Bcar3 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Bcar3
Entrez Gene ID	310838
Full Name	BCAR3, NSP family adaptor protein
General protein information	Preferred Names BCAR3, NSP family adaptor protein Names breast cancer anti-estrogen resistance protein 3 BCAR3, NSP family adaptor protein 3 breast cancer anti-estrogen resistance 3
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 2 Map Location: 2q42

mRNA and Protein(s)

mRNA	Protein	Name
XM_006233270.3	XP_006233332.1	breast cancer anti-estrogen resistance protein 3 isoform X1
NM_001107722.1	NP_001101192.1	breast cancer anti-estrogen resistance protein 3

Pathways from BioSystems

Homologies

Homo sapiens (human)	BCAR3	NP_001248337.1
Rattus norvegicus (Norway rat)	Bcar3	NP_001101192.1
Gallus gallus (chicken)	BCAR3	XP_422334.3
	LOC713636	XP_002801702.1
Xenopus tropicalis (tropical clawed frog)	bcar3	NP_001090734.1
Pan troglodytes (chimpanzee)	BCAR3	XP_001155709.1
Canis lupus familiaris (dog)	BCAR3	XP_853097.2
Bos taurus (cattle)	BCAR3	NP_001019654.1
Mus musculus (house mouse)	Bcar3	NP_038895.1
Danio rerio (zebrafish)	bcar3	NP_001107092.1

Gene Ontology
Bibliography

Related articles in PubMed

Functional roles of BCAR3 in the signaling pathways of insulin leading to DNA synthesis, membrane ruffling and GLUT4 translocation.
Oh MJ, Yi SJ, Kim HS, Kim JH, Jeong YH, van Agthoven T, Jhun BH
Biochemical and biophysical research communications441(4)911-6(2013 Nov)

Protein tyrosine phosphatase ? phosphotyrosyl-789 binds BCAR3 to position Cas for activation at integrin-mediated focal adhesions.
Sun G, Cheng SY, Chen M, Lim CJ, Pallen CJ
Molecular and cellular biology32(18)3776-89(2012 Sep)

Loss of AND-34/BCAR3 expression in mice results in rupture of the adult lens.
Near RI, Smith RS, Toselli PA, Freddo TF, Bloom AB, Vanden Borre P, Seldin DC, Lerner A
Molecular vision15685-99(2009)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

study described a molecular complex of PTPalpha-BCAR3-Cas-Src; this complex forms in response to PTPalpha Tyr789 phosphorylation and mediates Cas localization to focal adhesions and Cas downstream signaling to promote cell migration
Title: Protein tyrosine phosphatase ? phosphotyrosyl-789 binds BCAR3 to position Cas for activation at integrin-mediated focal adhesions.

The following Bcar3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Bcar3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa05748	NM_001107722.1 Latest version!	Rattus norvegicus BCAR3 adaptor protein, NSP family member (Bcar3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
ORa25238	XM_006233270.3 Latest version!	Rattus norvegicus breast cancer anti-estrogen resistance 3 (Bcar3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Bcar3 ( NM_001107722.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001101192.1 Rattus norvegicus BCAR3 adaptor protein, NSP family member (Bcar3), mRNA.

Price & Availability↑

CloneID	ORa05748
Clone ID Related Accession (Same CDS sequence)	NM_001107722.1
Accession Version	NM_001107722.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2478bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-28
Organism	Rattus norvegicus(Norway rat)
Product	breast cancer anti-estrogen resistance protein 3
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CH473952.1. On or before Oct 4, 2007 this sequence version replaced XM_001074184.1, XM_227657.4. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMD00052296, SAMEA2689594 [ECO:0000348] ##Evidence-Data-END##

ATGGCTGCGG GAAAATTTGC AAGCCTTCCC AGACACATGC CCGTGAATCA CCAGTTCCCT 
TTGGCCTCGT CCATGGACCT CCTGAGCAGC AAGTCCCCTC TCGCTGAGCA TCGCACAGAG 
GCCTATCCAG ACGTGTCCAT ACACGGCACT CTTCCACGGA AGAAAAAAGG CCCTCCTCCC 
ATACGGTCCT GTGACAGTGC CAGTCACATG GGCACCCTCC CTCACTCCAA GTCCCCACGA 
CAGAGCTCAC CTCTGACCCA GGACCTCATC CTGGAAAAAC CACTGCCAGA CTGGAAAGGG 
GACAGCTTCG CCTTCAGGGA TCCCTATCTT CTGGACCCAA CTCTGGAGTA CGTGAAGTTC 
TCCAAGGAGA GGCACATCAT GGACAGGACC CCTGAGAGGC TGAAAAAGGA ACTGGAGGAG 
GAGCTACTGC TGAGCAGCGA GGACCTACGC AGCCATGCCT GGTACCACGG CCGGATCCCC 
AGACAGGTGT CTGAAAACCT TGTGCAGCGG GATGGGGACT TCCTGGTTCG TGACTCCCTG 
TCTAGCCCTG GAAACTTTGT CCTGACCTGT CAGTGGAAGA ACCTTGCTCA GCACTTTAAG 
ATCAACCGGA CTGTTCTGCG GCTCAGCGAG GCCTACAGCC GTGTGCAGTA CCAATTCGAG 
ATGGAGAGCT TTGACTCCAT CCCCGGGCTG GTACGCTGCT ACGTGGGAAA CCGGAGGCCC 
ATCTCCCAGC AGAGCGGCGC CATCATCTTC CAGCCTATCA ATAGGACAGT GCCCCTTTGG 
TGTCTGGAGG AGCGTTATGG CACCTCACCA GGCCGAGGCC GGGAGGGCAG CTTTGCTGAG 
GGAAGACCAG ATGTGGTGAA GAGACTGAGC CTCACCACAG GTGGCATCCA GGCTCGGGAC 
CACAGCTTGC CCAGAGGAAA CCTCCTCAGG AATAAGGACA AGAGTGGCAG CCAGCCCGCC 
TGCCTGGATC ACGTGCAGGA CCGGAAGGCC GCAACACTCA AGGCTCACCA GTCTGAGAGT 
CACCTGCCCA TCGGCTGCAA GCTGCCACCC CAGTCTCCGA GTGTGGACAC AAGCCCTTGC 
CCCAACTCTC CCGTGTTCAG GACTGGCAGC GAGCCCACTC TGAGTCCGGC ACTGGTACGA 
AGGTTCTCTT CGGACGCTAG GGCAGGGGAG GCACTTCGGG GATCAGACAG TCAACTGTGC 
CCCAAGCCAC CCCCAAAGCC CTGCAAGGTG CCCTTCCTCA AGGTTCCTCC CTCTCCATCT 
CCCTGGCTAA ACTCAGAGGC CAACTACTGT GAACTGAACC CTGCTTTTGC GGTGGGCTGT 
GACAGGGGAG CCAAGCTTCT CTCACAGGCT CTCGACAGCC ACGAGATGCT GCTGACAGCC 
AAACAGAATG GGGCATCGGG TCCCCGGAAC TCTGGCATCA ACTACTCGAT ACTTGATGGG 
GATGACCAAG GGAGACATTG GGATCCCCTG GCCGTGCAGA CGGATGAGGG TCAGGAGGAC 
GAGACCAAGT TTGTACCACC TGTCATGGAG ACAGTGTCAT CATTCAGACC CAATGACTTT 
GAGTCGAAGC TTCTTCCTCC AGAGAACAAG CCCCTGGAAA CAGCCATGCT AAAGCATGCC 
AAGGAACTGT TCACCAACCA TGACGCGAGG GTCATTGCAC AGCACATGCT GAGCGTGGAC 
TGCAAGGTTG CTAGGATACT GGAAGTCTCT GAAGACATGA AGAGGAGCAT GGGCGTGAGC 
TCGGGACTGG AACTCATTAC CTTGCCTCAC GGACGCCAGC TGCGCCTGGA CATTATTGAA 
AGACACAACA CCATGGCCAT TGGCATTGCT GTGGACATTC TGGGCTGCAC GGGCACATTG 
GAGAACCGGG CAGGAACCCT TAATAAGATC ATCCAGGTGG CAATGGAACT GAAGGACACC 
ATGGGAGACC TCTATTCCTT CTCAGCCATC ATGAAAGCCC TCGAGATGCC TCAGATCACA 
AGGTTAGAAA AAACATGGAC GGCTCTGAGG CACCACTACA CGCAAACAGC CATCCTCTAC 
GAAAAGCAGT TGAAGCCCTT TAGCAAAATC CTGCATGAAG GCAGAGAGTC GACATATGTC 
CCAGCAAGCA GTGTGTCCGT CCCACTGCTG ATGCCTCTTG TGACCTTAAT GGAACGGCAG 
GCTGTCACTT TTGAAGGAAC CGACATGTGG GAAAAAAATG ACGAGAGCTG TGAAATCATG 
CTGAGTCACT TGGCCACAGC CAGGTTCATG GCCGAGGCTT CGGAGAGCTA CAGGATGAAT 
GCTGAGAGGG TCCTGGCAGA TTTCCAACCG GATGAAGAAA TGACTGAAAT CCTAAAGACT 
GAGTTCCAGA TGCGATTGTT GTGGGGCAGC AAGGGTGCTG AAGTCAACCA GAACGAGAGA 
TATGACAAGT TCAACCAGAT CCTAACTGCC CTCTCACGGA AACTGGAACC TCCTTCTGGA 
AAGCAGGCTG AGCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001101192.1
CDS	425..2902
Translation	MAAGKFASLPRHMPVNHQFPLASSMDLLSSKSPLAEHRTEAYPDVSIHGTLPRKKKGPPPIRSCDSASHMGTLPHSKSPRQSSPLTQDLILEKPLPDWKGDSFAFRDPYLLDPTLEYVKFSKERHIMDRTPERLKKELEEELLLSSEDLRSHAWYHGRIPRQVSENLVQRDGDFLVRDSLSSPGNFVLTCQWKNLAQHFKINRTVLRLSEAYSRVQYQFEMESFDSIPGLVRCYVGNRRPISQQSGAIIFQPINRTVPLWCLEERYGTSPGRGREGSFAEGRPDVVKRLSLTTGGIQARDHSLPRGNLLRNKDKSGSQPACLDHVQDRKAATLKAHQSESHLPIGCKLPPQSPSVDTSPCPNSPVFRTGSEPTLSPALVRRFSSDARAGEALRGSDSQLCPKPPPKPCKVPFLKVPPSPSPWLNSEANYCELNPAFAVGCDRGAKLLSQALDSHEMLLTAKQNGASGPRNSGINYSILDGDDQGRHWDPLAVQTDEGQEDETKFVPPVMETVSSFRPNDFESKLLPPENKPLETAMLKHAKELFTNHDARVIAQHMLSVDCKVARILEVSEDMKRSMGVSSGLELITLPHGRQLRLDIIERHNTMAIGIAVDILGCTGTLENRAGTLNKIIQVAMELKDTMGDLYSFSAIMKALEMPQITRLEKTWTALRHHYTQTAILYEKQLKPFSKILHEGRESTYVPASSVSVPLLMPLVTLMERQAVTFEGTDMWEKNDESCEIMLSHLATARFMAEASESYRMNAERVLADFQPDEEMTEILKTEFQMRLLWGSKGAEVNQNERYDKFNQILTALSRKLEPPSGKQAEL

Target ORF information:

RefSeq Version	NM_001107722.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus BCAR3 adaptor protein, NSP family member (Bcar3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001107722.1

ORF Insert Sequence:

ATGGCTGCGG GAAAATTTGC AAGCCTTCCC AGACACATGC CCGTGAATCA CCAGTTCCCT 
TTGGCCTCGT CCATGGACCT CCTGAGCAGC AAGTCCCCTC TCGCTGAGCA TCGCACAGAG 
GCCTATCCAG ACGTGTCCAT ACACGGCACT CTTCCACGGA AGAAAAAAGG CCCTCCTCCC 
ATACGGTCCT GTGACAGTGC CAGTCACATG GGCACCCTCC CTCACTCCAA GTCCCCACGA 
CAGAGCTCAC CTCTGACCCA GGACCTCATC CTGGAAAAAC CACTGCCAGA CTGGAAAGGG 
GACAGCTTCG CCTTCAGGGA TCCCTATCTT CTGGACCCAA CTCTGGAGTA CGTGAAGTTC 
TCCAAGGAGA GGCACATCAT GGACAGGACC CCTGAGAGGC TGAAAAAGGA ACTGGAGGAG 
GAGCTACTGC TGAGCAGCGA GGACCTACGC AGCCATGCCT GGTACCACGG CCGGATCCCC 
AGACAGGTGT CTGAAAACCT TGTGCAGCGG GATGGGGACT TCCTGGTTCG TGACTCCCTG 
TCTAGCCCTG GAAACTTTGT CCTGACCTGT CAGTGGAAGA ACCTTGCTCA GCACTTTAAG 
ATCAACCGGA CTGTTCTGCG GCTCAGCGAG GCCTACAGCC GTGTGCAGTA CCAATTCGAG 
ATGGAGAGCT TTGACTCCAT CCCCGGGCTG GTACGCTGCT ACGTGGGAAA CCGGAGGCCC 
ATCTCCCAGC AGAGCGGCGC CATCATCTTC CAGCCTATCA ATAGGACAGT GCCCCTTTGG 
TGTCTGGAGG AGCGTTATGG CACCTCACCA GGCCGAGGCC GGGAGGGCAG CTTTGCTGAG 
GGAAGACCAG ATGTGGTGAA GAGACTGAGC CTCACCACAG GTGGCATCCA GGCTCGGGAC 
CACAGCTTGC CCAGAGGAAA CCTCCTCAGG AATAAGGACA AGAGTGGCAG CCAGCCCGCC 
TGCCTGGATC ACGTGCAGGA CCGGAAGGCC GCAACACTCA AGGCTCACCA GTCTGAGAGT 
CACCTGCCCA TCGGCTGCAA GCTGCCACCC CAGTCTCCGA GTGTGGACAC AAGCCCTTGC 
CCCAACTCTC CCGTGTTCAG GACTGGCAGC GAGCCCACTC TGAGTCCGGC ACTGGTACGA 
AGGTTCTCTT CGGACGCTAG GGCAGGGGAG GCACTTCGGG GATCAGACAG TCAACTGTGC 
CCCAAGCCAC CCCCAAAGCC CTGCAAGGTG CCCTTCCTCA AGGTTCCTCC CTCTCCATCT 
CCCTGGCTAA ACTCAGAGGC CAACTACTGT GAACTGAACC CTGCTTTTGC GGTGGGCTGT 
GACAGGGGAG CCAAGCTTCT CTCACAGGCT CTCGACAGCC ACGAGATGCT GCTGACAGCC 
AAACAGAATG GGGCATCGGG TCCCCGGAAC TCTGGCATCA ACTACTCGAT ACTTGATGGG 
GATGACCAAG GGAGACATTG GGATCCCCTG GCCGTGCAGA CGGATGAGGG TCAGGAGGAC 
GAGACCAAGT TTGTACCACC TGTCATGGAG ACAGTGTCAT CATTCAGACC CAATGACTTT 
GAGTCGAAGC TTCTTCCTCC AGAGAACAAG CCCCTGGAAA CAGCCATGCT AAAGCATGCC 
AAGGAACTGT TCACCAACCA TGACGCGAGG GTCATTGCAC AGCACATGCT GAGCGTGGAC 
TGCAAGGTTG CTAGGATACT GGAAGTCTCT GAAGACATGA AGAGGAGCAT GGGCGTGAGC 
TCGGGACTGG AACTCATTAC CTTGCCTCAC GGACGCCAGC TGCGCCTGGA CATTATTGAA 
AGACACAACA CCATGGCCAT TGGCATTGCT GTGGACATTC TGGGCTGCAC GGGCACATTG 
GAGAACCGGG CAGGAACCCT TAATAAGATC ATCCAGGTGG CAATGGAACT GAAGGACACC 
ATGGGAGACC TCTATTCCTT CTCAGCCATC ATGAAAGCCC TCGAGATGCC TCAGATCACA 
AGGTTAGAAA AAACATGGAC GGCTCTGAGG CACCACTACA CGCAAACAGC CATCCTCTAC 
GAAAAGCAGT TGAAGCCCTT TAGCAAAATC CTGCATGAAG GCAGAGAGTC GACATATGTC 
CCAGCAAGCA GTGTGTCCGT CCCACTGCTG ATGCCTCTTG TGACCTTAAT GGAACGGCAG 
GCTGTCACTT TTGAAGGAAC CGACATGTGG GAAAAAAATG ACGAGAGCTG TGAAATCATG 
CTGAGTCACT TGGCCACAGC CAGGTTCATG GCCGAGGCTT CGGAGAGCTA CAGGATGAAT 
GCTGAGAGGG TCCTGGCAGA TTTCCAACCG GATGAAGAAA TGACTGAAAT CCTAAAGACT 
GAGTTCCAGA TGCGATTGTT GTGGGGCAGC AAGGGTGCTG AAGTCAACCA GAACGAGAGA 
TATGACAAGT TCAACCAGAT CCTAACTGCC CTCTCACGGA AACTGGAACC TCCTTCTGGA 
AAGCAGGCTG AGCTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Bcar3 ( XM_006233270.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006233332.1 Rattus norvegicus breast cancer anti-estrogen resistance 3 (Bcar3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa25238
Clone ID Related Accession (Same CDS sequence)	XM_006233270.3
Accession Version	XM_006233270.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2205bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	breast cancer anti-estrogen resistance protein 3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005101.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006233270.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCTAAGG AATGCAGTGC CTTCCATGCC CTCTCCGCAG CCCTGTGCTG TTTCTATCAG 
CGCAAGTCTT TCTTTGGAGT CAAGTTCTCC AAGGAGAGGC ACATCATGGA CAGGACCCCT 
GAGAGGCTGA AAAAGGAACT GGAGGAGGAG CTACTGCTGA GCAGCGAGGA CCTACGCAGC 
CATGCCTGGT ACCACGGCCG GATCCCCAGA CAGGTGTCTG AAAACCTTGT GCAGCGGGAT 
GGGGACTTCC TGGTTCGTGA CTCCCTGTCT AGCCCTGGAA ACTTTGTCCT GACCTGTCAG 
TGGAAGAACC TTGCTCAGCA CTTTAAGATC AACCGGACTG TTCTGCGGCT CAGCGAGGCC 
TACAGCCGTG TGCAGTACCA ATTCGAGATG GAGAGCTTTG ACTCCATCCC CGGGCTGGTA 
CGCTGCTACG TGGGAAACCG GAGGCCCATC TCCCAGCAGA GCGGCGCCAT CATCTTCCAG 
CCTATCAATA GGACAGTGCC CCTTTGGTGT CTGGAGGAGC GTTATGGCAC CTCACCAGGC 
CGAGGCCGGG AGGGCAGCTT TGCTGAGGGA AGACCAGATG TGGTGAAGAG ACTGAGCCTC 
ACCACAGGTG GCATCCAGGC TCGGGACCAC AGCTTGCCCA GAGGAAACCT CCTCAGGAAT 
AAGGACAAGA GTGGCAGCCA GCCCGCCTGC CTGGATCACG TGCAGGACCG GAAGGCCGCA 
ACACTCAAGG CTCACCAGTC TGAGAGTCAC CTGCCCATCG GCTGCAAGCT GCCACCCCAG 
TCTCCGAGTG TGGACACAAG CCCTTGCCCC AACTCTCCCG TGTTCAGGAC TGGCAGCGAG 
CCCACTCTGA GTCCGGCACT GGTACGAAGG TTCTCTTCGG ACGCTAGGGC AGGGGAGGCA 
CTTCGGGGAT CAGACAGTCA ACTGTGCCCC AAGCCACCCC CAAAGCCCTG CAAGGTGCCC 
TTCCTCAAGG TTCCTCCCTC TCCATCTCCC TGGCTAAACT CAGAGGCCAA CTACTGTGAA 
CTGAACCCTG CTTTTGCGGT GGGCTGTGAC AGGGGAGCCA AGCTTCTCTC ACAGGCTCTC 
GACAGCCACG AGATGCTGCT GACAGCCAAA CAGAATGGGG CATCGGGTCC CCGGAACTCT 
GGCATCAACT ACTCGATACT TGATGGGGAT GACCAAGGGA GACATTGGGA TCCCCTGGCC 
GTGCAGACGG ATGAGGGTCA GGAGGACGAG ACCAAGTTTG TACCACCTGT CATGGAGACA 
GTGTCATCAT TCAGACCCAA TGACTTTGAG TCGAAGCTTC TTCCTCCAGA GAACAAGCCC 
CTGGAAACAG CCATGCTAAA GCATGCCAAG GAACTGTTCA CCAACCATGA CGCGAGGGTC 
ATTGCACAGC ACATGCTGAG CGTGGACTGC AAGGTTGCTA GGATACTGGA AGTCTCTGAA 
GACATGAAGA GGAGCATGGG CGTGAGCTCG GGACTGGAAC TCATTACCTT GCCTCACGGA 
CGCCAGCTGC GCCTGGACAT TATTGAAAGA CACAACACCA TGGCCATTGG CATTGCTGTG 
GACATTCTGG GCTGCACGGG CACATTGGAG AACCGGGCAG GAACCCTTAA TAAGATCATC 
CAGGTGGCAA TGGAACTGAA GGACACCATG GGAGACCTCT ATTCCTTCTC AGCCATCATG 
AAAGCCCTCG AGATGCCTCA GATCACAAGG TTAGAAAAAA CATGGACGGC TCTGAGGCAC 
CACTACACGC AAACAGCCAT CCTCTACGAA AAGCAGTTGA AGCCCTTTAG CAAAATCCTG 
CATGAAGGCA GAGAGTCGAC ATATGTCCCA GCAAGCAGTG TGTCCGTCCC ACTGCTGATG 
CCTCTTGTGA CCTTAATGGA ACGGCAGGCT GTCACTTTTG AAGGAACCGA CATGTGGGAA 
AAAAATGACG AGAGCTGTGA AATCATGCTG AGTCACTTGG CCACAGCCAG GTTCATGGCC 
GAGGCTTCGG AGAGCTACAG GATGAATGCT GAGAGGGTCC TGGCAGATTT CCAACCGGAT 
GAAGAAATGA CTGAAATCCT AAAGACTGAG TTCCAGATGC GATTGTTGTG GGGCAGCAAG 
GGTGCTGAAG TCAACCAGAA CGAGAGATAT GACAAGTTCA ACCAGATCCT AACTGCCCTC 
TCACGGAAAC TGGAACCTCC TTCTGGAAAG CAGGCTGAGC TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006233332.1
CDS	2324..4528
Translation	MPKECSAFHALSAALCCFYQRKSFFGVKFSKERHIMDRTPERLKKELEEELLLSSEDLRSHAWYHGRIPRQVSENLVQRDGDFLVRDSLSSPGNFVLTCQWKNLAQHFKINRTVLRLSEAYSRVQYQFEMESFDSIPGLVRCYVGNRRPISQQSGAIIFQPINRTVPLWCLEERYGTSPGRGREGSFAEGRPDVVKRLSLTTGGIQARDHSLPRGNLLRNKDKSGSQPACLDHVQDRKAATLKAHQSESHLPIGCKLPPQSPSVDTSPCPNSPVFRTGSEPTLSPALVRRFSSDARAGEALRGSDSQLCPKPPPKPCKVPFLKVPPSPSPWLNSEANYCELNPAFAVGCDRGAKLLSQALDSHEMLLTAKQNGASGPRNSGINYSILDGDDQGRHWDPLAVQTDEGQEDETKFVPPVMETVSSFRPNDFESKLLPPENKPLETAMLKHAKELFTNHDARVIAQHMLSVDCKVARILEVSEDMKRSMGVSSGLELITLPHGRQLRLDIIERHNTMAIGIAVDILGCTGTLENRAGTLNKIIQVAMELKDTMGDLYSFSAIMKALEMPQITRLEKTWTALRHHYTQTAILYEKQLKPFSKILHEGRESTYVPASSVSVPLLMPLVTLMERQAVTFEGTDMWEKNDESCEIMLSHLATARFMAEASESYRMNAERVLADFQPDEEMTEILKTEFQMRLLWGSKGAEVNQNERYDKFNQILTALSRKLEPPSGKQAEL

Target ORF information:

RefSeq Version	XM_006233270.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus breast cancer anti-estrogen resistance 3 (Bcar3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006233270.3

ORF Insert Sequence:

ATGCCTAAGG AATGCAGTGC CTTCCATGCC CTCTCCGCAG CCCTGTGCTG TTTCTATCAG 
CGCAAGTCTT TCTTTGGAGT CAAGTTCTCC AAGGAGAGGC ACATCATGGA CAGGACCCCT 
GAGAGGCTGA AAAAGGAACT GGAGGAGGAG CTACTGCTGA GCAGCGAGGA CCTACGCAGC 
CATGCCTGGT ACCACGGCCG GATCCCCAGA CAGGTGTCTG AAAACCTTGT GCAGCGGGAT 
GGGGACTTCC TGGTTCGTGA CTCCCTGTCT AGCCCTGGAA ACTTTGTCCT GACCTGTCAG 
TGGAAGAACC TTGCTCAGCA CTTTAAGATC AACCGGACTG TTCTGCGGCT CAGCGAGGCC 
TACAGCCGTG TGCAGTACCA ATTCGAGATG GAGAGCTTTG ACTCCATCCC CGGGCTGGTA 
CGCTGCTACG TGGGAAACCG GAGGCCCATC TCCCAGCAGA GCGGCGCCAT CATCTTCCAG 
CCTATCAATA GGACAGTGCC CCTTTGGTGT CTGGAGGAGC GTTATGGCAC CTCACCAGGC 
CGAGGCCGGG AGGGCAGCTT TGCTGAGGGA AGACCAGATG TGGTGAAGAG ACTGAGCCTC 
ACCACAGGTG GCATCCAGGC TCGGGACCAC AGCTTGCCCA GAGGAAACCT CCTCAGGAAT 
AAGGACAAGA GTGGCAGCCA GCCCGCCTGC CTGGATCACG TGCAGGACCG GAAGGCCGCA 
ACACTCAAGG CTCACCAGTC TGAGAGTCAC CTGCCCATCG GCTGCAAGCT GCCACCCCAG 
TCTCCGAGTG TGGACACAAG CCCTTGCCCC AACTCTCCCG TGTTCAGGAC TGGCAGCGAG 
CCCACTCTGA GTCCGGCACT GGTACGAAGG TTCTCTTCGG ACGCTAGGGC AGGGGAGGCA 
CTTCGGGGAT CAGACAGTCA ACTGTGCCCC AAGCCACCCC CAAAGCCCTG CAAGGTGCCC 
TTCCTCAAGG TTCCTCCCTC TCCATCTCCC TGGCTAAACT CAGAGGCCAA CTACTGTGAA 
CTGAACCCTG CTTTTGCGGT GGGCTGTGAC AGGGGAGCCA AGCTTCTCTC ACAGGCTCTC 
GACAGCCACG AGATGCTGCT GACAGCCAAA CAGAATGGGG CATCGGGTCC CCGGAACTCT 
GGCATCAACT ACTCGATACT TGATGGGGAT GACCAAGGGA GACATTGGGA TCCCCTGGCC 
GTGCAGACGG ATGAGGGTCA GGAGGACGAG ACCAAGTTTG TACCACCTGT CATGGAGACA 
GTGTCATCAT TCAGACCCAA TGACTTTGAG TCGAAGCTTC TTCCTCCAGA GAACAAGCCC 
CTGGAAACAG CCATGCTAAA GCATGCCAAG GAACTGTTCA CCAACCATGA CGCGAGGGTC 
ATTGCACAGC ACATGCTGAG CGTGGACTGC AAGGTTGCTA GGATACTGGA AGTCTCTGAA 
GACATGAAGA GGAGCATGGG CGTGAGCTCG GGACTGGAAC TCATTACCTT GCCTCACGGA 
CGCCAGCTGC GCCTGGACAT TATTGAAAGA CACAACACCA TGGCCATTGG CATTGCTGTG 
GACATTCTGG GCTGCACGGG CACATTGGAG AACCGGGCAG GAACCCTTAA TAAGATCATC 
CAGGTGGCAA TGGAACTGAA GGACACCATG GGAGACCTCT ATTCCTTCTC AGCCATCATG 
AAAGCCCTCG AGATGCCTCA GATCACAAGG TTAGAAAAAA CATGGACGGC TCTGAGGCAC 
CACTACACGC AAACAGCCAT CCTCTACGAA AAGCAGTTGA AGCCCTTTAG CAAAATCCTG 
CATGAAGGCA GAGAGTCGAC ATATGTCCCA GCAAGCAGTG TGTCCGTCCC ACTGCTGATG 
CCTCTTGTGA CCTTAATGGA ACGGCAGGCT GTCACTTTTG AAGGAACCGA CATGTGGGAA 
AAAAATGACG AGAGCTGTGA AATCATGCTG AGTCACTTGG CCACAGCCAG GTTCATGGCC 
GAGGCTTCGG AGAGCTACAG GATGAATGCT GAGAGGGTCC TGGCAGATTT CCAACCGGAT 
GAAGAAATGA CTGAAATCCT AAAGACTGAG TTCCAGATGC GATTGTTGTG GGGCAGCAAG 
GGTGCTGAAG TCAACCAGAA CGAGAGATAT GACAAGTTCA ACCAGATCCT AACTGCCCTC 
TCACGGAAAC TGGAACCTCC TTCTGGAAAG CAGGCTGAGC TGTGA