Sema5a cDNA ORF clone, Rattus norvegicus(Norway rat)

The following Sema5a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Sema5a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ORa05493 NM_001107659.2
Latest version!
Rattus norvegicus semaphorin 5A (Sema5a), mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $559.30
$799.00
ORa05493 XM_017590833.1
Latest version!
Rattus norvegicus semaphorin 5A (Sema5a), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $559.30
$799.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ORa05493
    Clone ID Related Accession (Same CDS sequence) XM_017590833.1 , NM_001107659.2
    Accession Version NM_001107659.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3225bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-09-23
    Organism Rattus norvegicus(Norway rat)
    Product semaphorin-5A precursor
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AABR07009051.1 and AABR07009052.1. On Jan 9, 2008 this sequence version replaced NM_001107659.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGAAGGGAG CCTGCATCCT TGCATGGCTG TTCTCAAGCC TGGGAGTGTG GAGACTTGCT 
    CGGCCTGAGA CCCAGGACCC TGCCAAGTGC CAGAGAGCTG AGCACCCCGT CGTCTCCTAC
    AAAGAAATTG GCCCCTGGTT ACGGGAATTC AGAGCCGAGA ATGCTGTGGA TTTCTCGAGG
    TTAACATTTG ACCCAGGACA GAAAGAACTT GTCGTAGGAG CAAGAAACTA TCTCTTCAGA
    CTACAGCTCG AGGATCTGTC TCTCATCCAG GCTGTGCAGT GGGAGTGTGA TGAAGCCACG
    AAGAAGGCCT GTTACAGCAA GGGCAAATCG AAGGAGGAAT GTCAGAACTA CATACGAGTG
    CTCTTAGTGG GCGGGGACCG GCTCTTCACC TGTGGGACCA ATGCCTTCAC ACCCGTCTGT
    ACCATCCGCT CCTTAAGTAA CCTGACTGAG ATCCATGATC AGATCAGTGG CATGGCTCGA
    TGTCCCTATA GTCCCCAGCA CAATTCTACA GCTCTACTCA CTGCCAGTGG CGAGCTCTAT
    GCTGCAACAG CCATGGATTT CCCAGGGCGC GATCCAGCCA TTTATCGAAG TCTGGGCACT
    TTACCTCCTC TTCGAACTGC ACAGTACAAC TCCAAATGGC TCAATGAACC AAACTTTGTG
    TCTTCCTACG ACATCGGGAA CTTCACCTAC TTCTTCTTCC GAGAAAATGC CGTGGAACAC
    GACTGTGGGA AGACTGTGTT CTCCAGGGCA GCCAGGGTCT GCAAGAATGA CATTGGAGGA
    CGATTTCTTC TGGAGGATAC CTGGACTACC TTCATGAAGG CTCGCCTCAA CTGCTCTCGG
    CCTGGTGAGG TGCCGTTCTA CTACAACGAG CTTCAAAGCA CCTTCTTCCT GCCGGAGCTG
    GATCTGATCT ACGGCATCTT CACCACCAAT GTAAACAGCA TTGCTGCCTC TGCTGTCTGT
    GTCTTCAACC TGAGCGCCAT CTCACAGGCC TTCAATGGAC CCTTCAAGTA CCAAGAGAAC
    TCTCGCTCAG CCTGGCTGCC TTATCCCAAC CCCAACCCCA ATTTTCAGTG CGGCACCATG
    GACCAGGGCC TGTATGTAAA CCTGACGGAA AGAAACCTAC AGGATGCTCA GAAGTTCATC
    CTGATGCATG AAGTGGTGCA GCCAGTGACC ACAGTGCCTT CCTTCATGGA AGACAACAGC
    CGTTTCTCCC ATGTGGCTGT CGATGTTGTG CAAGGCAGGG ACACCCTTGT CCATATCATC
    TATCTGGCCA CAGATTATGG CACCATTAAA AAAGTGCGGG CTCCGCTGAG TCAGAGCTCG
    GGCAGCTGTT TGCTAGAAGA GATTGAGCTT TTTCCAGAGA GGAAGAGTGA GCCCATCAGG
    AGCCTAAAGA TCCTCCACAG CCAGAGTGTC CTATTTGTGG GGCTACAGGA GCATGTGGTC
    AAGATCCCCC TGAAGAGATG CCATTTTCAT CAAACACGTG GTGCCTGCAT TGGTGCTCAG
    GACCCTTACT GTGGCTGGGA TGCGGTGATG AAGAAGTGCA CCAGCCTGGA GGAGAGCCTG
    AGCATGACCC AGTGGGATCA GAGCGTCCCC ACCTGTCCGA CCAGAAATCT CACCGTAGAT
    GGGAGCTTTG GCCCGTGGTC TCCATGGACA CCCTGTACTC ACACTGATGG CACTGCTGTG
    GGATCCTGCC TCTGCCGGTC CCGATCCTGT GACAGCCCAG CTCCACAGTG TGGCGGCTGG
    CAGTGTGAGG GCCCTAGAAT GGAGATCACC AACTGTTCCA GGAATGGAGG CTGGACTCCC
    TGGACCTCCT GGTCTCCCTG CAGCACCACT TGTGGCATTG GCTTCCAGGT GCGGCAGCGC
    TCCTGCAGCA ACCCTACGCC CAGGCATGGA GGGCGTGTGT GCGTGGGCCA GAACAGGGAG
    GAAAGATACT GCAATGAACA TTTACTCTGC CCACCACACG TGTTCTGGAC AGGCTGGGGA
    CCTTGGGAAC GCTGCACAGC CCAGTGCGGG GGTGGCATTC AAGCTCGCCG TAGGACCTGT
    GAAAATGGGC CTGACTGTGC AGGATGCAAT GTGGAATACC AGCCTTGTAA CACCAATGCA
    TGCCCAGAGT TGAAGAAGAC CACACCATGG ACCCCCTGGA CACCTGTCAA CATCTCTGAC
    AATGGGGGCC ACTATGAGCA GCGGTTCCGC TACACCTGTA AAGCTCGCCT GCCAGATCCA
    AACTTATTGG AAGTAGGAAG ACAAAGGATA GAAATGCGGT ACTGTTCTAG TGATGGGACC
    AGTGGCTGCT CCACAGATGG ACTTTCTGGA GACTTTCTAA GAGCTGGGAG ATACTCTGCC
    CATACAGTCA ATGGGGCATG GTCAGCCTGG ACTTCCTGGT CACAGTGCAG CAGAGACTGC
    AGCAGGGGCA TTCGAAACAG GAAGCGTGTT TGCAACAACC CAGAGCCCAA GTATGGAGGT
    ATGCCATGCC TTGGTCCATC ACTGGAGTTC CAGGAATGCA ACATTTTACC CTGTCCAGTG
    GATGGTGTGT GGTCTTGCTG GTCATCCTGG TCTAAATGCT CAGCAACTTG TGGGGGTGGA
    CACTACATGA GAACCCGTTC GTGTACGAAT CCAGCCCCAG CATACGGAGG GGACATCTGC
    CTGGGACTGC ACACGGAAGA GGCCCTCTGT AACACACAGA CCTGCCCAGA GAACTGGTCA
    GAGTGGTCAG AGTGGTCTGT GTGTGATGCA TCTGGTACCC AGGTCCGTAC TCGGCAGTGC
    ATCCTTCTGT TTCCTGTGGG CAGCCAGTGT TCTGGAAATA CTACAGAGAG CCGGCCTTGT
    GTATTTGACT CTAATTTCAT CCCAGAAGTG TCTGTGGCAA GATCCAGCAG CGTAGAAGAG
    AAAAGGTGTG GAGAGTTCAA CATGTTCCAC ATGATGGCTG TGGGACTTAG CAGTTCCATC
    CTCGGCTGCC TCCTTACACT GCTTGTCTAC ACCTACTGCC AGCGGTACCA GCAGCAGTCC
    CATGATGCAA CTGTCATCCA TCCTGTTTCT CCCGCCGCCC TCAACAGCAG CATAACTAAC
    CACATCAACA AACTGGATAA ATACGATTCC GTGGAGGCCA TCAAGGCATT TAACAAAAAC
    AACTTGATCC TAGAGGAGAG AAACAAATAC TTCAACCCAC ATCTCACTGG GAAGACCTAC
    TCCAACGCCT ACTTTACAGA TCTCAACAAT TATGATGAAT ACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001101129.1
    CDS667..3891
    Translation

    Target ORF information:

    RefSeq Version NM_001107659.2
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus semaphorin 5A (Sema5a), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001107659.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGAAGGGAG CCTGCATCCT TGCATGGCTG TTCTCAAGCC TGGGAGTGTG GAGACTTGCT 
    CGGCCTGAGA CCCAGGACCC TGCCAAGTGC CAGAGAGCTG AGCACCCCGT CGTCTCCTAC
    AAAGAAATTG GCCCCTGGTT ACGGGAATTC AGAGCCGAGA ATGCTGTGGA TTTCTCGAGG
    TTAACATTTG ACCCAGGACA GAAAGAACTT GTCGTAGGAG CAAGAAACTA TCTCTTCAGA
    CTACAGCTCG AGGATCTGTC TCTCATCCAG GCTGTGCAGT GGGAGTGTGA TGAAGCCACG
    AAGAAGGCCT GTTACAGCAA GGGCAAATCG AAGGAGGAAT GTCAGAACTA CATACGAGTG
    CTCTTAGTGG GCGGGGACCG GCTCTTCACC TGTGGGACCA ATGCCTTCAC ACCCGTCTGT
    ACCATCCGCT CCTTAAGTAA CCTGACTGAG ATCCATGATC AGATCAGTGG CATGGCTCGA
    TGTCCCTATA GTCCCCAGCA CAATTCTACA GCTCTACTCA CTGCCAGTGG CGAGCTCTAT
    GCTGCAACAG CCATGGATTT CCCAGGGCGC GATCCAGCCA TTTATCGAAG TCTGGGCACT
    TTACCTCCTC TTCGAACTGC ACAGTACAAC TCCAAATGGC TCAATGAACC AAACTTTGTG
    TCTTCCTACG ACATCGGGAA CTTCACCTAC TTCTTCTTCC GAGAAAATGC CGTGGAACAC
    GACTGTGGGA AGACTGTGTT CTCCAGGGCA GCCAGGGTCT GCAAGAATGA CATTGGAGGA
    CGATTTCTTC TGGAGGATAC CTGGACTACC TTCATGAAGG CTCGCCTCAA CTGCTCTCGG
    CCTGGTGAGG TGCCGTTCTA CTACAACGAG CTTCAAAGCA CCTTCTTCCT GCCGGAGCTG
    GATCTGATCT ACGGCATCTT CACCACCAAT GTAAACAGCA TTGCTGCCTC TGCTGTCTGT
    GTCTTCAACC TGAGCGCCAT CTCACAGGCC TTCAATGGAC CCTTCAAGTA CCAAGAGAAC
    TCTCGCTCAG CCTGGCTGCC TTATCCCAAC CCCAACCCCA ATTTTCAGTG CGGCACCATG
    GACCAGGGCC TGTATGTAAA CCTGACGGAA AGAAACCTAC AGGATGCTCA GAAGTTCATC
    CTGATGCATG AAGTGGTGCA GCCAGTGACC ACAGTGCCTT CCTTCATGGA AGACAACAGC
    CGTTTCTCCC ATGTGGCTGT CGATGTTGTG CAAGGCAGGG ACACCCTTGT CCATATCATC
    TATCTGGCCA CAGATTATGG CACCATTAAA AAAGTGCGGG CTCCGCTGAG TCAGAGCTCG
    GGCAGCTGTT TGCTAGAAGA GATTGAGCTT TTTCCAGAGA GGAAGAGTGA GCCCATCAGG
    AGCCTAAAGA TCCTCCACAG CCAGAGTGTC CTATTTGTGG GGCTACAGGA GCATGTGGTC
    AAGATCCCCC TGAAGAGATG CCATTTTCAT CAAACACGTG GTGCCTGCAT TGGTGCTCAG
    GACCCTTACT GTGGCTGGGA TGCGGTGATG AAGAAGTGCA CCAGCCTGGA GGAGAGCCTG
    AGCATGACCC AGTGGGATCA GAGCGTCCCC ACCTGTCCGA CCAGAAATCT CACCGTAGAT
    GGGAGCTTTG GCCCGTGGTC TCCATGGACA CCCTGTACTC ACACTGATGG CACTGCTGTG
    GGATCCTGCC TCTGCCGGTC CCGATCCTGT GACAGCCCAG CTCCACAGTG TGGCGGCTGG
    CAGTGTGAGG GCCCTAGAAT GGAGATCACC AACTGTTCCA GGAATGGAGG CTGGACTCCC
    TGGACCTCCT GGTCTCCCTG CAGCACCACT TGTGGCATTG GCTTCCAGGT GCGGCAGCGC
    TCCTGCAGCA ACCCTACGCC CAGGCATGGA GGGCGTGTGT GCGTGGGCCA GAACAGGGAG
    GAAAGATACT GCAATGAACA TTTACTCTGC CCACCACACG TGTTCTGGAC AGGCTGGGGA
    CCTTGGGAAC GCTGCACAGC CCAGTGCGGG GGTGGCATTC AAGCTCGCCG TAGGACCTGT
    GAAAATGGGC CTGACTGTGC AGGATGCAAT GTGGAATACC AGCCTTGTAA CACCAATGCA
    TGCCCAGAGT TGAAGAAGAC CACACCATGG ACCCCCTGGA CACCTGTCAA CATCTCTGAC
    AATGGGGGCC ACTATGAGCA GCGGTTCCGC TACACCTGTA AAGCTCGCCT GCCAGATCCA
    AACTTATTGG AAGTAGGAAG ACAAAGGATA GAAATGCGGT ACTGTTCTAG TGATGGGACC
    AGTGGCTGCT CCACAGATGG ACTTTCTGGA GACTTTCTAA GAGCTGGGAG ATACTCTGCC
    CATACAGTCA ATGGGGCATG GTCAGCCTGG ACTTCCTGGT CACAGTGCAG CAGAGACTGC
    AGCAGGGGCA TTCGAAACAG GAAGCGTGTT TGCAACAACC CAGAGCCCAA GTATGGAGGT
    ATGCCATGCC TTGGTCCATC ACTGGAGTTC CAGGAATGCA ACATTTTACC CTGTCCAGTG
    GATGGTGTGT GGTCTTGCTG GTCATCCTGG TCTAAATGCT CAGCAACTTG TGGGGGTGGA
    CACTACATGA GAACCCGTTC GTGTACGAAT CCAGCCCCAG CATACGGAGG GGACATCTGC
    CTGGGACTGC ACACGGAAGA GGCCCTCTGT AACACACAGA CCTGCCCAGA GAACTGGTCA
    GAGTGGTCAG AGTGGTCTGT GTGTGATGCA TCTGGTACCC AGGTCCGTAC TCGGCAGTGC
    ATCCTTCTGT TTCCTGTGGG CAGCCAGTGT TCTGGAAATA CTACAGAGAG CCGGCCTTGT
    GTATTTGACT CTAATTTCAT CCCAGAAGTG TCTGTGGCAA GATCCAGCAG CGTAGAAGAG
    AAAAGGTGTG GAGAGTTCAA CATGTTCCAC ATGATGGCTG TGGGACTTAG CAGTTCCATC
    CTCGGCTGCC TCCTTACACT GCTTGTCTAC ACCTACTGCC AGCGGTACCA GCAGCAGTCC
    CATGATGCAA CTGTCATCCA TCCTGTTTCT CCCGCCGCCC TCAACAGCAG CATAACTAAC
    CACATCAACA AACTGGATAA ATACGATTCC GTGGAGGCCA TCAAGGCATT TAACAAAAAC
    AACTTGATCC TAGAGGAGAG AAACAAATAC TTCAACCCAC ATCTCACTGG GAAGACCTAC
    TCCAACGCCT ACTTTACAGA TCTCAACAAT TATGATGAAT ACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ORa05493
    Clone ID Related Accession (Same CDS sequence) XM_017590833.1 , NM_001107659.2
    Accession Version XM_017590833.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3225bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-07-25
    Organism Rattus norvegicus(Norway rat)
    Product semaphorin-5A isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005101.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGAAGGGAG CCTGCATCCT TGCATGGCTG TTCTCAAGCC TGGGAGTGTG GAGACTTGCT 
    CGGCCTGAGA CCCAGGACCC TGCCAAGTGC CAGAGAGCTG AGCACCCCGT CGTCTCCTAC
    AAAGAAATTG GCCCCTGGTT ACGGGAATTC AGAGCCGAGA ATGCTGTGGA TTTCTCGAGG
    TTAACATTTG ACCCAGGACA GAAAGAACTT GTCGTAGGAG CAAGAAACTA TCTCTTCAGA
    CTACAGCTCG AGGATCTGTC TCTCATCCAG GCTGTGCAGT GGGAGTGTGA TGAAGCCACG
    AAGAAGGCCT GTTACAGCAA GGGCAAATCG AAGGAGGAAT GTCAGAACTA CATACGAGTG
    CTCTTAGTGG GCGGGGACCG GCTCTTCACC TGTGGGACCA ATGCCTTCAC ACCCGTCTGT
    ACCATCCGCT CCTTAAGTAA CCTGACTGAG ATCCATGATC AGATCAGTGG CATGGCTCGA
    TGTCCCTATA GTCCCCAGCA CAATTCTACA GCTCTACTCA CTGCCAGTGG CGAGCTCTAT
    GCTGCAACAG CCATGGATTT CCCAGGGCGC GATCCAGCCA TTTATCGAAG TCTGGGCACT
    TTACCTCCTC TTCGAACTGC ACAGTACAAC TCCAAATGGC TCAATGAACC AAACTTTGTG
    TCTTCCTACG ACATCGGGAA CTTCACCTAC TTCTTCTTCC GAGAAAATGC CGTGGAACAC
    GACTGTGGGA AGACTGTGTT CTCCAGGGCA GCCAGGGTCT GCAAGAATGA CATTGGAGGA
    CGATTTCTTC TGGAGGATAC CTGGACTACC TTCATGAAGG CTCGCCTCAA CTGCTCTCGG
    CCTGGTGAGG TGCCGTTCTA CTACAACGAG CTTCAAAGCA CCTTCTTCCT GCCGGAGCTG
    GATCTGATCT ACGGCATCTT CACCACCAAT GTAAACAGCA TTGCTGCCTC TGCTGTCTGT
    GTCTTCAACC TGAGCGCCAT CTCACAGGCC TTCAATGGAC CCTTCAAGTA CCAAGAGAAC
    TCTCGCTCAG CCTGGCTGCC TTATCCCAAC CCCAACCCCA ATTTTCAGTG CGGCACCATG
    GACCAGGGCC TGTATGTAAA CCTGACGGAA AGAAACCTAC AGGATGCTCA GAAGTTCATC
    CTGATGCATG AAGTGGTGCA GCCAGTGACC ACAGTGCCTT CCTTCATGGA AGACAACAGC
    CGTTTCTCCC ATGTGGCTGT CGATGTTGTG CAAGGCAGGG ACACCCTTGT CCATATCATC
    TATCTGGCCA CAGATTATGG CACCATTAAA AAAGTGCGGG CTCCGCTGAG TCAGAGCTCG
    GGCAGCTGTT TGCTAGAAGA GATTGAGCTT TTTCCAGAGA GGAAGAGTGA GCCCATCAGG
    AGCCTAAAGA TCCTCCACAG CCAGAGTGTC CTATTTGTGG GGCTACAGGA GCATGTGGTC
    AAGATCCCCC TGAAGAGATG CCATTTTCAT CAAACACGTG GTGCCTGCAT TGGTGCTCAG
    GACCCTTACT GTGGCTGGGA TGCGGTGATG AAGAAGTGCA CCAGCCTGGA GGAGAGCCTG
    AGCATGACCC AGTGGGATCA GAGCGTCCCC ACCTGTCCGA CCAGAAATCT CACCGTAGAT
    GGGAGCTTTG GCCCGTGGTC TCCATGGACA CCCTGTACTC ACACTGATGG CACTGCTGTG
    GGATCCTGCC TCTGCCGGTC CCGATCCTGT GACAGCCCAG CTCCACAGTG TGGCGGCTGG
    CAGTGTGAGG GCCCTAGAAT GGAGATCACC AACTGTTCCA GGAATGGAGG CTGGACTCCC
    TGGACCTCCT GGTCTCCCTG CAGCACCACT TGTGGCATTG GCTTCCAGGT GCGGCAGCGC
    TCCTGCAGCA ACCCTACGCC CAGGCATGGA GGGCGTGTGT GCGTGGGCCA GAACAGGGAG
    GAAAGATACT GCAATGAACA TTTACTCTGC CCACCACACG TGTTCTGGAC AGGCTGGGGA
    CCTTGGGAAC GCTGCACAGC CCAGTGCGGG GGTGGCATTC AAGCTCGCCG TAGGACCTGT
    GAAAATGGGC CTGACTGTGC AGGATGCAAT GTGGAATACC AGCCTTGTAA CACCAATGCA
    TGCCCAGAGT TGAAGAAGAC CACACCATGG ACCCCCTGGA CACCTGTCAA CATCTCTGAC
    AATGGGGGCC ACTATGAGCA GCGGTTCCGC TACACCTGTA AAGCTCGCCT GCCAGATCCA
    AACTTATTGG AAGTAGGAAG ACAAAGGATA GAAATGCGGT ACTGTTCTAG TGATGGGACC
    AGTGGCTGCT CCACAGATGG ACTTTCTGGA GACTTTCTAA GAGCTGGGAG ATACTCTGCC
    CATACAGTCA ATGGGGCATG GTCAGCCTGG ACTTCCTGGT CACAGTGCAG CAGAGACTGC
    AGCAGGGGCA TTCGAAACAG GAAGCGTGTT TGCAACAACC CAGAGCCCAA GTATGGAGGT
    ATGCCATGCC TTGGTCCATC ACTGGAGTTC CAGGAATGCA ACATTTTACC CTGTCCAGTG
    GATGGTGTGT GGTCTTGCTG GTCATCCTGG TCTAAATGCT CAGCAACTTG TGGGGGTGGA
    CACTACATGA GAACCCGTTC GTGTACGAAT CCAGCCCCAG CATACGGAGG GGACATCTGC
    CTGGGACTGC ACACGGAAGA GGCCCTCTGT AACACACAGA CCTGCCCAGA GAACTGGTCA
    GAGTGGTCAG AGTGGTCTGT GTGTGATGCA TCTGGTACCC AGGTCCGTAC TCGGCAGTGC
    ATCCTTCTGT TTCCTGTGGG CAGCCAGTGT TCTGGAAATA CTACAGAGAG CCGGCCTTGT
    GTATTTGACT CTAATTTCAT CCCAGAAGTG TCTGTGGCAA GATCCAGCAG CGTAGAAGAG
    AAAAGGTGTG GAGAGTTCAA CATGTTCCAC ATGATGGCTG TGGGACTTAG CAGTTCCATC
    CTCGGCTGCC TCCTTACACT GCTTGTCTAC ACCTACTGCC AGCGGTACCA GCAGCAGTCC
    CATGATGCAA CTGTCATCCA TCCTGTTTCT CCCGCCGCCC TCAACAGCAG CATAACTAAC
    CACATCAACA AACTGGATAA ATACGATTCC GTGGAGGCCA TCAAGGCATT TAACAAAAAC
    AACTTGATCC TAGAGGAGAG AAACAAATAC TTCAACCCAC ATCTCACTGG GAAGACCTAC
    TCCAACGCCT ACTTTACAGA TCTCAACAAT TATGATGAAT ACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017446322.1
    CDS3749..6973
    Translation

    Target ORF information:

    RefSeq Version XM_017590833.1
    Organism Rattus norvegicus(Norway rat)
    Definition Rattus norvegicus semaphorin 5A (Sema5a), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017590833.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGAAGGGAG CCTGCATCCT TGCATGGCTG TTCTCAAGCC TGGGAGTGTG GAGACTTGCT 
    CGGCCTGAGA CCCAGGACCC TGCCAAGTGC CAGAGAGCTG AGCACCCCGT CGTCTCCTAC
    AAAGAAATTG GCCCCTGGTT ACGGGAATTC AGAGCCGAGA ATGCTGTGGA TTTCTCGAGG
    TTAACATTTG ACCCAGGACA GAAAGAACTT GTCGTAGGAG CAAGAAACTA TCTCTTCAGA
    CTACAGCTCG AGGATCTGTC TCTCATCCAG GCTGTGCAGT GGGAGTGTGA TGAAGCCACG
    AAGAAGGCCT GTTACAGCAA GGGCAAATCG AAGGAGGAAT GTCAGAACTA CATACGAGTG
    CTCTTAGTGG GCGGGGACCG GCTCTTCACC TGTGGGACCA ATGCCTTCAC ACCCGTCTGT
    ACCATCCGCT CCTTAAGTAA CCTGACTGAG ATCCATGATC AGATCAGTGG CATGGCTCGA
    TGTCCCTATA GTCCCCAGCA CAATTCTACA GCTCTACTCA CTGCCAGTGG CGAGCTCTAT
    GCTGCAACAG CCATGGATTT CCCAGGGCGC GATCCAGCCA TTTATCGAAG TCTGGGCACT
    TTACCTCCTC TTCGAACTGC ACAGTACAAC TCCAAATGGC TCAATGAACC AAACTTTGTG
    TCTTCCTACG ACATCGGGAA CTTCACCTAC TTCTTCTTCC GAGAAAATGC CGTGGAACAC
    GACTGTGGGA AGACTGTGTT CTCCAGGGCA GCCAGGGTCT GCAAGAATGA CATTGGAGGA
    CGATTTCTTC TGGAGGATAC CTGGACTACC TTCATGAAGG CTCGCCTCAA CTGCTCTCGG
    CCTGGTGAGG TGCCGTTCTA CTACAACGAG CTTCAAAGCA CCTTCTTCCT GCCGGAGCTG
    GATCTGATCT ACGGCATCTT CACCACCAAT GTAAACAGCA TTGCTGCCTC TGCTGTCTGT
    GTCTTCAACC TGAGCGCCAT CTCACAGGCC TTCAATGGAC CCTTCAAGTA CCAAGAGAAC
    TCTCGCTCAG CCTGGCTGCC TTATCCCAAC CCCAACCCCA ATTTTCAGTG CGGCACCATG
    GACCAGGGCC TGTATGTAAA CCTGACGGAA AGAAACCTAC AGGATGCTCA GAAGTTCATC
    CTGATGCATG AAGTGGTGCA GCCAGTGACC ACAGTGCCTT CCTTCATGGA AGACAACAGC
    CGTTTCTCCC ATGTGGCTGT CGATGTTGTG CAAGGCAGGG ACACCCTTGT CCATATCATC
    TATCTGGCCA CAGATTATGG CACCATTAAA AAAGTGCGGG CTCCGCTGAG TCAGAGCTCG
    GGCAGCTGTT TGCTAGAAGA GATTGAGCTT TTTCCAGAGA GGAAGAGTGA GCCCATCAGG
    AGCCTAAAGA TCCTCCACAG CCAGAGTGTC CTATTTGTGG GGCTACAGGA GCATGTGGTC
    AAGATCCCCC TGAAGAGATG CCATTTTCAT CAAACACGTG GTGCCTGCAT TGGTGCTCAG
    GACCCTTACT GTGGCTGGGA TGCGGTGATG AAGAAGTGCA CCAGCCTGGA GGAGAGCCTG
    AGCATGACCC AGTGGGATCA GAGCGTCCCC ACCTGTCCGA CCAGAAATCT CACCGTAGAT
    GGGAGCTTTG GCCCGTGGTC TCCATGGACA CCCTGTACTC ACACTGATGG CACTGCTGTG
    GGATCCTGCC TCTGCCGGTC CCGATCCTGT GACAGCCCAG CTCCACAGTG TGGCGGCTGG
    CAGTGTGAGG GCCCTAGAAT GGAGATCACC AACTGTTCCA GGAATGGAGG CTGGACTCCC
    TGGACCTCCT GGTCTCCCTG CAGCACCACT TGTGGCATTG GCTTCCAGGT GCGGCAGCGC
    TCCTGCAGCA ACCCTACGCC CAGGCATGGA GGGCGTGTGT GCGTGGGCCA GAACAGGGAG
    GAAAGATACT GCAATGAACA TTTACTCTGC CCACCACACG TGTTCTGGAC AGGCTGGGGA
    CCTTGGGAAC GCTGCACAGC CCAGTGCGGG GGTGGCATTC AAGCTCGCCG TAGGACCTGT
    GAAAATGGGC CTGACTGTGC AGGATGCAAT GTGGAATACC AGCCTTGTAA CACCAATGCA
    TGCCCAGAGT TGAAGAAGAC CACACCATGG ACCCCCTGGA CACCTGTCAA CATCTCTGAC
    AATGGGGGCC ACTATGAGCA GCGGTTCCGC TACACCTGTA AAGCTCGCCT GCCAGATCCA
    AACTTATTGG AAGTAGGAAG ACAAAGGATA GAAATGCGGT ACTGTTCTAG TGATGGGACC
    AGTGGCTGCT CCACAGATGG ACTTTCTGGA GACTTTCTAA GAGCTGGGAG ATACTCTGCC
    CATACAGTCA ATGGGGCATG GTCAGCCTGG ACTTCCTGGT CACAGTGCAG CAGAGACTGC
    AGCAGGGGCA TTCGAAACAG GAAGCGTGTT TGCAACAACC CAGAGCCCAA GTATGGAGGT
    ATGCCATGCC TTGGTCCATC ACTGGAGTTC CAGGAATGCA ACATTTTACC CTGTCCAGTG
    GATGGTGTGT GGTCTTGCTG GTCATCCTGG TCTAAATGCT CAGCAACTTG TGGGGGTGGA
    CACTACATGA GAACCCGTTC GTGTACGAAT CCAGCCCCAG CATACGGAGG GGACATCTGC
    CTGGGACTGC ACACGGAAGA GGCCCTCTGT AACACACAGA CCTGCCCAGA GAACTGGTCA
    GAGTGGTCAG AGTGGTCTGT GTGTGATGCA TCTGGTACCC AGGTCCGTAC TCGGCAGTGC
    ATCCTTCTGT TTCCTGTGGG CAGCCAGTGT TCTGGAAATA CTACAGAGAG CCGGCCTTGT
    GTATTTGACT CTAATTTCAT CCCAGAAGTG TCTGTGGCAA GATCCAGCAG CGTAGAAGAG
    AAAAGGTGTG GAGAGTTCAA CATGTTCCAC ATGATGGCTG TGGGACTTAG CAGTTCCATC
    CTCGGCTGCC TCCTTACACT GCTTGTCTAC ACCTACTGCC AGCGGTACCA GCAGCAGTCC
    CATGATGCAA CTGTCATCCA TCCTGTTTCT CCCGCCGCCC TCAACAGCAG CATAACTAAC
    CACATCAACA AACTGGATAA ATACGATTCC GTGGAGGCCA TCAAGGCATT TAACAAAAAC
    AACTTGATCC TAGAGGAGAG AAACAAATAC TTCAACCCAC ATCTCACTGG GAAGACCTAC
    TCCAACGCCT ACTTTACAGA TCTCAACAAT TATGATGAAT ACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.