Ext2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ext2
Entrez Gene ID	311215
Full Name	exostosin glycosyltransferase 2
General protein information	Preferred Names exostosin glycosyltransferase 2 Names exostosin-2 exostoses (multiple) 2 exostosin 2
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 3 Map Location: 3q31

mRNA and Protein(s)

mRNA	Protein	Name
NM_001107751.1	NP_001101221.1	exostosin-2
XM_006234576.3	XP_006234638.2	exostosin-2 isoform X1

Pathways from BioSystems

Homologies

Drosophila melanogaster (fruit fly)	Ext2	NP_725536.1
Canis lupus familiaris (dog)	EXT2	XP_005631368.1
Rattus norvegicus (Norway rat)	Ext2	NP_001101221.1
Danio rerio (zebrafish)	ext2	NP_001008400.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000081	XP_311070.5
Homo sapiens (human)	EXT2	NP_001171554.1
Pan troglodytes (chimpanzee)	EXT2	XP_003313041.1
	EXT2	XP_001106468.2
Gallus gallus (chicken)	EXT2	NP_001026520.1
Xenopus tropicalis (tropical clawed frog)	ext2	NP_001120887.1
Bos taurus (cattle)	EXT2	NP_803462.2
Mus musculus (house mouse)	Ext2	NP_034293.2

Gene Ontology
Bibliography

Related articles in PubMed

Expression of Ext1, Ext2, and heparanase genes in brain of senescence-accelerated OXYS rats in early ontogenesis and during development of neurodegenerative changes.
Shevelev OB, Rykova VI, Fedoseeva LA, Leberfarb EY, Dymshits GM, Kolosova NG
Biochemistry. Biokhimiia77(1)56-61(2012 Jan)

Defining the membrane proteome of NK cells.
Ghosh D, Lippert D, Krokhin O, Cortens JP, Wilkins JA
Journal of mass spectrometry : JMS45(1)1-25(2010 Jan)

Proteomic analysis of human parotid gland exosomes by multidimensional protein identification technology (MudPIT).
Gonzalez-Begne M, Lu B, Han X, Hagen FK, Hand AR, Melvin JE, Yates JR
Journal of proteome research8(3)1304-14(2009 Mar)

Contribution of EXT1, EXT2, and EXTL3 to heparan sulfate chain elongation.
Busse M, Feta A, Presto J, Wil?n M, Gr?nning M, Kjell?n L, Kusche-Gullberg M
The Journal of biological chemistry282(45)32802-10(2007 Nov)

Mice deficient in Ext2 lack heparan sulfate and develop exostoses.
Stickens D, Zak BM, Rougier N, Esko JD, Werb Z
Development (Cambridge, England)132(22)5055-68(2005 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Ext2 gene is involved in heparan sulfate proteoglycans metabolism.
Title: Expression of Ext1, Ext2, and heparanase genes in brain of senescence-accelerated OXYS rats in early ontogenesis and during development of neurodegenerative changes.

The following Ext2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ext2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa05852	NM_001107751.1 Latest version!	Rattus norvegicus exostosin glycosyltransferase 2 (Ext2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
ORa46965	XM_006234576.3 Latest version!	Rattus norvegicus exostosin glycosyltransferase 2 (Ext2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$391.30 $559.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ext2 ( NM_001107751.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001101221.1 Rattus norvegicus exostosin glycosyltransferase 2 (Ext2), mRNA.

Price & Availability↑

CloneID	ORa05852
Clone ID Related Accession (Same CDS sequence)	NM_001107751.1
Accession Version	NM_001107751.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2013bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Rattus norvegicus(Norway rat)
Product	exostosin-2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CH473949.1. On or before Oct 4, 2007 this sequence version replaced XM_230303.4, XM_001078606.1. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGTGTGCGT CCGTCAAGTC CAACATCCGG GGTCCTGCCC TCATCCCAAG GATGAAGACC 
AAGCACCGGA TCTACTACGT CACCCTGTTC TCCATCGTCC TCCTGGGCCT CATCGCCACT 
GGCATGTTTC AGTTCTGGCC TCACTCCATC GAGTCCTCCA GCGATGGGGG TGTGGAGAAA 
CGCAGCATCC GGGAAGTGCC AGTGGTCAGG CTGACAGCTG ACAGCCCTAT CCCAGAGCGG 
GGGGATCTCA GCTGTAGAAT GCACACGTGT TTCGATGTCT ACCGCTGCGG CTTCAACCCA 
AAGAACAAAA TCAAGGTGTA CATCTACCCC CTAAAGAAGT ACGTGGATGA CGCCGGTGTT 
CCGGTGAGCA GCGCCATCTC CCGGGAGTAC AATGAACTGC TCACAGCCAT CTCAGACAGT 
GACTACTACA CAGATGACAT CAGCCGAGCC TGTCTGTTCG TCCCCTCCAT TGATGTGCTG 
AACCAGAACC CCCTTCGAAT CAAGGAGACA GCGCAAGCCC TGGCCCAGCT TCCCAGGTGG 
GATCGAGGGA CAAATCACCT GCTGTTCAAC ATGTTGCCTG GAGGTCCTCC AGATTACAAC 
ACTGCCCTGG ATGTCCCCAG AGACAGGGCA CTGCTGGCTG GTGGCGGCTT TTCTACCTGG 
ACTTACCGGC AGGGCTACGA TGTCAGCATT CCTGTTTTCA GCCCACTGTC AGCCGAAGTG 
GCTCTCCCAG AGAAAGCACC AGGTTCCCGG CGATACTTCC TGCTGTCCTC TCAGATGGCC 
ATCCACCCTG AGTACAGAGA GGAACTAGAA GCCCTCCAGG CCAAACACAG AGAGTCCGTG 
TTAGTCCTAG ACAAATGCAC CAATCTCTCA GAGGGCGTCC TTTCCATCCG AAAGCGCTGC 
CACCAGCACC AGGTCTTTGA TTACCCCCAA GTACTGCAGG AGGCTACTTT TTGTACGGTT 
CTTCGAGGAG CTCGGCTGGG CCAGGCAGTG CTGAGCGATG TCTTACAGGC TGGCTGTGTC 
CCAGTTGTCA TTGCAGACTC TTACATTCTG CCTTTCTCTG AAGTTCTGGA CTGGAAGAGG 
GCATCTGTGG TCATTCCAGA GGAGAAGATG TCAGATGTGT ACAGCATCCT GCAGAACATC 
CCACAGAGGC AGATTGAAGA GATGCAGAGA CAGGCACGAT GGTTCTGGGA GGCATACTTT 
CGGTCCATTA AAGCCATCGC CCTGGCCACC CTACAGATCA TCAATGACAG GATCTATCCA 
TATGCAGCCA TTTCCTATGA AGAGTGGAAT GACCCTCCTG CTGTGAAGTG GGCTAGTGTG 
AGCAACCCGC TCTTCCTCCC ACTGATCCCA CCACAGTCGC AAGGCTTCAC TGCCATTGTC 
CTCACCTATG ACCGTGTGGA GAGCCTCTTC CGGGTCATCA CCGAGGTGTC CAAGGTGCCC 
AGTCTGTCCA AGCTGCTGGT GGTCTGGAAT AATCAGAATA AAAACCCTCC AGAAGAATCT 
CTGTGGCCCA AAATCCGTGT TCCCCTGAAG GTTGTGAGGA CTGCAGAAAA CAAGCTAAGT 
AATCGTTTCT TCCCTTACGA TGAAATCGAG ACAGAGGCCG TGCTGGCCAT CGATGATGAT 
ATCATCATGC TGACCTCTGA TGAGCTGCAG TTTGGTTATG AGTATTTTAA TTACCTGTAT 
ACCTACAAAA TGCCTGGGGA CATCAAGAAC TGGGTAGATG CTCACATGAA CTGTGAAGAT 
ATTGCCATGA ATTTCCTAGT GGCCAATGTC ACAGGGAAAG CTGTCATCAA GGTAACCCCA 
AGAAAGAAGT TCAAGTGTCC TGAGTGCACA GCCATCGACG GACTCTCACT AGACCAGACT 
CACATGGTAG AGAGGTCTGA GTGCATCAAT AAGTTTGCTT CCGTCTTCGG AACGATGCCC 
CTCAAGGTGG TGGAGCACCG AGCAGACCCT GTACTCTACA AAGATGACTT CCCTGAGAAA 
CTGAAGAGTT TCCCCAACAT CGGCAGCTTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001101221.1
CDS	204..2216
Translation	MCASVKSNIRGPALIPRMKTKHRIYYVTLFSIVLLGLIATGMFQFWPHSIESSSDGGVEKRSIREVPVVRLTADSPIPERGDLSCRMHTCFDVYRCGFNPKNKIKVYIYPLKKYVDDAGVPVSSAISREYNELLTAISDSDYYTDDISRACLFVPSIDVLNQNPLRIKETAQALAQLPRWDRGTNHLLFNMLPGGPPDYNTALDVPRDRALLAGGGFSTWTYRQGYDVSIPVFSPLSAEVALPEKAPGSRRYFLLSSQMAIHPEYREELEALQAKHRESVLVLDKCTNLSEGVLSIRKRCHQHQVFDYPQVLQEATFCTVLRGARLGQAVLSDVLQAGCVPVVIADSYILPFSEVLDWKRASVVIPEEKMSDVYSILQNIPQRQIEEMQRQARWFWEAYFRSIKAIALATLQIINDRIYPYAAISYEEWNDPPAVKWASVSNPLFLPLIPPQSQGFTAIVLTYDRVESLFRVITEVSKVPSLSKLLVVWNNQNKNPPEESLWPKIRVPLKVVRTAENKLSNRFFPYDEIETEAVLAIDDDIIMLTSDELQFGYEYFNYLYTYKMPGDIKNWVDAHMNCEDIAMNFLVANVTGKAVIKVTPRKKFKCPECTAIDGLSLDQTHMVERSECINKFASVFGTMPLKVVEHRADPVLYKDDFPEKLKSFPNIGSL

Target ORF information:

RefSeq Version	NM_001107751.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus exostosin glycosyltransferase 2 (Ext2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001107751.1

ORF Insert Sequence:

ATGTGTGCGT CCGTCAAGTC CAACATCCGG GGTCCTGCCC TCATCCCAAG GATGAAGACC 
AAGCACCGGA TCTACTACGT CACCCTGTTC TCCATCGTCC TCCTGGGCCT CATCGCCACT 
GGCATGTTTC AGTTCTGGCC TCACTCCATC GAGTCCTCCA GCGATGGGGG TGTGGAGAAA 
CGCAGCATCC GGGAAGTGCC AGTGGTCAGG CTGACAGCTG ACAGCCCTAT CCCAGAGCGG 
GGGGATCTCA GCTGTAGAAT GCACACGTGT TTCGATGTCT ACCGCTGCGG CTTCAACCCA 
AAGAACAAAA TCAAGGTGTA CATCTACCCC CTAAAGAAGT ACGTGGATGA CGCCGGTGTT 
CCGGTGAGCA GCGCCATCTC CCGGGAGTAC AATGAACTGC TCACAGCCAT CTCAGACAGT 
GACTACTACA CAGATGACAT CAGCCGAGCC TGTCTGTTCG TCCCCTCCAT TGATGTGCTG 
AACCAGAACC CCCTTCGAAT CAAGGAGACA GCGCAAGCCC TGGCCCAGCT TCCCAGGTGG 
GATCGAGGGA CAAATCACCT GCTGTTCAAC ATGTTGCCTG GAGGTCCTCC AGATTACAAC 
ACTGCCCTGG ATGTCCCCAG AGACAGGGCA CTGCTGGCTG GTGGCGGCTT TTCTACCTGG 
ACTTACCGGC AGGGCTACGA TGTCAGCATT CCTGTTTTCA GCCCACTGTC AGCCGAAGTG 
GCTCTCCCAG AGAAAGCACC AGGTTCCCGG CGATACTTCC TGCTGTCCTC TCAGATGGCC 
ATCCACCCTG AGTACAGAGA GGAACTAGAA GCCCTCCAGG CCAAACACAG AGAGTCCGTG 
TTAGTCCTAG ACAAATGCAC CAATCTCTCA GAGGGCGTCC TTTCCATCCG AAAGCGCTGC 
CACCAGCACC AGGTCTTTGA TTACCCCCAA GTACTGCAGG AGGCTACTTT TTGTACGGTT 
CTTCGAGGAG CTCGGCTGGG CCAGGCAGTG CTGAGCGATG TCTTACAGGC TGGCTGTGTC 
CCAGTTGTCA TTGCAGACTC TTACATTCTG CCTTTCTCTG AAGTTCTGGA CTGGAAGAGG 
GCATCTGTGG TCATTCCAGA GGAGAAGATG TCAGATGTGT ACAGCATCCT GCAGAACATC 
CCACAGAGGC AGATTGAAGA GATGCAGAGA CAGGCACGAT GGTTCTGGGA GGCATACTTT 
CGGTCCATTA AAGCCATCGC CCTGGCCACC CTACAGATCA TCAATGACAG GATCTATCCA 
TATGCAGCCA TTTCCTATGA AGAGTGGAAT GACCCTCCTG CTGTGAAGTG GGCTAGTGTG 
AGCAACCCGC TCTTCCTCCC ACTGATCCCA CCACAGTCGC AAGGCTTCAC TGCCATTGTC 
CTCACCTATG ACCGTGTGGA GAGCCTCTTC CGGGTCATCA CCGAGGTGTC CAAGGTGCCC 
AGTCTGTCCA AGCTGCTGGT GGTCTGGAAT AATCAGAATA AAAACCCTCC AGAAGAATCT 
CTGTGGCCCA AAATCCGTGT TCCCCTGAAG GTTGTGAGGA CTGCAGAAAA CAAGCTAAGT 
AATCGTTTCT TCCCTTACGA TGAAATCGAG ACAGAGGCCG TGCTGGCCAT CGATGATGAT 
ATCATCATGC TGACCTCTGA TGAGCTGCAG TTTGGTTATG AGTATTTTAA TTACCTGTAT 
ACCTACAAAA TGCCTGGGGA CATCAAGAAC TGGGTAGATG CTCACATGAA CTGTGAAGAT 
ATTGCCATGA ATTTCCTAGT GGCCAATGTC ACAGGGAAAG CTGTCATCAA GGTAACCCCA 
AGAAAGAAGT TCAAGTGTCC TGAGTGCACA GCCATCGACG GACTCTCACT AGACCAGACT 
CACATGGTAG AGAGGTCTGA GTGCATCAAT AAGTTTGCTT CCGTCTTCGG AACGATGCCC 
CTCAAGGTGG TGGAGCACCG AGCAGACCCT GTACTCTACA AAGATGACTT CCCTGAGAAA 
CTGAAGAGTT TCCCCAACAT CGGCAGCTTG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ext2 ( XM_006234576.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006234638.2 Rattus norvegicus exostosin glycosyltransferase 2 (Ext2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa46965
Clone ID Related Accession (Same CDS sequence)	XM_006234576.3
Accession Version	XM_006234576.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2376bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	exostosin-2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005102.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006234576.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGCAGTG CGCGCCGTGT CAGACGTGGG GAACTGGAGG CCGGCGCAGC GTTCCCCGGC 
TGGCTCTGGC TTACCTGGTG CTGGCGGCGG CCTCCCGTCA CCGCGCGATC CGACACGGCC 
GTGGCTCGCG CCCCGCGCCC AAAGCCGGTG GCGGAGCGCG GGGCCGAGCG GGAGGCGGCC 
GGTTCCGAGG TGTGTGAGGA CGAGGACATC TGTGTCATTA TGTGTGCGTC CGTCAAGTCC 
AACATCCGGG GTCCTGCCCT CATCCCAAGG ATGAAGACCA AGCACCGGAT CTACTACGTC 
ACCCTGTTCT CCATCGTCCT CCTGGGCCTC ATCGCCACTG GCATGTTTCA GTTCTGGCCT 
CACTCCATCG AGTCCTCCAG CGATGGGGGT GTGGAGAAAC GCAGCATCCG GGAAGTGCCA 
GTGGTCAGGC TGACAGCTGA CAGCCCTATC CCAGAGCGGG GGGATCTCAG CTGTAGAATG 
CACACGTGTT TCGATGTCTA CCGCTGCGGC TTCAACCCAA AGAACAAAAT CAAGGTGTAC 
ATCTACCCCC TAAAGAAGTA CGTGGATGAC GCCGGTGTTC CGGTGAGCAG CGCCATCTCC 
CGGGAGTACA ATGAACTGCT CACAGCCATC TCAGACAGTG ACTACTACAC AGATGACATC 
AGCCGAGCCT GTCTGTTCGT CCCCTCCATT GATGTGCTGA ACCAGAACCC CCTTCGAATC 
AAGGAGACAG CGCAAGCCCT GGCCCAGCTT CCCAGGTGGG ATCGAGGGAC AAATCACCTG 
CTGTTCAACA TGTTGCCTGG AGGTCCTCCA GATTACAACA CTGCCCTGGA TGTCCCCAGA 
GACAGGGCAC TGCTGGCTGG TGGCGGCTTT TCTACCTGGA CTTACCGGCA GGGCTACGAT 
GTCAGCATTC CTGTTTTCAG CCCACTGTCA GCCGAAGTGG CTCTCCCAGA GAAAGCACCA 
GGTTCCCGGC GATACTTCCT GCTGTCCTCT CAGATGGCCA TCCACCCTGA GTACAGAGAG 
GAACTAGAAG CCCTCCAGGC CAAACACAGA GAGTCCGTGT TAGTCCTAGA CAAATGCACC 
AATCTCTCAG AGGGCGTCCT TTCCATCCGA AAGCGCTGCC ACCAGCACCA GGTCTTTGAT 
TACCCCCAAG TACTGCAGGA GGCTACTTTT TGTACGGTTC TTCGAGGAGC TCGGCTGGGC 
CAGGCAGTGC TGAGCGATGT CTTACAGGCT GGCTGTGTCC CAGTTGTCAT TGCAGACTCT 
TACATTCTGC CTTTCTCTGA AGTTCTGGAC TGGAAGAGGG CATCTGTGGT CATTCCAGAG 
GAGAAGATGT CAGATGTGTA CAGCATCCTG CAGAACATCC CACAGAGGCA GATTGAAGAG 
ATGCAGAGAC AGGCACGATG GTTCTGGGAG GCATACTTTC GGTCCATTAA AGCCATCGCC 
CTGGCCACCC TACAGATCAT CAATGACAGG ATCTATCCAT ATGCAGCCAT TTCCTATGAA 
GAGTGGAATG ACCCTCCTGC TGTGAAGTGG GCTAGTGTGA GCAACCCGCT CTTCCTCCCA 
CTGATCCCAC CACAGTCGCA AGGCTTCACT GCCATTGTCC TCACCTATGA CCGTGTGGAG 
AGCCTCTTCC GGGTCATCAC CGAGGTGTCC AAGGTGCCCA GTCTGTCCAA GCTGCTGGTG 
GTCTGGAATA ATCAGAATAA AAACCCTCCA GAAGAATCTC TGTGGCCCAA AATCCGTGTT 
CCCCTGAAGG TTGTGAGGAC TGCAGAAAAC AAGCTAAGTA ATCGTTTCTT CCCTTACGAT 
GAAATCGAGA CAGAGGCCGT GCTGGCCATC GATGATGATA TCATCATGCT GACCTCTGAT 
GAGCTGCAGT TTGGTTATGA GGTCTGGCGG GAATTTCCTG ACCGGCTGGT GGGCTACCCT 
GGCCGCCTGC ATCTCTGGGA CCACGAGATG AACAAGTGGA AGTATGAGTC TGAGTGGACC 
AATGAGGTAT CCATGGTGCT CACGGGTGCA GCCTTCTATC ACAAGTATTT TAATTACCTG 
TATACCTACA AAATGCCTGG GGACATCAAG AACTGGGTAG ATGCTCACAT GAACTGTGAA 
GATATTGCCA TGAATTTCCT AGTGGCCAAT GTCACAGGGA AAGCTGTCAT CAAGGTAACC 
CCAAGAAAGA AGTTCAAGTG TCCTGAGTGC ACAGCCATCG ACGGACTCTC ACTAGACCAG 
ACTCACATGG TAGAGAGGTC TGAGTGCATC AATAAGTTTG CTTCCGTCTT CGGAACGATG 
CCCCTCAAGG TGGTGGAGCA CCGAGCAGAC CCTGTACTCT ACAAAGATGA CTTCCCTGAG 
AAACTGAAGA GTTTCCCCAA CATCGGCAGC TTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006234638.2
CDS	9..2384
Translation	MRSARRVRRGELEAGAAFPGWLWLTWCWRRPPVTARSDTAVARAPRPKPVAERGAEREAAGSEVCEDEDICVIMCASVKSNIRGPALIPRMKTKHRIYYVTLFSIVLLGLIATGMFQFWPHSIESSSDGGVEKRSIREVPVVRLTADSPIPERGDLSCRMHTCFDVYRCGFNPKNKIKVYIYPLKKYVDDAGVPVSSAISREYNELLTAISDSDYYTDDISRACLFVPSIDVLNQNPLRIKETAQALAQLPRWDRGTNHLLFNMLPGGPPDYNTALDVPRDRALLAGGGFSTWTYRQGYDVSIPVFSPLSAEVALPEKAPGSRRYFLLSSQMAIHPEYREELEALQAKHRESVLVLDKCTNLSEGVLSIRKRCHQHQVFDYPQVLQEATFCTVLRGARLGQAVLSDVLQAGCVPVVIADSYILPFSEVLDWKRASVVIPEEKMSDVYSILQNIPQRQIEEMQRQARWFWEAYFRSIKAIALATLQIINDRIYPYAAISYEEWNDPPAVKWASVSNPLFLPLIPPQSQGFTAIVLTYDRVESLFRVITEVSKVPSLSKLLVVWNNQNKNPPEESLWPKIRVPLKVVRTAENKLSNRFFPYDEIETEAVLAIDDDIIMLTSDELQFGYEVWREFPDRLVGYPGRLHLWDHEMNKWKYESEWTNEVSMVLTGAAFYHKYFNYLYTYKMPGDIKNWVDAHMNCEDIAMNFLVANVTGKAVIKVTPRKKFKCPECTAIDGLSLDQTHMVERSECINKFASVFGTMPLKVVEHRADPVLYKDDFPEKLKSFPNIGSL

Target ORF information:

RefSeq Version	XM_006234576.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus exostosin glycosyltransferase 2 (Ext2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006234576.3

ORF Insert Sequence:

ATGCGCAGTG CGCGCCGTGT CAGACGTGGG GAACTGGAGG CCGGCGCAGC GTTCCCCGGC 
TGGCTCTGGC TTACCTGGTG CTGGCGGCGG CCTCCCGTCA CCGCGCGATC CGACACGGCC 
GTGGCTCGCG CCCCGCGCCC AAAGCCGGTG GCGGAGCGCG GGGCCGAGCG GGAGGCGGCC 
GGTTCCGAGG TGTGTGAGGA CGAGGACATC TGTGTCATTA TGTGTGCGTC CGTCAAGTCC 
AACATCCGGG GTCCTGCCCT CATCCCAAGG ATGAAGACCA AGCACCGGAT CTACTACGTC 
ACCCTGTTCT CCATCGTCCT CCTGGGCCTC ATCGCCACTG GCATGTTTCA GTTCTGGCCT 
CACTCCATCG AGTCCTCCAG CGATGGGGGT GTGGAGAAAC GCAGCATCCG GGAAGTGCCA 
GTGGTCAGGC TGACAGCTGA CAGCCCTATC CCAGAGCGGG GGGATCTCAG CTGTAGAATG 
CACACGTGTT TCGATGTCTA CCGCTGCGGC TTCAACCCAA AGAACAAAAT CAAGGTGTAC 
ATCTACCCCC TAAAGAAGTA CGTGGATGAC GCCGGTGTTC CGGTGAGCAG CGCCATCTCC 
CGGGAGTACA ATGAACTGCT CACAGCCATC TCAGACAGTG ACTACTACAC AGATGACATC 
AGCCGAGCCT GTCTGTTCGT CCCCTCCATT GATGTGCTGA ACCAGAACCC CCTTCGAATC 
AAGGAGACAG CGCAAGCCCT GGCCCAGCTT CCCAGGTGGG ATCGAGGGAC AAATCACCTG 
CTGTTCAACA TGTTGCCTGG AGGTCCTCCA GATTACAACA CTGCCCTGGA TGTCCCCAGA 
GACAGGGCAC TGCTGGCTGG TGGCGGCTTT TCTACCTGGA CTTACCGGCA GGGCTACGAT 
GTCAGCATTC CTGTTTTCAG CCCACTGTCA GCCGAAGTGG CTCTCCCAGA GAAAGCACCA 
GGTTCCCGGC GATACTTCCT GCTGTCCTCT CAGATGGCCA TCCACCCTGA GTACAGAGAG 
GAACTAGAAG CCCTCCAGGC CAAACACAGA GAGTCCGTGT TAGTCCTAGA CAAATGCACC 
AATCTCTCAG AGGGCGTCCT TTCCATCCGA AAGCGCTGCC ACCAGCACCA GGTCTTTGAT 
TACCCCCAAG TACTGCAGGA GGCTACTTTT TGTACGGTTC TTCGAGGAGC TCGGCTGGGC 
CAGGCAGTGC TGAGCGATGT CTTACAGGCT GGCTGTGTCC CAGTTGTCAT TGCAGACTCT 
TACATTCTGC CTTTCTCTGA AGTTCTGGAC TGGAAGAGGG CATCTGTGGT CATTCCAGAG 
GAGAAGATGT CAGATGTGTA CAGCATCCTG CAGAACATCC CACAGAGGCA GATTGAAGAG 
ATGCAGAGAC AGGCACGATG GTTCTGGGAG GCATACTTTC GGTCCATTAA AGCCATCGCC 
CTGGCCACCC TACAGATCAT CAATGACAGG ATCTATCCAT ATGCAGCCAT TTCCTATGAA 
GAGTGGAATG ACCCTCCTGC TGTGAAGTGG GCTAGTGTGA GCAACCCGCT CTTCCTCCCA 
CTGATCCCAC CACAGTCGCA AGGCTTCACT GCCATTGTCC TCACCTATGA CCGTGTGGAG 
AGCCTCTTCC GGGTCATCAC CGAGGTGTCC AAGGTGCCCA GTCTGTCCAA GCTGCTGGTG 
GTCTGGAATA ATCAGAATAA AAACCCTCCA GAAGAATCTC TGTGGCCCAA AATCCGTGTT 
CCCCTGAAGG TTGTGAGGAC TGCAGAAAAC AAGCTAAGTA ATCGTTTCTT CCCTTACGAT 
GAAATCGAGA CAGAGGCCGT GCTGGCCATC GATGATGATA TCATCATGCT GACCTCTGAT 
GAGCTGCAGT TTGGTTATGA GGTCTGGCGG GAATTTCCTG ACCGGCTGGT GGGCTACCCT 
GGCCGCCTGC ATCTCTGGGA CCACGAGATG AACAAGTGGA AGTATGAGTC TGAGTGGACC 
AATGAGGTAT CCATGGTGCT CACGGGTGCA GCCTTCTATC ACAAGTATTT TAATTACCTG 
TATACCTACA AAATGCCTGG GGACATCAAG AACTGGGTAG ATGCTCACAT GAACTGTGAA 
GATATTGCCA TGAATTTCCT AGTGGCCAAT GTCACAGGGA AAGCTGTCAT CAAGGTAACC 
CCAAGAAAGA AGTTCAAGTG TCCTGAGTGC ACAGCCATCG ACGGACTCTC ACTAGACCAG 
ACTCACATGG TAGAGAGGTC TGAGTGCATC AATAAGTTTG CTTCCGTCTT CGGAACGATG 
CCCCTCAAGG TGGTGGAGCA CCGAGCAGAC CCTGTACTCT ACAAAGATGA CTTCCCTGAG 
AAACTGAAGA GTTTCCCCAA CATCGGCAGC TTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Expression of Ext1, Ext2, and heparanase genes in brain of senescence-accelerated OXYS rats in early ontogenesis and during development of neurodegenerative changes.
Biochemistry. Biokhimiia77(1)56-61(2012 Jan)
Shevelev OB,Rykova VI,Fedoseeva LA,Leberfarb EY,Dymshits GM,Kolosova NG

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Ext2 cDNA ORF clone, Rattus norvegicus(Norway rat)

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ORF Insert Sequence:

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