Cyfip1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Cyfip1
Entrez Gene ID	308666
Full Name	cytoplasmic FMR1 interacting protein 1
General protein information	Preferred Names cytoplasmic FMR1 interacting protein 1 Names cytoplasmic FMR1-interacting protein 1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 1 Map Location: 1q22

mRNA and Protein(s)

mRNA	Protein	Name
XM_006229288.2	XP_006229350.1	cytoplasmic FMR1-interacting protein 1 isoform X1
NM_001107517.1	NP_001100987.1	cytoplasmic FMR1-interacting protein 1
XM_017589152.1	XP_017444641.1	cytoplasmic FMR1-interacting protein 1 isoform X2

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	CYFIP1	XP_003640571.1
Homo sapiens (human)	CYFIP1	NP_055423.1
Pan troglodytes (chimpanzee)	CYFIP1	XP_003314612.1
Mus musculus (house mouse)	Cyfip1	NP_035500.2
Xenopus tropicalis (tropical clawed frog)	cyfip1	XP_004911869.1
Danio rerio (zebrafish)	cyfip1	NP_997924.1
Caenorhabditis elegans (roundworm)	gex-2	NP_499949.2
	CYFIP1	XP_002804722.1
Bos taurus (cattle)	CYFIP1	NP_001192444.1
Rattus norvegicus (Norway rat)	Cyfip1	NP_001100987.1

Gene Ontology
Bibliography

Related articles in PubMed

Retrolinkin recruits the WAVE1 protein complex to facilitate BDNF-induced TrkB endocytosis and dendrite outgrowth.
Xu C, Fu X, Zhu S, Liu JJ
Molecular biology of the cell27(21)3342-3356(2016 Nov)

Cyfip1 Regulates Presynaptic Activity during Development.
Hsiao K, Harony-Nicolas H, Buxbaum JD, Bozdagi-Gunal O, Benson DL
The Journal of neuroscience : the official journal of the Society for Neuroscience36(5)1564-76(2016 Feb)

Up-regulated cytoplasmic FMRP-interacting protein 1 in intractable temporal lobe epilepsy patients and a rat model.
Huang Y
The International journal of neuroscience1-10(2015 Aug)

Two-stage translational control of dentate gyrus LTP consolidation is mediated by sustained BDNF-TrkB signaling to MNK.
Panja D, Kenney JW, D'Andrea L, Zalfa F, Vedeler A, Wibrand K, Fukunaga R, Bagni C, Proud CG, Bramham CR
Cell reports9(4)1430-45(2014 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Dysregulation of CYFIP1 expression levels leads to pathological changes in CNS maturation and neuronal connectivity, both of which may contribute to the development of the neurological symptoms seen in ASD and SCZ.
Title: The autism and schizophrenia associated gene CYFIP1 is critical for the maintenance of dendritic complexity and the stabilization of mature spines.

The following Cyfip1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Cyfip1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa56584	XM_017589152.1 Latest version!	Rattus norvegicus cytoplasmic FMR1 interacting protein 1 (Cyfip1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
ORa00157	NM_001107517.1 Latest version!	Rattus norvegicus cytoplasmic FMR1 interacting protein 1 (Cyfip1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$923.30 $1319.00
ORa22853	XM_006229288.2 Latest version!	Rattus norvegicus cytoplasmic FMR1 interacting protein 1 (Cyfip1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00

ORF Online Only Promotion

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Cyfip1 ( XM_017589152.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017444641.1 Rattus norvegicus cytoplasmic FMR1 interacting protein 1 (Cyfip1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa56584
Clone ID Related Accession (Same CDS sequence)	XM_017589152.1
Accession Version	XM_017589152.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2082bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	cytoplasmic FMR1-interacting protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGAGAA CTATGCTAGA GTCCCTCATT GCAGACAAAA GTGGTTCCAA GAAAACCTTG 
AGAAGTAGCC TTGAGGGGCC CACCATATTG GACATAGAAA AATTTCATCG AGAATCATTC 
TTCTACACTC ACTTGATAAA TTTCAGTGAA ACACTGCAGC AGTGCTGTGA CCTTTCCCAG 
CTGTGGTTCC GAGAGTTCTT CCTGGAGCTG ACCATGGGCA GGAGGATCCA GTTCCCCATT 
GAGATGTCTA TGCCCTGGAT TCTGACTGAC CACATCCTAG AGACCAAGGA GGCATCAATG 
ATGGAGTACG TCCTCTACTC CTTGGACCTG TACAATGACA GTGCTCACTA TGCGCTCACC 
AAGTTCAACA AGCAGTTTCT CTATGATGAA ATTGAAGCAG AGGTGAATCT GTGTTTTGAC 
CAATTTGTTT ATAAACTGGC AGACCAGATA TTTGCATATT ACAAGGTTAT GGCAGGAAGT 
TTGCTTCTTG ACAAACGGTT ACGATCAGAA TGCAAAAATC AGGGAGCCAC GATTCACCTC 
CCACCGTCTA ACCGCTATGA GACGTTGTTG AAGCAAAGGC ACGTGCAGCT GCTTGGGAGA 
TCCATCGATC TCAATCGTCT GATTACACAG CGTGTTTCAG CAGCCATGTA CAAATCTCTA 
GAACTGGCAA TTGGACGATT TGAAAGTGAA GATTTAACAT CAGTAGTCGA GCTAGATGGG 
CTCTTAGAAA TCAACCGTAT GACGCACAAG CTGCTGAGTA GGTACCTCAC ACTAGACAGC 
TTTGATGCCA TGTTCCGGGA AGCCAACCAC AACGTGTCTG CACCTTATGG AAGAATCACC 
CTCCATGTCT TCTGGGAGCT AAACTATGAC TTCTTGCCCA ACTACTGCTA TAATGGCTCC 
ACCAACCGGT TTGTTCGGAC AGTACTACCA TTTTCTCAGG AATTTCAACG AGACAAACAA 
CCTAATGCAC AGCCCCAGTA TTTGCACGGC TCCAAGGCTT TGAACCTAGC CTACTCAAGC 
ATTTATGGCA GCTACCGGAA CTTTGTGGGG CCTCCTCACT TCCAGGTCAT CTGCCGGCTG 
CTTGGCTACC AGGGCATTGC AGTCGTCATG GAAGAACTGC TGAAGGTTGT CAAGAGCCTG 
CTTCAAGGCA CAATCCTGCA GTATGTGAAA ACTCTGATGG AAGTGATGCC TAAGATCTGC 
CGCCTTCCCC GACATGAGTA CGGCTCTCCT GGCATCCTAG AGTTCTTCCA CCACCAGCTG 
AAGGACATTG TGGAGTATGC AGAGCTGAAA ACAGTATGCT TCCAGAACCT GCGGGAGGTG 
GGAAATGCTG TTCTCTTCTG CCTGCTCATC GAGCAAAGCC TGTCTTTAGA AGAAGTCTGT 
GACCTGTTGC ATGCAGCTCC TTTTCAGAAT ATCCTGCCTC GAGTCCACGT AAAAGAGGGG 
GAGAGAGTTG ATGCCAAAAT GAAAAGACTA GAATCCAAGT ATGCTCCACT GCATCTTGTT 
CCGCTGATTG AAAGGCTGGG GACCCCACAG CAAATTGCAA TCGCAAGAGA GGGGGACTTG 
CTGACCAAGG AGCGGCTCTG TTGTGGTCTG TCCATGTTTG AAGTCATCCT CACACGGATC 
CGGACCTTTC TGGATGATCC CATCTGGCGT GGGCCCCTGC CCAGCAATGG GGTCATGCAT 
GTGGATGAGT GTGTGGAGTT TCACAGACTA TGGAGTGCCA TGCAGTTTGT TTACTGCATT 
CCTGTAGGGA CACACGAGTT TACAGTGGAG CAGTGTTTTG GCGATGGGCT CCACTGGGCT 
GGCTGCATGA TCATTGTACT TCTGGGACAG CAGCGGCGCT TTGCTGTGTT GGATTTCTGC 
TATCATCTTC TCAAAGTTCA GAAACACGAT GGCAAGGATG AGATCATCAA AAATGTGCCA 
TTGAAGAAGA TGGTGGAGAG GATTCGCAAG TTCCAGATTC TCAATGATGA GATCATCACT 
ATCCTGGACA AGTACTTGAA ATCTGGAGAT GGGGAGAGCA CACCTGTGGA ACATGTACGC 
TGCTTCCAGC CACCCATCCA CCAGTCCCTA GCCAGCAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017444641.1
CDS	96..2177
Translation	MVRTMLESLIADKSGSKKTLRSSLEGPTILDIEKFHRESFFYTHLINFSETLQQCCDLSQLWFREFFLELTMGRRIQFPIEMSMPWILTDHILETKEASMMEYVLYSLDLYNDSAHYALTKFNKQFLYDEIEAEVNLCFDQFVYKLADQIFAYYKVMAGSLLLDKRLRSECKNQGATIHLPPSNRYETLLKQRHVQLLGRSIDLNRLITQRVSAAMYKSLELAIGRFESEDLTSVVELDGLLEINRMTHKLLSRYLTLDSFDAMFREANHNVSAPYGRITLHVFWELNYDFLPNYCYNGSTNRFVRTVLPFSQEFQRDKQPNAQPQYLHGSKALNLAYSSIYGSYRNFVGPPHFQVICRLLGYQGIAVVMEELLKVVKSLLQGTILQYVKTLMEVMPKICRLPRHEYGSPGILEFFHHQLKDIVEYAELKTVCFQNLREVGNAVLFCLLIEQSLSLEEVCDLLHAAPFQNILPRVHVKEGERVDAKMKRLESKYAPLHLVPLIERLGTPQQIAIAREGDLLTKERLCCGLSMFEVILTRIRTFLDDPIWRGPLPSNGVMHVDECVEFHRLWSAMQFVYCIPVGTHEFTVEQCFGDGLHWAGCMIIVLLGQQRRFAVLDFCYHLLKVQKHDGKDEIIKNVPLKKMVERIRKFQILNDEIITILDKYLKSGDGESTPVEHVRCFQPPIHQSLASS

Target ORF information:

RefSeq Version	XM_017589152.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus cytoplasmic FMR1 interacting protein 1 (Cyfip1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017589152.1

ORF Insert Sequence:

ATGGTGAGAA CTATGCTAGA GTCCCTCATT GCAGACAAAA GTGGTTCCAA GAAAACCTTG 
AGAAGTAGCC TTGAGGGGCC CACCATATTG GACATAGAAA AATTTCATCG AGAATCATTC 
TTCTACACTC ACTTGATAAA TTTCAGTGAA ACACTGCAGC AGTGCTGTGA CCTTTCCCAG 
CTGTGGTTCC GAGAGTTCTT CCTGGAGCTG ACCATGGGCA GGAGGATCCA GTTCCCCATT 
GAGATGTCTA TGCCCTGGAT TCTGACTGAC CACATCCTAG AGACCAAGGA GGCATCAATG 
ATGGAGTACG TCCTCTACTC CTTGGACCTG TACAATGACA GTGCTCACTA TGCGCTCACC 
AAGTTCAACA AGCAGTTTCT CTATGATGAA ATTGAAGCAG AGGTGAATCT GTGTTTTGAC 
CAATTTGTTT ATAAACTGGC AGACCAGATA TTTGCATATT ACAAGGTTAT GGCAGGAAGT 
TTGCTTCTTG ACAAACGGTT ACGATCAGAA TGCAAAAATC AGGGAGCCAC GATTCACCTC 
CCACCGTCTA ACCGCTATGA GACGTTGTTG AAGCAAAGGC ACGTGCAGCT GCTTGGGAGA 
TCCATCGATC TCAATCGTCT GATTACACAG CGTGTTTCAG CAGCCATGTA CAAATCTCTA 
GAACTGGCAA TTGGACGATT TGAAAGTGAA GATTTAACAT CAGTAGTCGA GCTAGATGGG 
CTCTTAGAAA TCAACCGTAT GACGCACAAG CTGCTGAGTA GGTACCTCAC ACTAGACAGC 
TTTGATGCCA TGTTCCGGGA AGCCAACCAC AACGTGTCTG CACCTTATGG AAGAATCACC 
CTCCATGTCT TCTGGGAGCT AAACTATGAC TTCTTGCCCA ACTACTGCTA TAATGGCTCC 
ACCAACCGGT TTGTTCGGAC AGTACTACCA TTTTCTCAGG AATTTCAACG AGACAAACAA 
CCTAATGCAC AGCCCCAGTA TTTGCACGGC TCCAAGGCTT TGAACCTAGC CTACTCAAGC 
ATTTATGGCA GCTACCGGAA CTTTGTGGGG CCTCCTCACT TCCAGGTCAT CTGCCGGCTG 
CTTGGCTACC AGGGCATTGC AGTCGTCATG GAAGAACTGC TGAAGGTTGT CAAGAGCCTG 
CTTCAAGGCA CAATCCTGCA GTATGTGAAA ACTCTGATGG AAGTGATGCC TAAGATCTGC 
CGCCTTCCCC GACATGAGTA CGGCTCTCCT GGCATCCTAG AGTTCTTCCA CCACCAGCTG 
AAGGACATTG TGGAGTATGC AGAGCTGAAA ACAGTATGCT TCCAGAACCT GCGGGAGGTG 
GGAAATGCTG TTCTCTTCTG CCTGCTCATC GAGCAAAGCC TGTCTTTAGA AGAAGTCTGT 
GACCTGTTGC ATGCAGCTCC TTTTCAGAAT ATCCTGCCTC GAGTCCACGT AAAAGAGGGG 
GAGAGAGTTG ATGCCAAAAT GAAAAGACTA GAATCCAAGT ATGCTCCACT GCATCTTGTT 
CCGCTGATTG AAAGGCTGGG GACCCCACAG CAAATTGCAA TCGCAAGAGA GGGGGACTTG 
CTGACCAAGG AGCGGCTCTG TTGTGGTCTG TCCATGTTTG AAGTCATCCT CACACGGATC 
CGGACCTTTC TGGATGATCC CATCTGGCGT GGGCCCCTGC CCAGCAATGG GGTCATGCAT 
GTGGATGAGT GTGTGGAGTT TCACAGACTA TGGAGTGCCA TGCAGTTTGT TTACTGCATT 
CCTGTAGGGA CACACGAGTT TACAGTGGAG CAGTGTTTTG GCGATGGGCT CCACTGGGCT 
GGCTGCATGA TCATTGTACT TCTGGGACAG CAGCGGCGCT TTGCTGTGTT GGATTTCTGC 
TATCATCTTC TCAAAGTTCA GAAACACGAT GGCAAGGATG AGATCATCAA AAATGTGCCA 
TTGAAGAAGA TGGTGGAGAG GATTCGCAAG TTCCAGATTC TCAATGATGA GATCATCACT 
ATCCTGGACA AGTACTTGAA ATCTGGAGAT GGGGAGAGCA CACCTGTGGA ACATGTACGC 
TGCTTCCAGC CACCCATCCA CCAGTCCCTA GCCAGCAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cyfip1 ( NM_001107517.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001100987.1 Rattus norvegicus cytoplasmic FMR1 interacting protein 1 (Cyfip1), mRNA.

Price & Availability↑

CloneID	ORa00157
Clone ID Related Accession (Same CDS sequence)	NM_001107517.1
Accession Version	NM_001107517.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3762bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-15
Organism	Rattus norvegicus(Norway rat)
Product	cytoplasmic FMR1-interacting protein 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CH474036.2. On or before Oct 4, 2007 this sequence version replaced XM_218717.4, XM_001054108.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGCTGCCC AAGTGACACT AGAGGACGCA CTGTCCAATG TGGACCTCCT GGAAGAGCTG 
CCTCTTCCTG ACCAGCAGCC CTGCATTGAG CCCCCACCTT CCTCGCTGCT CTATCAGCCA 
AATTTCAACA CCAACTTTGA AGACCGAAAT GCATTTGTCA CTGGCATTGC AAGATACATT 
GAACAAGCAA CTGTCCACTC TAGCATGAAC GAGATGCTAG AGGAAGGCCA AGAGTATGCT 
GTCATGCTGT ATACCTGGAG GAGCTGCTCC CGTGCCATCC CTCAGGTGAA GTGCAATGAG 
CAGCCTAATA GGGTTGAAAT TTATGAGAAA ACTGTGGAAG TCCTTGAACC CGAGGTCACA 
AAACTGATGA ATTTTATGTA CTTCCAGAGA AATGCCATCG AGCGTTTCTG TGGGGAGGTG 
AGACGCCTGT GTCATGCTGA GAGGAGAAAG GACTTCGTGT CTGAAGCCTA CTTGATCACC 
TTGGGCAAGT TTATCAACAT GTTTGCTGTG TTGGATGAGC TGAAGAACAT GAAGTGCAGT 
GTGAAGAATG ACCACTCAGC GTATAAGAGG GCTGCTCAGT TTTTACGTAA AATGGCAGAT 
CCACAATCCA TCCAGGAGTC ACAGAATTTG TCCATGTTCC TGGCCAACCA CAACAAGATT 
ACACAATCTC TACAGCAACA GCTTGAGGTC ATTTCTGGCT ATGAAGAACT CCTGGCAGAC 
ATAGTGAATC TGTGTGTGGA CTACTATGAG AACAGAATGT ACTTGACGCC CAGCGAAAAA 
CACATGCTTC TCAAAGTCAT GGGATTTGGT CTGTACCTGA TGGATGGGAG TGTGAGTAAC 
ATCTACAAAC TAGATGCCAA GAAAAGAATA AATTTATCCA AAATAGACAA GTATTTCAAG 
CAGCTGCAGG TGGTTCCACT CTTTGGAGAT ATGCAGATAG AACTGGCAAG ATACATCAAG 
ACCAGCGCAC ACTATGAGGA GAATAAGTCC CGGTGGACCT GTACGTCCTC GAGCAGCAGC 
CCACAGTACA ACATCTGTGA GCAGATGATC CAGATCCGTG AGGACCACAT GCGTTTCATT 
TCTGAGCTGG CACGCTACAG CAATAGCGAG GTAGTCACAG GGTCTGGCCG GCAGGAGGCC 
CAGAAGACAG ATGCTGAGTA CCGGAAGCTC TTTGACCTGG CGCTGCAGGG CCTGCAGCTT 
CTGTCGCAGT GGAGCGCGCA TGTGATGGAA GTGTATTCCT GGAAACTTGT ACATCCAACA 
GACAAGTACT CCAACAAGGA CTGCCCAGAC AATGCTGAGG AGTATGAACG TGCCACGCGT 
TACAACTACA CCACTGAGGA GAAGTTTGCT CTGGTGGAGG TGATTGCCAT GATCAAAGGC 
CTGCAGGTGC TGATGGGCAG GATGGAGAGT GTGTTCAATC ACGCCATCAG ACACACAGTT 
TATGCTGCAC TTCAGGACTT CTCCCAGGTG ACCCTCAGGG AACCACTGCG ACAGGCCATC 
AAGAAGAAAA AGAATGTCAT CCAGAGTGTC CTACAGGCCA TCAGGAAGAC TGTGTGTGAC 
TGGGAGACTG GGCATGAACC CTTTAACGAC CCAGCTCTGC GGGGAGAGAA GGACCCTAAG 
AGCGGCTTTG ACATCAAGGT GCCACGCCGT GCTGTAGGAC CCTCCAGCAC ACAGCTTTAC 
ATGGTGAGAA CTATGCTAGA GTCCCTCATT GCAGACAAAA GTGGTTCCAA GAAAACCTTG 
AGAAGTAGCC TTGAGGGGCC CACCATATTG GACATAGAAA AATTTCATCG AGAATCATTC 
TTCTACACTC ACTTGATAAA TTTCAGTGAA ACACTGCAGC AGTGCTGTGA CCTTTCCCAG 
CTGTGGTTCC GAGAGTTCTT CCTGGAGCTG ACCATGGGCA GGAGGATCCA GTTCCCCATT 
GAGATGTCTA TGCCCTGGAT TCTGACTGAC CACATCCTAG AGACCAAGGA GGCATCAATG 
ATGGAGTACG TCCTCTACTC CTTGGACCTG TACAATGACA GTGCTCACTA TGCGCTCACC 
AAGTTCAACA AGCAGTTTCT CTATGATGAA ATTGAAGCAG AGGTGAATCT GTGTTTTGAC 
CAATTTGTTT ATAAACTGGC AGACCAGATA TTTGCATATT ACAAGGTTAT GGCAGGAAGT 
TTGCTTCTTG ACAAACGGTT ACGATCAGAA TGCAAAAATC AGGGAGCCAC GATTCACCTC 
CCACCGTCTA ACCGCTATGA GACGTTGTTG AAGCAAAGGC ACGTGCAGCT GCTTGGGAGA 
TCCATCGATC TCAATCGTCT GATTACACAG CGTGTTTCAG CAGCCATGTA CAAATCTCTA 
GAACTGGCAA TTGGACGATT TGAAAGTGAA GATTTAACAT CAGTAGTCGA GCTAGATGGG 
CTCTTAGAAA TCAACCGTAT GACGCACAAG CTGCTGAGTA GGTACCTCAC ACTAGACAGC 
TTTGATGCCA TGTTCCGGGA AGCCAACCAC AACGTGTCTG CACCTTATGG AAGAATCACC 
CTCCATGTCT TCTGGGAGCT AAACTATGAC TTCTTGCCCA ACTACTGCTA TAATGGCTCC 
ACCAACCGGT TTGTTCGGAC AGTACTACCA TTTTCTCAGG AATTTCAACG AGACAAACAA 
CCTAATGCAC AGCCCCAGTA TTTGCACGGC TCCAAGGCTT TGAACCTAGC CTACTCAAGC 
ATTTATGGCA GCTACCGGAA CTTTGTGGGG CCTCCTCACT TCCAGGTCAT CTGCCGGCTG 
CTTGGCTACC AGGGCATTGC AGTCGTCATG GAAGAACTGC TGAAGGTTGT CAAGAGCCTG 
CTTCAAGGCA CAATCCTGCA GTATGTGAAA ACTCTGATGG AAGTGATGCC TAAGATCTGC 
CGCCTTCCCC GACATGAGTA CGGCTCTCCT GGCATCCTAG AGTTCTTCCA CCACCAGCTG 
AAGGACATTG TGGAGTATGC AGAGCTGAAA ACAGTATGCT TCCAGAACCT GCGGGAGGTG 
GGAAATGCTG TTCTCTTCTG CCTGCTCATC GAGCAAAGCC TGTCTTTAGA AGAAGTCTGT 
GACCTGTTGC ATGCAGCTCC TTTTCAGAAT ATCCTGCCTC GAGTCCACGT AAAAGAGGGG 
GAGAGAGTTG ATGCCAAAAT GAAAAGACTA GAATCCAAGT ATGCTCCACT GCATCTTGTT 
CCGCTGATTG AAAGGCTGGG GACCCCACAG CAAATTGCAA TCGCAAGAGA GGGGGACTTG 
CTGACCAAGG AGCGGCTCTG TTGTGGTCTG TCCATGTTTG AAGTCATCCT CACACGGATC 
CGGACCTTTC TGGATGATCC CATCTGGCGT GGGCCCCTGC CCAGCAATGG GGTCATGCAT 
GTGGATGAGT GTGTGGAGTT TCACAGACTA TGGAGTGCCA TGCAGTTTGT TTACTGCATT 
CCTGTAGGGA CACACGAGTT TACAGTGGAG CAGTGTTTTG GCGATGGGCT CCACTGGGCT 
GGCTGCATGA TCATTGTACT TCTGGGACAG CAGCGGCGCT TTGCTGTGTT GGATTTCTGC 
TATCATCTTC TCAAAGTTCA GAAACACGAT GGCAAGGATG AGATCATCAA AAATGTGCCA 
TTGAAGAAGA TGGTGGAGAG GATTCGCAAG TTCCAGATTC TCAATGATGA GATCATCACT 
ATCCTGGACA AGTACTTGAA ATCTGGAGAT GGGGAGAGCA CACCTGTGGA ACATGTACGC 
TGCTTCCAGC CACCCATCCA CCAGTCCCTA GCCAGCAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001100987.1
CDS	91..3852
Translation	MAAQVTLEDALSNVDLLEELPLPDQQPCIEPPPSSLLYQPNFNTNFEDRNAFVTGIARYIEQATVHSSMNEMLEEGQEYAVMLYTWRSCSRAIPQVKCNEQPNRVEIYEKTVEVLEPEVTKLMNFMYFQRNAIERFCGEVRRLCHAERRKDFVSEAYLITLGKFINMFAVLDELKNMKCSVKNDHSAYKRAAQFLRKMADPQSIQESQNLSMFLANHNKITQSLQQQLEVISGYEELLADIVNLCVDYYENRMYLTPSEKHMLLKVMGFGLYLMDGSVSNIYKLDAKKRINLSKIDKYFKQLQVVPLFGDMQIELARYIKTSAHYEENKSRWTCTSSSSSPQYNICEQMIQIREDHMRFISELARYSNSEVVTGSGRQEAQKTDAEYRKLFDLALQGLQLLSQWSAHVMEVYSWKLVHPTDKYSNKDCPDNAEEYERATRYNYTTEEKFALVEVIAMIKGLQVLMGRMESVFNHAIRHTVYAALQDFSQVTLREPLRQAIKKKKNVIQSVLQAIRKTVCDWETGHEPFNDPALRGEKDPKSGFDIKVPRRAVGPSSTQLYMVRTMLESLIADKSGSKKTLRSSLEGPTILDIEKFHRESFFYTHLINFSETLQQCCDLSQLWFREFFLELTMGRRIQFPIEMSMPWILTDHILETKEASMMEYVLYSLDLYNDSAHYALTKFNKQFLYDEIEAEVNLCFDQFVYKLADQIFAYYKVMAGSLLLDKRLRSECKNQGATIHLPPSNRYETLLKQRHVQLLGRSIDLNRLITQRVSAAMYKSLELAIGRFESEDLTSVVELDGLLEINRMTHKLLSRYLTLDSFDAMFREANHNVSAPYGRITLHVFWELNYDFLPNYCYNGSTNRFVRTVLPFSQEFQRDKQPNAQPQYLHGSKALNLAYSSIYGSYRNFVGPPHFQVICRLLGYQGIAVVMEELLKVVKSLLQGTILQYVKTLMEVMPKICRLPRHEYGSPGILEFFHHQLKDIVEYAELKTVCFQNLREVGNAVLFCLLIEQSLSLEEVCDLLHAAPFQNILPRVHVKEGERVDAKMKRLESKYAPLHLVPLIERLGTPQQIAIAREGDLLTKERLCCGLSMFEVILTRIRTFLDDPIWRGPLPSNGVMHVDECVEFHRLWSAMQFVYCIPVGTHEFTVEQCFGDGLHWAGCMIIVLLGQQRRFAVLDFCYHLLKVQKHDGKDEIIKNVPLKKMVERIRKFQILNDEIITILDKYLKSGDGESTPVEHVRCFQPPIHQSLASS

Target ORF information:

RefSeq Version	NM_001107517.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus cytoplasmic FMR1 interacting protein 1 (Cyfip1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001107517.1

ORF Insert Sequence:

ATGGCTGCCC AAGTGACACT AGAGGACGCA CTGTCCAATG TGGACCTCCT GGAAGAGCTG 
CCTCTTCCTG ACCAGCAGCC CTGCATTGAG CCCCCACCTT CCTCGCTGCT CTATCAGCCA 
AATTTCAACA CCAACTTTGA AGACCGAAAT GCATTTGTCA CTGGCATTGC AAGATACATT 
GAACAAGCAA CTGTCCACTC TAGCATGAAC GAGATGCTAG AGGAAGGCCA AGAGTATGCT 
GTCATGCTGT ATACCTGGAG GAGCTGCTCC CGTGCCATCC CTCAGGTGAA GTGCAATGAG 
CAGCCTAATA GGGTTGAAAT TTATGAGAAA ACTGTGGAAG TCCTTGAACC CGAGGTCACA 
AAACTGATGA ATTTTATGTA CTTCCAGAGA AATGCCATCG AGCGTTTCTG TGGGGAGGTG 
AGACGCCTGT GTCATGCTGA GAGGAGAAAG GACTTCGTGT CTGAAGCCTA CTTGATCACC 
TTGGGCAAGT TTATCAACAT GTTTGCTGTG TTGGATGAGC TGAAGAACAT GAAGTGCAGT 
GTGAAGAATG ACCACTCAGC GTATAAGAGG GCTGCTCAGT TTTTACGTAA AATGGCAGAT 
CCACAATCCA TCCAGGAGTC ACAGAATTTG TCCATGTTCC TGGCCAACCA CAACAAGATT 
ACACAATCTC TACAGCAACA GCTTGAGGTC ATTTCTGGCT ATGAAGAACT CCTGGCAGAC 
ATAGTGAATC TGTGTGTGGA CTACTATGAG AACAGAATGT ACTTGACGCC CAGCGAAAAA 
CACATGCTTC TCAAAGTCAT GGGATTTGGT CTGTACCTGA TGGATGGGAG TGTGAGTAAC 
ATCTACAAAC TAGATGCCAA GAAAAGAATA AATTTATCCA AAATAGACAA GTATTTCAAG 
CAGCTGCAGG TGGTTCCACT CTTTGGAGAT ATGCAGATAG AACTGGCAAG ATACATCAAG 
ACCAGCGCAC ACTATGAGGA GAATAAGTCC CGGTGGACCT GTACGTCCTC GAGCAGCAGC 
CCACAGTACA ACATCTGTGA GCAGATGATC CAGATCCGTG AGGACCACAT GCGTTTCATT 
TCTGAGCTGG CACGCTACAG CAATAGCGAG GTAGTCACAG GGTCTGGCCG GCAGGAGGCC 
CAGAAGACAG ATGCTGAGTA CCGGAAGCTC TTTGACCTGG CGCTGCAGGG CCTGCAGCTT 
CTGTCGCAGT GGAGCGCGCA TGTGATGGAA GTGTATTCCT GGAAACTTGT ACATCCAACA 
GACAAGTACT CCAACAAGGA CTGCCCAGAC AATGCTGAGG AGTATGAACG TGCCACGCGT 
TACAACTACA CCACTGAGGA GAAGTTTGCT CTGGTGGAGG TGATTGCCAT GATCAAAGGC 
CTGCAGGTGC TGATGGGCAG GATGGAGAGT GTGTTCAATC ACGCCATCAG ACACACAGTT 
TATGCTGCAC TTCAGGACTT CTCCCAGGTG ACCCTCAGGG AACCACTGCG ACAGGCCATC 
AAGAAGAAAA AGAATGTCAT CCAGAGTGTC CTACAGGCCA TCAGGAAGAC TGTGTGTGAC 
TGGGAGACTG GGCATGAACC CTTTAACGAC CCAGCTCTGC GGGGAGAGAA GGACCCTAAG 
AGCGGCTTTG ACATCAAGGT GCCACGCCGT GCTGTAGGAC CCTCCAGCAC ACAGCTTTAC 
ATGGTGAGAA CTATGCTAGA GTCCCTCATT GCAGACAAAA GTGGTTCCAA GAAAACCTTG 
AGAAGTAGCC TTGAGGGGCC CACCATATTG GACATAGAAA AATTTCATCG AGAATCATTC 
TTCTACACTC ACTTGATAAA TTTCAGTGAA ACACTGCAGC AGTGCTGTGA CCTTTCCCAG 
CTGTGGTTCC GAGAGTTCTT CCTGGAGCTG ACCATGGGCA GGAGGATCCA GTTCCCCATT 
GAGATGTCTA TGCCCTGGAT TCTGACTGAC CACATCCTAG AGACCAAGGA GGCATCAATG 
ATGGAGTACG TCCTCTACTC CTTGGACCTG TACAATGACA GTGCTCACTA TGCGCTCACC 
AAGTTCAACA AGCAGTTTCT CTATGATGAA ATTGAAGCAG AGGTGAATCT GTGTTTTGAC 
CAATTTGTTT ATAAACTGGC AGACCAGATA TTTGCATATT ACAAGGTTAT GGCAGGAAGT 
TTGCTTCTTG ACAAACGGTT ACGATCAGAA TGCAAAAATC AGGGAGCCAC GATTCACCTC 
CCACCGTCTA ACCGCTATGA GACGTTGTTG AAGCAAAGGC ACGTGCAGCT GCTTGGGAGA 
TCCATCGATC TCAATCGTCT GATTACACAG CGTGTTTCAG CAGCCATGTA CAAATCTCTA 
GAACTGGCAA TTGGACGATT TGAAAGTGAA GATTTAACAT CAGTAGTCGA GCTAGATGGG 
CTCTTAGAAA TCAACCGTAT GACGCACAAG CTGCTGAGTA GGTACCTCAC ACTAGACAGC 
TTTGATGCCA TGTTCCGGGA AGCCAACCAC AACGTGTCTG CACCTTATGG AAGAATCACC 
CTCCATGTCT TCTGGGAGCT AAACTATGAC TTCTTGCCCA ACTACTGCTA TAATGGCTCC 
ACCAACCGGT TTGTTCGGAC AGTACTACCA TTTTCTCAGG AATTTCAACG AGACAAACAA 
CCTAATGCAC AGCCCCAGTA TTTGCACGGC TCCAAGGCTT TGAACCTAGC CTACTCAAGC 
ATTTATGGCA GCTACCGGAA CTTTGTGGGG CCTCCTCACT TCCAGGTCAT CTGCCGGCTG 
CTTGGCTACC AGGGCATTGC AGTCGTCATG GAAGAACTGC TGAAGGTTGT CAAGAGCCTG 
CTTCAAGGCA CAATCCTGCA GTATGTGAAA ACTCTGATGG AAGTGATGCC TAAGATCTGC 
CGCCTTCCCC GACATGAGTA CGGCTCTCCT GGCATCCTAG AGTTCTTCCA CCACCAGCTG 
AAGGACATTG TGGAGTATGC AGAGCTGAAA ACAGTATGCT TCCAGAACCT GCGGGAGGTG 
GGAAATGCTG TTCTCTTCTG CCTGCTCATC GAGCAAAGCC TGTCTTTAGA AGAAGTCTGT 
GACCTGTTGC ATGCAGCTCC TTTTCAGAAT ATCCTGCCTC GAGTCCACGT AAAAGAGGGG 
GAGAGAGTTG ATGCCAAAAT GAAAAGACTA GAATCCAAGT ATGCTCCACT GCATCTTGTT 
CCGCTGATTG AAAGGCTGGG GACCCCACAG CAAATTGCAA TCGCAAGAGA GGGGGACTTG 
CTGACCAAGG AGCGGCTCTG TTGTGGTCTG TCCATGTTTG AAGTCATCCT CACACGGATC 
CGGACCTTTC TGGATGATCC CATCTGGCGT GGGCCCCTGC CCAGCAATGG GGTCATGCAT 
GTGGATGAGT GTGTGGAGTT TCACAGACTA TGGAGTGCCA TGCAGTTTGT TTACTGCATT 
CCTGTAGGGA CACACGAGTT TACAGTGGAG CAGTGTTTTG GCGATGGGCT CCACTGGGCT 
GGCTGCATGA TCATTGTACT TCTGGGACAG CAGCGGCGCT TTGCTGTGTT GGATTTCTGC 
TATCATCTTC TCAAAGTTCA GAAACACGAT GGCAAGGATG AGATCATCAA AAATGTGCCA 
TTGAAGAAGA TGGTGGAGAG GATTCGCAAG TTCCAGATTC TCAATGATGA GATCATCACT 
ATCCTGGACA AGTACTTGAA ATCTGGAGAT GGGGAGAGCA CACCTGTGGA ACATGTACGC 
TGCTTCCAGC CACCCATCCA CCAGTCCCTA GCCAGCAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cyfip1 ( XM_006229288.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006229350.1 Rattus norvegicus cytoplasmic FMR1 interacting protein 1 (Cyfip1), transcript variant X1, mRNA.

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CloneID	ORa22853
Clone ID Related Accession (Same CDS sequence)	XM_006229288.2
Accession Version	XM_006229288.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3756bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	cytoplasmic FMR1-interacting protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Aug 7, 2014 this sequence version replaced XM_006229288.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCCC AAGTGACACT AGAGGACGCA CTGTCCAATG TGGACCTCCT GGAAGAGCTG 
CCTCTTCCTG ACCAGCAGCC CTGCATTGAG CCCCCACCTT CCTCGCTGCT CTATCAGCCA 
AATTTCAACA CCAACTTTGA AGACCGAAAT GCATTTGTCA CTGGCATTGC AAGATACATT 
GAACAAGCAA CTGTCCACTC TAGCATGAAC GAGATGCTAG AGGAAGGCCA AGAGTATGCT 
GTCATGCTGT ATACCTGGAG GAGCTGCTCC CGTGCCATCC CTCAGGTGAA GTGCAATGAG 
CAGCCTAATA GGGTTGAAAT TTATGAGAAA ACTGTGGAAG TCCTTGAACC CGAGGTCACA 
AAACTGATGA ATTTTATGTA CTTCCAGAGA AATGCCATCG AGCGTTTCTG TGGGGAGGTG 
AGACGCCTGT GTCATGCTGA GAGGAGAAAG GACTTCGTGT CTGAAGCCTA CTTGATCACC 
TTGGGCAAGT TTATCAACAT GTTTGCTGTG TTGGATGAGC TGAAGAACAT GAAGTGCAGT 
GTGAAGAATG ACCACTCAGC GTATAAGAGG GCTGCTCAGT TTTTACGTAA AATGGCAGAT 
CCACAATCCA TCCAGGAGTC ACAGAATTTG TCCATGTTCC TGGCCAACCA CAACAAGATT 
ACACAATCTC TACAGCAACA GCTTGAGGTC ATTTCTGGCT ATGAAGAACT CCTGGCAGAC 
ATAGTGAATC TGTGTGTGGA CTACTATGAG AACAGAATGT ACTTGACGCC CAGCGAAAAA 
CACATGCTTC TCAAAGTCAT GGGATTTGGT CTGTACCTGA TGGATGGGAG TGTGAGTAAC 
ATCTACAAAC TAGATGCCAA GAAAAGAATA AATTTATCCA AAATAGACAA GTATTTCAAG 
CAGCTGCAGG TGGTTCCACT CTTTGGAGAT ATGCAGATAG AACTGGCAAG ATACATCAAG 
ACCAGCGCAC ACTATGAGGA GAATAAGTCC CGGTGGACCT GTACGTCCTC GAGCAGCAGC 
CCACAGTACA ACATCTGTGA GCAGATGATC CAGATCCGTG AGGACCACAT GCGTTTCATT 
TCTGAGCTGG CACGCTACAG CAATAGCGAG GTAGTCACAG GGTCTGGCCG GCAGGAGGCC 
CAGAAGACAG ATGCTGAGTA CCGGAAGCTC TTTGACCTGG CGCTGCAGGG CCTGCAGCTT 
CTGTCGCAGT GGAGCGCGCA TGTGATGGAA GTGTATTCCT GGAAACTTGT ACATCCAACA 
GACAAGTACT CCAACAAGGA CTGCCCAGAC AATGCTGAGG AGTATGAACG TGCCACGCGT 
TACAACTACA CCACTGAGGA GAAGTTTGCT CTGGTGGAGG TGATTGCCAT GATCAAAGGC 
CTGCAGGTGC TGATGGGCAG GATGGAGAGT GTGTTCAATC ACGCCATCAG ACACACAGTT 
TATGCTGCAC TTCAGGACTT CTCCCAGGTG ACCCTCAGGG AACCACTGCG ACAGGCCATC 
AAGAAGAAAA AGAATGTCAT CCAGAGTGTC CTACAGGCCA TCAGGAAGAC TGTGTGTGAC 
TGGGAGACTG GGCATGAACC CTTTAACGAC CCAGCTCTGC GGGGAGAGAA GGACCCTAAG 
AGCGGCTTTG ACATCAAGGT GCCACGCCGT GCTGTAGGAC CCTCCAGCAC ACAGCTCTAC 
CTGGTGCGCA CTATGGCTGA GTCCTTGAGC TCTGCCGAGC TGCTGAGGCA GCTCAAGTCT 
CTGGGCATGG AAAGGCTCTT GCATGTGGTA AACGCGTTTC TGAGGCAGTC GTACACCTAT 
CCACCTCTTT TAACCTTTGG TGAAACACTG CAGCAGTGCT GTGACCTTTC CCAGCTGTGG 
TTCCGAGAGT TCTTCCTGGA GCTGACCATG GGCAGGAGGA TCCAGTTCCC CATTGAGATG 
TCTATGCCCT GGATTCTGAC TGACCACATC CTAGAGACCA AGGAGGCATC AATGATGGAG 
TACGTCCTCT ACTCCTTGGA CCTGTACAAT GACAGTGCTC ACTATGCGCT CACCAAGTTC 
AACAAGCAGT TTCTCTATGA TGAAATTGAA GCAGAGGTGA ATCTGTGTTT TGACCAATTT 
GTTTATAAAC TGGCAGACCA GATATTTGCA TATTACAAGG TTATGGCAGG AAGTTTGCTT 
CTTGACAAAC GGTTACGATC AGAATGCAAA AATCAGGGAG CCACGATTCA CCTCCCACCG 
TCTAACCGCT ATGAGACGTT GTTGAAGCAA AGGCACGTGC AGCTGCTTGG GAGATCCATC 
GATCTCAATC GTCTGATTAC ACAGCGTGTT TCAGCAGCCA TGTACAAATC TCTAGAACTG 
GCAATTGGAC GATTTGAAAG TGAAGATTTA ACATCAGTAG TCGAGCTAGA TGGGCTCTTA 
GAAATCAACC GTATGACGCA CAAGCTGCTG AGTAGGTACC TCACACTAGA CAGCTTTGAT 
GCCATGTTCC GGGAAGCCAA CCACAACGTG TCTGCACCTT ATGGAAGAAT CACCCTCCAT 
GTCTTCTGGG AGCTAAACTA TGACTTCTTG CCCAACTACT GCTATAATGG CTCCACCAAC 
CGGTTTGTTC GGACAGTACT ACCATTTTCT CAGGAATTTC AACGAGACAA ACAACCTAAT 
GCACAGCCCC AGTATTTGCA CGGCTCCAAG GCTTTGAACC TAGCCTACTC AAGCATTTAT 
GGCAGCTACC GGAACTTTGT GGGGCCTCCT CACTTCCAGG TCATCTGCCG GCTGCTTGGC 
TACCAGGGCA TTGCAGTCGT CATGGAAGAA CTGCTGAAGG TTGTCAAGAG CCTGCTTCAA 
GGCACAATCC TGCAGTATGT GAAAACTCTG ATGGAAGTGA TGCCTAAGAT CTGCCGCCTT 
CCCCGACATG AGTACGGCTC TCCTGGCATC CTAGAGTTCT TCCACCACCA GCTGAAGGAC 
ATTGTGGAGT ATGCAGAGCT GAAAACAGTA TGCTTCCAGA ACCTGCGGGA GGTGGGAAAT 
GCTGTTCTCT TCTGCCTGCT CATCGAGCAA AGCCTGTCTT TAGAAGAAGT CTGTGACCTG 
TTGCATGCAG CTCCTTTTCA GAATATCCTG CCTCGAGTCC ACGTAAAAGA GGGGGAGAGA 
GTTGATGCCA AAATGAAAAG ACTAGAATCC AAGTATGCTC CACTGCATCT TGTTCCGCTG 
ATTGAAAGGC TGGGGACCCC ACAGCAAATT GCAATCGCAA GAGAGGGGGA CTTGCTGACC 
AAGGAGCGGC TCTGTTGTGG TCTGTCCATG TTTGAAGTCA TCCTCACACG GATCCGGACC 
TTTCTGGATG ATCCCATCTG GCGTGGGCCC CTGCCCAGCA ATGGGGTCAT GCATGTGGAT 
GAGTGTGTGG AGTTTCACAG ACTATGGAGT GCCATGCAGT TTGTTTACTG CATTCCTGTA 
GGGACACACG AGTTTACAGT GGAGCAGTGT TTTGGCGATG GGCTCCACTG GGCTGGCTGC 
ATGATCATTG TACTTCTGGG ACAGCAGCGG CGCTTTGCTG TGTTGGATTT CTGCTATCAT 
CTTCTCAAAG TTCAGAAACA CGATGGCAAG GATGAGATCA TCAAAAATGT GCCATTGAAG 
AAGATGGTGG AGAGGATTCG CAAGTTCCAG ATTCTCAATG ATGAGATCAT CACTATCCTG 
GACAAGTACT TGAAATCTGG AGATGGGGAG AGCACACCTG TGGAACATGT ACGCTGCTTC 
CAGCCACCCA TCCACCAGTC CCTAGCCAGC AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006229350.1
CDS	111..3866
Translation	MAAQVTLEDALSNVDLLEELPLPDQQPCIEPPPSSLLYQPNFNTNFEDRNAFVTGIARYIEQATVHSSMNEMLEEGQEYAVMLYTWRSCSRAIPQVKCNEQPNRVEIYEKTVEVLEPEVTKLMNFMYFQRNAIERFCGEVRRLCHAERRKDFVSEAYLITLGKFINMFAVLDELKNMKCSVKNDHSAYKRAAQFLRKMADPQSIQESQNLSMFLANHNKITQSLQQQLEVISGYEELLADIVNLCVDYYENRMYLTPSEKHMLLKVMGFGLYLMDGSVSNIYKLDAKKRINLSKIDKYFKQLQVVPLFGDMQIELARYIKTSAHYEENKSRWTCTSSSSSPQYNICEQMIQIREDHMRFISELARYSNSEVVTGSGRQEAQKTDAEYRKLFDLALQGLQLLSQWSAHVMEVYSWKLVHPTDKYSNKDCPDNAEEYERATRYNYTTEEKFALVEVIAMIKGLQVLMGRMESVFNHAIRHTVYAALQDFSQVTLREPLRQAIKKKKNVIQSVLQAIRKTVCDWETGHEPFNDPALRGEKDPKSGFDIKVPRRAVGPSSTQLYLVRTMAESLSSAELLRQLKSLGMERLLHVVNAFLRQSYTYPPLLTFGETLQQCCDLSQLWFREFFLELTMGRRIQFPIEMSMPWILTDHILETKEASMMEYVLYSLDLYNDSAHYALTKFNKQFLYDEIEAEVNLCFDQFVYKLADQIFAYYKVMAGSLLLDKRLRSECKNQGATIHLPPSNRYETLLKQRHVQLLGRSIDLNRLITQRVSAAMYKSLELAIGRFESEDLTSVVELDGLLEINRMTHKLLSRYLTLDSFDAMFREANHNVSAPYGRITLHVFWELNYDFLPNYCYNGSTNRFVRTVLPFSQEFQRDKQPNAQPQYLHGSKALNLAYSSIYGSYRNFVGPPHFQVICRLLGYQGIAVVMEELLKVVKSLLQGTILQYVKTLMEVMPKICRLPRHEYGSPGILEFFHHQLKDIVEYAELKTVCFQNLREVGNAVLFCLLIEQSLSLEEVCDLLHAAPFQNILPRVHVKEGERVDAKMKRLESKYAPLHLVPLIERLGTPQQIAIAREGDLLTKERLCCGLSMFEVILTRIRTFLDDPIWRGPLPSNGVMHVDECVEFHRLWSAMQFVYCIPVGTHEFTVEQCFGDGLHWAGCMIIVLLGQQRRFAVLDFCYHLLKVQKHDGKDEIIKNVPLKKMVERIRKFQILNDEIITILDKYLKSGDGESTPVEHVRCFQPPIHQSLASS

Target ORF information:

RefSeq Version	XM_006229288.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus cytoplasmic FMR1 interacting protein 1 (Cyfip1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006229288.2

ORF Insert Sequence:

ATGGCTGCCC AAGTGACACT AGAGGACGCA CTGTCCAATG TGGACCTCCT GGAAGAGCTG 
CCTCTTCCTG ACCAGCAGCC CTGCATTGAG CCCCCACCTT CCTCGCTGCT CTATCAGCCA 
AATTTCAACA CCAACTTTGA AGACCGAAAT GCATTTGTCA CTGGCATTGC AAGATACATT 
GAACAAGCAA CTGTCCACTC TAGCATGAAC GAGATGCTAG AGGAAGGCCA AGAGTATGCT 
GTCATGCTGT ATACCTGGAG GAGCTGCTCC CGTGCCATCC CTCAGGTGAA GTGCAATGAG 
CAGCCTAATA GGGTTGAAAT TTATGAGAAA ACTGTGGAAG TCCTTGAACC CGAGGTCACA 
AAACTGATGA ATTTTATGTA CTTCCAGAGA AATGCCATCG AGCGTTTCTG TGGGGAGGTG 
AGACGCCTGT GTCATGCTGA GAGGAGAAAG GACTTCGTGT CTGAAGCCTA CTTGATCACC 
TTGGGCAAGT TTATCAACAT GTTTGCTGTG TTGGATGAGC TGAAGAACAT GAAGTGCAGT 
GTGAAGAATG ACCACTCAGC GTATAAGAGG GCTGCTCAGT TTTTACGTAA AATGGCAGAT 
CCACAATCCA TCCAGGAGTC ACAGAATTTG TCCATGTTCC TGGCCAACCA CAACAAGATT 
ACACAATCTC TACAGCAACA GCTTGAGGTC ATTTCTGGCT ATGAAGAACT CCTGGCAGAC 
ATAGTGAATC TGTGTGTGGA CTACTATGAG AACAGAATGT ACTTGACGCC CAGCGAAAAA 
CACATGCTTC TCAAAGTCAT GGGATTTGGT CTGTACCTGA TGGATGGGAG TGTGAGTAAC 
ATCTACAAAC TAGATGCCAA GAAAAGAATA AATTTATCCA AAATAGACAA GTATTTCAAG 
CAGCTGCAGG TGGTTCCACT CTTTGGAGAT ATGCAGATAG AACTGGCAAG ATACATCAAG 
ACCAGCGCAC ACTATGAGGA GAATAAGTCC CGGTGGACCT GTACGTCCTC GAGCAGCAGC 
CCACAGTACA ACATCTGTGA GCAGATGATC CAGATCCGTG AGGACCACAT GCGTTTCATT 
TCTGAGCTGG CACGCTACAG CAATAGCGAG GTAGTCACAG GGTCTGGCCG GCAGGAGGCC 
CAGAAGACAG ATGCTGAGTA CCGGAAGCTC TTTGACCTGG CGCTGCAGGG CCTGCAGCTT 
CTGTCGCAGT GGAGCGCGCA TGTGATGGAA GTGTATTCCT GGAAACTTGT ACATCCAACA 
GACAAGTACT CCAACAAGGA CTGCCCAGAC AATGCTGAGG AGTATGAACG TGCCACGCGT 
TACAACTACA CCACTGAGGA GAAGTTTGCT CTGGTGGAGG TGATTGCCAT GATCAAAGGC 
CTGCAGGTGC TGATGGGCAG GATGGAGAGT GTGTTCAATC ACGCCATCAG ACACACAGTT 
TATGCTGCAC TTCAGGACTT CTCCCAGGTG ACCCTCAGGG AACCACTGCG ACAGGCCATC 
AAGAAGAAAA AGAATGTCAT CCAGAGTGTC CTACAGGCCA TCAGGAAGAC TGTGTGTGAC 
TGGGAGACTG GGCATGAACC CTTTAACGAC CCAGCTCTGC GGGGAGAGAA GGACCCTAAG 
AGCGGCTTTG ACATCAAGGT GCCACGCCGT GCTGTAGGAC CCTCCAGCAC ACAGCTCTAC 
CTGGTGCGCA CTATGGCTGA GTCCTTGAGC TCTGCCGAGC TGCTGAGGCA GCTCAAGTCT 
CTGGGCATGG AAAGGCTCTT GCATGTGGTA AACGCGTTTC TGAGGCAGTC GTACACCTAT 
CCACCTCTTT TAACCTTTGG TGAAACACTG CAGCAGTGCT GTGACCTTTC CCAGCTGTGG 
TTCCGAGAGT TCTTCCTGGA GCTGACCATG GGCAGGAGGA TCCAGTTCCC CATTGAGATG 
TCTATGCCCT GGATTCTGAC TGACCACATC CTAGAGACCA AGGAGGCATC AATGATGGAG 
TACGTCCTCT ACTCCTTGGA CCTGTACAAT GACAGTGCTC ACTATGCGCT CACCAAGTTC 
AACAAGCAGT TTCTCTATGA TGAAATTGAA GCAGAGGTGA ATCTGTGTTT TGACCAATTT 
GTTTATAAAC TGGCAGACCA GATATTTGCA TATTACAAGG TTATGGCAGG AAGTTTGCTT 
CTTGACAAAC GGTTACGATC AGAATGCAAA AATCAGGGAG CCACGATTCA CCTCCCACCG 
TCTAACCGCT ATGAGACGTT GTTGAAGCAA AGGCACGTGC AGCTGCTTGG GAGATCCATC 
GATCTCAATC GTCTGATTAC ACAGCGTGTT TCAGCAGCCA TGTACAAATC TCTAGAACTG 
GCAATTGGAC GATTTGAAAG TGAAGATTTA ACATCAGTAG TCGAGCTAGA TGGGCTCTTA 
GAAATCAACC GTATGACGCA CAAGCTGCTG AGTAGGTACC TCACACTAGA CAGCTTTGAT 
GCCATGTTCC GGGAAGCCAA CCACAACGTG TCTGCACCTT ATGGAAGAAT CACCCTCCAT 
GTCTTCTGGG AGCTAAACTA TGACTTCTTG CCCAACTACT GCTATAATGG CTCCACCAAC 
CGGTTTGTTC GGACAGTACT ACCATTTTCT CAGGAATTTC AACGAGACAA ACAACCTAAT 
GCACAGCCCC AGTATTTGCA CGGCTCCAAG GCTTTGAACC TAGCCTACTC AAGCATTTAT 
GGCAGCTACC GGAACTTTGT GGGGCCTCCT CACTTCCAGG TCATCTGCCG GCTGCTTGGC 
TACCAGGGCA TTGCAGTCGT CATGGAAGAA CTGCTGAAGG TTGTCAAGAG CCTGCTTCAA 
GGCACAATCC TGCAGTATGT GAAAACTCTG ATGGAAGTGA TGCCTAAGAT CTGCCGCCTT 
CCCCGACATG AGTACGGCTC TCCTGGCATC CTAGAGTTCT TCCACCACCA GCTGAAGGAC 
ATTGTGGAGT ATGCAGAGCT GAAAACAGTA TGCTTCCAGA ACCTGCGGGA GGTGGGAAAT 
GCTGTTCTCT TCTGCCTGCT CATCGAGCAA AGCCTGTCTT TAGAAGAAGT CTGTGACCTG 
TTGCATGCAG CTCCTTTTCA GAATATCCTG CCTCGAGTCC ACGTAAAAGA GGGGGAGAGA 
GTTGATGCCA AAATGAAAAG ACTAGAATCC AAGTATGCTC CACTGCATCT TGTTCCGCTG 
ATTGAAAGGC TGGGGACCCC ACAGCAAATT GCAATCGCAA GAGAGGGGGA CTTGCTGACC 
AAGGAGCGGC TCTGTTGTGG TCTGTCCATG TTTGAAGTCA TCCTCACACG GATCCGGACC 
TTTCTGGATG ATCCCATCTG GCGTGGGCCC CTGCCCAGCA ATGGGGTCAT GCATGTGGAT 
GAGTGTGTGG AGTTTCACAG ACTATGGAGT GCCATGCAGT TTGTTTACTG CATTCCTGTA 
GGGACACACG AGTTTACAGT GGAGCAGTGT TTTGGCGATG GGCTCCACTG GGCTGGCTGC 
ATGATCATTG TACTTCTGGG ACAGCAGCGG CGCTTTGCTG TGTTGGATTT CTGCTATCAT 
CTTCTCAAAG TTCAGAAACA CGATGGCAAG GATGAGATCA TCAAAAATGT GCCATTGAAG 
AAGATGGTGG AGAGGATTCG CAAGTTCCAG ATTCTCAATG ATGAGATCAT CACTATCCTG 
GACAAGTACT TGAAATCTGG AGATGGGGAG AGCACACCTG TGGAACATGT ACGCTGCTTC 
CAGCCACCCA TCCACCAGTC CCTAGCCAGC AGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Retrolinkin recruits the WAVE1 protein complex to facilitate BDNF-induced TrkB endocytosis and dendrite outgrowth.
Molecular biology of the cell27(21)3342-3356(2016 Nov)
Xu C,Fu X,Zhu S,Liu JJ

Cyfip1 Regulates Presynaptic Activity during Development.
The Journal of neuroscience : the official journal of the Society for Neuroscience36(5)1564-76(2016 Feb)
Hsiao K,Harony-Nicolas H,Buxbaum JD,Bozdagi-Gunal O,Benson DL

Up-regulated cytoplasmic FMRP-interacting protein 1 in intractable temporal lobe epilepsy patients and a rat model.
The International journal of neuroscience1-10(2015 Aug)
Huang Y

Two-stage translational control of dentate gyrus LTP consolidation is mediated by sustained BDNF-TrkB signaling to MNK.
Cell reports9(4)1430-45(2014 Nov)
Panja D,Kenney JW,D'Andrea L,Zalfa F,Vedeler A,Wibrand K,Fukunaga R,Bagni C,Proud CG,Bramham CR

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Cyfip1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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