Sema4a cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Sema4a
Entrez Gene ID	310630
Full Name	semaphorin 4A
General protein information	Preferred Names semaphorin 4A Names semaphorin-4A sema domain, immunoglobulin domain (Ig), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 4A
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 2 Map Location: 2q34

mRNA and Protein(s)

mRNA	Protein	Name
XM_006232640.3	XP_006232702.1	semaphorin-4A isoform X1
NM_001012078.2	NP_001012078.2	semaphorin-4A precursor
XM_006232641.3	XP_006232703.1	semaphorin-4A isoform X1
XM_006232639.3	XP_006232701.1	semaphorin-4A isoform X1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	SEMA4A	XP_005622764.1
Homo sapiens (human)	SEMA4A	NP_001180229.1
Pan troglodytes (chimpanzee)	SEMA4A	XP_001164043.1
	SEMA4A	XP_001116428.1
Bos taurus (cattle)	SEMA4A	NP_001068908.1
Mus musculus (house mouse)	Sema4a	NP_038686.3
Rattus norvegicus (Norway rat)	Sema4a	NP_001012078.2
Xenopus tropicalis (tropical clawed frog)	sema4a	NP_001107311.1

Gene Ontology
Bibliography

Related articles in PubMed

Sema4A induces cell morphological changes through B-type plexin-mediated signaling.
Yukawa K, Tanaka T, Yoshida K, Takeuchi N, Ito T, Takamatsu H, Kikutani H, Kumanogoh A
International journal of molecular medicine25(2)225-30(2010 Feb)

Semaphorin-4A, an activator for T-cell-mediated immunity, suppresses angiogenesis via Plexin-D1.
Toyofuku T, Yabuki M, Kamei J, Kamei M, Makino N, Kumanogoh A, Hori M
The EMBO journal26(5)1373-84(2007 Mar)

Nonredundant roles of Sema4A in the immune system: defective T cell priming and Th1/Th2 regulation in Sema4A-deficient mice.
Kumanogoh A, Shikina T, Suzuki K, Uematsu S, Yukawa K, Kashiwamura S, Tsutsui H, Yamamoto M, Takamatsu H, Ko-Mitamura EP, Takegahara N, Marukawa S, Ishida I, Morishita H, Prasad DV, Tamura M, Mizui M, Toyofuku T, Akira S, Takeda K, Okabe M, Kikutani H
Immunity22(3)305-16(2005 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Sema4a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Sema4a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa02196	NM_001012078.2 Latest version!	Rattus norvegicus semaphorin 4A (Sema4a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$391.30 $559.00
ORa02196	XM_006232641.3 Latest version!	Rattus norvegicus semaphorin 4A (Sema4a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$391.30 $559.00
ORa02196	XM_006232639.3 Latest version!	Rattus norvegicus semaphorin 4A (Sema4a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$391.30 $559.00
ORa02196	XM_006232640.3 Latest version!	Rattus norvegicus semaphorin 4A (Sema4a), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$391.30 $559.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Sema4a ( NM_001012078.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001012078.2 Rattus norvegicus semaphorin 4A (Sema4a), mRNA.

Price & Availability↑

CloneID	ORa02196
Clone ID Related Accession (Same CDS sequence)	XM_006232640.3 , NM_001012078.2 , XM_006232641.3 , XM_006232639.3
Accession Version	NM_001012078.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2289bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-18
Organism	Rattus norvegicus(Norway rat)
Product	semaphorin-4A precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC119762.7 and BC081943.1. On Sep 6, 2012 this sequence version replaced NM_001012078.1. ##Evidence-Data-START## Transcript exon combination :: BC081943.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2689599 [ECO:0000348] ##Evidence-Data-END##

ATGACCATGG CCCTACCATC CCTGGGCCAA GACCCCTGGA GTCTCCTGAG TGTTTTTTTC 
TTCCAACTGT TCCTGCTGCT GTCACTGCCA CCAGCTTCCG GGACTGGTGG GCAGGGACCC 
GTGCCCAGAG TCAAGTACCA TGCGGGAGAT GGGCACAGGG CCCTCAGCTT CTTTCAACAA 
AAAGGCCTCC GGGACTTTGA CACCCTGCTC CTGAGTGGAG ATGGCAACAC TCTGTATGTG 
GGGGCTCGGG AGGCCATCTT GGCCCTGAAT ATCGAGAATC CAGGAATCCC AAGGCTGAGG 
AACATGATAC CCTGGCCAGC CAGTGAGAAA AAAAAGAACG AATGTGCCTT TAAGAAGAAA 
AGCAATGAGA CACAGTGTTT CAACTTCATT CGTGTGCTGG TCTCTTACAA TGCCACTCAC 
CTCTATGCCT GCGGGACTTT TGCCTTCAGC CCCGCCTGCA CCTTCATTGA ACTCCAAGAT 
TCCTTCCTGT TGCCCATCTT GAAGGACAAG GTCACAGATG GGAAGGGTCA AAGCCCCTTT 
GACCCTGCTC ACAAGCACAC AGCTGTCTTG GTCGATGGGA TGCTTTATTC TGGCACCATG 
AACAACTTCC TGGGCAGCGA ACCCATCCTG ATGCGGACAC TGGGATCCCA GCCTGTTCTC 
AAGACTGACA TCTTCCTACG CTGGCTGCAC CCGGACGCCT CCTTCGTGGC AGCCATCCCA 
TCTACCCAGG TCGTCTATTT CTTCTTTGAG GAGACAGCCA GCGAGTTTGA CTTCTTTGAG 
GAGCTGTACA CATCCAGGGT GGCTCAAGTC TGCAAGAACG ACGTAGGCGG TGAAAAGCTG 
CTGCAGAAGA AGTGGACCAC CTTCCTGAAA GCCCAGCTGC TCTGCGCTCA GCCAGGGCAG 
CTGCCATTCA ACATCATCCG CCACGCGGTC CTGCTGCCCG CCGATTCTCC CTCTGTTCCC 
CGCATCTACG CAGTCTTTAC CTCCCAGTGG CAGGTTGGTG GGACCAGGAG CTCAGCAGTC 
TGTGCCTTCT CTCTCACGGA CATTGAGCGA GTCTTTAAAG GAAAGTACAA GGAGCTGAAC 
AAAGAAACCT CCCGCTGGAC CACTTACAGG GGCTCAGAGG TCAACCCGAG GCCAGGCAGC 
TGCTCCATGG GCCCCTCCTC TGACAAAGCC TTGACCTTCA TGAAGGACCA TTTTCTGATG 
GATGAGCATG TGGTAGGAGC TCCCCTGCTG GTGAAGTCTG GCGTGGAGTA CACACAGCTT 
GCCGTGGAAA CAGCTCAGGG CCTTGACGGG AGCAGCCATG TGGTCCTTTA CCTGGGTACC 
TCTACGGGGT CCCTGCACAA AGCTGTGGTG CCTCAGAATA GCAGTGCTTA TCTTGTGGAG 
GAGATTCAGC TGAGCCCTAA CTCTGAGCCT GTTCAGAACC TGCAGCTGGC CCCCACCCAG 
GGTGCAGTGT TCGCAGGCTT CTCTGAAGGC ATCTGGAGGG TTCCCAGGGC CAACTGCAGT 
GTCTATAAGA GCTGTGTGGA CTGTGTCCTT GCCAGGGACC CTCACTGTGC CTGGGACCCT 
GAATCAAGAC TGTGCAGCCT TCTGTCTGGC TCTCCCCTGA CTTGGAAGCA GGACATGGAA 
CGCGGCAACC CGGAGTGGGC ATGCGCCCGT GGCCCCATGG CCAGGAGCCC CCGGCGTCAG 
AGCTCCCCTC AACTCATTAA AGAAGTCCTG ACAGTCCCCA ACTCCATCCT GGAGCTGCCC 
TGCCCCCAGC TGTCAGCACT GGCCTCTTAC CACTGGAGTC ACGGCAAAGC CAAAATCCCA 
GAAGCCTCTT CTACTGTCTA TAATGGCTCC CTCTTGTTGC TGCCACAGGA TGGCGTTGGG 
GGCCTCTACC AGTGCGTGGC CACTGAAAAC GGCTATTCGT ACCCTGTAGT CTCCTACTGG 
GTAGACAGCC AGGACCAGCC CCTGGCACTG GATCCTGAGC TGGCGGGCGT TCCCCGGGAG 
CGAGTGCAGG TCCCACTGAC CAGGGTCGGA GGCGGGGCTT CTATGGCTGC CCAGCGGTCC 
TACTGGCCCC ATTTTCTCAT CGTTACTGTC CTCCTGGCCA TTGTGCTCTT GGGAGTGCTC 
ACTCTCCTCC TTGCTTCCCC ACTGGGAGTG CTTCGGGCTC GGGGTAAGGT TCAGGGCTGT 
GGGATGCTGC CCCCCAGGGA AAAGGCCCCA CTGAGCAGGG AACAGCACCT CCAGCCTTCC 
AAGGACCACA GGACCTCTGC CAGCGACGTA GATGCTGACA ACAACCATCT GGGCGCTGAA 
GTGGCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001012078.2
CDS	222..2510
Translation	MTMALPSLGQDPWSLLSVFFFQLFLLLSLPPASGTGGQGPVPRVKYHAGDGHRALSFFQQKGLRDFDTLLLSGDGNTLYVGAREAILALNIENPGIPRLRNMIPWPASEKKKNECAFKKKSNETQCFNFIRVLVSYNATHLYACGTFAFSPACTFIELQDSFLLPILKDKVTDGKGQSPFDPAHKHTAVLVDGMLYSGTMNNFLGSEPILMRTLGSQPVLKTDIFLRWLHPDASFVAAIPSTQVVYFFFEETASEFDFFEELYTSRVAQVCKNDVGGEKLLQKKWTTFLKAQLLCAQPGQLPFNIIRHAVLLPADSPSVPRIYAVFTSQWQVGGTRSSAVCAFSLTDIERVFKGKYKELNKETSRWTTYRGSEVNPRPGSCSMGPSSDKALTFMKDHFLMDEHVVGAPLLVKSGVEYTQLAVETAQGLDGSSHVVLYLGTSTGSLHKAVVPQNSSAYLVEEIQLSPNSEPVQNLQLAPTQGAVFAGFSEGIWRVPRANCSVYKSCVDCVLARDPHCAWDPESRLCSLLSGSPLTWKQDMERGNPEWACARGPMARSPRRQSSPQLIKEVLTVPNSILELPCPQLSALASYHWSHGKAKIPEASSTVYNGSLLLLPQDGVGGLYQCVATENGYSYPVVSYWVDSQDQPLALDPELAGVPRERVQVPLTRVGGGASMAAQRSYWPHFLIVTVLLAIVLLGVLTLLLASPLGVLRARGKVQGCGMLPPREKAPLSREQHLQPSKDHRTSASDVDADNNHLGAEVA

Target ORF information:

RefSeq Version	NM_001012078.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus semaphorin 4A (Sema4a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001012078.2

ORF Insert Sequence:

ATGACCATGG CCCTACCATC CCTGGGCCAA GACCCCTGGA GTCTCCTGAG TGTTTTTTTC 
TTCCAACTGT TCCTGCTGCT GTCACTGCCA CCAGCTTCCG GGACTGGTGG GCAGGGACCC 
GTGCCCAGAG TCAAGTACCA TGCGGGAGAT GGGCACAGGG CCCTCAGCTT CTTTCAACAA 
AAAGGCCTCC GGGACTTTGA CACCCTGCTC CTGAGTGGAG ATGGCAACAC TCTGTATGTG 
GGGGCTCGGG AGGCCATCTT GGCCCTGAAT ATCGAGAATC CAGGAATCCC AAGGCTGAGG 
AACATGATAC CCTGGCCAGC CAGTGAGAAA AAAAAGAACG AATGTGCCTT TAAGAAGAAA 
AGCAATGAGA CACAGTGTTT CAACTTCATT CGTGTGCTGG TCTCTTACAA TGCCACTCAC 
CTCTATGCCT GCGGGACTTT TGCCTTCAGC CCCGCCTGCA CCTTCATTGA ACTCCAAGAT 
TCCTTCCTGT TGCCCATCTT GAAGGACAAG GTCACAGATG GGAAGGGTCA AAGCCCCTTT 
GACCCTGCTC ACAAGCACAC AGCTGTCTTG GTCGATGGGA TGCTTTATTC TGGCACCATG 
AACAACTTCC TGGGCAGCGA ACCCATCCTG ATGCGGACAC TGGGATCCCA GCCTGTTCTC 
AAGACTGACA TCTTCCTACG CTGGCTGCAC CCGGACGCCT CCTTCGTGGC AGCCATCCCA 
TCTACCCAGG TCGTCTATTT CTTCTTTGAG GAGACAGCCA GCGAGTTTGA CTTCTTTGAG 
GAGCTGTACA CATCCAGGGT GGCTCAAGTC TGCAAGAACG ACGTAGGCGG TGAAAAGCTG 
CTGCAGAAGA AGTGGACCAC CTTCCTGAAA GCCCAGCTGC TCTGCGCTCA GCCAGGGCAG 
CTGCCATTCA ACATCATCCG CCACGCGGTC CTGCTGCCCG CCGATTCTCC CTCTGTTCCC 
CGCATCTACG CAGTCTTTAC CTCCCAGTGG CAGGTTGGTG GGACCAGGAG CTCAGCAGTC 
TGTGCCTTCT CTCTCACGGA CATTGAGCGA GTCTTTAAAG GAAAGTACAA GGAGCTGAAC 
AAAGAAACCT CCCGCTGGAC CACTTACAGG GGCTCAGAGG TCAACCCGAG GCCAGGCAGC 
TGCTCCATGG GCCCCTCCTC TGACAAAGCC TTGACCTTCA TGAAGGACCA TTTTCTGATG 
GATGAGCATG TGGTAGGAGC TCCCCTGCTG GTGAAGTCTG GCGTGGAGTA CACACAGCTT 
GCCGTGGAAA CAGCTCAGGG CCTTGACGGG AGCAGCCATG TGGTCCTTTA CCTGGGTACC 
TCTACGGGGT CCCTGCACAA AGCTGTGGTG CCTCAGAATA GCAGTGCTTA TCTTGTGGAG 
GAGATTCAGC TGAGCCCTAA CTCTGAGCCT GTTCAGAACC TGCAGCTGGC CCCCACCCAG 
GGTGCAGTGT TCGCAGGCTT CTCTGAAGGC ATCTGGAGGG TTCCCAGGGC CAACTGCAGT 
GTCTATAAGA GCTGTGTGGA CTGTGTCCTT GCCAGGGACC CTCACTGTGC CTGGGACCCT 
GAATCAAGAC TGTGCAGCCT TCTGTCTGGC TCTCCCCTGA CTTGGAAGCA GGACATGGAA 
CGCGGCAACC CGGAGTGGGC ATGCGCCCGT GGCCCCATGG CCAGGAGCCC CCGGCGTCAG 
AGCTCCCCTC AACTCATTAA AGAAGTCCTG ACAGTCCCCA ACTCCATCCT GGAGCTGCCC 
TGCCCCCAGC TGTCAGCACT GGCCTCTTAC CACTGGAGTC ACGGCAAAGC CAAAATCCCA 
GAAGCCTCTT CTACTGTCTA TAATGGCTCC CTCTTGTTGC TGCCACAGGA TGGCGTTGGG 
GGCCTCTACC AGTGCGTGGC CACTGAAAAC GGCTATTCGT ACCCTGTAGT CTCCTACTGG 
GTAGACAGCC AGGACCAGCC CCTGGCACTG GATCCTGAGC TGGCGGGCGT TCCCCGGGAG 
CGAGTGCAGG TCCCACTGAC CAGGGTCGGA GGCGGGGCTT CTATGGCTGC CCAGCGGTCC 
TACTGGCCCC ATTTTCTCAT CGTTACTGTC CTCCTGGCCA TTGTGCTCTT GGGAGTGCTC 
ACTCTCCTCC TTGCTTCCCC ACTGGGAGTG CTTCGGGCTC GGGGTAAGGT TCAGGGCTGT 
GGGATGCTGC CCCCCAGGGA AAAGGCCCCA CTGAGCAGGG AACAGCACCT CCAGCCTTCC 
AAGGACCACA GGACCTCTGC CAGCGACGTA GATGCTGACA ACAACCATCT GGGCGCTGAA 
GTGGCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sema4a ( XM_006232640.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006232702.1 Rattus norvegicus semaphorin 4A (Sema4a), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ORa02196
Clone ID Related Accession (Same CDS sequence)	XM_006232640.3 , NM_001012078.2 , XM_006232641.3 , XM_006232639.3
Accession Version	XM_006232640.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2289bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	semaphorin-4A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005101.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006232640.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCATGG CCCTACCATC CCTGGGCCAA GACCCCTGGA GTCTCCTGAG TGTTTTTTTC 
TTCCAACTGT TCCTGCTGCT GTCACTGCCA CCAGCTTCCG GGACTGGTGG GCAGGGACCC 
GTGCCCAGAG TCAAGTACCA TGCGGGAGAT GGGCACAGGG CCCTCAGCTT CTTTCAACAA 
AAAGGCCTCC GGGACTTTGA CACCCTGCTC CTGAGTGGAG ATGGCAACAC TCTGTATGTG 
GGGGCTCGGG AGGCCATCTT GGCCCTGAAT ATCGAGAATC CAGGAATCCC AAGGCTGAGG 
AACATGATAC CCTGGCCAGC CAGTGAGAAA AAAAAGAACG AATGTGCCTT TAAGAAGAAA 
AGCAATGAGA CACAGTGTTT CAACTTCATT CGTGTGCTGG TCTCTTACAA TGCCACTCAC 
CTCTATGCCT GCGGGACTTT TGCCTTCAGC CCCGCCTGCA CCTTCATTGA ACTCCAAGAT 
TCCTTCCTGT TGCCCATCTT GAAGGACAAG GTCACAGATG GGAAGGGTCA AAGCCCCTTT 
GACCCTGCTC ACAAGCACAC AGCTGTCTTG GTCGATGGGA TGCTTTATTC TGGCACCATG 
AACAACTTCC TGGGCAGCGA ACCCATCCTG ATGCGGACAC TGGGATCCCA GCCTGTTCTC 
AAGACTGACA TCTTCCTACG CTGGCTGCAC CCGGACGCCT CCTTCGTGGC AGCCATCCCA 
TCTACCCAGG TCGTCTATTT CTTCTTTGAG GAGACAGCCA GCGAGTTTGA CTTCTTTGAG 
GAGCTGTACA CATCCAGGGT GGCTCAAGTC TGCAAGAACG ACGTAGGCGG TGAAAAGCTG 
CTGCAGAAGA AGTGGACCAC CTTCCTGAAA GCCCAGCTGC TCTGCGCTCA GCCAGGGCAG 
CTGCCATTCA ACATCATCCG CCACGCGGTC CTGCTGCCCG CCGATTCTCC CTCTGTTCCC 
CGCATCTACG CAGTCTTTAC CTCCCAGTGG CAGGTTGGTG GGACCAGGAG CTCAGCAGTC 
TGTGCCTTCT CTCTCACGGA CATTGAGCGA GTCTTTAAAG GAAAGTACAA GGAGCTGAAC 
AAAGAAACCT CCCGCTGGAC CACTTACAGG GGCTCAGAGG TCAACCCGAG GCCAGGCAGC 
TGCTCCATGG GCCCCTCCTC TGACAAAGCC TTGACCTTCA TGAAGGACCA TTTTCTGATG 
GATGAGCATG TGGTAGGAGC TCCCCTGCTG GTGAAGTCTG GCGTGGAGTA CACACAGCTT 
GCCGTGGAAA CAGCTCAGGG CCTTGACGGG AGCAGCCATG TGGTCCTTTA CCTGGGTACC 
TCTACGGGGT CCCTGCACAA AGCTGTGGTG CCTCAGAATA GCAGTGCTTA TCTTGTGGAG 
GAGATTCAGC TGAGCCCTAA CTCTGAGCCT GTTCAGAACC TGCAGCTGGC CCCCACCCAG 
GGTGCAGTGT TCGCAGGCTT CTCTGAAGGC ATCTGGAGGG TTCCCAGGGC CAACTGCAGT 
GTCTATAAGA GCTGTGTGGA CTGTGTCCTT GCCAGGGACC CTCACTGTGC CTGGGACCCT 
GAATCAAGAC TGTGCAGCCT TCTGTCTGGC TCTCCCCTGA CTTGGAAGCA GGACATGGAA 
CGCGGCAACC CGGAGTGGGC ATGCGCCCGT GGCCCCATGG CCAGGAGCCC CCGGCGTCAG 
AGCTCCCCTC AACTCATTAA AGAAGTCCTG ACAGTCCCCA ACTCCATCCT GGAGCTGCCC 
TGCCCCCAGC TGTCAGCACT GGCCTCTTAC CACTGGAGTC ACGGCAAAGC CAAAATCCCA 
GAAGCCTCTT CTACTGTCTA TAATGGCTCC CTCTTGTTGC TGCCACAGGA TGGCGTTGGG 
GGCCTCTACC AGTGCGTGGC CACTGAAAAC GGCTATTCGT ACCCTGTAGT CTCCTACTGG 
GTAGACAGCC AGGACCAGCC CCTGGCACTG GATCCTGAGC TGGCGGGCGT TCCCCGGGAG 
CGAGTGCAGG TCCCACTGAC CAGGGTCGGA GGCGGGGCTT CTATGGCTGC CCAGCGGTCC 
TACTGGCCCC ATTTTCTCAT CGTTACTGTC CTCCTGGCCA TTGTGCTCTT GGGAGTGCTC 
ACTCTCCTCC TTGCTTCCCC ACTGGGAGTG CTTCGGGCTC GGGGTAAGGT TCAGGGCTGT 
GGGATGCTGC CCCCCAGGGA AAAGGCCCCA CTGAGCAGGG AACAGCACCT CCAGCCTTCC 
AAGGACCACA GGACCTCTGC CAGCGACGTA GATGCTGACA ACAACCATCT GGGCGCTGAA 
GTGGCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006232702.1
CDS	95..2383
Translation	MTMALPSLGQDPWSLLSVFFFQLFLLLSLPPASGTGGQGPVPRVKYHAGDGHRALSFFQQKGLRDFDTLLLSGDGNTLYVGAREAILALNIENPGIPRLRNMIPWPASEKKKNECAFKKKSNETQCFNFIRVLVSYNATHLYACGTFAFSPACTFIELQDSFLLPILKDKVTDGKGQSPFDPAHKHTAVLVDGMLYSGTMNNFLGSEPILMRTLGSQPVLKTDIFLRWLHPDASFVAAIPSTQVVYFFFEETASEFDFFEELYTSRVAQVCKNDVGGEKLLQKKWTTFLKAQLLCAQPGQLPFNIIRHAVLLPADSPSVPRIYAVFTSQWQVGGTRSSAVCAFSLTDIERVFKGKYKELNKETSRWTTYRGSEVNPRPGSCSMGPSSDKALTFMKDHFLMDEHVVGAPLLVKSGVEYTQLAVETAQGLDGSSHVVLYLGTSTGSLHKAVVPQNSSAYLVEEIQLSPNSEPVQNLQLAPTQGAVFAGFSEGIWRVPRANCSVYKSCVDCVLARDPHCAWDPESRLCSLLSGSPLTWKQDMERGNPEWACARGPMARSPRRQSSPQLIKEVLTVPNSILELPCPQLSALASYHWSHGKAKIPEASSTVYNGSLLLLPQDGVGGLYQCVATENGYSYPVVSYWVDSQDQPLALDPELAGVPRERVQVPLTRVGGGASMAAQRSYWPHFLIVTVLLAIVLLGVLTLLLASPLGVLRARGKVQGCGMLPPREKAPLSREQHLQPSKDHRTSASDVDADNNHLGAEVA

Target ORF information:

RefSeq Version	XM_006232640.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus semaphorin 4A (Sema4a), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006232640.3

ORF Insert Sequence:

ATGACCATGG CCCTACCATC CCTGGGCCAA GACCCCTGGA GTCTCCTGAG TGTTTTTTTC 
TTCCAACTGT TCCTGCTGCT GTCACTGCCA CCAGCTTCCG GGACTGGTGG GCAGGGACCC 
GTGCCCAGAG TCAAGTACCA TGCGGGAGAT GGGCACAGGG CCCTCAGCTT CTTTCAACAA 
AAAGGCCTCC GGGACTTTGA CACCCTGCTC CTGAGTGGAG ATGGCAACAC TCTGTATGTG 
GGGGCTCGGG AGGCCATCTT GGCCCTGAAT ATCGAGAATC CAGGAATCCC AAGGCTGAGG 
AACATGATAC CCTGGCCAGC CAGTGAGAAA AAAAAGAACG AATGTGCCTT TAAGAAGAAA 
AGCAATGAGA CACAGTGTTT CAACTTCATT CGTGTGCTGG TCTCTTACAA TGCCACTCAC 
CTCTATGCCT GCGGGACTTT TGCCTTCAGC CCCGCCTGCA CCTTCATTGA ACTCCAAGAT 
TCCTTCCTGT TGCCCATCTT GAAGGACAAG GTCACAGATG GGAAGGGTCA AAGCCCCTTT 
GACCCTGCTC ACAAGCACAC AGCTGTCTTG GTCGATGGGA TGCTTTATTC TGGCACCATG 
AACAACTTCC TGGGCAGCGA ACCCATCCTG ATGCGGACAC TGGGATCCCA GCCTGTTCTC 
AAGACTGACA TCTTCCTACG CTGGCTGCAC CCGGACGCCT CCTTCGTGGC AGCCATCCCA 
TCTACCCAGG TCGTCTATTT CTTCTTTGAG GAGACAGCCA GCGAGTTTGA CTTCTTTGAG 
GAGCTGTACA CATCCAGGGT GGCTCAAGTC TGCAAGAACG ACGTAGGCGG TGAAAAGCTG 
CTGCAGAAGA AGTGGACCAC CTTCCTGAAA GCCCAGCTGC TCTGCGCTCA GCCAGGGCAG 
CTGCCATTCA ACATCATCCG CCACGCGGTC CTGCTGCCCG CCGATTCTCC CTCTGTTCCC 
CGCATCTACG CAGTCTTTAC CTCCCAGTGG CAGGTTGGTG GGACCAGGAG CTCAGCAGTC 
TGTGCCTTCT CTCTCACGGA CATTGAGCGA GTCTTTAAAG GAAAGTACAA GGAGCTGAAC 
AAAGAAACCT CCCGCTGGAC CACTTACAGG GGCTCAGAGG TCAACCCGAG GCCAGGCAGC 
TGCTCCATGG GCCCCTCCTC TGACAAAGCC TTGACCTTCA TGAAGGACCA TTTTCTGATG 
GATGAGCATG TGGTAGGAGC TCCCCTGCTG GTGAAGTCTG GCGTGGAGTA CACACAGCTT 
GCCGTGGAAA CAGCTCAGGG CCTTGACGGG AGCAGCCATG TGGTCCTTTA CCTGGGTACC 
TCTACGGGGT CCCTGCACAA AGCTGTGGTG CCTCAGAATA GCAGTGCTTA TCTTGTGGAG 
GAGATTCAGC TGAGCCCTAA CTCTGAGCCT GTTCAGAACC TGCAGCTGGC CCCCACCCAG 
GGTGCAGTGT TCGCAGGCTT CTCTGAAGGC ATCTGGAGGG TTCCCAGGGC CAACTGCAGT 
GTCTATAAGA GCTGTGTGGA CTGTGTCCTT GCCAGGGACC CTCACTGTGC CTGGGACCCT 
GAATCAAGAC TGTGCAGCCT TCTGTCTGGC TCTCCCCTGA CTTGGAAGCA GGACATGGAA 
CGCGGCAACC CGGAGTGGGC ATGCGCCCGT GGCCCCATGG CCAGGAGCCC CCGGCGTCAG 
AGCTCCCCTC AACTCATTAA AGAAGTCCTG ACAGTCCCCA ACTCCATCCT GGAGCTGCCC 
TGCCCCCAGC TGTCAGCACT GGCCTCTTAC CACTGGAGTC ACGGCAAAGC CAAAATCCCA 
GAAGCCTCTT CTACTGTCTA TAATGGCTCC CTCTTGTTGC TGCCACAGGA TGGCGTTGGG 
GGCCTCTACC AGTGCGTGGC CACTGAAAAC GGCTATTCGT ACCCTGTAGT CTCCTACTGG 
GTAGACAGCC AGGACCAGCC CCTGGCACTG GATCCTGAGC TGGCGGGCGT TCCCCGGGAG 
CGAGTGCAGG TCCCACTGAC CAGGGTCGGA GGCGGGGCTT CTATGGCTGC CCAGCGGTCC 
TACTGGCCCC ATTTTCTCAT CGTTACTGTC CTCCTGGCCA TTGTGCTCTT GGGAGTGCTC 
ACTCTCCTCC TTGCTTCCCC ACTGGGAGTG CTTCGGGCTC GGGGTAAGGT TCAGGGCTGT 
GGGATGCTGC CCCCCAGGGA AAAGGCCCCA CTGAGCAGGG AACAGCACCT CCAGCCTTCC 
AAGGACCACA GGACCTCTGC CAGCGACGTA GATGCTGACA ACAACCATCT GGGCGCTGAA 
GTGGCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sema4a ( XM_006232641.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006232703.1 Rattus norvegicus semaphorin 4A (Sema4a), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa02196
Clone ID Related Accession (Same CDS sequence)	XM_006232640.3 , NM_001012078.2 , XM_006232641.3 , XM_006232639.3
Accession Version	XM_006232641.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2289bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	semaphorin-4A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005101.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006232641.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCATGG CCCTACCATC CCTGGGCCAA GACCCCTGGA GTCTCCTGAG TGTTTTTTTC 
TTCCAACTGT TCCTGCTGCT GTCACTGCCA CCAGCTTCCG GGACTGGTGG GCAGGGACCC 
GTGCCCAGAG TCAAGTACCA TGCGGGAGAT GGGCACAGGG CCCTCAGCTT CTTTCAACAA 
AAAGGCCTCC GGGACTTTGA CACCCTGCTC CTGAGTGGAG ATGGCAACAC TCTGTATGTG 
GGGGCTCGGG AGGCCATCTT GGCCCTGAAT ATCGAGAATC CAGGAATCCC AAGGCTGAGG 
AACATGATAC CCTGGCCAGC CAGTGAGAAA AAAAAGAACG AATGTGCCTT TAAGAAGAAA 
AGCAATGAGA CACAGTGTTT CAACTTCATT CGTGTGCTGG TCTCTTACAA TGCCACTCAC 
CTCTATGCCT GCGGGACTTT TGCCTTCAGC CCCGCCTGCA CCTTCATTGA ACTCCAAGAT 
TCCTTCCTGT TGCCCATCTT GAAGGACAAG GTCACAGATG GGAAGGGTCA AAGCCCCTTT 
GACCCTGCTC ACAAGCACAC AGCTGTCTTG GTCGATGGGA TGCTTTATTC TGGCACCATG 
AACAACTTCC TGGGCAGCGA ACCCATCCTG ATGCGGACAC TGGGATCCCA GCCTGTTCTC 
AAGACTGACA TCTTCCTACG CTGGCTGCAC CCGGACGCCT CCTTCGTGGC AGCCATCCCA 
TCTACCCAGG TCGTCTATTT CTTCTTTGAG GAGACAGCCA GCGAGTTTGA CTTCTTTGAG 
GAGCTGTACA CATCCAGGGT GGCTCAAGTC TGCAAGAACG ACGTAGGCGG TGAAAAGCTG 
CTGCAGAAGA AGTGGACCAC CTTCCTGAAA GCCCAGCTGC TCTGCGCTCA GCCAGGGCAG 
CTGCCATTCA ACATCATCCG CCACGCGGTC CTGCTGCCCG CCGATTCTCC CTCTGTTCCC 
CGCATCTACG CAGTCTTTAC CTCCCAGTGG CAGGTTGGTG GGACCAGGAG CTCAGCAGTC 
TGTGCCTTCT CTCTCACGGA CATTGAGCGA GTCTTTAAAG GAAAGTACAA GGAGCTGAAC 
AAAGAAACCT CCCGCTGGAC CACTTACAGG GGCTCAGAGG TCAACCCGAG GCCAGGCAGC 
TGCTCCATGG GCCCCTCCTC TGACAAAGCC TTGACCTTCA TGAAGGACCA TTTTCTGATG 
GATGAGCATG TGGTAGGAGC TCCCCTGCTG GTGAAGTCTG GCGTGGAGTA CACACAGCTT 
GCCGTGGAAA CAGCTCAGGG CCTTGACGGG AGCAGCCATG TGGTCCTTTA CCTGGGTACC 
TCTACGGGGT CCCTGCACAA AGCTGTGGTG CCTCAGAATA GCAGTGCTTA TCTTGTGGAG 
GAGATTCAGC TGAGCCCTAA CTCTGAGCCT GTTCAGAACC TGCAGCTGGC CCCCACCCAG 
GGTGCAGTGT TCGCAGGCTT CTCTGAAGGC ATCTGGAGGG TTCCCAGGGC CAACTGCAGT 
GTCTATAAGA GCTGTGTGGA CTGTGTCCTT GCCAGGGACC CTCACTGTGC CTGGGACCCT 
GAATCAAGAC TGTGCAGCCT TCTGTCTGGC TCTCCCCTGA CTTGGAAGCA GGACATGGAA 
CGCGGCAACC CGGAGTGGGC ATGCGCCCGT GGCCCCATGG CCAGGAGCCC CCGGCGTCAG 
AGCTCCCCTC AACTCATTAA AGAAGTCCTG ACAGTCCCCA ACTCCATCCT GGAGCTGCCC 
TGCCCCCAGC TGTCAGCACT GGCCTCTTAC CACTGGAGTC ACGGCAAAGC CAAAATCCCA 
GAAGCCTCTT CTACTGTCTA TAATGGCTCC CTCTTGTTGC TGCCACAGGA TGGCGTTGGG 
GGCCTCTACC AGTGCGTGGC CACTGAAAAC GGCTATTCGT ACCCTGTAGT CTCCTACTGG 
GTAGACAGCC AGGACCAGCC CCTGGCACTG GATCCTGAGC TGGCGGGCGT TCCCCGGGAG 
CGAGTGCAGG TCCCACTGAC CAGGGTCGGA GGCGGGGCTT CTATGGCTGC CCAGCGGTCC 
TACTGGCCCC ATTTTCTCAT CGTTACTGTC CTCCTGGCCA TTGTGCTCTT GGGAGTGCTC 
ACTCTCCTCC TTGCTTCCCC ACTGGGAGTG CTTCGGGCTC GGGGTAAGGT TCAGGGCTGT 
GGGATGCTGC CCCCCAGGGA AAAGGCCCCA CTGAGCAGGG AACAGCACCT CCAGCCTTCC 
AAGGACCACA GGACCTCTGC CAGCGACGTA GATGCTGACA ACAACCATCT GGGCGCTGAA 
GTGGCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006232703.1
CDS	303..2591
Translation	MTMALPSLGQDPWSLLSVFFFQLFLLLSLPPASGTGGQGPVPRVKYHAGDGHRALSFFQQKGLRDFDTLLLSGDGNTLYVGAREAILALNIENPGIPRLRNMIPWPASEKKKNECAFKKKSNETQCFNFIRVLVSYNATHLYACGTFAFSPACTFIELQDSFLLPILKDKVTDGKGQSPFDPAHKHTAVLVDGMLYSGTMNNFLGSEPILMRTLGSQPVLKTDIFLRWLHPDASFVAAIPSTQVVYFFFEETASEFDFFEELYTSRVAQVCKNDVGGEKLLQKKWTTFLKAQLLCAQPGQLPFNIIRHAVLLPADSPSVPRIYAVFTSQWQVGGTRSSAVCAFSLTDIERVFKGKYKELNKETSRWTTYRGSEVNPRPGSCSMGPSSDKALTFMKDHFLMDEHVVGAPLLVKSGVEYTQLAVETAQGLDGSSHVVLYLGTSTGSLHKAVVPQNSSAYLVEEIQLSPNSEPVQNLQLAPTQGAVFAGFSEGIWRVPRANCSVYKSCVDCVLARDPHCAWDPESRLCSLLSGSPLTWKQDMERGNPEWACARGPMARSPRRQSSPQLIKEVLTVPNSILELPCPQLSALASYHWSHGKAKIPEASSTVYNGSLLLLPQDGVGGLYQCVATENGYSYPVVSYWVDSQDQPLALDPELAGVPRERVQVPLTRVGGGASMAAQRSYWPHFLIVTVLLAIVLLGVLTLLLASPLGVLRARGKVQGCGMLPPREKAPLSREQHLQPSKDHRTSASDVDADNNHLGAEVA

Target ORF information:

RefSeq Version	XM_006232641.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus semaphorin 4A (Sema4a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006232641.3

ORF Insert Sequence:

ATGACCATGG CCCTACCATC CCTGGGCCAA GACCCCTGGA GTCTCCTGAG TGTTTTTTTC 
TTCCAACTGT TCCTGCTGCT GTCACTGCCA CCAGCTTCCG GGACTGGTGG GCAGGGACCC 
GTGCCCAGAG TCAAGTACCA TGCGGGAGAT GGGCACAGGG CCCTCAGCTT CTTTCAACAA 
AAAGGCCTCC GGGACTTTGA CACCCTGCTC CTGAGTGGAG ATGGCAACAC TCTGTATGTG 
GGGGCTCGGG AGGCCATCTT GGCCCTGAAT ATCGAGAATC CAGGAATCCC AAGGCTGAGG 
AACATGATAC CCTGGCCAGC CAGTGAGAAA AAAAAGAACG AATGTGCCTT TAAGAAGAAA 
AGCAATGAGA CACAGTGTTT CAACTTCATT CGTGTGCTGG TCTCTTACAA TGCCACTCAC 
CTCTATGCCT GCGGGACTTT TGCCTTCAGC CCCGCCTGCA CCTTCATTGA ACTCCAAGAT 
TCCTTCCTGT TGCCCATCTT GAAGGACAAG GTCACAGATG GGAAGGGTCA AAGCCCCTTT 
GACCCTGCTC ACAAGCACAC AGCTGTCTTG GTCGATGGGA TGCTTTATTC TGGCACCATG 
AACAACTTCC TGGGCAGCGA ACCCATCCTG ATGCGGACAC TGGGATCCCA GCCTGTTCTC 
AAGACTGACA TCTTCCTACG CTGGCTGCAC CCGGACGCCT CCTTCGTGGC AGCCATCCCA 
TCTACCCAGG TCGTCTATTT CTTCTTTGAG GAGACAGCCA GCGAGTTTGA CTTCTTTGAG 
GAGCTGTACA CATCCAGGGT GGCTCAAGTC TGCAAGAACG ACGTAGGCGG TGAAAAGCTG 
CTGCAGAAGA AGTGGACCAC CTTCCTGAAA GCCCAGCTGC TCTGCGCTCA GCCAGGGCAG 
CTGCCATTCA ACATCATCCG CCACGCGGTC CTGCTGCCCG CCGATTCTCC CTCTGTTCCC 
CGCATCTACG CAGTCTTTAC CTCCCAGTGG CAGGTTGGTG GGACCAGGAG CTCAGCAGTC 
TGTGCCTTCT CTCTCACGGA CATTGAGCGA GTCTTTAAAG GAAAGTACAA GGAGCTGAAC 
AAAGAAACCT CCCGCTGGAC CACTTACAGG GGCTCAGAGG TCAACCCGAG GCCAGGCAGC 
TGCTCCATGG GCCCCTCCTC TGACAAAGCC TTGACCTTCA TGAAGGACCA TTTTCTGATG 
GATGAGCATG TGGTAGGAGC TCCCCTGCTG GTGAAGTCTG GCGTGGAGTA CACACAGCTT 
GCCGTGGAAA CAGCTCAGGG CCTTGACGGG AGCAGCCATG TGGTCCTTTA CCTGGGTACC 
TCTACGGGGT CCCTGCACAA AGCTGTGGTG CCTCAGAATA GCAGTGCTTA TCTTGTGGAG 
GAGATTCAGC TGAGCCCTAA CTCTGAGCCT GTTCAGAACC TGCAGCTGGC CCCCACCCAG 
GGTGCAGTGT TCGCAGGCTT CTCTGAAGGC ATCTGGAGGG TTCCCAGGGC CAACTGCAGT 
GTCTATAAGA GCTGTGTGGA CTGTGTCCTT GCCAGGGACC CTCACTGTGC CTGGGACCCT 
GAATCAAGAC TGTGCAGCCT TCTGTCTGGC TCTCCCCTGA CTTGGAAGCA GGACATGGAA 
CGCGGCAACC CGGAGTGGGC ATGCGCCCGT GGCCCCATGG CCAGGAGCCC CCGGCGTCAG 
AGCTCCCCTC AACTCATTAA AGAAGTCCTG ACAGTCCCCA ACTCCATCCT GGAGCTGCCC 
TGCCCCCAGC TGTCAGCACT GGCCTCTTAC CACTGGAGTC ACGGCAAAGC CAAAATCCCA 
GAAGCCTCTT CTACTGTCTA TAATGGCTCC CTCTTGTTGC TGCCACAGGA TGGCGTTGGG 
GGCCTCTACC AGTGCGTGGC CACTGAAAAC GGCTATTCGT ACCCTGTAGT CTCCTACTGG 
GTAGACAGCC AGGACCAGCC CCTGGCACTG GATCCTGAGC TGGCGGGCGT TCCCCGGGAG 
CGAGTGCAGG TCCCACTGAC CAGGGTCGGA GGCGGGGCTT CTATGGCTGC CCAGCGGTCC 
TACTGGCCCC ATTTTCTCAT CGTTACTGTC CTCCTGGCCA TTGTGCTCTT GGGAGTGCTC 
ACTCTCCTCC TTGCTTCCCC ACTGGGAGTG CTTCGGGCTC GGGGTAAGGT TCAGGGCTGT 
GGGATGCTGC CCCCCAGGGA AAAGGCCCCA CTGAGCAGGG AACAGCACCT CCAGCCTTCC 
AAGGACCACA GGACCTCTGC CAGCGACGTA GATGCTGACA ACAACCATCT GGGCGCTGAA 
GTGGCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Sema4a ( XM_006232639.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006232701.1 Rattus norvegicus semaphorin 4A (Sema4a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa02196
Clone ID Related Accession (Same CDS sequence)	XM_006232640.3 , NM_001012078.2 , XM_006232641.3 , XM_006232639.3
Accession Version	XM_006232639.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2289bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	semaphorin-4A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005101.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006232639.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACCATGG CCCTACCATC CCTGGGCCAA GACCCCTGGA GTCTCCTGAG TGTTTTTTTC 
TTCCAACTGT TCCTGCTGCT GTCACTGCCA CCAGCTTCCG GGACTGGTGG GCAGGGACCC 
GTGCCCAGAG TCAAGTACCA TGCGGGAGAT GGGCACAGGG CCCTCAGCTT CTTTCAACAA 
AAAGGCCTCC GGGACTTTGA CACCCTGCTC CTGAGTGGAG ATGGCAACAC TCTGTATGTG 
GGGGCTCGGG AGGCCATCTT GGCCCTGAAT ATCGAGAATC CAGGAATCCC AAGGCTGAGG 
AACATGATAC CCTGGCCAGC CAGTGAGAAA AAAAAGAACG AATGTGCCTT TAAGAAGAAA 
AGCAATGAGA CACAGTGTTT CAACTTCATT CGTGTGCTGG TCTCTTACAA TGCCACTCAC 
CTCTATGCCT GCGGGACTTT TGCCTTCAGC CCCGCCTGCA CCTTCATTGA ACTCCAAGAT 
TCCTTCCTGT TGCCCATCTT GAAGGACAAG GTCACAGATG GGAAGGGTCA AAGCCCCTTT 
GACCCTGCTC ACAAGCACAC AGCTGTCTTG GTCGATGGGA TGCTTTATTC TGGCACCATG 
AACAACTTCC TGGGCAGCGA ACCCATCCTG ATGCGGACAC TGGGATCCCA GCCTGTTCTC 
AAGACTGACA TCTTCCTACG CTGGCTGCAC CCGGACGCCT CCTTCGTGGC AGCCATCCCA 
TCTACCCAGG TCGTCTATTT CTTCTTTGAG GAGACAGCCA GCGAGTTTGA CTTCTTTGAG 
GAGCTGTACA CATCCAGGGT GGCTCAAGTC TGCAAGAACG ACGTAGGCGG TGAAAAGCTG 
CTGCAGAAGA AGTGGACCAC CTTCCTGAAA GCCCAGCTGC TCTGCGCTCA GCCAGGGCAG 
CTGCCATTCA ACATCATCCG CCACGCGGTC CTGCTGCCCG CCGATTCTCC CTCTGTTCCC 
CGCATCTACG CAGTCTTTAC CTCCCAGTGG CAGGTTGGTG GGACCAGGAG CTCAGCAGTC 
TGTGCCTTCT CTCTCACGGA CATTGAGCGA GTCTTTAAAG GAAAGTACAA GGAGCTGAAC 
AAAGAAACCT CCCGCTGGAC CACTTACAGG GGCTCAGAGG TCAACCCGAG GCCAGGCAGC 
TGCTCCATGG GCCCCTCCTC TGACAAAGCC TTGACCTTCA TGAAGGACCA TTTTCTGATG 
GATGAGCATG TGGTAGGAGC TCCCCTGCTG GTGAAGTCTG GCGTGGAGTA CACACAGCTT 
GCCGTGGAAA CAGCTCAGGG CCTTGACGGG AGCAGCCATG TGGTCCTTTA CCTGGGTACC 
TCTACGGGGT CCCTGCACAA AGCTGTGGTG CCTCAGAATA GCAGTGCTTA TCTTGTGGAG 
GAGATTCAGC TGAGCCCTAA CTCTGAGCCT GTTCAGAACC TGCAGCTGGC CCCCACCCAG 
GGTGCAGTGT TCGCAGGCTT CTCTGAAGGC ATCTGGAGGG TTCCCAGGGC CAACTGCAGT 
GTCTATAAGA GCTGTGTGGA CTGTGTCCTT GCCAGGGACC CTCACTGTGC CTGGGACCCT 
GAATCAAGAC TGTGCAGCCT TCTGTCTGGC TCTCCCCTGA CTTGGAAGCA GGACATGGAA 
CGCGGCAACC CGGAGTGGGC ATGCGCCCGT GGCCCCATGG CCAGGAGCCC CCGGCGTCAG 
AGCTCCCCTC AACTCATTAA AGAAGTCCTG ACAGTCCCCA ACTCCATCCT GGAGCTGCCC 
TGCCCCCAGC TGTCAGCACT GGCCTCTTAC CACTGGAGTC ACGGCAAAGC CAAAATCCCA 
GAAGCCTCTT CTACTGTCTA TAATGGCTCC CTCTTGTTGC TGCCACAGGA TGGCGTTGGG 
GGCCTCTACC AGTGCGTGGC CACTGAAAAC GGCTATTCGT ACCCTGTAGT CTCCTACTGG 
GTAGACAGCC AGGACCAGCC CCTGGCACTG GATCCTGAGC TGGCGGGCGT TCCCCGGGAG 
CGAGTGCAGG TCCCACTGAC CAGGGTCGGA GGCGGGGCTT CTATGGCTGC CCAGCGGTCC 
TACTGGCCCC ATTTTCTCAT CGTTACTGTC CTCCTGGCCA TTGTGCTCTT GGGAGTGCTC 
ACTCTCCTCC TTGCTTCCCC ACTGGGAGTG CTTCGGGCTC GGGGTAAGGT TCAGGGCTGT 
GGGATGCTGC CCCCCAGGGA AAAGGCCCCA CTGAGCAGGG AACAGCACCT CCAGCCTTCC 
AAGGACCACA GGACCTCTGC CAGCGACGTA GATGCTGACA ACAACCATCT GGGCGCTGAA 
GTGGCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006232701.1
CDS	127..2415
Translation	MTMALPSLGQDPWSLLSVFFFQLFLLLSLPPASGTGGQGPVPRVKYHAGDGHRALSFFQQKGLRDFDTLLLSGDGNTLYVGAREAILALNIENPGIPRLRNMIPWPASEKKKNECAFKKKSNETQCFNFIRVLVSYNATHLYACGTFAFSPACTFIELQDSFLLPILKDKVTDGKGQSPFDPAHKHTAVLVDGMLYSGTMNNFLGSEPILMRTLGSQPVLKTDIFLRWLHPDASFVAAIPSTQVVYFFFEETASEFDFFEELYTSRVAQVCKNDVGGEKLLQKKWTTFLKAQLLCAQPGQLPFNIIRHAVLLPADSPSVPRIYAVFTSQWQVGGTRSSAVCAFSLTDIERVFKGKYKELNKETSRWTTYRGSEVNPRPGSCSMGPSSDKALTFMKDHFLMDEHVVGAPLLVKSGVEYTQLAVETAQGLDGSSHVVLYLGTSTGSLHKAVVPQNSSAYLVEEIQLSPNSEPVQNLQLAPTQGAVFAGFSEGIWRVPRANCSVYKSCVDCVLARDPHCAWDPESRLCSLLSGSPLTWKQDMERGNPEWACARGPMARSPRRQSSPQLIKEVLTVPNSILELPCPQLSALASYHWSHGKAKIPEASSTVYNGSLLLLPQDGVGGLYQCVATENGYSYPVVSYWVDSQDQPLALDPELAGVPRERVQVPLTRVGGGASMAAQRSYWPHFLIVTVLLAIVLLGVLTLLLASPLGVLRARGKVQGCGMLPPREKAPLSREQHLQPSKDHRTSASDVDADNNHLGAEVA

Target ORF information:

RefSeq Version	XM_006232639.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus semaphorin 4A (Sema4a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006232639.3

ORF Insert Sequence:

ATGACCATGG CCCTACCATC CCTGGGCCAA GACCCCTGGA GTCTCCTGAG TGTTTTTTTC 
TTCCAACTGT TCCTGCTGCT GTCACTGCCA CCAGCTTCCG GGACTGGTGG GCAGGGACCC 
GTGCCCAGAG TCAAGTACCA TGCGGGAGAT GGGCACAGGG CCCTCAGCTT CTTTCAACAA 
AAAGGCCTCC GGGACTTTGA CACCCTGCTC CTGAGTGGAG ATGGCAACAC TCTGTATGTG 
GGGGCTCGGG AGGCCATCTT GGCCCTGAAT ATCGAGAATC CAGGAATCCC AAGGCTGAGG 
AACATGATAC CCTGGCCAGC CAGTGAGAAA AAAAAGAACG AATGTGCCTT TAAGAAGAAA 
AGCAATGAGA CACAGTGTTT CAACTTCATT CGTGTGCTGG TCTCTTACAA TGCCACTCAC 
CTCTATGCCT GCGGGACTTT TGCCTTCAGC CCCGCCTGCA CCTTCATTGA ACTCCAAGAT 
TCCTTCCTGT TGCCCATCTT GAAGGACAAG GTCACAGATG GGAAGGGTCA AAGCCCCTTT 
GACCCTGCTC ACAAGCACAC AGCTGTCTTG GTCGATGGGA TGCTTTATTC TGGCACCATG 
AACAACTTCC TGGGCAGCGA ACCCATCCTG ATGCGGACAC TGGGATCCCA GCCTGTTCTC 
AAGACTGACA TCTTCCTACG CTGGCTGCAC CCGGACGCCT CCTTCGTGGC AGCCATCCCA 
TCTACCCAGG TCGTCTATTT CTTCTTTGAG GAGACAGCCA GCGAGTTTGA CTTCTTTGAG 
GAGCTGTACA CATCCAGGGT GGCTCAAGTC TGCAAGAACG ACGTAGGCGG TGAAAAGCTG 
CTGCAGAAGA AGTGGACCAC CTTCCTGAAA GCCCAGCTGC TCTGCGCTCA GCCAGGGCAG 
CTGCCATTCA ACATCATCCG CCACGCGGTC CTGCTGCCCG CCGATTCTCC CTCTGTTCCC 
CGCATCTACG CAGTCTTTAC CTCCCAGTGG CAGGTTGGTG GGACCAGGAG CTCAGCAGTC 
TGTGCCTTCT CTCTCACGGA CATTGAGCGA GTCTTTAAAG GAAAGTACAA GGAGCTGAAC 
AAAGAAACCT CCCGCTGGAC CACTTACAGG GGCTCAGAGG TCAACCCGAG GCCAGGCAGC 
TGCTCCATGG GCCCCTCCTC TGACAAAGCC TTGACCTTCA TGAAGGACCA TTTTCTGATG 
GATGAGCATG TGGTAGGAGC TCCCCTGCTG GTGAAGTCTG GCGTGGAGTA CACACAGCTT 
GCCGTGGAAA CAGCTCAGGG CCTTGACGGG AGCAGCCATG TGGTCCTTTA CCTGGGTACC 
TCTACGGGGT CCCTGCACAA AGCTGTGGTG CCTCAGAATA GCAGTGCTTA TCTTGTGGAG 
GAGATTCAGC TGAGCCCTAA CTCTGAGCCT GTTCAGAACC TGCAGCTGGC CCCCACCCAG 
GGTGCAGTGT TCGCAGGCTT CTCTGAAGGC ATCTGGAGGG TTCCCAGGGC CAACTGCAGT 
GTCTATAAGA GCTGTGTGGA CTGTGTCCTT GCCAGGGACC CTCACTGTGC CTGGGACCCT 
GAATCAAGAC TGTGCAGCCT TCTGTCTGGC TCTCCCCTGA CTTGGAAGCA GGACATGGAA 
CGCGGCAACC CGGAGTGGGC ATGCGCCCGT GGCCCCATGG CCAGGAGCCC CCGGCGTCAG 
AGCTCCCCTC AACTCATTAA AGAAGTCCTG ACAGTCCCCA ACTCCATCCT GGAGCTGCCC 
TGCCCCCAGC TGTCAGCACT GGCCTCTTAC CACTGGAGTC ACGGCAAAGC CAAAATCCCA 
GAAGCCTCTT CTACTGTCTA TAATGGCTCC CTCTTGTTGC TGCCACAGGA TGGCGTTGGG 
GGCCTCTACC AGTGCGTGGC CACTGAAAAC GGCTATTCGT ACCCTGTAGT CTCCTACTGG 
GTAGACAGCC AGGACCAGCC CCTGGCACTG GATCCTGAGC TGGCGGGCGT TCCCCGGGAG 
CGAGTGCAGG TCCCACTGAC CAGGGTCGGA GGCGGGGCTT CTATGGCTGC CCAGCGGTCC 
TACTGGCCCC ATTTTCTCAT CGTTACTGTC CTCCTGGCCA TTGTGCTCTT GGGAGTGCTC 
ACTCTCCTCC TTGCTTCCCC ACTGGGAGTG CTTCGGGCTC GGGGTAAGGT TCAGGGCTGT 
GGGATGCTGC CCCCCAGGGA AAAGGCCCCA CTGAGCAGGG AACAGCACCT CCAGCCTTCC 
AAGGACCACA GGACCTCTGC CAGCGACGTA GATGCTGACA ACAACCATCT GGGCGCTGAA 
GTGGCTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sema4A induces cell morphological changes through B-type plexin-mediated signaling.
International journal of molecular medicine25(2)225-30(2010 Feb)
Yukawa K,Tanaka T,Yoshida K,Takeuchi N,Ito T,Takamatsu H,Kikutani H,Kumanogoh A

Semaphorin-4A, an activator for T-cell-mediated immunity, suppresses angiogenesis via Plexin-D1.
The EMBO journal26(5)1373-84(2007 Mar)
Toyofuku T,Yabuki M,Kamei J,Kamei M,Makino N,Kumanogoh A,Hori M

Nonredundant roles of Sema4A in the immune system: defective T cell priming and Th1/Th2 regulation in Sema4A-deficient mice.
Immunity22(3)305-16(2005 Mar)
Kumanogoh A,Shikina T,Suzuki K,Uematsu S,Yukawa K,Kashiwamura S,Tsutsui H,Yamamoto M,Takamatsu H,Ko-Mitamura EP,Takegahara N,Marukawa S,Ishida I,Morishita H,Prasad DV,Tamura M,Mizui M,Toyofuku T,Akira S,Takeda K,Okabe M,Kikutani H

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Sema4a cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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