Pde8b cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Pde8b
Entrez Gene ID	309962
Full Name	phosphodiesterase 8B
Synonyms	RNPDE8B
General protein information	Preferred Names phosphodiesterase 8B Names high affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8B cAMP-specific cyclic nucleotide phosphodiesterase PDE8
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 2 Map Location: 2q12
Summary	a cAMP-specific member of the PDE superfamily of cyclic nucleotide phosphodiesterases; involved in inhibition of intracellular cAMP signalling [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_008760670.2	XP_008758892.1	high affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8B isoform X1
XM_017590816.1	XP_017446305.1	high affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8B isoform X2
XM_017590817.1	XP_017446306.1	high affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8B isoform X3
XM_006231811.3	XP_006231873.1	high affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8B isoform X4
XM_017590818.1	XP_017446307.1	high affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8B isoform X5
XM_017590819.1	XP_017446308.1	high affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8B isoform X6
NM_199268.1	NP_954889.1	high affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8B

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	PDE8B	XP_546056.4
Bos taurus (cattle)	PDE8B	XP_003586548.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007163	XP_308604.4
Gallus gallus (chicken)	PDE8B	XP_425218.4
Danio rerio (zebrafish)	pde8b	XP_005170307.1
Drosophila melanogaster (fruit fly)	Pde8	NP_001163274.1
Xenopus tropicalis (tropical clawed frog)	pde8b	XP_002940920.1
Pan troglodytes (chimpanzee)	PDE8B	XP_517695.4
Mus musculus (house mouse)	Pde8b	NP_758467.2
Rattus norvegicus (Norway rat)	Pde8b	NP_954889.1
Homo sapiens (human)	PDE8B	NP_003710.1

Gene Ontology
Bibliography

Related articles in PubMed

Inactivation of Pde8b enhances memory, motor performance, and protects against age-induced motor coordination decay.
Tsai LC, Chan GC, Nangle SN, Shimizu-Albergine M, Jones GL, Storm DR, Beavo JA, Zweifel LS
Genes, brain, and behavior11(7)837-47(2012 Oct)

The high-affinity cAMP-specific phosphodiesterase 8B controls steroidogenesis in the mouse adrenal gland.
Tsai LC, Shimizu-Albergine M, Beavo JA
Molecular pharmacology79(4)639-48(2011 Apr)

Phosphodiesterase 3 and 4 comprise the major cAMP metabolizing enzymes responsible for insulin secretion in INS-1 (832/13) cells and rat islets.
Waddleton D, Wu W, Feng Y, Thompson C, Wu M, Zhou YP, Howard A, Thornberry N, Li J, Mancini JA
Biochemical pharmacology76(7)884-93(2008 Oct)

Diminished phosphodiesterase-8B potentiates biphasic insulin response to glucose.
Dov A, Abramovitch E, Warwar N, Nesher R
Endocrinology149(2)741-8(2008 Feb)

Biochemistry and physiology of cyclic nucleotide phosphodiesterases: essential components in cyclic nucleotide signaling.
Conti M, Beavo J
Annual review of biochemistry76481-511(2007)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

PDE8B, an 3-isobutyl-1-methylxanthine-insensitive cAMP-specific phosphodiesterase, could prove a novel target for enhanced insulin response
Title: Diminished phosphodiesterase-8B potentiates biphasic insulin response to glucose.

The following Pde8b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Pde8b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa05227	NM_199268.1 Latest version!	Rattus norvegicus phosphodiesterase 8B (Pde8b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
ORa59712	XM_017590818.1 Latest version!	Rattus norvegicus phosphodiesterase 8B (Pde8b), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
ORa59713	XM_017590819.1 Latest version!	Rattus norvegicus phosphodiesterase 8B (Pde8b), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
ORa24328	XM_006231811.3 Latest version!	Rattus norvegicus phosphodiesterase 8B (Pde8b), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$391.30 $559.00
ORa46813	XM_008760670.2 Latest version!	Rattus norvegicus phosphodiesterase 8B (Pde8b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$475.30 $679.00
ORa59710	XM_017590816.1 Latest version!	Rattus norvegicus phosphodiesterase 8B (Pde8b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$475.30 $679.00
ORa59711	XM_017590817.1 Latest version!	Rattus norvegicus phosphodiesterase 8B (Pde8b), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$475.30 $679.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Pde8b ( NM_199268.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_954889.1 Rattus norvegicus phosphodiesterase 8B (Pde8b), mRNA.

Price & Availability↑

CloneID	ORa05227
Clone ID Related Accession (Same CDS sequence)	NM_199268.1
Accession Version	NM_199268.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2286bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-18
Organism	Rattus norvegicus(Norway rat)
Product	high affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8B
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AB092697.1. On or before Jan 3, 2004 this sequence version replaced XM_226661.2, XM_342176.1. ##Evidence-Data-START## Transcript exon combination :: AB092697.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGAGACTGA CACAGGACCC TATCCAGGTC CTGCTGATCT TTGCAAAAGA AGATAGCCAG 
AGCGATGGCT TCTGGTGGGC TTGTGACAGA GCTGGCTACA GATGCAACAT TGCTCGAACT 
CCAGAGTCGG CCCTCGAATG CTTTCTGGAT AAGCATCATG AGATTATAGT GATCGATCAC 
AGGCAGACCC GTAACTTTGA TGCAGAAGCC GTGTGCAGGT CAATCCGGGC CACGAACCCC 
TCTGAGCACA CAGTGATCCT GGCTGTGGTT TCACAAGCAT CTGATGACCA TGAAGAGGCC 
TCGGTCCTTC CTCTTCTCCA TGCAGGCTTT AACCGGAGAT TTATGGAGAA CAGCAGCATA 
ATTGCTTGCT ATAATGAACT GATTCAAATA GAACACGGGG AAGTTCGCTC GCAGTTCAAA 
TTACGGGCCT GTAACTCAGT GTTTACAGCG TTAGACCACT GTCACGAAGC CATAGAAATA 
ACAAGTGATG ACCACGTGAT TCAGTATGTC AACCCTGCCT TTGAGAGGAT GATGGGCTAC 
CACAAAGGGG AGCTCCTGGG GAAGGAACTC GCTGACCTAC CTAAGAGTGA CAAGAACCGG 
GCAGACCTCC TGGACACCAT CAACACGTGC ATCAAGAAGG GAAAGGAGTG GCAGGGGGTT 
TACTATGCCA GACGGAAATC CGGGGACAGC ATCCAACAGC ACGTGAAGAT CACCCCAGTG 
ATTGGCCAAG GAGGGAAAAT TAGGCACTTT GTCTCCCTCA AGAAGCTGTG CTGTACCACT 
GACAGTAGCA AGCAGATCCA CAGGATTCAT CGCGATTCCG GAGACAACTC TCAGACAGAG 
CCTCATTCGT TCAGACACAA GAGCAGGAGA AAAGAGTCCA TTGATGTGAA ATCAATATCT 
TCTCGAGGCA GTGATGCGCC AAGCCTACAG AATCGCCGCT ACCCCTCCAT GGCAAGGATC 
CACTCCATGA CCATTGAGGC CCCCATCACA AAGGTTATAA ATATAATCAA CGCCGCTCAA 
GAAAACAGCC CAGTTACAGT GGCAGAAGCC TTGGACAGAG TTCTAGAAAT TTTACGGACC 
ACAGAACTGT ACTCCCCGCA GCTGGGAACC AAGGACGAAG ATCCTCACAC CAGTGACCTT 
GTCGGAGGCC TGATGACCGA TGGCTTGAGG AGACTGTCAG GGAATGAGTA CGTGTTTACA 
AAGAATGTGC ACCACAGTCA CAGTCACCTC TCGATGCCCA TCACCATCAA CGATGTCCCC 
CCAAGTATTG CCCAGCTCCT TGATAATGAG GAAAGTTGGG ACTTCAACAT CTTTGAATTG 
GAAGCTATTA CACATAAAAG GCCACTTGTG TACCTGGGCT TAAAGGTCTT CTCTCGGTTT 
GGAGTATGTG AATTTTTAAA CTGTACCGAA ACCACTCTTC GGGCCTGGCT GCAAGTGATT 
GAAGCAAACT ACCACTCCTC CAATGCCTAC CACAACTCCA CCCATGCCGC CGACGTACTG 
CACGCCACGG CTTTCTTCCT TGGAAAGGAA AGAGTGAAGG GAAGCCTGGA CCAGCTGGAT 
GAGGTGGCGG CTCTGATTGC AGCCACGGTG CATGACGTGG ATCACCCCGG AAGAACCAAC 
TCCTTCCTGT GTAACGCAGG CAGCGAGCTC GCTGTGCTCT ACAACGACAC TGCGGTCTTG 
GAGAGTCACC ACACAGCCCT GGCCTTCCAG CTCACCGTCA AGGACACCAA GTGCAACATT 
TTCAAGAATA TCGACAGGAA CCACTATCGA ACGCTGCGCC AGGCTATTAT TGACATGGTT 
TTGGCCACAG AGATGACAAA ACACTTTGAA CATGTGAACA AGTTTGTGAA CAGCATCAAC 
AAGCCTCTAG CAGCCGAGAG TGAGGGCAGC GACTGTGAAT GCAACCCTAC TGGGAAGAAC 
TTTCCCGAAA ACCAAATCCT CATCAAGCGT ATGATGATTA AGTGTGCTGA CGTGGCCAAC 
CCGTGCCGCC CCTTGGACCT GTGCATCGAA TGGGCTGGAA GGATCTCCGA GGAGTACTTT 
GCACAGACTG ACGAAGAGAA GAGGCAGGGG CTGCCTGTAG TGATGCCAGT GTTCGACCGC 
AATACCTGCA GCATCCCCAA GTCGCAGATA TCCTTCATCG ACTACTTCAT AACAGACATG 
TTTGATGCTT GGGATGCCTT TGCACACCTG CCAGCCCTGA TGCAACACCT GGCCGACAAC 
TATAAACACT GGAAGACACT GGATGACCTC AAGTGCAAAA GTCTGAGACT TCCATCTAAC 
AGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_954889.1
CDS	42..2327
Translation	MRLTQDPIQVLLIFAKEDSQSDGFWWACDRAGYRCNIARTPESALECFLDKHHEIIVIDHRQTRNFDAEAVCRSIRATNPSEHTVILAVVSQASDDHEEASVLPLLHAGFNRRFMENSSIIACYNELIQIEHGEVRSQFKLRACNSVFTALDHCHEAIEITSDDHVIQYVNPAFERMMGYHKGELLGKELADLPKSDKNRADLLDTINTCIKKGKEWQGVYYARRKSGDSIQQHVKITPVIGQGGKIRHFVSLKKLCCTTDSSKQIHRIHRDSGDNSQTEPHSFRHKSRRKESIDVKSISSRGSDAPSLQNRRYPSMARIHSMTIEAPITKVINIINAAQENSPVTVAEALDRVLEILRTTELYSPQLGTKDEDPHTSDLVGGLMTDGLRRLSGNEYVFTKNVHHSHSHLSMPITINDVPPSIAQLLDNEESWDFNIFELEAITHKRPLVYLGLKVFSRFGVCEFLNCTETTLRAWLQVIEANYHSSNAYHNSTHAADVLHATAFFLGKERVKGSLDQLDEVAALIAATVHDVDHPGRTNSFLCNAGSELAVLYNDTAVLESHHTALAFQLTVKDTKCNIFKNIDRNHYRTLRQAIIDMVLATEMTKHFEHVNKFVNSINKPLAAESEGSDCECNPTGKNFPENQILIKRMMIKCADVANPCRPLDLCIEWAGRISEEYFAQTDEEKRQGLPVVMPVFDRNTCSIPKSQISFIDYFITDMFDAWDAFAHLPALMQHLADNYKHWKTLDDLKCKSLRLPSNS

Target ORF information:

RefSeq Version	NM_199268.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus phosphodiesterase 8B (Pde8b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_199268.1

ORF Insert Sequence:

ATGAGACTGA CACAGGACCC TATCCAGGTC CTGCTGATCT TTGCAAAAGA AGATAGCCAG 
AGCGATGGCT TCTGGTGGGC TTGTGACAGA GCTGGCTACA GATGCAACAT TGCTCGAACT 
CCAGAGTCGG CCCTCGAATG CTTTCTGGAT AAGCATCATG AGATTATAGT GATCGATCAC 
AGGCAGACCC GTAACTTTGA TGCAGAAGCC GTGTGCAGGT CAATCCGGGC CACGAACCCC 
TCTGAGCACA CAGTGATCCT GGCTGTGGTT TCACAAGCAT CTGATGACCA TGAAGAGGCC 
TCGGTCCTTC CTCTTCTCCA TGCAGGCTTT AACCGGAGAT TTATGGAGAA CAGCAGCATA 
ATTGCTTGCT ATAATGAACT GATTCAAATA GAACACGGGG AAGTTCGCTC GCAGTTCAAA 
TTACGGGCCT GTAACTCAGT GTTTACAGCG TTAGACCACT GTCACGAAGC CATAGAAATA 
ACAAGTGATG ACCACGTGAT TCAGTATGTC AACCCTGCCT TTGAGAGGAT GATGGGCTAC 
CACAAAGGGG AGCTCCTGGG GAAGGAACTC GCTGACCTAC CTAAGAGTGA CAAGAACCGG 
GCAGACCTCC TGGACACCAT CAACACGTGC ATCAAGAAGG GAAAGGAGTG GCAGGGGGTT 
TACTATGCCA GACGGAAATC CGGGGACAGC ATCCAACAGC ACGTGAAGAT CACCCCAGTG 
ATTGGCCAAG GAGGGAAAAT TAGGCACTTT GTCTCCCTCA AGAAGCTGTG CTGTACCACT 
GACAGTAGCA AGCAGATCCA CAGGATTCAT CGCGATTCCG GAGACAACTC TCAGACAGAG 
CCTCATTCGT TCAGACACAA GAGCAGGAGA AAAGAGTCCA TTGATGTGAA ATCAATATCT 
TCTCGAGGCA GTGATGCGCC AAGCCTACAG AATCGCCGCT ACCCCTCCAT GGCAAGGATC 
CACTCCATGA CCATTGAGGC CCCCATCACA AAGGTTATAA ATATAATCAA CGCCGCTCAA 
GAAAACAGCC CAGTTACAGT GGCAGAAGCC TTGGACAGAG TTCTAGAAAT TTTACGGACC 
ACAGAACTGT ACTCCCCGCA GCTGGGAACC AAGGACGAAG ATCCTCACAC CAGTGACCTT 
GTCGGAGGCC TGATGACCGA TGGCTTGAGG AGACTGTCAG GGAATGAGTA CGTGTTTACA 
AAGAATGTGC ACCACAGTCA CAGTCACCTC TCGATGCCCA TCACCATCAA CGATGTCCCC 
CCAAGTATTG CCCAGCTCCT TGATAATGAG GAAAGTTGGG ACTTCAACAT CTTTGAATTG 
GAAGCTATTA CACATAAAAG GCCACTTGTG TACCTGGGCT TAAAGGTCTT CTCTCGGTTT 
GGAGTATGTG AATTTTTAAA CTGTACCGAA ACCACTCTTC GGGCCTGGCT GCAAGTGATT 
GAAGCAAACT ACCACTCCTC CAATGCCTAC CACAACTCCA CCCATGCCGC CGACGTACTG 
CACGCCACGG CTTTCTTCCT TGGAAAGGAA AGAGTGAAGG GAAGCCTGGA CCAGCTGGAT 
GAGGTGGCGG CTCTGATTGC AGCCACGGTG CATGACGTGG ATCACCCCGG AAGAACCAAC 
TCCTTCCTGT GTAACGCAGG CAGCGAGCTC GCTGTGCTCT ACAACGACAC TGCGGTCTTG 
GAGAGTCACC ACACAGCCCT GGCCTTCCAG CTCACCGTCA AGGACACCAA GTGCAACATT 
TTCAAGAATA TCGACAGGAA CCACTATCGA ACGCTGCGCC AGGCTATTAT TGACATGGTT 
TTGGCCACAG AGATGACAAA ACACTTTGAA CATGTGAACA AGTTTGTGAA CAGCATCAAC 
AAGCCTCTAG CAGCCGAGAG TGAGGGCAGC GACTGTGAAT GCAACCCTAC TGGGAAGAAC 
TTTCCCGAAA ACCAAATCCT CATCAAGCGT ATGATGATTA AGTGTGCTGA CGTGGCCAAC 
CCGTGCCGCC CCTTGGACCT GTGCATCGAA TGGGCTGGAA GGATCTCCGA GGAGTACTTT 
GCACAGACTG ACGAAGAGAA GAGGCAGGGG CTGCCTGTAG TGATGCCAGT GTTCGACCGC 
AATACCTGCA GCATCCCCAA GTCGCAGATA TCCTTCATCG ACTACTTCAT AACAGACATG 
TTTGATGCTT GGGATGCCTT TGCACACCTG CCAGCCCTGA TGCAACACCT GGCCGACAAC 
TATAAACACT GGAAGACACT GGATGACCTC AAGTGCAAAA GTCTGAGACT TCCATCTAAC 
AGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pde8b ( XM_017590818.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017446307.1 Rattus norvegicus phosphodiesterase 8B (Pde8b), transcript variant X5, mRNA.

Price & Availability↑

CloneID	ORa59712
Clone ID Related Accession (Same CDS sequence)	XM_017590818.1
Accession Version	XM_017590818.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2349bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	high affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8B isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005101.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCAGC AGCAGAGCGC GGCATGCCAG CAGGTGTCTT CTGCCGAGGT GCGCATTGGG 
CCCATGAGAC TGACACAGGA CCCTATCCAG GTCCTGCTGA TCTTTGCAAA AGAAGATAGC 
CAGAGCGATG GCTTCTGGTG GGCTTGTGAC AGAGCTGGCT ACAGATGCAA CATTGCTCGA 
ACTCCAGAGT CGGCCCTCGA ATGCTTTCTG GATAAGCATC ATGAGATTAT AGTGATCGAT 
CACAGGCAGA CCCGTAACTT TGATGCAGAA GCCGTGTGCA GGTCAATCCG GGCCACGAAC 
CCCTCTGAGC ACACAGTGAT CCTGGCTGTG GTTTCACAAG CATCTGATGA CCATGAAGAG 
GCCTCGGTCC TTCCTCTTCT CCATGCAGGC TTTAACCGGA GATTTATGGA GAACAGCAGC 
ATAATTGCTT GCTATAATGA ACTGATTCAA ATAGAACACG GGGAAGTTCG CTCGCAGTTC 
AAATTACGGG CCTGTAACTC AGTGTTTACA GCGTTAGACC ACTGTCACGA AGCCATAGAA 
ATAACAAGTG ATGACCACGT GATTCAGTAT GTCAACCCTG CCTTTGAGAG GATGATGGGC 
TACCACAAAG GGGAGCTCCT GGGGAAGGAA CTCGCTGACC TACCTAAGAG TGACAAGAAC 
CGGGCAGACC TCCTGGACAC CATCAACACG TGCATCAAGA AGGGAAAGGA GTGGCAGGGG 
GTTTACTATG CCAGACGGAA ATCCGGGGAC AGCATCCAAC AGCACGTGAA GATCACCCCA 
GTGATTGGCC AAGGAGGGAA AATTAGGCAC TTTGTCTCCC TCAAGAAGCT GTGCTGTACC 
ACTGACAGTA GCAAGCAGAT CCACAGGATT CATCGCGATT CCGGAGACAA CTCTCAGACA 
GAGCCTCATT CGTTCAGACA CAAGAGCAGG AGAAAAGAGT CCATTGATGT GAAATCAATA 
TCTTCTCGAG GCAGTGATGC GCCAAGCCTA CAGAATCGCC GCTACCCCTC CATGGCAAGG 
ATCCACTCCA TGACCATTGA GGCCCCCATC ACAAAGGTTA TAAATATAAT CAACGCCGCT 
CAAGAAAACA GCCCAGTTAC AGTGGCAGAA GCCTTGGACA GAGTTCTAGA AATTTTACGA 
ACCACAGAGC TGTACTCCCC GCAGCTGGGA ACCAAGGACG AAGATCCTCA CACCAGTGAC 
CTTGTCGGAG GCCTGATGAC CGATGGCTTG AGGAGACTGT CAGGGAATGA GTACGTGTTT 
ACAAAGAATG TGCACCACAG TCACAGTCAC CTCTCGATGC CCATCACCAT CAACGATGTC 
CCCCCAAGTA TTGCCCAGCT CCTTGATAAT GAGGAAAGTT GGGACTTCAA CATCTTTGAA 
TTGGAAGCTA TTACACATAA AAGGCCACTT GTGTACCTGG GCTTAAAGGT CTTCTCTCGG 
TTTGGAGTAT GTGAATTTTT AAACTGTACC GAAACCACTC TTCGGGCCTG GCTGCAAGTG 
ATTGAAGCAA ACTACCACTC CTCCAATGCC TACCACAACT CCACCCATGC CGCCGACGTA 
CTGCACGCCA CGGCTTTCTT CCTTGGAAAG GAAAGAGTGA AGGGAAGCCT GGACCAGCTG 
GATGAGGTGG CGGCTCTGAT TGCAGCCACG GTGCATGACG TGGATCACCC CGGAAGAACC 
AACTCCTTCC TGTGTAACGC AGGCAGCGAG CTCGCTGTGC TCTACAACGA CACTGCGGTC 
TTGGAGAGTC ACCACACAGC CCTGGCCTTC CAGCTCACCG TCAAGGACAC CAAGTGCAAC 
ATTTTCAAGA ATATCGACAG GAACCACTAT CGAACGCTGC GCCAGGCTAT TATTGACATG 
GTTTTGGCCA CAGAGATGAC AAAACACTTT GAACATGTGA ACAAGTTTGT GAACAGCATC 
AACAAGCCTC TAGCAGCCGA GAGTGAGGGC AGCGACTGTG AATGCAACCC TACTGGGAAG 
AACTTTCCCG AAAACCAAAT CCTCATCAAG CGTATGATGA TTAAGTGTGC TGACGTGGCC 
AACCCGTGCC GCCCCTTGGA CCTGTGCATC GAATGGGCTG GAAGGATCTC CGAGGAGTAC 
TTTGCACAGA CTGACGAAGA GAAGAGGCAG GGGCTGCCTG TAGTGATGCC AGTGTTCGAC 
CGCAATACCT GCAGCATCCC CAAGTCGCAG ATATCCTTCA TCGACTACTT CATAACAGAC 
ATGTTTGATG CTTGGGATGC CTTTGCACAC CTGCCAGCCC TGATGCAACA CCTGGCCGAC 
AACTATAAAC ACTGGAAGAC ACTGGATGAC CTCAAGTGCA AAAGTCTGAG ACTTCCATCT 
AACAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017446307.1
CDS	1824..4172
Translation	MAQQQSAACQQVSSAEVRIGPMRLTQDPIQVLLIFAKEDSQSDGFWWACDRAGYRCNIARTPESALECFLDKHHEIIVIDHRQTRNFDAEAVCRSIRATNPSEHTVILAVVSQASDDHEEASVLPLLHAGFNRRFMENSSIIACYNELIQIEHGEVRSQFKLRACNSVFTALDHCHEAIEITSDDHVIQYVNPAFERMMGYHKGELLGKELADLPKSDKNRADLLDTINTCIKKGKEWQGVYYARRKSGDSIQQHVKITPVIGQGGKIRHFVSLKKLCCTTDSSKQIHRIHRDSGDNSQTEPHSFRHKSRRKESIDVKSISSRGSDAPSLQNRRYPSMARIHSMTIEAPITKVINIINAAQENSPVTVAEALDRVLEILRTTELYSPQLGTKDEDPHTSDLVGGLMTDGLRRLSGNEYVFTKNVHHSHSHLSMPITINDVPPSIAQLLDNEESWDFNIFELEAITHKRPLVYLGLKVFSRFGVCEFLNCTETTLRAWLQVIEANYHSSNAYHNSTHAADVLHATAFFLGKERVKGSLDQLDEVAALIAATVHDVDHPGRTNSFLCNAGSELAVLYNDTAVLESHHTALAFQLTVKDTKCNIFKNIDRNHYRTLRQAIIDMVLATEMTKHFEHVNKFVNSINKPLAAESEGSDCECNPTGKNFPENQILIKRMMIKCADVANPCRPLDLCIEWAGRISEEYFAQTDEEKRQGLPVVMPVFDRNTCSIPKSQISFIDYFITDMFDAWDAFAHLPALMQHLADNYKHWKTLDDLKCKSLRLPSNS

Target ORF information:

RefSeq Version	XM_017590818.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus phosphodiesterase 8B (Pde8b), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017590818.1

ORF Insert Sequence:

ATGGCCCAGC AGCAGAGCGC GGCATGCCAG CAGGTGTCTT CTGCCGAGGT GCGCATTGGG 
CCCATGAGAC TGACACAGGA CCCTATCCAG GTCCTGCTGA TCTTTGCAAA AGAAGATAGC 
CAGAGCGATG GCTTCTGGTG GGCTTGTGAC AGAGCTGGCT ACAGATGCAA CATTGCTCGA 
ACTCCAGAGT CGGCCCTCGA ATGCTTTCTG GATAAGCATC ATGAGATTAT AGTGATCGAT 
CACAGGCAGA CCCGTAACTT TGATGCAGAA GCCGTGTGCA GGTCAATCCG GGCCACGAAC 
CCCTCTGAGC ACACAGTGAT CCTGGCTGTG GTTTCACAAG CATCTGATGA CCATGAAGAG 
GCCTCGGTCC TTCCTCTTCT CCATGCAGGC TTTAACCGGA GATTTATGGA GAACAGCAGC 
ATAATTGCTT GCTATAATGA ACTGATTCAA ATAGAACACG GGGAAGTTCG CTCGCAGTTC 
AAATTACGGG CCTGTAACTC AGTGTTTACA GCGTTAGACC ACTGTCACGA AGCCATAGAA 
ATAACAAGTG ATGACCACGT GATTCAGTAT GTCAACCCTG CCTTTGAGAG GATGATGGGC 
TACCACAAAG GGGAGCTCCT GGGGAAGGAA CTCGCTGACC TACCTAAGAG TGACAAGAAC 
CGGGCAGACC TCCTGGACAC CATCAACACG TGCATCAAGA AGGGAAAGGA GTGGCAGGGG 
GTTTACTATG CCAGACGGAA ATCCGGGGAC AGCATCCAAC AGCACGTGAA GATCACCCCA 
GTGATTGGCC AAGGAGGGAA AATTAGGCAC TTTGTCTCCC TCAAGAAGCT GTGCTGTACC 
ACTGACAGTA GCAAGCAGAT CCACAGGATT CATCGCGATT CCGGAGACAA CTCTCAGACA 
GAGCCTCATT CGTTCAGACA CAAGAGCAGG AGAAAAGAGT CCATTGATGT GAAATCAATA 
TCTTCTCGAG GCAGTGATGC GCCAAGCCTA CAGAATCGCC GCTACCCCTC CATGGCAAGG 
ATCCACTCCA TGACCATTGA GGCCCCCATC ACAAAGGTTA TAAATATAAT CAACGCCGCT 
CAAGAAAACA GCCCAGTTAC AGTGGCAGAA GCCTTGGACA GAGTTCTAGA AATTTTACGA 
ACCACAGAGC TGTACTCCCC GCAGCTGGGA ACCAAGGACG AAGATCCTCA CACCAGTGAC 
CTTGTCGGAG GCCTGATGAC CGATGGCTTG AGGAGACTGT CAGGGAATGA GTACGTGTTT 
ACAAAGAATG TGCACCACAG TCACAGTCAC CTCTCGATGC CCATCACCAT CAACGATGTC 
CCCCCAAGTA TTGCCCAGCT CCTTGATAAT GAGGAAAGTT GGGACTTCAA CATCTTTGAA 
TTGGAAGCTA TTACACATAA AAGGCCACTT GTGTACCTGG GCTTAAAGGT CTTCTCTCGG 
TTTGGAGTAT GTGAATTTTT AAACTGTACC GAAACCACTC TTCGGGCCTG GCTGCAAGTG 
ATTGAAGCAA ACTACCACTC CTCCAATGCC TACCACAACT CCACCCATGC CGCCGACGTA 
CTGCACGCCA CGGCTTTCTT CCTTGGAAAG GAAAGAGTGA AGGGAAGCCT GGACCAGCTG 
GATGAGGTGG CGGCTCTGAT TGCAGCCACG GTGCATGACG TGGATCACCC CGGAAGAACC 
AACTCCTTCC TGTGTAACGC AGGCAGCGAG CTCGCTGTGC TCTACAACGA CACTGCGGTC 
TTGGAGAGTC ACCACACAGC CCTGGCCTTC CAGCTCACCG TCAAGGACAC CAAGTGCAAC 
ATTTTCAAGA ATATCGACAG GAACCACTAT CGAACGCTGC GCCAGGCTAT TATTGACATG 
GTTTTGGCCA CAGAGATGAC AAAACACTTT GAACATGTGA ACAAGTTTGT GAACAGCATC 
AACAAGCCTC TAGCAGCCGA GAGTGAGGGC AGCGACTGTG AATGCAACCC TACTGGGAAG 
AACTTTCCCG AAAACCAAAT CCTCATCAAG CGTATGATGA TTAAGTGTGC TGACGTGGCC 
AACCCGTGCC GCCCCTTGGA CCTGTGCATC GAATGGGCTG GAAGGATCTC CGAGGAGTAC 
TTTGCACAGA CTGACGAAGA GAAGAGGCAG GGGCTGCCTG TAGTGATGCC AGTGTTCGAC 
CGCAATACCT GCAGCATCCC CAAGTCGCAG ATATCCTTCA TCGACTACTT CATAACAGAC 
ATGTTTGATG CTTGGGATGC CTTTGCACAC CTGCCAGCCC TGATGCAACA CCTGGCCGAC 
AACTATAAAC ACTGGAAGAC ACTGGATGAC CTCAAGTGCA AAAGTCTGAG ACTTCCATCT 
AACAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pde8b ( XM_017590819.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017446308.1 Rattus norvegicus phosphodiesterase 8B (Pde8b), transcript variant X6, mRNA.

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CloneID	ORa59713
Clone ID Related Accession (Same CDS sequence)	XM_017590819.1
Accession Version	XM_017590819.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2346bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	high affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8B isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005101.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCCAGC AGCAGAGCGC GGCATGCCAG GTGTCTTCTG CCGAGGTGCG CATTGGGCCC 
ATGAGACTGA CACAGGACCC TATCCAGGTC CTGCTGATCT TTGCAAAAGA AGATAGCCAG 
AGCGATGGCT TCTGGTGGGC TTGTGACAGA GCTGGCTACA GATGCAACAT TGCTCGAACT 
CCAGAGTCGG CCCTCGAATG CTTTCTGGAT AAGCATCATG AGATTATAGT GATCGATCAC 
AGGCAGACCC GTAACTTTGA TGCAGAAGCC GTGTGCAGGT CAATCCGGGC CACGAACCCC 
TCTGAGCACA CAGTGATCCT GGCTGTGGTT TCACAAGCAT CTGATGACCA TGAAGAGGCC 
TCGGTCCTTC CTCTTCTCCA TGCAGGCTTT AACCGGAGAT TTATGGAGAA CAGCAGCATA 
ATTGCTTGCT ATAATGAACT GATTCAAATA GAACACGGGG AAGTTCGCTC GCAGTTCAAA 
TTACGGGCCT GTAACTCAGT GTTTACAGCG TTAGACCACT GTCACGAAGC CATAGAAATA 
ACAAGTGATG ACCACGTGAT TCAGTATGTC AACCCTGCCT TTGAGAGGAT GATGGGCTAC 
CACAAAGGGG AGCTCCTGGG GAAGGAACTC GCTGACCTAC CTAAGAGTGA CAAGAACCGG 
GCAGACCTCC TGGACACCAT CAACACGTGC ATCAAGAAGG GAAAGGAGTG GCAGGGGGTT 
TACTATGCCA GACGGAAATC CGGGGACAGC ATCCAACAGC ACGTGAAGAT CACCCCAGTG 
ATTGGCCAAG GAGGGAAAAT TAGGCACTTT GTCTCCCTCA AGAAGCTGTG CTGTACCACT 
GACAGTAGCA AGCAGATCCA CAGGATTCAT CGCGATTCCG GAGACAACTC TCAGACAGAG 
CCTCATTCGT TCAGACACAA GAGCAGGAGA AAAGAGTCCA TTGATGTGAA ATCAATATCT 
TCTCGAGGCA GTGATGCGCC AAGCCTACAG AATCGCCGCT ACCCCTCCAT GGCAAGGATC 
CACTCCATGA CCATTGAGGC CCCCATCACA AAGGTTATAA ATATAATCAA CGCCGCTCAA 
GAAAACAGCC CAGTTACAGT GGCAGAAGCC TTGGACAGAG TTCTAGAAAT TTTACGAACC 
ACAGAGCTGT ACTCCCCGCA GCTGGGAACC AAGGACGAAG ATCCTCACAC CAGTGACCTT 
GTCGGAGGCC TGATGACCGA TGGCTTGAGG AGACTGTCAG GGAATGAGTA CGTGTTTACA 
AAGAATGTGC ACCACAGTCA CAGTCACCTC TCGATGCCCA TCACCATCAA CGATGTCCCC 
CCAAGTATTG CCCAGCTCCT TGATAATGAG GAAAGTTGGG ACTTCAACAT CTTTGAATTG 
GAAGCTATTA CACATAAAAG GCCACTTGTG TACCTGGGCT TAAAGGTCTT CTCTCGGTTT 
GGAGTATGTG AATTTTTAAA CTGTACCGAA ACCACTCTTC GGGCCTGGCT GCAAGTGATT 
GAAGCAAACT ACCACTCCTC CAATGCCTAC CACAACTCCA CCCATGCCGC CGACGTACTG 
CACGCCACGG CTTTCTTCCT TGGAAAGGAA AGAGTGAAGG GAAGCCTGGA CCAGCTGGAT 
GAGGTGGCGG CTCTGATTGC AGCCACGGTG CATGACGTGG ATCACCCCGG AAGAACCAAC 
TCCTTCCTGT GTAACGCAGG CAGCGAGCTC GCTGTGCTCT ACAACGACAC TGCGGTCTTG 
GAGAGTCACC ACACAGCCCT GGCCTTCCAG CTCACCGTCA AGGACACCAA GTGCAACATT 
TTCAAGAATA TCGACAGGAA CCACTATCGA ACGCTGCGCC AGGCTATTAT TGACATGGTT 
TTGGCCACAG AGATGACAAA ACACTTTGAA CATGTGAACA AGTTTGTGAA CAGCATCAAC 
AAGCCTCTAG CAGCCGAGAG TGAGGGCAGC GACTGTGAAT GCAACCCTAC TGGGAAGAAC 
TTTCCCGAAA ACCAAATCCT CATCAAGCGT ATGATGATTA AGTGTGCTGA CGTGGCCAAC 
CCGTGCCGCC CCTTGGACCT GTGCATCGAA TGGGCTGGAA GGATCTCCGA GGAGTACTTT 
GCACAGACTG ACGAAGAGAA GAGGCAGGGG CTGCCTGTAG TGATGCCAGT GTTCGACCGC 
AATACCTGCA GCATCCCCAA GTCGCAGATA TCCTTCATCG ACTACTTCAT AACAGACATG 
TTTGATGCTT GGGATGCCTT TGCACACCTG CCAGCCCTGA TGCAACACCT GGCCGACAAC 
TATAAACACT GGAAGACACT GGATGACCTC AAGTGCAAAA GTCTGAGACT TCCATCTAAC 
AGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017446308.1
CDS	1824..4169
Translation	MAQQQSAACQVSSAEVRIGPMRLTQDPIQVLLIFAKEDSQSDGFWWACDRAGYRCNIARTPESALECFLDKHHEIIVIDHRQTRNFDAEAVCRSIRATNPSEHTVILAVVSQASDDHEEASVLPLLHAGFNRRFMENSSIIACYNELIQIEHGEVRSQFKLRACNSVFTALDHCHEAIEITSDDHVIQYVNPAFERMMGYHKGELLGKELADLPKSDKNRADLLDTINTCIKKGKEWQGVYYARRKSGDSIQQHVKITPVIGQGGKIRHFVSLKKLCCTTDSSKQIHRIHRDSGDNSQTEPHSFRHKSRRKESIDVKSISSRGSDAPSLQNRRYPSMARIHSMTIEAPITKVINIINAAQENSPVTVAEALDRVLEILRTTELYSPQLGTKDEDPHTSDLVGGLMTDGLRRLSGNEYVFTKNVHHSHSHLSMPITINDVPPSIAQLLDNEESWDFNIFELEAITHKRPLVYLGLKVFSRFGVCEFLNCTETTLRAWLQVIEANYHSSNAYHNSTHAADVLHATAFFLGKERVKGSLDQLDEVAALIAATVHDVDHPGRTNSFLCNAGSELAVLYNDTAVLESHHTALAFQLTVKDTKCNIFKNIDRNHYRTLRQAIIDMVLATEMTKHFEHVNKFVNSINKPLAAESEGSDCECNPTGKNFPENQILIKRMMIKCADVANPCRPLDLCIEWAGRISEEYFAQTDEEKRQGLPVVMPVFDRNTCSIPKSQISFIDYFITDMFDAWDAFAHLPALMQHLADNYKHWKTLDDLKCKSLRLPSNS

Target ORF information:

RefSeq Version	XM_017590819.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus phosphodiesterase 8B (Pde8b), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017590819.1

ORF Insert Sequence:

ATGGCCCAGC AGCAGAGCGC GGCATGCCAG GTGTCTTCTG CCGAGGTGCG CATTGGGCCC 
ATGAGACTGA CACAGGACCC TATCCAGGTC CTGCTGATCT TTGCAAAAGA AGATAGCCAG 
AGCGATGGCT TCTGGTGGGC TTGTGACAGA GCTGGCTACA GATGCAACAT TGCTCGAACT 
CCAGAGTCGG CCCTCGAATG CTTTCTGGAT AAGCATCATG AGATTATAGT GATCGATCAC 
AGGCAGACCC GTAACTTTGA TGCAGAAGCC GTGTGCAGGT CAATCCGGGC CACGAACCCC 
TCTGAGCACA CAGTGATCCT GGCTGTGGTT TCACAAGCAT CTGATGACCA TGAAGAGGCC 
TCGGTCCTTC CTCTTCTCCA TGCAGGCTTT AACCGGAGAT TTATGGAGAA CAGCAGCATA 
ATTGCTTGCT ATAATGAACT GATTCAAATA GAACACGGGG AAGTTCGCTC GCAGTTCAAA 
TTACGGGCCT GTAACTCAGT GTTTACAGCG TTAGACCACT GTCACGAAGC CATAGAAATA 
ACAAGTGATG ACCACGTGAT TCAGTATGTC AACCCTGCCT TTGAGAGGAT GATGGGCTAC 
CACAAAGGGG AGCTCCTGGG GAAGGAACTC GCTGACCTAC CTAAGAGTGA CAAGAACCGG 
GCAGACCTCC TGGACACCAT CAACACGTGC ATCAAGAAGG GAAAGGAGTG GCAGGGGGTT 
TACTATGCCA GACGGAAATC CGGGGACAGC ATCCAACAGC ACGTGAAGAT CACCCCAGTG 
ATTGGCCAAG GAGGGAAAAT TAGGCACTTT GTCTCCCTCA AGAAGCTGTG CTGTACCACT 
GACAGTAGCA AGCAGATCCA CAGGATTCAT CGCGATTCCG GAGACAACTC TCAGACAGAG 
CCTCATTCGT TCAGACACAA GAGCAGGAGA AAAGAGTCCA TTGATGTGAA ATCAATATCT 
TCTCGAGGCA GTGATGCGCC AAGCCTACAG AATCGCCGCT ACCCCTCCAT GGCAAGGATC 
CACTCCATGA CCATTGAGGC CCCCATCACA AAGGTTATAA ATATAATCAA CGCCGCTCAA 
GAAAACAGCC CAGTTACAGT GGCAGAAGCC TTGGACAGAG TTCTAGAAAT TTTACGAACC 
ACAGAGCTGT ACTCCCCGCA GCTGGGAACC AAGGACGAAG ATCCTCACAC CAGTGACCTT 
GTCGGAGGCC TGATGACCGA TGGCTTGAGG AGACTGTCAG GGAATGAGTA CGTGTTTACA 
AAGAATGTGC ACCACAGTCA CAGTCACCTC TCGATGCCCA TCACCATCAA CGATGTCCCC 
CCAAGTATTG CCCAGCTCCT TGATAATGAG GAAAGTTGGG ACTTCAACAT CTTTGAATTG 
GAAGCTATTA CACATAAAAG GCCACTTGTG TACCTGGGCT TAAAGGTCTT CTCTCGGTTT 
GGAGTATGTG AATTTTTAAA CTGTACCGAA ACCACTCTTC GGGCCTGGCT GCAAGTGATT 
GAAGCAAACT ACCACTCCTC CAATGCCTAC CACAACTCCA CCCATGCCGC CGACGTACTG 
CACGCCACGG CTTTCTTCCT TGGAAAGGAA AGAGTGAAGG GAAGCCTGGA CCAGCTGGAT 
GAGGTGGCGG CTCTGATTGC AGCCACGGTG CATGACGTGG ATCACCCCGG AAGAACCAAC 
TCCTTCCTGT GTAACGCAGG CAGCGAGCTC GCTGTGCTCT ACAACGACAC TGCGGTCTTG 
GAGAGTCACC ACACAGCCCT GGCCTTCCAG CTCACCGTCA AGGACACCAA GTGCAACATT 
TTCAAGAATA TCGACAGGAA CCACTATCGA ACGCTGCGCC AGGCTATTAT TGACATGGTT 
TTGGCCACAG AGATGACAAA ACACTTTGAA CATGTGAACA AGTTTGTGAA CAGCATCAAC 
AAGCCTCTAG CAGCCGAGAG TGAGGGCAGC GACTGTGAAT GCAACCCTAC TGGGAAGAAC 
TTTCCCGAAA ACCAAATCCT CATCAAGCGT ATGATGATTA AGTGTGCTGA CGTGGCCAAC 
CCGTGCCGCC CCTTGGACCT GTGCATCGAA TGGGCTGGAA GGATCTCCGA GGAGTACTTT 
GCACAGACTG ACGAAGAGAA GAGGCAGGGG CTGCCTGTAG TGATGCCAGT GTTCGACCGC 
AATACCTGCA GCATCCCCAA GTCGCAGATA TCCTTCATCG ACTACTTCAT AACAGACATG 
TTTGATGCTT GGGATGCCTT TGCACACCTG CCAGCCCTGA TGCAACACCT GGCCGACAAC 
TATAAACACT GGAAGACACT GGATGACCTC AAGTGCAAAA GTCTGAGACT TCCATCTAAC 
AGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pde8b ( XM_006231811.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006231873.1 Rattus norvegicus phosphodiesterase 8B (Pde8b), transcript variant X4, mRNA.

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CloneID	ORa24328
Clone ID Related Accession (Same CDS sequence)	XM_006231811.3
Accession Version	XM_006231811.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2367bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	high affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8B isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005101.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006231811.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCTGCG CCCCCAGCAT CCATGTCTCG CAGAGCGGTG TGATCTACTG CCGGGACTCG 
GATGAGTCCA ACTCGCCCCG CCAGACCAGC AGCGTGTCGC AGGGCCCCAC GGCGCCCCTG 
CACGGCCTCT TCGTGCAGAC CGACGCCGCC GACGCCATGC CCCCGAGTCG CGCGGCGGGA 
CCCCCGGGCG CTGTCCGAGT CCGCAGGTCC CGGGCTGAGC TGGGCAGCGG CAGCAGCACC 
GGGTCCTCGG GGCCCGCGGC GACCACCTGC CGGGGCCGGA GGCGCCACTG CTGCAGCAGC 
GCCGAGGCCG AGACCCAGAC TAGCTACACC AGCGTCAAGC AGGTGTCTTC TGCCGAGGTG 
CGCATTGGGC CCATGAGACT GACACAGGAC CCTATCCAGG TCCTGCTGAT CTTTGCAAAA 
GAAGATAGCC AGAGCGATGG CTTCTGGTGG GCTTGTGACA GAGCTGGCTA CAGATGCAAC 
ATTGCTCGAA CTCCAGAGTC GGCCCTCGAA TGCTTTCTGG ATAAGCATCA TGAGATTATA 
GTGATCGATC ACAGGCAGAC CCGTAACTTT GATGCAGAAG CCGTGTGCAG GTCAATCCGG 
GCCACGAACC CCTCTGAGCA CACAGTGATC CTGGCTGTGG TTTCACAAGC ATCTGATGAC 
CATGAAGAGG CCTCGGTCCT TCCTCTTCTC CATGCAGGCT TTAACCGGAG ATTTATGGAG 
AACAGCAGCA TAATTGCTTG CTATAATGAA CTGATTCAAA TAGAACACGG GGAAGTTCGC 
TCGCAGTTCA AATTACGGGC CTGTAACTCA GTGTTTACAG CGTTAGACCA CTGTCACGAA 
GCCATAGAAA TAACAAGTGA TGACCACGTG ATTCAGATCC ACAGGATTCA TCGCGATTCC 
GGAGACAACT CTCAGACAGA GCCTCATTCG TTCAGACACA AGAGCAGGAG AAAAGAGTCC 
ATTGATGTGA AATCAATATC TTCTCGAGGC AGTGATGCGC CAAGCCTACA GAATCGCCGC 
TACCCCTCCA TGGCAAGGAT CCACTCCATG ACCATTGAGG CCCCCATCAC AAAGGTTATA 
AATATAATCA ACGCCGCTCA AGAAAACAGC CCAGTTACAG TGGCAGAAGC CTTGGACAGA 
GTTCTAGAAA TTTTACGAAC CACAGAGCTG TACTCCCCGC AGCTGGGAAC CAAGGACGAA 
GATCCTCACA CCAGTGACCT TGTCGGAGGC CTGATGACCG ATGGCTTGAG GAGACTGTCA 
GGGAATGAGT ACGTGTTTAC AAAGAATGTG CACCACAGTC ACAGTCACCT CTCGATGCCC 
ATCACCATCA ACGATGTCCC CCCAAGTATT GCCCAGCTCC TTGATAATGA GGAAAGTTGG 
GACTTCAACA TCTTTGAATT GGAAGCTATT ACACATAAAA GGCCACTTGT GTACCTGGGC 
TTAAAGGTCT TCTCTCGGTT TGGAGTATGT GAATTTTTAA ACTGTACCGA AACCACTCTT 
CGGGCCTGGC TGCAAGTGAT TGAAGCAAAC TACCACTCCT CCAATGCCTA CCACAACTCC 
ACCCATGCCG CCGACGTACT GCACGCCACG GCTTTCTTCC TTGGAAAGGA AAGAGTGAAG 
GGAAGCCTGG ACCAGCTGGA TGAGGTGGCG GCTCTGATTG CAGCCACGGT GCATGACGTG 
GATCACCCCG GAAGAACCAA CTCCTTCCTG TGTAACGCAG GCAGCGAGCT CGCTGTGCTC 
TACAACGACA CTGCGGTCTT GGAGAGTCAC CACACAGCCC TGGCCTTCCA GCTCACCGTC 
AAGGACACCA AGTGCAACAT TTTCAAGAAT ATCGACAGGA ACCACTATCG AACGCTGCGC 
CAGGCTATTA TTGACATGGT TTTGGCCACA GAGATGACAA AACACTTTGA ACATGTGAAC 
AAGTTTGTGA ACAGCATCAA CAAGCCTCTA GCAGCCGAGA GTGAGGGCAG CGACTGTGAA 
TGCAACCCTA CTGGGAAGAA CTTTCCCGAA AACCAAATCC TCATCAAGCG TATGATGATT 
AAGTGTGCTG ACGTGGCCAA CCCGTGCCGC CCCTTGGACC TGTGCATCGA ATGGGCTGGA 
AGGATCTCCG AGGAGTACTT TGCACAGACT GACGAAGAGA AGAGGCAGGG GCTGCCTGTA 
GTGATGCCAG TGTTCGACCG CAATACCTGC AGCATCCCCA AGTCGCAGAT ATCCTTCATC 
GACTACTTCA TAACAGACAT GTTTGATGCT TGGGATGCCT TTGCACACCT GCCAGCCCTG 
ATGCAACACC TGGCCGACAA CTATAAACAC TGGAAGACAC TGGATGACCT CAAGTGCAAA 
AGTCTGAGAC TTCCATCTAA CAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006231873.1
CDS	296..2662
Translation	MGCAPSIHVSQSGVIYCRDSDESNSPRQTSSVSQGPTAPLHGLFVQTDAADAMPPSRAAGPPGAVRVRRSRAELGSGSSTGSSGPAATTCRGRRRHCCSSAEAETQTSYTSVKQVSSAEVRIGPMRLTQDPIQVLLIFAKEDSQSDGFWWACDRAGYRCNIARTPESALECFLDKHHEIIVIDHRQTRNFDAEAVCRSIRATNPSEHTVILAVVSQASDDHEEASVLPLLHAGFNRRFMENSSIIACYNELIQIEHGEVRSQFKLRACNSVFTALDHCHEAIEITSDDHVIQIHRIHRDSGDNSQTEPHSFRHKSRRKESIDVKSISSRGSDAPSLQNRRYPSMARIHSMTIEAPITKVINIINAAQENSPVTVAEALDRVLEILRTTELYSPQLGTKDEDPHTSDLVGGLMTDGLRRLSGNEYVFTKNVHHSHSHLSMPITINDVPPSIAQLLDNEESWDFNIFELEAITHKRPLVYLGLKVFSRFGVCEFLNCTETTLRAWLQVIEANYHSSNAYHNSTHAADVLHATAFFLGKERVKGSLDQLDEVAALIAATVHDVDHPGRTNSFLCNAGSELAVLYNDTAVLESHHTALAFQLTVKDTKCNIFKNIDRNHYRTLRQAIIDMVLATEMTKHFEHVNKFVNSINKPLAAESEGSDCECNPTGKNFPENQILIKRMMIKCADVANPCRPLDLCIEWAGRISEEYFAQTDEEKRQGLPVVMPVFDRNTCSIPKSQISFIDYFITDMFDAWDAFAHLPALMQHLADNYKHWKTLDDLKCKSLRLPSNS

Target ORF information:

RefSeq Version	XM_006231811.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus phosphodiesterase 8B (Pde8b), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006231811.3

ORF Insert Sequence:

ATGGGCTGCG CCCCCAGCAT CCATGTCTCG CAGAGCGGTG TGATCTACTG CCGGGACTCG 
GATGAGTCCA ACTCGCCCCG CCAGACCAGC AGCGTGTCGC AGGGCCCCAC GGCGCCCCTG 
CACGGCCTCT TCGTGCAGAC CGACGCCGCC GACGCCATGC CCCCGAGTCG CGCGGCGGGA 
CCCCCGGGCG CTGTCCGAGT CCGCAGGTCC CGGGCTGAGC TGGGCAGCGG CAGCAGCACC 
GGGTCCTCGG GGCCCGCGGC GACCACCTGC CGGGGCCGGA GGCGCCACTG CTGCAGCAGC 
GCCGAGGCCG AGACCCAGAC TAGCTACACC AGCGTCAAGC AGGTGTCTTC TGCCGAGGTG 
CGCATTGGGC CCATGAGACT GACACAGGAC CCTATCCAGG TCCTGCTGAT CTTTGCAAAA 
GAAGATAGCC AGAGCGATGG CTTCTGGTGG GCTTGTGACA GAGCTGGCTA CAGATGCAAC 
ATTGCTCGAA CTCCAGAGTC GGCCCTCGAA TGCTTTCTGG ATAAGCATCA TGAGATTATA 
GTGATCGATC ACAGGCAGAC CCGTAACTTT GATGCAGAAG CCGTGTGCAG GTCAATCCGG 
GCCACGAACC CCTCTGAGCA CACAGTGATC CTGGCTGTGG TTTCACAAGC ATCTGATGAC 
CATGAAGAGG CCTCGGTCCT TCCTCTTCTC CATGCAGGCT TTAACCGGAG ATTTATGGAG 
AACAGCAGCA TAATTGCTTG CTATAATGAA CTGATTCAAA TAGAACACGG GGAAGTTCGC 
TCGCAGTTCA AATTACGGGC CTGTAACTCA GTGTTTACAG CGTTAGACCA CTGTCACGAA 
GCCATAGAAA TAACAAGTGA TGACCACGTG ATTCAGATCC ACAGGATTCA TCGCGATTCC 
GGAGACAACT CTCAGACAGA GCCTCATTCG TTCAGACACA AGAGCAGGAG AAAAGAGTCC 
ATTGATGTGA AATCAATATC TTCTCGAGGC AGTGATGCGC CAAGCCTACA GAATCGCCGC 
TACCCCTCCA TGGCAAGGAT CCACTCCATG ACCATTGAGG CCCCCATCAC AAAGGTTATA 
AATATAATCA ACGCCGCTCA AGAAAACAGC CCAGTTACAG TGGCAGAAGC CTTGGACAGA 
GTTCTAGAAA TTTTACGAAC CACAGAGCTG TACTCCCCGC AGCTGGGAAC CAAGGACGAA 
GATCCTCACA CCAGTGACCT TGTCGGAGGC CTGATGACCG ATGGCTTGAG GAGACTGTCA 
GGGAATGAGT ACGTGTTTAC AAAGAATGTG CACCACAGTC ACAGTCACCT CTCGATGCCC 
ATCACCATCA ACGATGTCCC CCCAAGTATT GCCCAGCTCC TTGATAATGA GGAAAGTTGG 
GACTTCAACA TCTTTGAATT GGAAGCTATT ACACATAAAA GGCCACTTGT GTACCTGGGC 
TTAAAGGTCT TCTCTCGGTT TGGAGTATGT GAATTTTTAA ACTGTACCGA AACCACTCTT 
CGGGCCTGGC TGCAAGTGAT TGAAGCAAAC TACCACTCCT CCAATGCCTA CCACAACTCC 
ACCCATGCCG CCGACGTACT GCACGCCACG GCTTTCTTCC TTGGAAAGGA AAGAGTGAAG 
GGAAGCCTGG ACCAGCTGGA TGAGGTGGCG GCTCTGATTG CAGCCACGGT GCATGACGTG 
GATCACCCCG GAAGAACCAA CTCCTTCCTG TGTAACGCAG GCAGCGAGCT CGCTGTGCTC 
TACAACGACA CTGCGGTCTT GGAGAGTCAC CACACAGCCC TGGCCTTCCA GCTCACCGTC 
AAGGACACCA AGTGCAACAT TTTCAAGAAT ATCGACAGGA ACCACTATCG AACGCTGCGC 
CAGGCTATTA TTGACATGGT TTTGGCCACA GAGATGACAA AACACTTTGA ACATGTGAAC 
AAGTTTGTGA ACAGCATCAA CAAGCCTCTA GCAGCCGAGA GTGAGGGCAG CGACTGTGAA 
TGCAACCCTA CTGGGAAGAA CTTTCCCGAA AACCAAATCC TCATCAAGCG TATGATGATT 
AAGTGTGCTG ACGTGGCCAA CCCGTGCCGC CCCTTGGACC TGTGCATCGA ATGGGCTGGA 
AGGATCTCCG AGGAGTACTT TGCACAGACT GACGAAGAGA AGAGGCAGGG GCTGCCTGTA 
GTGATGCCAG TGTTCGACCG CAATACCTGC AGCATCCCCA AGTCGCAGAT ATCCTTCATC 
GACTACTTCA TAACAGACAT GTTTGATGCT TGGGATGCCT TTGCACACCT GCCAGCCCTG 
ATGCAACACC TGGCCGACAA CTATAAACAC TGGAAGACAC TGGATGACCT CAAGTGCAAA 
AGTCTGAGAC TTCCATCTAA CAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pde8b ( XM_008760670.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008758892.1 Rattus norvegicus phosphodiesterase 8B (Pde8b), transcript variant X1, mRNA.

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CloneID	ORa46813
Clone ID Related Accession (Same CDS sequence)	XM_008760670.2
Accession Version	XM_008760670.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2658bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	high affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005101.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008760670.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCTGCG CCCCCAGCAT CCATGTCTCG CAGAGCGGTG TGATCTACTG CCGGGACTCG 
GATGAGTCCA ACTCGCCCCG CCAGACCAGC AGCGTGTCGC AGGGCCCCAC GGCGCCCCTG 
CACGGCCTCT TCGTGCAGAC CGACGCCGCC GACGCCATGC CCCCGAGTCG CGCGGCGGGA 
CCCCCGGGCG CTGTCCGAGT CCGCAGGTCC CGGGCTGAGC TGGGCAGCGG CAGCAGCACC 
GGGTCCTCGG GGCCCGCGGC GACCACCTGC CGGGGCCGGA GGCGCCACTG CTGCAGCAGC 
GCCGAGGCCG AGACCCAGAC TAGCTACACC AGCGTCAAGC AGGTGTCTTC TGCCGAGGTG 
CGCATTGGGC CCATGAGACT GACACAGGAC CCTATCCAGG TCCTGCTGAT CTTTGCAAAA 
GAAGATAGCC AGAGCGATGG CTTCTGGTGG GCTTGTGACA GAGCTGGCTA CAGATGCAAC 
ATTGCTCGAA CTCCAGAGTC GGCCCTCGAA TGCTTTCTGG ATAAGCATCA TGAGATTATA 
GTGATCGATC ACAGGCAGAC CCGTAACTTT GATGCAGAAG CCGTGTGCAG GTCAATCCGG 
GCCACGAACC CCTCTGAGCA CACAGTGATC CTGGCTGTGG TTTCACAAGC ATCTGATGAC 
CATGAAGAGG CCTCGGTCCT TCCTCTTCTC CATGCAGGCT TTAACCGGAG ATTTATGGAG 
AACAGCAGCA TAATTGCTTG CTATAATGAA CTGATTCAAA TAGAACACGG GGAAGTTCGC 
TCGCAGTTCA AATTACGGGC CTGTAACTCA GTGTTTACAG CGTTAGACCA CTGTCACGAA 
GCCATAGAAA TAACAAGTGA TGACCACGTG ATTCAGTATG TCAACCCTGC CTTTGAGAGG 
ATGATGGGCT ACCACAAAGG GGAGCTCCTG GGGAAGGAAC TCGCTGACCT ACCTAAGAGT 
GACAAGAACC GGGCAGACCT CCTGGACACC ATCAACACGT GCATCAAGAA GGGAAAGGAG 
TGGCAGGGGG TTTACTATGC CAGACGGAAA TCCGGGGACA GCATCCAACA GCACGTGAAG 
ATCACCCCAG TGATTGGCCA AGGAGGGAAA ATTAGGCACT TTGTCTCCCT CAAGAAGCTG 
TGCTGTACCA CTGACAGTAG CAAGCAGATC CACAGGATTC ATCGCGATTC CGGAGACAAC 
TCTCAGACAG AGCCTCATTC GTTCAGACAC AAGAGCAGGA GAAAAGAGTC CATTGATGTG 
AAATCAATAT CTTCTCGAGG CAGTGATGCG CCAAGCCTAC AGAATCGCCG CTACCCCTCC 
ATGGCAAGGA TCCACTCCAT GACCATTGAG GCCCCCATCA CAAAGGTTAT AAATATAATC 
AACGCCGCTC AAGAAAACAG CCCAGTTACA GTGGCAGAAG CCTTGGACAG AGTTCTAGAA 
ATTTTACGAA CCACAGAGCT GTACTCCCCG CAGCTGGGAA CCAAGGACGA AGATCCTCAC 
ACCAGTGACC TTGTCGGAGG CCTGATGACC GATGGCTTGA GGAGACTGTC AGGGAATGAG 
TACGTGTTTA CAAAGAATGT GCACCACAGT CACAGTCACC TCTCGATGCC CATCACCATC 
AACGATGTCC CCCCAAGTAT TGCCCAGCTC CTTGATAATG AGGAAAGTTG GGACTTCAAC 
ATCTTTGAAT TGGAAGCTAT TACACATAAA AGGCCACTTG TGTACCTGGG CTTAAAGGTC 
TTCTCTCGGT TTGGAGTATG TGAATTTTTA AACTGTACCG AAACCACTCT TCGGGCCTGG 
CTGCAAGTGA TTGAAGCAAA CTACCACTCC TCCAATGCCT ACCACAACTC CACCCATGCC 
GCCGACGTAC TGCACGCCAC GGCTTTCTTC CTTGGAAAGG AAAGAGTGAA GGGAAGCCTG 
GACCAGCTGG ATGAGGTGGC GGCTCTGATT GCAGCCACGG TGCATGACGT GGATCACCCC 
GGAAGAACCA ACTCCTTCCT GTGTAACGCA GGCAGCGAGC TCGCTGTGCT CTACAACGAC 
ACTGCGGTCT TGGAGAGTCA CCACACAGCC CTGGCCTTCC AGCTCACCGT CAAGGACACC 
AAGTGCAACA TTTTCAAGAA TATCGACAGG AACCACTATC GAACGCTGCG CCAGGCTATT 
ATTGACATGG TTTTGGCCAC AGAGATGACA AAACACTTTG AACATGTGAA CAAGTTTGTG 
AACAGCATCA ACAAGCCTCT AGCAGCCGAG AGTGAGGGCA GCGACTGTGA ATGCAACCCT 
ACTGGGAAGA ACTTTCCCGA AAACCAAATC CTCATCAAGC GTATGATGAT TAAGTGTGCT 
GACGTGGCCA ACCCGTGCCG CCCCTTGGAC CTGTGCATCG AATGGGCTGG AAGGATCTCC 
GAGGAGTACT TTGCACAGAC TGACGAAGAG AAGAGGCAGG GGCTGCCTGT AGTGATGCCA 
GTGTTCGACC GCAATACCTG CAGCATCCCC AAGTCGCAGA TATCCTTCAT CGACTACTTC 
ATAACAGACA TGTTTGATGC TTGGGATGCC TTTGCACACC TGCCAGCCCT GATGCAACAC 
CTGGCCGACA ACTATAAACA CTGGAAGACA CTGGATGACC TCAAGTGCAA AAGTCTGAGA 
CTTCCATCTA ACAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008758892.1
CDS	296..2953
Translation	MGCAPSIHVSQSGVIYCRDSDESNSPRQTSSVSQGPTAPLHGLFVQTDAADAMPPSRAAGPPGAVRVRRSRAELGSGSSTGSSGPAATTCRGRRRHCCSSAEAETQTSYTSVKQVSSAEVRIGPMRLTQDPIQVLLIFAKEDSQSDGFWWACDRAGYRCNIARTPESALECFLDKHHEIIVIDHRQTRNFDAEAVCRSIRATNPSEHTVILAVVSQASDDHEEASVLPLLHAGFNRRFMENSSIIACYNELIQIEHGEVRSQFKLRACNSVFTALDHCHEAIEITSDDHVIQYVNPAFERMMGYHKGELLGKELADLPKSDKNRADLLDTINTCIKKGKEWQGVYYARRKSGDSIQQHVKITPVIGQGGKIRHFVSLKKLCCTTDSSKQIHRIHRDSGDNSQTEPHSFRHKSRRKESIDVKSISSRGSDAPSLQNRRYPSMARIHSMTIEAPITKVINIINAAQENSPVTVAEALDRVLEILRTTELYSPQLGTKDEDPHTSDLVGGLMTDGLRRLSGNEYVFTKNVHHSHSHLSMPITINDVPPSIAQLLDNEESWDFNIFELEAITHKRPLVYLGLKVFSRFGVCEFLNCTETTLRAWLQVIEANYHSSNAYHNSTHAADVLHATAFFLGKERVKGSLDQLDEVAALIAATVHDVDHPGRTNSFLCNAGSELAVLYNDTAVLESHHTALAFQLTVKDTKCNIFKNIDRNHYRTLRQAIIDMVLATEMTKHFEHVNKFVNSINKPLAAESEGSDCECNPTGKNFPENQILIKRMMIKCADVANPCRPLDLCIEWAGRISEEYFAQTDEEKRQGLPVVMPVFDRNTCSIPKSQISFIDYFITDMFDAWDAFAHLPALMQHLADNYKHWKTLDDLKCKSLRLPSNS

Target ORF information:

RefSeq Version	XM_008760670.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus phosphodiesterase 8B (Pde8b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008760670.2

ORF Insert Sequence:

ATGGGCTGCG CCCCCAGCAT CCATGTCTCG CAGAGCGGTG TGATCTACTG CCGGGACTCG 
GATGAGTCCA ACTCGCCCCG CCAGACCAGC AGCGTGTCGC AGGGCCCCAC GGCGCCCCTG 
CACGGCCTCT TCGTGCAGAC CGACGCCGCC GACGCCATGC CCCCGAGTCG CGCGGCGGGA 
CCCCCGGGCG CTGTCCGAGT CCGCAGGTCC CGGGCTGAGC TGGGCAGCGG CAGCAGCACC 
GGGTCCTCGG GGCCCGCGGC GACCACCTGC CGGGGCCGGA GGCGCCACTG CTGCAGCAGC 
GCCGAGGCCG AGACCCAGAC TAGCTACACC AGCGTCAAGC AGGTGTCTTC TGCCGAGGTG 
CGCATTGGGC CCATGAGACT GACACAGGAC CCTATCCAGG TCCTGCTGAT CTTTGCAAAA 
GAAGATAGCC AGAGCGATGG CTTCTGGTGG GCTTGTGACA GAGCTGGCTA CAGATGCAAC 
ATTGCTCGAA CTCCAGAGTC GGCCCTCGAA TGCTTTCTGG ATAAGCATCA TGAGATTATA 
GTGATCGATC ACAGGCAGAC CCGTAACTTT GATGCAGAAG CCGTGTGCAG GTCAATCCGG 
GCCACGAACC CCTCTGAGCA CACAGTGATC CTGGCTGTGG TTTCACAAGC ATCTGATGAC 
CATGAAGAGG CCTCGGTCCT TCCTCTTCTC CATGCAGGCT TTAACCGGAG ATTTATGGAG 
AACAGCAGCA TAATTGCTTG CTATAATGAA CTGATTCAAA TAGAACACGG GGAAGTTCGC 
TCGCAGTTCA AATTACGGGC CTGTAACTCA GTGTTTACAG CGTTAGACCA CTGTCACGAA 
GCCATAGAAA TAACAAGTGA TGACCACGTG ATTCAGTATG TCAACCCTGC CTTTGAGAGG 
ATGATGGGCT ACCACAAAGG GGAGCTCCTG GGGAAGGAAC TCGCTGACCT ACCTAAGAGT 
GACAAGAACC GGGCAGACCT CCTGGACACC ATCAACACGT GCATCAAGAA GGGAAAGGAG 
TGGCAGGGGG TTTACTATGC CAGACGGAAA TCCGGGGACA GCATCCAACA GCACGTGAAG 
ATCACCCCAG TGATTGGCCA AGGAGGGAAA ATTAGGCACT TTGTCTCCCT CAAGAAGCTG 
TGCTGTACCA CTGACAGTAG CAAGCAGATC CACAGGATTC ATCGCGATTC CGGAGACAAC 
TCTCAGACAG AGCCTCATTC GTTCAGACAC AAGAGCAGGA GAAAAGAGTC CATTGATGTG 
AAATCAATAT CTTCTCGAGG CAGTGATGCG CCAAGCCTAC AGAATCGCCG CTACCCCTCC 
ATGGCAAGGA TCCACTCCAT GACCATTGAG GCCCCCATCA CAAAGGTTAT AAATATAATC 
AACGCCGCTC AAGAAAACAG CCCAGTTACA GTGGCAGAAG CCTTGGACAG AGTTCTAGAA 
ATTTTACGAA CCACAGAGCT GTACTCCCCG CAGCTGGGAA CCAAGGACGA AGATCCTCAC 
ACCAGTGACC TTGTCGGAGG CCTGATGACC GATGGCTTGA GGAGACTGTC AGGGAATGAG 
TACGTGTTTA CAAAGAATGT GCACCACAGT CACAGTCACC TCTCGATGCC CATCACCATC 
AACGATGTCC CCCCAAGTAT TGCCCAGCTC CTTGATAATG AGGAAAGTTG GGACTTCAAC 
ATCTTTGAAT TGGAAGCTAT TACACATAAA AGGCCACTTG TGTACCTGGG CTTAAAGGTC 
TTCTCTCGGT TTGGAGTATG TGAATTTTTA AACTGTACCG AAACCACTCT TCGGGCCTGG 
CTGCAAGTGA TTGAAGCAAA CTACCACTCC TCCAATGCCT ACCACAACTC CACCCATGCC 
GCCGACGTAC TGCACGCCAC GGCTTTCTTC CTTGGAAAGG AAAGAGTGAA GGGAAGCCTG 
GACCAGCTGG ATGAGGTGGC GGCTCTGATT GCAGCCACGG TGCATGACGT GGATCACCCC 
GGAAGAACCA ACTCCTTCCT GTGTAACGCA GGCAGCGAGC TCGCTGTGCT CTACAACGAC 
ACTGCGGTCT TGGAGAGTCA CCACACAGCC CTGGCCTTCC AGCTCACCGT CAAGGACACC 
AAGTGCAACA TTTTCAAGAA TATCGACAGG AACCACTATC GAACGCTGCG CCAGGCTATT 
ATTGACATGG TTTTGGCCAC AGAGATGACA AAACACTTTG AACATGTGAA CAAGTTTGTG 
AACAGCATCA ACAAGCCTCT AGCAGCCGAG AGTGAGGGCA GCGACTGTGA ATGCAACCCT 
ACTGGGAAGA ACTTTCCCGA AAACCAAATC CTCATCAAGC GTATGATGAT TAAGTGTGCT 
GACGTGGCCA ACCCGTGCCG CCCCTTGGAC CTGTGCATCG AATGGGCTGG AAGGATCTCC 
GAGGAGTACT TTGCACAGAC TGACGAAGAG AAGAGGCAGG GGCTGCCTGT AGTGATGCCA 
GTGTTCGACC GCAATACCTG CAGCATCCCC AAGTCGCAGA TATCCTTCAT CGACTACTTC 
ATAACAGACA TGTTTGATGC TTGGGATGCC TTTGCACACC TGCCAGCCCT GATGCAACAC 
CTGGCCGACA ACTATAAACA CTGGAAGACA CTGGATGACC TCAAGTGCAA AAGTCTGAGA 
CTTCCATCTA ACAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pde8b ( XM_017590816.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017446305.1 Rattus norvegicus phosphodiesterase 8B (Pde8b), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa59710
Clone ID Related Accession (Same CDS sequence)	XM_017590816.1
Accession Version	XM_017590816.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2655bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	high affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8B isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005101.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCTGCG CCCCCAGCAT CCATGTCTCG CAGAGCGGTG TGATCTACTG CCGGGACTCG 
GATGAGTCCA ACTCGCCCCG CCAGACCAGC AGCGTGTCGC AGGGCCCCAC GGCGCCCCTG 
CACGGCCTCT TCGTGCAGAC CGACGCCGCC GACGCCATGC CCCCGAGTCG CGCGGCGGGA 
CCCCCGGGCG CTGTCCGAGT CCGCAGGTCC CGGGCTGAGC TGGGCAGCGG CAGCAGCACC 
GGGTCCTCGG GGCCCGCGGC GACCACCTGC CGGGGCCGGA GGCGCCACTG CTGCAGCAGC 
GCCGAGGCCG AGACCCAGAC TAGCTACACC AGCGTCAAGG TGTCTTCTGC CGAGGTGCGC 
ATTGGGCCCA TGAGACTGAC ACAGGACCCT ATCCAGGTCC TGCTGATCTT TGCAAAAGAA 
GATAGCCAGA GCGATGGCTT CTGGTGGGCT TGTGACAGAG CTGGCTACAG ATGCAACATT 
GCTCGAACTC CAGAGTCGGC CCTCGAATGC TTTCTGGATA AGCATCATGA GATTATAGTG 
ATCGATCACA GGCAGACCCG TAACTTTGAT GCAGAAGCCG TGTGCAGGTC AATCCGGGCC 
ACGAACCCCT CTGAGCACAC AGTGATCCTG GCTGTGGTTT CACAAGCATC TGATGACCAT 
GAAGAGGCCT CGGTCCTTCC TCTTCTCCAT GCAGGCTTTA ACCGGAGATT TATGGAGAAC 
AGCAGCATAA TTGCTTGCTA TAATGAACTG ATTCAAATAG AACACGGGGA AGTTCGCTCG 
CAGTTCAAAT TACGGGCCTG TAACTCAGTG TTTACAGCGT TAGACCACTG TCACGAAGCC 
ATAGAAATAA CAAGTGATGA CCACGTGATT CAGTATGTCA ACCCTGCCTT TGAGAGGATG 
ATGGGCTACC ACAAAGGGGA GCTCCTGGGG AAGGAACTCG CTGACCTACC TAAGAGTGAC 
AAGAACCGGG CAGACCTCCT GGACACCATC AACACGTGCA TCAAGAAGGG AAAGGAGTGG 
CAGGGGGTTT ACTATGCCAG ACGGAAATCC GGGGACAGCA TCCAACAGCA CGTGAAGATC 
ACCCCAGTGA TTGGCCAAGG AGGGAAAATT AGGCACTTTG TCTCCCTCAA GAAGCTGTGC 
TGTACCACTG ACAGTAGCAA GCAGATCCAC AGGATTCATC GCGATTCCGG AGACAACTCT 
CAGACAGAGC CTCATTCGTT CAGACACAAG AGCAGGAGAA AAGAGTCCAT TGATGTGAAA 
TCAATATCTT CTCGAGGCAG TGATGCGCCA AGCCTACAGA ATCGCCGCTA CCCCTCCATG 
GCAAGGATCC ACTCCATGAC CATTGAGGCC CCCATCACAA AGGTTATAAA TATAATCAAC 
GCCGCTCAAG AAAACAGCCC AGTTACAGTG GCAGAAGCCT TGGACAGAGT TCTAGAAATT 
TTACGAACCA CAGAGCTGTA CTCCCCGCAG CTGGGAACCA AGGACGAAGA TCCTCACACC 
AGTGACCTTG TCGGAGGCCT GATGACCGAT GGCTTGAGGA GACTGTCAGG GAATGAGTAC 
GTGTTTACAA AGAATGTGCA CCACAGTCAC AGTCACCTCT CGATGCCCAT CACCATCAAC 
GATGTCCCCC CAAGTATTGC CCAGCTCCTT GATAATGAGG AAAGTTGGGA CTTCAACATC 
TTTGAATTGG AAGCTATTAC ACATAAAAGG CCACTTGTGT ACCTGGGCTT AAAGGTCTTC 
TCTCGGTTTG GAGTATGTGA ATTTTTAAAC TGTACCGAAA CCACTCTTCG GGCCTGGCTG 
CAAGTGATTG AAGCAAACTA CCACTCCTCC AATGCCTACC ACAACTCCAC CCATGCCGCC 
GACGTACTGC ACGCCACGGC TTTCTTCCTT GGAAAGGAAA GAGTGAAGGG AAGCCTGGAC 
CAGCTGGATG AGGTGGCGGC TCTGATTGCA GCCACGGTGC ATGACGTGGA TCACCCCGGA 
AGAACCAACT CCTTCCTGTG TAACGCAGGC AGCGAGCTCG CTGTGCTCTA CAACGACACT 
GCGGTCTTGG AGAGTCACCA CACAGCCCTG GCCTTCCAGC TCACCGTCAA GGACACCAAG 
TGCAACATTT TCAAGAATAT CGACAGGAAC CACTATCGAA CGCTGCGCCA GGCTATTATT 
GACATGGTTT TGGCCACAGA GATGACAAAA CACTTTGAAC ATGTGAACAA GTTTGTGAAC 
AGCATCAACA AGCCTCTAGC AGCCGAGAGT GAGGGCAGCG ACTGTGAATG CAACCCTACT 
GGGAAGAACT TTCCCGAAAA CCAAATCCTC ATCAAGCGTA TGATGATTAA GTGTGCTGAC 
GTGGCCAACC CGTGCCGCCC CTTGGACCTG TGCATCGAAT GGGCTGGAAG GATCTCCGAG 
GAGTACTTTG CACAGACTGA CGAAGAGAAG AGGCAGGGGC TGCCTGTAGT GATGCCAGTG 
TTCGACCGCA ATACCTGCAG CATCCCCAAG TCGCAGATAT CCTTCATCGA CTACTTCATA 
ACAGACATGT TTGATGCTTG GGATGCCTTT GCACACCTGC CAGCCCTGAT GCAACACCTG 
GCCGACAACT ATAAACACTG GAAGACACTG GATGACCTCA AGTGCAAAAG TCTGAGACTT 
CCATCTAACA GCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017446305.1
CDS	296..2950
Translation	MGCAPSIHVSQSGVIYCRDSDESNSPRQTSSVSQGPTAPLHGLFVQTDAADAMPPSRAAGPPGAVRVRRSRAELGSGSSTGSSGPAATTCRGRRRHCCSSAEAETQTSYTSVKVSSAEVRIGPMRLTQDPIQVLLIFAKEDSQSDGFWWACDRAGYRCNIARTPESALECFLDKHHEIIVIDHRQTRNFDAEAVCRSIRATNPSEHTVILAVVSQASDDHEEASVLPLLHAGFNRRFMENSSIIACYNELIQIEHGEVRSQFKLRACNSVFTALDHCHEAIEITSDDHVIQYVNPAFERMMGYHKGELLGKELADLPKSDKNRADLLDTINTCIKKGKEWQGVYYARRKSGDSIQQHVKITPVIGQGGKIRHFVSLKKLCCTTDSSKQIHRIHRDSGDNSQTEPHSFRHKSRRKESIDVKSISSRGSDAPSLQNRRYPSMARIHSMTIEAPITKVINIINAAQENSPVTVAEALDRVLEILRTTELYSPQLGTKDEDPHTSDLVGGLMTDGLRRLSGNEYVFTKNVHHSHSHLSMPITINDVPPSIAQLLDNEESWDFNIFELEAITHKRPLVYLGLKVFSRFGVCEFLNCTETTLRAWLQVIEANYHSSNAYHNSTHAADVLHATAFFLGKERVKGSLDQLDEVAALIAATVHDVDHPGRTNSFLCNAGSELAVLYNDTAVLESHHTALAFQLTVKDTKCNIFKNIDRNHYRTLRQAIIDMVLATEMTKHFEHVNKFVNSINKPLAAESEGSDCECNPTGKNFPENQILIKRMMIKCADVANPCRPLDLCIEWAGRISEEYFAQTDEEKRQGLPVVMPVFDRNTCSIPKSQISFIDYFITDMFDAWDAFAHLPALMQHLADNYKHWKTLDDLKCKSLRLPSNS

Target ORF information:

RefSeq Version	XM_017590816.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus phosphodiesterase 8B (Pde8b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017590816.1

ORF Insert Sequence:

ATGGGCTGCG CCCCCAGCAT CCATGTCTCG CAGAGCGGTG TGATCTACTG CCGGGACTCG 
GATGAGTCCA ACTCGCCCCG CCAGACCAGC AGCGTGTCGC AGGGCCCCAC GGCGCCCCTG 
CACGGCCTCT TCGTGCAGAC CGACGCCGCC GACGCCATGC CCCCGAGTCG CGCGGCGGGA 
CCCCCGGGCG CTGTCCGAGT CCGCAGGTCC CGGGCTGAGC TGGGCAGCGG CAGCAGCACC 
GGGTCCTCGG GGCCCGCGGC GACCACCTGC CGGGGCCGGA GGCGCCACTG CTGCAGCAGC 
GCCGAGGCCG AGACCCAGAC TAGCTACACC AGCGTCAAGG TGTCTTCTGC CGAGGTGCGC 
ATTGGGCCCA TGAGACTGAC ACAGGACCCT ATCCAGGTCC TGCTGATCTT TGCAAAAGAA 
GATAGCCAGA GCGATGGCTT CTGGTGGGCT TGTGACAGAG CTGGCTACAG ATGCAACATT 
GCTCGAACTC CAGAGTCGGC CCTCGAATGC TTTCTGGATA AGCATCATGA GATTATAGTG 
ATCGATCACA GGCAGACCCG TAACTTTGAT GCAGAAGCCG TGTGCAGGTC AATCCGGGCC 
ACGAACCCCT CTGAGCACAC AGTGATCCTG GCTGTGGTTT CACAAGCATC TGATGACCAT 
GAAGAGGCCT CGGTCCTTCC TCTTCTCCAT GCAGGCTTTA ACCGGAGATT TATGGAGAAC 
AGCAGCATAA TTGCTTGCTA TAATGAACTG ATTCAAATAG AACACGGGGA AGTTCGCTCG 
CAGTTCAAAT TACGGGCCTG TAACTCAGTG TTTACAGCGT TAGACCACTG TCACGAAGCC 
ATAGAAATAA CAAGTGATGA CCACGTGATT CAGTATGTCA ACCCTGCCTT TGAGAGGATG 
ATGGGCTACC ACAAAGGGGA GCTCCTGGGG AAGGAACTCG CTGACCTACC TAAGAGTGAC 
AAGAACCGGG CAGACCTCCT GGACACCATC AACACGTGCA TCAAGAAGGG AAAGGAGTGG 
CAGGGGGTTT ACTATGCCAG ACGGAAATCC GGGGACAGCA TCCAACAGCA CGTGAAGATC 
ACCCCAGTGA TTGGCCAAGG AGGGAAAATT AGGCACTTTG TCTCCCTCAA GAAGCTGTGC 
TGTACCACTG ACAGTAGCAA GCAGATCCAC AGGATTCATC GCGATTCCGG AGACAACTCT 
CAGACAGAGC CTCATTCGTT CAGACACAAG AGCAGGAGAA AAGAGTCCAT TGATGTGAAA 
TCAATATCTT CTCGAGGCAG TGATGCGCCA AGCCTACAGA ATCGCCGCTA CCCCTCCATG 
GCAAGGATCC ACTCCATGAC CATTGAGGCC CCCATCACAA AGGTTATAAA TATAATCAAC 
GCCGCTCAAG AAAACAGCCC AGTTACAGTG GCAGAAGCCT TGGACAGAGT TCTAGAAATT 
TTACGAACCA CAGAGCTGTA CTCCCCGCAG CTGGGAACCA AGGACGAAGA TCCTCACACC 
AGTGACCTTG TCGGAGGCCT GATGACCGAT GGCTTGAGGA GACTGTCAGG GAATGAGTAC 
GTGTTTACAA AGAATGTGCA CCACAGTCAC AGTCACCTCT CGATGCCCAT CACCATCAAC 
GATGTCCCCC CAAGTATTGC CCAGCTCCTT GATAATGAGG AAAGTTGGGA CTTCAACATC 
TTTGAATTGG AAGCTATTAC ACATAAAAGG CCACTTGTGT ACCTGGGCTT AAAGGTCTTC 
TCTCGGTTTG GAGTATGTGA ATTTTTAAAC TGTACCGAAA CCACTCTTCG GGCCTGGCTG 
CAAGTGATTG AAGCAAACTA CCACTCCTCC AATGCCTACC ACAACTCCAC CCATGCCGCC 
GACGTACTGC ACGCCACGGC TTTCTTCCTT GGAAAGGAAA GAGTGAAGGG AAGCCTGGAC 
CAGCTGGATG AGGTGGCGGC TCTGATTGCA GCCACGGTGC ATGACGTGGA TCACCCCGGA 
AGAACCAACT CCTTCCTGTG TAACGCAGGC AGCGAGCTCG CTGTGCTCTA CAACGACACT 
GCGGTCTTGG AGAGTCACCA CACAGCCCTG GCCTTCCAGC TCACCGTCAA GGACACCAAG 
TGCAACATTT TCAAGAATAT CGACAGGAAC CACTATCGAA CGCTGCGCCA GGCTATTATT 
GACATGGTTT TGGCCACAGA GATGACAAAA CACTTTGAAC ATGTGAACAA GTTTGTGAAC 
AGCATCAACA AGCCTCTAGC AGCCGAGAGT GAGGGCAGCG ACTGTGAATG CAACCCTACT 
GGGAAGAACT TTCCCGAAAA CCAAATCCTC ATCAAGCGTA TGATGATTAA GTGTGCTGAC 
GTGGCCAACC CGTGCCGCCC CTTGGACCTG TGCATCGAAT GGGCTGGAAG GATCTCCGAG 
GAGTACTTTG CACAGACTGA CGAAGAGAAG AGGCAGGGGC TGCCTGTAGT GATGCCAGTG 
TTCGACCGCA ATACCTGCAG CATCCCCAAG TCGCAGATAT CCTTCATCGA CTACTTCATA 
ACAGACATGT TTGATGCTTG GGATGCCTTT GCACACCTGC CAGCCCTGAT GCAACACCTG 
GCCGACAACT ATAAACACTG GAAGACACTG GATGACCTCA AGTGCAAAAG TCTGAGACTT 
CCATCTAACA GCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Pde8b ( XM_017590817.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017446306.1 Rattus norvegicus phosphodiesterase 8B (Pde8b), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ORa59711
Clone ID Related Accession (Same CDS sequence)	XM_017590817.1
Accession Version	XM_017590817.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2508bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	high affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8B isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005101.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCTGCG CCCCCAGCAT CCATGTCTCG CAGAGCGGTG TGATCTACTG CCGGGACTCG 
GATGAGTCCA ACTCGCCCCG CCAGACCAGC AGCGTGTCGC AGGGCCCCAC GGCGCCCCTG 
CACGGCCTCT TCGTGCAGAC CGACGCCGCC GACGCCATGC CCCCGAGTCG CGCGGCGGGA 
CCCCCGGGCG CTGTCCGAGT CCGCAGGTCC CGGGCTGAGC TGGGCAGCGG CAGCAGCACC 
GGGTCCTCGG GGCCCGCGGC GACCACCTGC CGGGGCCGGA GGCGCCACTG CTGCAGCAGC 
GCCGAGGCCG AGACCCAGAC TAGCTACACC AGCGTCAAGC AGGTGTCTTC TGCCGAGGTG 
CGCATTGGGC CCATGAGACT GACACAGGAC CCTATCCAGG TCCTGCTGAT CTTTGCAAAA 
GAAGATAGCC AGAGCGATGG CTTCTGGTGG GCTTGTGACA GAGCTGGCTA CAGATGCAAC 
ATTGCTCGAA CTCCAGAGTC GGCCCTCGAA TGCTTTCTGG ATAAGCATCA TGAGATTATA 
GTGATCGATC ACAGGCAGAC CCGTAACTTT GATGCAGAAG CCGTGTGCAG GTCAATCCGG 
GCCACGAACC CCTCTGAGCA CACAGTGATC CTGGCTGTGG TTTCACAAGC ATCTGATGAC 
CATGAAGAGG CCTCGGTCCT TCCTCTTCTC CATGCAGGCT TTAACCGGAG ATTTATGGAG 
AACAGCAGCA TAATTGCTTG CTATAATGAA CTGATTCAAA TAGAACACGG GGAAGTTCGC 
TCGCAGTTCA AATTACGGGC CTGTAACTCA GTGTTTACAG CGTTAGACCA CTGTCACGAA 
GCCATAGAAA TAACAAGTGA TGACCACGTG ATTCAGTATG TCAACCCTGC CTTTGAGAGG 
ATGATGGGCT ACCACAAAGG GGAGCTCCTG GGGAAGGAAC TCGCTGACCT ACCTAAGAGT 
GACAAGAACC GGGCAGACCT CCTGGACACC ATCAACACGT GCATCAAGAA GGGAAAGATC 
CACAGGATTC ATCGCGATTC CGGAGACAAC TCTCAGACAG AGCCTCATTC GTTCAGACAC 
AAGAGCAGGA GAAAAGAGTC CATTGATGTG AAATCAATAT CTTCTCGAGG CAGTGATGCG 
CCAAGCCTAC AGAATCGCCG CTACCCCTCC ATGGCAAGGA TCCACTCCAT GACCATTGAG 
GCCCCCATCA CAAAGGTTAT AAATATAATC AACGCCGCTC AAGAAAACAG CCCAGTTACA 
GTGGCAGAAG CCTTGGACAG AGTTCTAGAA ATTTTACGAA CCACAGAGCT GTACTCCCCG 
CAGCTGGGAA CCAAGGACGA AGATCCTCAC ACCAGTGACC TTGTCGGAGG CCTGATGACC 
GATGGCTTGA GGAGACTGTC AGGGAATGAG TACGTGTTTA CAAAGAATGT GCACCACAGT 
CACAGTCACC TCTCGATGCC CATCACCATC AACGATGTCC CCCCAAGTAT TGCCCAGCTC 
CTTGATAATG AGGAAAGTTG GGACTTCAAC ATCTTTGAAT TGGAAGCTAT TACACATAAA 
AGGCCACTTG TGTACCTGGG CTTAAAGGTC TTCTCTCGGT TTGGAGTATG TGAATTTTTA 
AACTGTACCG AAACCACTCT TCGGGCCTGG CTGCAAGTGA TTGAAGCAAA CTACCACTCC 
TCCAATGCCT ACCACAACTC CACCCATGCC GCCGACGTAC TGCACGCCAC GGCTTTCTTC 
CTTGGAAAGG AAAGAGTGAA GGGAAGCCTG GACCAGCTGG ATGAGGTGGC GGCTCTGATT 
GCAGCCACGG TGCATGACGT GGATCACCCC GGAAGAACCA ACTCCTTCCT GTGTAACGCA 
GGCAGCGAGC TCGCTGTGCT CTACAACGAC ACTGCGGTCT TGGAGAGTCA CCACACAGCC 
CTGGCCTTCC AGCTCACCGT CAAGGACACC AAGTGCAACA TTTTCAAGAA TATCGACAGG 
AACCACTATC GAACGCTGCG CCAGGCTATT ATTGACATGG TTTTGGCCAC AGAGATGACA 
AAACACTTTG AACATGTGAA CAAGTTTGTG AACAGCATCA ACAAGCCTCT AGCAGCCGAG 
AGTGAGGGCA GCGACTGTGA ATGCAACCCT ACTGGGAAGA ACTTTCCCGA AAACCAAATC 
CTCATCAAGC GTATGATGAT TAAGTGTGCT GACGTGGCCA ACCCGTGCCG CCCCTTGGAC 
CTGTGCATCG AATGGGCTGG AAGGATCTCC GAGGAGTACT TTGCACAGAC TGACGAAGAG 
AAGAGGCAGG GGCTGCCTGT AGTGATGCCA GTGTTCGACC GCAATACCTG CAGCATCCCC 
AAGTCGCAGA TATCCTTCAT CGACTACTTC ATAACAGACA TGTTTGATGC TTGGGATGCC 
TTTGCACACC TGCCAGCCCT GATGCAACAC CTGGCCGACA ACTATAAACA CTGGAAGACA 
CTGGATGACC TCAAGTGCAA AAGTCTGAGA CTTCCATCTA ACAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017446306.1
CDS	296..2803
Translation	MGCAPSIHVSQSGVIYCRDSDESNSPRQTSSVSQGPTAPLHGLFVQTDAADAMPPSRAAGPPGAVRVRRSRAELGSGSSTGSSGPAATTCRGRRRHCCSSAEAETQTSYTSVKQVSSAEVRIGPMRLTQDPIQVLLIFAKEDSQSDGFWWACDRAGYRCNIARTPESALECFLDKHHEIIVIDHRQTRNFDAEAVCRSIRATNPSEHTVILAVVSQASDDHEEASVLPLLHAGFNRRFMENSSIIACYNELIQIEHGEVRSQFKLRACNSVFTALDHCHEAIEITSDDHVIQYVNPAFERMMGYHKGELLGKELADLPKSDKNRADLLDTINTCIKKGKIHRIHRDSGDNSQTEPHSFRHKSRRKESIDVKSISSRGSDAPSLQNRRYPSMARIHSMTIEAPITKVINIINAAQENSPVTVAEALDRVLEILRTTELYSPQLGTKDEDPHTSDLVGGLMTDGLRRLSGNEYVFTKNVHHSHSHLSMPITINDVPPSIAQLLDNEESWDFNIFELEAITHKRPLVYLGLKVFSRFGVCEFLNCTETTLRAWLQVIEANYHSSNAYHNSTHAADVLHATAFFLGKERVKGSLDQLDEVAALIAATVHDVDHPGRTNSFLCNAGSELAVLYNDTAVLESHHTALAFQLTVKDTKCNIFKNIDRNHYRTLRQAIIDMVLATEMTKHFEHVNKFVNSINKPLAAESEGSDCECNPTGKNFPENQILIKRMMIKCADVANPCRPLDLCIEWAGRISEEYFAQTDEEKRQGLPVVMPVFDRNTCSIPKSQISFIDYFITDMFDAWDAFAHLPALMQHLADNYKHWKTLDDLKCKSLRLPSNS

Target ORF information:

RefSeq Version	XM_017590817.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus phosphodiesterase 8B (Pde8b), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017590817.1

ORF Insert Sequence:

ATGGGCTGCG CCCCCAGCAT CCATGTCTCG CAGAGCGGTG TGATCTACTG CCGGGACTCG 
GATGAGTCCA ACTCGCCCCG CCAGACCAGC AGCGTGTCGC AGGGCCCCAC GGCGCCCCTG 
CACGGCCTCT TCGTGCAGAC CGACGCCGCC GACGCCATGC CCCCGAGTCG CGCGGCGGGA 
CCCCCGGGCG CTGTCCGAGT CCGCAGGTCC CGGGCTGAGC TGGGCAGCGG CAGCAGCACC 
GGGTCCTCGG GGCCCGCGGC GACCACCTGC CGGGGCCGGA GGCGCCACTG CTGCAGCAGC 
GCCGAGGCCG AGACCCAGAC TAGCTACACC AGCGTCAAGC AGGTGTCTTC TGCCGAGGTG 
CGCATTGGGC CCATGAGACT GACACAGGAC CCTATCCAGG TCCTGCTGAT CTTTGCAAAA 
GAAGATAGCC AGAGCGATGG CTTCTGGTGG GCTTGTGACA GAGCTGGCTA CAGATGCAAC 
ATTGCTCGAA CTCCAGAGTC GGCCCTCGAA TGCTTTCTGG ATAAGCATCA TGAGATTATA 
GTGATCGATC ACAGGCAGAC CCGTAACTTT GATGCAGAAG CCGTGTGCAG GTCAATCCGG 
GCCACGAACC CCTCTGAGCA CACAGTGATC CTGGCTGTGG TTTCACAAGC ATCTGATGAC 
CATGAAGAGG CCTCGGTCCT TCCTCTTCTC CATGCAGGCT TTAACCGGAG ATTTATGGAG 
AACAGCAGCA TAATTGCTTG CTATAATGAA CTGATTCAAA TAGAACACGG GGAAGTTCGC 
TCGCAGTTCA AATTACGGGC CTGTAACTCA GTGTTTACAG CGTTAGACCA CTGTCACGAA 
GCCATAGAAA TAACAAGTGA TGACCACGTG ATTCAGTATG TCAACCCTGC CTTTGAGAGG 
ATGATGGGCT ACCACAAAGG GGAGCTCCTG GGGAAGGAAC TCGCTGACCT ACCTAAGAGT 
GACAAGAACC GGGCAGACCT CCTGGACACC ATCAACACGT GCATCAAGAA GGGAAAGATC 
CACAGGATTC ATCGCGATTC CGGAGACAAC TCTCAGACAG AGCCTCATTC GTTCAGACAC 
AAGAGCAGGA GAAAAGAGTC CATTGATGTG AAATCAATAT CTTCTCGAGG CAGTGATGCG 
CCAAGCCTAC AGAATCGCCG CTACCCCTCC ATGGCAAGGA TCCACTCCAT GACCATTGAG 
GCCCCCATCA CAAAGGTTAT AAATATAATC AACGCCGCTC AAGAAAACAG CCCAGTTACA 
GTGGCAGAAG CCTTGGACAG AGTTCTAGAA ATTTTACGAA CCACAGAGCT GTACTCCCCG 
CAGCTGGGAA CCAAGGACGA AGATCCTCAC ACCAGTGACC TTGTCGGAGG CCTGATGACC 
GATGGCTTGA GGAGACTGTC AGGGAATGAG TACGTGTTTA CAAAGAATGT GCACCACAGT 
CACAGTCACC TCTCGATGCC CATCACCATC AACGATGTCC CCCCAAGTAT TGCCCAGCTC 
CTTGATAATG AGGAAAGTTG GGACTTCAAC ATCTTTGAAT TGGAAGCTAT TACACATAAA 
AGGCCACTTG TGTACCTGGG CTTAAAGGTC TTCTCTCGGT TTGGAGTATG TGAATTTTTA 
AACTGTACCG AAACCACTCT TCGGGCCTGG CTGCAAGTGA TTGAAGCAAA CTACCACTCC 
TCCAATGCCT ACCACAACTC CACCCATGCC GCCGACGTAC TGCACGCCAC GGCTTTCTTC 
CTTGGAAAGG AAAGAGTGAA GGGAAGCCTG GACCAGCTGG ATGAGGTGGC GGCTCTGATT 
GCAGCCACGG TGCATGACGT GGATCACCCC GGAAGAACCA ACTCCTTCCT GTGTAACGCA 
GGCAGCGAGC TCGCTGTGCT CTACAACGAC ACTGCGGTCT TGGAGAGTCA CCACACAGCC 
CTGGCCTTCC AGCTCACCGT CAAGGACACC AAGTGCAACA TTTTCAAGAA TATCGACAGG 
AACCACTATC GAACGCTGCG CCAGGCTATT ATTGACATGG TTTTGGCCAC AGAGATGACA 
AAACACTTTG AACATGTGAA CAAGTTTGTG AACAGCATCA ACAAGCCTCT AGCAGCCGAG 
AGTGAGGGCA GCGACTGTGA ATGCAACCCT ACTGGGAAGA ACTTTCCCGA AAACCAAATC 
CTCATCAAGC GTATGATGAT TAAGTGTGCT GACGTGGCCA ACCCGTGCCG CCCCTTGGAC 
CTGTGCATCG AATGGGCTGG AAGGATCTCC GAGGAGTACT TTGCACAGAC TGACGAAGAG 
AAGAGGCAGG GGCTGCCTGT AGTGATGCCA GTGTTCGACC GCAATACCTG CAGCATCCCC 
AAGTCGCAGA TATCCTTCAT CGACTACTTC ATAACAGACA TGTTTGATGC TTGGGATGCC 
TTTGCACACC TGCCAGCCCT GATGCAACAC CTGGCCGACA ACTATAAACA CTGGAAGACA 
CTGGATGACC TCAAGTGCAA AAGTCTGAGA CTTCCATCTA ACAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Inactivation of Pde8b enhances memory, motor performance, and protects against age-induced motor coordination decay.
Genes, brain, and behavior11(7)837-47(2012 Oct)
Tsai LC,Chan GC,Nangle SN,Shimizu-Albergine M,Jones GL,Storm DR,Beavo JA,Zweifel LS

The high-affinity cAMP-specific phosphodiesterase 8B controls steroidogenesis in the mouse adrenal gland.
Molecular pharmacology79(4)639-48(2011 Apr)
Tsai LC,Shimizu-Albergine M,Beavo JA

Phosphodiesterase 3 and 4 comprise the major cAMP metabolizing enzymes responsible for insulin secretion in INS-1 (832/13) cells and rat islets.
Biochemical pharmacology76(7)884-93(2008 Oct)
Waddleton D,Wu W,Feng Y,Thompson C,Wu M,Zhou YP,Howard A,Thornberry N,Li J,Mancini JA

Diminished phosphodiesterase-8B potentiates biphasic insulin response to glucose.
Endocrinology149(2)741-8(2008 Feb)
Dov A,Abramovitch E,Warwar N,Nesher R

Biochemistry and physiology of cyclic nucleotide phosphodiesterases: essential components in cyclic nucleotide signaling.
Annual review of biochemistry76481-511(2007)
Conti M,Beavo J

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Pde8b cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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