Acss2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Acss2
Entrez Gene ID	311569
Full Name	acyl-CoA synthetase short-chain family member 2
Synonyms	Acas2
General protein information	Preferred Names acyl-CoA synthetase short-chain family member 2 Names acetyl-coenzyme A synthetase, cytoplasmic acetyl-Coenzyme A synthetase 2 (ADP forming)
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 3 Map Location: 3q42

mRNA and Protein(s)

mRNA	Protein	Name
XM_006235321.3	XP_006235383.2	acetyl-coenzyme A synthetase, cytoplasmic isoform X1
NM_001107793.1	NP_001101263.1	acetyl-coenzyme A synthetase, cytoplasmic

Pathways from BioSystems

Homologies

	ACSS2	XP_001103094.1
Canis lupus familiaris (dog)	ACSS2	XP_534395.4
Mus musculus (house mouse)	Acss2	NP_062785.2
Rattus norvegicus (Norway rat)	Acss2	NP_001101263.1
Caenorhabditis elegans (roundworm)	acs-19	NP_001021205.1
Arabidopsis thaliana (thale cress)	ACS	NP_001031974.2
Pan troglodytes (chimpanzee)	ACSS2	XP_514806.3
Oryza sativa (rice)	Os02g0525900	NP_001046995.1
Bos taurus (cattle)	ACSS2	NP_001098809.1
Drosophila melanogaster (fruit fly)	AcCoAS	NP_001014599.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP006569	XP_316594.2
Xenopus tropicalis (tropical clawed frog)	acss2	XP_004918555.1
Homo sapiens (human)	ACSS2	NP_001070020.2
Gallus gallus (chicken)	ACSS2	XP_417342.4

Gene Ontology
Bibliography

Related articles in PubMed

ACSS2 gene variant associated with cleft lip and palate in two independent Hispanic populations.
Dodhia S, Celis K, Aylward A, Cai Y, Fontana ME, Trespalacios A, Hoffman DC, Alfonso HO, Eisig SB, Su GH, Chung WK, Haddad J Jr
The Laryngoscope127(10)E336-E339(2017 Oct)

Molecular cloning of rat acss3 and characterization of mammalian propionyl-CoA synthetase in the liver mitochondrial matrix.
Yoshimura Y, Araki A, Maruta H, Takahashi Y, Yamashita H
Journal of biochemistry161(3)279-289(2017 Mar)

Glucose-independent Acetate Metabolism Promotes Melanoma Cell Survival and Tumor Growth.
Lakhter AJ, Hamilton J, Konger RL, Brustovetsky N, Broxmeyer HE, Naidu SR
The Journal of biological chemistry291(42)21869-21879(2016 Oct)

Using Whole Exome Sequencing to Identify Candidate Genes With Rare Variants In Nonsyndromic Cleft Lip and Palate.
Aylward A, Cai Y, Lee A, Blue E, Rabinowitz D, Haddad J Jr,
Genetic epidemiology40(5)432-41(2016 07)

Ketone body production is differentially altered in steatosis and non-alcoholic steatohepatitis in obese humans.
M?nnist? VT, Simonen M, Hyysalo J, Soininen P, Kangas AJ, Kaminska D, Matte AK, Venesmaa S, K?kel? P, K?rj? V, Arola J, Gylling H, Cederberg H, Kuusisto J, Laakso M, Yki-J?rvinen H, Ala-Korpela M, Pihlajam?ki J
Liver international : official journal of the International Association for the Study of the Liver35(7)1853-61(2015 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Acss2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Acss2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa00185	NM_001107793.1 Latest version!	Rattus norvegicus acyl-CoA synthetase short-chain family member 2 (Acss2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$412.30 $589.00
ORa60416	XM_006235321.3 Latest version!	Rattus norvegicus acyl-CoA synthetase short-chain family member 2 (Acss2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$391.30 $559.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Acss2 ( NM_001107793.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001101263.1 Rattus norvegicus acyl-CoA synthetase short-chain family member 2 (Acss2), mRNA.

Price & Availability↑

CloneID	ORa00185
Clone ID Related Accession (Same CDS sequence)	NM_001107793.1
Accession Version	NM_001107793.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2106bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-09
Organism	Rattus norvegicus(Norway rat)
Product	acetyl-coenzyme A synthetase, cytoplasmic
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CH474050.2. On or before Oct 4, 2007 this sequence version replaced XM_230773.4, XM_001064684.1. ##Evidence-Data-START## Transcript exon combination :: BC161963.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGGGCTTC CCGAGGAGCG GCGCAAGAGC GGCAGCGGGA GCCGGGCTCG CGAGGAGACC 
GGCGCCGAGG GCCGGGTGCG GGGTTGGTCC CCGCCGCCGG AGGTCAGACG CTCGGCGCAC 
GTCCCCTCTC TGCAGCGCTA CCGCGAGCTG CACCGGCGTT CTGTGGAGGA GCCACGGGAG 
TTTTGGGGAA ACATTGCCAA GGAATTTTAC TGGAAAACCC CATGCCCTGG CCCATTCCTC 
CAGTACAACT TTGATGTGAC TAAAGGGAAA ATATTCACTG AGTGGATGAA AGGAGCAACC 
ACAAACATCT GCTACAATGT GCTGGATAGA AATGTCCATG AGAAAAAACT TGGCGACAAA 
GTTGCTTTTT ACTGGGAGGG CAATGAGCCA GGGGAGACCA CCAAGATCAC ATACCGTGAA 
CTCCTGGTCC AGGTGTGTCA GTTCAGCAAT GTTCTCCGTA AACAGGGTGT TCAGAAGGGT 
GACCGAGTGG CCATCTACAT GCCTATGATC TTGGAGCTTG TGGTGGCTAT GCTGGCATGT 
GCTCGCCTTG GAGCTTTGCA CTCCATTGTG TTTGCAGGCT TCTCTGCAGA GTCTCTCTGT 
GAACGGATCT TGGATTCCAG CTGCTGCCTG CTCATTACTA CAGATGCCTT CTACAGGGGG 
GAAAAACTTG TGAACCTGAA GGAGCTGGCT GATGAGTCCT TGGAGAAGTG CCGAGAGAAG 
GGTTTCCCAG TGAGATGCTG CATTGTGGTC AAACATCTGG GGCGGGCAGA GCTGGGCATG 
AATGACTCCC CCAGCCAGTC CCCACCCGTT AAGAGGCCAT GTCCAGATGT CCAGATCTCC 
TGGAATGAAG GGGTTGACTT GTGGTGGCAT GAACTCATGC AGCAGGCAGG AGATGAGTTT 
GAGCCTGAGT GGTGTGATGC CGAGGACCCT CTCTTCATCT TGTATACCAG TGGCTCCACA 
GGCAAACCCA AGGGTGTGGT GCACACAGTT GGAGGCTACA TGCTCTATGT GGCTACAACT 
TTCAAGTATG TGTTTGATTT CCATCCGGAG GATGTATTCT GGTGCACAGC AGACATTGGC 
TGGATCACTG GTCATTCCTA TGTCACCTAT GGGCCGCTGG CGAATGGTGC CACCAGTGTT 
TTGTTTGAGG GGATCCCCAC ATACCCAGAT GAAGGCCGCT TGTGGAGCAT TGTGGACAAA 
TACAAGGTGA CCAAGTTCTA CACGGCACCA ACAGCAATCC GGATGCTCAT GAAGTTTGGA 
GATGATCCTG TCACCAAGCA TAGCCGGGCA TCCTTGCAGG TGCTGGGCAC AGTAGGTGAA 
CCCATCAACC CTGAAGCCTG GCTATGGTAC CATCGGGTAG TAGGTTCCCA GCGTTGCCCC 
ATTGTAGACA CCTTCTGGCA AACAGAAACA GGTGGCCATA TGCTGACCCC TCTCCCTGGT 
GCCACACCCA TGAAACCAGG TTCTGCTTCT TTCCCGTTCT TTGGTGTTGC GCCTGCAATC 
CTGAACGAGT CGGGGGAAGA ACTGGAAGGG GAAGCTGAAG GTTATCTGGT GTTCAAGCAG 
CCCTGGCCAG GGATCATGCG CACAGTTTAT GGGAACCACA CACGTTTCGA GACCACCTAC 
TTTAAGAAGT TCCCTGGCTA CTACGTGACT GGAGATGGCT GCCGACGGGA TCAGGACGGC 
TATTACTGGA TCACTGGCAG GATTGATGAC ATGCTCAATG TGTCTGGACA TCTTCTGAGT 
ACAGCAGAGG TGGAATCAGC ACTTGTGGAA CATGAGGCTG TTGCAGAGGC AGCTGTGGTG 
GGCCACCCTC ATCCTGTGAA GGGTGAATGC CTCTACTGCT TTGTTACCTT GTGTGACGGC 
CATACCTTCA GCCCCACCCT CACTGAGGAA CTCAAGAAGC AGATTAGAGA AAAGATTGGC 
CCCATTGCCA CACCAGACTA CATCCAGAAT GCACCCGGCT TGCCTAAAAC ACGCTCAGGA 
AAAATCATGA GGAGAGTACT CCGGAAGATT GCTCAGAATG ACCACGACCT GGGGGATACC 
TCTACAGTGG CTGACCCATC TGTCATCAAC CATCTCTTCA GTCACCGCTG CCTGACCACA 
CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001101263.1
CDS	65..2170
Translation	MGLPEERRKSGSGSRAREETGAEGRVRGWSPPPEVRRSAHVPSLQRYRELHRRSVEEPREFWGNIAKEFYWKTPCPGPFLQYNFDVTKGKIFTEWMKGATTNICYNVLDRNVHEKKLGDKVAFYWEGNEPGETTKITYRELLVQVCQFSNVLRKQGVQKGDRVAIYMPMILELVVAMLACARLGALHSIVFAGFSAESLCERILDSSCCLLITTDAFYRGEKLVNLKELADESLEKCREKGFPVRCCIVVKHLGRAELGMNDSPSQSPPVKRPCPDVQISWNEGVDLWWHELMQQAGDEFEPEWCDAEDPLFILYTSGSTGKPKGVVHTVGGYMLYVATTFKYVFDFHPEDVFWCTADIGWITGHSYVTYGPLANGATSVLFEGIPTYPDEGRLWSIVDKYKVTKFYTAPTAIRMLMKFGDDPVTKHSRASLQVLGTVGEPINPEAWLWYHRVVGSQRCPIVDTFWQTETGGHMLTPLPGATPMKPGSASFPFFGVAPAILNESGEELEGEAEGYLVFKQPWPGIMRTVYGNHTRFETTYFKKFPGYYVTGDGCRRDQDGYYWITGRIDDMLNVSGHLLSTAEVESALVEHEAVAEAAVVGHPHPVKGECLYCFVTLCDGHTFSPTLTEELKKQIREKIGPIATPDYIQNAPGLPKTRSGKIMRRVLRKIAQNDHDLGDTSTVADPSVINHLFSHRCLTTQ

Target ORF information:

RefSeq Version	NM_001107793.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus acyl-CoA synthetase short-chain family member 2 (Acss2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001107793.1

ORF Insert Sequence:

ATGGGGCTTC CCGAGGAGCG GCGCAAGAGC GGCAGCGGGA GCCGGGCTCG CGAGGAGACC 
GGCGCCGAGG GCCGGGTGCG GGGTTGGTCC CCGCCGCCGG AGGTCAGACG CTCGGCGCAC 
GTCCCCTCTC TGCAGCGCTA CCGCGAGCTG CACCGGCGTT CTGTGGAGGA GCCACGGGAG 
TTTTGGGGAA ACATTGCCAA GGAATTTTAC TGGAAAACCC CATGCCCTGG CCCATTCCTC 
CAGTACAACT TTGATGTGAC TAAAGGGAAA ATATTCACTG AGTGGATGAA AGGAGCAACC 
ACAAACATCT GCTACAATGT GCTGGATAGA AATGTCCATG AGAAAAAACT TGGCGACAAA 
GTTGCTTTTT ACTGGGAGGG CAATGAGCCA GGGGAGACCA CCAAGATCAC ATACCGTGAA 
CTCCTGGTCC AGGTGTGTCA GTTCAGCAAT GTTCTCCGTA AACAGGGTGT TCAGAAGGGT 
GACCGAGTGG CCATCTACAT GCCTATGATC TTGGAGCTTG TGGTGGCTAT GCTGGCATGT 
GCTCGCCTTG GAGCTTTGCA CTCCATTGTG TTTGCAGGCT TCTCTGCAGA GTCTCTCTGT 
GAACGGATCT TGGATTCCAG CTGCTGCCTG CTCATTACTA CAGATGCCTT CTACAGGGGG 
GAAAAACTTG TGAACCTGAA GGAGCTGGCT GATGAGTCCT TGGAGAAGTG CCGAGAGAAG 
GGTTTCCCAG TGAGATGCTG CATTGTGGTC AAACATCTGG GGCGGGCAGA GCTGGGCATG 
AATGACTCCC CCAGCCAGTC CCCACCCGTT AAGAGGCCAT GTCCAGATGT CCAGATCTCC 
TGGAATGAAG GGGTTGACTT GTGGTGGCAT GAACTCATGC AGCAGGCAGG AGATGAGTTT 
GAGCCTGAGT GGTGTGATGC CGAGGACCCT CTCTTCATCT TGTATACCAG TGGCTCCACA 
GGCAAACCCA AGGGTGTGGT GCACACAGTT GGAGGCTACA TGCTCTATGT GGCTACAACT 
TTCAAGTATG TGTTTGATTT CCATCCGGAG GATGTATTCT GGTGCACAGC AGACATTGGC 
TGGATCACTG GTCATTCCTA TGTCACCTAT GGGCCGCTGG CGAATGGTGC CACCAGTGTT 
TTGTTTGAGG GGATCCCCAC ATACCCAGAT GAAGGCCGCT TGTGGAGCAT TGTGGACAAA 
TACAAGGTGA CCAAGTTCTA CACGGCACCA ACAGCAATCC GGATGCTCAT GAAGTTTGGA 
GATGATCCTG TCACCAAGCA TAGCCGGGCA TCCTTGCAGG TGCTGGGCAC AGTAGGTGAA 
CCCATCAACC CTGAAGCCTG GCTATGGTAC CATCGGGTAG TAGGTTCCCA GCGTTGCCCC 
ATTGTAGACA CCTTCTGGCA AACAGAAACA GGTGGCCATA TGCTGACCCC TCTCCCTGGT 
GCCACACCCA TGAAACCAGG TTCTGCTTCT TTCCCGTTCT TTGGTGTTGC GCCTGCAATC 
CTGAACGAGT CGGGGGAAGA ACTGGAAGGG GAAGCTGAAG GTTATCTGGT GTTCAAGCAG 
CCCTGGCCAG GGATCATGCG CACAGTTTAT GGGAACCACA CACGTTTCGA GACCACCTAC 
TTTAAGAAGT TCCCTGGCTA CTACGTGACT GGAGATGGCT GCCGACGGGA TCAGGACGGC 
TATTACTGGA TCACTGGCAG GATTGATGAC ATGCTCAATG TGTCTGGACA TCTTCTGAGT 
ACAGCAGAGG TGGAATCAGC ACTTGTGGAA CATGAGGCTG TTGCAGAGGC AGCTGTGGTG 
GGCCACCCTC ATCCTGTGAA GGGTGAATGC CTCTACTGCT TTGTTACCTT GTGTGACGGC 
CATACCTTCA GCCCCACCCT CACTGAGGAA CTCAAGAAGC AGATTAGAGA AAAGATTGGC 
CCCATTGCCA CACCAGACTA CATCCAGAAT GCACCCGGCT TGCCTAAAAC ACGCTCAGGA 
AAAATCATGA GGAGAGTACT CCGGAAGATT GCTCAGAATG ACCACGACCT GGGGGATACC 
TCTACAGTGG CTGACCCATC TGTCATCAAC CATCTCTTCA GTCACCGCTG CCTGACCACA 
CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Acss2 ( XM_006235321.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006235383.2 Rattus norvegicus acyl-CoA synthetase short-chain family member 2 (Acss2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa60416
Clone ID Related Accession (Same CDS sequence)	XM_006235321.3
Accession Version	XM_006235321.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2145bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	acetyl-coenzyme A synthetase, cytoplasmic isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005102.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006235321.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGCTTC CCGAGGAGCG GCGCAAGAGC GGCAGCGGGA GCCGGGCTCG CGAGGAGACC 
GGCGCCGAGG GCCGGGTGCG GGGTTGGTCC CCGCCGCCGG AGGTCAGACG CTCGGCGCAC 
GTCCCCTCTC TGCAGCGCTA CCGCGAGCTG CACCGGCGTT CTGTGGAGGA GCCACGGGAG 
TTTTGGGGAA ACATTGCCAA GGAATTTTAC TGGAAAACCC CATGCCCTGG CCCATTCCTC 
CAGTACAACT TTGATGTGAC TAAAGGGAAA ATATTCACTG AGTGGATGAA AGGAGCAACC 
ACAAACATCT GCTACAATGT GCTGGATAGA AATGTCCATG AGAAAAAACT TGGCGACAAA 
GTTGCTTTTT ACTGGGAGGG CAATGAGCCA GGGGAGACCA CCAAGATCAC ATACCGTGAA 
CTCCTGGTCC AGGTGTGTCA GTTCAGCAAT GTTCTCCGTA AACAGGGTGT TCAGAAGGGT 
GACCGAGTGG CCATCTACAT GCCTATGATC TTGGAGCTTG TGGTGGCTAT GCTGGCATGT 
GCTCGCCTTG GAGCTTTGCA CTCCATTGTG TTTGCAGGCT TCTCTGCAGA GTCTCTCTGT 
GAACGGATCT TGGATTCCAG CTGCTGCCTG CTCATTACTA CAGATGCCTT CTACAGGGGG 
GAAAAACTTG TGAACCTGAA GGAGCTGGCT GATGAGTCCT TGGAGAAGTG CCGAGAGAAG 
GGTTTCCCAG TGAGATGCTG CATTGTGGTC AAACATCTGG GGCGGGCAGA GCTGGGCATG 
AATGACTCCC CCAGCCAGTC CCCACCCGTT AAGAGGCCAT GTCCAGATGT CCAGGGTAAG 
CTGAAAGAGA AATCCAAGTG CATCCGACCC CAGATCTCCT GGAATGAAGG GGTTGACTTG 
TGGTGGCATG AACTCATGCA GCAGGCAGGA GATGAGTTTG AGCCTGAGTG GTGTGATGCC 
GAGGACCCTC TCTTCATCTT GTATACCAGT GGCTCCACAG GCAAACCCAA GGGTGTGGTG 
CACACAGTTG GAGGCTACAT GCTCTATGTG GCTACAACTT TCAAGTATGT GTTTGATTTC 
CATCCGGAGG ATGTATTCTG GTGCACAGCA GACATTGGCT GGATCACTGG TCATTCCTAT 
GTCACCTATG GGCCGCTGGC GAATGGTGCC ACCAGTGTTT TGTTTGAGGG GATCCCCACA 
TACCCAGATG AAGGCCGCTT GTGGAGCATT GTGGACAAAT ACAAGGTGAC CAAGTTCTAC 
ACGGCACCAA CAGCAATCCG GATGCTCATG AAGTTTGGAG ATGATCCTGT CACCAAGCAT 
AGCCGGGCAT CCTTGCAGGT GCTGGGCACA GTAGGTGAAC CCATCAACCC TGAAGCCTGG 
CTATGGTACC ATCGGGTAGT AGGTTCCCAG CGTTGCCCCA TTGTAGACAC CTTCTGGCAA 
ACAGAAACAG GTGGCCATAT GCTGACCCCT CTCCCTGGTG CCACACCCAT GAAACCAGGT 
TCTGCTTCTT TCCCGTTCTT TGGTGTTGCG CCTGCAATCC TGAACGAGTC GGGGGAAGAA 
CTGGAAGGGG AAGCTGAAGG TTATCTGGTG TTCAAGCAGC CCTGGCCAGG GATCATGCGC 
ACAGTTTATG GGAACCACAC ACGTTTCGAG ACCACCTACT TTAAGAAGTT CCCTGGCTAC 
TACGTGACTG GAGATGGCTG CCGACGGGAT CAGGACGGCT ATTACTGGAT CACTGGCAGG 
ATTGATGACA TGCTCAATGT GTCTGGACAT CTTCTGAGTA CAGCAGAGGT GGAATCAGCA 
CTTGTGGAAC ATGAGGCTGT TGCAGAGGCA GCTGTGGTGG GCCACCCTCA TCCTGTGAAG 
GGTGAATGCC TCTACTGCTT TGTTACCTTG TGTGACGGCC ATACCTTCAG CCCCACCCTC 
ACTGAGGAAC TCAAGAAGCA GATTAGAGAA AAGATTGGCC CCATTGCCAC ACCAGACTAC 
ATCCAGAATG CACCCGGCTT GCCTAAAACA CGCTCAGGAA AAATCATGAG GAGAGTACTC 
CGGAAGATTG CTCAGAATGA CCACGACCTG GGGGATACCT CTACAGTGGC TGACCCATCT 
GTCATCAACC ATCTCTTCAG TCACCGCTGC CTGACCACAC AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006235383.2
CDS	92..2236
Translation	MGLPEERRKSGSGSRAREETGAEGRVRGWSPPPEVRRSAHVPSLQRYRELHRRSVEEPREFWGNIAKEFYWKTPCPGPFLQYNFDVTKGKIFTEWMKGATTNICYNVLDRNVHEKKLGDKVAFYWEGNEPGETTKITYRELLVQVCQFSNVLRKQGVQKGDRVAIYMPMILELVVAMLACARLGALHSIVFAGFSAESLCERILDSSCCLLITTDAFYRGEKLVNLKELADESLEKCREKGFPVRCCIVVKHLGRAELGMNDSPSQSPPVKRPCPDVQGKLKEKSKCIRPQISWNEGVDLWWHELMQQAGDEFEPEWCDAEDPLFILYTSGSTGKPKGVVHTVGGYMLYVATTFKYVFDFHPEDVFWCTADIGWITGHSYVTYGPLANGATSVLFEGIPTYPDEGRLWSIVDKYKVTKFYTAPTAIRMLMKFGDDPVTKHSRASLQVLGTVGEPINPEAWLWYHRVVGSQRCPIVDTFWQTETGGHMLTPLPGATPMKPGSASFPFFGVAPAILNESGEELEGEAEGYLVFKQPWPGIMRTVYGNHTRFETTYFKKFPGYYVTGDGCRRDQDGYYWITGRIDDMLNVSGHLLSTAEVESALVEHEAVAEAAVVGHPHPVKGECLYCFVTLCDGHTFSPTLTEELKKQIREKIGPIATPDYIQNAPGLPKTRSGKIMRRVLRKIAQNDHDLGDTSTVADPSVINHLFSHRCLTTQ

Target ORF information:

RefSeq Version	XM_006235321.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus acyl-CoA synthetase short-chain family member 2 (Acss2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006235321.3

ORF Insert Sequence:

ATGGGGCTTC CCGAGGAGCG GCGCAAGAGC GGCAGCGGGA GCCGGGCTCG CGAGGAGACC 
GGCGCCGAGG GCCGGGTGCG GGGTTGGTCC CCGCCGCCGG AGGTCAGACG CTCGGCGCAC 
GTCCCCTCTC TGCAGCGCTA CCGCGAGCTG CACCGGCGTT CTGTGGAGGA GCCACGGGAG 
TTTTGGGGAA ACATTGCCAA GGAATTTTAC TGGAAAACCC CATGCCCTGG CCCATTCCTC 
CAGTACAACT TTGATGTGAC TAAAGGGAAA ATATTCACTG AGTGGATGAA AGGAGCAACC 
ACAAACATCT GCTACAATGT GCTGGATAGA AATGTCCATG AGAAAAAACT TGGCGACAAA 
GTTGCTTTTT ACTGGGAGGG CAATGAGCCA GGGGAGACCA CCAAGATCAC ATACCGTGAA 
CTCCTGGTCC AGGTGTGTCA GTTCAGCAAT GTTCTCCGTA AACAGGGTGT TCAGAAGGGT 
GACCGAGTGG CCATCTACAT GCCTATGATC TTGGAGCTTG TGGTGGCTAT GCTGGCATGT 
GCTCGCCTTG GAGCTTTGCA CTCCATTGTG TTTGCAGGCT TCTCTGCAGA GTCTCTCTGT 
GAACGGATCT TGGATTCCAG CTGCTGCCTG CTCATTACTA CAGATGCCTT CTACAGGGGG 
GAAAAACTTG TGAACCTGAA GGAGCTGGCT GATGAGTCCT TGGAGAAGTG CCGAGAGAAG 
GGTTTCCCAG TGAGATGCTG CATTGTGGTC AAACATCTGG GGCGGGCAGA GCTGGGCATG 
AATGACTCCC CCAGCCAGTC CCCACCCGTT AAGAGGCCAT GTCCAGATGT CCAGGGTAAG 
CTGAAAGAGA AATCCAAGTG CATCCGACCC CAGATCTCCT GGAATGAAGG GGTTGACTTG 
TGGTGGCATG AACTCATGCA GCAGGCAGGA GATGAGTTTG AGCCTGAGTG GTGTGATGCC 
GAGGACCCTC TCTTCATCTT GTATACCAGT GGCTCCACAG GCAAACCCAA GGGTGTGGTG 
CACACAGTTG GAGGCTACAT GCTCTATGTG GCTACAACTT TCAAGTATGT GTTTGATTTC 
CATCCGGAGG ATGTATTCTG GTGCACAGCA GACATTGGCT GGATCACTGG TCATTCCTAT 
GTCACCTATG GGCCGCTGGC GAATGGTGCC ACCAGTGTTT TGTTTGAGGG GATCCCCACA 
TACCCAGATG AAGGCCGCTT GTGGAGCATT GTGGACAAAT ACAAGGTGAC CAAGTTCTAC 
ACGGCACCAA CAGCAATCCG GATGCTCATG AAGTTTGGAG ATGATCCTGT CACCAAGCAT 
AGCCGGGCAT CCTTGCAGGT GCTGGGCACA GTAGGTGAAC CCATCAACCC TGAAGCCTGG 
CTATGGTACC ATCGGGTAGT AGGTTCCCAG CGTTGCCCCA TTGTAGACAC CTTCTGGCAA 
ACAGAAACAG GTGGCCATAT GCTGACCCCT CTCCCTGGTG CCACACCCAT GAAACCAGGT 
TCTGCTTCTT TCCCGTTCTT TGGTGTTGCG CCTGCAATCC TGAACGAGTC GGGGGAAGAA 
CTGGAAGGGG AAGCTGAAGG TTATCTGGTG TTCAAGCAGC CCTGGCCAGG GATCATGCGC 
ACAGTTTATG GGAACCACAC ACGTTTCGAG ACCACCTACT TTAAGAAGTT CCCTGGCTAC 
TACGTGACTG GAGATGGCTG CCGACGGGAT CAGGACGGCT ATTACTGGAT CACTGGCAGG 
ATTGATGACA TGCTCAATGT GTCTGGACAT CTTCTGAGTA CAGCAGAGGT GGAATCAGCA 
CTTGTGGAAC ATGAGGCTGT TGCAGAGGCA GCTGTGGTGG GCCACCCTCA TCCTGTGAAG 
GGTGAATGCC TCTACTGCTT TGTTACCTTG TGTGACGGCC ATACCTTCAG CCCCACCCTC 
ACTGAGGAAC TCAAGAAGCA GATTAGAGAA AAGATTGGCC CCATTGCCAC ACCAGACTAC 
ATCCAGAATG CACCCGGCTT GCCTAAAACA CGCTCAGGAA AAATCATGAG GAGAGTACTC 
CGGAAGATTG CTCAGAATGA CCACGACCTG GGGGATACCT CTACAGTGGC TGACCCATCT 
GTCATCAACC ATCTCTTCAG TCACCGCTGC CTGACCACAC AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

ACSS2 gene variant associated with cleft lip and palate in two independent Hispanic populations.
The Laryngoscope127(10)E336-E339(2017 Oct)
Dodhia S,Celis K,Aylward A,Cai Y,Fontana ME,Trespalacios A,Hoffman DC,Alfonso HO,Eisig SB,Su GH,Chung WK,Haddad J Jr

Molecular cloning of rat acss3 and characterization of mammalian propionyl-CoA synthetase in the liver mitochondrial matrix.
Journal of biochemistry161(3)279-289(2017 Mar)
Yoshimura Y,Araki A,Maruta H,Takahashi Y,Yamashita H

Glucose-independent Acetate Metabolism Promotes Melanoma Cell Survival and Tumor Growth.
The Journal of biological chemistry291(42)21869-21879(2016 Oct)
Lakhter AJ,Hamilton J,Konger RL,Brustovetsky N,Broxmeyer HE,Naidu SR

Using Whole Exome Sequencing to Identify Candidate Genes With Rare Variants In Nonsyndromic Cleft Lip and Palate.
Genetic epidemiology40(5)432-41(2016 07)
Aylward A,Cai Y,Lee A,Blue E,Rabinowitz D,Haddad J Jr,

Ketone body production is differentially altered in steatosis and non-alcoholic steatohepatitis in obese humans.
Liver international : official journal of the International Association for the Study of the Liver35(7)1853-61(2015 Jul)
M?nnist? VT,Simonen M,Hyysalo J,Soininen P,Kangas AJ,Kaminska D,Matte AK,Venesmaa S,K?kel? P,K?rj? V,Arola J,Gylling H,Cederberg H,Kuusisto J,Laakso M,Yki-J?rvinen H,Ala-Korpela M,Pihlajam?ki J

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Acss2 cDNA ORF clone, Rattus norvegicus(Norway rat)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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