gRNAs
The following Spg11 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Spg11 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
| CloneID | ORa60399 | |
| Clone ID Related Accession (Same CDS sequence) | XM_017591740.1 | |
| Accession Version | XM_017591740.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 7305bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector |  User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2016-07-25 | |
| Organism | Rattus norvegicus(Norway rat) | |
| Product | spatacsin isoform X1 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005102.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGGCTGCCA AGCCAGGGCC CGGAAGTGCT GCCTCCCCCG GCTGTGCCGG CGCCATGGAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_017447229.1 |
| CDS | 50..7354 |
| Translation |
Target ORF information:
Target ORF information:
|
 XM_017591740.1 |
1 | ATGGCTGCCA AGCCAGGGCC CGGAAGTGCT GCCTCCCCCG GCTGTGCCGG CGCCATGGAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | ORa60400 | |
| Clone ID Related Accession (Same CDS sequence) | XM_006234855.3 | |
| Accession Version | XM_006234855.3 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 7287bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2016-07-25 | |
| Organism | Rattus norvegicus(Norway rat) | |
| Product | spatacsin isoform X2 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005102.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006234855.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGGCTGCCA AGCCAGGGCC CGGAAGTGCT GCCTCCCCCG GCTGTGCCGG CGCCATGGAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_006234917.2 |
| CDS | 50..7336 |
| Translation |
Target ORF information:
Target ORF information:
|
XM_006234855.3 |
1 | ATGGCTGCCA AGCCAGGGCC CGGAAGTGCT GCCTCCCCCG GCTGTGCCGG CGCCATGGAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | ORa07025 | |
| Clone ID Related Accession (Same CDS sequence) | NM_001191571.1 | |
| Accession Version | NM_001191571.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 6948bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-04-08 | |
| Organism | Rattus norvegicus(Norway rat) | |
| Product | spatacsin | |
| Comment | Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AC112350.4. On Jul 17, 2010 this sequence version replaced XM_002729179.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA2689601, SAMEA2689603 [ECO:0000350] ##Evidence-Data-END## | |
1 | ATGGCTGCCA AGCCAGGGCC CGGAAGTGCT GCCTCCCCCG GCTGTGCCGG CGCCATGGAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_001178500.1 |
| CDS | 1..6948 |
| Translation |
Target ORF information:
Target ORF information:
|
NM_001191571.1 |
1 | ATGGCTGCCA AGCCAGGGCC CGGAAGTGCT GCCTCCCCCG GCTGTGCCGG CGCCATGGAG |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
In Vivo Evidence for Lysosome Depletion and Impaired Autophagic Clearance in Hereditary Spastic Paraplegia Type SPG11. |
Dysfunction of spatacsin leads to axonal pathology in SPG11-linked hereditary spastic paraplegia. |
Cellular distribution and subcellular localization of spatacsin and spastizin, two proteins involved in hereditary spastic paraplegia. |
Integral and associated lysosomal membrane proteins. |
Mutations in SPG11, encoding spatacsin, are a major cause of spastic paraplegia with thin corpus callosum. |
