Chuk cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Chuk
Entrez Gene ID	309361
Full Name	conserved helix-loop-helix ubiquitous kinase
Synonyms	Ikbka,Ikka
General protein information	Preferred Names conserved helix-loop-helix ubiquitous kinase Names inhibitor of nuclear factor kappa-B kinase subunit alpha
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 1 Map Location: 1q54

mRNA and Protein(s)

mRNA	Protein	Name
XM_008760418.2	XP_008758640.1	inhibitor of nuclear factor kappa-B kinase subunit alpha isoform X1
XM_006231481.2	XP_006231543.1	inhibitor of nuclear factor kappa-B kinase subunit alpha isoform X2
NM_001107588.1	NP_001101058.1	inhibitor of nuclear factor kappa-B kinase subunit alpha
XM_008760419.2	XP_008758641.1	inhibitor of nuclear factor kappa-B kinase subunit alpha isoform X1
XM_017589269.1	XP_017444758.1	inhibitor of nuclear factor kappa-B kinase subunit alpha isoform X3

Pathways from BioSystems

Homologies

Homo sapiens (human)	CHUK	NP_001269.3
	CHUK	XP_001107171.1
Canis lupus familiaris (dog)	CHUK	XP_534990.2
Gallus gallus (chicken)	CHUK	NP_001012922.1
Bos taurus (cattle)	CHUK	NP_776446.1
Danio rerio (zebrafish)	chuk	NP_956611.1
Xenopus tropicalis (tropical clawed frog)	chuk	NP_001016900.1
Pan troglodytes (chimpanzee)	CHUK	XP_001167720.1
Mus musculus (house mouse)	Chuk	NP_031726.2
Rattus norvegicus (Norway rat)	Chuk	NP_001101058.1

Gene Ontology
Bibliography

Related articles in PubMed

Cadmium induces matrix metalloproteinase-9 expression via ROS-dependent EGFR, NF-?B, and AP-1 pathways in human endothelial cells.
Lian S, Xia Y, Khoi PN, Ung TT, Yoon HJ, Kim NH, Kim KK, Jung YD
Toxicology338104-16(2015 Dec)

Apigenin blocks IKK? activation and suppresses prostate cancer progression.
Shukla S, Kanwal R, Shankar E, Datt M, Chance MR, Fu P, MacLennan GT, Gupta S
Oncotarget6(31)31216-32(2015 Oct)

Suppression of NF-?B and NF-?B-Regulated Gene Expression by Apigenin through I?B? and IKK Pathway in TRAMP Mice.
Shukla S, Shankar E, Fu P, MacLennan GT, Gupta S
PloS one10(9)e0138710(2015)

IKK regulates the deubiquitinase CYLD at the postsynaptic density.
Thein S, Pham A, Bayer KU, Tao-Cheng JH, Dosemeci A
Biochemical and biophysical research communications450(1)550-4(2014 Jul)

A new transcriptional role for matrix metalloproteinase-12 in antiviral immunity.
Marchant DJ, Bellac CL, Moraes TJ, Wadsworth SJ, Dufour A, Butler GS, Bilawchuk LM, Hendry RG, Robertson AG, Cheung CT, Ng J, Ang L, Luo Z, Heilbron K, Norris MJ, Duan W, Bucyk T, Karpov A, Devel L, Georgiadis D, Hegele RG, Luo H, Granville DJ, Dive V, McManus BM, Overall CM
Nature medicine20(5)493-502(2014 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Altogether, the results suggest that in the absence of excitatory conditions, constitutive IKK activity at the PSD regulates CYLD and maintains basal levels of K63-linkage specific deubiquitination at the synapse.
Title: IKK regulates the deubiquitinase CYLD at the postsynaptic density.

Increased IKKalpha/beta and JNK serine phosphorylation may contribute to increasing IRS-1 serine phosphorylation, while concurrent co-localization of SOCS-3 with both IR-beta and IRS-1 may prevent IRS-1 from interacting with IR-beta; these 2 mechanisms may independently contribute to impairing insulin-stimulated PI3-K activation in the skeletal muscle of the obese Zucker rat.
Title: Insulin receptor and IRS-1 co-immunoprecipitation with SOCS-3, and IKK?/? phosphorylation are increased in obese Zucker rat skeletal muscle.

High-fat feeding increases IKKalpha phosphorylation in skeletal muscle.
Title: High-fat feeding increases insulin receptor and IRS-1 coimmunoprecipitation with SOCS-3, IKKalpha/beta phosphorylation and decreases PI-3 kinase activity in muscle.

both IKKalpha and IKKbeta are necessary and sufficient for physiological muscle atrophy.
Title: The IkappaB kinases IKKalpha and IKKbeta are necessary and sufficient for skeletal muscle atrophy.

canonical IKKbeta-dependent NF-kappaB activation is inhibited by peroxynitrite, which also activates NF-kappaB-inducing kinase and IKKalpha phosphorylation
Title: Peroxynitrite is a potent inhibitor of NF-{kappa}B activation triggered by inflammatory stimuli in cardiac and endothelial cell lines.

The following Chuk gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Chuk cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa04124	NM_001107588.1 Latest version!	Rattus norvegicus component of inhibitor of nuclear factor kappa B kinase complex (Chuk), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$440.30 $629.00
ORa24152	XM_006231481.2 Latest version!	Rattus norvegicus conserved helix-loop-helix ubiquitous kinase (Chuk), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
ORa46732	XM_008760419.2 Latest version!	Rattus norvegicus conserved helix-loop-helix ubiquitous kinase (Chuk), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
ORa46732	XM_008760418.2 Latest version!	Rattus norvegicus conserved helix-loop-helix ubiquitous kinase (Chuk), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
ORa56642	XM_017589269.1 Latest version!	Rattus norvegicus conserved helix-loop-helix ubiquitous kinase (Chuk), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Chuk ( NM_001107588.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001101058.1 Rattus norvegicus component of inhibitor of nuclear factor kappa B kinase complex (Chuk), mRNA.

Price & Availability↑

CloneID	ORa04124
Clone ID Related Accession (Same CDS sequence)	NM_001107588.1
Accession Version	NM_001107588.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2238bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-15
Organism	Rattus norvegicus(Norway rat)
Product	inhibitor of nuclear factor kappa-B kinase subunit alpha
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CH473986.1. On or before Oct 4, 2007 this sequence version replaced XM_219857.4, XM_001061709.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGGAGCGGC CCCCGGGGCT GCGGCCTGGC GCCGGCGGCC CCTGGGAGAT GCGGGAGAGG 
CTTGGCACCG GCGGCTTCGG GAACGTCAGT CTGTACCAGC ACCGGGAACT TGATCTCAAA 
ATAGCAATTA AGTCTTGTCG CTTAGAGCTA AGTACCAAAA ACAGAGAACG ATGGTGCCAT 
GAAATCCAGA TCATGAAAAA GTTGAACCAT GCAAATGTTG TAAAGGCCTG TGATGTTCCT 
GAGGAATTGA ATTTTTTAAT TAACGATGTG CCTCTTCTAG CAATGGAGTA CTGTTCTGGA 
GGGGACCTCC GGAAGCTACT CAACAAACCA GAAAATTGTT GTGGACTTAA AGAGAGCCAG 
ATCCTTTCTT TACTGAGTGA CATAGGATCT GGGATTCGTT ATTTGCATGA AAACAAAATT 
ATACATCGAG ATCTAAAACC TGAAAATATA GTGCTTCAAG ATGTTGGTGG GAAGATAATG 
CATAAAATAA TTGATTTGGG TTATGCCAAA GATGTCGATC AAGGAAGTCT CTGTACATCT 
TTTGTGGGAA CATTACAGTA TTTGGCCCCA GAGCTCTTTG AAAATAAGCC ATACACAGCC 
ACTGTGGATT ATTGGAGCTT TGGGACCATG GTGTTTGAAT GTATTGCTGG ATATAGGCCT 
TTTTTGCATC ATCTGCAGCC ATTTACCTGG CATGAAAAGA TTAAGAAGAA AGATCCAAAG 
TGTATATTTG CTTGTGAAGA GATGACCGGA GAAGTTCGGT TTAGTAGCCA TTTACCTCAG 
CCAAACAACC TTTGTAGTTT AATAGTAGAG CCAATGGAAA GCTGGCTACA GTTGATGCTG 
AATTGGGACC CACAGCAGAG AGGGGGACCT ATTGACCCTC CTTTGAAGCA GCCAAAATGC 
TTTGTGTTAA TGGATAACAT CCTCAACTTG AAGATAGTGC ACATCCTAAA TATGACTTCT 
GCAAAAATCA TTTCTTTTCT GTTACCATAT GATGAAAGTC TCCATTCACT ACAGTCTCGA 
ATTGAGCGTG AAACAGGAAT AAATACAGGT TCTCAAGAAC TTCTGTCAGA GACAGGAATT 
TCTCTGGATC CTCGGAAACC AGCCTCTCAA TGTGTTCTAG ATGGAGTGAG AGGCTGTGAT 
AGCTACATGG TTTATTTGTT TGATAAAAGT AAGACTGTAT ATGAAGGACC ATTTGCATCC 
AGAAGTTTAT CTGATTGTGT AAATTATATT GTGCAAGACA GCAAGATACA GCTTCCAGTT 
ACACAGCTTC GCAAAGTGTG GGCTGAAGCA GTGCACTATG TATCTGGGCT AAAGGAGGAC 
TACAGCAGGC TCTTTCAGGG ACAAAGAGCA GCAATGTTAA GTCTTCTTAG ATATAATGCT 
AACTTGACAA AAATGAAGAA TACTTTGATC TCAGCATCAC AGCAACTCAA AGCTAAATTG 
GAGTTTTTTC GCAAAAGCAT TCAGCTTGAC TTGGAGAGAT ATAGTGAGCA GATGACTTAT 
GGGATATCTT CAGAAAAAAT GCTGAAAGCA TGGAAAGAAA TGGAAGAAAA GGCCATTCAC 
TATTCTGAGG TTGGTGTCAT TGGATACCTT GAGGATCAAA TTATGTCTTT GCACACTGAA 
ATCATGGAGC TGCAGAAGAG CCCCTATGGG AGACGCCAGG GAGACCTGAT GGAATCCCTG 
GAACAGCGTG CCATTGATCT CTATAAGCAG CTAAAACACA GACCCACCGA TCACTCCTAC 
AGTGACAGCA CGGAGATGGT GAAGATCATT GTGCACACTG TGCAGAGTCA GGACCGAGTT 
CTCAAGGAGC TGTTTGGTCA CCTGAGCAAG TTGTTGGGCT GCAAGCAGAA GATTATTGAT 
CTACTCCCCA AGGTGGAAGT GGCCCTCAGT AACATCAAAG AAGCTGACAA TACTGTCATG 
TTTATGCAGG GAAAGAGGCA GAAAGAAATT TGGCACCTCC TTAAAATTGC TTGTACACAG 
AGTTCTGCTC GCTCTCTTGT AGGATCCAGT CTAGAAGGCA CAGTAACCCC TCCAGTATCA 
GCATGGCTGC CCCCTACTCT AGCAGACCGT GAACATCCTC TGACATGTGT GGTAACTCCT 
CAAGATGGCG AGACGTTAGC ACAAATGATA GAAGAAAATC TGAACTGTCT TGGCCATTTA 
AGTACTATTA TTCGTGAAGC AAATGAGGAC CAGAGCAGTA GTATGATGAG TCTTGATTGG 
AGTTGGTTAG CAGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001101058.1
CDS	99..2336
Translation	MERPPGLRPGAGGPWEMRERLGTGGFGNVSLYQHRELDLKIAIKSCRLELSTKNRERWCHEIQIMKKLNHANVVKACDVPEELNFLINDVPLLAMEYCSGGDLRKLLNKPENCCGLKESQILSLLSDIGSGIRYLHENKIIHRDLKPENIVLQDVGGKIMHKIIDLGYAKDVDQGSLCTSFVGTLQYLAPELFENKPYTATVDYWSFGTMVFECIAGYRPFLHHLQPFTWHEKIKKKDPKCIFACEEMTGEVRFSSHLPQPNNLCSLIVEPMESWLQLMLNWDPQQRGGPIDPPLKQPKCFVLMDNILNLKIVHILNMTSAKIISFLLPYDESLHSLQSRIERETGINTGSQELLSETGISLDPRKPASQCVLDGVRGCDSYMVYLFDKSKTVYEGPFASRSLSDCVNYIVQDSKIQLPVTQLRKVWAEAVHYVSGLKEDYSRLFQGQRAAMLSLLRYNANLTKMKNTLISASQQLKAKLEFFRKSIQLDLERYSEQMTYGISSEKMLKAWKEMEEKAIHYSEVGVIGYLEDQIMSLHTEIMELQKSPYGRRQGDLMESLEQRAIDLYKQLKHRPTDHSYSDSTEMVKIIVHTVQSQDRVLKELFGHLSKLLGCKQKIIDLLPKVEVALSNIKEADNTVMFMQGKRQKEIWHLLKIACTQSSARSLVGSSLEGTVTPPVSAWLPPTLADREHPLTCVVTPQDGETLAQMIEENLNCLGHLSTIIREANEDQSSSMMSLDWSWLAE

Target ORF information:

RefSeq Version	NM_001107588.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus component of inhibitor of nuclear factor kappa B kinase complex (Chuk), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001107588.1

ORF Insert Sequence:

ATGGAGCGGC CCCCGGGGCT GCGGCCTGGC GCCGGCGGCC CCTGGGAGAT GCGGGAGAGG 
CTTGGCACCG GCGGCTTCGG GAACGTCAGT CTGTACCAGC ACCGGGAACT TGATCTCAAA 
ATAGCAATTA AGTCTTGTCG CTTAGAGCTA AGTACCAAAA ACAGAGAACG ATGGTGCCAT 
GAAATCCAGA TCATGAAAAA GTTGAACCAT GCAAATGTTG TAAAGGCCTG TGATGTTCCT 
GAGGAATTGA ATTTTTTAAT TAACGATGTG CCTCTTCTAG CAATGGAGTA CTGTTCTGGA 
GGGGACCTCC GGAAGCTACT CAACAAACCA GAAAATTGTT GTGGACTTAA AGAGAGCCAG 
ATCCTTTCTT TACTGAGTGA CATAGGATCT GGGATTCGTT ATTTGCATGA AAACAAAATT 
ATACATCGAG ATCTAAAACC TGAAAATATA GTGCTTCAAG ATGTTGGTGG GAAGATAATG 
CATAAAATAA TTGATTTGGG TTATGCCAAA GATGTCGATC AAGGAAGTCT CTGTACATCT 
TTTGTGGGAA CATTACAGTA TTTGGCCCCA GAGCTCTTTG AAAATAAGCC ATACACAGCC 
ACTGTGGATT ATTGGAGCTT TGGGACCATG GTGTTTGAAT GTATTGCTGG ATATAGGCCT 
TTTTTGCATC ATCTGCAGCC ATTTACCTGG CATGAAAAGA TTAAGAAGAA AGATCCAAAG 
TGTATATTTG CTTGTGAAGA GATGACCGGA GAAGTTCGGT TTAGTAGCCA TTTACCTCAG 
CCAAACAACC TTTGTAGTTT AATAGTAGAG CCAATGGAAA GCTGGCTACA GTTGATGCTG 
AATTGGGACC CACAGCAGAG AGGGGGACCT ATTGACCCTC CTTTGAAGCA GCCAAAATGC 
TTTGTGTTAA TGGATAACAT CCTCAACTTG AAGATAGTGC ACATCCTAAA TATGACTTCT 
GCAAAAATCA TTTCTTTTCT GTTACCATAT GATGAAAGTC TCCATTCACT ACAGTCTCGA 
ATTGAGCGTG AAACAGGAAT AAATACAGGT TCTCAAGAAC TTCTGTCAGA GACAGGAATT 
TCTCTGGATC CTCGGAAACC AGCCTCTCAA TGTGTTCTAG ATGGAGTGAG AGGCTGTGAT 
AGCTACATGG TTTATTTGTT TGATAAAAGT AAGACTGTAT ATGAAGGACC ATTTGCATCC 
AGAAGTTTAT CTGATTGTGT AAATTATATT GTGCAAGACA GCAAGATACA GCTTCCAGTT 
ACACAGCTTC GCAAAGTGTG GGCTGAAGCA GTGCACTATG TATCTGGGCT AAAGGAGGAC 
TACAGCAGGC TCTTTCAGGG ACAAAGAGCA GCAATGTTAA GTCTTCTTAG ATATAATGCT 
AACTTGACAA AAATGAAGAA TACTTTGATC TCAGCATCAC AGCAACTCAA AGCTAAATTG 
GAGTTTTTTC GCAAAAGCAT TCAGCTTGAC TTGGAGAGAT ATAGTGAGCA GATGACTTAT 
GGGATATCTT CAGAAAAAAT GCTGAAAGCA TGGAAAGAAA TGGAAGAAAA GGCCATTCAC 
TATTCTGAGG TTGGTGTCAT TGGATACCTT GAGGATCAAA TTATGTCTTT GCACACTGAA 
ATCATGGAGC TGCAGAAGAG CCCCTATGGG AGACGCCAGG GAGACCTGAT GGAATCCCTG 
GAACAGCGTG CCATTGATCT CTATAAGCAG CTAAAACACA GACCCACCGA TCACTCCTAC 
AGTGACAGCA CGGAGATGGT GAAGATCATT GTGCACACTG TGCAGAGTCA GGACCGAGTT 
CTCAAGGAGC TGTTTGGTCA CCTGAGCAAG TTGTTGGGCT GCAAGCAGAA GATTATTGAT 
CTACTCCCCA AGGTGGAAGT GGCCCTCAGT AACATCAAAG AAGCTGACAA TACTGTCATG 
TTTATGCAGG GAAAGAGGCA GAAAGAAATT TGGCACCTCC TTAAAATTGC TTGTACACAG 
AGTTCTGCTC GCTCTCTTGT AGGATCCAGT CTAGAAGGCA CAGTAACCCC TCCAGTATCA 
GCATGGCTGC CCCCTACTCT AGCAGACCGT GAACATCCTC TGACATGTGT GGTAACTCCT 
CAAGATGGCG AGACGTTAGC ACAAATGATA GAAGAAAATC TGAACTGTCT TGGCCATTTA 
AGTACTATTA TTCGTGAAGC AAATGAGGAC CAGAGCAGTA GTATGATGAG TCTTGATTGG 
AGTTGGTTAG CAGAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Chuk ( XM_006231481.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006231543.1 Rattus norvegicus conserved helix-loop-helix ubiquitous kinase (Chuk), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ORa24152
Clone ID Related Accession (Same CDS sequence)	XM_006231481.2
Accession Version	XM_006231481.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2160bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	inhibitor of nuclear factor kappa-B kinase subunit alpha isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 7, 2014 this sequence version replaced XM_006231481.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCGGC CCCCGGGGCT GCGGCCTGGC GCCGGCGGCC CCTGGGAGAT GCGGGAGAGG 
CTTGGCACCG GCGGCTTCGG GAACGTCAGT CTGTACCAGC ACCGGGAACT TGATCTCAAA 
ATAGCAATTA AGTCTTGTCG CTTAGAGCTA AGTACCAAAA ACAGAGAACG ATGGTGCCAT 
GAAATCCAGA TCATGAAAAA GTTGAACCAT GCAAATGTTG TAAAGGCCTG TGATGTTCCT 
GAGGAATTGA ATTTTTTAAT TAACGATGTG CCTCTTCTAG CAATGGAGTA CTGTTCTGGA 
GGGGACCTCC GGAAGCTACT CAACAAACCA GAAAATTGTT GTGGACTTAA AGAGAGCCAG 
ATCCTTTCTT TACTGAGTGA CATAGGATCT GGGATTCGTT ATTTGCATGA AAACAAAATT 
ATACATCGAG ATCTAAAACC TGAAAATATA GTGCTTCAAG ATGTTGGTGG GAAGATAATG 
CATAAAATAA TTGATTTGGG TTATGCCAAA GATGTCGATC AAGGAAGTCT CTGTACATCT 
TTTGTGGGAA CATTACAGTA TTTGGCCCCA GAGCTCTTTG AAAATAAGCC ATACACAGCC 
ACTGTGGATT ATTGGAGCTT TGGGACCATG GTGTTTGAAT GTATTGCTGG ATATAGGCCT 
TTTTTGCATC ATCTGCAGCC ATTTACCTGG CATGAAAAGA TTAAGAAGAA AGATCCAAAG 
TGTATATTTG CTTGTGAAGA GATGACCGGA GAAGTTCGGT TTAGTAGCCA TTTACCTCAG 
CCAAACAACC TTTGTAGTTT AATAGTAGAG CCAATGGAAA GCTGGCTACA GTTGATGCTG 
AATTGGGACC CACAGCAGAG AGGGGGACCT ATTGACCCTC CTTTGAAGCA GCCAAAATGC 
TTTGTGTTAA TGGATAACAT CCTCAACTTG AAGATAGTGC ACATCCTAAA TATGACTTCT 
GCAAAAATCA TTTCTTTTCT GTTACCATAT GATGAAAGTC TCCATTCACT ACAGTCTCGA 
ATTGAGCGTG AAACAGGAAT AAATACAGGT TCTCAAGAAC TTCTGTCAGA GACAGGAATT 
TCTCTGGATC CTCGGAAACC AGCCTCTCAA TGTGTTCTAG ATGGAGTGAG AGGCTGTGAT 
AGCTACATGG TTTATTTGTT TGATAAAAGT AAGACTGTAT ATGAAGGACC ATTTGCATCC 
AGAAGTTTAT CTGATTGTGT AAATTATATT GTGCAAGACA GCAAGATACA GCTTCCAGTT 
ACACAGCTTC GCAAAGTGTG GGCTGAAGCA GTGCACTATG TATCTGGGCT AAAGGAGGAC 
TACAGCAGGC TCTTTCAGGG ACAAAGAGCA GCAATGTTAA GTCTTCTTAG ATATAATGCT 
AACTTGACAA AAATGAAGAA TACTTTGATC TCAGCATCAC AGCAACTCAA AGCTAAATTG 
GAGTTTTTTC GCAAAAGCAT TCAGCTTGAC TTGGAGAGAT ATAGTGAGCA GATGACTTAT 
GGGATATCTT CAGAAAAAAT GCTGAAAGCA TGGAAAGAAA TGGAAGAAAA GGCCATTCAC 
TATTCTGAGG TTGGTGTCAT TGGATACCTT GAGGATCAAA TTATGTCTTT GCACACTGAA 
ATCATGGAGC TGCAGAAGAG CCCCTATGGG AGACGCCAGG GAGACCTGAT GGAATCCCTG 
GAACAGCGTG CCATTGATCT CTATAAGCAG CTAAAACACA GACCCACCGA TCACTCCTAC 
AGTGACAGCA CGGAGATGGT GAAGATCATT GTGCACACTG TGCAGAGTCA GGACCGAGTT 
CTCAAGGAGC TGTTTGGTCA CCTGAGCAAG TTGTTGGGCT GCAAGCAGAA GATTATTGAT 
CTACTCCCCA AGGTGGAAGT GGCCCTCAGT AACATCAAAG AAGCTGACAA TACTGTCATG 
TTTATGCAGG GAAAGAGGCA GAAAGAAATT TGGCACCTCC TTAAAATTGC TTGTACACAG 
AGTTCTGCTC GCTCTCTTGT AGGATCCAGT CTAGAAGGCA CAGTAACCCC TCCAGTATCA 
GCATGGCTGC CCCCTACTCT AGCAGACCGT GAACATCCTC TGACATGTGT GCGAGACGTT 
AGCACAAATG ATAGAAGAAA ATCTGAACTG TCTTGGCCAT TTAAGTACTA TTATTCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006231543.1
CDS	96..2255
Translation	MERPPGLRPGAGGPWEMRERLGTGGFGNVSLYQHRELDLKIAIKSCRLELSTKNRERWCHEIQIMKKLNHANVVKACDVPEELNFLINDVPLLAMEYCSGGDLRKLLNKPENCCGLKESQILSLLSDIGSGIRYLHENKIIHRDLKPENIVLQDVGGKIMHKIIDLGYAKDVDQGSLCTSFVGTLQYLAPELFENKPYTATVDYWSFGTMVFECIAGYRPFLHHLQPFTWHEKIKKKDPKCIFACEEMTGEVRFSSHLPQPNNLCSLIVEPMESWLQLMLNWDPQQRGGPIDPPLKQPKCFVLMDNILNLKIVHILNMTSAKIISFLLPYDESLHSLQSRIERETGINTGSQELLSETGISLDPRKPASQCVLDGVRGCDSYMVYLFDKSKTVYEGPFASRSLSDCVNYIVQDSKIQLPVTQLRKVWAEAVHYVSGLKEDYSRLFQGQRAAMLSLLRYNANLTKMKNTLISASQQLKAKLEFFRKSIQLDLERYSEQMTYGISSEKMLKAWKEMEEKAIHYSEVGVIGYLEDQIMSLHTEIMELQKSPYGRRQGDLMESLEQRAIDLYKQLKHRPTDHSYSDSTEMVKIIVHTVQSQDRVLKELFGHLSKLLGCKQKIIDLLPKVEVALSNIKEADNTVMFMQGKRQKEIWHLLKIACTQSSARSLVGSSLEGTVTPPVSAWLPPTLADREHPLTCVRDVSTNDRRKSELSWPFKYYYS

Target ORF information:

RefSeq Version	XM_006231481.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus conserved helix-loop-helix ubiquitous kinase (Chuk), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006231481.2

ORF Insert Sequence:

ATGGAGCGGC CCCCGGGGCT GCGGCCTGGC GCCGGCGGCC CCTGGGAGAT GCGGGAGAGG 
CTTGGCACCG GCGGCTTCGG GAACGTCAGT CTGTACCAGC ACCGGGAACT TGATCTCAAA 
ATAGCAATTA AGTCTTGTCG CTTAGAGCTA AGTACCAAAA ACAGAGAACG ATGGTGCCAT 
GAAATCCAGA TCATGAAAAA GTTGAACCAT GCAAATGTTG TAAAGGCCTG TGATGTTCCT 
GAGGAATTGA ATTTTTTAAT TAACGATGTG CCTCTTCTAG CAATGGAGTA CTGTTCTGGA 
GGGGACCTCC GGAAGCTACT CAACAAACCA GAAAATTGTT GTGGACTTAA AGAGAGCCAG 
ATCCTTTCTT TACTGAGTGA CATAGGATCT GGGATTCGTT ATTTGCATGA AAACAAAATT 
ATACATCGAG ATCTAAAACC TGAAAATATA GTGCTTCAAG ATGTTGGTGG GAAGATAATG 
CATAAAATAA TTGATTTGGG TTATGCCAAA GATGTCGATC AAGGAAGTCT CTGTACATCT 
TTTGTGGGAA CATTACAGTA TTTGGCCCCA GAGCTCTTTG AAAATAAGCC ATACACAGCC 
ACTGTGGATT ATTGGAGCTT TGGGACCATG GTGTTTGAAT GTATTGCTGG ATATAGGCCT 
TTTTTGCATC ATCTGCAGCC ATTTACCTGG CATGAAAAGA TTAAGAAGAA AGATCCAAAG 
TGTATATTTG CTTGTGAAGA GATGACCGGA GAAGTTCGGT TTAGTAGCCA TTTACCTCAG 
CCAAACAACC TTTGTAGTTT AATAGTAGAG CCAATGGAAA GCTGGCTACA GTTGATGCTG 
AATTGGGACC CACAGCAGAG AGGGGGACCT ATTGACCCTC CTTTGAAGCA GCCAAAATGC 
TTTGTGTTAA TGGATAACAT CCTCAACTTG AAGATAGTGC ACATCCTAAA TATGACTTCT 
GCAAAAATCA TTTCTTTTCT GTTACCATAT GATGAAAGTC TCCATTCACT ACAGTCTCGA 
ATTGAGCGTG AAACAGGAAT AAATACAGGT TCTCAAGAAC TTCTGTCAGA GACAGGAATT 
TCTCTGGATC CTCGGAAACC AGCCTCTCAA TGTGTTCTAG ATGGAGTGAG AGGCTGTGAT 
AGCTACATGG TTTATTTGTT TGATAAAAGT AAGACTGTAT ATGAAGGACC ATTTGCATCC 
AGAAGTTTAT CTGATTGTGT AAATTATATT GTGCAAGACA GCAAGATACA GCTTCCAGTT 
ACACAGCTTC GCAAAGTGTG GGCTGAAGCA GTGCACTATG TATCTGGGCT AAAGGAGGAC 
TACAGCAGGC TCTTTCAGGG ACAAAGAGCA GCAATGTTAA GTCTTCTTAG ATATAATGCT 
AACTTGACAA AAATGAAGAA TACTTTGATC TCAGCATCAC AGCAACTCAA AGCTAAATTG 
GAGTTTTTTC GCAAAAGCAT TCAGCTTGAC TTGGAGAGAT ATAGTGAGCA GATGACTTAT 
GGGATATCTT CAGAAAAAAT GCTGAAAGCA TGGAAAGAAA TGGAAGAAAA GGCCATTCAC 
TATTCTGAGG TTGGTGTCAT TGGATACCTT GAGGATCAAA TTATGTCTTT GCACACTGAA 
ATCATGGAGC TGCAGAAGAG CCCCTATGGG AGACGCCAGG GAGACCTGAT GGAATCCCTG 
GAACAGCGTG CCATTGATCT CTATAAGCAG CTAAAACACA GACCCACCGA TCACTCCTAC 
AGTGACAGCA CGGAGATGGT GAAGATCATT GTGCACACTG TGCAGAGTCA GGACCGAGTT 
CTCAAGGAGC TGTTTGGTCA CCTGAGCAAG TTGTTGGGCT GCAAGCAGAA GATTATTGAT 
CTACTCCCCA AGGTGGAAGT GGCCCTCAGT AACATCAAAG AAGCTGACAA TACTGTCATG 
TTTATGCAGG GAAAGAGGCA GAAAGAAATT TGGCACCTCC TTAAAATTGC TTGTACACAG 
AGTTCTGCTC GCTCTCTTGT AGGATCCAGT CTAGAAGGCA CAGTAACCCC TCCAGTATCA 
GCATGGCTGC CCCCTACTCT AGCAGACCGT GAACATCCTC TGACATGTGT GCGAGACGTT 
AGCACAAATG ATAGAAGAAA ATCTGAACTG TCTTGGCCAT TTAAGTACTA TTATTCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Chuk ( XM_008760418.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008758640.1 Rattus norvegicus conserved helix-loop-helix ubiquitous kinase (Chuk), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa46732
Clone ID Related Accession (Same CDS sequence)	XM_008760418.2 , XM_008760419.2
Accession Version	XM_008760418.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2277bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	inhibitor of nuclear factor kappa-B kinase subunit alpha isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008760418.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCGGC CCCCGGGGCT GCGGCCTGGC GCCGGCGGCC CCTGGGAGAT GCGGGAGAGG 
CTTGGCACCG GCGGCTTCGG GAACGTCAGT CTGTACCAGC ACCGGGAACT TGATCTCAAA 
ATAGCAATTA AGTCTTGTCG CTTAGAGCTA AGTACCAAAA ACAGAGAACG ATGGTGCCAT 
GAAATCCAGA TCATGAAAAA GTTGAACCAT GCAAATGTTG TAAAGGCCTG TGATGTTCCT 
GAGGAATTGA ATTTTTTAAT TAACGATGTG CCTCTTCTAG CAATGGAGTA CTGTTCTGGA 
GGGGACCTCC GGAAGCTACT CAACAAACCA GAAAATTGTT GTGGACTTAA AGAGAGCCAG 
ATCCTTTCTT TACTGAGTGA CATAGGATCT GGGATTCGTT ATTTGCATGA AAACAAAATT 
ATACATCGAG ATCTAAAACC TGAAAATATA GTGCTTCAAG ATGTTGGTGG GAAGATAATG 
CATAAAATAA TTGATTTGGG TTATGCCAAA GATGTCGATC AAGGAAGTCT CTGTACATCT 
TTTGTGGGAA CATTACAGTA TTTGGCCCCA GAGCTCTTTG AAAATAAGCC ATACACAGCC 
ACTGTGGATT ATTGGAGCTT TGGGACCATG GTGTTTGAAT GTATTGCTGG ATATAGGCCT 
TTTTTGCATC ATCTGCAGCC ATTTACCTGG CATGAAAAGA TTAAGAAGAA AGATCCAAAG 
TGTATATTTG CTTGTGAAGA GATGACCGGA GAAGTTCGGT TTAGTAGCCA TTTACCTCAG 
CCAAACAACC TTTGTAGTTT AATAGTAGAG CCAATGGAAA GCTGGCTACA GTTGATGCTG 
AATTGGGACC CACAGCAGAG AGGGGGACCT ATTGACCCTC CTTTGAAGCA GCCAAAATGC 
TTTGTGTTAA TGGATAACAT CCTCAACTTG AAGATAGTGC ACATCCTAAA TATGACTTCT 
GCAAAAATCA TTTCTTTTCT GTTACCATAT GATGAAAGTC TCCATTCACT ACAGTCTCGA 
ATTGAGCGTG AAACAGGAAT AAATACAGGT TCTCAAGAAC TTCTGTCAGA GACAGGAATT 
TCTCTGGATC CTCGGAAACC AGCCTCTCAA TGTGTTCTAG ATGGAGTGAG AGGCTGTGAT 
AGCTACATGG TTTATTTGTT TGATAAAAGT AAGACTGTAT ATGAAGGACC ATTTGCATCC 
AGAAGTTTAT CTGATTGTGT AAATTATATT GTGCAAGACA GCAAGATACA GCTTCCAGTT 
ACACAGCTTC GCAAAGTGTG GGCTGAAGCA GTGCACTATG TATCTGGGCT AAAGGAGGAC 
TACAGCAGGC TCTTTCAGGG ACAAAGAGCA GCAATGTTAA GTCTTCTTAG ATATAATGCT 
AACTTGACAA AAATGAAGAA TACTTTGATC TCAGCATCAC AGCAACTCAA AGCTAAATTG 
GAGTTTTTTC GCAAAAGCAT TCAGCTTGAC TTGGAGAGAT ATAGTGAGCA GATGACTTAT 
GGGATATCTT CAGAAAAAAT GCTGAAAGCA TGGAAAGAAA TGGAAGAAAA GGCCATTCAC 
TATTCTGAGG TTGGTGTCAT TGGATACCTT GAGGATCAAA TTATGTCTTT GCACACTGAA 
ATCATGGAGC TGCAGAAGAG CCCCTATGGG AGACGCCAGG GAGACCTGAT GGAATCCCTG 
GAACAGCGTG CCATTGATCT CTATAAGCAG CTAAAACACA GACCCACCGA TCACTCCTAC 
AGTGACAGCA CGGAGATGGT GAAGATCATT GTGCACACTG TGCAGAGTCA GGACCGAGTT 
CTCAAGGAGC TGTTTGGTCA CCTGAGCAAG TTGTTGGGCT GCAAGCAGAA GATTATTGAT 
CTACTCCCCA AGGTGGAAGT GGCCCTCAGT AACATCAAAG AAGCTGACAA TACTGTCATG 
TTTATGCAGG GAAAGAGGCA GAAAGAAATT TGGCACCTCC TTAAAATTGC TTGTACACAG 
AGTTCTGCTC GCTCTCTTGT AGGATCCAGT CTAGAAGGCA CAGTAACCCC TCCAGTATCA 
GCATGGCTGC CCCCTACTCT AGCAGACCGT GAACATCCTC TGACATGTGT GCGTAAGGTG 
TGCGTGGTCC GAGGCTCTTT GCTAGCTTGT GTACTGTCGC TTTTCTCAGA ACTCACGGAG 
ACCACCACGG CACCAAGCAC CTCCCGGAGT CTGCCCAAAA TTAGTTTTAC AGACATGCAC 
TCATGTGACA TGGCTGCTCA GGAGGGCACA CAGTGGCACA CTCTGCTGCT GTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008758640.1
CDS	97..2373
Translation	MERPPGLRPGAGGPWEMRERLGTGGFGNVSLYQHRELDLKIAIKSCRLELSTKNRERWCHEIQIMKKLNHANVVKACDVPEELNFLINDVPLLAMEYCSGGDLRKLLNKPENCCGLKESQILSLLSDIGSGIRYLHENKIIHRDLKPENIVLQDVGGKIMHKIIDLGYAKDVDQGSLCTSFVGTLQYLAPELFENKPYTATVDYWSFGTMVFECIAGYRPFLHHLQPFTWHEKIKKKDPKCIFACEEMTGEVRFSSHLPQPNNLCSLIVEPMESWLQLMLNWDPQQRGGPIDPPLKQPKCFVLMDNILNLKIVHILNMTSAKIISFLLPYDESLHSLQSRIERETGINTGSQELLSETGISLDPRKPASQCVLDGVRGCDSYMVYLFDKSKTVYEGPFASRSLSDCVNYIVQDSKIQLPVTQLRKVWAEAVHYVSGLKEDYSRLFQGQRAAMLSLLRYNANLTKMKNTLISASQQLKAKLEFFRKSIQLDLERYSEQMTYGISSEKMLKAWKEMEEKAIHYSEVGVIGYLEDQIMSLHTEIMELQKSPYGRRQGDLMESLEQRAIDLYKQLKHRPTDHSYSDSTEMVKIIVHTVQSQDRVLKELFGHLSKLLGCKQKIIDLLPKVEVALSNIKEADNTVMFMQGKRQKEIWHLLKIACTQSSARSLVGSSLEGTVTPPVSAWLPPTLADREHPLTCVRKVCVVRGSLLACVLSLFSELTETTTAPSTSRSLPKISFTDMHSCDMAAQEGTQWHTLLLC

Target ORF information:

RefSeq Version	XM_008760418.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus conserved helix-loop-helix ubiquitous kinase (Chuk), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008760418.2

ORF Insert Sequence:

ATGGAGCGGC CCCCGGGGCT GCGGCCTGGC GCCGGCGGCC CCTGGGAGAT GCGGGAGAGG 
CTTGGCACCG GCGGCTTCGG GAACGTCAGT CTGTACCAGC ACCGGGAACT TGATCTCAAA 
ATAGCAATTA AGTCTTGTCG CTTAGAGCTA AGTACCAAAA ACAGAGAACG ATGGTGCCAT 
GAAATCCAGA TCATGAAAAA GTTGAACCAT GCAAATGTTG TAAAGGCCTG TGATGTTCCT 
GAGGAATTGA ATTTTTTAAT TAACGATGTG CCTCTTCTAG CAATGGAGTA CTGTTCTGGA 
GGGGACCTCC GGAAGCTACT CAACAAACCA GAAAATTGTT GTGGACTTAA AGAGAGCCAG 
ATCCTTTCTT TACTGAGTGA CATAGGATCT GGGATTCGTT ATTTGCATGA AAACAAAATT 
ATACATCGAG ATCTAAAACC TGAAAATATA GTGCTTCAAG ATGTTGGTGG GAAGATAATG 
CATAAAATAA TTGATTTGGG TTATGCCAAA GATGTCGATC AAGGAAGTCT CTGTACATCT 
TTTGTGGGAA CATTACAGTA TTTGGCCCCA GAGCTCTTTG AAAATAAGCC ATACACAGCC 
ACTGTGGATT ATTGGAGCTT TGGGACCATG GTGTTTGAAT GTATTGCTGG ATATAGGCCT 
TTTTTGCATC ATCTGCAGCC ATTTACCTGG CATGAAAAGA TTAAGAAGAA AGATCCAAAG 
TGTATATTTG CTTGTGAAGA GATGACCGGA GAAGTTCGGT TTAGTAGCCA TTTACCTCAG 
CCAAACAACC TTTGTAGTTT AATAGTAGAG CCAATGGAAA GCTGGCTACA GTTGATGCTG 
AATTGGGACC CACAGCAGAG AGGGGGACCT ATTGACCCTC CTTTGAAGCA GCCAAAATGC 
TTTGTGTTAA TGGATAACAT CCTCAACTTG AAGATAGTGC ACATCCTAAA TATGACTTCT 
GCAAAAATCA TTTCTTTTCT GTTACCATAT GATGAAAGTC TCCATTCACT ACAGTCTCGA 
ATTGAGCGTG AAACAGGAAT AAATACAGGT TCTCAAGAAC TTCTGTCAGA GACAGGAATT 
TCTCTGGATC CTCGGAAACC AGCCTCTCAA TGTGTTCTAG ATGGAGTGAG AGGCTGTGAT 
AGCTACATGG TTTATTTGTT TGATAAAAGT AAGACTGTAT ATGAAGGACC ATTTGCATCC 
AGAAGTTTAT CTGATTGTGT AAATTATATT GTGCAAGACA GCAAGATACA GCTTCCAGTT 
ACACAGCTTC GCAAAGTGTG GGCTGAAGCA GTGCACTATG TATCTGGGCT AAAGGAGGAC 
TACAGCAGGC TCTTTCAGGG ACAAAGAGCA GCAATGTTAA GTCTTCTTAG ATATAATGCT 
AACTTGACAA AAATGAAGAA TACTTTGATC TCAGCATCAC AGCAACTCAA AGCTAAATTG 
GAGTTTTTTC GCAAAAGCAT TCAGCTTGAC TTGGAGAGAT ATAGTGAGCA GATGACTTAT 
GGGATATCTT CAGAAAAAAT GCTGAAAGCA TGGAAAGAAA TGGAAGAAAA GGCCATTCAC 
TATTCTGAGG TTGGTGTCAT TGGATACCTT GAGGATCAAA TTATGTCTTT GCACACTGAA 
ATCATGGAGC TGCAGAAGAG CCCCTATGGG AGACGCCAGG GAGACCTGAT GGAATCCCTG 
GAACAGCGTG CCATTGATCT CTATAAGCAG CTAAAACACA GACCCACCGA TCACTCCTAC 
AGTGACAGCA CGGAGATGGT GAAGATCATT GTGCACACTG TGCAGAGTCA GGACCGAGTT 
CTCAAGGAGC TGTTTGGTCA CCTGAGCAAG TTGTTGGGCT GCAAGCAGAA GATTATTGAT 
CTACTCCCCA AGGTGGAAGT GGCCCTCAGT AACATCAAAG AAGCTGACAA TACTGTCATG 
TTTATGCAGG GAAAGAGGCA GAAAGAAATT TGGCACCTCC TTAAAATTGC TTGTACACAG 
AGTTCTGCTC GCTCTCTTGT AGGATCCAGT CTAGAAGGCA CAGTAACCCC TCCAGTATCA 
GCATGGCTGC CCCCTACTCT AGCAGACCGT GAACATCCTC TGACATGTGT GCGTAAGGTG 
TGCGTGGTCC GAGGCTCTTT GCTAGCTTGT GTACTGTCGC TTTTCTCAGA ACTCACGGAG 
ACCACCACGG CACCAAGCAC CTCCCGGAGT CTGCCCAAAA TTAGTTTTAC AGACATGCAC 
TCATGTGACA TGGCTGCTCA GGAGGGCACA CAGTGGCACA CTCTGCTGCT GTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Chuk ( XM_008760419.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008758641.1 Rattus norvegicus conserved helix-loop-helix ubiquitous kinase (Chuk), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa46732
Clone ID Related Accession (Same CDS sequence)	XM_008760418.2 , XM_008760419.2
Accession Version	XM_008760419.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2277bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	inhibitor of nuclear factor kappa-B kinase subunit alpha isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_008760419.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCGGC CCCCGGGGCT GCGGCCTGGC GCCGGCGGCC CCTGGGAGAT GCGGGAGAGG 
CTTGGCACCG GCGGCTTCGG GAACGTCAGT CTGTACCAGC ACCGGGAACT TGATCTCAAA 
ATAGCAATTA AGTCTTGTCG CTTAGAGCTA AGTACCAAAA ACAGAGAACG ATGGTGCCAT 
GAAATCCAGA TCATGAAAAA GTTGAACCAT GCAAATGTTG TAAAGGCCTG TGATGTTCCT 
GAGGAATTGA ATTTTTTAAT TAACGATGTG CCTCTTCTAG CAATGGAGTA CTGTTCTGGA 
GGGGACCTCC GGAAGCTACT CAACAAACCA GAAAATTGTT GTGGACTTAA AGAGAGCCAG 
ATCCTTTCTT TACTGAGTGA CATAGGATCT GGGATTCGTT ATTTGCATGA AAACAAAATT 
ATACATCGAG ATCTAAAACC TGAAAATATA GTGCTTCAAG ATGTTGGTGG GAAGATAATG 
CATAAAATAA TTGATTTGGG TTATGCCAAA GATGTCGATC AAGGAAGTCT CTGTACATCT 
TTTGTGGGAA CATTACAGTA TTTGGCCCCA GAGCTCTTTG AAAATAAGCC ATACACAGCC 
ACTGTGGATT ATTGGAGCTT TGGGACCATG GTGTTTGAAT GTATTGCTGG ATATAGGCCT 
TTTTTGCATC ATCTGCAGCC ATTTACCTGG CATGAAAAGA TTAAGAAGAA AGATCCAAAG 
TGTATATTTG CTTGTGAAGA GATGACCGGA GAAGTTCGGT TTAGTAGCCA TTTACCTCAG 
CCAAACAACC TTTGTAGTTT AATAGTAGAG CCAATGGAAA GCTGGCTACA GTTGATGCTG 
AATTGGGACC CACAGCAGAG AGGGGGACCT ATTGACCCTC CTTTGAAGCA GCCAAAATGC 
TTTGTGTTAA TGGATAACAT CCTCAACTTG AAGATAGTGC ACATCCTAAA TATGACTTCT 
GCAAAAATCA TTTCTTTTCT GTTACCATAT GATGAAAGTC TCCATTCACT ACAGTCTCGA 
ATTGAGCGTG AAACAGGAAT AAATACAGGT TCTCAAGAAC TTCTGTCAGA GACAGGAATT 
TCTCTGGATC CTCGGAAACC AGCCTCTCAA TGTGTTCTAG ATGGAGTGAG AGGCTGTGAT 
AGCTACATGG TTTATTTGTT TGATAAAAGT AAGACTGTAT ATGAAGGACC ATTTGCATCC 
AGAAGTTTAT CTGATTGTGT AAATTATATT GTGCAAGACA GCAAGATACA GCTTCCAGTT 
ACACAGCTTC GCAAAGTGTG GGCTGAAGCA GTGCACTATG TATCTGGGCT AAAGGAGGAC 
TACAGCAGGC TCTTTCAGGG ACAAAGAGCA GCAATGTTAA GTCTTCTTAG ATATAATGCT 
AACTTGACAA AAATGAAGAA TACTTTGATC TCAGCATCAC AGCAACTCAA AGCTAAATTG 
GAGTTTTTTC GCAAAAGCAT TCAGCTTGAC TTGGAGAGAT ATAGTGAGCA GATGACTTAT 
GGGATATCTT CAGAAAAAAT GCTGAAAGCA TGGAAAGAAA TGGAAGAAAA GGCCATTCAC 
TATTCTGAGG TTGGTGTCAT TGGATACCTT GAGGATCAAA TTATGTCTTT GCACACTGAA 
ATCATGGAGC TGCAGAAGAG CCCCTATGGG AGACGCCAGG GAGACCTGAT GGAATCCCTG 
GAACAGCGTG CCATTGATCT CTATAAGCAG CTAAAACACA GACCCACCGA TCACTCCTAC 
AGTGACAGCA CGGAGATGGT GAAGATCATT GTGCACACTG TGCAGAGTCA GGACCGAGTT 
CTCAAGGAGC TGTTTGGTCA CCTGAGCAAG TTGTTGGGCT GCAAGCAGAA GATTATTGAT 
CTACTCCCCA AGGTGGAAGT GGCCCTCAGT AACATCAAAG AAGCTGACAA TACTGTCATG 
TTTATGCAGG GAAAGAGGCA GAAAGAAATT TGGCACCTCC TTAAAATTGC TTGTACACAG 
AGTTCTGCTC GCTCTCTTGT AGGATCCAGT CTAGAAGGCA CAGTAACCCC TCCAGTATCA 
GCATGGCTGC CCCCTACTCT AGCAGACCGT GAACATCCTC TGACATGTGT GCGTAAGGTG 
TGCGTGGTCC GAGGCTCTTT GCTAGCTTGT GTACTGTCGC TTTTCTCAGA ACTCACGGAG 
ACCACCACGG CACCAAGCAC CTCCCGGAGT CTGCCCAAAA TTAGTTTTAC AGACATGCAC 
TCATGTGACA TGGCTGCTCA GGAGGGCACA CAGTGGCACA CTCTGCTGCT GTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008758641.1
CDS	97..2373
Translation	MERPPGLRPGAGGPWEMRERLGTGGFGNVSLYQHRELDLKIAIKSCRLELSTKNRERWCHEIQIMKKLNHANVVKACDVPEELNFLINDVPLLAMEYCSGGDLRKLLNKPENCCGLKESQILSLLSDIGSGIRYLHENKIIHRDLKPENIVLQDVGGKIMHKIIDLGYAKDVDQGSLCTSFVGTLQYLAPELFENKPYTATVDYWSFGTMVFECIAGYRPFLHHLQPFTWHEKIKKKDPKCIFACEEMTGEVRFSSHLPQPNNLCSLIVEPMESWLQLMLNWDPQQRGGPIDPPLKQPKCFVLMDNILNLKIVHILNMTSAKIISFLLPYDESLHSLQSRIERETGINTGSQELLSETGISLDPRKPASQCVLDGVRGCDSYMVYLFDKSKTVYEGPFASRSLSDCVNYIVQDSKIQLPVTQLRKVWAEAVHYVSGLKEDYSRLFQGQRAAMLSLLRYNANLTKMKNTLISASQQLKAKLEFFRKSIQLDLERYSEQMTYGISSEKMLKAWKEMEEKAIHYSEVGVIGYLEDQIMSLHTEIMELQKSPYGRRQGDLMESLEQRAIDLYKQLKHRPTDHSYSDSTEMVKIIVHTVQSQDRVLKELFGHLSKLLGCKQKIIDLLPKVEVALSNIKEADNTVMFMQGKRQKEIWHLLKIACTQSSARSLVGSSLEGTVTPPVSAWLPPTLADREHPLTCVRKVCVVRGSLLACVLSLFSELTETTTAPSTSRSLPKISFTDMHSCDMAAQEGTQWHTLLLC

Target ORF information:

RefSeq Version	XM_008760419.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus conserved helix-loop-helix ubiquitous kinase (Chuk), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008760419.2

ORF Insert Sequence:

ATGGAGCGGC CCCCGGGGCT GCGGCCTGGC GCCGGCGGCC CCTGGGAGAT GCGGGAGAGG 
CTTGGCACCG GCGGCTTCGG GAACGTCAGT CTGTACCAGC ACCGGGAACT TGATCTCAAA 
ATAGCAATTA AGTCTTGTCG CTTAGAGCTA AGTACCAAAA ACAGAGAACG ATGGTGCCAT 
GAAATCCAGA TCATGAAAAA GTTGAACCAT GCAAATGTTG TAAAGGCCTG TGATGTTCCT 
GAGGAATTGA ATTTTTTAAT TAACGATGTG CCTCTTCTAG CAATGGAGTA CTGTTCTGGA 
GGGGACCTCC GGAAGCTACT CAACAAACCA GAAAATTGTT GTGGACTTAA AGAGAGCCAG 
ATCCTTTCTT TACTGAGTGA CATAGGATCT GGGATTCGTT ATTTGCATGA AAACAAAATT 
ATACATCGAG ATCTAAAACC TGAAAATATA GTGCTTCAAG ATGTTGGTGG GAAGATAATG 
CATAAAATAA TTGATTTGGG TTATGCCAAA GATGTCGATC AAGGAAGTCT CTGTACATCT 
TTTGTGGGAA CATTACAGTA TTTGGCCCCA GAGCTCTTTG AAAATAAGCC ATACACAGCC 
ACTGTGGATT ATTGGAGCTT TGGGACCATG GTGTTTGAAT GTATTGCTGG ATATAGGCCT 
TTTTTGCATC ATCTGCAGCC ATTTACCTGG CATGAAAAGA TTAAGAAGAA AGATCCAAAG 
TGTATATTTG CTTGTGAAGA GATGACCGGA GAAGTTCGGT TTAGTAGCCA TTTACCTCAG 
CCAAACAACC TTTGTAGTTT AATAGTAGAG CCAATGGAAA GCTGGCTACA GTTGATGCTG 
AATTGGGACC CACAGCAGAG AGGGGGACCT ATTGACCCTC CTTTGAAGCA GCCAAAATGC 
TTTGTGTTAA TGGATAACAT CCTCAACTTG AAGATAGTGC ACATCCTAAA TATGACTTCT 
GCAAAAATCA TTTCTTTTCT GTTACCATAT GATGAAAGTC TCCATTCACT ACAGTCTCGA 
ATTGAGCGTG AAACAGGAAT AAATACAGGT TCTCAAGAAC TTCTGTCAGA GACAGGAATT 
TCTCTGGATC CTCGGAAACC AGCCTCTCAA TGTGTTCTAG ATGGAGTGAG AGGCTGTGAT 
AGCTACATGG TTTATTTGTT TGATAAAAGT AAGACTGTAT ATGAAGGACC ATTTGCATCC 
AGAAGTTTAT CTGATTGTGT AAATTATATT GTGCAAGACA GCAAGATACA GCTTCCAGTT 
ACACAGCTTC GCAAAGTGTG GGCTGAAGCA GTGCACTATG TATCTGGGCT AAAGGAGGAC 
TACAGCAGGC TCTTTCAGGG ACAAAGAGCA GCAATGTTAA GTCTTCTTAG ATATAATGCT 
AACTTGACAA AAATGAAGAA TACTTTGATC TCAGCATCAC AGCAACTCAA AGCTAAATTG 
GAGTTTTTTC GCAAAAGCAT TCAGCTTGAC TTGGAGAGAT ATAGTGAGCA GATGACTTAT 
GGGATATCTT CAGAAAAAAT GCTGAAAGCA TGGAAAGAAA TGGAAGAAAA GGCCATTCAC 
TATTCTGAGG TTGGTGTCAT TGGATACCTT GAGGATCAAA TTATGTCTTT GCACACTGAA 
ATCATGGAGC TGCAGAAGAG CCCCTATGGG AGACGCCAGG GAGACCTGAT GGAATCCCTG 
GAACAGCGTG CCATTGATCT CTATAAGCAG CTAAAACACA GACCCACCGA TCACTCCTAC 
AGTGACAGCA CGGAGATGGT GAAGATCATT GTGCACACTG TGCAGAGTCA GGACCGAGTT 
CTCAAGGAGC TGTTTGGTCA CCTGAGCAAG TTGTTGGGCT GCAAGCAGAA GATTATTGAT 
CTACTCCCCA AGGTGGAAGT GGCCCTCAGT AACATCAAAG AAGCTGACAA TACTGTCATG 
TTTATGCAGG GAAAGAGGCA GAAAGAAATT TGGCACCTCC TTAAAATTGC TTGTACACAG 
AGTTCTGCTC GCTCTCTTGT AGGATCCAGT CTAGAAGGCA CAGTAACCCC TCCAGTATCA 
GCATGGCTGC CCCCTACTCT AGCAGACCGT GAACATCCTC TGACATGTGT GCGTAAGGTG 
TGCGTGGTCC GAGGCTCTTT GCTAGCTTGT GTACTGTCGC TTTTCTCAGA ACTCACGGAG 
ACCACCACGG CACCAAGCAC CTCCCGGAGT CTGCCCAAAA TTAGTTTTAC AGACATGCAC 
TCATGTGACA TGGCTGCTCA GGAGGGCACA CAGTGGCACA CTCTGCTGCT GTGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Chuk ( XM_017589269.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017444758.1 Rattus norvegicus conserved helix-loop-helix ubiquitous kinase (Chuk), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ORa56642
Clone ID Related Accession (Same CDS sequence)	XM_017589269.1
Accession Version	XM_017589269.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2142bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	inhibitor of nuclear factor kappa-B kinase subunit alpha isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCGGC CCCCGGGGCT GCGGCCTGGC GCCGGCGGCC CCTGGGAGAT GCGGGAGAGG 
CTTGGCACCG GCGGCTTCGG GAACGTCAGT CTGTACCAGC ACCGGGAACT TGATCTCAAA 
ATAGCAATTA AGTCTTGTCG CTTAGAGCTA AGTACCAAAA ACAGAGAACG ATGGTGCCAT 
GAAATCCAGA TCATGAAAAA GTTGAACCAT GCAAATGTTG TAAAGGCCTG TGATGTTCCT 
GAGGAATTGA ATTTTTTAAT TAACGATGTG CCTCTTCTAG CAATGGAGTA CTGTTCTGGA 
GGGGACCTCC GGAAGCTACT CAACAAACCA GAAAATTGTT GTGGACTTAA AGAGAGCCAG 
ATCCTTTCTT TACTGAGTGA CATAGGATCT GGGATTCGTT ATTTGCATGA AAACAAAATT 
ATACATCGAG ATCTAAAACC TGAAAATATA GTGCTTCAAG ATGTTGGTGG GAAGATAATG 
CATAAAATAA TTGATTTGGG TTATGCCAAA GATGTCGATC AAGGAAGTCT CTGTACATCT 
TTTGTGGGAA CATTACAGTA TTTGGCCCCA GAGCTCTTTG AAAATAAGCC ATACACAGCC 
ACTGTGGATT ATTGGAGCTT TGGGACCATG GTGTTTGAAT GTATTGCTGG ATATAGGCCT 
TTTTTGCATC ATCTGCAGCC ATTTACCTGG CATGAAAAGA TTAAGAAGAA AGATCCAAAG 
TGTATATTTG CTTGTGAAGA GATGACCGGA GAAGTTCGGT TTAGTAGCCA TTTACCTCAG 
CCAAACAACC TTTGTAGTTT AATAGTAGAG CCAATGGAAA GCTGGCTACA GTTGATGCTG 
AATTGGGACC CACAGCAGAG AGGGGGACCT ATTGACCCTC CTTTGAAGCA GCCAAAATGC 
TTTGTGTTAA TGGATAACAT CCTCAACTTG AAGATAGTGC ACATCCTAAA TATGACTTCT 
GCAAAAATCA TTTCTTTTCT GTTACCATAT GATGAAAGTC TCCATTCACT ACAGTCTCGA 
ATTGAGCGTG AAACAGGAAT AAATACAGGT TCTCAAGAAC TTCTGTCAGA GACAGGAATT 
TCTCTGGATC CTCGGAAACC AGCCTCTCAA TGTGTTCTAG ATGGAGTGAG AGGCTGTGAT 
AGCTACATGG TTTATTTGTT TGATAAAAGT AAGACTGTAT ATGAAGGACC ATTTGCATCC 
AGAAGTTTAT CTGATTGTGT AAATTATATT GTGCAAGACA GCAAGATACA GCTTCCAGTT 
ACACAGCTTC GCAAAGTGTG GGCTGAAGCA GTGCACTATG TATCTGGGCT AAAGGAGGAC 
TACAGCAGGC TCTTTCAGGG ACAAAGAGCA GCAATGTTAA GTCTTCTTAG ATATAATGCT 
AACTTGACAA AAATGAAGAA TACTTTGATC TCAGCATCAC AGCAACTCAA AGCTAAATTG 
GAGTTTTTTC GCAAAAGCAT TCAGCTTGAC TTGGAGAGAT ATAGTGAGCA GATGACTTAT 
GGGATATCTT CAGAAAAAAT GCTGAAAGCA TGGAAAGAAA TGGAAGAAAA GGCCATTCAC 
TATTCTGAGG TTGGTGTCAT TGGATACCTT GAGGATCAAA TTATGTCTTT GCACACTGAA 
ATCATGGAGC TGCAGAAGAG CCCCTATGGG AGACGCCAGG GAGACCTGAT GGAATCCCTG 
GAACAGCGTG CCATTGATCT CTATAAGCAG CTAAAACACA GACCCACCGA TCACTCCTAC 
AGTGACAGCA CGGAGATGGT GAAGATCATT GTGCACACTG TGCAGAGTCA GGACCGAGTT 
CTCAAGGAGC TGTTTGGTCA CCTGAGCAAG TTGTTGGGCT GCAAGCAGAA GATTATTGAT 
CTACTCCCCA AGGTGGAAGT GGCCCTCAGT AACATCAAAG AAGCTGACAA TACTGTCATG 
TTTATGCAGG GAAAGAGGCA GAAAGAAATT TGGCACCTCC TTAAAATTGC TTGTACACAG 
AGTTCTGCTC GCTCTCTTGT AGGATCCAGT CTAGAAGGCA CAGTAACCCC TCCAGTATCA 
GCATGGCTGC CCCCTACTCT AGCAGACCGT GAACATCCTC TGACATGTGT GGTAACTCCT 
CAAGATGGCG TAAGGTGTGC GTGGTCCGAG GCTCTTTGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017444758.1
CDS	96..2237
Translation	MERPPGLRPGAGGPWEMRERLGTGGFGNVSLYQHRELDLKIAIKSCRLELSTKNRERWCHEIQIMKKLNHANVVKACDVPEELNFLINDVPLLAMEYCSGGDLRKLLNKPENCCGLKESQILSLLSDIGSGIRYLHENKIIHRDLKPENIVLQDVGGKIMHKIIDLGYAKDVDQGSLCTSFVGTLQYLAPELFENKPYTATVDYWSFGTMVFECIAGYRPFLHHLQPFTWHEKIKKKDPKCIFACEEMTGEVRFSSHLPQPNNLCSLIVEPMESWLQLMLNWDPQQRGGPIDPPLKQPKCFVLMDNILNLKIVHILNMTSAKIISFLLPYDESLHSLQSRIERETGINTGSQELLSETGISLDPRKPASQCVLDGVRGCDSYMVYLFDKSKTVYEGPFASRSLSDCVNYIVQDSKIQLPVTQLRKVWAEAVHYVSGLKEDYSRLFQGQRAAMLSLLRYNANLTKMKNTLISASQQLKAKLEFFRKSIQLDLERYSEQMTYGISSEKMLKAWKEMEEKAIHYSEVGVIGYLEDQIMSLHTEIMELQKSPYGRRQGDLMESLEQRAIDLYKQLKHRPTDHSYSDSTEMVKIIVHTVQSQDRVLKELFGHLSKLLGCKQKIIDLLPKVEVALSNIKEADNTVMFMQGKRQKEIWHLLKIACTQSSARSLVGSSLEGTVTPPVSAWLPPTLADREHPLTCVVTPQDGVRCAWSEALC

Target ORF information:

RefSeq Version	XM_017589269.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus conserved helix-loop-helix ubiquitous kinase (Chuk), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017589269.1

ORF Insert Sequence:

ATGGAGCGGC CCCCGGGGCT GCGGCCTGGC GCCGGCGGCC CCTGGGAGAT GCGGGAGAGG 
CTTGGCACCG GCGGCTTCGG GAACGTCAGT CTGTACCAGC ACCGGGAACT TGATCTCAAA 
ATAGCAATTA AGTCTTGTCG CTTAGAGCTA AGTACCAAAA ACAGAGAACG ATGGTGCCAT 
GAAATCCAGA TCATGAAAAA GTTGAACCAT GCAAATGTTG TAAAGGCCTG TGATGTTCCT 
GAGGAATTGA ATTTTTTAAT TAACGATGTG CCTCTTCTAG CAATGGAGTA CTGTTCTGGA 
GGGGACCTCC GGAAGCTACT CAACAAACCA GAAAATTGTT GTGGACTTAA AGAGAGCCAG 
ATCCTTTCTT TACTGAGTGA CATAGGATCT GGGATTCGTT ATTTGCATGA AAACAAAATT 
ATACATCGAG ATCTAAAACC TGAAAATATA GTGCTTCAAG ATGTTGGTGG GAAGATAATG 
CATAAAATAA TTGATTTGGG TTATGCCAAA GATGTCGATC AAGGAAGTCT CTGTACATCT 
TTTGTGGGAA CATTACAGTA TTTGGCCCCA GAGCTCTTTG AAAATAAGCC ATACACAGCC 
ACTGTGGATT ATTGGAGCTT TGGGACCATG GTGTTTGAAT GTATTGCTGG ATATAGGCCT 
TTTTTGCATC ATCTGCAGCC ATTTACCTGG CATGAAAAGA TTAAGAAGAA AGATCCAAAG 
TGTATATTTG CTTGTGAAGA GATGACCGGA GAAGTTCGGT TTAGTAGCCA TTTACCTCAG 
CCAAACAACC TTTGTAGTTT AATAGTAGAG CCAATGGAAA GCTGGCTACA GTTGATGCTG 
AATTGGGACC CACAGCAGAG AGGGGGACCT ATTGACCCTC CTTTGAAGCA GCCAAAATGC 
TTTGTGTTAA TGGATAACAT CCTCAACTTG AAGATAGTGC ACATCCTAAA TATGACTTCT 
GCAAAAATCA TTTCTTTTCT GTTACCATAT GATGAAAGTC TCCATTCACT ACAGTCTCGA 
ATTGAGCGTG AAACAGGAAT AAATACAGGT TCTCAAGAAC TTCTGTCAGA GACAGGAATT 
TCTCTGGATC CTCGGAAACC AGCCTCTCAA TGTGTTCTAG ATGGAGTGAG AGGCTGTGAT 
AGCTACATGG TTTATTTGTT TGATAAAAGT AAGACTGTAT ATGAAGGACC ATTTGCATCC 
AGAAGTTTAT CTGATTGTGT AAATTATATT GTGCAAGACA GCAAGATACA GCTTCCAGTT 
ACACAGCTTC GCAAAGTGTG GGCTGAAGCA GTGCACTATG TATCTGGGCT AAAGGAGGAC 
TACAGCAGGC TCTTTCAGGG ACAAAGAGCA GCAATGTTAA GTCTTCTTAG ATATAATGCT 
AACTTGACAA AAATGAAGAA TACTTTGATC TCAGCATCAC AGCAACTCAA AGCTAAATTG 
GAGTTTTTTC GCAAAAGCAT TCAGCTTGAC TTGGAGAGAT ATAGTGAGCA GATGACTTAT 
GGGATATCTT CAGAAAAAAT GCTGAAAGCA TGGAAAGAAA TGGAAGAAAA GGCCATTCAC 
TATTCTGAGG TTGGTGTCAT TGGATACCTT GAGGATCAAA TTATGTCTTT GCACACTGAA 
ATCATGGAGC TGCAGAAGAG CCCCTATGGG AGACGCCAGG GAGACCTGAT GGAATCCCTG 
GAACAGCGTG CCATTGATCT CTATAAGCAG CTAAAACACA GACCCACCGA TCACTCCTAC 
AGTGACAGCA CGGAGATGGT GAAGATCATT GTGCACACTG TGCAGAGTCA GGACCGAGTT 
CTCAAGGAGC TGTTTGGTCA CCTGAGCAAG TTGTTGGGCT GCAAGCAGAA GATTATTGAT 
CTACTCCCCA AGGTGGAAGT GGCCCTCAGT AACATCAAAG AAGCTGACAA TACTGTCATG 
TTTATGCAGG GAAAGAGGCA GAAAGAAATT TGGCACCTCC TTAAAATTGC TTGTACACAG 
AGTTCTGCTC GCTCTCTTGT AGGATCCAGT CTAGAAGGCA CAGTAACCCC TCCAGTATCA 
GCATGGCTGC CCCCTACTCT AGCAGACCGT GAACATCCTC TGACATGTGT GGTAACTCCT 
CAAGATGGCG TAAGGTGTGC GTGGTCCGAG GCTCTTTGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Cadmium induces matrix metalloproteinase-9 expression via ROS-dependent EGFR, NF-?B, and AP-1 pathways in human endothelial cells.
Toxicology338104-16(2015 Dec)
Lian S,Xia Y,Khoi PN,Ung TT,Yoon HJ,Kim NH,Kim KK,Jung YD

Apigenin blocks IKK? activation and suppresses prostate cancer progression.
Oncotarget6(31)31216-32(2015 Oct)
Shukla S,Kanwal R,Shankar E,Datt M,Chance MR,Fu P,MacLennan GT,Gupta S

Suppression of NF-?B and NF-?B-Regulated Gene Expression by Apigenin through I?B? and IKK Pathway in TRAMP Mice.
PloS one10(9)e0138710(2015)
Shukla S,Shankar E,Fu P,MacLennan GT,Gupta S

IKK regulates the deubiquitinase CYLD at the postsynaptic density.
Biochemical and biophysical research communications450(1)550-4(2014 Jul)
Thein S,Pham A,Bayer KU,Tao-Cheng JH,Dosemeci A

A new transcriptional role for matrix metalloproteinase-12 in antiviral immunity.
Nature medicine20(5)493-502(2014 May)
Marchant DJ,Bellac CL,Moraes TJ,Wadsworth SJ,Dufour A,Butler GS,Bilawchuk LM,Hendry RG,Robertson AG,Cheung CT,Ng J,Ang L,Luo Z,Heilbron K,Norris MJ,Duan W,Bucyk T,Karpov A,Devel L,Georgiadis D,Hegele RG,Luo H,Granville DJ,Dive V,McManus BM,Overall CM

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Chuk cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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