Rbm20 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Rbm20
Entrez Gene ID	309544
Full Name	RNA binding motif protein 20
General protein information	Preferred Names RNA binding motif protein 20 Names RNA-binding protein 20 probable RNA-binding protein 20
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 1 Map Location: 1q55

mRNA and Protein(s)

mRNA	Protein	Name
XM_017589306.1	XP_017444795.1	RNA-binding protein 20 isoform X1
NM_001107611.2	NP_001101081.2	RNA-binding protein 20

Pathways from BioSystems

Homologies

Homo sapiens (human)	RBM20	NP_001127835.1
Pan troglodytes (chimpanzee)	RBM20	XP_508032.3
Canis lupus familiaris (dog)	RBM20	XP_544017.3
Gallus gallus (chicken)	RBM20	XP_421755.4
Bos taurus (cattle)	RBM20	NP_001179542.1
Mus musculus (house mouse)	Rbm20	NP_001164318.1
Xenopus tropicalis (tropical clawed frog)	rbm20	XP_002942947.2
	RBM20	XP_001087170.2
Rattus norvegicus (Norway rat)	Rbm20	NP_001101081.2
Danio rerio (zebrafish)	LOC555579	XP_683222.5

Gene Ontology
Bibliography

Related articles in PubMed

Insulin regulates titin pre-mRNA splicing through the PI3K-Akt-mTOR kinase axis in a RBM20-dependent manner.
Zhu C, Yin Z, Tan B, Guo W
Biochimica et biophysica acta1863(9)2363-2371(2017 Sep)

RBM20 and RBM24 cooperatively promote the expression of short enh splice variants.
Ito J, Iijima M, Yoshimoto N, Niimi T, Kuroda S, Maturana AD
FEBS letters590(14)2262-74(2016 07)

RNA-binding protein RBM20 represses splicing to orchestrate cardiac pre-mRNA processing.
Maatz H, Jens M, Liss M, Schafer S, Heinig M, Kirchner M, Adami E, Rintisch C, Dauksaite V, Radke MH, Selbach M, Barton PJ, Cook SA, Rajewsky N, Gotthardt M, Landthaler M, Hubner N
The Journal of clinical investigation124(8)3419-30(2014 Aug)

Pathophysiological defects and transcriptional profiling in the RBM20-/- rat model.
Guo W, Pleitner JM, Saupe KW, Greaser ML
PloS one8(12)e84281(2013)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

results suggest that, in healthy conditions, RBM20 and RBM24 cooperate to promote the expression of short ENH isoforms.
Title: RBM20 and RBM24 cooperatively promote the expression of short enh splice variants.

combined transcriptome-wide crosslinking immunoprecipitation, RNA-seq, and quantitative proteomics in cell culture and hearts to examine how RBM20 regulates alternative splicing in the heart
Title: RNA-binding protein RBM20 represses splicing to orchestrate cardiac pre-mRNA processing.

new insights on Rbm20 function and how its malfunction leads to cardiomyopathy
Title: Pathophysiological defects and transcriptional profiling in the RBM20-/- rat model.

Deep sequencing of the human and rat cardiac transcriptome revealed an RBM20-dependent regulation of alternative splicing
Title: RBM20, a gene for hereditary cardiomyopathy, regulates titin splicing.

The following Rbm20 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Rbm20 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa56654	XM_017589306.1 Latest version!	Rattus norvegicus RNA binding motif protein 20 (Rbm20), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
ORa11400	NM_001107611.2 Latest version!	Rattus norvegicus RNA binding motif protein 20 (Rbm20), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Rbm20 ( XM_017589306.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017444795.1 Rattus norvegicus RNA binding motif protein 20 (Rbm20), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa56654
Clone ID Related Accession (Same CDS sequence)	XM_017589306.1
Accession Version	XM_017589306.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3312bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	RNA-binding protein 20 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCACAGA CCGCAGTCAC CAACAACACT GCAGCCGCCA CTGTCCTCAA CCAAGTCCTC 
TCCAAAGTGG CCATGTCCCA GCCTCTCTTC AACCAGCTTC GCCATCCGTC TGTGCTTGGC 
ACCACACATG GCCCTACTGG GGTCTCCCAG CATGCTGCCA CCGTTCCCAG TGCTCACTTT 
CCCTCAACTG CAATCGCCTT TTCGCCCCCA AGCCAGGCAG GAGGCCCGGG GCCTTCTGTG 
AGCCTCCCCA GCCAGCCCCC CAATGCTATG GTAGTGCATA CCTTCAGTGG GGTGGTGCCT 
CAGACCCCTG CCCAGCCAGC AGTCATCCTA AGCATTGGGA AGGCGGGGCC AACACCTGCT 
ACTACAGGGT TCTATGACTA TGGCAAAGCC AACCCTGGCC AGGCCTATGG TTCTGAAACG 
GAGGGCCAGC CGGGCTTCTT GCCAGCCTCG GCGTCAGCCG CAGCATCAGG CGGTGTGACC 
TATGAAGGAC ACTATAGCCA CACAGGGCAG GATGGCCAAG CCACCTTTTC TAAAGACTTC 
TATGGACCCA GTGCCCAAGG GTCACATGCA GCAGGTGGGT TCCCAGCTGA TCAGGCTGGG 
AGCATGAAAG GAGACGTCGG TGGGTTGTTG CAAGGTACCA ACAGCCAGTG GGAGAGGCCC 
TCTGGGTTCT CTGGCCAGAA CAAGGCCGAT ATTACAGCCG GGCCCGGTTT GTGGGCTCCA 
CCTGCCAGTC AGCCTTATGA ACTATATGAT CCTGAGGAGC CTACCTCAGA CAGGGCCCCT 
CCTGCCTTTG GATCTCGACT TAACAACAGC AAGCAGGGAT TCAACTGCTC CTGCCGGCGG 
ACAAAGGAGG GGCAGGCCAT GCTGTCCGTG AGGCCCCTGC AGGGTCATCA ACTGAATGAC 
TTCCGAGGCT TGGCTCCACT CCACCTTCCA CATATCTGCA GCATCTGTGA CAAGAAGGTG 
TTTGACCTGA AGGACTGGGA GCTACATGTG AAGGGGAAGT TGCATGCCCA GAAATGCCTG 
CTCTTCTCAG AAAGTGCTGG CCTCCGGAGT ATATGTGCTA CAGGAGAAGG GACGCTGTCT 
GCCTCTGCAA ACAGCACAGC TGTTTATAAC CCCACTGGAA ATGAGGATTA TACCTCAACT 
CTTGGAACAT CATATGCAGC CATTCCAACA AGGGCCTTTG CTCAATCAAA CCCCATGTTT 
CCTTCGGCTT CCTCTGGGAC AAATTTTGCA CAGAGGAAAG GTGCTGGACG AGTTGTGCAC 
ATCTGCAATC TCCCAGAGGG CAGCTGCACG GAGAATGACG TCATCAACCT GGGGCTGCCC 
TTTGGCAAGG TCACTAATTA CATCCTTATG AAGTCAACTA ATCAGGCTTT CTTGGAGATG 
GCTTACACAG AAGCTGCTCA AGCTATGGTC CAGTACTACC AAGAAAAGCC CGCGCTTATC 
AATGGCGAGA AGTTACTCAT TCGGATGTCC ACGAGATACA AGGAATTGCA GCTGAAGAAA 
CCTGGGAAAA ATGTGGCTGC TATCATCCAG GACATCCACT CCCAAAGGGA GAGGTGCATG 
CTCCGGGAAG CTGACAGATA TGGTCCAGAG CGACCACGTT CTCGAAGTCC AATGAGCCGA 
TCGCTCTCCC CGAGATCCCA CAGTCCTCCT GGCCCCTCTC GGGCTGATTG GGGCAATGGC 
CGTGACTCCT ACGCATGGAG GGACGAGGAT CGGGAGACGG TGCCCAGGAG GGAGAACGGG 
GAAGACAAGA GGGACAGGTT GGATGTTTGG GCACATGACC GGAAACACTA TCCCAGGCAG 
CTGGACAAAG CCGAGTTGGA TGAGCGACTC GAAGGAGGGA GGGGCTACCG GGAAAAGTAC 
TTGAAGTCAG GGTCCCCCGG CCCACTCCAT TCTGCGTCTG GCTACAAAGG CCGGGAAGAT 
GGCTACCATC GAAAAGAGAC TAAAGCTAAG TTGGACAAAC ACCCAAAGCA GCAGCAGCAG 
GATGTGCCAG GAAGATCCAG GAGGAAAGAA GAGGCGCGAC TACGGGAGCC CAGACACCCT 
CACCCAGAGG ACTCTGGCAA GGAAGAGGAT CTGGAGCCCA AGGTCACTCG GGCCCCCGAG 
GGTACCAAGT CCAAGCAAAG TGAGAAAAGT AAAACCAAGA GAGCCGACAG AGACCAAGAA 
GGAGCTGATG ACAAAAAAGA AGGCCGAGGG GCAGAGAATG AGGCTGGGAC TGAGGAACAG 
GAAGGCATGG AAGAGAGCCC CGCATCGGTG GGCACACAGC AGGAAGGGAC GGAGTCCTCC 
GATCCAGAAA ACACAAGGAC AAAGAAGGGA CAAGACTGTG ACAGTGGAAG TGAGCCTGAG 
GGGGACAACT GGTACCCCAC CAACATGGAG GAGCTGGTCA CAGTGGACGA AGTAGGGGAG 
GAAGACTTCA TCATGGAGCC AGACATACCG GAGCTGGAAG AAATTGTACC CATCGACCAG 
AAAGACAAAA TCCTCCCCGA AATATGTCCC TGTGTAACAG CCACCTTAGG TTTGGACTTG 
GCCAAAGACT TCACCAAGCA GGGAGAGACC CTAGGGAACG GAGACGCAGA ACTCAGCCCG 
AAGCTGCCCG GACAAGTGCC GTCTACTTCC ACAAGCTGTC CCAATGACAC GGACATGGAG 
ATGGCTGGCC TAAATCTGGA TGCTGAGCGG AAGCCAGCTG AAAGCGAGAC AGGCCTCTCA 
CCGGAGGTCT CAAATTGCTA CGAGAAGGAG GCGAGAGGAG CGGAGGGCTC AGATGTGCGT 
CTGGCCCCTG CAGCCCAGCG AATGTCCTCC CCTCAGCCAG CAGATGAGAG GGCCCAGCAG 
TCCAGCCCTT TTCTCGATGA CTGCAAGGCC AGGGGGTCCC CAGAAGATGG GCCTCATGAA 
GTCAGCCCCC TGGAAGAAAA AGCCAGCCCC ACTACTGAAA GCGACCTCCA AAGCCAGGCT 
TGCCAAGAAA ACTCCAGGTA CACGGAGACG AGATCTCTGA ACTCGAGATC ACCAGAATTC 
ACCGAAGCGG AGCTGAAAGA GCCCCTTTCT TTGCCTTCCT GGGAACCAGA GGTGTTCAGT 
GAACTTAGCA TTCCTCTAGG AGTGGAGTTC GTGGTTCCCA GGACTGGCTT TTACTGCAAG 
CTGTGTGGCC TGTTCTACAC AAGCGAGGAG GCGGCCAAAG TGAGCCACTG CCGCAGCACT 
GTACACTACA GGAACTTACA GAAGTACCTG TCCCAGCTGG CAGAGGAGGG ACTGAAGGAG 
ACAGAGGGGG TAGACAGCCC AAGCCCCGAG CGCAGCGGGA TTGGTCCACA CTTGGAAAGG 
AAGAAGCTAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017444795.1
CDS	2872..6183
Translation	MAQTAVTNNTAAATVLNQVLSKVAMSQPLFNQLRHPSVLGTTHGPTGVSQHAATVPSAHFPSTAIAFSPPSQAGGPGPSVSLPSQPPNAMVVHTFSGVVPQTPAQPAVILSIGKAGPTPATTGFYDYGKANPGQAYGSETEGQPGFLPASASAAASGGVTYEGHYSHTGQDGQATFSKDFYGPSAQGSHAAGGFPADQAGSMKGDVGGLLQGTNSQWERPSGFSGQNKADITAGPGLWAPPASQPYELYDPEEPTSDRAPPAFGSRLNNSKQGFNCSCRRTKEGQAMLSVRPLQGHQLNDFRGLAPLHLPHICSICDKKVFDLKDWELHVKGKLHAQKCLLFSESAGLRSICATGEGTLSASANSTAVYNPTGNEDYTSTLGTSYAAIPTRAFAQSNPMFPSASSGTNFAQRKGAGRVVHICNLPEGSCTENDVINLGLPFGKVTNYILMKSTNQAFLEMAYTEAAQAMVQYYQEKPALINGEKLLIRMSTRYKELQLKKPGKNVAAIIQDIHSQRERCMLREADRYGPERPRSRSPMSRSLSPRSHSPPGPSRADWGNGRDSYAWRDEDRETVPRRENGEDKRDRLDVWAHDRKHYPRQLDKAELDERLEGGRGYREKYLKSGSPGPLHSASGYKGREDGYHRKETKAKLDKHPKQQQQDVPGRSRRKEEARLREPRHPHPEDSGKEEDLEPKVTRAPEGTKSKQSEKSKTKRADRDQEGADDKKEGRGAENEAGTEEQEGMEESPASVGTQQEGTESSDPENTRTKKGQDCDSGSEPEGDNWYPTNMEELVTVDEVGEEDFIMEPDIPELEEIVPIDQKDKILPEICPCVTATLGLDLAKDFTKQGETLGNGDAELSPKLPGQVPSTSTSCPNDTDMEMAGLNLDAERKPAESETGLSPEVSNCYEKEARGAEGSDVRLAPAAQRMSSPQPADERAQQSSPFLDDCKARGSPEDGPHEVSPLEEKASPTTESDLQSQACQENSRYTETRSLNSRSPEFTEAELKEPLSLPSWEPEVFSELSIPLGVEFVVPRTGFYCKLCGLFYTSEEAAKVSHCRSTVHYRNLQKYLSQLAEEGLKETEGVDSPSPERSGIGPHLERKKL

Target ORF information:

RefSeq Version	XM_017589306.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus RNA binding motif protein 20 (Rbm20), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017589306.1

ORF Insert Sequence:

ATGGCACAGA CCGCAGTCAC CAACAACACT GCAGCCGCCA CTGTCCTCAA CCAAGTCCTC 
TCCAAAGTGG CCATGTCCCA GCCTCTCTTC AACCAGCTTC GCCATCCGTC TGTGCTTGGC 
ACCACACATG GCCCTACTGG GGTCTCCCAG CATGCTGCCA CCGTTCCCAG TGCTCACTTT 
CCCTCAACTG CAATCGCCTT TTCGCCCCCA AGCCAGGCAG GAGGCCCGGG GCCTTCTGTG 
AGCCTCCCCA GCCAGCCCCC CAATGCTATG GTAGTGCATA CCTTCAGTGG GGTGGTGCCT 
CAGACCCCTG CCCAGCCAGC AGTCATCCTA AGCATTGGGA AGGCGGGGCC AACACCTGCT 
ACTACAGGGT TCTATGACTA TGGCAAAGCC AACCCTGGCC AGGCCTATGG TTCTGAAACG 
GAGGGCCAGC CGGGCTTCTT GCCAGCCTCG GCGTCAGCCG CAGCATCAGG CGGTGTGACC 
TATGAAGGAC ACTATAGCCA CACAGGGCAG GATGGCCAAG CCACCTTTTC TAAAGACTTC 
TATGGACCCA GTGCCCAAGG GTCACATGCA GCAGGTGGGT TCCCAGCTGA TCAGGCTGGG 
AGCATGAAAG GAGACGTCGG TGGGTTGTTG CAAGGTACCA ACAGCCAGTG GGAGAGGCCC 
TCTGGGTTCT CTGGCCAGAA CAAGGCCGAT ATTACAGCCG GGCCCGGTTT GTGGGCTCCA 
CCTGCCAGTC AGCCTTATGA ACTATATGAT CCTGAGGAGC CTACCTCAGA CAGGGCCCCT 
CCTGCCTTTG GATCTCGACT TAACAACAGC AAGCAGGGAT TCAACTGCTC CTGCCGGCGG 
ACAAAGGAGG GGCAGGCCAT GCTGTCCGTG AGGCCCCTGC AGGGTCATCA ACTGAATGAC 
TTCCGAGGCT TGGCTCCACT CCACCTTCCA CATATCTGCA GCATCTGTGA CAAGAAGGTG 
TTTGACCTGA AGGACTGGGA GCTACATGTG AAGGGGAAGT TGCATGCCCA GAAATGCCTG 
CTCTTCTCAG AAAGTGCTGG CCTCCGGAGT ATATGTGCTA CAGGAGAAGG GACGCTGTCT 
GCCTCTGCAA ACAGCACAGC TGTTTATAAC CCCACTGGAA ATGAGGATTA TACCTCAACT 
CTTGGAACAT CATATGCAGC CATTCCAACA AGGGCCTTTG CTCAATCAAA CCCCATGTTT 
CCTTCGGCTT CCTCTGGGAC AAATTTTGCA CAGAGGAAAG GTGCTGGACG AGTTGTGCAC 
ATCTGCAATC TCCCAGAGGG CAGCTGCACG GAGAATGACG TCATCAACCT GGGGCTGCCC 
TTTGGCAAGG TCACTAATTA CATCCTTATG AAGTCAACTA ATCAGGCTTT CTTGGAGATG 
GCTTACACAG AAGCTGCTCA AGCTATGGTC CAGTACTACC AAGAAAAGCC CGCGCTTATC 
AATGGCGAGA AGTTACTCAT TCGGATGTCC ACGAGATACA AGGAATTGCA GCTGAAGAAA 
CCTGGGAAAA ATGTGGCTGC TATCATCCAG GACATCCACT CCCAAAGGGA GAGGTGCATG 
CTCCGGGAAG CTGACAGATA TGGTCCAGAG CGACCACGTT CTCGAAGTCC AATGAGCCGA 
TCGCTCTCCC CGAGATCCCA CAGTCCTCCT GGCCCCTCTC GGGCTGATTG GGGCAATGGC 
CGTGACTCCT ACGCATGGAG GGACGAGGAT CGGGAGACGG TGCCCAGGAG GGAGAACGGG 
GAAGACAAGA GGGACAGGTT GGATGTTTGG GCACATGACC GGAAACACTA TCCCAGGCAG 
CTGGACAAAG CCGAGTTGGA TGAGCGACTC GAAGGAGGGA GGGGCTACCG GGAAAAGTAC 
TTGAAGTCAG GGTCCCCCGG CCCACTCCAT TCTGCGTCTG GCTACAAAGG CCGGGAAGAT 
GGCTACCATC GAAAAGAGAC TAAAGCTAAG TTGGACAAAC ACCCAAAGCA GCAGCAGCAG 
GATGTGCCAG GAAGATCCAG GAGGAAAGAA GAGGCGCGAC TACGGGAGCC CAGACACCCT 
CACCCAGAGG ACTCTGGCAA GGAAGAGGAT CTGGAGCCCA AGGTCACTCG GGCCCCCGAG 
GGTACCAAGT CCAAGCAAAG TGAGAAAAGT AAAACCAAGA GAGCCGACAG AGACCAAGAA 
GGAGCTGATG ACAAAAAAGA AGGCCGAGGG GCAGAGAATG AGGCTGGGAC TGAGGAACAG 
GAAGGCATGG AAGAGAGCCC CGCATCGGTG GGCACACAGC AGGAAGGGAC GGAGTCCTCC 
GATCCAGAAA ACACAAGGAC AAAGAAGGGA CAAGACTGTG ACAGTGGAAG TGAGCCTGAG 
GGGGACAACT GGTACCCCAC CAACATGGAG GAGCTGGTCA CAGTGGACGA AGTAGGGGAG 
GAAGACTTCA TCATGGAGCC AGACATACCG GAGCTGGAAG AAATTGTACC CATCGACCAG 
AAAGACAAAA TCCTCCCCGA AATATGTCCC TGTGTAACAG CCACCTTAGG TTTGGACTTG 
GCCAAAGACT TCACCAAGCA GGGAGAGACC CTAGGGAACG GAGACGCAGA ACTCAGCCCG 
AAGCTGCCCG GACAAGTGCC GTCTACTTCC ACAAGCTGTC CCAATGACAC GGACATGGAG 
ATGGCTGGCC TAAATCTGGA TGCTGAGCGG AAGCCAGCTG AAAGCGAGAC AGGCCTCTCA 
CCGGAGGTCT CAAATTGCTA CGAGAAGGAG GCGAGAGGAG CGGAGGGCTC AGATGTGCGT 
CTGGCCCCTG CAGCCCAGCG AATGTCCTCC CCTCAGCCAG CAGATGAGAG GGCCCAGCAG 
TCCAGCCCTT TTCTCGATGA CTGCAAGGCC AGGGGGTCCC CAGAAGATGG GCCTCATGAA 
GTCAGCCCCC TGGAAGAAAA AGCCAGCCCC ACTACTGAAA GCGACCTCCA AAGCCAGGCT 
TGCCAAGAAA ACTCCAGGTA CACGGAGACG AGATCTCTGA ACTCGAGATC ACCAGAATTC 
ACCGAAGCGG AGCTGAAAGA GCCCCTTTCT TTGCCTTCCT GGGAACCAGA GGTGTTCAGT 
GAACTTAGCA TTCCTCTAGG AGTGGAGTTC GTGGTTCCCA GGACTGGCTT TTACTGCAAG 
CTGTGTGGCC TGTTCTACAC AAGCGAGGAG GCGGCCAAAG TGAGCCACTG CCGCAGCACT 
GTACACTACA GGAACTTACA GAAGTACCTG TCCCAGCTGG CAGAGGAGGG ACTGAAGGAG 
ACAGAGGGGG TAGACAGCCC AAGCCCCGAG CGCAGCGGGA TTGGTCCACA CTTGGAAAGG 
AAGAAGCTAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Rbm20 ( NM_001107611.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001101081.2 Rattus norvegicus RNA binding motif protein 20 (Rbm20), mRNA.

Price & Availability↑

CloneID	ORa11400
Clone ID Related Accession (Same CDS sequence)	NM_001107611.2
Accession Version	NM_001107611.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3624bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-27
Organism	Rattus norvegicus(Norway rat)
Product	RNA-binding protein 20
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from EU562301.1. On Aug 15, 2009 this sequence version replaced NM_001107611.1. ##Evidence-Data-START## Transcript exon combination :: EU562301.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2689596, SAMEA2689600 [ECO:0000348] ##Evidence-Data-END##

ATGGTGCTGG CTGCAGCCAT GAGCCAAGAC GCGGATCCCA GCGGTCCGGA GCAACCCGAC 
AGAGATGCCT GCATTGTGCC TGGTGTTCAA GGGCCCCCTG CGCCCCAAGG CCAGCAAGGG 
ATGCAGCCCC TGCCGCCACC GCTACCGCCA CCGCCTCAGC CTCAATCCAG CCTGCCCCAG 
ATCATCCAAA ATGCTGCCAA GCTCCTGGAC AAGAACCCTT TCTCCGTCAG TAGCCAGAAC 
CCTCTGCTCA CTTCGCCAGC CAGCGTCCAG CTGGCCCAGA TACAGGCCCA GCTCACCCTC 
CATCGGCTGA AGATGGCACA GACCGCAGTC ACCAACAACA CTGCAGCCGC CACTGTCCTC 
AACCAAGTCC TCTCCAAAGT GGCCATGTCC CAGCCTCTCT TCAACCAGCT TCGCCATCCG 
TCTGTGCTTG GCACCACACA TGGCCCTACT GGGGTCTCCC AGCATGCTGC CACCGTTCCC 
AGTGCTCACT TTCCCTCAAC TGCAATCGCC TTTTCGCCCC CAAGCCAGGC AGGAGGCCCG 
GGGCCTTCTG TGAGCCTCCC CAGCCAGCCC CCCAATGCTA TGGTAGTGCA TACCTTCAGT 
GGGGTGGTGC CTCAGACCCC TGCCCAGCCA GCAGTCATCC TAAGCATTGG GAAGGCGGGG 
CCGACACCTG CTACTACAGG GTTCTATGAC TATGGCAAAG CCAACCCTGG CCAGGCCTAT 
GGTTCTGAAA CGGAGGGCCA GCCGGGCTTC TTGCCAGCCT CGGCGTCAGC CGCAGCATCA 
GGCGGTGTGA CCTATGAAGG ACACTATAGC CACACAGGGC AGGATGGCCA AGCCACCTTT 
TCTAAAGACT TCTATGGACC CAGTGCCCAA GGGTCACATG CAGCAGGTGG GTTCCCAGCT 
GATCAGGCTG GGAGCATGAA AGGAGACGTC GGTGGGTTGT TGCAAGGTAC CAACAGCCAG 
TGGGAGAGGC CCTCTGGGTT CTCTGGCCAG AACAAGGCCG ATATTACAGC CGGGCCCGGT 
TTGTGGGCTC CACCTGCCAG TCAGCCTTAT GAACTATATG ATCCTGAGGA GCCTACCTCA 
GACAGGGCCC CTCCTGCCTT TGGATCTCGA CTTAACAACA GCAAGCAGGG ATTCAACTGC 
TCCTGCCGGC GGACAAAGGA GGGGCAGGCC ATGCTGTCCG TGAGGCCCCT GCAGGGTCAT 
CAACTGAATG ACTTCCGAGG CTTGGCTCCA CTCCACCTTC CACATATCTG CAGCATCTGT 
GACAAGAAGG TGTTTGACCT GAAGGACTGG GAGCTACATG TGAAGGGGAA GTTGCATGCC 
CAGAAATGCC TGCTCTTCTC AGAAAGTGCT GGCCTCCGGA GTATATGTGC TACAGGAGAA 
GGGACGCTGT CTGCCTCTGC AAACAGCACA GCTGTTTATA ACCCCACTGG AAATGAGGAT 
TATACCTCAA CTCTTGGAAC ATCATATGCA GCCATTCCAA CAAGGGCCTT TGCTCAATCA 
AACCCCATGT TTCCTTCGGC TTCCTCTGGG ACAAATTTTG CACAGAGGAA AGGTGCTGGA 
CGAGTTGTGC ACATCTGCAA TCTCCCAGAG GGCAGCTGCA CGGAGAATGA CGTCATCAAC 
CTGGGGCTGC CCTTTGGCAA GGTCACTAAT TACATCCTTA TGAAGTCAAC TAATCAGGCT 
TTCTTGGAGA TGGCTTACAC AGAAGCTGCT CAAGCTATGG TCCAGTACTA CCAAGAAAAG 
CCCGCGCTTA TCAATGGCGA GAAGTTACTC ATTCGGATGT CCACGAGATA CAAGGAATTG 
CAGCTGAAGA AACCTGGGAA AAATGTGGCT GCTATCATCC AGGACATCCA CTCCCAAAGG 
GAGAGGTGCA TGCTCCGGGA AGCTGACAGA TATGGTCCAG AGCGACCACG TTCTCGAAGT 
CCAATGAGCC GATCGCTCTC CCCGAGATCC CACAGTCCTC CTGGCCCCTC TCGGGCTGAT 
TGGGGCAATG GCCGTGACTC CTACGCATGG AGGGACGAGG ATCGGGAGAC GGTGCCCAGG 
AGGGAGAACG GGGAAGACAA GAGGGACAGG TTGGATGTTT GGGCACATGA CCGGAAACAC 
TATCCCAGGC AGCTGGACAA AGCCGAGTTG GATGAGCGAC TCGAAGGAGG GAGGGGCTAC 
CGGGAAAAGT ACTTGAAGTC AGGGTCCCCC GGCCCACTCC ATTCTGCGTC TGGCTACAAA 
GGCCGGGAAG ATGGCTACCA TCGAAAAGAG ACTAAAGCTA AGTTGGACAA ACACCCAAAG 
CAGCAGCAGC AGGATGTGCC AGGAAGATCC AGGAGGAAAG AAGAGGCGCG ACTACGGGAG 
CCCAGACACC CTCACCCAGA GGACTCTGGC AAGGAAGAGG ATCTGGAGCC CAAGGTCACT 
CGGGCCCCCG AGGGTACCAA GTCCAAGCAA AGTGAGAAAA GTAAAACCAA GAGAGCCGAC 
AGAGACCAAG AAGGAGCTGA TGACAAAAAA GAAGGCCGAG GGGCAGAGAA TGAGGCTGGG 
ACTGAGGAAC AGGAAGGCAT GGAAGAGAGC CCCGCATCGG TGGGCACACA GCAGGAAGGG 
ACGGAGTCCT CCGATCCAGA AAACACAAGG ACAAAGAAGG GACAAGACTG TGACAGTGGA 
AGTGAGCCTG AGGGGGACAA CTGGTACCCC ACCAACATGG AGGAGCTGGT CACAGTGGAC 
GAAGTAGGGG AGGAAGACTT CATCATGGAG CCAGACATAC CGGAGCTGGA AGAAATTGTA 
CCCATCGACC AGAAAGACAA AATCCTCCCC GAAATATGTC CCTGTGTAAC AGCCACCTTA 
GGTTTGGACT TGGCCAAAGA CTTCACCAAG CAGGGAGAGA CCCTAGGGAA CGGAGACGCA 
GAACTCAGCC CGAAGCTGCC CGGACAAGTG CCGTCTACTT CCACAAGCTG TCCCAATGAC 
ACGGACATGG AGATGGCTGG CCTAAATCTG GATGCTGAGC GGAAGCCAGC TGAAAGCGAG 
ACAGGCCTCT CACCGGAGGT CTCAAATTGC TACGAGAAGG AGGCGAGAGG AGCGGAGGGC 
TCAGATGTGC GTCTGGCCCC TGCAGCCCAG CGAATGTCCT CCCCTCAGCC AGCAGATGAG 
AGGGCCCGGC AGTCCAGCCC TTTTCTCGAT GACTGCAAGG CCAGGGGGTC CCCAGAAGAT 
GGGCCTCATG AAGTCAGCCC CCTGGAAGAA AAAGCCAGCC CCACTACTGA AAGCGACCTC 
CAAAGCCAGG CTTGCCAAGA AAACTCCAGG TACACGGAGA CGAGATCTCT GAACTCGAGA 
TCACCAGAAT TCACCGAAGC GGAGCTGAAA GAGCCCCTTT CTTTGCCTTC CTGGGAACCA 
GAGGTGTTCA GTGAACTTAG CATTCCTCTA GGAGTGGAGT TCGTGGTTCC CAGGACTGGC 
TTTTACTGCA AGCTGTGTGG CCTGTTCTAC ACAAGCGAGG AGGCGGCCAA AGTGAGCCAC 
TGCCGCAGCA CTGTACACTA CAGGAACTTA CAGAAGTACC TGTCCCAGCT GGCAGAGGAG 
GGACTGAAGG AGACAGAGGG GGTAGACAGC CCAAGCCCCG AGCGCAGCGG GATTGGTCCA 
CACTTGGAAA GGAAGAAGCT ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001101081.2
CDS	103..3726
Translation	MVLAAAMSQDADPSGPEQPDRDACIVPGVQGPPAPQGQQGMQPLPPPLPPPPQPQSSLPQIIQNAAKLLDKNPFSVSSQNPLLTSPASVQLAQIQAQLTLHRLKMAQTAVTNNTAAATVLNQVLSKVAMSQPLFNQLRHPSVLGTTHGPTGVSQHAATVPSAHFPSTAIAFSPPSQAGGPGPSVSLPSQPPNAMVVHTFSGVVPQTPAQPAVILSIGKAGPTPATTGFYDYGKANPGQAYGSETEGQPGFLPASASAAASGGVTYEGHYSHTGQDGQATFSKDFYGPSAQGSHAAGGFPADQAGSMKGDVGGLLQGTNSQWERPSGFSGQNKADITAGPGLWAPPASQPYELYDPEEPTSDRAPPAFGSRLNNSKQGFNCSCRRTKEGQAMLSVRPLQGHQLNDFRGLAPLHLPHICSICDKKVFDLKDWELHVKGKLHAQKCLLFSESAGLRSICATGEGTLSASANSTAVYNPTGNEDYTSTLGTSYAAIPTRAFAQSNPMFPSASSGTNFAQRKGAGRVVHICNLPEGSCTENDVINLGLPFGKVTNYILMKSTNQAFLEMAYTEAAQAMVQYYQEKPALINGEKLLIRMSTRYKELQLKKPGKNVAAIIQDIHSQRERCMLREADRYGPERPRSRSPMSRSLSPRSHSPPGPSRADWGNGRDSYAWRDEDRETVPRRENGEDKRDRLDVWAHDRKHYPRQLDKAELDERLEGGRGYREKYLKSGSPGPLHSASGYKGREDGYHRKETKAKLDKHPKQQQQDVPGRSRRKEEARLREPRHPHPEDSGKEEDLEPKVTRAPEGTKSKQSEKSKTKRADRDQEGADDKKEGRGAENEAGTEEQEGMEESPASVGTQQEGTESSDPENTRTKKGQDCDSGSEPEGDNWYPTNMEELVTVDEVGEEDFIMEPDIPELEEIVPIDQKDKILPEICPCVTATLGLDLAKDFTKQGETLGNGDAELSPKLPGQVPSTSTSCPNDTDMEMAGLNLDAERKPAESETGLSPEVSNCYEKEARGAEGSDVRLAPAAQRMSSPQPADERARQSSPFLDDCKARGSPEDGPHEVSPLEEKASPTTESDLQSQACQENSRYTETRSLNSRSPEFTEAELKEPLSLPSWEPEVFSELSIPLGVEFVVPRTGFYCKLCGLFYTSEEAAKVSHCRSTVHYRNLQKYLSQLAEEGLKETEGVDSPSPERSGIGPHLERKKL

Target ORF information:

RefSeq Version	NM_001107611.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus RNA binding motif protein 20 (Rbm20), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001107611.2

ORF Insert Sequence:

ATGGTGCTGG CTGCAGCCAT GAGCCAAGAC GCGGATCCCA GCGGTCCGGA GCAACCCGAC 
AGAGATGCCT GCATTGTGCC TGGTGTTCAA GGGCCCCCTG CGCCCCAAGG CCAGCAAGGG 
ATGCAGCCCC TGCCGCCACC GCTACCGCCA CCGCCTCAGC CTCAATCCAG CCTGCCCCAG 
ATCATCCAAA ATGCTGCCAA GCTCCTGGAC AAGAACCCTT TCTCCGTCAG TAGCCAGAAC 
CCTCTGCTCA CTTCGCCAGC CAGCGTCCAG CTGGCCCAGA TACAGGCCCA GCTCACCCTC 
CATCGGCTGA AGATGGCACA GACCGCAGTC ACCAACAACA CTGCAGCCGC CACTGTCCTC 
AACCAAGTCC TCTCCAAAGT GGCCATGTCC CAGCCTCTCT TCAACCAGCT TCGCCATCCG 
TCTGTGCTTG GCACCACACA TGGCCCTACT GGGGTCTCCC AGCATGCTGC CACCGTTCCC 
AGTGCTCACT TTCCCTCAAC TGCAATCGCC TTTTCGCCCC CAAGCCAGGC AGGAGGCCCG 
GGGCCTTCTG TGAGCCTCCC CAGCCAGCCC CCCAATGCTA TGGTAGTGCA TACCTTCAGT 
GGGGTGGTGC CTCAGACCCC TGCCCAGCCA GCAGTCATCC TAAGCATTGG GAAGGCGGGG 
CCGACACCTG CTACTACAGG GTTCTATGAC TATGGCAAAG CCAACCCTGG CCAGGCCTAT 
GGTTCTGAAA CGGAGGGCCA GCCGGGCTTC TTGCCAGCCT CGGCGTCAGC CGCAGCATCA 
GGCGGTGTGA CCTATGAAGG ACACTATAGC CACACAGGGC AGGATGGCCA AGCCACCTTT 
TCTAAAGACT TCTATGGACC CAGTGCCCAA GGGTCACATG CAGCAGGTGG GTTCCCAGCT 
GATCAGGCTG GGAGCATGAA AGGAGACGTC GGTGGGTTGT TGCAAGGTAC CAACAGCCAG 
TGGGAGAGGC CCTCTGGGTT CTCTGGCCAG AACAAGGCCG ATATTACAGC CGGGCCCGGT 
TTGTGGGCTC CACCTGCCAG TCAGCCTTAT GAACTATATG ATCCTGAGGA GCCTACCTCA 
GACAGGGCCC CTCCTGCCTT TGGATCTCGA CTTAACAACA GCAAGCAGGG ATTCAACTGC 
TCCTGCCGGC GGACAAAGGA GGGGCAGGCC ATGCTGTCCG TGAGGCCCCT GCAGGGTCAT 
CAACTGAATG ACTTCCGAGG CTTGGCTCCA CTCCACCTTC CACATATCTG CAGCATCTGT 
GACAAGAAGG TGTTTGACCT GAAGGACTGG GAGCTACATG TGAAGGGGAA GTTGCATGCC 
CAGAAATGCC TGCTCTTCTC AGAAAGTGCT GGCCTCCGGA GTATATGTGC TACAGGAGAA 
GGGACGCTGT CTGCCTCTGC AAACAGCACA GCTGTTTATA ACCCCACTGG AAATGAGGAT 
TATACCTCAA CTCTTGGAAC ATCATATGCA GCCATTCCAA CAAGGGCCTT TGCTCAATCA 
AACCCCATGT TTCCTTCGGC TTCCTCTGGG ACAAATTTTG CACAGAGGAA AGGTGCTGGA 
CGAGTTGTGC ACATCTGCAA TCTCCCAGAG GGCAGCTGCA CGGAGAATGA CGTCATCAAC 
CTGGGGCTGC CCTTTGGCAA GGTCACTAAT TACATCCTTA TGAAGTCAAC TAATCAGGCT 
TTCTTGGAGA TGGCTTACAC AGAAGCTGCT CAAGCTATGG TCCAGTACTA CCAAGAAAAG 
CCCGCGCTTA TCAATGGCGA GAAGTTACTC ATTCGGATGT CCACGAGATA CAAGGAATTG 
CAGCTGAAGA AACCTGGGAA AAATGTGGCT GCTATCATCC AGGACATCCA CTCCCAAAGG 
GAGAGGTGCA TGCTCCGGGA AGCTGACAGA TATGGTCCAG AGCGACCACG TTCTCGAAGT 
CCAATGAGCC GATCGCTCTC CCCGAGATCC CACAGTCCTC CTGGCCCCTC TCGGGCTGAT 
TGGGGCAATG GCCGTGACTC CTACGCATGG AGGGACGAGG ATCGGGAGAC GGTGCCCAGG 
AGGGAGAACG GGGAAGACAA GAGGGACAGG TTGGATGTTT GGGCACATGA CCGGAAACAC 
TATCCCAGGC AGCTGGACAA AGCCGAGTTG GATGAGCGAC TCGAAGGAGG GAGGGGCTAC 
CGGGAAAAGT ACTTGAAGTC AGGGTCCCCC GGCCCACTCC ATTCTGCGTC TGGCTACAAA 
GGCCGGGAAG ATGGCTACCA TCGAAAAGAG ACTAAAGCTA AGTTGGACAA ACACCCAAAG 
CAGCAGCAGC AGGATGTGCC AGGAAGATCC AGGAGGAAAG AAGAGGCGCG ACTACGGGAG 
CCCAGACACC CTCACCCAGA GGACTCTGGC AAGGAAGAGG ATCTGGAGCC CAAGGTCACT 
CGGGCCCCCG AGGGTACCAA GTCCAAGCAA AGTGAGAAAA GTAAAACCAA GAGAGCCGAC 
AGAGACCAAG AAGGAGCTGA TGACAAAAAA GAAGGCCGAG GGGCAGAGAA TGAGGCTGGG 
ACTGAGGAAC AGGAAGGCAT GGAAGAGAGC CCCGCATCGG TGGGCACACA GCAGGAAGGG 
ACGGAGTCCT CCGATCCAGA AAACACAAGG ACAAAGAAGG GACAAGACTG TGACAGTGGA 
AGTGAGCCTG AGGGGGACAA CTGGTACCCC ACCAACATGG AGGAGCTGGT CACAGTGGAC 
GAAGTAGGGG AGGAAGACTT CATCATGGAG CCAGACATAC CGGAGCTGGA AGAAATTGTA 
CCCATCGACC AGAAAGACAA AATCCTCCCC GAAATATGTC CCTGTGTAAC AGCCACCTTA 
GGTTTGGACT TGGCCAAAGA CTTCACCAAG CAGGGAGAGA CCCTAGGGAA CGGAGACGCA 
GAACTCAGCC CGAAGCTGCC CGGACAAGTG CCGTCTACTT CCACAAGCTG TCCCAATGAC 
ACGGACATGG AGATGGCTGG CCTAAATCTG GATGCTGAGC GGAAGCCAGC TGAAAGCGAG 
ACAGGCCTCT CACCGGAGGT CTCAAATTGC TACGAGAAGG AGGCGAGAGG AGCGGAGGGC 
TCAGATGTGC GTCTGGCCCC TGCAGCCCAG CGAATGTCCT CCCCTCAGCC AGCAGATGAG 
AGGGCCCGGC AGTCCAGCCC TTTTCTCGAT GACTGCAAGG CCAGGGGGTC CCCAGAAGAT 
GGGCCTCATG AAGTCAGCCC CCTGGAAGAA AAAGCCAGCC CCACTACTGA AAGCGACCTC 
CAAAGCCAGG CTTGCCAAGA AAACTCCAGG TACACGGAGA CGAGATCTCT GAACTCGAGA 
TCACCAGAAT TCACCGAAGC GGAGCTGAAA GAGCCCCTTT CTTTGCCTTC CTGGGAACCA 
GAGGTGTTCA GTGAACTTAG CATTCCTCTA GGAGTGGAGT TCGTGGTTCC CAGGACTGGC 
TTTTACTGCA AGCTGTGTGG CCTGTTCTAC ACAAGCGAGG AGGCGGCCAA AGTGAGCCAC 
TGCCGCAGCA CTGTACACTA CAGGAACTTA CAGAAGTACC TGTCCCAGCT GGCAGAGGAG 
GGACTGAAGG AGACAGAGGG GGTAGACAGC CCAAGCCCCG AGCGCAGCGG GATTGGTCCA 
CACTTGGAAA GGAAGAAGCT ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Insulin regulates titin pre-mRNA splicing through the PI3K-Akt-mTOR kinase axis in a RBM20-dependent manner.
Biochimica et biophysica acta1863(9)2363-2371(2017 Sep)
Zhu C,Yin Z,Tan B,Guo W

RBM20 and RBM24 cooperatively promote the expression of short enh splice variants.
FEBS letters590(14)2262-74(2016 07)
Ito J,Iijima M,Yoshimoto N,Niimi T,Kuroda S,Maturana AD

RNA-binding protein RBM20 represses splicing to orchestrate cardiac pre-mRNA processing.
The Journal of clinical investigation124(8)3419-30(2014 Aug)
Maatz H,Jens M,Liss M,Schafer S,Heinig M,Kirchner M,Adami E,Rintisch C,Dauksaite V,Radke MH,Selbach M,Barton PJ,Cook SA,Rajewsky N,Gotthardt M,Landthaler M,Hubner N

Pathophysiological defects and transcriptional profiling in the RBM20-/- rat model.
PloS one8(12)e84281(2013)
Guo W,Pleitner JM,Saupe KW,Greaser ML

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Rbm20 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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