Mttp cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Mttp
Entrez Gene ID	310900
Full Name	microsomal triglyceride transfer protein
Synonyms	MTP
General protein information	Preferred Names microsomal triglyceride transfer protein Names microsomal triglyceride transfer protein large subunit
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 2 Map Location: 2q44

mRNA and Protein(s)

mRNA	Protein	Name
NM_001107727.1	NP_001101197.1	microsomal triglyceride transfer protein large subunit precursor

Pathways from BioSystems

Homologies

Bos taurus (cattle)	MTTP	NP_001095304.1
Danio rerio (zebrafish)	mtp	NP_998135.1
Xenopus tropicalis (tropical clawed frog)	LOC100487453	XP_002934813.1
Pan troglodytes (chimpanzee)	MTTP	XP_001167562.1
Drosophila melanogaster (fruit fly)	Mtp	NP_610075.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010234	XP_319421.4
	MTTP	XP_001107709.1
Rattus norvegicus (Norway rat)	Mttp	NP_001101197.1
Gallus gallus (chicken)	MTTP	NP_001103254.1
Homo sapiens (human)	MTTP	NP_000244.2
Canis lupus familiaris (dog)	MTTP	XP_544995.3
Mus musculus (house mouse)	Mttp	NP_032668.2

Gene Ontology
Bibliography

Related articles in PubMed

?-Linolenic acid supplementation and exercise training reveal independent and additive responses on hepatic lipid accumulation in obese rats.
Miotto PM, Horbatuk M, Proudfoot R, Matravadia S, Bakovic M, Chabowski A, Holloway GP
American journal of physiology. Endocrinology and metabolism312(6)E461-E470(2017 Jun)

Novel Abetalipoproteinemia Missense Mutation Highlights the Importance of the N-Terminal ?-Barrel in Microsomal Triglyceride Transfer Protein Function.
Walsh MT, Iqbal J, Josekutty J, Soh J, Di Leo E, ?zaydin E, G?nd?z M, Tarugi P, Hussain MM
Circulation. Cardiovascular genetics8(5)677-87(2015 Oct)

Improvement in the expression of hepatic genes involved in fatty acid metabolism in obese rats supplemented with taurine.
Bonfleur ML, Borck PC, Ribeiro RA, Caetano LC, Soares GM, Carneiro EM, Balbo SL
Life sciences13515-21(2015 Aug)

Up-Regulation Mttp and Apob Gene Expression in Rat Liver is Related to Post-Lipectomy Hypertriglyceridemia.
Dettlaff-Pokora A, Sledzinski T, Swierczynski J
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology36(5)1767-77(2015)

Novel missense MTTP gene mutations causing abetalipoproteinemia.
Miller SA, Burnett JR, Leonis MA, McKnight CJ, van Bockxmeer FM, Hooper AJ
Biochimica et biophysica acta1842(10)1548-54(2014 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data suggest that Mttp content of liver microsomes can be altered by dietary factors; here, Mttp is up-regulated in liver microsomes of obese rats with dietary supplementation of the fatty acid alpha-linolenic acid; animal physical conditioning (exercise) appears to up-regulate Mttp in liver microsomes in an independent manner.
Title: ?-Linolenic acid supplementation and exercise training reveal independent and additive responses on hepatic lipid accumulation in obese rats.

Tau supplementation prevented obesity and hepatic TG deposition by upregulating MTP mRNA, ameliorating hepatic lipid efflux, and consequently enhancing PPAR-alpha which increases lipid oxidation through ACO and CPT-1a gene expressions.
Title: Improvement in the expression of hepatic genes involved in fatty acid metabolism in obese rats supplemented with taurine.

Coordinated up-regulation of Mttp, Apob, Hnf1a, Hnf4a, Fasn, Gpam and Dgat (1 and 2) gene expressions may contribute to the increase in circulating and liver concentrations of TAGs after lipectomy in an experimental rat model
Title: Up-Regulation Mttp and Apob Gene Expression in Rat Liver is Related to Post-Lipectomy Hypertriglyceridemia.

As the XBP-1/PDI/MTP axis has been suggested to represent a significant lipogenic mechanism in the liver response to ER stress, our data indicate that hypertriglyceridemia and liver steatosis occurring MSG rats are associated with increased MTP.
Title: Mechanisms underlying hypertriglyceridemia in rats with monosodium L-glutamate-induced obesity: evidence of XBP-1/PDI/MTP axis activation.

Octreotide intervention down-regulated expression of apoB mRNA and apoB48 protein, as well as MTP mRNA and proteins. ApoAIV mRNA was not significantly different between obese and octreotide-treated rats.
Title: Octreotide promotes weight loss via suppression of intestinal MTP and apoB48 expression in diet-induced obesity rats.

The following Mttp gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Mttp cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa12907	NM_001107727.1 Latest version!	Rattus norvegicus microsomal triglyceride transfer protein (Mttp), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$524.30 $749.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Mttp ( NM_001107727.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001101197.1 Rattus norvegicus microsomal triglyceride transfer protein (Mttp), mRNA.

Price & Availability↑

CloneID	ORa12907
Clone ID Related Accession (Same CDS sequence)	NM_001107727.1
Accession Version	NM_001107727.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2691bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Rattus norvegicus(Norway rat)
Product	microsomal triglyceride transfer protein large subunit precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CH473952.1. On or before Oct 4, 2007 this sequence version replaced XM_227765.4, XM_001076172.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGATCCTCT TAGCTGTGCT TTTCCTCTGT TTCTTCTCCT CGTATTCTGC TTCTGTCAAA 
GGTCACACAA CTGGCCTCTC ATTAAATAAT GAGCGGCTAT ACAAGCTCAC GTACTCCACC 
GAAGTGTTTC TCGATGGGGG CAAAGGAAAA CTGCAGGACA GCGTGGGCTA CCGGATCTCA 
TCGGACGTGG ACGTTGTGTT ACTGTGGAGG AATCCCGACG GTGACGATGA TCAACTGATC 
CAAGTCACGA TAACGGCTGT CAATGTCGAA AATGCGGGTC AGCAGAGAGG AGAGAAGAGC 
ATCTTCAAGG GCAAAAGTAC ACCTAAGATC GTAGGAAAGG ACAACTTGGA GGCTCTACGG 
AGACCCATGC TTCTTCATCT GGTCCGGGGG AAGGTCAAAG AGTTCTACTC CTATGAAAAT 
GAGCCCGTGG GCATAGAAAA TCTCAAGAGA GGCTTGGCTA GCTTATTCCA GATGCAGTTA 
ACCTCTGGAA CCACCAATGA GGTAGATATC TCTGGGGATT GTAAAGTCAC CTACCAGGCT 
CAGCAAGACA AAGTGGTCAA AATTAAGGCT CTGGATACAT GCAAAATTGA GAGGTCCGGA 
TTTACAACAG CAAATCAGGT GCTGGGTGTC ACTTCAAAAG CCACCTCTGT CACTACCTAT 
AAGATAGAGG ACAGCTTTGT CACAGCAGTG GTTGCAGAAG AGACCAGGGC TTTTGCCTTG 
AACTTCCTAC AAACCGTGGC AGGAAAAATA GTGTCGAAGC AGAAATTGGA GCTGAAGACA 
ACGGAAGCCG GCCCAAGAAT GGTACCTGGG AAGCAAGTGG CAGGAGTAAT TAAGGCAATT 
GATTCCAAGT ACAAAGCCAT TCCCATTGTG GGACAGGTCC TCCAGAGTGT CTGTAAAGGC 
TGTCCTTCTC TGGCGGAGCA CTGGCAATCT ATCAGGAAGC ACCTAGAACC TGAGAACCTG 
TCCAACGCCG AGGCCGTCCA GAGCTTCCTG GCCTTCATTC AGCACCTCCG CACTTCGAGG 
AGAGAAGAGA TCCTTCAGAT TCTGAAGGCA GAGAAGAAAG AAGTTCTCCC TCAGCTGGTG 
GACGCCGTCA CCTCTGCTCA GACTCCAGCC TCACTGGAAG CCATCCTGGA CTTTTTGGAT 
TTCAAAAGCG ACAGCAGTAT CATTCTCCAG GAAAGGTTCC TCTATGCATG TGGATTTGCC 
TCCCATCCTG ATGAAGAACT CCTGCAAGCC CTCCTTAGCA AGTTCAAAGG TTCCTTTGCA 
AGCAACGACA TCAGAGAGTC GGTTATGATC ATCATTGGAG CCCTAGTCAG GAAGCTGTGT 
CAGAATGAAG GCTGCAAGCT CAAGGCAGTG GTTGAAGCTA AGAAGCTAAT CCTGGGAGGA 
CTTGAAAAAC CAGAGAAGAA AGAGGACACC ACGATGTACC TGCTGGCTCT GAAGAACGCC 
CTGCTTCCTG AAGGCATCCC GCTCCTTTTG AAGTACGCTG AGGCTGGGGA AGGGCCCGTC 
AGTCACCTGG CTACCACTGT TCTCCAGAGA TACGATGCCT CCTTCATCAC AGACGAGGTG 
AAGAAGACCT TGAACAGGAT ATACCATCAG AATCGTAAGG TTCATGAAAA GACTGTACGC 
ACAACGGCTG CTGCTGTCAT CTTAAAGAAC AACCCCTCCT ACATGGATGT GAAGAACATT 
CTGCTGTCTA TTGGGGAACT TCCTAAAGAA ATGAACAAAT ACATGCTCAC CATCGTGCAA 
GACATCCTGC ATTTCGAAAT GCCTGCAAGC AAAATGATCC GTCGGGTTCT CAAGGAGATG 
ATTGTTCACA ATTATGACCG TTTCTCCAAG AGTGGATCCT CTTCTGCCTA CACTGGCTAC 
GTAGAACGCA GTCCCCACGC GGCGTCCACC TACAGCCTTG ATATCCTCTA CTCTGGGTCT 
GGCATTCTGA GGAGAAGTAA CCTGAACATC TTCCAGTACA TCGGAAAGGC GGAGCTGCAT 
GGCAGCCAGG TGGTCATTGA AGCCCAAGGG CTGGAAGGCT TAATTGCAGC CACTCCAGAT 
GAGGGAGAGG AGAACCTTGA CTCGTATGCT GGTATGTCAG CCATCCTGTT TGATGTTCAG 
CTTAGACCTG TCACGTTCTT TAACGGATAC AGTGATTTGA TGTCCAAAAT GCTGTCGGCG 
TCCGGCGACC CTGTCAGTGT GGTGAAAGGA CTTATTCTGC TAATAGACCA TTCTCAGGAT 
ATTCAGCTGC AATCTGGACT AAAGGCCAAT ATGGACATCC AGGGTGGTCT AGCCATTGAC 
ATTTCTGGTT CAATGGAATT CAGTCTGTGG TACCGCGAGT CTAAAACCCG AGTGAAAAAT 
CGGGTGGCTG TGGTAATAAC CAGCGACATC ACAGTGGACT CGTCTTTTGT GAAAGCTGGT 
CTGGAAAGCA GAGCAGAGAC AGAGGCTGGC CTGGAGTTCA TCTCCACAGT GCAGTTCTCC 
CAGTACCCGT TCTTGGTCTG CATGCAGATG GACAAAGCCG AGGCTCCACT CAGGCAATTT 
GAGACAAAGT ATGAAAGGCT ATCCACAGGG AGGGGCTATG TCTCTCGGAG AAGAAAAGAG 
AGCCTAGTGC CCGGATGTGA ACTCCCGCTC CATCAAGAGA ACTCTGAGAT GTGCAACGTG 
GTATTCCCGC CTCAGCCAGA GAGCGGCAAC TCCGGTGGTG GATGGTTTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001101197.1
CDS	99..2789
Translation	MILLAVLFLCFFSSYSASVKGHTTGLSLNNERLYKLTYSTEVFLDGGKGKLQDSVGYRISSDVDVVLLWRNPDGDDDQLIQVTITAVNVENAGQQRGEKSIFKGKSTPKIVGKDNLEALRRPMLLHLVRGKVKEFYSYENEPVGIENLKRGLASLFQMQLTSGTTNEVDISGDCKVTYQAQQDKVVKIKALDTCKIERSGFTTANQVLGVTSKATSVTTYKIEDSFVTAVVAEETRAFALNFLQTVAGKIVSKQKLELKTTEAGPRMVPGKQVAGVIKAIDSKYKAIPIVGQVLQSVCKGCPSLAEHWQSIRKHLEPENLSNAEAVQSFLAFIQHLRTSRREEILQILKAEKKEVLPQLVDAVTSAQTPASLEAILDFLDFKSDSSIILQERFLYACGFASHPDEELLQALLSKFKGSFASNDIRESVMIIIGALVRKLCQNEGCKLKAVVEAKKLILGGLEKPEKKEDTTMYLLALKNALLPEGIPLLLKYAEAGEGPVSHLATTVLQRYDASFITDEVKKTLNRIYHQNRKVHEKTVRTTAAAVILKNNPSYMDVKNILLSIGELPKEMNKYMLTIVQDILHFEMPASKMIRRVLKEMIVHNYDRFSKSGSSSAYTGYVERSPHAASTYSLDILYSGSGILRRSNLNIFQYIGKAELHGSQVVIEAQGLEGLIAATPDEGEENLDSYAGMSAILFDVQLRPVTFFNGYSDLMSKMLSASGDPVSVVKGLILLIDHSQDIQLQSGLKANMDIQGGLAIDISGSMEFSLWYRESKTRVKNRVAVVITSDITVDSSFVKAGLESRAETEAGLEFISTVQFSQYPFLVCMQMDKAEAPLRQFETKYERLSTGRGYVSRRRKESLVPGCELPLHQENSEMCNVVFPPQPESGNSGGGWF

Target ORF information:

RefSeq Version	NM_001107727.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus microsomal triglyceride transfer protein (Mttp), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001107727.1

ORF Insert Sequence:

ATGATCCTCT TAGCTGTGCT TTTCCTCTGT TTCTTCTCCT CGTATTCTGC TTCTGTCAAA 
GGTCACACAA CTGGCCTCTC ATTAAATAAT GAGCGGCTAT ACAAGCTCAC GTACTCCACC 
GAAGTGTTTC TCGATGGGGG CAAAGGAAAA CTGCAGGACA GCGTGGGCTA CCGGATCTCA 
TCGGACGTGG ACGTTGTGTT ACTGTGGAGG AATCCCGACG GTGACGATGA TCAACTGATC 
CAAGTCACGA TAACGGCTGT CAATGTCGAA AATGCGGGTC AGCAGAGAGG AGAGAAGAGC 
ATCTTCAAGG GCAAAAGTAC ACCTAAGATC GTAGGAAAGG ACAACTTGGA GGCTCTACGG 
AGACCCATGC TTCTTCATCT GGTCCGGGGG AAGGTCAAAG AGTTCTACTC CTATGAAAAT 
GAGCCCGTGG GCATAGAAAA TCTCAAGAGA GGCTTGGCTA GCTTATTCCA GATGCAGTTA 
ACCTCTGGAA CCACCAATGA GGTAGATATC TCTGGGGATT GTAAAGTCAC CTACCAGGCT 
CAGCAAGACA AAGTGGTCAA AATTAAGGCT CTGGATACAT GCAAAATTGA GAGGTCCGGA 
TTTACAACAG CAAATCAGGT GCTGGGTGTC ACTTCAAAAG CCACCTCTGT CACTACCTAT 
AAGATAGAGG ACAGCTTTGT CACAGCAGTG GTTGCAGAAG AGACCAGGGC TTTTGCCTTG 
AACTTCCTAC AAACCGTGGC AGGAAAAATA GTGTCGAAGC AGAAATTGGA GCTGAAGACA 
ACGGAAGCCG GCCCAAGAAT GGTACCTGGG AAGCAAGTGG CAGGAGTAAT TAAGGCAATT 
GATTCCAAGT ACAAAGCCAT TCCCATTGTG GGACAGGTCC TCCAGAGTGT CTGTAAAGGC 
TGTCCTTCTC TGGCGGAGCA CTGGCAATCT ATCAGGAAGC ACCTAGAACC TGAGAACCTG 
TCCAACGCCG AGGCCGTCCA GAGCTTCCTG GCCTTCATTC AGCACCTCCG CACTTCGAGG 
AGAGAAGAGA TCCTTCAGAT TCTGAAGGCA GAGAAGAAAG AAGTTCTCCC TCAGCTGGTG 
GACGCCGTCA CCTCTGCTCA GACTCCAGCC TCACTGGAAG CCATCCTGGA CTTTTTGGAT 
TTCAAAAGCG ACAGCAGTAT CATTCTCCAG GAAAGGTTCC TCTATGCATG TGGATTTGCC 
TCCCATCCTG ATGAAGAACT CCTGCAAGCC CTCCTTAGCA AGTTCAAAGG TTCCTTTGCA 
AGCAACGACA TCAGAGAGTC GGTTATGATC ATCATTGGAG CCCTAGTCAG GAAGCTGTGT 
CAGAATGAAG GCTGCAAGCT CAAGGCAGTG GTTGAAGCTA AGAAGCTAAT CCTGGGAGGA 
CTTGAAAAAC CAGAGAAGAA AGAGGACACC ACGATGTACC TGCTGGCTCT GAAGAACGCC 
CTGCTTCCTG AAGGCATCCC GCTCCTTTTG AAGTACGCTG AGGCTGGGGA AGGGCCCGTC 
AGTCACCTGG CTACCACTGT TCTCCAGAGA TACGATGCCT CCTTCATCAC AGACGAGGTG 
AAGAAGACCT TGAACAGGAT ATACCATCAG AATCGTAAGG TTCATGAAAA GACTGTACGC 
ACAACGGCTG CTGCTGTCAT CTTAAAGAAC AACCCCTCCT ACATGGATGT GAAGAACATT 
CTGCTGTCTA TTGGGGAACT TCCTAAAGAA ATGAACAAAT ACATGCTCAC CATCGTGCAA 
GACATCCTGC ATTTCGAAAT GCCTGCAAGC AAAATGATCC GTCGGGTTCT CAAGGAGATG 
ATTGTTCACA ATTATGACCG TTTCTCCAAG AGTGGATCCT CTTCTGCCTA CACTGGCTAC 
GTAGAACGCA GTCCCCACGC GGCGTCCACC TACAGCCTTG ATATCCTCTA CTCTGGGTCT 
GGCATTCTGA GGAGAAGTAA CCTGAACATC TTCCAGTACA TCGGAAAGGC GGAGCTGCAT 
GGCAGCCAGG TGGTCATTGA AGCCCAAGGG CTGGAAGGCT TAATTGCAGC CACTCCAGAT 
GAGGGAGAGG AGAACCTTGA CTCGTATGCT GGTATGTCAG CCATCCTGTT TGATGTTCAG 
CTTAGACCTG TCACGTTCTT TAACGGATAC AGTGATTTGA TGTCCAAAAT GCTGTCGGCG 
TCCGGCGACC CTGTCAGTGT GGTGAAAGGA CTTATTCTGC TAATAGACCA TTCTCAGGAT 
ATTCAGCTGC AATCTGGACT AAAGGCCAAT ATGGACATCC AGGGTGGTCT AGCCATTGAC 
ATTTCTGGTT CAATGGAATT CAGTCTGTGG TACCGCGAGT CTAAAACCCG AGTGAAAAAT 
CGGGTGGCTG TGGTAATAAC CAGCGACATC ACAGTGGACT CGTCTTTTGT GAAAGCTGGT 
CTGGAAAGCA GAGCAGAGAC AGAGGCTGGC CTGGAGTTCA TCTCCACAGT GCAGTTCTCC 
CAGTACCCGT TCTTGGTCTG CATGCAGATG GACAAAGCCG AGGCTCCACT CAGGCAATTT 
GAGACAAAGT ATGAAAGGCT ATCCACAGGG AGGGGCTATG TCTCTCGGAG AAGAAAAGAG 
AGCCTAGTGC CCGGATGTGA ACTCCCGCTC CATCAAGAGA ACTCTGAGAT GTGCAACGTG 
GTATTCCCGC CTCAGCCAGA GAGCGGCAAC TCCGGTGGTG GATGGTTTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

?-Linolenic acid supplementation and exercise training reveal independent and additive responses on hepatic lipid accumulation in obese rats.
American journal of physiology. Endocrinology and metabolism312(6)E461-E470(2017 Jun)
Miotto PM,Horbatuk M,Proudfoot R,Matravadia S,Bakovic M,Chabowski A,Holloway GP

Novel Abetalipoproteinemia Missense Mutation Highlights the Importance of the N-Terminal ?-Barrel in Microsomal Triglyceride Transfer Protein Function.
Circulation. Cardiovascular genetics8(5)677-87(2015 Oct)
Walsh MT,Iqbal J,Josekutty J,Soh J,Di Leo E,?zaydin E,G?nd?z M,Tarugi P,Hussain MM

Improvement in the expression of hepatic genes involved in fatty acid metabolism in obese rats supplemented with taurine.
Life sciences13515-21(2015 Aug)
Bonfleur ML,Borck PC,Ribeiro RA,Caetano LC,Soares GM,Carneiro EM,Balbo SL

Up-Regulation Mttp and Apob Gene Expression in Rat Liver is Related to Post-Lipectomy Hypertriglyceridemia.
Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology36(5)1767-77(2015)
Dettlaff-Pokora A,Sledzinski T,Swierczynski J

Novel missense MTTP gene mutations causing abetalipoproteinemia.
Biochimica et biophysica acta1842(10)1548-54(2014 Oct)
Miller SA,Burnett JR,Leonis MA,McKnight CJ,van Bockxmeer FM,Hooper AJ

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Mttp cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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