Tpcn2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Tpcn2
Entrez Gene ID	309139
Full Name	two pore segment channel 2
General protein information	Preferred Names two pore segment channel 2 Names two pore calcium channel protein 2
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 1 Map Location: 1q42

mRNA and Protein(s)

mRNA	Protein	Name
NM_001107566.1	NP_001101036.1	two pore calcium channel protein 2
XM_006230784.3	XP_006230846.1	two pore calcium channel protein 2 isoform X1

Pathways from BioSystems

Homologies

	TPCN2	XP_001101571.1
Bos taurus (cattle)	TPCN2	NP_001179380.1
Danio rerio (zebrafish)	tpcn2	NP_001071190.1
Xenopus tropicalis (tropical clawed frog)	tpcn2	XP_002934116.2
Pan troglodytes (chimpanzee)	TPCN2	XP_001173935.2
Canis lupus familiaris (dog)	TPCN2	XP_540804.3
Mus musculus (house mouse)	Tpcn2	NP_666318.2
Rattus norvegicus (Norway rat)	Tpcn2	NP_001101036.1
Gallus gallus (chicken)	TPCN2	XP_004941585.1
Homo sapiens (human)	TPCN2	NP_620714.2

Gene Ontology
Bibliography

Related articles in PubMed

Dysregulation of lysosomal morphology by pathogenic LRRK2 is corrected by TPC2 inhibition.
Hockey LN, Kilpatrick BS, Eden ER, Lin-Moshier Y, Brailoiu GC, Brailoiu E, Futter CE, Schapira AH, Marchant JS, Patel S
Journal of cell science128(2)232-8(2015 Jan)

Identification of a novel gene for diabetic traits in rats, mice, and humans.
Tsaih SW, Holl K, Jia S, Kaldunski M, Tschannen M, He H, Andrae JW, Li SH, Stoddard A, Wiederhold A, Parrington J, Ruas da Silva M, Galione A, Meigs J, , Hoffmann RG, Simpson P, Jacob H, Hessner M, Solberg Woods LC
Genetics198(1)17-29(2014 Sep)

Leucine-rich repeat kinase 2 regulates autophagy through a calcium-dependent pathway involving NAADP.
G?mez-Suaga P, Luz?n-Toro B, Churamani D, Zhang L, Bloor-Young D, Patel S, Woodman PG, Churchill GC, Hilfiker S
Human molecular genetics21(3)511-25(2012 Feb)

Two-pore channels form homo- and heterodimers.
Rietdorf K, Funnell TM, Ruas M, Heinemann J, Parrington J, Galione A
The Journal of biological chemistry286(43)37058-62(2011 Oct)

TPC2 proteins mediate nicotinic acid adenine dinucleotide phosphate (NAADP)- and agonist-evoked contractions of smooth muscle.
Tugba Durlu-Kandilci N, Ruas M, Chuang KT, Brading A, Parrington J, Galione A
The Journal of biological chemistry285(32)24925-32(2010 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Tpcn2 was identified as a novel gene for diabetic traits in rats.
Title: Identification of a novel gene for diabetic traits in rats, mice, and humans.

The following Tpcn2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Tpcn2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa05437	NM_001107566.1 Latest version!	Rattus norvegicus two pore segment channel 2 (Tpcn2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$419.30 $599.00
ORa23761	XM_006230784.3 Latest version!	Rattus norvegicus two pore segment channel 2 (Tpcn2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Tpcn2 ( NM_001107566.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001101036.1 Rattus norvegicus two pore segment channel 2 (Tpcn2), mRNA.

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CloneID	ORa05437
Clone ID Related Accession (Same CDS sequence)	NM_001107566.1
Accession Version	NM_001107566.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2136bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-02
Organism	Rattus norvegicus(Norway rat)
Product	two pore calcium channel protein 2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CH473953.1. On or before Oct 4, 2007 this sequence version replaced XM_219555.4, XM_001067197.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGGCGGCAG AAGCGCAGCC CCTTCTGGGC CGGGACCGAG GTGGTGGCCA GGCCCACTCT 
GGCGCTGATC AGGAGCTCTG CATAGACCAG GCTGTGGTCT TCATTGAAGA TGCCATAAAG 
TACCGATCCA TCTACCACCG CCTGGACGCT GGCTCACTGT GGCTTTACCG CTGGTATTAC 
TCGAACGTGT GCCAACGAGT GCTGGGCTTC ACCATTTTCC TGATCCTGGC CTTGGCTTTT 
GTGGAGGTCC CATCTTCCTT CACAAGGACT GCGGATGTGC GCTACCGCTC CCAGCCCTGG 
CAGCCACCCT GTGGCCTGAC AGAGACAATT GAGGCGCTCT GCTTGCTGGT CTTCCTGGCT 
GACCTCTCTG TGAAGGGCTA CCTGGTGGGG CAGGCCCAGT TGCAGCAGAA CCTGTGGCTG 
CTGGCTTACT TTGTGGTGCT GGTTGTGTCT GTGGTGGACT GGATCGTTTC GCTGAGCCTC 
AACTGTGAGG AGTCTCTGCG GGTGCGCCGG CTACTCCGAC CCTTCTTCCT GCTGCAGAAT 
TCCTCCATGA TGAAGAAGAC CCTGAAATGT ATACGGTGGT CACTACCAGA AATGGCTAGT 
GTTGGGCTGC TGCTGACTAT CCACCTGTGT CTCTTCACCG TAATCGGGAT GCTGCTGTTC 
ACCATTGCTG AGAAGGATGA AGCACAGAAC AAGGAGAGGC TGACCTACTT CCGGAACCTT 
CCAGAAGCAC TGACCTCACT CCTAGTGCTG CTGACTACTT CCAACAACCC TGATGGAAGC 
TTGTTTCTGA TGAACCTGCT GACGGCCATC ATCTATAATC AGTTCCGAGG CTACCTAATG 
AAATCTCTAC AGACTTCACT GTTCCGGCGG CGACTAGGGG CCCGTGCGGC CTATGAAGTC 
CTCACCCTTC AGGCAGGGCC AAATGGAACC ACCTCTGAGT TAGTCGGGGT AAATCCCGAG 
AACTTCCTGC GAGTGCTTCA GAAAACCCAG CTGAACAAAA TCCACAAACA AGCCATCATA 
CAGAAGGTGC ACTCCTATGA AGGCCGCCCG ATCCTGGCCG ATGAGTTCCA GAAACTCTTT 
GATGAGGTTG ACAAAGGTAT AGTCAAAGAG CGCCCGCCGA AGCCCCAGTA CCAGTCTCCG 
TTTCTGCAGA CTGCCCAGTT CATCTTCAGC CACCGCTACT TTGACTACCT GGGGAACCTC 
ATTGCTCTGG GAAACCTCTT GTCTATTTGT GTGTTCCTGG TGCTGGACTC AAACCTGCTG 
CCTGGGGAAC GGAACGACTT TGTCCTGGGG ATTCTCAACT ACATTTTCAT CTTGTACTAC 
CTGCTGGAGC TGCTGTTCAA GGTGTTCGCG CTGGGCCTAC GGGGCTACCT GTTCTACCAC 
AGCAACGTGT TCGATGGCCT CCTGACCATC ATCCTGCTGG TTTTGGAGAT TTCTACTCTG 
GCCGTGTACC GATTGCCGCA CCCAGGATGG AAGCCAGAGC AGTATGGCCC CCTGTCGCTT 
TGGGACATGA CTCGGCTGGT GAACATTCTG ATTGTGATAC GCTTCCTGCG CATCATCCCC 
AGCATAAAGC CAACGGCTGT GGTAGCCAGC ACCATCCTGG GCCTAATCCA GAACCTGCGG 
GCATTTGGTG GGATCCTGGT GGTGGTATAC TATGTGTTCG CCATGATTGG GATCAACCTG 
TTCCGAGGTG TCATTGTGCC TCCCGGAAAC AGCAGCCTGG TTCCTGATAA CAGCTCAGCT 
CCGTGTGGAA GCTTTGAGCA GCTAGGTTAC TGGCCCAACA ACTTCGACGA CTTTGCTGCT 
GCTCTGATCA CACTGTGGGA CGTGATGGTG GTGAACAATT GGCAGGTGAT CCTGGAAGCC 
TATAAGCACT ACTCCGGCCC GTGGTCGATG GTGTATTTTG TGCTGTGGTG GTTGGTGTCC 
TCTGTCATCT GGATCAACCT GTTTCTGGCT CTGCTTCTGG AGAACTTTCT CCACAGATGG 
GACCCTCAAG GTCATAAGCA GCTCCTGGTT GGGACCAAGC AGATCACCTA CGAGATGTCT 
GTGGAGCTCA TGTTCAGAGA TATCCTAGAA GAGCCCAAGG AGGAGGAACT GATGGAGAAG 
CTACACAAGC ATCCACACTT GCATCTGTGC AGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001101036.1
CDS	81..2216
Translation	MAAEAQPLLGRDRGGGQAHSGADQELCIDQAVVFIEDAIKYRSIYHRLDAGSLWLYRWYYSNVCQRVLGFTIFLILALAFVEVPSSFTRTADVRYRSQPWQPPCGLTETIEALCLLVFLADLSVKGYLVGQAQLQQNLWLLAYFVVLVVSVVDWIVSLSLNCEESLRVRRLLRPFFLLQNSSMMKKTLKCIRWSLPEMASVGLLLTIHLCLFTVIGMLLFTIAEKDEAQNKERLTYFRNLPEALTSLLVLLTTSNNPDGSLFLMNLLTAIIYNQFRGYLMKSLQTSLFRRRLGARAAYEVLTLQAGPNGTTSELVGVNPENFLRVLQKTQLNKIHKQAIIQKVHSYEGRPILADEFQKLFDEVDKGIVKERPPKPQYQSPFLQTAQFIFSHRYFDYLGNLIALGNLLSICVFLVLDSNLLPGERNDFVLGILNYIFILYYLLELLFKVFALGLRGYLFYHSNVFDGLLTIILLVLEISTLAVYRLPHPGWKPEQYGPLSLWDMTRLVNILIVIRFLRIIPSIKPTAVVASTILGLIQNLRAFGGILVVVYYVFAMIGINLFRGVIVPPGNSSLVPDNSSAPCGSFEQLGYWPNNFDDFAAALITLWDVMVVNNWQVILEAYKHYSGPWSMVYFVLWWLVSSVIWINLFLALLLENFLHRWDPQGHKQLLVGTKQITYEMSVELMFRDILEEPKEEELMEKLHKHPHLHLCR

Target ORF information:

RefSeq Version	NM_001107566.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus two pore segment channel 2 (Tpcn2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001107566.1

ORF Insert Sequence:

ATGGCGGCAG AAGCGCAGCC CCTTCTGGGC CGGGACCGAG GTGGTGGCCA GGCCCACTCT 
GGCGCTGATC AGGAGCTCTG CATAGACCAG GCTGTGGTCT TCATTGAAGA TGCCATAAAG 
TACCGATCCA TCTACCACCG CCTGGACGCT GGCTCACTGT GGCTTTACCG CTGGTATTAC 
TCGAACGTGT GCCAACGAGT GCTGGGCTTC ACCATTTTCC TGATCCTGGC CTTGGCTTTT 
GTGGAGGTCC CATCTTCCTT CACAAGGACT GCGGATGTGC GCTACCGCTC CCAGCCCTGG 
CAGCCACCCT GTGGCCTGAC AGAGACAATT GAGGCGCTCT GCTTGCTGGT CTTCCTGGCT 
GACCTCTCTG TGAAGGGCTA CCTGGTGGGG CAGGCCCAGT TGCAGCAGAA CCTGTGGCTG 
CTGGCTTACT TTGTGGTGCT GGTTGTGTCT GTGGTGGACT GGATCGTTTC GCTGAGCCTC 
AACTGTGAGG AGTCTCTGCG GGTGCGCCGG CTACTCCGAC CCTTCTTCCT GCTGCAGAAT 
TCCTCCATGA TGAAGAAGAC CCTGAAATGT ATACGGTGGT CACTACCAGA AATGGCTAGT 
GTTGGGCTGC TGCTGACTAT CCACCTGTGT CTCTTCACCG TAATCGGGAT GCTGCTGTTC 
ACCATTGCTG AGAAGGATGA AGCACAGAAC AAGGAGAGGC TGACCTACTT CCGGAACCTT 
CCAGAAGCAC TGACCTCACT CCTAGTGCTG CTGACTACTT CCAACAACCC TGATGGAAGC 
TTGTTTCTGA TGAACCTGCT GACGGCCATC ATCTATAATC AGTTCCGAGG CTACCTAATG 
AAATCTCTAC AGACTTCACT GTTCCGGCGG CGACTAGGGG CCCGTGCGGC CTATGAAGTC 
CTCACCCTTC AGGCAGGGCC AAATGGAACC ACCTCTGAGT TAGTCGGGGT AAATCCCGAG 
AACTTCCTGC GAGTGCTTCA GAAAACCCAG CTGAACAAAA TCCACAAACA AGCCATCATA 
CAGAAGGTGC ACTCCTATGA AGGCCGCCCG ATCCTGGCCG ATGAGTTCCA GAAACTCTTT 
GATGAGGTTG ACAAAGGTAT AGTCAAAGAG CGCCCGCCGA AGCCCCAGTA CCAGTCTCCG 
TTTCTGCAGA CTGCCCAGTT CATCTTCAGC CACCGCTACT TTGACTACCT GGGGAACCTC 
ATTGCTCTGG GAAACCTCTT GTCTATTTGT GTGTTCCTGG TGCTGGACTC AAACCTGCTG 
CCTGGGGAAC GGAACGACTT TGTCCTGGGG ATTCTCAACT ACATTTTCAT CTTGTACTAC 
CTGCTGGAGC TGCTGTTCAA GGTGTTCGCG CTGGGCCTAC GGGGCTACCT GTTCTACCAC 
AGCAACGTGT TCGATGGCCT CCTGACCATC ATCCTGCTGG TTTTGGAGAT TTCTACTCTG 
GCCGTGTACC GATTGCCGCA CCCAGGATGG AAGCCAGAGC AGTATGGCCC CCTGTCGCTT 
TGGGACATGA CTCGGCTGGT GAACATTCTG ATTGTGATAC GCTTCCTGCG CATCATCCCC 
AGCATAAAGC CAACGGCTGT GGTAGCCAGC ACCATCCTGG GCCTAATCCA GAACCTGCGG 
GCATTTGGTG GGATCCTGGT GGTGGTATAC TATGTGTTCG CCATGATTGG GATCAACCTG 
TTCCGAGGTG TCATTGTGCC TCCCGGAAAC AGCAGCCTGG TTCCTGATAA CAGCTCAGCT 
CCGTGTGGAA GCTTTGAGCA GCTAGGTTAC TGGCCCAACA ACTTCGACGA CTTTGCTGCT 
GCTCTGATCA CACTGTGGGA CGTGATGGTG GTGAACAATT GGCAGGTGAT CCTGGAAGCC 
TATAAGCACT ACTCCGGCCC GTGGTCGATG GTGTATTTTG TGCTGTGGTG GTTGGTGTCC 
TCTGTCATCT GGATCAACCT GTTTCTGGCT CTGCTTCTGG AGAACTTTCT CCACAGATGG 
GACCCTCAAG GTCATAAGCA GCTCCTGGTT GGGACCAAGC AGATCACCTA CGAGATGTCT 
GTGGAGCTCA TGTTCAGAGA TATCCTAGAA GAGCCCAAGG AGGAGGAACT GATGGAGAAG 
CTACACAAGC ATCCACACTT GCATCTGTGC AGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Tpcn2 ( XM_006230784.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006230846.1 Rattus norvegicus two pore segment channel 2 (Tpcn2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa23761
Clone ID Related Accession (Same CDS sequence)	XM_006230784.3
Accession Version	XM_006230784.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2202bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	two pore calcium channel protein 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006230784.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCAG AAGCGCAGCC CCTTCTGGGC CGGGACCGAG GTGGTGGCCA GGCCCACTCT 
GGCGCTGATC AGGAGCTCTG CATAGACCAG GCTGTGGTCT TCATTGAAGA TGCCATAAAG 
TACCGATCCA TCTACCACCG CCTGGACGCT GGCTCACTGT GGCTTTACCG CTGGTATTAC 
TCGAACGTGT GCCAACGAGT GCTGGGCTTC ACCATTTTCC TGATCCTGGC CTTGGCTTTT 
GTGGAGGTCC CATCTTCCTT CACAAGGACT GCGGATGTGC GCTACCGCTC CCAGCCCTGG 
CAGCCACCCT GTGGCCTGAC AGAGACAATT GAGGCGCTCT GCTTGCTGGT CTTCCTGGCT 
GACCTCTCTG TGAAGGGCTA CCTGGTGGGG CAGGCCCAGT TGCAGCAGAA CCTGTGGCTG 
CTGGCTTACT TTGTGGTGCT GGTTGTGTCT GTGGTGGACT GGATCGTTTC GCTGAGCCTC 
AACTGTGAGG AGTCTCTGCG GGTGCGCCGG CTACTCCGAC CCTTCTTCCT GCTGCAGAAT 
TCCTCCATGA TGAAGAAGAC CCTGAAATGT ATACGGTGGT CACTACCAGA AATGGCTAGT 
GTTGGGCTGC TGCTGACTAT CCACCTGTGT CTCTTCACCG TAATCGGGAT GCTGCTGTTC 
ACCATTGCTG AGAAGGATGA AGCACAGAAC AAGGAGAGGC TGACCTACTT CCGGAACCTT 
CCAGAAGCAC TGACCTCACT CCTAGTGCTG CTGACTACTT CCAACAACCC TGATGTGATG 
ATCCCTGCGT ATACCCAGAA CCGGGCCTAT GCTCTCTTCT TCATAATCTT CACCTTGATA 
GGAAGCTTGT TTCTGATGAA CCTGCTGACG GCCATCATCT ATAATCAGTT CCGAGGCTAC 
CTAATGAAAT CTCTACAGAC TTCACTGTTC CGGCGGCGAC TAGGGGCCCG TGCGGCCTAT 
GAAGTCCTCA CCCTTCAGGC AGGGCCAAAT GGAACCACCT CTGAGTTAGT CGGGGTAAAT 
CCCGAGAACT TCCTGCGAGT GCTTCAGAAA ACCCAGCTGA ACAAAATCCA CAAACAAGCC 
ATCATACAGA AGGTGCACTC CTATGAAGGC CGCCCGATCC TGGCCGATGA GTTCCAGAAA 
CTCTTTGATG AGGTTGACAA AGGTATAGTC AAAGAGCGCC CGCCGAAGCC CCAGTACCAG 
TCTCCGTTTC TGCAGACTGC CCAGTTCATC TTCAGCCACC GCTACTTTGA CTACCTGGGG 
AACCTCATTG CTCTGGGAAA CCTCTTGTCT ATTTGTGTGT TCCTGGTGCT GGACTCAAAC 
CTGCTGCCTG GGGAACGGAA CGACTTTGTC CTGGGGATTC TCAACTACAT TTTCATCTTG 
TACTACCTGC TGGAGCTGCT GTTCAAGGTG TTCGCGCTGG GCCTACGGGG CTACCTGTTC 
TACCACAGCA ACGTGTTCGA TGGCCTCCTG ACCATCATCC TGCTGGTTTT GGAGATTTCT 
ACTCTGGCCG TGTACCGATT GCCGCACCCA GGATGGAAGC CAGAGCAGTA TGGCCCCCTG 
TCGCTTTGGG ACATGACTCG GCTGGTGAAC ATTCTGATTG TGATACGCTT CCTGCGCATC 
ATCCCCAGCA TAAAGCCAAC GGCTGTGGTA GCCAGCACCA TCCTGGGCCT AATCCAGAAC 
CTGCGGGCAT TTGGTGGGAT CCTGGTGGTG GTATACTATG TGTTCGCCAT GATTGGGATC 
AACCTGTTCC GAGGTGTCAT TGTGCCTCCC GGAAACAGCA GCCTGGTTCC TGATAACAGC 
TCAGCTCCGT GTGGAAGCTT TGAGCAGCTA GGTTACTGGC CCAACAACTT CGACGACTTT 
GCTGCTGCTC TGATCACACT GTGGGACGTG ATGGTGGTGA ACAATTGGCA GGTGATCCTG 
GAAGCCTATA AGCACTACTC CGGCCCGTGG TCGATGGTGT ATTTTGTGCT GTGGTGGTTG 
GTGTCCTCTG TCATCTGGAT CAACCTGTTT CTGGCTCTGC TTCTGGAGAA CTTTCTCCAC 
AGATGGGACC CTCAAGGTCA TAAGCAGCTC CTGGTTGGGA CCAAGCAGAT CACCTACGAG 
ATGTCTGTGG AGCTCATGTT CAGAGATATC CTAGAAGAGC CCAAGGAGGA GGAACTGATG 
GAGAAGCTAC ACAAGCATCC ACACTTGCAT CTGTGCAGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006230846.1
CDS	106..2307
Translation	MAAEAQPLLGRDRGGGQAHSGADQELCIDQAVVFIEDAIKYRSIYHRLDAGSLWLYRWYYSNVCQRVLGFTIFLILALAFVEVPSSFTRTADVRYRSQPWQPPCGLTETIEALCLLVFLADLSVKGYLVGQAQLQQNLWLLAYFVVLVVSVVDWIVSLSLNCEESLRVRRLLRPFFLLQNSSMMKKTLKCIRWSLPEMASVGLLLTIHLCLFTVIGMLLFTIAEKDEAQNKERLTYFRNLPEALTSLLVLLTTSNNPDVMIPAYTQNRAYALFFIIFTLIGSLFLMNLLTAIIYNQFRGYLMKSLQTSLFRRRLGARAAYEVLTLQAGPNGTTSELVGVNPENFLRVLQKTQLNKIHKQAIIQKVHSYEGRPILADEFQKLFDEVDKGIVKERPPKPQYQSPFLQTAQFIFSHRYFDYLGNLIALGNLLSICVFLVLDSNLLPGERNDFVLGILNYIFILYYLLELLFKVFALGLRGYLFYHSNVFDGLLTIILLVLEISTLAVYRLPHPGWKPEQYGPLSLWDMTRLVNILIVIRFLRIIPSIKPTAVVASTILGLIQNLRAFGGILVVVYYVFAMIGINLFRGVIVPPGNSSLVPDNSSAPCGSFEQLGYWPNNFDDFAAALITLWDVMVVNNWQVILEAYKHYSGPWSMVYFVLWWLVSSVIWINLFLALLLENFLHRWDPQGHKQLLVGTKQITYEMSVELMFRDILEEPKEEELMEKLHKHPHLHLCR

Target ORF information:

RefSeq Version	XM_006230784.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus two pore segment channel 2 (Tpcn2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006230784.3

ORF Insert Sequence:

ATGGCGGCAG AAGCGCAGCC CCTTCTGGGC CGGGACCGAG GTGGTGGCCA GGCCCACTCT 
GGCGCTGATC AGGAGCTCTG CATAGACCAG GCTGTGGTCT TCATTGAAGA TGCCATAAAG 
TACCGATCCA TCTACCACCG CCTGGACGCT GGCTCACTGT GGCTTTACCG CTGGTATTAC 
TCGAACGTGT GCCAACGAGT GCTGGGCTTC ACCATTTTCC TGATCCTGGC CTTGGCTTTT 
GTGGAGGTCC CATCTTCCTT CACAAGGACT GCGGATGTGC GCTACCGCTC CCAGCCCTGG 
CAGCCACCCT GTGGCCTGAC AGAGACAATT GAGGCGCTCT GCTTGCTGGT CTTCCTGGCT 
GACCTCTCTG TGAAGGGCTA CCTGGTGGGG CAGGCCCAGT TGCAGCAGAA CCTGTGGCTG 
CTGGCTTACT TTGTGGTGCT GGTTGTGTCT GTGGTGGACT GGATCGTTTC GCTGAGCCTC 
AACTGTGAGG AGTCTCTGCG GGTGCGCCGG CTACTCCGAC CCTTCTTCCT GCTGCAGAAT 
TCCTCCATGA TGAAGAAGAC CCTGAAATGT ATACGGTGGT CACTACCAGA AATGGCTAGT 
GTTGGGCTGC TGCTGACTAT CCACCTGTGT CTCTTCACCG TAATCGGGAT GCTGCTGTTC 
ACCATTGCTG AGAAGGATGA AGCACAGAAC AAGGAGAGGC TGACCTACTT CCGGAACCTT 
CCAGAAGCAC TGACCTCACT CCTAGTGCTG CTGACTACTT CCAACAACCC TGATGTGATG 
ATCCCTGCGT ATACCCAGAA CCGGGCCTAT GCTCTCTTCT TCATAATCTT CACCTTGATA 
GGAAGCTTGT TTCTGATGAA CCTGCTGACG GCCATCATCT ATAATCAGTT CCGAGGCTAC 
CTAATGAAAT CTCTACAGAC TTCACTGTTC CGGCGGCGAC TAGGGGCCCG TGCGGCCTAT 
GAAGTCCTCA CCCTTCAGGC AGGGCCAAAT GGAACCACCT CTGAGTTAGT CGGGGTAAAT 
CCCGAGAACT TCCTGCGAGT GCTTCAGAAA ACCCAGCTGA ACAAAATCCA CAAACAAGCC 
ATCATACAGA AGGTGCACTC CTATGAAGGC CGCCCGATCC TGGCCGATGA GTTCCAGAAA 
CTCTTTGATG AGGTTGACAA AGGTATAGTC AAAGAGCGCC CGCCGAAGCC CCAGTACCAG 
TCTCCGTTTC TGCAGACTGC CCAGTTCATC TTCAGCCACC GCTACTTTGA CTACCTGGGG 
AACCTCATTG CTCTGGGAAA CCTCTTGTCT ATTTGTGTGT TCCTGGTGCT GGACTCAAAC 
CTGCTGCCTG GGGAACGGAA CGACTTTGTC CTGGGGATTC TCAACTACAT TTTCATCTTG 
TACTACCTGC TGGAGCTGCT GTTCAAGGTG TTCGCGCTGG GCCTACGGGG CTACCTGTTC 
TACCACAGCA ACGTGTTCGA TGGCCTCCTG ACCATCATCC TGCTGGTTTT GGAGATTTCT 
ACTCTGGCCG TGTACCGATT GCCGCACCCA GGATGGAAGC CAGAGCAGTA TGGCCCCCTG 
TCGCTTTGGG ACATGACTCG GCTGGTGAAC ATTCTGATTG TGATACGCTT CCTGCGCATC 
ATCCCCAGCA TAAAGCCAAC GGCTGTGGTA GCCAGCACCA TCCTGGGCCT AATCCAGAAC 
CTGCGGGCAT TTGGTGGGAT CCTGGTGGTG GTATACTATG TGTTCGCCAT GATTGGGATC 
AACCTGTTCC GAGGTGTCAT TGTGCCTCCC GGAAACAGCA GCCTGGTTCC TGATAACAGC 
TCAGCTCCGT GTGGAAGCTT TGAGCAGCTA GGTTACTGGC CCAACAACTT CGACGACTTT 
GCTGCTGCTC TGATCACACT GTGGGACGTG ATGGTGGTGA ACAATTGGCA GGTGATCCTG 
GAAGCCTATA AGCACTACTC CGGCCCGTGG TCGATGGTGT ATTTTGTGCT GTGGTGGTTG 
GTGTCCTCTG TCATCTGGAT CAACCTGTTT CTGGCTCTGC TTCTGGAGAA CTTTCTCCAC 
AGATGGGACC CTCAAGGTCA TAAGCAGCTC CTGGTTGGGA CCAAGCAGAT CACCTACGAG 
ATGTCTGTGG AGCTCATGTT CAGAGATATC CTAGAAGAGC CCAAGGAGGA GGAACTGATG 
GAGAAGCTAC ACAAGCATCC ACACTTGCAT CTGTGCAGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Dysregulation of lysosomal morphology by pathogenic LRRK2 is corrected by TPC2 inhibition.
Journal of cell science128(2)232-8(2015 Jan)
Hockey LN,Kilpatrick BS,Eden ER,Lin-Moshier Y,Brailoiu GC,Brailoiu E,Futter CE,Schapira AH,Marchant JS,Patel S

Identification of a novel gene for diabetic traits in rats, mice, and humans.
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Tpcn2 cDNA ORF clone, Rattus norvegicus(Norway rat)

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