Itch cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Itch
Entrez Gene ID	311567
Full Name	itchy E3 ubiquitin protein ligase
General protein information	Preferred Names itchy E3 ubiquitin protein ligase Names E3 ubiquitin-protein ligase Itchy homolog itch E3 ubiquitin ligase itchy E3 ubiquitin protein ligase homolog
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 3 Map Location: 3q41

mRNA and Protein(s)

mRNA	Protein	Name
XM_008762335.2	XP_008760557.1	E3 ubiquitin-protein ligase Itchy homolog isoform X1
XM_008762336.2	XP_008760558.1	E3 ubiquitin-protein ligase Itchy homolog isoform X1
NM_001005887.1	NP_001005887.1	E3 ubiquitin-protein ligase Itchy homolog

Pathways from BioSystems

Homologies

Homo sapiens (human)	ITCH	NP_113671.3
Canis lupus familiaris (dog)	ITCH	XP_851460.1
Mus musculus (house mouse)	Itch	NP_001230641.1
Rattus norvegicus (Norway rat)	Itch	NP_001005887.1
Bos taurus (cattle)	ITCH	NP_001075897.1
Gallus gallus (chicken)	ITCH	XP_417330.2
Danio rerio (zebrafish)	itch	XP_002667578.2
Danio rerio (zebrafish)	LOC100330031	XP_003201702.2
	LOC707640	XP_002808005.1
Xenopus tropicalis (tropical clawed frog)	itch	NP_001072737.1

Gene Ontology
Bibliography

Related articles in PubMed

HECT-Type Ubiquitin E3 Ligase ITCH Interacts With Thioredoxin-Interacting Protein and Ameliorates Reactive Oxygen Species-Induced Cardiotoxicity.
Otaki Y, Takahashi H, Watanabe T, Funayama A, Netsu S, Honda Y, Narumi T, Kadowaki S, Hasegawa H, Honda S, Arimoto T, Shishido T, Miyamoto T, Kamata H, Nakajima O, Kubota I
Journal of the American Heart Association5(1)(2016 Jan)

Nedd4 family interacting protein 1 (Ndfip1) is required for ubiquitination and nuclear trafficking of BRCA1-associated ATM activator 1 (BRAT1) during the DNA damage response.
Low LH, Chow YL, Li Y, Goh CP, Putz U, Silke J, Ouchi T, Howitt J, Tan SS
The Journal of biological chemistry290(11)7141-50(2015 Mar)

The ubiquitin ligase deltex-3l regulates endosomal sorting of the G protein-coupled receptor CXCR4.
Holleman J, Marchese A
Molecular biology of the cell25(12)1892-904(2014 Jun)

?-arrestin 1 (ARRDC1) and ?-arrestins cooperate to mediate Notch degradation in mammals.
Puca L, Chastagner P, Meas-Yedid V, Isra?l A, Brou C
Journal of cell science126(Pt 19)4457-68(2013 Oct)

Regulation of NF-?B by ubiquitination.
Chen J, Chen ZJ
Current opinion in immunology25(1)4-12(2013 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

ITCH targets TXNIP for ubiquitin-proteasome degradation in cardiomyocytes and ameliorates reactive oxygen species-induced cardiotoxicity through the thioredoxin system.
Title: HECT-Type Ubiquitin E3 Ligase ITCH Interacts With Thioredoxin-Interacting Protein and Ameliorates Reactive Oxygen Species-Induced Cardiotoxicity.

The following Itch gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Itch cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa10792	NM_001005887.1 Latest version!	Rattus norvegicus itchy E3 ubiquitin protein ligase (Itch), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
ORa47039	XM_008762335.2 Latest version!	Rattus norvegicus itchy E3 ubiquitin protein ligase (Itch), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
ORa47039	XM_008762336.2 Latest version!	Rattus norvegicus itchy E3 ubiquitin protein ligase (Itch), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Itch ( NM_001005887.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001005887.1 Rattus norvegicus itchy E3 ubiquitin protein ligase (Itch), mRNA.

Price & Availability↑

CloneID	ORa10792
Clone ID Related Accession (Same CDS sequence)	NM_001005887.1
Accession Version	NM_001005887.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2565bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-30
Organism	Rattus norvegicus(Norway rat)
Product	E3 ubiquitin-protein ligase Itchy homolog
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AY600518.1. On Oct 22, 2004 this sequence version replaced XM_230770.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY600518.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGGTAGTC TGACCATGAA ATCTCAACTT CAGATTACTG TCATCTCAGC CAAACTTAAA 
GAAAATAAAA AGAATTGGTT TGGACCAAGT CCTTATGTAG AAGTCACAGT AGATGGGCAG 
TCAAAGAAGA CAGAAAAATG CAACAATACA AACAGCCCCA AGTGGAAGCA GCCCCTCACA 
GTTATCGTTA CCCCTATGAG TAAATTATGT TTTCGTGTGT GGAGTCACCA GACCCTGAAA 
TCTGATGTTT TATTGGGAAC TGCTGGATTA GATATTTATG AAACATTAAA GTCAAACAAT 
ATGAAACTTG AGGAAGTAGT TATGACTTTG CAGCTTGTAG GTGACAAAGA GCCAACAGAG 
ACAATGGGAG ATTTGTCAGT TTGTCTTGAT GGGCTGCAAG TAGAAGCTGA AGTTGTTACT 
AATGGTGAAA CTTCATGCTC AGAGGGTGCT ACACAGAATG ACGCTGGCTG CAGACCCAAA 
GATGAAACAA GAGTGAGCAC AAATGGATCA GAGGACCCTG AGGTTGCAGC CTCGGGGGAG 
AACAAGAGGG CCAATGGGAA CAATTCTCCA TCCCTTTCAA ATGGTGGTTT TAAGCCTTCT 
AGACCTCCTA GACCTTCAAG ACCACCTCCA CCCACCCCAC GAAGACCAGC TTCTGTCAAT 
GGCTCACCAT CCACGAACTC TGACAGCGAT GGATCTAGCA CAGGCTCTTT GCCACCCACA 
AATACAAATG TAAATTCAAG TGCATCGGAA GGAGCAGCAT CTGGGTTAAT AATTCCTCGT 
ACCATCTCTG GGGGCTCAGG CCCTAGGCCG CTGAATACTG TCAGTCAAGC TCCCCTCCCA 
CCTGGGTGGG AGCAGAGAGT AGATCAGCAT GGGCGTGCTT ACTATGTAGA CCATGTTGAA 
AAGCGAACAA CACGGGATAG ACCGGAACCT CTACCTCCTG GCTGGGAACG GCGTGTGGAC 
AATATGGGAC GTATTTATTA TGTTGACCAT TTCACGAGAA CCACAACATG GCAGAGGCCG 
ACCTTGGAAT CTGTCCGGAA CTATGAGCAG TGGCAGCTCC AGCGTAGTCA ACTTCAGGGA 
GCAATGCAGC AGTTTAACCA GAGATTCATC TTTGGGAATC AAGATTTGTT TGCTACATCA 
CAAAACAAAG AGTTTGATCC CCTTGGTCCT TTGCCCCCTG GATGGGAGAA GAGAACTGAT 
AGCAACGGCA GAGTGTATTT TGTCAACCAC AACACTCGGA TTACACAGTG GGAAGACCCC 
AGAAGCCAAG GTCAGTTAAA TGAAAAGCCC TTACCAGAAG GCTGGGAAAT GCGATTCACA 
GTGGATGGAA TTCCATATTT TGTGGACCAC AATAGAAGAG CAACTACCTA CATAGATCCA 
CGCACAGGAA AGTCAGCCTT AGACAACGGA CCCCAGATAG CCTATGTTCG GGACTTCAAG 
GCAAAAGTTC AGTATTTCCG GTTCTGGTGT CAGCAACTGG CCATGCCACA GCATATAAAG 
ATCACAGTGA CAAGAAAAAC ATTGTTTGAG GATTCCTTTC AACAGATCAT GAGCTTCAGT 
CCGCAAGATC TGAGAAGACG TTTGTGGGTG ATTTTCCCAG GAGAAGAAGG TTTAGATTAT 
GGAGGTGTAG CAAGAGAATG GTTCTTTCTT TTGTCACATG AAGTGTTGAA CCCAATGTAT 
TGCCTGTTTG AATATGCAGG GAAGGATAAC TACTGCCTGC AGATAAACCC CGCTTCTTAT 
ATCAACCCAG ACCACCTGAA ATACTTCCGT TTTATTGGCA GATTTATTGC CATGGCTCTA 
TTCCATGGGA AATTCATAGA TACCGGATTT TCTTTACCAT TCTATAAACG TATCTTGAAT 
AAACCAGTGG GACTTAAGGA TTTAGAATCT ATCGATCCAG AATTTTACAA TTCTCTCATC 
TGGGTTAAAG AAAACAATAT CGAGGAATGT GGTTTGGAAA TGTACTTCTC AGTTGATAAA 
GAAATTCTGG GTGAAATTAA GAGTCATGAT TTGAAACCCA ATGGTGGCAA TATTCTGGTG 
ACAGAAGAAA ATAAGGAAGA GTACATCAGG ATGGTCGCTG AGTGGAGGTT GTCTCGGGGA 
GTTGAAGAGC AGACACAAGC TTTCTTTGAG GGCTTTAATG AAATTCTTCC TCAGCAATAT 
TTGCAATATT TTGATGCGAA GGAATTAGAG GTCCTTTTGT GTGGGATGCA GGAGATCGAT 
TTGAATGACT GGCAGAGACA CGCCATCTAC CGTCACTATA CCAGGACGAG CAAGCAGATC 
GTGTGGTTTT GGCAGTTTGT TAAAGAAATT GATAACGAGA AGAGGATGCG ACTTCTGCAG 
TTTGTTACCG GAACCTGCCG ATTGCCAGTG GGAGGATTTG CTGACCTCAT GGGGAGTAAT 
GGACCACAGA AGTTCTGCAT CGAAAAAGTT GGCAAAGAAA ATTGGTTACC CAGAAGCCAT 
ACTTGTTTTA ACCGCCTGGA CCTCCCACCC TACAAGAGCT ATGAGCAACT GAAGGAAAAG 
CTGTTATTTG CCATTGAAGA AACTGAAGGA TTTGGACAAG AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001005887.1
CDS	1..2565
Translation	MGSLTMKSQLQITVISAKLKENKKNWFGPSPYVEVTVDGQSKKTEKCNNTNSPKWKQPLTVIVTPMSKLCFRVWSHQTLKSDVLLGTAGLDIYETLKSNNMKLEEVVMTLQLVGDKEPTETMGDLSVCLDGLQVEAEVVTNGETSCSEGATQNDAGCRPKDETRVSTNGSEDPEVAASGENKRANGNNSPSLSNGGFKPSRPPRPSRPPPPTPRRPASVNGSPSTNSDSDGSSTGSLPPTNTNVNSSASEGAASGLIIPRTISGGSGPRPLNTVSQAPLPPGWEQRVDQHGRAYYVDHVEKRTTRDRPEPLPPGWERRVDNMGRIYYVDHFTRTTTWQRPTLESVRNYEQWQLQRSQLQGAMQQFNQRFIFGNQDLFATSQNKEFDPLGPLPPGWEKRTDSNGRVYFVNHNTRITQWEDPRSQGQLNEKPLPEGWEMRFTVDGIPYFVDHNRRATTYIDPRTGKSALDNGPQIAYVRDFKAKVQYFRFWCQQLAMPQHIKITVTRKTLFEDSFQQIMSFSPQDLRRRLWVIFPGEEGLDYGGVAREWFFLLSHEVLNPMYCLFEYAGKDNYCLQINPASYINPDHLKYFRFIGRFIAMALFHGKFIDTGFSLPFYKRILNKPVGLKDLESIDPEFYNSLIWVKENNIEECGLEMYFSVDKEILGEIKSHDLKPNGGNILVTEENKEEYIRMVAEWRLSRGVEEQTQAFFEGFNEILPQQYLQYFDAKELEVLLCGMQEIDLNDWQRHAIYRHYTRTSKQIVWFWQFVKEIDNEKRMRLLQFVTGTCRLPVGGFADLMGSNGPQKFCIEKVGKENWLPRSHTCFNRLDLPPYKSYEQLKEKLLFAIEETEGFGQE

Target ORF information:

RefSeq Version	NM_001005887.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus itchy E3 ubiquitin protein ligase (Itch), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001005887.1

ORF Insert Sequence:

ATGGGTAGTC TGACCATGAA ATCTCAACTT CAGATTACTG TCATCTCAGC CAAACTTAAA 
GAAAATAAAA AGAATTGGTT TGGACCAAGT CCTTATGTAG AAGTCACAGT AGATGGGCAG 
TCAAAGAAGA CAGAAAAATG CAACAATACA AACAGCCCCA AGTGGAAGCA GCCCCTCACA 
GTTATCGTTA CCCCTATGAG TAAATTATGT TTTCGTGTGT GGAGTCACCA GACCCTGAAA 
TCTGATGTTT TATTGGGAAC TGCTGGATTA GATATTTATG AAACATTAAA GTCAAACAAT 
ATGAAACTTG AGGAAGTAGT TATGACTTTG CAGCTTGTAG GTGACAAAGA GCCAACAGAG 
ACAATGGGAG ATTTGTCAGT TTGTCTTGAT GGGCTGCAAG TAGAAGCTGA AGTTGTTACT 
AATGGTGAAA CTTCATGCTC AGAGGGTGCT ACACAGAATG ACGCTGGCTG CAGACCCAAA 
GATGAAACAA GAGTGAGCAC AAATGGATCA GAGGACCCTG AGGTTGCAGC CTCGGGGGAG 
AACAAGAGGG CCAATGGGAA CAATTCTCCA TCCCTTTCAA ATGGTGGTTT TAAGCCTTCT 
AGACCTCCTA GACCTTCAAG ACCACCTCCA CCCACCCCAC GAAGACCAGC TTCTGTCAAT 
GGCTCACCAT CCACGAACTC TGACAGCGAT GGATCTAGCA CAGGCTCTTT GCCACCCACA 
AATACAAATG TAAATTCAAG TGCATCGGAA GGAGCAGCAT CTGGGTTAAT AATTCCTCGT 
ACCATCTCTG GGGGCTCAGG CCCTAGGCCG CTGAATACTG TCAGTCAAGC TCCCCTCCCA 
CCTGGGTGGG AGCAGAGAGT AGATCAGCAT GGGCGTGCTT ACTATGTAGA CCATGTTGAA 
AAGCGAACAA CACGGGATAG ACCGGAACCT CTACCTCCTG GCTGGGAACG GCGTGTGGAC 
AATATGGGAC GTATTTATTA TGTTGACCAT TTCACGAGAA CCACAACATG GCAGAGGCCG 
ACCTTGGAAT CTGTCCGGAA CTATGAGCAG TGGCAGCTCC AGCGTAGTCA ACTTCAGGGA 
GCAATGCAGC AGTTTAACCA GAGATTCATC TTTGGGAATC AAGATTTGTT TGCTACATCA 
CAAAACAAAG AGTTTGATCC CCTTGGTCCT TTGCCCCCTG GATGGGAGAA GAGAACTGAT 
AGCAACGGCA GAGTGTATTT TGTCAACCAC AACACTCGGA TTACACAGTG GGAAGACCCC 
AGAAGCCAAG GTCAGTTAAA TGAAAAGCCC TTACCAGAAG GCTGGGAAAT GCGATTCACA 
GTGGATGGAA TTCCATATTT TGTGGACCAC AATAGAAGAG CAACTACCTA CATAGATCCA 
CGCACAGGAA AGTCAGCCTT AGACAACGGA CCCCAGATAG CCTATGTTCG GGACTTCAAG 
GCAAAAGTTC AGTATTTCCG GTTCTGGTGT CAGCAACTGG CCATGCCACA GCATATAAAG 
ATCACAGTGA CAAGAAAAAC ATTGTTTGAG GATTCCTTTC AACAGATCAT GAGCTTCAGT 
CCGCAAGATC TGAGAAGACG TTTGTGGGTG ATTTTCCCAG GAGAAGAAGG TTTAGATTAT 
GGAGGTGTAG CAAGAGAATG GTTCTTTCTT TTGTCACATG AAGTGTTGAA CCCAATGTAT 
TGCCTGTTTG AATATGCAGG GAAGGATAAC TACTGCCTGC AGATAAACCC CGCTTCTTAT 
ATCAACCCAG ACCACCTGAA ATACTTCCGT TTTATTGGCA GATTTATTGC CATGGCTCTA 
TTCCATGGGA AATTCATAGA TACCGGATTT TCTTTACCAT TCTATAAACG TATCTTGAAT 
AAACCAGTGG GACTTAAGGA TTTAGAATCT ATCGATCCAG AATTTTACAA TTCTCTCATC 
TGGGTTAAAG AAAACAATAT CGAGGAATGT GGTTTGGAAA TGTACTTCTC AGTTGATAAA 
GAAATTCTGG GTGAAATTAA GAGTCATGAT TTGAAACCCA ATGGTGGCAA TATTCTGGTG 
ACAGAAGAAA ATAAGGAAGA GTACATCAGG ATGGTCGCTG AGTGGAGGTT GTCTCGGGGA 
GTTGAAGAGC AGACACAAGC TTTCTTTGAG GGCTTTAATG AAATTCTTCC TCAGCAATAT 
TTGCAATATT TTGATGCGAA GGAATTAGAG GTCCTTTTGT GTGGGATGCA GGAGATCGAT 
TTGAATGACT GGCAGAGACA CGCCATCTAC CGTCACTATA CCAGGACGAG CAAGCAGATC 
GTGTGGTTTT GGCAGTTTGT TAAAGAAATT GATAACGAGA AGAGGATGCG ACTTCTGCAG 
TTTGTTACCG GAACCTGCCG ATTGCCAGTG GGAGGATTTG CTGACCTCAT GGGGAGTAAT 
GGACCACAGA AGTTCTGCAT CGAAAAAGTT GGCAAAGAAA ATTGGTTACC CAGAAGCCAT 
ACTTGTTTTA ACCGCCTGGA CCTCCCACCC TACAAGAGCT ATGAGCAACT GAAGGAAAAG 
CTGTTATTTG CCATTGAAGA AACTGAAGGA TTTGGACAAG AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Itch ( XM_008762335.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008760557.1 Rattus norvegicus itchy E3 ubiquitin protein ligase (Itch), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa47039
Clone ID Related Accession (Same CDS sequence)	XM_008762335.2 , XM_008762336.2
Accession Version	XM_008762335.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2595bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	E3 ubiquitin-protein ligase Itchy homolog isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005102.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008762335.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGACA GTGCACCACA GCTCGATTCA ATGGGTAGCC TGACCATGAA ATCTCAACTT 
CAGATTACTG TCATCTCAGC CAAACTTAAA GAAAATAAAA AGAATTGGTT TGGACCAAGT 
CCTTATGTAG AAGTCACAGT AGATGGGCAG TCAAAGAAGA CAGAAAAATG CAACAATACA 
AACAGCCCCA AGTGGAAGCA GCCCCTCACA GTTATCGTTA CCCCTATGAG TAAATTATGT 
TTTCGTGTGT GGAGTCACCA GACCCTGAAA TCTGATGTTT TATTGGGAAC TGCTGGATTA 
GATATTTATG AAACATTAAA GTCAAACAAT ATGAAACTTG AGGAAGTAGT TATGACTTTG 
CAGCTTGTAG GTGACAAAGA GCCAACAGAG ACAATGGGAG ATTTGTCAGT TTGTCTTGAT 
GGGCTGCAAG TAGAAGCTGA AGTTGTTACT AATGGTGAAA CTTCATGCTC AGAGGGTGCT 
ACACAGAATG ACGCTGGCTG CAGACCCAAA GATGAAACAA GAGTGAGCAC AAATGGATCA 
GAGGACCCTG AGGTTGCAGC CTCGGGGGAA AACAAGAGGG CCAATGGGAA CAATTCTCCA 
TCCCTTTCAA ATGGTGGTTT TAAGCCTTCT AGACCTCCTA GACCTTCAAG ACCACCTCCA 
CCCACCCCAC GAAGACCAGC TTCTGTCAAT GGCTCACCAT CCACGAACTC TGACAGCGAT 
GGATCTAGCA CAGGCTCTTT GCCACCCACA AATACAAATG TAAATTCAAG TGCATCGGAA 
GGAGCAGCAT CTGGGTTAAT AATTCCTCTG ACCATCTCTG GGGGCTCAGG CCCTAGGCCG 
CTGAATACTG TCAGTCAAGC TCCCCTCCCA CCTGGGTGGG AGCAGAGAGT AGATCAGCAT 
GGGCGTGTTT ACTATGTAGA CCATGTTGAA AAGCGAACAA CATGGGATAG ACCGGAACCT 
CTACCTCCTG GCTGGGAACG GCGTGTGGAC AATATGGGAC GTATTTATTA TGTTGACCAT 
TTCACGAGAA CCACAACATG GCAGAGGCCG ACCTTGGAAT CTGTCCGGAA CTATGAGCAG 
TGGCAGCTCC AGCGTAGTCA ACTTCAGGGA GCAATGCAGC AGTTTAACCA GAGATTCATC 
TTTGGGAATC AAGATTTGTT TGCTACATCA CAAAACAAAG AGTTTGATCC CCTTGGTCCT 
TTGCCCCCTG GATGGGAGAA GAGAACTGAT AGCAACGGCA GAGTGTATTT TGTCAACCAC 
AACACTCGGA TTACACAGTG GGAAGACCCC AGAAGCCAAG GTCAGTTAAA TGAAAAGCCC 
TTACCAGAAG GCTGGGAAAT GCGATTCACA GTGGATGGAA TTCCATATTT TGTGGACCAC 
AATAGAAGAG CAACTACCTA CATAGATCCA CGCACAGGAA AGTCAGCCTT AGACAACGGA 
CCCCAGATAG CCTATGTTCG GGACTTCAAG GCAAAAGTTC AGTATTTCCG GTTCTGGTGT 
CAGCAACTGG CCATGCCACA GCATATAAAG ATCACAGTGA CAAGAAAAAC ATTGTTTGAG 
GATTCCTTTC AACAGATCAT GAGCTTCAGT CCGCAAGATC TGAGAAGACG TTTGTGGGTG 
ATTTTCCCAG GAGAAGAAGG TTTAGATTAT GGAGGTGTAG CAAGAGAATG GTTCTTTCTT 
TTGTCACATG AAGTGTTGAA CCCAATGTAT TGCCTGTTTG AATATGCAGG GAAGGATAAC 
TACTGCCTGC AGATAAACCC CGCTTCTTAT ATCAACCCAG ACCACCTGAA ATACTTCCGT 
TTTATTGGCA GATTTATTGC CATGGCTCTA TTCCATGGGA AATTCATAGA TACCGGATTT 
TCTTTACCAT TCTATAAACG TATCTTGAAT AAACCAGTGG GACTTAAGGA TTTAGAATCT 
ATCGATCCAG AATTTTACAA TTCTCTCATC TGGGTTAAAG AAAACAATAT CGAGGAATGT 
GGTTTGGAAA TGTACTTCTC AGTTGATAAA GAAATTCTGG GTGAAATTAA GAGTCATGAT 
TTGAAACCCA ATGGTGGCAA TATTCTGGTG ACAGAAGAAA ATAAGGAAGA GTACATCAGG 
ATGGTCGCTG AGTGGAGGTT GTCTCGGGGA GTTGAAGAGC AGACACAAGC TTTCTTTGAG 
GGCTTTAATG AAATTCTTCC TCAGCAATAT TTGCAATATT TTGATGCGAA GGAATTAGAG 
GTCCTTTTGT GTGGGATGCA GGAGATCGAT TTGAATGACT GGCAGAGACA CGCCATCTAC 
CGTCACTATA CCAGGACGAG CAAGCAGATC GTGTGGTTTT GGCAGTTTGT TAAAGAAATT 
GATAACGAGA AGAGGATGCG ACTTCTGCAG TTTGTTACCG GAACCTGCCG ATTGCCAGTG 
GGAGGATTTG CTGACCTCAT GGGGAGTAAT GGACCACAGA AGTTCTGCAT CGAAAAAGTT 
GGCAAAGAAA ATTGGTTACC CAGAAGCCAT ACTTGTTTTA ACCGCCTGGA CCTCCCACCC 
TACAAGAGCT ATGAGCAACT GAAGGAAAAG CTGTTATTTG CCATTGAAGA AACAGAAGGA 
TTTGGACAAG AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008760557.1
CDS	203..2797
Translation	MSDSAPQLDSMGSLTMKSQLQITVISAKLKENKKNWFGPSPYVEVTVDGQSKKTEKCNNTNSPKWKQPLTVIVTPMSKLCFRVWSHQTLKSDVLLGTAGLDIYETLKSNNMKLEEVVMTLQLVGDKEPTETMGDLSVCLDGLQVEAEVVTNGETSCSEGATQNDAGCRPKDETRVSTNGSEDPEVAASGENKRANGNNSPSLSNGGFKPSRPPRPSRPPPPTPRRPASVNGSPSTNSDSDGSSTGSLPPTNTNVNSSASEGAASGLIIPLTISGGSGPRPLNTVSQAPLPPGWEQRVDQHGRVYYVDHVEKRTTWDRPEPLPPGWERRVDNMGRIYYVDHFTRTTTWQRPTLESVRNYEQWQLQRSQLQGAMQQFNQRFIFGNQDLFATSQNKEFDPLGPLPPGWEKRTDSNGRVYFVNHNTRITQWEDPRSQGQLNEKPLPEGWEMRFTVDGIPYFVDHNRRATTYIDPRTGKSALDNGPQIAYVRDFKAKVQYFRFWCQQLAMPQHIKITVTRKTLFEDSFQQIMSFSPQDLRRRLWVIFPGEEGLDYGGVAREWFFLLSHEVLNPMYCLFEYAGKDNYCLQINPASYINPDHLKYFRFIGRFIAMALFHGKFIDTGFSLPFYKRILNKPVGLKDLESIDPEFYNSLIWVKENNIEECGLEMYFSVDKEILGEIKSHDLKPNGGNILVTEENKEEYIRMVAEWRLSRGVEEQTQAFFEGFNEILPQQYLQYFDAKELEVLLCGMQEIDLNDWQRHAIYRHYTRTSKQIVWFWQFVKEIDNEKRMRLLQFVTGTCRLPVGGFADLMGSNGPQKFCIEKVGKENWLPRSHTCFNRLDLPPYKSYEQLKEKLLFAIEETEGFGQE

Target ORF information:

RefSeq Version	XM_008762335.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus itchy E3 ubiquitin protein ligase (Itch), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008762335.2

ORF Insert Sequence:

ATGTCTGACA GTGCACCACA GCTCGATTCA ATGGGTAGCC TGACCATGAA ATCTCAACTT 
CAGATTACTG TCATCTCAGC CAAACTTAAA GAAAATAAAA AGAATTGGTT TGGACCAAGT 
CCTTATGTAG AAGTCACAGT AGATGGGCAG TCAAAGAAGA CAGAAAAATG CAACAATACA 
AACAGCCCCA AGTGGAAGCA GCCCCTCACA GTTATCGTTA CCCCTATGAG TAAATTATGT 
TTTCGTGTGT GGAGTCACCA GACCCTGAAA TCTGATGTTT TATTGGGAAC TGCTGGATTA 
GATATTTATG AAACATTAAA GTCAAACAAT ATGAAACTTG AGGAAGTAGT TATGACTTTG 
CAGCTTGTAG GTGACAAAGA GCCAACAGAG ACAATGGGAG ATTTGTCAGT TTGTCTTGAT 
GGGCTGCAAG TAGAAGCTGA AGTTGTTACT AATGGTGAAA CTTCATGCTC AGAGGGTGCT 
ACACAGAATG ACGCTGGCTG CAGACCCAAA GATGAAACAA GAGTGAGCAC AAATGGATCA 
GAGGACCCTG AGGTTGCAGC CTCGGGGGAA AACAAGAGGG CCAATGGGAA CAATTCTCCA 
TCCCTTTCAA ATGGTGGTTT TAAGCCTTCT AGACCTCCTA GACCTTCAAG ACCACCTCCA 
CCCACCCCAC GAAGACCAGC TTCTGTCAAT GGCTCACCAT CCACGAACTC TGACAGCGAT 
GGATCTAGCA CAGGCTCTTT GCCACCCACA AATACAAATG TAAATTCAAG TGCATCGGAA 
GGAGCAGCAT CTGGGTTAAT AATTCCTCTG ACCATCTCTG GGGGCTCAGG CCCTAGGCCG 
CTGAATACTG TCAGTCAAGC TCCCCTCCCA CCTGGGTGGG AGCAGAGAGT AGATCAGCAT 
GGGCGTGTTT ACTATGTAGA CCATGTTGAA AAGCGAACAA CATGGGATAG ACCGGAACCT 
CTACCTCCTG GCTGGGAACG GCGTGTGGAC AATATGGGAC GTATTTATTA TGTTGACCAT 
TTCACGAGAA CCACAACATG GCAGAGGCCG ACCTTGGAAT CTGTCCGGAA CTATGAGCAG 
TGGCAGCTCC AGCGTAGTCA ACTTCAGGGA GCAATGCAGC AGTTTAACCA GAGATTCATC 
TTTGGGAATC AAGATTTGTT TGCTACATCA CAAAACAAAG AGTTTGATCC CCTTGGTCCT 
TTGCCCCCTG GATGGGAGAA GAGAACTGAT AGCAACGGCA GAGTGTATTT TGTCAACCAC 
AACACTCGGA TTACACAGTG GGAAGACCCC AGAAGCCAAG GTCAGTTAAA TGAAAAGCCC 
TTACCAGAAG GCTGGGAAAT GCGATTCACA GTGGATGGAA TTCCATATTT TGTGGACCAC 
AATAGAAGAG CAACTACCTA CATAGATCCA CGCACAGGAA AGTCAGCCTT AGACAACGGA 
CCCCAGATAG CCTATGTTCG GGACTTCAAG GCAAAAGTTC AGTATTTCCG GTTCTGGTGT 
CAGCAACTGG CCATGCCACA GCATATAAAG ATCACAGTGA CAAGAAAAAC ATTGTTTGAG 
GATTCCTTTC AACAGATCAT GAGCTTCAGT CCGCAAGATC TGAGAAGACG TTTGTGGGTG 
ATTTTCCCAG GAGAAGAAGG TTTAGATTAT GGAGGTGTAG CAAGAGAATG GTTCTTTCTT 
TTGTCACATG AAGTGTTGAA CCCAATGTAT TGCCTGTTTG AATATGCAGG GAAGGATAAC 
TACTGCCTGC AGATAAACCC CGCTTCTTAT ATCAACCCAG ACCACCTGAA ATACTTCCGT 
TTTATTGGCA GATTTATTGC CATGGCTCTA TTCCATGGGA AATTCATAGA TACCGGATTT 
TCTTTACCAT TCTATAAACG TATCTTGAAT AAACCAGTGG GACTTAAGGA TTTAGAATCT 
ATCGATCCAG AATTTTACAA TTCTCTCATC TGGGTTAAAG AAAACAATAT CGAGGAATGT 
GGTTTGGAAA TGTACTTCTC AGTTGATAAA GAAATTCTGG GTGAAATTAA GAGTCATGAT 
TTGAAACCCA ATGGTGGCAA TATTCTGGTG ACAGAAGAAA ATAAGGAAGA GTACATCAGG 
ATGGTCGCTG AGTGGAGGTT GTCTCGGGGA GTTGAAGAGC AGACACAAGC TTTCTTTGAG 
GGCTTTAATG AAATTCTTCC TCAGCAATAT TTGCAATATT TTGATGCGAA GGAATTAGAG 
GTCCTTTTGT GTGGGATGCA GGAGATCGAT TTGAATGACT GGCAGAGACA CGCCATCTAC 
CGTCACTATA CCAGGACGAG CAAGCAGATC GTGTGGTTTT GGCAGTTTGT TAAAGAAATT 
GATAACGAGA AGAGGATGCG ACTTCTGCAG TTTGTTACCG GAACCTGCCG ATTGCCAGTG 
GGAGGATTTG CTGACCTCAT GGGGAGTAAT GGACCACAGA AGTTCTGCAT CGAAAAAGTT 
GGCAAAGAAA ATTGGTTACC CAGAAGCCAT ACTTGTTTTA ACCGCCTGGA CCTCCCACCC 
TACAAGAGCT ATGAGCAACT GAAGGAAAAG CTGTTATTTG CCATTGAAGA AACAGAAGGA 
TTTGGACAAG AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Itch ( XM_008762336.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008760558.1 Rattus norvegicus itchy E3 ubiquitin protein ligase (Itch), transcript variant X2, mRNA.

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CloneID	ORa47039
Clone ID Related Accession (Same CDS sequence)	XM_008762335.2 , XM_008762336.2
Accession Version	XM_008762336.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2595bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	E3 ubiquitin-protein ligase Itchy homolog isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005102.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008762336.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTGACA GTGCACCACA GCTCGATTCA ATGGGTAGCC TGACCATGAA ATCTCAACTT 
CAGATTACTG TCATCTCAGC CAAACTTAAA GAAAATAAAA AGAATTGGTT TGGACCAAGT 
CCTTATGTAG AAGTCACAGT AGATGGGCAG TCAAAGAAGA CAGAAAAATG CAACAATACA 
AACAGCCCCA AGTGGAAGCA GCCCCTCACA GTTATCGTTA CCCCTATGAG TAAATTATGT 
TTTCGTGTGT GGAGTCACCA GACCCTGAAA TCTGATGTTT TATTGGGAAC TGCTGGATTA 
GATATTTATG AAACATTAAA GTCAAACAAT ATGAAACTTG AGGAAGTAGT TATGACTTTG 
CAGCTTGTAG GTGACAAAGA GCCAACAGAG ACAATGGGAG ATTTGTCAGT TTGTCTTGAT 
GGGCTGCAAG TAGAAGCTGA AGTTGTTACT AATGGTGAAA CTTCATGCTC AGAGGGTGCT 
ACACAGAATG ACGCTGGCTG CAGACCCAAA GATGAAACAA GAGTGAGCAC AAATGGATCA 
GAGGACCCTG AGGTTGCAGC CTCGGGGGAA AACAAGAGGG CCAATGGGAA CAATTCTCCA 
TCCCTTTCAA ATGGTGGTTT TAAGCCTTCT AGACCTCCTA GACCTTCAAG ACCACCTCCA 
CCCACCCCAC GAAGACCAGC TTCTGTCAAT GGCTCACCAT CCACGAACTC TGACAGCGAT 
GGATCTAGCA CAGGCTCTTT GCCACCCACA AATACAAATG TAAATTCAAG TGCATCGGAA 
GGAGCAGCAT CTGGGTTAAT AATTCCTCTG ACCATCTCTG GGGGCTCAGG CCCTAGGCCG 
CTGAATACTG TCAGTCAAGC TCCCCTCCCA CCTGGGTGGG AGCAGAGAGT AGATCAGCAT 
GGGCGTGTTT ACTATGTAGA CCATGTTGAA AAGCGAACAA CATGGGATAG ACCGGAACCT 
CTACCTCCTG GCTGGGAACG GCGTGTGGAC AATATGGGAC GTATTTATTA TGTTGACCAT 
TTCACGAGAA CCACAACATG GCAGAGGCCG ACCTTGGAAT CTGTCCGGAA CTATGAGCAG 
TGGCAGCTCC AGCGTAGTCA ACTTCAGGGA GCAATGCAGC AGTTTAACCA GAGATTCATC 
TTTGGGAATC AAGATTTGTT TGCTACATCA CAAAACAAAG AGTTTGATCC CCTTGGTCCT 
TTGCCCCCTG GATGGGAGAA GAGAACTGAT AGCAACGGCA GAGTGTATTT TGTCAACCAC 
AACACTCGGA TTACACAGTG GGAAGACCCC AGAAGCCAAG GTCAGTTAAA TGAAAAGCCC 
TTACCAGAAG GCTGGGAAAT GCGATTCACA GTGGATGGAA TTCCATATTT TGTGGACCAC 
AATAGAAGAG CAACTACCTA CATAGATCCA CGCACAGGAA AGTCAGCCTT AGACAACGGA 
CCCCAGATAG CCTATGTTCG GGACTTCAAG GCAAAAGTTC AGTATTTCCG GTTCTGGTGT 
CAGCAACTGG CCATGCCACA GCATATAAAG ATCACAGTGA CAAGAAAAAC ATTGTTTGAG 
GATTCCTTTC AACAGATCAT GAGCTTCAGT CCGCAAGATC TGAGAAGACG TTTGTGGGTG 
ATTTTCCCAG GAGAAGAAGG TTTAGATTAT GGAGGTGTAG CAAGAGAATG GTTCTTTCTT 
TTGTCACATG AAGTGTTGAA CCCAATGTAT TGCCTGTTTG AATATGCAGG GAAGGATAAC 
TACTGCCTGC AGATAAACCC CGCTTCTTAT ATCAACCCAG ACCACCTGAA ATACTTCCGT 
TTTATTGGCA GATTTATTGC CATGGCTCTA TTCCATGGGA AATTCATAGA TACCGGATTT 
TCTTTACCAT TCTATAAACG TATCTTGAAT AAACCAGTGG GACTTAAGGA TTTAGAATCT 
ATCGATCCAG AATTTTACAA TTCTCTCATC TGGGTTAAAG AAAACAATAT CGAGGAATGT 
GGTTTGGAAA TGTACTTCTC AGTTGATAAA GAAATTCTGG GTGAAATTAA GAGTCATGAT 
TTGAAACCCA ATGGTGGCAA TATTCTGGTG ACAGAAGAAA ATAAGGAAGA GTACATCAGG 
ATGGTCGCTG AGTGGAGGTT GTCTCGGGGA GTTGAAGAGC AGACACAAGC TTTCTTTGAG 
GGCTTTAATG AAATTCTTCC TCAGCAATAT TTGCAATATT TTGATGCGAA GGAATTAGAG 
GTCCTTTTGT GTGGGATGCA GGAGATCGAT TTGAATGACT GGCAGAGACA CGCCATCTAC 
CGTCACTATA CCAGGACGAG CAAGCAGATC GTGTGGTTTT GGCAGTTTGT TAAAGAAATT 
GATAACGAGA AGAGGATGCG ACTTCTGCAG TTTGTTACCG GAACCTGCCG ATTGCCAGTG 
GGAGGATTTG CTGACCTCAT GGGGAGTAAT GGACCACAGA AGTTCTGCAT CGAAAAAGTT 
GGCAAAGAAA ATTGGTTACC CAGAAGCCAT ACTTGTTTTA ACCGCCTGGA CCTCCCACCC 
TACAAGAGCT ATGAGCAACT GAAGGAAAAG CTGTTATTTG CCATTGAAGA AACAGAAGGA 
TTTGGACAAG AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008760558.1
CDS	183..2777
Translation	MSDSAPQLDSMGSLTMKSQLQITVISAKLKENKKNWFGPSPYVEVTVDGQSKKTEKCNNTNSPKWKQPLTVIVTPMSKLCFRVWSHQTLKSDVLLGTAGLDIYETLKSNNMKLEEVVMTLQLVGDKEPTETMGDLSVCLDGLQVEAEVVTNGETSCSEGATQNDAGCRPKDETRVSTNGSEDPEVAASGENKRANGNNSPSLSNGGFKPSRPPRPSRPPPPTPRRPASVNGSPSTNSDSDGSSTGSLPPTNTNVNSSASEGAASGLIIPLTISGGSGPRPLNTVSQAPLPPGWEQRVDQHGRVYYVDHVEKRTTWDRPEPLPPGWERRVDNMGRIYYVDHFTRTTTWQRPTLESVRNYEQWQLQRSQLQGAMQQFNQRFIFGNQDLFATSQNKEFDPLGPLPPGWEKRTDSNGRVYFVNHNTRITQWEDPRSQGQLNEKPLPEGWEMRFTVDGIPYFVDHNRRATTYIDPRTGKSALDNGPQIAYVRDFKAKVQYFRFWCQQLAMPQHIKITVTRKTLFEDSFQQIMSFSPQDLRRRLWVIFPGEEGLDYGGVAREWFFLLSHEVLNPMYCLFEYAGKDNYCLQINPASYINPDHLKYFRFIGRFIAMALFHGKFIDTGFSLPFYKRILNKPVGLKDLESIDPEFYNSLIWVKENNIEECGLEMYFSVDKEILGEIKSHDLKPNGGNILVTEENKEEYIRMVAEWRLSRGVEEQTQAFFEGFNEILPQQYLQYFDAKELEVLLCGMQEIDLNDWQRHAIYRHYTRTSKQIVWFWQFVKEIDNEKRMRLLQFVTGTCRLPVGGFADLMGSNGPQKFCIEKVGKENWLPRSHTCFNRLDLPPYKSYEQLKEKLLFAIEETEGFGQE

Target ORF information:

RefSeq Version	XM_008762336.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus itchy E3 ubiquitin protein ligase (Itch), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008762336.2

ORF Insert Sequence:

ATGTCTGACA GTGCACCACA GCTCGATTCA ATGGGTAGCC TGACCATGAA ATCTCAACTT 
CAGATTACTG TCATCTCAGC CAAACTTAAA GAAAATAAAA AGAATTGGTT TGGACCAAGT 
CCTTATGTAG AAGTCACAGT AGATGGGCAG TCAAAGAAGA CAGAAAAATG CAACAATACA 
AACAGCCCCA AGTGGAAGCA GCCCCTCACA GTTATCGTTA CCCCTATGAG TAAATTATGT 
TTTCGTGTGT GGAGTCACCA GACCCTGAAA TCTGATGTTT TATTGGGAAC TGCTGGATTA 
GATATTTATG AAACATTAAA GTCAAACAAT ATGAAACTTG AGGAAGTAGT TATGACTTTG 
CAGCTTGTAG GTGACAAAGA GCCAACAGAG ACAATGGGAG ATTTGTCAGT TTGTCTTGAT 
GGGCTGCAAG TAGAAGCTGA AGTTGTTACT AATGGTGAAA CTTCATGCTC AGAGGGTGCT 
ACACAGAATG ACGCTGGCTG CAGACCCAAA GATGAAACAA GAGTGAGCAC AAATGGATCA 
GAGGACCCTG AGGTTGCAGC CTCGGGGGAA AACAAGAGGG CCAATGGGAA CAATTCTCCA 
TCCCTTTCAA ATGGTGGTTT TAAGCCTTCT AGACCTCCTA GACCTTCAAG ACCACCTCCA 
CCCACCCCAC GAAGACCAGC TTCTGTCAAT GGCTCACCAT CCACGAACTC TGACAGCGAT 
GGATCTAGCA CAGGCTCTTT GCCACCCACA AATACAAATG TAAATTCAAG TGCATCGGAA 
GGAGCAGCAT CTGGGTTAAT AATTCCTCTG ACCATCTCTG GGGGCTCAGG CCCTAGGCCG 
CTGAATACTG TCAGTCAAGC TCCCCTCCCA CCTGGGTGGG AGCAGAGAGT AGATCAGCAT 
GGGCGTGTTT ACTATGTAGA CCATGTTGAA AAGCGAACAA CATGGGATAG ACCGGAACCT 
CTACCTCCTG GCTGGGAACG GCGTGTGGAC AATATGGGAC GTATTTATTA TGTTGACCAT 
TTCACGAGAA CCACAACATG GCAGAGGCCG ACCTTGGAAT CTGTCCGGAA CTATGAGCAG 
TGGCAGCTCC AGCGTAGTCA ACTTCAGGGA GCAATGCAGC AGTTTAACCA GAGATTCATC 
TTTGGGAATC AAGATTTGTT TGCTACATCA CAAAACAAAG AGTTTGATCC CCTTGGTCCT 
TTGCCCCCTG GATGGGAGAA GAGAACTGAT AGCAACGGCA GAGTGTATTT TGTCAACCAC 
AACACTCGGA TTACACAGTG GGAAGACCCC AGAAGCCAAG GTCAGTTAAA TGAAAAGCCC 
TTACCAGAAG GCTGGGAAAT GCGATTCACA GTGGATGGAA TTCCATATTT TGTGGACCAC 
AATAGAAGAG CAACTACCTA CATAGATCCA CGCACAGGAA AGTCAGCCTT AGACAACGGA 
CCCCAGATAG CCTATGTTCG GGACTTCAAG GCAAAAGTTC AGTATTTCCG GTTCTGGTGT 
CAGCAACTGG CCATGCCACA GCATATAAAG ATCACAGTGA CAAGAAAAAC ATTGTTTGAG 
GATTCCTTTC AACAGATCAT GAGCTTCAGT CCGCAAGATC TGAGAAGACG TTTGTGGGTG 
ATTTTCCCAG GAGAAGAAGG TTTAGATTAT GGAGGTGTAG CAAGAGAATG GTTCTTTCTT 
TTGTCACATG AAGTGTTGAA CCCAATGTAT TGCCTGTTTG AATATGCAGG GAAGGATAAC 
TACTGCCTGC AGATAAACCC CGCTTCTTAT ATCAACCCAG ACCACCTGAA ATACTTCCGT 
TTTATTGGCA GATTTATTGC CATGGCTCTA TTCCATGGGA AATTCATAGA TACCGGATTT 
TCTTTACCAT TCTATAAACG TATCTTGAAT AAACCAGTGG GACTTAAGGA TTTAGAATCT 
ATCGATCCAG AATTTTACAA TTCTCTCATC TGGGTTAAAG AAAACAATAT CGAGGAATGT 
GGTTTGGAAA TGTACTTCTC AGTTGATAAA GAAATTCTGG GTGAAATTAA GAGTCATGAT 
TTGAAACCCA ATGGTGGCAA TATTCTGGTG ACAGAAGAAA ATAAGGAAGA GTACATCAGG 
ATGGTCGCTG AGTGGAGGTT GTCTCGGGGA GTTGAAGAGC AGACACAAGC TTTCTTTGAG 
GGCTTTAATG AAATTCTTCC TCAGCAATAT TTGCAATATT TTGATGCGAA GGAATTAGAG 
GTCCTTTTGT GTGGGATGCA GGAGATCGAT TTGAATGACT GGCAGAGACA CGCCATCTAC 
CGTCACTATA CCAGGACGAG CAAGCAGATC GTGTGGTTTT GGCAGTTTGT TAAAGAAATT 
GATAACGAGA AGAGGATGCG ACTTCTGCAG TTTGTTACCG GAACCTGCCG ATTGCCAGTG 
GGAGGATTTG CTGACCTCAT GGGGAGTAAT GGACCACAGA AGTTCTGCAT CGAAAAAGTT 
GGCAAAGAAA ATTGGTTACC CAGAAGCCAT ACTTGTTTTA ACCGCCTGGA CCTCCCACCC 
TACAAGAGCT ATGAGCAACT GAAGGAAAAG CTGTTATTTG CCATTGAAGA AACAGAAGGA 
TTTGGACAAG AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

HECT-Type Ubiquitin E3 Ligase ITCH Interacts With Thioredoxin-Interacting Protein and Ameliorates Reactive Oxygen Species-Induced Cardiotoxicity.
Journal of the American Heart Association5(1)(2016 Jan)
Otaki Y,Takahashi H,Watanabe T,Funayama A,Netsu S,Honda Y,Narumi T,Kadowaki S,Hasegawa H,Honda S,Arimoto T,Shishido T,Miyamoto T,Kamata H,Nakajima O,Kubota I

Nedd4 family interacting protein 1 (Ndfip1) is required for ubiquitination and nuclear trafficking of BRCA1-associated ATM activator 1 (BRAT1) during the DNA damage response.
The Journal of biological chemistry290(11)7141-50(2015 Mar)
Low LH,Chow YL,Li Y,Goh CP,Putz U,Silke J,Ouchi T,Howitt J,Tan SS

The ubiquitin ligase deltex-3l regulates endosomal sorting of the G protein-coupled receptor CXCR4.
Molecular biology of the cell25(12)1892-904(2014 Jun)
Holleman J,Marchese A

?-arrestin 1 (ARRDC1) and ?-arrestins cooperate to mediate Notch degradation in mammals.
Journal of cell science126(Pt 19)4457-68(2013 Oct)
Puca L,Chastagner P,Meas-Yedid V,Isra?l A,Brou C

Regulation of NF-?B by ubiquitination.
Current opinion in immunology25(1)4-12(2013 Feb)
Chen J,Chen ZJ

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Itch cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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