA GCCTCAGCGG GAAGAGCATC CAGGAACTGG AGAAGGCCAA GAAGGCGCTG 
GAAGGGGAGA AGAGTGAGCT GCAGGCTGCG CTGGAGGAGG CTGAGGGGGC GCTGGAGCTG 
GAAGAGACAA AGACCCTGCG GATCCAGTTG GAGTTGTCCC AGGTCAAGGC GGAAGTGGAC 
CGAAAGCTGG CGGAGAAGGA TGAGGAGTGT ACTAACCTGA GGCGCAACCA CCAGCGGGCT 
GTGGAGTCCT TGCAGGCCTC CCTGGATGCC GAGACTAGGG CCCGCAATGA AGCACTGCGT 
CTCAAGAAGA AGATGGAGGG GGACCTCAAT GACCTGGAGT TACAGCTGGG CCACGCAACC 
CGCCAGGCCA TGGAGGCACA GGCAGCCACG AGGCTGCTTC AGGCCCAACT CAAGGAGGAG 
CAAGCAGGGC GTGATGAGGA GCAGAGGCTG GCTGCCGAGC TCCGCGAGCA GGGCCAGGCC 
CTCGAGCGCA GGGCTGCACT GCTGGCATCT GAGCTGGAAG AGCTTCGGGC CGCCCTGGAA 
CAGGGCGAGC GCAGCCGAAG GCTGGCTGAA CAGGAGCTGT TGGAGGCCAC AGAGCGCCTC 
AACCTACTGC ATTCACAGAA CACAGGCCTC CTGAACCAGA AAAAGAAGCT GGAGGTGGAC 
TTGGCCCAGC TGAGTGGGGA GGTGGAGGAG GCTGCGCAGG AGAGGCGGGA AGCTGAGGAG 
AAGGCCAAAA AGGCCATCAC GGATGCAGCC ATGATGGCTG AGGAGCTGAA AAAGGAGCAG 
GACACAAGTG CACACCTCGA ACGGATGAAG AAGACCCTGG AACAGACAGT GCGGGAGCTG 
CAGGCCCGCC TTGAGGAAGC AGAGCAGGCC GCACTCCGGG GTGGGAAGAA GCAAGTGCAG 
AAGCTGGAAG CCAAGGTGCG GGAACTGGAG GCAGAGCTTG ACGCAGAGCA GAAGAAGCAC 
GCTGAGGCCC TCAAGGGTGT GCGCAAACAT GAGCGCAGGG TCAAGGAGCT TGCGTACCAG 
ACTGAGGAAG ACAGGAAGAA CCTGGCTCGC ATGCAGGACC TAGTGGACAA GCTGCAGAGC 
AAGGTCAAGA GCTACAAGCG TCAGTTTGAG GAGGCGGAGC AGCAGGCCAG TACCAACCTG 
GCTAAGTACC GCAAGGCCCA GCACGAGTTA GACGACGCTG AGGAGCGGGC AGACATGGCA 
GAGACCCAGG CCAACAAGCT GCGGGCAAGG TCCAGGGATG CCCTGGGTCC CAAGGTAAGG 
TGGCCACTGT GTTGGGTGGA CACTGGGGAG ACACGCACGG GTGACCTAGG TCAGCCTCTT 
TCTCATCTCT TAGCACAAGG AGTGAGAGAC TCCAGCTCTG ACGAGGGATA CCCCGTGCTC 
CTGTTGTCTT TCCACCTGCC ACTCCACAGA GCTCTGGAAC CCCAAATAAA CACCACATCT 
GCAGCAGCAG CCGCTGTCCT GTCCTTATTT CTTGCGGCTA GAGGAGCTGG GAACAGAACC 
TCATGGACAA AGTCAGGCCC ACGTCAATCT TTAAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008760561.1
CDS	400..6498
Translation	MMDMNELGESACYLRQGYQEMMKVHTVPWDGKKRVWVPDEQDAYVEAEVKTEATGGRVTVETKDQKVLTVRETEMQPMNPPRFDLLEDMAMMTHLNEAAVLHNLRQRYARWMIYTYSGLFCVTINPYKWLPVYTATVVAAYKGKRRSEAPPHIYAVADNAYNDMLRNRENQSMLITGESGAGKTVNTKRVIQYFAIVAALGDGPGKKAQFLATKTGGTLEDQIIEANPAMEAFGNAKTLRNDNSSRFGKFIRIHFGPTGKLASADIDSYLLEKSRVIFQLPGERGYHVYYQILSGKKPELQDMLLLSMNPYDYHFCSQGVTTVDNMNDGEELIATDHAMDILGFSVDEKCACYKIVGALLHFGNMKFKQKQREEQAEADGTESADKAAYLMGVSSGDLLKGLLHPRVRVGNEYVTKGQSVEQVVFAVGALAKATYDRLFRWLVSRINQTLDTKLPRQFFIGVLDIAGFEIFEFNSFEQLCINFTNEKLQQFFNQHMFVLEQEEYKREGIDWVFIDFGLDLQPCIDLIEKPLGILSILEEECMFPKASDASFRAKLYDNHSGKSPNFQQPRPDKKRKYQAHFEVVHYAGVVPYSIVGWLEKNKDPLNETVVPIFQKSQNRLLATLYENYAGSCSTEPPKSGVKEKRKKAASFQTVSQLHKENLNKLMTNLRATQPHFVRCIVPNENKTPGVMDSFLVLHQLRCNGVLEGIRICRQGFPNRLLYADFRQRYRILNPSAIPDDTFVDSRKATEKLLGSLDIDHTQYQFGHTKVFFKAGLLGILEELRDQRLAKVLTLLQARSRGRLMRLEYQRMLGGRDALFTIQWNIRAFNAVKNWSWMKLFFKMKPLLRSAQAEEELAALRAELRGLRGALATAEAKRQELEETQVSVTQEKNDLALQLQAEQDNLADAEERCHLLIKSKVQLEAKVKELNERLEDEEEVNADLAARRRKLEDECTELKKDIDDLELTLAKAEKEKQATENKVKNLTEEMAALDESVVRLTKEKKALQEAHQQALGDLQAEEDRVSALAKAKIRLEQQVEDLECSLEQEKKLRMDTERAKRKLEGDLKLTQETVTDTTQDKQQLEEKLKKKDSELSQLSLRVEDEQLLGAQLQKKIKELQARAEELEEELEAERAARARVEKQRAEAARELEELSERLEEAGGASAGQREGCRKREAELGRLRRELEETVLRHEATVAALRRKQADSAAELSEQVDSLQRIRQKLEKEKSELRMEVDDLGASVETLARGKASAEKLCRTYEDQLSEAKIKVEELQRQLTDASTQRGRLQTENGELGRLLEEKESMISQLSRGKTSAAQSLEELRRQLEEESKAKGALAHAVQALRHDCDLLREQHEEESEAQAELQRLLSKANAEVAQWRSKYEADAIQRTEELEEAKKKLALRLQEAEEGVEAANAKCSSLEKAKLRLQTESEDVTLELERATSAAAALDKKQRHLERALEERRRQEEEMQRELEAAQREARGLGTELFRLRHSHEEALEALETLKRENKNLQEEISDLTDQVSLSGKSIQELEKAKKALEGEKSELQAALEEAEGALELEETKTLRIQLELSQVKAEVDRKLAEKDEECTNLRRNHQRAVESLQASLDAETRARNEALRLKKKMEGDLNDLELQLGHATRQAMEAQAATRLLQAQLKEEQAGRDEEQRLAAELREQGQALERRAALLASELEELRAALEQGERSRRLAEQELLEATERLNLLHSQNTGLLNQKKKLEVDLAQLSGEVEEAAQERREAEEKAKKAITDAAMMAEELKKEQDTSAHLERMKKTLEQTVRELQARLEEAEQAALRGGKKQVQKLEAKVRELEAELDAEQKKHAEALKGVRKHERRVKELAYQTEEDRKNLARMQDLVDKLQSKVKSYKRQFEEAEQQASTNLAKYRKAQHELDDAEERADMAETQANKLRARSRDALGPKVRWPLCWVDTGETRTGDLGQPLSHLLAQGVRDSSSDEGYPVLLLSFHLPLHRALEPQINTTSAAAAAVLSLFLAARGAGNRTSWTKSGPRQSLK

Target ORF information:

RefSeq Version	XM_008762339.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus myosin heavy chain 7B (Myh7b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008762339.2

ORF Insert Sequence:

ATGATGGATA TGAATGAACT TGGAGAATCA GCCTGCTACC TCCGCCAGGG ATACCAGGAA 
ATGATGAAGG TGCACACTGT CCCATGGGAT GGCAAGAAAC GGGTCTGGGT GCCTGATGAA 
CAGGATGCCT ATGTCGAAGC TGAGGTCAAG ACTGAAGCTA CTGGAGGCCG AGTCACTGTG 
GAGACCAAAG ACCAGAAGGT GCTGACAGTG CGTGAAACGG AAATGCAGCC CATGAACCCA 
CCCCGCTTCG ACTTACTGGA GGACATGGCC ATGATGACGC ACCTCAACGA GGCTGCAGTG 
CTGCACAACC TGCGCCAGCG TTATGCTCGC TGGATGATCT ACACATACTC GGGCCTCTTC 
TGTGTCACCA TTAACCCATA CAAATGGCTC CCGGTCTACA CAGCTACTGT GGTGGCCGCC 
TACAAGGGCA AGCGCCGCTC GGAGGCCCCG CCTCATATAT ATGCAGTGGC AGATAATGCC 
TACAATGACA TGCTCCGCAA CAGAGAGAAC CAGTCCATGC TGATCACCGG AGAGTCGGGG 
GCCGGTAAGA CGGTTAACAC CAAGCGGGTC ATTCAGTATT TTGCCATCGT CGCTGCCCTG 
GGAGACGGGC CGGGCAAGAA GGCACAATTT CTGGCAACAA AAACCGGGGG CACCCTTGAG 
GATCAAATCA TCGAGGCTAA CCCTGCCATG GAGGCTTTTG GTAACGCCAA GACTCTGAGG 
AACGACAACT CCTCCCGTTT TGGCAAGTTC ATCCGCATCC ACTTTGGTCC CACTGGGAAG 
CTGGCCTCTG CGGACATTGA CAGCTATCTC CTGGAAAAGT CTCGAGTGAT CTTCCAGCTG 
CCCGGTGAGC GTGGTTACCA TGTCTACTAC CAAATCCTCT CCGGGAAGAA GCCAGAGCTG 
CAGGACATGC TGCTCCTCTC CATGAACCCC TACGACTACC ACTTCTGCAG TCAGGGGGTC 
ACCACCGTGG ATAACATGAA CGACGGGGAG GAGCTTATCG CCACTGATCA TGCAATGGAC 
ATCCTGGGCT TCAGCGTGGA TGAGAAGTGC GCATGCTATA AGATCGTGGG CGCCCTCCTG 
CACTTTGGCA ACATGAAGTT CAAACAGAAG CAGAGAGAGG AACAGGCTGA GGCAGACGGC 
ACTGAGAGTG CCGACAAGGC TGCCTACCTG ATGGGAGTCA GCAGTGGAGA CCTCCTCAAA 
GGTCTTCTGC ACCCCCGGGT TCGTGTGGGA AACGAGTATG TGACCAAGGG CCAGAGTGTG 
GAGCAGGTGG TATTTGCTGT GGGGGCCCTG GCCAAGGCTA CCTATGACCG GTTGTTCCGA 
TGGCTGGTAT CTCGAATCAA TCAGACCCTG GACACAAAGC TCCCCCGTCA GTTCTTCATT 
GGGGTCCTGG ATATCGCTGG TTTTGAGATC TTTGAGTTTA ACAGCTTCGA GCAACTGTGT 
ATCAACTTCA CCAACGAGAA ACTGCAGCAG TTCTTCAACC AGCACATGTT CGTGCTGGAG 
CAGGAGGAGT ACAAACGGGA GGGCATTGAC TGGGTCTTCA TCGACTTCGG CCTTGACCTG 
CAGCCCTGCA TCGACCTCAT CGAGAAGCCT CTGGGCATTC TGTCCATCCT GGAGGAGGAG 
TGCATGTTCC CCAAGGCTTC AGACGCAAGC TTCAGGGCCA AGCTCTATGA CAATCACTCT 
GGGAAGTCAC CTAATTTCCA GCAGCCTCGC CCAGACAAGA AACGCAAGTA CCAGGCTCAC 
TTCGAGGTGG TCCACTACGC AGGCGTGGTA CCTTATAGCA TTGTGGGCTG GCTGGAGAAA 
AACAAGGACC CCCTCAATGA GACCGTGGTC CCCATCTTCC AAAAGTCACA GAATAGGCTC 
TTGGCCACCC TCTATGAGAA CTATGCAGGT TCTTGCTCCA CCGAGCCCCC CAAGTCTGGG 
GTGAAAGAGA AACGAAAGAA AGCAGCATCA TTTCAGACAG TGTCTCAGCT TCACAAGGAG 
AACCTCAACA AGCTTATGAC CAACCTGCGG GCCACACAGC CCCACTTCGT CCGTTGTATC 
GTCCCCAACG AGAACAAGAC TCCAGGGGTC ATGGATTCCT TCTTGGTGCT ACACCAGCTA 
CGCTGTAACG GGGTCCTAGA GGGGATCCGG ATCTGCCGCC AAGGGTTCCC CAACAGACTG 
CTGTATGCTG ACTTCAGGCA GCGGTACCGA ATCCTGAACC CCAGTGCCAT CCCAGATGAC 
ACCTTTGTGG ACAGCAGGAA GGCCACAGAG AAGCTGCTGG GCTCTCTGGA CATTGACCAC 
ACCCAGTATC AGTTTGGCCA CACCAAGGTG TTCTTCAAGG CTGGGCTTCT AGGCATCCTG 
GAGGAGCTCC GGGACCAGCG TCTGGCTAAG GTGCTGACAC TGCTGCAGGC TCGGAGCCGG 
GGCCGCCTCA TGCGTCTGGA GTACCAGCGC ATGCTAGGAG GAAGGGATGC CCTGTTCACC 
ATCCAGTGGA ACATCCGGGC CTTCAATGCC GTCAAGAACT GGTCCTGGAT GAAGCTCTTC 
TTCAAGATGA AGCCACTGCT GCGCTCAGCA CAGGCAGAGG AAGAGCTGGC AGCACTTCGG 
GCAGAACTGC GGGGGCTGCG GGGGGCCCTG GCTACGGCTG AGGCCAAGCG TCAGGAGCTG 
GAGGAGACTC AAGTCAGTGT CACTCAGGAG AAGAATGACC TGGCCCTGCA GCTGCAGGCA 
GAGCAGGACA ACCTAGCGGA TGCGGAGGAG CGCTGCCACT TGCTCATCAA GTCCAAGGTG 
CAGCTCGAGG CGAAGGTGAA GGAGCTGAAC GAGCGGCTGG AGGATGAGGA GGAAGTGAAC 
GCTGACCTGG CCGCACGCAG GCGCAAGCTG GAGGATGAGT GCACGGAGCT CAAGAAGGAC 
ATTGATGACC TGGAGCTGAC CCTGGCCAAG GCTGAGAAGG AGAAACAAGC CACCGAGAAT 
AAGGTGAAGA ACCTGACAGA GGAGATGGCT GCCCTGGACG AGTCCGTGGT CCGCCTGACT 
AAGGAGAAGA AGGCGTTGCA GGAGGCTCAC CAGCAGGCTC TGGGTGACCT GCAGGCTGAA 
GAGGACCGTG TGAGTGCGCT GGCCAAGGCC AAGATCCGGT TGGAGCAGCA AGTAGAGGAC 
CTGGAGTGCT CCCTGGAGCA AGAGAAGAAG CTTCGCATGG ATACAGAACG TGCCAAGCGC 
AAGCTCGAAG GAGACCTGAA GCTGACGCAG GAGACAGTGA CAGACACCAC ACAAGACAAG 
CAGCAGCTGG AGGAGAAGTT GAAGAAGAAG GATTCAGAGC TGAGTCAGCT GAGCCTGCGG 
GTGGAGGATG AGCAGCTCCT GGGGGCGCAG TTGCAGAAGA AGATCAAGGA GCTACAGGCG 
AGGGCTGAGG AGCTGGAGGA GGAGCTGGAG GCGGAGCGAG CAGCCCGTGC CCGTGTGGAA 
AAGCAGCGAG CTGAGGCAGC CCGAGAGCTG GAGGAGCTGA GTGAGAGGCT AGAGGAGGCT 
GGTGGAGCCT CTGCCGGGCA GCGTGAGGGT TGCCGCAAGC GTGAGGCTGA GTTGGGCCGA 
CTGCGGCGGG AGCTGGAGGA GACAGTTCTG AGACACGAGG CCACTGTGGC TGCCCTGCGC 
CGCAAGCAGG CTGATAGCGC CGCAGAGTTG AGCGAGCAGG TAGACAGCCT CCAGCGGATC 
CGGCAGAAAC TGGAGAAGGA AAAGAGTGAG CTGCGCATGG AGGTGGATGA TCTGGGGGCC 
AGCGTGGAAA CTCTGGCCCG TGGCAAGGCC AGTGCAGAGA AGCTGTGCCG GACCTATGAG 
GACCAGCTCA GCGAGGCCAA GATCAAAGTG GAGGAGCTGC AGCGGCAGCT GACTGACGCC 
AGCACCCAGC GGGGGCGGTT ACAAACGGAG AACGGGGAGC TGGGCCGCCT GCTTGAGGAG 
AAGGAGTCTA TGATCAGCCA GCTGAGCCGC GGGAAGACCT CAGCCGCTCA GAGTCTGGAG 
GAACTTCGGC GGCAGTTGGA GGAAGAAAGC AAGGCCAAGG GTGCGCTGGC TCACGCAGTG 
CAGGCTCTGC GGCACGACTG CGACCTCCTG CGAGAGCAGC ATGAGGAAGA GTCCGAGGCG 
CAGGCTGAGC TGCAGCGGCT GCTGTCCAAA GCCAACGCTG AGGTGGCACA GTGGAGGAGC 
AAGTATGAGG CGGACGCCAT CCAAAGGACG GAGGAGCTGG AGGAGGCCAA GAAGAAGCTG 
GCCCTGAGGC TGCAGGAGGC AGAAGAAGGC GTAGAGGCTG CGAATGCCAA GTGCTCCTCA 
CTGGAGAAGG CCAAGCTGCG GCTGCAGACA GAGTCCGAGG ACGTGACCTT GGAGCTGGAG 
CGGGCCACCT CAGCCGCTGC TGCACTGGAT AAGAAGCAGC GGCACTTGGA GCGGGCCCTG 
GAGGAACGGA GGCGGCAGGA GGAGGAGATG CAGCGGGAAC TGGAGGCGGC GCAGAGGGAG 
GCTCGAGGTC TGGGCACCGA GCTCTTTCGA TTGCGGCACA GCCATGAGGA GGCGCTTGAG 
GCTCTGGAGA CACTCAAGCG GGAGAACAAG AATCTGCAGG AGGAGATCAG CGACCTTACA 
GACCAGGTCA GCCTCAGCGG GAAGAGCATC CAGGAACTGG AGAAGGCCAA GAAGGCGCTG 
GAAGGGGAGA AGAGTGAGCT GCAGGCTGCG CTGGAGGAGG CTGAGGGGGC GCTGGAGCTG 
GAAGAGACAA AGACCCTGCG GATCCAGTTG GAGTTGTCCC AGGTCAAGGC GGAAGTGGAC 
CGAAAGCTGG CGGAGAAGGA TGAGGAGTGT ACTAACCTGA GGCGCAACCA CCAGCGGGCT 
GTGGAGTCCT TGCAGGCCTC CCTGGATGCC GAGACTAGGG CCCGCAATGA AGCACTGCGT 
CTCAAGAAGA AGATGGAGGG GGACCTCAAT GACCTGGAGT TACAGCTGGG CCACGCAACC 
CGCCAGGCCA TGGAGGCACA GGCAGCCACG AGGCTGCTTC AGGCCCAACT CAAGGAGGAG 
CAAGCAGGGC GTGATGAGGA GCAGAGGCTG GCTGCCGAGC TCCGCGAGCA GGGCCAGGCC 
CTCGAGCGCA GGGCTGCACT GCTGGCATCT GAGCTGGAAG AGCTTCGGGC CGCCCTGGAA 
CAGGGCGAGC GCAGCCGAAG GCTGGCTGAA CAGGAGCTGT TGGAGGCCAC AGAGCGCCTC 
AACCTACTGC ATTCACAGAA CACAGGCCTC CTGAACCAGA AAAAGAAGCT GGAGGTGGAC 
TTGGCCCAGC TGAGTGGGGA GGTGGAGGAG GCTGCGCAGG AGAGGCGGGA AGCTGAGGAG 
AAGGCCAAAA AGGCCATCAC GGATGCAGCC ATGATGGCTG AGGAGCTGAA AAAGGAGCAG 
GACACAAGTG CACACCTCGA ACGGATGAAG AAGACCCTGG AACAGACAGT GCGGGAGCTG 
CAGGCCCGCC TTGAGGAAGC AGAGCAGGCC GCACTCCGGG GTGGGAAGAA GCAAGTGCAG 
AAGCTGGAAG CCAAGGTGCG GGAACTGGAG GCAGAGCTTG ACGCAGAGCA GAAGAAGCAC 
GCTGAGGCCC TCAAGGGTGT GCGCAAACAT GAGCGCAGGG TCAAGGAGCT TGCGTACCAG 
ACTGAGGAAG ACAGGAAGAA CCTGGCTCGC ATGCAGGACC TAGTGGACAA GCTGCAGAGC 
AAGGTCAAGA GCTACAAGCG TCAGTTTGAG GAGGCGGAGC AGCAGGCCAG TACCAACCTG 
GCTAAGTACC GCAAGGCCCA GCACGAGTTA GACGACGCTG AGGAGCGGGC AGACATGGCA 
GAGACCCAGG CCAACAAGCT GCGGGCAAGG TCCAGGGATG CCCTGGGTCC CAAGGTAAGG 
TGGCCACTGT GTTGGGTGGA CACTGGGGAG ACACGCACGG GTGACCTAGG TCAGCCTCTT 
TCTCATCTCT TAGCACAAGG AGTGAGAGAC TCCAGCTCTG ACGAGGGATA CCCCGTGCTC 
CTGTTGTCTT TCCACCTGCC ACTCCACAGA GCTCTGGAAC CCCAAATAAA CACCACATCT 
GCAGCAGCAG CCGCTGTCCT GTCCTTATTT CTTGCGGCTA GAGGAGCTGG GAACAGAACC 
TCATGGACAA AGTCAGGCCC ACGTCAATCT TTAAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Myh7b ( XM_017591782.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017447271.1 Rattus norvegicus myosin heavy chain 7B (Myh7b), transcript variant X3, mRNA.

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CloneID	ORa47041
Clone ID Related Accession (Same CDS sequence)	XM_008762340.2 , XM_008762339.2 , XM_017591782.1
Accession Version	XM_017591782.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6099bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	myosin-7B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005102.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGATA TGAATGAACT TGGAGAATCA GCCTGCTACC TCCGCCAGGG ATACCAGGAA 
ATGATGAAGG TGCACACTGT CCCATGGGAT GGCAAGAAAC GGGTCTGGGT GCCTGATGAA 
CAGGATGCCT ATGTCGAAGC TGAGGTCAAG ACTGAAGCTA CTGGAGGCCG AGTCACTGTG 
GAGACCAAAG ACCAGAAGGT GCTGACAGTG CGTGAAACGG AAATGCAGCC CATGAACCCA 
CCCCGCTTCG ACTTACTGGA GGACATGGCC ATGATGACGC ACCTCAACGA GGCTGCAGTG 
CTGCACAACC TGCGCCAGCG TTATGCTCGC TGGATGATCT ACACATACTC GGGCCTCTTC 
TGTGTCACCA TTAACCCATA CAAATGGCTC CCGGTCTACA CAGCTACTGT GGTGGCCGCC 
TACAAGGGCA AGCGCCGCTC GGAGGCCCCG CCTCATATAT ATGCAGTGGC AGATAATGCC 
TACAATGACA TGCTCCGCAA CAGAGAGAAC CAGTCCATGC TGATCACCGG AGAGTCGGGG 
GCCGGTAAGA CGGTTAACAC CAAGCGGGTC ATTCAGTATT TTGCCATCGT CGCTGCCCTG 
GGAGACGGGC CGGGCAAGAA GGCACAATTT CTGGCAACAA AAACCGGGGG CACCCTTGAG 
GATCAAATCA TCGAGGCTAA CCCTGCCATG GAGGCTTTTG GTAACGCCAA GACTCTGAGG 
AACGACAACT CCTCCCGTTT TGGCAAGTTC ATCCGCATCC ACTTTGGTCC CACTGGGAAG 
CTGGCCTCTG CGGACATTGA CAGCTATCTC CTGGAAAAGT CTCGAGTGAT CTTCCAGCTG 
CCCGGTGAGC GTGGTTACCA TGTCTACTAC CAAATCCTCT CCGGGAAGAA GCCAGAGCTG 
CAGGACATGC TGCTCCTCTC CATGAACCCC TACGACTACC ACTTCTGCAG TCAGGGGGTC 
ACCACCGTGG ATAACATGAA CGACGGGGAG GAGCTTATCG CCACTGATCA TGCAATGGAC 
ATCCTGGGCT TCAGCGTGGA TGAGAAGTGC GCATGCTATA AGATCGTGGG CGCCCTCCTG 
CACTTTGGCA ACATGAAGTT CAAACAGAAG CAGAGAGAGG AACAGGCTGA GGCAGACGGC 
ACTGAGAGTG CCGACAAGGC TGCCTACCTG ATGGGAGTCA GCAGTGGAGA CCTCCTCAAA 
GGTCTTCTGC ACCCCCGGGT TCGTGTGGGA AACGAGTATG TGACCAAGGG CCAGAGTGTG 
GAGCAGGTGG TATTTGCTGT GGGGGCCCTG GCCAAGGCTA CCTATGACCG GTTGTTCCGA 
TGGCTGGTAT CTCGAATCAA TCAGACCCTG GACACAAAGC TCCCCCGTCA GTTCTTCATT 
GGGGTCCTGG ATATCGCTGG TTTTGAGATC TTTGAGTTTA ACAGCTTCGA GCAACTGTGT 
ATCAACTTCA CCAACGAGAA ACTGCAGCAG TTCTTCAACC AGCACATGTT CGTGCTGGAG 
CAGGAGGAGT ACAAACGGGA GGGCATTGAC TGGGTCTTCA TCGACTTCGG CCTTGACCTG 
CAGCCCTGCA TCGACCTCAT CGAGAAGCCT CTGGGCATTC TGTCCATCCT GGAGGAGGAG 
TGCATGTTCC CCAAGGCTTC AGACGCAAGC TTCAGGGCCA AGCTCTATGA CAATCACTCT 
GGGAAGTCAC CTAATTTCCA GCAGCCTCGC CCAGACAAGA AACGCAAGTA CCAGGCTCAC 
TTCGAGGTGG TCCACTACGC AGGCGTGGTA CCTTATAGCA TTGTGGGCTG GCTGGAGAAA 
AACAAGGACC CCCTCAATGA GACCGTGGTC CCCATCTTCC AAAAGTCACA GAATAGGCTC 
TTGGCCACCC TCTATGAGAA CTATGCAGGT TCTTGCTCCA CCGAGCCCCC CAAGTCTGGG 
GTGAAAGAGA AACGAAAGAA AGCAGCATCA TTTCAGACAG TGTCTCAGCT TCACAAGGAG 
AACCTCAACA AGCTTATGAC CAACCTGCGG GCCACACAGC CCCACTTCGT CCGTTGTATC 
GTCCCCAACG AGAACAAGAC TCCAGGGGTC ATGGATTCCT TCTTGGTGCT ACACCAGCTA 
CGCTGTAACG GGGTCCTAGA GGGGATCCGG ATCTGCCGCC AAGGGTTCCC CAACAGACTG 
CTGTATGCTG ACTTCAGGCA GCGGTACCGA ATCCTGAACC CCAGTGCCAT CCCAGATGAC 
ACCTTTGTGG ACAGCAGGAA GGCCACAGAG AAGCTGCTGG GCTCTCTGGA CATTGACCAC 
ACCCAGTATC AGTTTGGCCA CACCAAGGTG TTCTTCAAGG CTGGGCTTCT AGGCATCCTG 
GAGGAGCTCC GGGACCAGCG TCTGGCTAAG GTGCTGACAC TGCTGCAGGC TCGGAGCCGG 
GGCCGCCTCA TGCGTCTGGA GTACCAGCGC ATGCTAGGAG GAAGGGATGC CCTGTTCACC 
ATCCAGTGGA ACATCCGGGC CTTCAATGCC GTCAAGAACT GGTCCTGGAT GAAGCTCTTC 
TTCAAGATGA AGCCACTGCT GCGCTCAGCA CAGGCAGAGG AAGAGCTGGC AGCACTTCGG 
GCAGAACTGC GGGGGCTGCG GGGGGCCCTG GCTACGGCTG AGGCCAAGCG TCAGGAGCTG 
GAGGAGACTC AAGTCAGTGT CACTCAGGAG AAGAATGACC TGGCCCTGCA GCTGCAGGCA 
GAGCAGGACA ACCTAGCGGA TGCGGAGGAG CGCTGCCACT TGCTCATCAA GTCCAAGGTG 
CAGCTCGAGG CGAAGGTGAA GGAGCTGAAC GAGCGGCTGG AGGATGAGGA GGAAGTGAAC 
GCTGACCTGG CCGCACGCAG GCGCAAGCTG GAGGATGAGT GCACGGAGCT CAAGAAGGAC 
ATTGATGACC TGGAGCTGAC CCTGGCCAAG GCTGAGAAGG AGAAACAAGC CACCGAGAAT 
AAGGTGAAGA ACCTGACAGA GGAGATGGCT GCCCTGGACG AGTCCGTGGT CCGCCTGACT 
AAGGAGAAGA AGGCGTTGCA GGAGGCTCAC CAGCAGGCTC TGGGTGACCT GCAGGCTGAA 
GAGGACCGTG TGAGTGCGCT GGCCAAGGCC AAGATCCGGT TGGAGCAGCA AGTAGAGGAC 
CTGGAGTGCT CCCTGGAGCA AGAGAAGAAG CTTCGCATGG ATACAGAACG TGCCAAGCGC 
AAGCTCGAAG GAGACCTGAA GCTGACGCAG GAGACAGTGA CAGACACCAC ACAAGACAAG 
CAGCAGCTGG AGGAGAAGTT GAAGAAGAAG GATTCAGAGC TGAGTCAGCT GAGCCTGCGG 
GTGGAGGATG AGCAGCTCCT GGGGGCGCAG TTGCAGAAGA AGATCAAGGA GCTACAGGCG 
AGGGCTGAGG AGCTGGAGGA GGAGCTGGAG GCGGAGCGAG CAGCCCGTGC CCGTGTGGAA 
AAGCAGCGAG CTGAGGCAGC CCGAGAGCTG GAGGAGCTGA GTGAGAGGCT AGAGGAGGCT 
GGTGGAGCCT CTGCCGGGCA GCGTGAGGGT TGCCGCAAGC GTGAGGCTGA GTTGGGCCGA 
CTGCGGCGGG AGCTGGAGGA GACAGTTCTG AGACACGAGG CCACTGTGGC TGCCCTGCGC 
CGCAAGCAGG CTGATAGCGC CGCAGAGTTG AGCGAGCAGG TAGACAGCCT CCAGCGGATC 
CGGCAGAAAC TGGAGAAGGA AAAGAGTGAG CTGCGCATGG AGGTGGATGA TCTGGGGGCC 
AGCGTGGAAA CTCTGGCCCG TGGCAAGGCC AGTGCAGAGA AGCTGTGCCG GACCTATGAG 
GACCAGCTCA GCGAGGCCAA GATCAAAGTG GAGGAGCTGC AGCGGCAGCT GACTGACGCC 
AGCACCCAGC GGGGGCGGTT ACAAACGGAG AACGGGGAGC TGGGCCGCCT GCTTGAGGAG 
AAGGAGTCTA TGATCAGCCA GCTGAGCCGC GGGAAGACCT CAGCCGCTCA GAGTCTGGAG 
GAACTTCGGC GGCAGTTGGA GGAAGAAAGC AAGGCCAAGG GTGCGCTGGC TCACGCAGTG 
CAGGCTCTGC GGCACGACTG CGACCTCCTG CGAGAGCAGC ATGAGGAAGA GTCCGAGGCG 
CAGGCTGAGC TGCAGCGGCT GCTGTCCAAA GCCAACGCTG AGGTGGCACA GTGGAGGAGC 
AAGTATGAGG CGGACGCCAT CCAAAGGACG GAGGAGCTGG AGGAGGCCAA GAAGAAGCTG 
GCCCTGAGGC TGCAGGAGGC AGAAGAAGGC GTAGAGGCTG CGAATGCCAA GTGCTCCTCA 
CTGGAGAAGG CCAAGCTGCG GCTGCAGACA GAGTCCGAGG ACGTGACCTT GGAGCTGGAG 
CGGGCCACCT CAGCCGCTGC TGCACTGGAT AAGAAGCAGC GGCACTTGGA GCGGGCCCTG 
GAGGAACGGA GGCGGCAGGA GGAGGAGATG CAGCGGGAAC TGGAGGCGGC GCAGAGGGAG 
GCTCGAGGTC TGGGCACCGA GCTCTTTCGA TTGCGGCACA GCCATGAGGA GGCGCTTGAG 
GCTCTGGAGA CACTCAAGCG GGAGAACAAG AATCTGCAGG AGGAGATCAG CGACCTTACA 
GACCAGGTCA GCCTCAGCGG GAAGAGCATC CAGGAACTGG AGAAGGCCAA GAAGGCGCTG 
GAAGGGGAGA AGAGTGAGCT GCAGGCTGCG CTGGAGGAGG CTGAGGGGGC GCTGGAGCTG 
GAAGAGACAA AGACCCTGCG GATCCAGTTG GAGTTGTCCC AGGTCAAGGC GGAAGTGGAC 
CGAAAGCTGG CGGAGAAGGA TGAGGAGTGT ACTAACCTGA GGCGCAACCA CCAGCGGGCT 
GTGGAGTCCT TGCAGGCCTC CCTGGATGCC GAGACTAGGG CCCGCAATGA AGCACTGCGT 
CTCAAGAAGA AGATGGAGGG GGACCTCAAT GACCTGGAGT TACAGCTGGG CCACGCAACC 
CGCCAGGCCA TGGAGGCACA GGCAGCCACG AGGCTGCTTC AGGCCCAACT CAAGGAGGAG 
CAAGCAGGGC GTGATGAGGA GCAGAGGCTG GCTGCCGAGC TCCGCGAGCA GGGCCAGGCC 
CTCGAGCGCA GGGCTGCACT GCTGGCATCT GAGCTGGAAG AGCTTCGGGC CGCCCTGGAA 
CAGGGCGAGC GCAGCCGAAG GCTGGCTGAA CAGGAGCTGT TGGAGGCCAC AGAGCGCCTC 
AACCTACTGC ATTCACAGAA CACAGGCCTC CTGAACCAGA AAAAGAAGCT GGAGGTGGAC 
TTGGCCCAGC TGAGTGGGGA GGTGGAGGAG GCTGCGCAGG AGAGGCGGGA AGCTGAGGAG 
AAGGCCAAAA AGGCCATCAC GGATGCAGCC ATGATGGCTG AGGAGCTGAA AAAGGAGCAG 
GACACAAGTG CACACCTCGA ACGGATGAAG AAGACCCTGG AACAGACAGT GCGGGAGCTG 
CAGGCCCGCC TTGAGGAAGC AGAGCAGGCC GCACTCCGGG GTGGGAAGAA GCAAGTGCAG 
AAGCTGGAAG CCAAGGTGCG GGAACTGGAG GCAGAGCTTG ACGCAGAGCA GAAGAAGCAC 
GCTGAGGCCC TCAAGGGTGT GCGCAAACAT GAGCGCAGGG TCAAGGAGCT TGCGTACCAG 
ACTGAGGAAG ACAGGAAGAA CCTGGCTCGC ATGCAGGACC TAGTGGACAA GCTGCAGAGC 
AAGGTCAAGA GCTACAAGCG TCAGTTTGAG GAGGCGGAGC AGCAGGCCAG TACCAACCTG 
GCTAAGTACC GCAAGGCCCA GCACGAGTTA GACGACGCTG AGGAGCGGGC AGACATGGCA 
GAGACCCAGG CCAACAAGCT GCGGGCAAGG TCCAGGGATG CCCTGGGTCC CAAGGTAAGG 
TGGCCACTGT GTTGGGTGGA CACTGGGGAG ACACGCACGG GTGACCTAGG TCAGCCTCTT 
TCTCATCTCT TAGCACAAGG AGTGAGAGAC TCCAGCTCTG ACGAGGGATA CCCCGTGCTC 
CTGTTGTCTT TCCACCTGCC ACTCCACAGA GCTCTGGAAC CCCAAATAAA CACCACATCT 
GCAGCAGCAG CCGCTGTCCT GTCCTTATTT CTTGCGGCTA GAGGAGCTGG GAACAGAACC 
TCATGGACAA AGTCAGGCCC ACGTCAATCT TTAAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017447271.1
CDS	713..6811
Translation	MMDMNELGESACYLRQGYQEMMKVHTVPWDGKKRVWVPDEQDAYVEAEVKTEATGGRVTVETKDQKVLTVRETEMQPMNPPRFDLLEDMAMMTHLNEAAVLHNLRQRYARWMIYTYSGLFCVTINPYKWLPVYTATVVAAYKGKRRSEAPPHIYAVADNAYNDMLRNRENQSMLITGESGAGKTVNTKRVIQYFAIVAALGDGPGKKAQFLATKTGGTLEDQIIEANPAMEAFGNAKTLRNDNSSRFGKFIRIHFGPTGKLASADIDSYLLEKSRVIFQLPGERGYHVYYQILSGKKPELQDMLLLSMNPYDYHFCSQGVTTVDNMNDGEELIATDHAMDILGFSVDEKCACYKIVGALLHFGNMKFKQKQREEQAEADGTESADKAAYLMGVSSGDLLKGLLHPRVRVGNEYVTKGQSVEQVVFAVGALAKATYDRLFRWLVSRINQTLDTKLPRQFFIGVLDIAGFEIFEFNSFEQLCINFTNEKLQQFFNQHMFVLEQEEYKREGIDWVFIDFGLDLQPCIDLIEKPLGILSILEEECMFPKASDASFRAKLYDNHSGKSPNFQQPRPDKKRKYQAHFEVVHYAGVVPYSIVGWLEKNKDPLNETVVPIFQKSQNRLLATLYENYAGSCSTEPPKSGVKEKRKKAASFQTVSQLHKENLNKLMTNLRATQPHFVRCIVPNENKTPGVMDSFLVLHQLRCNGVLEGIRICRQGFPNRLLYADFRQRYRILNPSAIPDDTFVDSRKATEKLLGSLDIDHTQYQFGHTKVFFKAGLLGILEELRDQRLAKVLTLLQARSRGRLMRLEYQRMLGGRDALFTIQWNIRAFNAVKNWSWMKLFFKMKPLLRSAQAEEELAALRAELRGLRGALATAEAKRQELEETQVSVTQEKNDLALQLQAEQDNLADAEERCHLLIKSKVQLEAKVKELNERLEDEEEVNADLAARRRKLEDECTELKKDIDDLELTLAKAEKEKQATENKVKNLTEEMAALDESVVRLTKEKKALQEAHQQALGDLQAEEDRVSALAKAKIRLEQQVEDLECSLEQEKKLRMDTERAKRKLEGDLKLTQETVTDTTQDKQQLEEKLKKKDSELSQLSLRVEDEQLLGAQLQKKIKELQARAEELEEELEAERAARARVEKQRAEAARELEELSERLEEAGGASAGQREGCRKREAELGRLRRELEETVLRHEATVAALRRKQADSAAELSEQVDSLQRIRQKLEKEKSELRMEVDDLGASVETLARGKASAEKLCRTYEDQLSEAKIKVEELQRQLTDASTQRGRLQTENGELGRLLEEKESMISQLSRGKTSAAQSLEELRRQLEEESKAKGALAHAVQALRHDCDLLREQHEEESEAQAELQRLLSKANAEVAQWRSKYEADAIQRTEELEEAKKKLALRLQEAEEGVEAANAKCSSLEKAKLRLQTESEDVTLELERATSAAAALDKKQRHLERALEERRRQEEEMQRELEAAQREARGLGTELFRLRHSHEEALEALETLKRENKNLQEEISDLTDQVSLSGKSIQELEKAKKALEGEKSELQAALEEAEGALELEETKTLRIQLELSQVKAEVDRKLAEKDEECTNLRRNHQRAVESLQASLDAETRARNEALRLKKKMEGDLNDLELQLGHATRQAMEAQAATRLLQAQLKEEQAGRDEEQRLAAELREQGQALERRAALLASELEELRAALEQGERSRRLAEQELLEATERLNLLHSQNTGLLNQKKKLEVDLAQLSGEVEEAAQERREAEEKAKKAITDAAMMAEELKKEQDTSAHLERMKKTLEQTVRELQARLEEAEQAALRGGKKQVQKLEAKVRELEAELDAEQKKHAEALKGVRKHERRVKELAYQTEEDRKNLARMQDLVDKLQSKVKSYKRQFEEAEQQASTNLAKYRKAQHELDDAEERADMAETQANKLRARSRDALGPKVRWPLCWVDTGETRTGDLGQPLSHLLAQGVRDSSSDEGYPVLLLSFHLPLHRALEPQINTTSAAAAAVLSLFLAARGAGNRTSWTKSGPRQSLK

Target ORF information:

RefSeq Version	XM_017591782.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus myosin heavy chain 7B (Myh7b), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017591782.1

ORF Insert Sequence:

ATGATGGATA TGAATGAACT TGGAGAATCA GCCTGCTACC TCCGCCAGGG ATACCAGGAA 
ATGATGAAGG TGCACACTGT CCCATGGGAT GGCAAGAAAC GGGTCTGGGT GCCTGATGAA 
CAGGATGCCT ATGTCGAAGC TGAGGTCAAG ACTGAAGCTA CTGGAGGCCG AGTCACTGTG 
GAGACCAAAG ACCAGAAGGT GCTGACAGTG CGTGAAACGG AAATGCAGCC CATGAACCCA 
CCCCGCTTCG ACTTACTGGA GGACATGGCC ATGATGACGC ACCTCAACGA GGCTGCAGTG 
CTGCACAACC TGCGCCAGCG TTATGCTCGC TGGATGATCT ACACATACTC GGGCCTCTTC 
TGTGTCACCA TTAACCCATA CAAATGGCTC CCGGTCTACA CAGCTACTGT GGTGGCCGCC 
TACAAGGGCA AGCGCCGCTC GGAGGCCCCG CCTCATATAT ATGCAGTGGC AGATAATGCC 
TACAATGACA TGCTCCGCAA CAGAGAGAAC CAGTCCATGC TGATCACCGG AGAGTCGGGG 
GCCGGTAAGA CGGTTAACAC CAAGCGGGTC ATTCAGTATT TTGCCATCGT CGCTGCCCTG 
GGAGACGGGC CGGGCAAGAA GGCACAATTT CTGGCAACAA AAACCGGGGG CACCCTTGAG 
GATCAAATCA TCGAGGCTAA CCCTGCCATG GAGGCTTTTG GTAACGCCAA GACTCTGAGG 
AACGACAACT CCTCCCGTTT TGGCAAGTTC ATCCGCATCC ACTTTGGTCC CACTGGGAAG 
CTGGCCTCTG CGGACATTGA CAGCTATCTC CTGGAAAAGT CTCGAGTGAT CTTCCAGCTG 
CCCGGTGAGC GTGGTTACCA TGTCTACTAC CAAATCCTCT CCGGGAAGAA GCCAGAGCTG 
CAGGACATGC TGCTCCTCTC CATGAACCCC TACGACTACC ACTTCTGCAG TCAGGGGGTC 
ACCACCGTGG ATAACATGAA CGACGGGGAG GAGCTTATCG CCACTGATCA TGCAATGGAC 
ATCCTGGGCT TCAGCGTGGA TGAGAAGTGC GCATGCTATA AGATCGTGGG CGCCCTCCTG 
CACTTTGGCA ACATGAAGTT CAAACAGAAG CAGAGAGAGG AACAGGCTGA GGCAGACGGC 
ACTGAGAGTG CCGACAAGGC TGCCTACCTG ATGGGAGTCA GCAGTGGAGA CCTCCTCAAA 
GGTCTTCTGC ACCCCCGGGT TCGTGTGGGA AACGAGTATG TGACCAAGGG CCAGAGTGTG 
GAGCAGGTGG TATTTGCTGT GGGGGCCCTG GCCAAGGCTA CCTATGACCG GTTGTTCCGA 
TGGCTGGTAT CTCGAATCAA TCAGACCCTG GACACAAAGC TCCCCCGTCA GTTCTTCATT 
GGGGTCCTGG ATATCGCTGG TTTTGAGATC TTTGAGTTTA ACAGCTTCGA GCAACTGTGT 
ATCAACTTCA CCAACGAGAA ACTGCAGCAG TTCTTCAACC AGCACATGTT CGTGCTGGAG 
CAGGAGGAGT ACAAACGGGA GGGCATTGAC TGGGTCTTCA TCGACTTCGG CCTTGACCTG 
CAGCCCTGCA TCGACCTCAT CGAGAAGCCT CTGGGCATTC TGTCCATCCT GGAGGAGGAG 
TGCATGTTCC CCAAGGCTTC AGACGCAAGC TTCAGGGCCA AGCTCTATGA CAATCACTCT 
GGGAAGTCAC CTAATTTCCA GCAGCCTCGC CCAGACAAGA AACGCAAGTA CCAGGCTCAC 
TTCGAGGTGG TCCACTACGC AGGCGTGGTA CCTTATAGCA TTGTGGGCTG GCTGGAGAAA 
AACAAGGACC CCCTCAATGA GACCGTGGTC CCCATCTTCC AAAAGTCACA GAATAGGCTC 
TTGGCCACCC TCTATGAGAA CTATGCAGGT TCTTGCTCCA CCGAGCCCCC CAAGTCTGGG 
GTGAAAGAGA AACGAAAGAA AGCAGCATCA TTTCAGACAG TGTCTCAGCT TCACAAGGAG 
AACCTCAACA AGCTTATGAC CAACCTGCGG GCCACACAGC CCCACTTCGT CCGTTGTATC 
GTCCCCAACG AGAACAAGAC TCCAGGGGTC ATGGATTCCT TCTTGGTGCT ACACCAGCTA 
CGCTGTAACG GGGTCCTAGA GGGGATCCGG ATCTGCCGCC AAGGGTTCCC CAACAGACTG 
CTGTATGCTG ACTTCAGGCA GCGGTACCGA ATCCTGAACC CCAGTGCCAT CCCAGATGAC 
ACCTTTGTGG ACAGCAGGAA GGCCACAGAG AAGCTGCTGG GCTCTCTGGA CATTGACCAC 
ACCCAGTATC AGTTTGGCCA CACCAAGGTG TTCTTCAAGG CTGGGCTTCT AGGCATCCTG 
GAGGAGCTCC GGGACCAGCG TCTGGCTAAG GTGCTGACAC TGCTGCAGGC TCGGAGCCGG 
GGCCGCCTCA TGCGTCTGGA GTACCAGCGC ATGCTAGGAG GAAGGGATGC CCTGTTCACC 
ATCCAGTGGA ACATCCGGGC CTTCAATGCC GTCAAGAACT GGTCCTGGAT GAAGCTCTTC 
TTCAAGATGA AGCCACTGCT GCGCTCAGCA CAGGCAGAGG AAGAGCTGGC AGCACTTCGG 
GCAGAACTGC GGGGGCTGCG GGGGGCCCTG GCTACGGCTG AGGCCAAGCG TCAGGAGCTG 
GAGGAGACTC AAGTCAGTGT CACTCAGGAG AAGAATGACC TGGCCCTGCA GCTGCAGGCA 
GAGCAGGACA ACCTAGCGGA TGCGGAGGAG CGCTGCCACT TGCTCATCAA GTCCAAGGTG 
CAGCTCGAGG CGAAGGTGAA GGAGCTGAAC GAGCGGCTGG AGGATGAGGA GGAAGTGAAC 
GCTGACCTGG CCGCACGCAG GCGCAAGCTG GAGGATGAGT GCACGGAGCT CAAGAAGGAC 
ATTGATGACC TGGAGCTGAC CCTGGCCAAG GCTGAGAAGG AGAAACAAGC CACCGAGAAT 
AAGGTGAAGA ACCTGACAGA GGAGATGGCT GCCCTGGACG AGTCCGTGGT CCGCCTGACT 
AAGGAGAAGA AGGCGTTGCA GGAGGCTCAC CAGCAGGCTC TGGGTGACCT GCAGGCTGAA 
GAGGACCGTG TGAGTGCGCT GGCCAAGGCC AAGATCCGGT TGGAGCAGCA AGTAGAGGAC 
CTGGAGTGCT CCCTGGAGCA AGAGAAGAAG CTTCGCATGG ATACAGAACG TGCCAAGCGC 
AAGCTCGAAG GAGACCTGAA GCTGACGCAG GAGACAGTGA CAGACACCAC ACAAGACAAG 
CAGCAGCTGG AGGAGAAGTT GAAGAAGAAG GATTCAGAGC TGAGTCAGCT GAGCCTGCGG 
GTGGAGGATG AGCAGCTCCT GGGGGCGCAG TTGCAGAAGA AGATCAAGGA GCTACAGGCG 
AGGGCTGAGG AGCTGGAGGA GGAGCTGGAG GCGGAGCGAG CAGCCCGTGC CCGTGTGGAA 
AAGCAGCGAG CTGAGGCAGC CCGAGAGCTG GAGGAGCTGA GTGAGAGGCT AGAGGAGGCT 
GGTGGAGCCT CTGCCGGGCA GCGTGAGGGT TGCCGCAAGC GTGAGGCTGA GTTGGGCCGA 
CTGCGGCGGG AGCTGGAGGA GACAGTTCTG AGACACGAGG CCACTGTGGC TGCCCTGCGC 
CGCAAGCAGG CTGATAGCGC CGCAGAGTTG AGCGAGCAGG TAGACAGCCT CCAGCGGATC 
CGGCAGAAAC TGGAGAAGGA AAAGAGTGAG CTGCGCATGG AGGTGGATGA TCTGGGGGCC 
AGCGTGGAAA CTCTGGCCCG TGGCAAGGCC AGTGCAGAGA AGCTGTGCCG GACCTATGAG 
GACCAGCTCA GCGAGGCCAA GATCAAAGTG GAGGAGCTGC AGCGGCAGCT GACTGACGCC 
AGCACCCAGC GGGGGCGGTT ACAAACGGAG AACGGGGAGC TGGGCCGCCT GCTTGAGGAG 
AAGGAGTCTA TGATCAGCCA GCTGAGCCGC GGGAAGACCT CAGCCGCTCA GAGTCTGGAG 
GAACTTCGGC GGCAGTTGGA GGAAGAAAGC AAGGCCAAGG GTGCGCTGGC TCACGCAGTG 
CAGGCTCTGC GGCACGACTG CGACCTCCTG CGAGAGCAGC ATGAGGAAGA GTCCGAGGCG 
CAGGCTGAGC TGCAGCGGCT GCTGTCCAAA GCCAACGCTG AGGTGGCACA GTGGAGGAGC 
AAGTATGAGG CGGACGCCAT CCAAAGGACG GAGGAGCTGG AGGAGGCCAA GAAGAAGCTG 
GCCCTGAGGC TGCAGGAGGC AGAAGAAGGC GTAGAGGCTG CGAATGCCAA GTGCTCCTCA 
CTGGAGAAGG CCAAGCTGCG GCTGCAGACA GAGTCCGAGG ACGTGACCTT GGAGCTGGAG 
CGGGCCACCT CAGCCGCTGC TGCACTGGAT AAGAAGCAGC GGCACTTGGA GCGGGCCCTG 
GAGGAACGGA GGCGGCAGGA GGAGGAGATG CAGCGGGAAC TGGAGGCGGC GCAGAGGGAG 
GCTCGAGGTC TGGGCACCGA GCTCTTTCGA TTGCGGCACA GCCATGAGGA GGCGCTTGAG 
GCTCTGGAGA CACTCAAGCG GGAGAACAAG AATCTGCAGG AGGAGATCAG CGACCTTACA 
GACCAGGTCA GCCTCAGCGG GAAGAGCATC CAGGAACTGG AGAAGGCCAA GAAGGCGCTG 
GAAGGGGAGA AGAGTGAGCT GCAGGCTGCG CTGGAGGAGG CTGAGGGGGC GCTGGAGCTG 
GAAGAGACAA AGACCCTGCG GATCCAGTTG GAGTTGTCCC AGGTCAAGGC GGAAGTGGAC 
CGAAAGCTGG CGGAGAAGGA TGAGGAGTGT ACTAACCTGA GGCGCAACCA CCAGCGGGCT 
GTGGAGTCCT TGCAGGCCTC CCTGGATGCC GAGACTAGGG CCCGCAATGA AGCACTGCGT 
CTCAAGAAGA AGATGGAGGG GGACCTCAAT GACCTGGAGT TACAGCTGGG CCACGCAACC 
CGCCAGGCCA TGGAGGCACA GGCAGCCACG AGGCTGCTTC AGGCCCAACT CAAGGAGGAG 
CAAGCAGGGC GTGATGAGGA GCAGAGGCTG GCTGCCGAGC TCCGCGAGCA GGGCCAGGCC 
CTCGAGCGCA GGGCTGCACT GCTGGCATCT GAGCTGGAAG AGCTTCGGGC CGCCCTGGAA 
CAGGGCGAGC GCAGCCGAAG GCTGGCTGAA CAGGAGCTGT TGGAGGCCAC AGAGCGCCTC 
AACCTACTGC ATTCACAGAA CACAGGCCTC CTGAACCAGA AAAAGAAGCT GGAGGTGGAC 
TTGGCCCAGC TGAGTGGGGA GGTGGAGGAG GCTGCGCAGG AGAGGCGGGA AGCTGAGGAG 
AAGGCCAAAA AGGCCATCAC GGATGCAGCC ATGATGGCTG AGGAGCTGAA AAAGGAGCAG 
GACACAAGTG CACACCTCGA ACGGATGAAG AAGACCCTGG AACAGACAGT GCGGGAGCTG 
CAGGCCCGCC TTGAGGAAGC AGAGCAGGCC GCACTCCGGG GTGGGAAGAA GCAAGTGCAG 
AAGCTGGAAG CCAAGGTGCG GGAACTGGAG GCAGAGCTTG ACGCAGAGCA GAAGAAGCAC 
GCTGAGGCCC TCAAGGGTGT GCGCAAACAT GAGCGCAGGG TCAAGGAGCT TGCGTACCAG 
ACTGAGGAAG ACAGGAAGAA CCTGGCTCGC ATGCAGGACC TAGTGGACAA GCTGCAGAGC 
AAGGTCAAGA GCTACAAGCG TCAGTTTGAG GAGGCGGAGC AGCAGGCCAG TACCAACCTG 
GCTAAGTACC GCAAGGCCCA GCACGAGTTA GACGACGCTG AGGAGCGGGC AGACATGGCA 
GAGACCCAGG CCAACAAGCT GCGGGCAAGG TCCAGGGATG CCCTGGGTCC CAAGGTAAGG 
TGGCCACTGT GTTGGGTGGA CACTGGGGAG ACACGCACGG GTGACCTAGG TCAGCCTCTT 
TCTCATCTCT TAGCACAAGG AGTGAGAGAC TCCAGCTCTG ACGAGGGATA CCCCGTGCTC 
CTGTTGTCTT TCCACCTGCC ACTCCACAGA GCTCTGGAAC CCCAAATAAA CACCACATCT 
GCAGCAGCAG CCGCTGTCCT GTCCTTATTT CTTGCGGCTA GAGGAGCTGG GAACAGAACC 
TCATGGACAA AGTCAGGCCC ACGTCAATCT TTAAAATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

l-Arginine Attenuates Cardiac Dysfunction, But Further Down-Regulates ?-Myosin Heavy Chain Expression in Isoproterenol-Induced Cardiomyopathy.
Basic & clinical pharmacology & toxicology117(4)251-60(2015 Oct)
Kralova E,Doka G,Pivackova L,Srankova J,Kuracinova K,Janega P,Babal P,Klimas J,Krenek P

Developmental expression and cardiac transcriptional regulation of Myh7b, a third myosin heavy chain in the vertebrate heart.
Cytoskeleton (Hoboken, N.J.)69(5)324-35(2012 May)
Warkman AS,Whitman SA,Miller MK,Garriock RJ,Schwach CM,Gregorio CC,Krieg PA

Regulation of synapse structure and function by distinct myosin II motors.
The Journal of neuroscience : the official journal of the Society for Neuroscience31(4)1448-60(2011 Jan)
Rubio MD,Johnson R,Miller CA,Huganir RL,Rumbaugh G

Two novel/ancient myosins in mammalian skeletal muscles: MYH14/7b and MYH15 are expressed in extraocular muscles and muscle spindles.
The Journal of physiology588(Pt 2)353-64(2010 Jan)
Rossi AC,Mammucari C,Argentini C,Reggiani C,Schiaffino S

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Myh7b cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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