gRNAs
The following Dennd5a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Dennd5a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.
| CloneID | ORa56611 | |
| Clone ID Related Accession (Same CDS sequence) | XM_017589204.1 | |
| Accession Version | XM_017589204.1 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 3792bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector |  User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2016-07-25 | |
| Organism | Rattus norvegicus(Norway rat) | |
| Product | DENN domain-containing protein 5A isoform X1 | |
| Comment | Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## | |
1 | ATGAGCGGCG GCGGCGGCGG AGGGGGCTCG GCCCCCAGTC GCTTCGCCGA CTATTTTGTC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | XP_017444693.1 |
| CDS | 265..4056 |
| Translation |
Target ORF information:
Target ORF information:
|
 XM_017589204.1 |
1 | ATGAGCGGCG GCGGCGGCGG AGGGGGCTCG GCCCCCAGTC GCTTCGCCGA CTATTTTGTC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| CloneID | ORa03922 | |
| Clone ID Related Accession (Same CDS sequence) | NM_001107546.2 | |
| Accession Version | NM_001107546.2 Latest version! | Documents for ORF clone product in default vector |
| Sequence Information | ORF Nucleotide Sequence (Length: 3864bp) Protein sequence SNP |
|
| Vector | pcDNA3.1-C-(k)DYK or customized vector | User Manual |
| Clone information | Clone Map | MSDS |
| Tag on pcDNA3.1+/C-(K)DYK | C terminal DYKDDDDK tags | |
| ORF Insert Method | CloneEZ™ Seamless cloning technology | |
| Insert Structure | linear | |
| Update Date | 2019-10-18 | |
| Organism | Rattus norvegicus(Norway rat) | |
| Product | DENN domain-containing protein 5A | |
| Comment | Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC098265.5 and AABR07071965.1. On Apr 18, 2009 this sequence version replaced NM_001107546.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END## | |
1 | ATGAGCGGCG GCGGCGGCGG AGGGGGCTCG GCCCCCAGTC GCTTCGCCGA CTATTTTGTC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
| RefSeq | NP_001101016.2 |
| CDS | 241..4104 |
| Translation |
Target ORF information:
Target ORF information:
|
NM_001107546.2 |
1 | ATGAGCGGCG GCGGCGGCGG AGGGGGCTCG GCCCCCAGTC GCTTCGCCGA CTATTTTGTC |
The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.
Epileptic Encephalopathy Caused by Mutations in the Guanine Nucleotide Exchange Factor DENND5A. |
Family-wide characterization of the DENN domain Rab GDP-GTP exchange factors. |
The retromer coat complex coordinates endosomal sorting and dynein-mediated transport, with carrier recognition by the trans-Golgi network. |
Structural basis for recruitment of Rab6-interacting protein 1 to Golgi via a RUN domain. |
Common sequence and structural features in the heat-shock factor and Ets families of DNA-binding domains. |
