Nhsl1 gene cDNA ORF clone, Rattus norvegicus(Norway rat)

mRNA	Protein	Name
XM_002728625.5	XP_002728671.1	NHS-like protein 1 isoform X5
XM_006227671.3	XP_006227733.1	NHS-like protein 1 isoform X7
XM_017587917.1	XP_017443406.1	NHS-like protein 1 isoform X1
XM_006222831.3	XP_006222893.1	NHS-like protein 1 isoform X2
XM_017587918.1	XP_017443407.1	NHS-like protein 1 isoform X3
XM_006222833.3	XP_006222895.1	NHS-like protein 1 isoform X6
XM_017589824.1	XP_017445313.1	NHS-like protein 1 isoform X3
XM_002728626.5	XP_002728672.1	NHS-like protein 1 isoform X4
XM_017589823.1	XP_017445312.1	NHS-like protein 1 isoform X1
XM_002725450.5	XP_002725496.1	NHS-like protein 1 isoform X4
XM_006222834.3	XP_006222896.1	NHS-like protein 1 isoform X7
XM_006227668.3	XP_006227730.1	NHS-like protein 1 isoform X2
XM_002725449.5	XP_002725495.1	NHS-like protein 1 isoform X5
XM_006227670.3	XP_006227732.1	NHS-like protein 1 isoform X6

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa13975	XM_006222831.3 Latest version!	Rattus norvegicus NHS-like 1 (Nhsl1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
ORa13975	XM_006227668.3 Latest version!	Rattus norvegicus NHS-like 1 (Nhsl1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
ORa13977	XM_002728626.5 Latest version!	Rattus norvegicus NHS-like 1 (Nhsl1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
ORa13977	XM_002725450.5 Latest version!	Rattus norvegicus NHS-like 1 (Nhsl1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
ORa13978	XM_002728625.5 Latest version!	Rattus norvegicus NHS-like 1 (Nhsl1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
ORa13978	XM_002725449.5 Latest version!	Rattus norvegicus NHS-like 1 (Nhsl1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
ORa13979	XM_006227670.3 Latest version!	Rattus norvegicus NHS-like 1 (Nhsl1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
ORa13979	XM_006222833.3 Latest version!	Rattus norvegicus NHS-like 1 (Nhsl1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
ORa13980	XM_006227671.3 Latest version!	Rattus norvegicus NHS-like 1 (Nhsl1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
ORa13980	XM_006222834.3 Latest version!	Rattus norvegicus NHS-like 1 (Nhsl1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
ORa53614	XM_017587917.1 Latest version!	Rattus norvegicus NHS-like 1 (Nhsl1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
ORa53614	XM_017589823.1 Latest version!	Rattus norvegicus NHS-like 1 (Nhsl1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
ORa53615	XM_017587918.1 Latest version!	Rattus norvegicus NHS-like 1 (Nhsl1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$811.30 $1159.00
ORa53615	XM_017589824.1 Latest version!	Rattus norvegicus NHS-like 1 (Nhsl1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$811.30 $1159.00

Nhsl1 ( XM_006222831.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006222893.1 Rattus norvegicus NHS-like 1 (Nhsl1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa13975
Clone ID Related Accession (Same CDS sequence)	XM_006222831.3 , XM_006227668.3
Accession Version	XM_006222831.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4914bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	NHS-like protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (AC_000069.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006222831.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAAAGG AAGGCTCTGC AGGTTCCTTC AGAATCAAGG CAAACCCAGG CTCCCTCTCA 
CGTGCCGTGA GTTGGATAAA TTTCTCCTCC CTGTCCAGGC AGACAAAGAG GCTGTTTCGC 
AGCGATGGAG AGCTGTCCGT TTGTGGGCAG CAGGTGGAGG CTGATGATGA GAATTGGATA 
TACAGGACCC AGCCCAGAAA AGCGGTTTCC AACTTAGACG AGGAGAGCCG ATGGACCGTC 
CACTATACGG CGCCGTGGCA CCAGCAGGAG AATGTGTTCC TCCCCTCCAC AAGACCCCCC 
TGTGTTGAGG ACCTGCACCG CCAAGCGAAG CTCAACCTCA AATCAGTGCT GAGGGAATGT 
GACAAGCTGA GGCGCGATGG CTGCCGAAGT TCTCAGTACT ATTCTCAGGG ACCCACCTTT 
GCAGCCAGCT CCAGCCCAGG CGATGACTAT CAAGAGGAAG ATGCAGAAAC AGACCGAAAG 
TATTCTCTCT CTTCATCTGA AGAAGAAAGA TTTATTGGTA TCAGGAGACC TAAAACGCCA 
ACCTCAGGTG ACTTCTCTGA CCTTCATACT CAGACAAACT GGACCAAGTC CCTGCCGCTG 
CCAACACCAG AAGAGAAGAC ACGACAGCAA GCCCAGACAG TCCAGGCTGA TGTGGTTCCT 
ATTAACATAA CTGGGGAGAA TTTTGATCGT CAGGCCAGCC TTCGGCGGTC TCTAATTTAC 
ACTGACACTC TGGTAAGACG ACCGAAGAAA GTCAAAAGGA GAAAGACTAT TTCAGGAATC 
CCTGACATCA TTCAGAAGGA GCTAGCGTCA GCCACGGGCC AAGAGGATGA TGGTAGCACC 
CATTCTCTAT ACTCCCCAGA CCACTACTCT ACATTAGGAA GGCTTGATAG CTATCGGTCC 
ACGGGGCAAT GCTTGGAAAC CAGGGACTCT GGCTGTCAGA CAGAAGAAGT GAAAGTCATC 
CCACCGTCAG TGAGAAGGAT CAGGGCTCAC AAGGGGCTGG GCATTGCTGC CCAGATGAGC 
CACCTCTCGG GTTCCTCTGG CAACATGTCT GTGCTGAGCG ACTCGGCTGG TATCGTGTTC 
CCATCTCGCC TCAATAACGA TACTGGTTTC CACAGCCTTC CACGCGCTGG CGCAAGGGCA 
AGCACGTATT CCCTGGAGGC AAGGATGGGT GCCCTGGGCT CTGCTGAAGA TACGGATGAT 
ACTTTTCCCT ACCGAGGGGG TAGCTCCCAG GGGCCTGAAA ACTTTGCACA TTTAGGGGGT 
GCCTCGAGTA CCGGGATGCT TTCGAGGCCC AAATCCCAGC AGCTGAGGCT CTTGGAGAGC 
CCAGCGTGTG TGGTTTCACC GCACGCGGCC TACTCTACCA GCGTCATCCC AAACGCCACA 
CTGCTGTCCT CTTCGGAGGT TATTGTCATC CACACCGCTC AGAGTGCAGG ACAGCTGGAC 
AGTAGAGTCC CCGGCTCATC CTCATACTCA AAGATAAAAC CCAGAGACCA TCCCACACCC 
AGGTGCTCTG CGAGAGATGA GCGCCAGTCC CCACGACATC ACTGGAACGA GGGTCATATC 
ATTCACTCAC AGGCTTTAGC CTCCGCTGCC CCCGGTGCCA CCGCACTGTT ATCCCTCCGT 
GACTCAGAGG TCTCTCTCAA TGCCCCAGCT ATCAATAGGG AGAACGGGTC CCAAGCCATA 
CTTTATCACT GTAGAAGCAA CCTGGCCTTT CCTGCCCACC CTCCGGATGT GGATGGCAAG 
AGCGAGTGCA GTTCTTCAGG GGACAGGGGA TGTGGCAGTT CTGAGTCCTG GGAATACAAA 
GCCTCCAGCA ATGGGCGGGC TTCCCCACTG AAGCCGCACC TTGCGACTCC CGGTTGCTCT 
ACCCCCACAA GTAACATGAG CAGCTGCAGC TTGGACCAAG CATCCCTCAA GGAGGATGCC 
AGGTCCCTGT ATTCAGAGGA CCACGATGGT TACTATATGA CCGCCCAGGG TGATTCTAGA 
CACGAGGCTG GGAGCGCGTA CGGTATCAGT GATGGCTTTG GAAACCCCAG GCACAGCACG 
GTCAATGTTT TTGACGGAAG GGCTCAGAGA AGCCAAGGGG ATCAGGCCAC TCAGCAGGAT 
AGAATCCTCT CAAGAAACAT CTCTCTGAAG AAAGCAAAAA AACCACCACC GCCACCATCC 
CGGACAGATT CTCTGCGAAG GATTCCCAAG AAGAACAACC AGACAAATGG GCAGGTGCTC 
AACGAGAGCC TGATCGCCTC ACTCCAGCAT TCTCTGCAGC TAAGCCTCCC TGGCCAAGGC 
AGCGGTTCCC CTTCCCAGAG CCCCTGCAGT GACTGTGAGG AACCCTGGCT CCCCAGGTCC 
AGAAGCCAGA GCATTGTTAG CGAGGGTAGT AGCTTGACCT CCACCACCAC TCCCAACGTG 
TATTCTCTGT GCGGGCTCAC TCCATCTCAG AGCGACACAA GCAGCGTCAA GTCGGAATAC 
GCAGACCCCT GGGGCTACTA CATTGACTAC ACAACTTTGC AGGGAGATCC AGGGAACCCC 
ACAGGGGGCT GCCCAGCCAG TAGTGAGGCA GCCACTGGAA ATGGGCCAGT GCGCCACATC 
CAGGAGGGGT CGAGAGCCCC AGTGCCCCAA GTGCCGGGCT GCTCCGTGAA ACCAAAGATC 
ACCTCACCGG AGAAGTCACA GAGAGTCACC TCTCCATCCA GTGGGTATTC TAGCCAGTCG 
AATACACCCA CAGCACTCAC CCCCGTGCCT GTGTTTTTAA AATCGATGTC ACCAGCAAAT 
GGGAAGGGGA AGGCCAAGCC CAAGGTACCA GAAAGAAAAT CGTCTCTGAT CTCTTCAGTG 
TCCATCTCCT CATCGTCCAC TTCCCTCTCC TCTAATACCT CTACGGAAGG AAGTGGGACC 
GTGAAGAAAC TGGATTCAGC CCTGGCCTCG GCCCTTGCTC CTCCCCCTCC TCCTCTTCCC 
GCGCTGCCTT CTCCGAGTCT GGCAAACAAG TCCCCCTTCC TTCCACCTCC TCCTCCTCTA 
GCAGATTGCT CCGAGGGCTC TCCCTTACCG CCCTCTCCTG TGTTCCCCCC TCCACCACCA 
GAAGCCCTTG TTCCCTTCTG CTCCCCCACT GACAGGTGCC TTTCTCCTTC CCCCACAGCT 
TTTAGCCCCC CTCTTCCAAG ATGCTCACCT CCCTTGCCAG CACCTCCACC TTTCTTGCCC 
CCATCAGAAC CCCCACCTGC TCCGCCTCTG GACCCCAAAT TCATGAAAGA AAACAGGCCC 
TTTATCAAAA ACTCTAGCCA GTCCGAATCC TCTAGGGAGG CCCTCAGGCG GCCTGCCAAC 
AAGGAGGAGG GCTGCCGACC CTCGATGCCC CTGATCACCA CAGAAGCTTT GCAGATGGTG 
CAGTTGAGGC CAGTGAGGAA GAACTCAGGT GCTGAGACGG TCCTGTTTTC TGAACCATCA 
GCTCAGGAAC CACGAACCCC GACTGCTCCA CAGTATCACT TAAAGCCATC TGCTTTCCTC 
AAATCCCGAA ATAGCATAAA TGAAATGGAG AGTGAAAGCC AGGCTGCCTC TGTGACGAGC 
TCGCTTCCGA TGCCTGCCAA GAGCCAGAGT CAGGGTGACC ATGACAGTGC AGTCGAGCGT 
GGCGGCCTTC AGAGCTGCAG CGATGGAGCT CCAGGCCCGG GTCCCAGCCT CAGGACCGCT 
CTGCTCCCAG ACTCTTCACC CAGCAGGAAG CCACCCCCCA TCTCCAAGAA GCCCAAACTG 
TTCCTGGTGG TACCACCTCC GCAGAGAGAT TTCACAGCGG AGCCCACAGA GAACGGGAGC 
GAAGCTTTCT CAGGCGTGCC CAGCCCTACC GGGGCAGAGG GGGAAGCTGG GCAGAGCCAA 
GAGGAGAAAT CCAGCCCAGC ATCCCAAGCT GGCTCTCATG CTACGGCCCC CACCTCCGGC 
AGTACGGTTC TAGAAAGAGG AACTGCAGGG TCCTTGTCCC CTGGCGTTGT GGAAGCTAAT 
GCCCCCATGG TCCAACCTAG TACATCACCG GGCCCCGCAC AGGAAGAGGC AGGAGAGAAC 
CGCGTGGATG GTGAGAGAAA TGTGAAGAGC TGCGCGTCTC CACGGGGCGG AGAAGCTGGG 
TTGCCGGAAC CCAACACAGC TGGTCCGTCT TCAGACACGG CAGACGTTTC TAAAGAAGGA 
GGGAACGATG AGGTGCTGAC CCCTACCAGA CCCAGGACAA CGGAAGACCT GTTTGCAGCC 
ATTCACAGAT CCAAAAGGAA AGTCCTTGGT CGGAAGGACT CCGAAGATGA TCACACTCGA 
AATCATTCTC CCTCCCCGCC TGTGACACCC ACAGGTGCTG CCCCTAGCCT GGCCTCTCCA 
AAGCAAGTAG GGTCCATCCA GCGAAGCCTA AAAAAGAGCA GTACCAGCAG CGACAACTTC 
AAAGCTCTGC TGCTCAAGAA GGGGAGTCGC TCGGACACCA GCGCTCGCAT GTCCGCGGCG 
GAGATGCTCA AGTCCACGGA CCCTCGATTC CAGAGATCTA GGTCGGAGCC TTCGCCAGAG 
GTCCCGGACA GCCCATCCAG CTTCTCTCCC AATAAGAACA GGAGAGCTCC GGAGGAGTGG 
GCCAAGAATG AAGGACTGAT GCCTCGGAGT CTGTCCTTTT CCGGCCCTCG GTACAGCCGC 
AGCAGGACGC CACCCTCTGC GGCCAGCAGC AGGTACAGCA TGAGGAACCG CATCCAAAGC 
AGCCCCATGA CAGTCATCTC TGAGGGAGAG GGAGAGCCTG CCGAGCCCGC AGACAACAAA 
GTACGCAGGA CCCTGGACAC TACGAGGGGA TGCTCTCTGG ACAGACTGGC GGGACAGGAG 
ACGGACCGGG GCAGCCCGCT GTGTAGCGAG GAGCCTGCCT CTGTGGATGG GATAGGAAGG 
GCTAAGGACG ACGGTCCCTC GGAGCAGTGT AGGGGTCCTG AACAGAAGAG TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006222893.1
CDS	2861..7774
Translation	MKKEGSAGSFRIKANPGSLSRAVSWINFSSLSRQTKRLFRSDGELSVCGQQVEADDENWIYRTQPRKAVSNLDEESRWTVHYTAPWHQQENVFLPSTRPPCVEDLHRQAKLNLKSVLRECDKLRRDGCRSSQYYSQGPTFAASSSPGDDYQEEDAETDRKYSLSSSEEERFIGIRRPKTPTSGDFSDLHTQTNWTKSLPLPTPEEKTRQQAQTVQADVVPINITGENFDRQASLRRSLIYTDTLVRRPKKVKRRKTISGIPDIIQKELASATGQEDDGSTHSLYSPDHYSTLGRLDSYRSTGQCLETRDSGCQTEEVKVIPPSVRRIRAHKGLGIAAQMSHLSGSSGNMSVLSDSAGIVFPSRLNNDTGFHSLPRAGARASTYSLEARMGALGSAEDTDDTFPYRGGSSQGPENFAHLGGASSTGMLSRPKSQQLRLLESPACVVSPHAAYSTSVIPNATLLSSSEVIVIHTAQSAGQLDSRVPGSSSYSKIKPRDHPTPRCSARDERQSPRHHWNEGHIIHSQALASAAPGATALLSLRDSEVSLNAPAINRENGSQAILYHCRSNLAFPAHPPDVDGKSECSSSGDRGCGSSESWEYKASSNGRASPLKPHLATPGCSTPTSNMSSCSLDQASLKEDARSLYSEDHDGYYMTAQGDSRHEAGSAYGISDGFGNPRHSTVNVFDGRAQRSQGDQATQQDRILSRNISLKKAKKPPPPPSRTDSLRRIPKKNNQTNGQVLNESLIASLQHSLQLSLPGQGSGSPSQSPCSDCEEPWLPRSRSQSIVSEGSSLTSTTTPNVYSLCGLTPSQSDTSSVKSEYADPWGYYIDYTTLQGDPGNPTGGCPASSEAATGNGPVRHIQEGSRAPVPQVPGCSVKPKITSPEKSQRVTSPSSGYSSQSNTPTALTPVPVFLKSMSPANGKGKAKPKVPERKSSLISSVSISSSSTSLSSNTSTEGSGTVKKLDSALASALAPPPPPLPALPSPSLANKSPFLPPPPPLADCSEGSPLPPSPVFPPPPPEALVPFCSPTDRCLSPSPTAFSPPLPRCSPPLPAPPPFLPPSEPPPAPPLDPKFMKENRPFIKNSSQSESSREALRRPANKEEGCRPSMPLITTEALQMVQLRPVRKNSGAETVLFSEPSAQEPRTPTAPQYHLKPSAFLKSRNSINEMESESQAASVTSSLPMPAKSQSQGDHDSAVERGGLQSCSDGAPGPGPSLRTALLPDSSPSRKPPPISKKPKLFLVVPPPQRDFTAEPTENGSEAFSGVPSPTGAEGEAGQSQEEKSSPASQAGSHATAPTSGSTVLERGTAGSLSPGVVEANAPMVQPSTSPGPAQEEAGENRVDGERNVKSCASPRGGEAGLPEPNTAGPSSDTADVSKEGGNDEVLTPTRPRTTEDLFAAIHRSKRKVLGRKDSEDDHTRNHSPSPPVTPTGAAPSLASPKQVGSIQRSLKKSSTSSDNFKALLLKKGSRSDTSARMSAAEMLKSTDPRFQRSRSEPSPEVPDSPSSFSPNKNRRAPEEWAKNEGLMPRSLSFSGPRYSRSRTPPSAASSRYSMRNRIQSSPMTVISEGEGEPAEPADNKVRRTLDTTRGCSLDRLAGQETDRGSPLCSEEPASVDGIGRAKDDGPSEQCRGPEQKS

Target ORF information:

RefSeq Version	XM_006222831.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus NHS-like 1 (Nhsl1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006222831.3

ORF Insert Sequence:

ATGAAAAAGG AAGGCTCTGC AGGTTCCTTC AGAATCAAGG CAAACCCAGG CTCCCTCTCA 
CGTGCCGTGA GTTGGATAAA TTTCTCCTCC CTGTCCAGGC AGACAAAGAG GCTGTTTCGC 
AGCGATGGAG AGCTGTCCGT TTGTGGGCAG CAGGTGGAGG CTGATGATGA GAATTGGATA 
TACAGGACCC AGCCCAGAAA AGCGGTTTCC AACTTAGACG AGGAGAGCCG ATGGACCGTC 
CACTATACGG CGCCGTGGCA CCAGCAGGAG AATGTGTTCC TCCCCTCCAC AAGACCCCCC 
TGTGTTGAGG ACCTGCACCG CCAAGCGAAG CTCAACCTCA AATCAGTGCT GAGGGAATGT 
GACAAGCTGA GGCGCGATGG CTGCCGAAGT TCTCAGTACT ATTCTCAGGG ACCCACCTTT 
GCAGCCAGCT CCAGCCCAGG CGATGACTAT CAAGAGGAAG ATGCAGAAAC AGACCGAAAG 
TATTCTCTCT CTTCATCTGA AGAAGAAAGA TTTATTGGTA TCAGGAGACC TAAAACGCCA 
ACCTCAGGTG ACTTCTCTGA CCTTCATACT CAGACAAACT GGACCAAGTC CCTGCCGCTG 
CCAACACCAG AAGAGAAGAC ACGACAGCAA GCCCAGACAG TCCAGGCTGA TGTGGTTCCT 
ATTAACATAA CTGGGGAGAA TTTTGATCGT CAGGCCAGCC TTCGGCGGTC TCTAATTTAC 
ACTGACACTC TGGTAAGACG ACCGAAGAAA GTCAAAAGGA GAAAGACTAT TTCAGGAATC 
CCTGACATCA TTCAGAAGGA GCTAGCGTCA GCCACGGGCC AAGAGGATGA TGGTAGCACC 
CATTCTCTAT ACTCCCCAGA CCACTACTCT ACATTAGGAA GGCTTGATAG CTATCGGTCC 
ACGGGGCAAT GCTTGGAAAC CAGGGACTCT GGCTGTCAGA CAGAAGAAGT GAAAGTCATC 
CCACCGTCAG TGAGAAGGAT CAGGGCTCAC AAGGGGCTGG GCATTGCTGC CCAGATGAGC 
CACCTCTCGG GTTCCTCTGG CAACATGTCT GTGCTGAGCG ACTCGGCTGG TATCGTGTTC 
CCATCTCGCC TCAATAACGA TACTGGTTTC CACAGCCTTC CACGCGCTGG CGCAAGGGCA 
AGCACGTATT CCCTGGAGGC AAGGATGGGT GCCCTGGGCT CTGCTGAAGA TACGGATGAT 
ACTTTTCCCT ACCGAGGGGG TAGCTCCCAG GGGCCTGAAA ACTTTGCACA TTTAGGGGGT 
GCCTCGAGTA CCGGGATGCT TTCGAGGCCC AAATCCCAGC AGCTGAGGCT CTTGGAGAGC 
CCAGCGTGTG TGGTTTCACC GCACGCGGCC TACTCTACCA GCGTCATCCC AAACGCCACA 
CTGCTGTCCT CTTCGGAGGT TATTGTCATC CACACCGCTC AGAGTGCAGG ACAGCTGGAC 
AGTAGAGTCC CCGGCTCATC CTCATACTCA AAGATAAAAC CCAGAGACCA TCCCACACCC 
AGGTGCTCTG CGAGAGATGA GCGCCAGTCC CCACGACATC ACTGGAACGA GGGTCATATC 
ATTCACTCAC AGGCTTTAGC CTCCGCTGCC CCCGGTGCCA CCGCACTGTT ATCCCTCCGT 
GACTCAGAGG TCTCTCTCAA TGCCCCAGCT ATCAATAGGG AGAACGGGTC CCAAGCCATA 
CTTTATCACT GTAGAAGCAA CCTGGCCTTT CCTGCCCACC CTCCGGATGT GGATGGCAAG 
AGCGAGTGCA GTTCTTCAGG GGACAGGGGA TGTGGCAGTT CTGAGTCCTG GGAATACAAA 
GCCTCCAGCA ATGGGCGGGC TTCCCCACTG AAGCCGCACC TTGCGACTCC CGGTTGCTCT 
ACCCCCACAA GTAACATGAG CAGCTGCAGC TTGGACCAAG CATCCCTCAA GGAGGATGCC 
AGGTCCCTGT ATTCAGAGGA CCACGATGGT TACTATATGA CCGCCCAGGG TGATTCTAGA 
CACGAGGCTG GGAGCGCGTA CGGTATCAGT GATGGCTTTG GAAACCCCAG GCACAGCACG 
GTCAATGTTT TTGACGGAAG GGCTCAGAGA AGCCAAGGGG ATCAGGCCAC TCAGCAGGAT 
AGAATCCTCT CAAGAAACAT CTCTCTGAAG AAAGCAAAAA AACCACCACC GCCACCATCC 
CGGACAGATT CTCTGCGAAG GATTCCCAAG AAGAACAACC AGACAAATGG GCAGGTGCTC 
AACGAGAGCC TGATCGCCTC ACTCCAGCAT TCTCTGCAGC TAAGCCTCCC TGGCCAAGGC 
AGCGGTTCCC CTTCCCAGAG CCCCTGCAGT GACTGTGAGG AACCCTGGCT CCCCAGGTCC 
AGAAGCCAGA GCATTGTTAG CGAGGGTAGT AGCTTGACCT CCACCACCAC TCCCAACGTG 
TATTCTCTGT GCGGGCTCAC TCCATCTCAG AGCGACACAA GCAGCGTCAA GTCGGAATAC 
GCAGACCCCT GGGGCTACTA CATTGACTAC ACAACTTTGC AGGGAGATCC AGGGAACCCC 
ACAGGGGGCT GCCCAGCCAG TAGTGAGGCA GCCACTGGAA ATGGGCCAGT GCGCCACATC 
CAGGAGGGGT CGAGAGCCCC AGTGCCCCAA GTGCCGGGCT GCTCCGTGAA ACCAAAGATC 
ACCTCACCGG AGAAGTCACA GAGAGTCACC TCTCCATCCA GTGGGTATTC TAGCCAGTCG 
AATACACCCA CAGCACTCAC CCCCGTGCCT GTGTTTTTAA AATCGATGTC ACCAGCAAAT 
GGGAAGGGGA AGGCCAAGCC CAAGGTACCA GAAAGAAAAT CGTCTCTGAT CTCTTCAGTG 
TCCATCTCCT CATCGTCCAC TTCCCTCTCC TCTAATACCT CTACGGAAGG AAGTGGGACC 
GTGAAGAAAC TGGATTCAGC CCTGGCCTCG GCCCTTGCTC CTCCCCCTCC TCCTCTTCCC 
GCGCTGCCTT CTCCGAGTCT GGCAAACAAG TCCCCCTTCC TTCCACCTCC TCCTCCTCTA 
GCAGATTGCT CCGAGGGCTC TCCCTTACCG CCCTCTCCTG TGTTCCCCCC TCCACCACCA 
GAAGCCCTTG TTCCCTTCTG CTCCCCCACT GACAGGTGCC TTTCTCCTTC CCCCACAGCT 
TTTAGCCCCC CTCTTCCAAG ATGCTCACCT CCCTTGCCAG CACCTCCACC TTTCTTGCCC 
CCATCAGAAC CCCCACCTGC TCCGCCTCTG GACCCCAAAT TCATGAAAGA AAACAGGCCC 
TTTATCAAAA ACTCTAGCCA GTCCGAATCC TCTAGGGAGG CCCTCAGGCG GCCTGCCAAC 
AAGGAGGAGG GCTGCCGACC CTCGATGCCC CTGATCACCA CAGAAGCTTT GCAGATGGTG 
CAGTTGAGGC CAGTGAGGAA GAACTCAGGT GCTGAGACGG TCCTGTTTTC TGAACCATCA 
GCTCAGGAAC CACGAACCCC GACTGCTCCA CAGTATCACT TAAAGCCATC TGCTTTCCTC 
AAATCCCGAA ATAGCATAAA TGAAATGGAG AGTGAAAGCC AGGCTGCCTC TGTGACGAGC 
TCGCTTCCGA TGCCTGCCAA GAGCCAGAGT CAGGGTGACC ATGACAGTGC AGTCGAGCGT 
GGCGGCCTTC AGAGCTGCAG CGATGGAGCT CCAGGCCCGG GTCCCAGCCT CAGGACCGCT 
CTGCTCCCAG ACTCTTCACC CAGCAGGAAG CCACCCCCCA TCTCCAAGAA GCCCAAACTG 
TTCCTGGTGG TACCACCTCC GCAGAGAGAT TTCACAGCGG AGCCCACAGA GAACGGGAGC 
GAAGCTTTCT CAGGCGTGCC CAGCCCTACC GGGGCAGAGG GGGAAGCTGG GCAGAGCCAA 
GAGGAGAAAT CCAGCCCAGC ATCCCAAGCT GGCTCTCATG CTACGGCCCC CACCTCCGGC 
AGTACGGTTC TAGAAAGAGG AACTGCAGGG TCCTTGTCCC CTGGCGTTGT GGAAGCTAAT 
GCCCCCATGG TCCAACCTAG TACATCACCG GGCCCCGCAC AGGAAGAGGC AGGAGAGAAC 
CGCGTGGATG GTGAGAGAAA TGTGAAGAGC TGCGCGTCTC CACGGGGCGG AGAAGCTGGG 
TTGCCGGAAC CCAACACAGC TGGTCCGTCT TCAGACACGG CAGACGTTTC TAAAGAAGGA 
GGGAACGATG AGGTGCTGAC CCCTACCAGA CCCAGGACAA CGGAAGACCT GTTTGCAGCC 
ATTCACAGAT CCAAAAGGAA AGTCCTTGGT CGGAAGGACT CCGAAGATGA TCACACTCGA 
AATCATTCTC CCTCCCCGCC TGTGACACCC ACAGGTGCTG CCCCTAGCCT GGCCTCTCCA 
AAGCAAGTAG GGTCCATCCA GCGAAGCCTA AAAAAGAGCA GTACCAGCAG CGACAACTTC 
AAAGCTCTGC TGCTCAAGAA GGGGAGTCGC TCGGACACCA GCGCTCGCAT GTCCGCGGCG 
GAGATGCTCA AGTCCACGGA CCCTCGATTC CAGAGATCTA GGTCGGAGCC TTCGCCAGAG 
GTCCCGGACA GCCCATCCAG CTTCTCTCCC AATAAGAACA GGAGAGCTCC GGAGGAGTGG 
GCCAAGAATG AAGGACTGAT GCCTCGGAGT CTGTCCTTTT CCGGCCCTCG GTACAGCCGC 
AGCAGGACGC CACCCTCTGC GGCCAGCAGC AGGTACAGCA TGAGGAACCG CATCCAAAGC 
AGCCCCATGA CAGTCATCTC TGAGGGAGAG GGAGAGCCTG CCGAGCCCGC AGACAACAAA 
GTACGCAGGA CCCTGGACAC TACGAGGGGA TGCTCTCTGG ACAGACTGGC GGGACAGGAG 
ACGGACCGGG GCAGCCCGCT GTGTAGCGAG GAGCCTGCCT CTGTGGATGG GATAGGAAGG 
GCTAAGGACG ACGGTCCCTC GGAGCAGTGT AGGGGTCCTG AACAGAAGAG TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nhsl1 ( XM_006227668.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006227730.1 Rattus norvegicus NHS-like 1 (Nhsl1), transcript variant X2, mRNA.

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CloneID	ORa13975
Clone ID Related Accession (Same CDS sequence)	XM_006222831.3 , XM_006227668.3
Accession Version	XM_006227668.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4914bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	NHS-like protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006227668.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAAAGG AAGGCTCTGC AGGTTCCTTC AGAATCAAGG CAAACCCAGG CTCCCTCTCA 
CGTGCCGTGA GTTGGATAAA TTTCTCCTCC CTGTCCAGGC AGACAAAGAG GCTGTTTCGC 
AGCGATGGAG AGCTGTCCGT TTGTGGGCAG CAGGTGGAGG CTGATGATGA GAATTGGATA 
TACAGGACCC AGCCCAGAAA AGCGGTTTCC AACTTAGACG AGGAGAGCCG ATGGACCGTC 
CACTATACGG CGCCGTGGCA CCAGCAGGAG AATGTGTTCC TCCCCTCCAC AAGACCCCCC 
TGTGTTGAGG ACCTGCACCG CCAAGCGAAG CTCAACCTCA AATCAGTGCT GAGGGAATGT 
GACAAGCTGA GGCGCGATGG CTGCCGAAGT TCTCAGTACT ATTCTCAGGG ACCCACCTTT 
GCAGCCAGCT CCAGCCCAGG CGATGACTAT CAAGAGGAAG ATGCAGAAAC AGACCGAAAG 
TATTCTCTCT CTTCATCTGA AGAAGAAAGA TTTATTGGTA TCAGGAGACC TAAAACGCCA 
ACCTCAGGTG ACTTCTCTGA CCTTCATACT CAGACAAACT GGACCAAGTC CCTGCCGCTG 
CCAACACCAG AAGAGAAGAC ACGACAGCAA GCCCAGACAG TCCAGGCTGA TGTGGTTCCT 
ATTAACATAA CTGGGGAGAA TTTTGATCGT CAGGCCAGCC TTCGGCGGTC TCTAATTTAC 
ACTGACACTC TGGTAAGACG ACCGAAGAAA GTCAAAAGGA GAAAGACTAT TTCAGGAATC 
CCTGACATCA TTCAGAAGGA GCTAGCGTCA GCCACGGGCC AAGAGGATGA TGGTAGCACC 
CATTCTCTAT ACTCCCCAGA CCACTACTCT ACATTAGGAA GGCTTGATAG CTATCGGTCC 
ACGGGGCAAT GCTTGGAAAC CAGGGACTCT GGCTGTCAGA CAGAAGAAGT GAAAGTCATC 
CCACCGTCAG TGAGAAGGAT CAGGGCTCAC AAGGGGCTGG GCATTGCTGC CCAGATGAGC 
CACCTCTCGG GTTCCTCTGG CAACATGTCT GTGCTGAGCG ACTCGGCTGG TATCGTGTTC 
CCATCTCGCC TCAATAACGA TACTGGTTTC CACAGCCTTC CACGCGCTGG CGCAAGGGCA 
AGCACGTATT CCCTGGAGGC AAGGATGGGT GCCCTGGGCT CTGCTGAAGA TACGGATGAT 
ACTTTTCCCT ACCGAGGGGG TAGCTCCCAG GGGCCTGAAA ACTTTGCACA TTTAGGGGGT 
GCCTCGAGTA CCGGGATGCT TTCGAGGCCC AAATCCCAGC AGCTGAGGCT CTTGGAGAGC 
CCAGCGTGTG TGGTTTCACC GCACGCGGCC TACTCTACCA GCGTCATCCC AAACGCCACA 
CTGCTGTCCT CTTCGGAGGT TATTGTCATC CACACCGCTC AGAGTGCAGG ACAGCTGGAC 
AGTAGAGTCC CCGGCTCATC CTCATACTCA AAGATAAAAC CCAGAGACCA TCCCACACCC 
AGGTGCTCTG CGAGAGATGA GCGCCAGTCC CCACGACATC ACTGGAACGA GGGTCATATC 
ATTCACTCAC AGGCTTTAGC CTCCGCTGCC CCCGGTGCCA CCGCACTGTT ATCCCTCCGT 
GACTCAGAGG TCTCTCTCAA TGCCCCAGCT ATCAATAGGG AGAACGGGTC CCAAGCCATA 
CTTTATCACT GTAGAAGCAA CCTGGCCTTT CCTGCCCACC CTCCGGATGT GGATGGCAAG 
AGCGAGTGCA GTTCTTCAGG GGACAGGGGA TGTGGCAGTT CTGAGTCCTG GGAATACAAA 
GCCTCCAGCA ATGGGCGGGC TTCCCCACTG AAGCCGCACC TTGCGACTCC CGGTTGCTCT 
ACCCCCACAA GTAACATGAG CAGCTGCAGC TTGGACCAAG CATCCCTCAA GGAGGATGCC 
AGGTCCCTGT ATTCAGAGGA CCACGATGGT TACTATATGA CCGCCCAGGG TGATTCTAGA 
CACGAGGCTG GGAGCGCGTA CGGTATCAGT GATGGCTTTG GAAACCCCAG GCACAGCACG 
GTCAATGTTT TTGACGGAAG GGCTCAGAGA AGCCAAGGGG ATCAGGCCAC TCAGCAGGAT 
AGAATCCTCT CAAGAAACAT CTCTCTGAAG AAAGCAAAAA AACCACCACC GCCACCATCC 
CGGACAGATT CTCTGCGAAG GATTCCCAAG AAGAACAACC AGACAAATGG GCAGGTGCTC 
AACGAGAGCC TGATCGCCTC ACTCCAGCAT TCTCTGCAGC TAAGCCTCCC TGGCCAAGGC 
AGCGGTTCCC CTTCCCAGAG CCCCTGCAGT GACTGTGAGG AACCCTGGCT CCCCAGGTCC 
AGAAGCCAGA GCATTGTTAG CGAGGGTAGT AGCTTGACCT CCACCACCAC TCCCAACGTG 
TATTCTCTGT GCGGGCTCAC TCCATCTCAG AGCGACACAA GCAGCGTCAA GTCGGAATAC 
GCAGACCCCT GGGGCTACTA CATTGACTAC ACAACTTTGC AGGGAGATCC AGGGAACCCC 
ACAGGGGGCT GCCCAGCCAG TAGTGAGGCA GCCACTGGAA ATGGGCCAGT GCGCCACATC 
CAGGAGGGGT CGAGAGCCCC AGTGCCCCAA GTGCCGGGCT GCTCCGTGAA ACCAAAGATC 
ACCTCACCGG AGAAGTCACA GAGAGTCACC TCTCCATCCA GTGGGTATTC TAGCCAGTCG 
AATACACCCA CAGCACTCAC CCCCGTGCCT GTGTTTTTAA AATCGATGTC ACCAGCAAAT 
GGGAAGGGGA AGGCCAAGCC CAAGGTACCA GAAAGAAAAT CGTCTCTGAT CTCTTCAGTG 
TCCATCTCCT CATCGTCCAC TTCCCTCTCC TCTAATACCT CTACGGAAGG AAGTGGGACC 
GTGAAGAAAC TGGATTCAGC CCTGGCCTCG GCCCTTGCTC CTCCCCCTCC TCCTCTTCCC 
GCGCTGCCTT CTCCGAGTCT GGCAAACAAG TCCCCCTTCC TTCCACCTCC TCCTCCTCTA 
GCAGATTGCT CCGAGGGCTC TCCCTTACCG CCCTCTCCTG TGTTCCCCCC TCCACCACCA 
GAAGCCCTTG TTCCCTTCTG CTCCCCCACT GACAGGTGCC TTTCTCCTTC CCCCACAGCT 
TTTAGCCCCC CTCTTCCAAG ATGCTCACCT CCCTTGCCAG CACCTCCACC TTTCTTGCCC 
CCATCAGAAC CCCCACCTGC TCCGCCTCTG GACCCCAAAT TCATGAAAGA AAACAGGCCC 
TTTATCAAAA ACTCTAGCCA GTCCGAATCC TCTAGGGAGG CCCTCAGGCG GCCTGCCAAC 
AAGGAGGAGG GCTGCCGACC CTCGATGCCC CTGATCACCA CAGAAGCTTT GCAGATGGTG 
CAGTTGAGGC CAGTGAGGAA GAACTCAGGT GCTGAGACGG TCCTGTTTTC TGAACCATCA 
GCTCAGGAAC CACGAACCCC GACTGCTCCA CAGTATCACT TAAAGCCATC TGCTTTCCTC 
AAATCCCGAA ATAGCATAAA TGAAATGGAG AGTGAAAGCC AGGCTGCCTC TGTGACGAGC 
TCGCTTCCGA TGCCTGCCAA GAGCCAGAGT CAGGGTGACC ATGACAGTGC AGTCGAGCGT 
GGCGGCCTTC AGAGCTGCAG CGATGGAGCT CCAGGCCCGG GTCCCAGCCT CAGGACCGCT 
CTGCTCCCAG ACTCTTCACC CAGCAGGAAG CCACCCCCCA TCTCCAAGAA GCCCAAACTG 
TTCCTGGTGG TACCACCTCC GCAGAGAGAT TTCACAGCGG AGCCCACAGA GAACGGGAGC 
GAAGCTTTCT CAGGCGTGCC CAGCCCTACC GGGGCAGAGG GGGAAGCTGG GCAGAGCCAA 
GAGGAGAAAT CCAGCCCAGC ATCCCAAGCT GGCTCTCATG CTACGGCCCC CACCTCCGGC 
AGTACGGTTC TAGAAAGAGG AACTGCAGGG TCCTTGTCCC CTGGCGTTGT GGAAGCTAAT 
GCCCCCATGG TCCAACCTAG TACATCACCG GGCCCCGCAC AGGAAGAGGC AGGAGAGAAC 
CGCGTGGATG GTGAGAGAAA TGTGAAGAGC TGCGCGTCTC CACGGGGCGG AGAAGCTGGG 
TTGCCGGAAC CCAACACAGC TGGTCCGTCT TCAGACACGG CAGACGTTTC TAAAGAAGGA 
GGGAACGATG AGGTGCTGAC CCCTACCAGA CCCAGGACAA CGGAAGACCT GTTTGCAGCC 
ATTCACAGAT CCAAAAGGAA AGTCCTTGGT CGGAAGGACT CCGAAGATGA TCACACTCGA 
AATCATTCTC CCTCCCCGCC TGTGACACCC ACAGGTGCTG CCCCTAGCCT GGCCTCTCCA 
AAGCAAGTAG GGTCCATCCA GCGAAGCCTA AAAAAGAGCA GTACCAGCAG CGACAACTTC 
AAAGCTCTGC TGCTCAAGAA GGGGAGTCGC TCGGACACCA GCGCTCGCAT GTCCGCGGCG 
GAGATGCTCA AGTCCACGGA CCCTCGATTC CAGAGATCTA GGTCGGAGCC TTCGCCAGAG 
GTCCCGGACA GCCCATCCAG CTTCTCTCCC AATAAGAACA GGAGAGCTCC GGAGGAGTGG 
GCCAAGAATG AAGGACTGAT GCCTCGGAGT CTGTCCTTTT CCGGCCCTCG GTACAGCCGC 
AGCAGGACGC CACCCTCTGC GGCCAGCAGC AGGTACAGCA TGAGGAACCG CATCCAAAGC 
AGCCCCATGA CAGTCATCTC TGAGGGAGAG GGAGAGCCTG CCGAGCCCGC AGACAACAAA 
GTACGCAGGA CCCTGGACAC TACGAGGGGA TGCTCTCTGG ACAGACTGGC GGGACAGGAG 
ACGGACCGGG GCAGCCCGCT GTGTAGCGAG GAGCCTGCCT CTGTGGATGG GATAGGAAGG 
GCTAAGGACG ACGGTCCCTC GGAGCAGTGT AGGGGTCCTG AACAGAAGAG TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006227730.1
CDS	2861..7774
Translation	MKKEGSAGSFRIKANPGSLSRAVSWINFSSLSRQTKRLFRSDGELSVCGQQVEADDENWIYRTQPRKAVSNLDEESRWTVHYTAPWHQQENVFLPSTRPPCVEDLHRQAKLNLKSVLRECDKLRRDGCRSSQYYSQGPTFAASSSPGDDYQEEDAETDRKYSLSSSEEERFIGIRRPKTPTSGDFSDLHTQTNWTKSLPLPTPEEKTRQQAQTVQADVVPINITGENFDRQASLRRSLIYTDTLVRRPKKVKRRKTISGIPDIIQKELASATGQEDDGSTHSLYSPDHYSTLGRLDSYRSTGQCLETRDSGCQTEEVKVIPPSVRRIRAHKGLGIAAQMSHLSGSSGNMSVLSDSAGIVFPSRLNNDTGFHSLPRAGARASTYSLEARMGALGSAEDTDDTFPYRGGSSQGPENFAHLGGASSTGMLSRPKSQQLRLLESPACVVSPHAAYSTSVIPNATLLSSSEVIVIHTAQSAGQLDSRVPGSSSYSKIKPRDHPTPRCSARDERQSPRHHWNEGHIIHSQALASAAPGATALLSLRDSEVSLNAPAINRENGSQAILYHCRSNLAFPAHPPDVDGKSECSSSGDRGCGSSESWEYKASSNGRASPLKPHLATPGCSTPTSNMSSCSLDQASLKEDARSLYSEDHDGYYMTAQGDSRHEAGSAYGISDGFGNPRHSTVNVFDGRAQRSQGDQATQQDRILSRNISLKKAKKPPPPPSRTDSLRRIPKKNNQTNGQVLNESLIASLQHSLQLSLPGQGSGSPSQSPCSDCEEPWLPRSRSQSIVSEGSSLTSTTTPNVYSLCGLTPSQSDTSSVKSEYADPWGYYIDYTTLQGDPGNPTGGCPASSEAATGNGPVRHIQEGSRAPVPQVPGCSVKPKITSPEKSQRVTSPSSGYSSQSNTPTALTPVPVFLKSMSPANGKGKAKPKVPERKSSLISSVSISSSSTSLSSNTSTEGSGTVKKLDSALASALAPPPPPLPALPSPSLANKSPFLPPPPPLADCSEGSPLPPSPVFPPPPPEALVPFCSPTDRCLSPSPTAFSPPLPRCSPPLPAPPPFLPPSEPPPAPPLDPKFMKENRPFIKNSSQSESSREALRRPANKEEGCRPSMPLITTEALQMVQLRPVRKNSGAETVLFSEPSAQEPRTPTAPQYHLKPSAFLKSRNSINEMESESQAASVTSSLPMPAKSQSQGDHDSAVERGGLQSCSDGAPGPGPSLRTALLPDSSPSRKPPPISKKPKLFLVVPPPQRDFTAEPTENGSEAFSGVPSPTGAEGEAGQSQEEKSSPASQAGSHATAPTSGSTVLERGTAGSLSPGVVEANAPMVQPSTSPGPAQEEAGENRVDGERNVKSCASPRGGEAGLPEPNTAGPSSDTADVSKEGGNDEVLTPTRPRTTEDLFAAIHRSKRKVLGRKDSEDDHTRNHSPSPPVTPTGAAPSLASPKQVGSIQRSLKKSSTSSDNFKALLLKKGSRSDTSARMSAAEMLKSTDPRFQRSRSEPSPEVPDSPSSFSPNKNRRAPEEWAKNEGLMPRSLSFSGPRYSRSRTPPSAASSRYSMRNRIQSSPMTVISEGEGEPAEPADNKVRRTLDTTRGCSLDRLAGQETDRGSPLCSEEPASVDGIGRAKDDGPSEQCRGPEQKS

Target ORF information:

RefSeq Version	XM_006227668.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus NHS-like 1 (Nhsl1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006227668.3

ORF Insert Sequence:

ATGAAAAAGG AAGGCTCTGC AGGTTCCTTC AGAATCAAGG CAAACCCAGG CTCCCTCTCA 
CGTGCCGTGA GTTGGATAAA TTTCTCCTCC CTGTCCAGGC AGACAAAGAG GCTGTTTCGC 
AGCGATGGAG AGCTGTCCGT TTGTGGGCAG CAGGTGGAGG CTGATGATGA GAATTGGATA 
TACAGGACCC AGCCCAGAAA AGCGGTTTCC AACTTAGACG AGGAGAGCCG ATGGACCGTC 
CACTATACGG CGCCGTGGCA CCAGCAGGAG AATGTGTTCC TCCCCTCCAC AAGACCCCCC 
TGTGTTGAGG ACCTGCACCG CCAAGCGAAG CTCAACCTCA AATCAGTGCT GAGGGAATGT 
GACAAGCTGA GGCGCGATGG CTGCCGAAGT TCTCAGTACT ATTCTCAGGG ACCCACCTTT 
GCAGCCAGCT CCAGCCCAGG CGATGACTAT CAAGAGGAAG ATGCAGAAAC AGACCGAAAG 
TATTCTCTCT CTTCATCTGA AGAAGAAAGA TTTATTGGTA TCAGGAGACC TAAAACGCCA 
ACCTCAGGTG ACTTCTCTGA CCTTCATACT CAGACAAACT GGACCAAGTC CCTGCCGCTG 
CCAACACCAG AAGAGAAGAC ACGACAGCAA GCCCAGACAG TCCAGGCTGA TGTGGTTCCT 
ATTAACATAA CTGGGGAGAA TTTTGATCGT CAGGCCAGCC TTCGGCGGTC TCTAATTTAC 
ACTGACACTC TGGTAAGACG ACCGAAGAAA GTCAAAAGGA GAAAGACTAT TTCAGGAATC 
CCTGACATCA TTCAGAAGGA GCTAGCGTCA GCCACGGGCC AAGAGGATGA TGGTAGCACC 
CATTCTCTAT ACTCCCCAGA CCACTACTCT ACATTAGGAA GGCTTGATAG CTATCGGTCC 
ACGGGGCAAT GCTTGGAAAC CAGGGACTCT GGCTGTCAGA CAGAAGAAGT GAAAGTCATC 
CCACCGTCAG TGAGAAGGAT CAGGGCTCAC AAGGGGCTGG GCATTGCTGC CCAGATGAGC 
CACCTCTCGG GTTCCTCTGG CAACATGTCT GTGCTGAGCG ACTCGGCTGG TATCGTGTTC 
CCATCTCGCC TCAATAACGA TACTGGTTTC CACAGCCTTC CACGCGCTGG CGCAAGGGCA 
AGCACGTATT CCCTGGAGGC AAGGATGGGT GCCCTGGGCT CTGCTGAAGA TACGGATGAT 
ACTTTTCCCT ACCGAGGGGG TAGCTCCCAG GGGCCTGAAA ACTTTGCACA TTTAGGGGGT 
GCCTCGAGTA CCGGGATGCT TTCGAGGCCC AAATCCCAGC AGCTGAGGCT CTTGGAGAGC 
CCAGCGTGTG TGGTTTCACC GCACGCGGCC TACTCTACCA GCGTCATCCC AAACGCCACA 
CTGCTGTCCT CTTCGGAGGT TATTGTCATC CACACCGCTC AGAGTGCAGG ACAGCTGGAC 
AGTAGAGTCC CCGGCTCATC CTCATACTCA AAGATAAAAC CCAGAGACCA TCCCACACCC 
AGGTGCTCTG CGAGAGATGA GCGCCAGTCC CCACGACATC ACTGGAACGA GGGTCATATC 
ATTCACTCAC AGGCTTTAGC CTCCGCTGCC CCCGGTGCCA CCGCACTGTT ATCCCTCCGT 
GACTCAGAGG TCTCTCTCAA TGCCCCAGCT ATCAATAGGG AGAACGGGTC CCAAGCCATA 
CTTTATCACT GTAGAAGCAA CCTGGCCTTT CCTGCCCACC CTCCGGATGT GGATGGCAAG 
AGCGAGTGCA GTTCTTCAGG GGACAGGGGA TGTGGCAGTT CTGAGTCCTG GGAATACAAA 
GCCTCCAGCA ATGGGCGGGC TTCCCCACTG AAGCCGCACC TTGCGACTCC CGGTTGCTCT 
ACCCCCACAA GTAACATGAG CAGCTGCAGC TTGGACCAAG CATCCCTCAA GGAGGATGCC 
AGGTCCCTGT ATTCAGAGGA CCACGATGGT TACTATATGA CCGCCCAGGG TGATTCTAGA 
CACGAGGCTG GGAGCGCGTA CGGTATCAGT GATGGCTTTG GAAACCCCAG GCACAGCACG 
GTCAATGTTT TTGACGGAAG GGCTCAGAGA AGCCAAGGGG ATCAGGCCAC TCAGCAGGAT 
AGAATCCTCT CAAGAAACAT CTCTCTGAAG AAAGCAAAAA AACCACCACC GCCACCATCC 
CGGACAGATT CTCTGCGAAG GATTCCCAAG AAGAACAACC AGACAAATGG GCAGGTGCTC 
AACGAGAGCC TGATCGCCTC ACTCCAGCAT TCTCTGCAGC TAAGCCTCCC TGGCCAAGGC 
AGCGGTTCCC CTTCCCAGAG CCCCTGCAGT GACTGTGAGG AACCCTGGCT CCCCAGGTCC 
AGAAGCCAGA GCATTGTTAG CGAGGGTAGT AGCTTGACCT CCACCACCAC TCCCAACGTG 
TATTCTCTGT GCGGGCTCAC TCCATCTCAG AGCGACACAA GCAGCGTCAA GTCGGAATAC 
GCAGACCCCT GGGGCTACTA CATTGACTAC ACAACTTTGC AGGGAGATCC AGGGAACCCC 
ACAGGGGGCT GCCCAGCCAG TAGTGAGGCA GCCACTGGAA ATGGGCCAGT GCGCCACATC 
CAGGAGGGGT CGAGAGCCCC AGTGCCCCAA GTGCCGGGCT GCTCCGTGAA ACCAAAGATC 
ACCTCACCGG AGAAGTCACA GAGAGTCACC TCTCCATCCA GTGGGTATTC TAGCCAGTCG 
AATACACCCA CAGCACTCAC CCCCGTGCCT GTGTTTTTAA AATCGATGTC ACCAGCAAAT 
GGGAAGGGGA AGGCCAAGCC CAAGGTACCA GAAAGAAAAT CGTCTCTGAT CTCTTCAGTG 
TCCATCTCCT CATCGTCCAC TTCCCTCTCC TCTAATACCT CTACGGAAGG AAGTGGGACC 
GTGAAGAAAC TGGATTCAGC CCTGGCCTCG GCCCTTGCTC CTCCCCCTCC TCCTCTTCCC 
GCGCTGCCTT CTCCGAGTCT GGCAAACAAG TCCCCCTTCC TTCCACCTCC TCCTCCTCTA 
GCAGATTGCT CCGAGGGCTC TCCCTTACCG CCCTCTCCTG TGTTCCCCCC TCCACCACCA 
GAAGCCCTTG TTCCCTTCTG CTCCCCCACT GACAGGTGCC TTTCTCCTTC CCCCACAGCT 
TTTAGCCCCC CTCTTCCAAG ATGCTCACCT CCCTTGCCAG CACCTCCACC TTTCTTGCCC 
CCATCAGAAC CCCCACCTGC TCCGCCTCTG GACCCCAAAT TCATGAAAGA AAACAGGCCC 
TTTATCAAAA ACTCTAGCCA GTCCGAATCC TCTAGGGAGG CCCTCAGGCG GCCTGCCAAC 
AAGGAGGAGG GCTGCCGACC CTCGATGCCC CTGATCACCA CAGAAGCTTT GCAGATGGTG 
CAGTTGAGGC CAGTGAGGAA GAACTCAGGT GCTGAGACGG TCCTGTTTTC TGAACCATCA 
GCTCAGGAAC CACGAACCCC GACTGCTCCA CAGTATCACT TAAAGCCATC TGCTTTCCTC 
AAATCCCGAA ATAGCATAAA TGAAATGGAG AGTGAAAGCC AGGCTGCCTC TGTGACGAGC 
TCGCTTCCGA TGCCTGCCAA GAGCCAGAGT CAGGGTGACC ATGACAGTGC AGTCGAGCGT 
GGCGGCCTTC AGAGCTGCAG CGATGGAGCT CCAGGCCCGG GTCCCAGCCT CAGGACCGCT 
CTGCTCCCAG ACTCTTCACC CAGCAGGAAG CCACCCCCCA TCTCCAAGAA GCCCAAACTG 
TTCCTGGTGG TACCACCTCC GCAGAGAGAT TTCACAGCGG AGCCCACAGA GAACGGGAGC 
GAAGCTTTCT CAGGCGTGCC CAGCCCTACC GGGGCAGAGG GGGAAGCTGG GCAGAGCCAA 
GAGGAGAAAT CCAGCCCAGC ATCCCAAGCT GGCTCTCATG CTACGGCCCC CACCTCCGGC 
AGTACGGTTC TAGAAAGAGG AACTGCAGGG TCCTTGTCCC CTGGCGTTGT GGAAGCTAAT 
GCCCCCATGG TCCAACCTAG TACATCACCG GGCCCCGCAC AGGAAGAGGC AGGAGAGAAC 
CGCGTGGATG GTGAGAGAAA TGTGAAGAGC TGCGCGTCTC CACGGGGCGG AGAAGCTGGG 
TTGCCGGAAC CCAACACAGC TGGTCCGTCT TCAGACACGG CAGACGTTTC TAAAGAAGGA 
GGGAACGATG AGGTGCTGAC CCCTACCAGA CCCAGGACAA CGGAAGACCT GTTTGCAGCC 
ATTCACAGAT CCAAAAGGAA AGTCCTTGGT CGGAAGGACT CCGAAGATGA TCACACTCGA 
AATCATTCTC CCTCCCCGCC TGTGACACCC ACAGGTGCTG CCCCTAGCCT GGCCTCTCCA 
AAGCAAGTAG GGTCCATCCA GCGAAGCCTA AAAAAGAGCA GTACCAGCAG CGACAACTTC 
AAAGCTCTGC TGCTCAAGAA GGGGAGTCGC TCGGACACCA GCGCTCGCAT GTCCGCGGCG 
GAGATGCTCA AGTCCACGGA CCCTCGATTC CAGAGATCTA GGTCGGAGCC TTCGCCAGAG 
GTCCCGGACA GCCCATCCAG CTTCTCTCCC AATAAGAACA GGAGAGCTCC GGAGGAGTGG 
GCCAAGAATG AAGGACTGAT GCCTCGGAGT CTGTCCTTTT CCGGCCCTCG GTACAGCCGC 
AGCAGGACGC CACCCTCTGC GGCCAGCAGC AGGTACAGCA TGAGGAACCG CATCCAAAGC 
AGCCCCATGA CAGTCATCTC TGAGGGAGAG GGAGAGCCTG CCGAGCCCGC AGACAACAAA 
GTACGCAGGA CCCTGGACAC TACGAGGGGA TGCTCTCTGG ACAGACTGGC GGGACAGGAG 
ACGGACCGGG GCAGCCCGCT GTGTAGCGAG GAGCCTGCCT CTGTGGATGG GATAGGAAGG 
GCTAAGGACG ACGGTCCCTC GGAGCAGTGT AGGGGTCCTG AACAGAAGAG TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nhsl1 ( XM_002728626.5 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_002728672.1 Rattus norvegicus NHS-like 1 (Nhsl1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ORa13977
Clone ID Related Accession (Same CDS sequence)	XM_002728626.5 , XM_002725450.5
Accession Version	XM_002728626.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4782bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	NHS-like protein 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_002728626.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAAAGG AAGGCTCTGC AGGTTCCTTC AGAATCAAGG CAAACCCAGG CTCCCTCTCA 
CGTGCCGTGA GTTGGATAAA TTTCTCCTCC CTGTCCAGGC AGACAAAGAG GCTGTTTCGC 
AGCGATGGAG AGCTGTCCGT TTGTGGGCAG CAGGTGGAGG CTGATGATGA GAATTGGATA 
TACAGGACCC AGCCCAGAAA AGCGGTTTCC AACTTAGACG AGGAGAGCCG ATGGACCGTC 
CACTATACGG CGCCGTGGCA CCAGCAGGAG AATGTGTTCC TCCCCTCCAC AAGACCCCCC 
TGTGTTGAGG ACCTGCACCG CCAAGCGAAG CTCAACCTCA AATCAGTGCT GAGGGAATGT 
GACAAGCTGA GGCGCGATGG CTGCCGAAGT TCTCAGTACT ATTCTCAGGG ACCCACCTTT 
GCAGCCAGCT CCAGCCCAGG CGATGACTAT CAAGAGGAAG ATGCAGAAAC AGACCGAAAG 
TATTCTCTCT CTTCATCTGA AGAAGAAAGA TTTATTGGTA TCAGGAGACC TAAAACGCCA 
ACCTCAGGTG ACTTCTCTGA CCTTCATACT CAGACAAACT GGACCAAGTC CCTGCCGCTG 
CCAACACCAG AAGAGAAGAC ACGACAGCAA GCCCAGACAG TCCAGGCTGA TGTGGTTCCT 
ATTAACATAA CTGCGTCAGC CACGGGCCAA GAGGATGATG GTAGCACCCA TTCTCTATAC 
TCCCCAGACC ACTACTCTAC ATTAGGAAGG CTTGATAGCT ATCGGTCCAC GGGGCAATGC 
TTGGAAACCA GGGACTCTGG CTGTCAGACA GAAGAAGTGA AAGTCATCCC ACCGTCAGTG 
AGAAGGATCA GGGCTCACAA GGGGCTGGGC ATTGCTGCCC AGATGAGCCA CCTCTCGGGT 
TCCTCTGGCA ACATGTCTGT GCTGAGCGAC TCGGCTGGTA TCGTGTTCCC ATCTCGCCTC 
AATAACGATA CTGGTTTCCA CAGCCTTCCA CGCGCTGGCG CAAGGGCAAG CACGTATTCC 
CTGGAGGCAA GGATGGGTGC CCTGGGCTCT GCTGAAGATA CGGATGATAC TTTTCCCTAC 
CGAGGGGGTA GCTCCCAGGG GCCTGAAAAC TTTGCACATT TAGGGGGTGC CTCGAGTACC 
GGGATGCTTT CGAGGCCCAA ATCCCAGCAG CTGAGGCTCT TGGAGAGCCC AGCGTGTGTG 
GTTTCACCGC ACGCGGCCTA CTCTACCAGC GTCATCCCAA ACGCCACACT GCTGTCCTCT 
TCGGAGGTTA TTGTCATCCA CACCGCTCAG AGTGCAGGAC AGCTGGACAG TAGAGTCCCC 
GGCTCATCCT CATACTCAAA GATAAAACCC AGAGACCATC CCACACCCAG GTGCTCTGCG 
AGAGATGAGC GCCAGTCCCC ACGACATCAC TGGAACGAGG GTCATATCAT TCACTCACAG 
GCTTTAGCCT CCGCTGCCCC CGGTGCCACC GCACTGTTAT CCCTCCGTGA CTCAGAGGTC 
TCTCTCAATG CCCCAGCTAT CAATAGGGAG AACGGGTCCC AAGCCATACT TTATCACTGT 
AGAAGCAACC TGGCCTTTCC TGCCCACCCT CCGGATGTGG ATGGCAAGAG CGAGTGCAGT 
TCTTCAGGGG ACAGGGGATG TGGCAGTTCT GAGTCCTGGG AATACAAAGC CTCCAGCAAT 
GGGCGGGCTT CCCCACTGAA GCCGCACCTT GCGACTCCCG GTTGCTCTAC CCCCACAAGT 
AACATGAGCA GCTGCAGCTT GGACCAAGCA TCCCTCAAGG AGGATGCCAG GTCCCTGTAT 
TCAGAGGACC ACGATGGTTA CTATATGACC GCCCAGGGTG ATTCTAGACA CGAGGCTGGG 
AGCGCGTACG GTATCAGTGA TGGCTTTGGA AACCCCAGGC ACAGCACGGT CAATGTTTTT 
GACGGAAGGG CTCAGAGAAG CCAAGGGGAT CAGGCCACTC AGCAGGATAG AATCCTCTCA 
AGAAACATCT CTCTGAAGAA AGCAAAAAAA CCACCACCGC CACCATCCCG GACAGATTCT 
CTGCGAAGGA TTCCCAAGAA GAACAACCAG ACAAATGGGC AGGTGCTCAA CGAGAGCCTG 
ATCGCCTCAC TCCAGCATTC TCTGCAGCTA AGCCTCCCTG GCCAAGGCAG CGGTTCCCCT 
TCCCAGAGCC CCTGCAGTGA CTGTGAGGAA CCCTGGCTCC CCAGGTCCAG AAGCCAGAGC 
ATTGTTAGCG AGGGTAGTAG CTTGACCTCC ACCACCACTC CCAACGTGTA TTCTCTGTGC 
GGGCTCACTC CATCTCAGAG CGACACAAGC AGCGTCAAGT CGGAATACGC AGACCCCTGG 
GGCTACTACA TTGACTACAC AACTTTGCAG GGAGATCCAG GGAACCCCAC AGGGGGCTGC 
CCAGCCAGTA GTGAGGCAGC CACTGGAAAT GGGCCAGTGC GCCACATCCA GGAGGGGTCG 
AGAGCCCCAG TGCCCCAAGT GCCGGGCTGC TCCGTGAAAC CAAAGATCAC CTCACCGGAG 
AAGTCACAGA GAGTCACCTC TCCATCCAGT GGGTATTCTA GCCAGTCGAA TACACCCACA 
GCACTCACCC CCGTGCCTGT GTTTTTAAAA TCGATGTCAC CAGCAAATGG GAAGGGGAAG 
GCCAAGCCCA AGGTACCAGA AAGAAAATCG TCTCTGATCT CTTCAGTGTC CATCTCCTCA 
TCGTCCACTT CCCTCTCCTC TAATACCTCT ACGGAAGGAA GTGGGACCGT GAAGAAACTG 
GATTCAGCCC TGGCCTCGGC CCTTGCTCCT CCCCCTCCTC CTCTTCCCGC GCTGCCTTCT 
CCGAGTCTGG CAAACAAGTC CCCCTTCCTT CCACCTCCTC CTCCTCTAGC AGATTGCTCC 
GAGGGCTCTC CCTTACCGCC CTCTCCTGTG TTCCCCCCTC CACCACCAGA AGCCCTTGTT 
CCCTTCTGCT CCCCCACTGA CAGGTGCCTT TCTCCTTCCC CCACAGCTTT TAGCCCCCCT 
CTTCCAAGAT GCTCACCTCC CTTGCCAGCA CCTCCACCTT TCTTGCCCCC ATCAGAACCC 
CCACCTGCTC CGCCTCTGGA CCCCAAATTC ATGAAAGAAA ACAGGCCCTT TATCAAAAAC 
TCTAGCCAGT CCGAATCCTC TAGGGAGGCC CTCAGGCGGC CTGCCAACAA GGAGGAGGGC 
TGCCGACCCT CGATGCCCCT GATCACCACA GAAGCTTTGC AGATGGTGCA GTTGAGGCCA 
GTGAGGAAGA ACTCAGGTGC TGAGACGGTC CTGTTTTCTG AACCATCAGC TCAGGAACCA 
CGAACCCCGA CTGCTCCACA GTATCACTTA AAGCCATCTG CTTTCCTCAA ATCCCGAAAT 
AGCATAAATG AAATGGAGAG TGAAAGCCAG GCTGCCTCTG TGACGAGCTC GCTTCCGATG 
CCTGCCAAGA GCCAGAGTCA GGGTGACCAT GACAGTGCAG TCGAGCGTGG CGGCCTTCAG 
AGCTGCAGCG ATGGAGCTCC AGGCCCGGGT CCCAGCCTCA GGACCGCTCT GCTCCCAGAC 
TCTTCACCCA GCAGGAAGCC ACCCCCCATC TCCAAGAAGC CCAAACTGTT CCTGGTGGTA 
CCACCTCCGC AGAGAGATTT CACAGCGGAG CCCACAGAGA ACGGGAGCGA AGCTTTCTCA 
GGCGTGCCCA GCCCTACCGG GGCAGAGGGG GAAGCTGGGC AGAGCCAAGA GGAGAAATCC 
AGCCCAGCAT CCCAAGCTGG CTCTCATGCT ACGGCCCCCA CCTCCGGCAG TACGGTTCTA 
GAAAGAGGAA CTGCAGGGTC CTTGTCCCCT GGCGTTGTGG AAGCTAATGC CCCCATGGTC 
CAACCTAGTA CATCACCGGG CCCCGCACAG GAAGAGGCAG GAGAGAACCG CGTGGATGGT 
GAGAGAAATG TGAAGAGCTG CGCGTCTCCA CGGGGCGGAG AAGCTGGGTT GCCGGAACCC 
AACACAGCTG GTCCGTCTTC AGACACGGCA GACGTTTCTA AAGAAGGAGG GAACGATGAG 
GTGCTGACCC CTACCAGACC CAGGACAACG GAAGACCTGT TTGCAGCCAT TCACAGATCC 
AAAAGGAAAG TCCTTGGTCG GAAGGACTCC GAAGATGATC ACACTCGAAA TCATTCTCCC 
TCCCCGCCTG TGACACCCAC AGGTGCTGCC CCTAGCCTGG CCTCTCCAAA GCAAGTAGGG 
TCCATCCAGC GAAGCCTAAA AAAGAGCAGT ACCAGCAGCG ACAACTTCAA AGCTCTGCTG 
CTCAAGAAGG GGAGTCGCTC GGACACCAGC GCTCGCATGT CCGCGGCGGA GATGCTCAAG 
TCCACGGACC CTCGATTCCA GAGATCTAGG TCGGAGCCTT CGCCAGAGGT CCCGGACAGC 
CCATCCAGCT TCTCTCCCAA TAAGAACAGG AGAGCTCCGG AGGAGTGGGC CAAGAATGAA 
GGACTGATGC CTCGGAGTCT GTCCTTTTCC GGCCCTCGGT ACAGCCGCAG CAGGACGCCA 
CCCTCTGCGG CCAGCAGCAG GTACAGCATG AGGAACCGCA TCCAAAGCAG CCCCATGACA 
GTCATCTCTG AGGGAGAGGG AGAGCCTGCC GAGCCCGCAG ACAACAAAGT ACGCAGGACC 
CTGGACACTA CGAGGGGATG CTCTCTGGAC AGACTGGCGG GACAGGAGAC GGACCGGGGC 
AGCCCGCTGT GTAGCGAGGA GCCTGCCTCT GTGGATGGGA TAGGAAGGGC TAAGGACGAC 
GGTCCCTCGG AGCAGTGTAG GGGTCCTGAA CAGAAGAGTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002728672.1
CDS	2861..7642
Translation	MKKEGSAGSFRIKANPGSLSRAVSWINFSSLSRQTKRLFRSDGELSVCGQQVEADDENWIYRTQPRKAVSNLDEESRWTVHYTAPWHQQENVFLPSTRPPCVEDLHRQAKLNLKSVLRECDKLRRDGCRSSQYYSQGPTFAASSSPGDDYQEEDAETDRKYSLSSSEEERFIGIRRPKTPTSGDFSDLHTQTNWTKSLPLPTPEEKTRQQAQTVQADVVPINITASATGQEDDGSTHSLYSPDHYSTLGRLDSYRSTGQCLETRDSGCQTEEVKVIPPSVRRIRAHKGLGIAAQMSHLSGSSGNMSVLSDSAGIVFPSRLNNDTGFHSLPRAGARASTYSLEARMGALGSAEDTDDTFPYRGGSSQGPENFAHLGGASSTGMLSRPKSQQLRLLESPACVVSPHAAYSTSVIPNATLLSSSEVIVIHTAQSAGQLDSRVPGSSSYSKIKPRDHPTPRCSARDERQSPRHHWNEGHIIHSQALASAAPGATALLSLRDSEVSLNAPAINRENGSQAILYHCRSNLAFPAHPPDVDGKSECSSSGDRGCGSSESWEYKASSNGRASPLKPHLATPGCSTPTSNMSSCSLDQASLKEDARSLYSEDHDGYYMTAQGDSRHEAGSAYGISDGFGNPRHSTVNVFDGRAQRSQGDQATQQDRILSRNISLKKAKKPPPPPSRTDSLRRIPKKNNQTNGQVLNESLIASLQHSLQLSLPGQGSGSPSQSPCSDCEEPWLPRSRSQSIVSEGSSLTSTTTPNVYSLCGLTPSQSDTSSVKSEYADPWGYYIDYTTLQGDPGNPTGGCPASSEAATGNGPVRHIQEGSRAPVPQVPGCSVKPKITSPEKSQRVTSPSSGYSSQSNTPTALTPVPVFLKSMSPANGKGKAKPKVPERKSSLISSVSISSSSTSLSSNTSTEGSGTVKKLDSALASALAPPPPPLPALPSPSLANKSPFLPPPPPLADCSEGSPLPPSPVFPPPPPEALVPFCSPTDRCLSPSPTAFSPPLPRCSPPLPAPPPFLPPSEPPPAPPLDPKFMKENRPFIKNSSQSESSREALRRPANKEEGCRPSMPLITTEALQMVQLRPVRKNSGAETVLFSEPSAQEPRTPTAPQYHLKPSAFLKSRNSINEMESESQAASVTSSLPMPAKSQSQGDHDSAVERGGLQSCSDGAPGPGPSLRTALLPDSSPSRKPPPISKKPKLFLVVPPPQRDFTAEPTENGSEAFSGVPSPTGAEGEAGQSQEEKSSPASQAGSHATAPTSGSTVLERGTAGSLSPGVVEANAPMVQPSTSPGPAQEEAGENRVDGERNVKSCASPRGGEAGLPEPNTAGPSSDTADVSKEGGNDEVLTPTRPRTTEDLFAAIHRSKRKVLGRKDSEDDHTRNHSPSPPVTPTGAAPSLASPKQVGSIQRSLKKSSTSSDNFKALLLKKGSRSDTSARMSAAEMLKSTDPRFQRSRSEPSPEVPDSPSSFSPNKNRRAPEEWAKNEGLMPRSLSFSGPRYSRSRTPPSAASSRYSMRNRIQSSPMTVISEGEGEPAEPADNKVRRTLDTTRGCSLDRLAGQETDRGSPLCSEEPASVDGIGRAKDDGPSEQCRGPEQKS

Target ORF information:

RefSeq Version	XM_002728626.5
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus NHS-like 1 (Nhsl1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002728626.5

ORF Insert Sequence:

ATGAAAAAGG AAGGCTCTGC AGGTTCCTTC AGAATCAAGG CAAACCCAGG CTCCCTCTCA 
CGTGCCGTGA GTTGGATAAA TTTCTCCTCC CTGTCCAGGC AGACAAAGAG GCTGTTTCGC 
AGCGATGGAG AGCTGTCCGT TTGTGGGCAG CAGGTGGAGG CTGATGATGA GAATTGGATA 
TACAGGACCC AGCCCAGAAA AGCGGTTTCC AACTTAGACG AGGAGAGCCG ATGGACCGTC 
CACTATACGG CGCCGTGGCA CCAGCAGGAG AATGTGTTCC TCCCCTCCAC AAGACCCCCC 
TGTGTTGAGG ACCTGCACCG CCAAGCGAAG CTCAACCTCA AATCAGTGCT GAGGGAATGT 
GACAAGCTGA GGCGCGATGG CTGCCGAAGT TCTCAGTACT ATTCTCAGGG ACCCACCTTT 
GCAGCCAGCT CCAGCCCAGG CGATGACTAT CAAGAGGAAG ATGCAGAAAC AGACCGAAAG 
TATTCTCTCT CTTCATCTGA AGAAGAAAGA TTTATTGGTA TCAGGAGACC TAAAACGCCA 
ACCTCAGGTG ACTTCTCTGA CCTTCATACT CAGACAAACT GGACCAAGTC CCTGCCGCTG 
CCAACACCAG AAGAGAAGAC ACGACAGCAA GCCCAGACAG TCCAGGCTGA TGTGGTTCCT 
ATTAACATAA CTGCGTCAGC CACGGGCCAA GAGGATGATG GTAGCACCCA TTCTCTATAC 
TCCCCAGACC ACTACTCTAC ATTAGGAAGG CTTGATAGCT ATCGGTCCAC GGGGCAATGC 
TTGGAAACCA GGGACTCTGG CTGTCAGACA GAAGAAGTGA AAGTCATCCC ACCGTCAGTG 
AGAAGGATCA GGGCTCACAA GGGGCTGGGC ATTGCTGCCC AGATGAGCCA CCTCTCGGGT 
TCCTCTGGCA ACATGTCTGT GCTGAGCGAC TCGGCTGGTA TCGTGTTCCC ATCTCGCCTC 
AATAACGATA CTGGTTTCCA CAGCCTTCCA CGCGCTGGCG CAAGGGCAAG CACGTATTCC 
CTGGAGGCAA GGATGGGTGC CCTGGGCTCT GCTGAAGATA CGGATGATAC TTTTCCCTAC 
CGAGGGGGTA GCTCCCAGGG GCCTGAAAAC TTTGCACATT TAGGGGGTGC CTCGAGTACC 
GGGATGCTTT CGAGGCCCAA ATCCCAGCAG CTGAGGCTCT TGGAGAGCCC AGCGTGTGTG 
GTTTCACCGC ACGCGGCCTA CTCTACCAGC GTCATCCCAA ACGCCACACT GCTGTCCTCT 
TCGGAGGTTA TTGTCATCCA CACCGCTCAG AGTGCAGGAC AGCTGGACAG TAGAGTCCCC 
GGCTCATCCT CATACTCAAA GATAAAACCC AGAGACCATC CCACACCCAG GTGCTCTGCG 
AGAGATGAGC GCCAGTCCCC ACGACATCAC TGGAACGAGG GTCATATCAT TCACTCACAG 
GCTTTAGCCT CCGCTGCCCC CGGTGCCACC GCACTGTTAT CCCTCCGTGA CTCAGAGGTC 
TCTCTCAATG CCCCAGCTAT CAATAGGGAG AACGGGTCCC AAGCCATACT TTATCACTGT 
AGAAGCAACC TGGCCTTTCC TGCCCACCCT CCGGATGTGG ATGGCAAGAG CGAGTGCAGT 
TCTTCAGGGG ACAGGGGATG TGGCAGTTCT GAGTCCTGGG AATACAAAGC CTCCAGCAAT 
GGGCGGGCTT CCCCACTGAA GCCGCACCTT GCGACTCCCG GTTGCTCTAC CCCCACAAGT 
AACATGAGCA GCTGCAGCTT GGACCAAGCA TCCCTCAAGG AGGATGCCAG GTCCCTGTAT 
TCAGAGGACC ACGATGGTTA CTATATGACC GCCCAGGGTG ATTCTAGACA CGAGGCTGGG 
AGCGCGTACG GTATCAGTGA TGGCTTTGGA AACCCCAGGC ACAGCACGGT CAATGTTTTT 
GACGGAAGGG CTCAGAGAAG CCAAGGGGAT CAGGCCACTC AGCAGGATAG AATCCTCTCA 
AGAAACATCT CTCTGAAGAA AGCAAAAAAA CCACCACCGC CACCATCCCG GACAGATTCT 
CTGCGAAGGA TTCCCAAGAA GAACAACCAG ACAAATGGGC AGGTGCTCAA CGAGAGCCTG 
ATCGCCTCAC TCCAGCATTC TCTGCAGCTA AGCCTCCCTG GCCAAGGCAG CGGTTCCCCT 
TCCCAGAGCC CCTGCAGTGA CTGTGAGGAA CCCTGGCTCC CCAGGTCCAG AAGCCAGAGC 
ATTGTTAGCG AGGGTAGTAG CTTGACCTCC ACCACCACTC CCAACGTGTA TTCTCTGTGC 
GGGCTCACTC CATCTCAGAG CGACACAAGC AGCGTCAAGT CGGAATACGC AGACCCCTGG 
GGCTACTACA TTGACTACAC AACTTTGCAG GGAGATCCAG GGAACCCCAC AGGGGGCTGC 
CCAGCCAGTA GTGAGGCAGC CACTGGAAAT GGGCCAGTGC GCCACATCCA GGAGGGGTCG 
AGAGCCCCAG TGCCCCAAGT GCCGGGCTGC TCCGTGAAAC CAAAGATCAC CTCACCGGAG 
AAGTCACAGA GAGTCACCTC TCCATCCAGT GGGTATTCTA GCCAGTCGAA TACACCCACA 
GCACTCACCC CCGTGCCTGT GTTTTTAAAA TCGATGTCAC CAGCAAATGG GAAGGGGAAG 
GCCAAGCCCA AGGTACCAGA AAGAAAATCG TCTCTGATCT CTTCAGTGTC CATCTCCTCA 
TCGTCCACTT CCCTCTCCTC TAATACCTCT ACGGAAGGAA GTGGGACCGT GAAGAAACTG 
GATTCAGCCC TGGCCTCGGC CCTTGCTCCT CCCCCTCCTC CTCTTCCCGC GCTGCCTTCT 
CCGAGTCTGG CAAACAAGTC CCCCTTCCTT CCACCTCCTC CTCCTCTAGC AGATTGCTCC 
GAGGGCTCTC CCTTACCGCC CTCTCCTGTG TTCCCCCCTC CACCACCAGA AGCCCTTGTT 
CCCTTCTGCT CCCCCACTGA CAGGTGCCTT TCTCCTTCCC CCACAGCTTT TAGCCCCCCT 
CTTCCAAGAT GCTCACCTCC CTTGCCAGCA CCTCCACCTT TCTTGCCCCC ATCAGAACCC 
CCACCTGCTC CGCCTCTGGA CCCCAAATTC ATGAAAGAAA ACAGGCCCTT TATCAAAAAC 
TCTAGCCAGT CCGAATCCTC TAGGGAGGCC CTCAGGCGGC CTGCCAACAA GGAGGAGGGC 
TGCCGACCCT CGATGCCCCT GATCACCACA GAAGCTTTGC AGATGGTGCA GTTGAGGCCA 
GTGAGGAAGA ACTCAGGTGC TGAGACGGTC CTGTTTTCTG AACCATCAGC TCAGGAACCA 
CGAACCCCGA CTGCTCCACA GTATCACTTA AAGCCATCTG CTTTCCTCAA ATCCCGAAAT 
AGCATAAATG AAATGGAGAG TGAAAGCCAG GCTGCCTCTG TGACGAGCTC GCTTCCGATG 
CCTGCCAAGA GCCAGAGTCA GGGTGACCAT GACAGTGCAG TCGAGCGTGG CGGCCTTCAG 
AGCTGCAGCG ATGGAGCTCC AGGCCCGGGT CCCAGCCTCA GGACCGCTCT GCTCCCAGAC 
TCTTCACCCA GCAGGAAGCC ACCCCCCATC TCCAAGAAGC CCAAACTGTT CCTGGTGGTA 
CCACCTCCGC AGAGAGATTT CACAGCGGAG CCCACAGAGA ACGGGAGCGA AGCTTTCTCA 
GGCGTGCCCA GCCCTACCGG GGCAGAGGGG GAAGCTGGGC AGAGCCAAGA GGAGAAATCC 
AGCCCAGCAT CCCAAGCTGG CTCTCATGCT ACGGCCCCCA CCTCCGGCAG TACGGTTCTA 
GAAAGAGGAA CTGCAGGGTC CTTGTCCCCT GGCGTTGTGG AAGCTAATGC CCCCATGGTC 
CAACCTAGTA CATCACCGGG CCCCGCACAG GAAGAGGCAG GAGAGAACCG CGTGGATGGT 
GAGAGAAATG TGAAGAGCTG CGCGTCTCCA CGGGGCGGAG AAGCTGGGTT GCCGGAACCC 
AACACAGCTG GTCCGTCTTC AGACACGGCA GACGTTTCTA AAGAAGGAGG GAACGATGAG 
GTGCTGACCC CTACCAGACC CAGGACAACG GAAGACCTGT TTGCAGCCAT TCACAGATCC 
AAAAGGAAAG TCCTTGGTCG GAAGGACTCC GAAGATGATC ACACTCGAAA TCATTCTCCC 
TCCCCGCCTG TGACACCCAC AGGTGCTGCC CCTAGCCTGG CCTCTCCAAA GCAAGTAGGG 
TCCATCCAGC GAAGCCTAAA AAAGAGCAGT ACCAGCAGCG ACAACTTCAA AGCTCTGCTG 
CTCAAGAAGG GGAGTCGCTC GGACACCAGC GCTCGCATGT CCGCGGCGGA GATGCTCAAG 
TCCACGGACC CTCGATTCCA GAGATCTAGG TCGGAGCCTT CGCCAGAGGT CCCGGACAGC 
CCATCCAGCT TCTCTCCCAA TAAGAACAGG AGAGCTCCGG AGGAGTGGGC CAAGAATGAA 
GGACTGATGC CTCGGAGTCT GTCCTTTTCC GGCCCTCGGT ACAGCCGCAG CAGGACGCCA 
CCCTCTGCGG CCAGCAGCAG GTACAGCATG AGGAACCGCA TCCAAAGCAG CCCCATGACA 
GTCATCTCTG AGGGAGAGGG AGAGCCTGCC GAGCCCGCAG ACAACAAAGT ACGCAGGACC 
CTGGACACTA CGAGGGGATG CTCTCTGGAC AGACTGGCGG GACAGGAGAC GGACCGGGGC 
AGCCCGCTGT GTAGCGAGGA GCCTGCCTCT GTGGATGGGA TAGGAAGGGC TAAGGACGAC 
GGTCCCTCGG AGCAGTGTAG GGGTCCTGAA CAGAAGAGTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nhsl1 ( XM_002725450.5 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_002725496.1 Rattus norvegicus NHS-like 1 (Nhsl1), transcript variant X4, mRNA.

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CloneID	ORa13977
Clone ID Related Accession (Same CDS sequence)	XM_002728626.5 , XM_002725450.5
Accession Version	XM_002725450.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4782bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	NHS-like protein 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (AC_000069.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_002725450.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAAAGG AAGGCTCTGC AGGTTCCTTC AGAATCAAGG CAAACCCAGG CTCCCTCTCA 
CGTGCCGTGA GTTGGATAAA TTTCTCCTCC CTGTCCAGGC AGACAAAGAG GCTGTTTCGC 
AGCGATGGAG AGCTGTCCGT TTGTGGGCAG CAGGTGGAGG CTGATGATGA GAATTGGATA 
TACAGGACCC AGCCCAGAAA AGCGGTTTCC AACTTAGACG AGGAGAGCCG ATGGACCGTC 
CACTATACGG CGCCGTGGCA CCAGCAGGAG AATGTGTTCC TCCCCTCCAC AAGACCCCCC 
TGTGTTGAGG ACCTGCACCG CCAAGCGAAG CTCAACCTCA AATCAGTGCT GAGGGAATGT 
GACAAGCTGA GGCGCGATGG CTGCCGAAGT TCTCAGTACT ATTCTCAGGG ACCCACCTTT 
GCAGCCAGCT CCAGCCCAGG CGATGACTAT CAAGAGGAAG ATGCAGAAAC AGACCGAAAG 
TATTCTCTCT CTTCATCTGA AGAAGAAAGA TTTATTGGTA TCAGGAGACC TAAAACGCCA 
ACCTCAGGTG ACTTCTCTGA CCTTCATACT CAGACAAACT GGACCAAGTC CCTGCCGCTG 
CCAACACCAG AAGAGAAGAC ACGACAGCAA GCCCAGACAG TCCAGGCTGA TGTGGTTCCT 
ATTAACATAA CTGCGTCAGC CACGGGCCAA GAGGATGATG GTAGCACCCA TTCTCTATAC 
TCCCCAGACC ACTACTCTAC ATTAGGAAGG CTTGATAGCT ATCGGTCCAC GGGGCAATGC 
TTGGAAACCA GGGACTCTGG CTGTCAGACA GAAGAAGTGA AAGTCATCCC ACCGTCAGTG 
AGAAGGATCA GGGCTCACAA GGGGCTGGGC ATTGCTGCCC AGATGAGCCA CCTCTCGGGT 
TCCTCTGGCA ACATGTCTGT GCTGAGCGAC TCGGCTGGTA TCGTGTTCCC ATCTCGCCTC 
AATAACGATA CTGGTTTCCA CAGCCTTCCA CGCGCTGGCG CAAGGGCAAG CACGTATTCC 
CTGGAGGCAA GGATGGGTGC CCTGGGCTCT GCTGAAGATA CGGATGATAC TTTTCCCTAC 
CGAGGGGGTA GCTCCCAGGG GCCTGAAAAC TTTGCACATT TAGGGGGTGC CTCGAGTACC 
GGGATGCTTT CGAGGCCCAA ATCCCAGCAG CTGAGGCTCT TGGAGAGCCC AGCGTGTGTG 
GTTTCACCGC ACGCGGCCTA CTCTACCAGC GTCATCCCAA ACGCCACACT GCTGTCCTCT 
TCGGAGGTTA TTGTCATCCA CACCGCTCAG AGTGCAGGAC AGCTGGACAG TAGAGTCCCC 
GGCTCATCCT CATACTCAAA GATAAAACCC AGAGACCATC CCACACCCAG GTGCTCTGCG 
AGAGATGAGC GCCAGTCCCC ACGACATCAC TGGAACGAGG GTCATATCAT TCACTCACAG 
GCTTTAGCCT CCGCTGCCCC CGGTGCCACC GCACTGTTAT CCCTCCGTGA CTCAGAGGTC 
TCTCTCAATG CCCCAGCTAT CAATAGGGAG AACGGGTCCC AAGCCATACT TTATCACTGT 
AGAAGCAACC TGGCCTTTCC TGCCCACCCT CCGGATGTGG ATGGCAAGAG CGAGTGCAGT 
TCTTCAGGGG ACAGGGGATG TGGCAGTTCT GAGTCCTGGG AATACAAAGC CTCCAGCAAT 
GGGCGGGCTT CCCCACTGAA GCCGCACCTT GCGACTCCCG GTTGCTCTAC CCCCACAAGT 
AACATGAGCA GCTGCAGCTT GGACCAAGCA TCCCTCAAGG AGGATGCCAG GTCCCTGTAT 
TCAGAGGACC ACGATGGTTA CTATATGACC GCCCAGGGTG ATTCTAGACA CGAGGCTGGG 
AGCGCGTACG GTATCAGTGA TGGCTTTGGA AACCCCAGGC ACAGCACGGT CAATGTTTTT 
GACGGAAGGG CTCAGAGAAG CCAAGGGGAT CAGGCCACTC AGCAGGATAG AATCCTCTCA 
AGAAACATCT CTCTGAAGAA AGCAAAAAAA CCACCACCGC CACCATCCCG GACAGATTCT 
CTGCGAAGGA TTCCCAAGAA GAACAACCAG ACAAATGGGC AGGTGCTCAA CGAGAGCCTG 
ATCGCCTCAC TCCAGCATTC TCTGCAGCTA AGCCTCCCTG GCCAAGGCAG CGGTTCCCCT 
TCCCAGAGCC CCTGCAGTGA CTGTGAGGAA CCCTGGCTCC CCAGGTCCAG AAGCCAGAGC 
ATTGTTAGCG AGGGTAGTAG CTTGACCTCC ACCACCACTC CCAACGTGTA TTCTCTGTGC 
GGGCTCACTC CATCTCAGAG CGACACAAGC AGCGTCAAGT CGGAATACGC AGACCCCTGG 
GGCTACTACA TTGACTACAC AACTTTGCAG GGAGATCCAG GGAACCCCAC AGGGGGCTGC 
CCAGCCAGTA GTGAGGCAGC CACTGGAAAT GGGCCAGTGC GCCACATCCA GGAGGGGTCG 
AGAGCCCCAG TGCCCCAAGT GCCGGGCTGC TCCGTGAAAC CAAAGATCAC CTCACCGGAG 
AAGTCACAGA GAGTCACCTC TCCATCCAGT GGGTATTCTA GCCAGTCGAA TACACCCACA 
GCACTCACCC CCGTGCCTGT GTTTTTAAAA TCGATGTCAC CAGCAAATGG GAAGGGGAAG 
GCCAAGCCCA AGGTACCAGA AAGAAAATCG TCTCTGATCT CTTCAGTGTC CATCTCCTCA 
TCGTCCACTT CCCTCTCCTC TAATACCTCT ACGGAAGGAA GTGGGACCGT GAAGAAACTG 
GATTCAGCCC TGGCCTCGGC CCTTGCTCCT CCCCCTCCTC CTCTTCCCGC GCTGCCTTCT 
CCGAGTCTGG CAAACAAGTC CCCCTTCCTT CCACCTCCTC CTCCTCTAGC AGATTGCTCC 
GAGGGCTCTC CCTTACCGCC CTCTCCTGTG TTCCCCCCTC CACCACCAGA AGCCCTTGTT 
CCCTTCTGCT CCCCCACTGA CAGGTGCCTT TCTCCTTCCC CCACAGCTTT TAGCCCCCCT 
CTTCCAAGAT GCTCACCTCC CTTGCCAGCA CCTCCACCTT TCTTGCCCCC ATCAGAACCC 
CCACCTGCTC CGCCTCTGGA CCCCAAATTC ATGAAAGAAA ACAGGCCCTT TATCAAAAAC 
TCTAGCCAGT CCGAATCCTC TAGGGAGGCC CTCAGGCGGC CTGCCAACAA GGAGGAGGGC 
TGCCGACCCT CGATGCCCCT GATCACCACA GAAGCTTTGC AGATGGTGCA GTTGAGGCCA 
GTGAGGAAGA ACTCAGGTGC TGAGACGGTC CTGTTTTCTG AACCATCAGC TCAGGAACCA 
CGAACCCCGA CTGCTCCACA GTATCACTTA AAGCCATCTG CTTTCCTCAA ATCCCGAAAT 
AGCATAAATG AAATGGAGAG TGAAAGCCAG GCTGCCTCTG TGACGAGCTC GCTTCCGATG 
CCTGCCAAGA GCCAGAGTCA GGGTGACCAT GACAGTGCAG TCGAGCGTGG CGGCCTTCAG 
AGCTGCAGCG ATGGAGCTCC AGGCCCGGGT CCCAGCCTCA GGACCGCTCT GCTCCCAGAC 
TCTTCACCCA GCAGGAAGCC ACCCCCCATC TCCAAGAAGC CCAAACTGTT CCTGGTGGTA 
CCACCTCCGC AGAGAGATTT CACAGCGGAG CCCACAGAGA ACGGGAGCGA AGCTTTCTCA 
GGCGTGCCCA GCCCTACCGG GGCAGAGGGG GAAGCTGGGC AGAGCCAAGA GGAGAAATCC 
AGCCCAGCAT CCCAAGCTGG CTCTCATGCT ACGGCCCCCA CCTCCGGCAG TACGGTTCTA 
GAAAGAGGAA CTGCAGGGTC CTTGTCCCCT GGCGTTGTGG AAGCTAATGC CCCCATGGTC 
CAACCTAGTA CATCACCGGG CCCCGCACAG GAAGAGGCAG GAGAGAACCG CGTGGATGGT 
GAGAGAAATG TGAAGAGCTG CGCGTCTCCA CGGGGCGGAG AAGCTGGGTT GCCGGAACCC 
AACACAGCTG GTCCGTCTTC AGACACGGCA GACGTTTCTA AAGAAGGAGG GAACGATGAG 
GTGCTGACCC CTACCAGACC CAGGACAACG GAAGACCTGT TTGCAGCCAT TCACAGATCC 
AAAAGGAAAG TCCTTGGTCG GAAGGACTCC GAAGATGATC ACACTCGAAA TCATTCTCCC 
TCCCCGCCTG TGACACCCAC AGGTGCTGCC CCTAGCCTGG CCTCTCCAAA GCAAGTAGGG 
TCCATCCAGC GAAGCCTAAA AAAGAGCAGT ACCAGCAGCG ACAACTTCAA AGCTCTGCTG 
CTCAAGAAGG GGAGTCGCTC GGACACCAGC GCTCGCATGT CCGCGGCGGA GATGCTCAAG 
TCCACGGACC CTCGATTCCA GAGATCTAGG TCGGAGCCTT CGCCAGAGGT CCCGGACAGC 
CCATCCAGCT TCTCTCCCAA TAAGAACAGG AGAGCTCCGG AGGAGTGGGC CAAGAATGAA 
GGACTGATGC CTCGGAGTCT GTCCTTTTCC GGCCCTCGGT ACAGCCGCAG CAGGACGCCA 
CCCTCTGCGG CCAGCAGCAG GTACAGCATG AGGAACCGCA TCCAAAGCAG CCCCATGACA 
GTCATCTCTG AGGGAGAGGG AGAGCCTGCC GAGCCCGCAG ACAACAAAGT ACGCAGGACC 
CTGGACACTA CGAGGGGATG CTCTCTGGAC AGACTGGCGG GACAGGAGAC GGACCGGGGC 
AGCCCGCTGT GTAGCGAGGA GCCTGCCTCT GTGGATGGGA TAGGAAGGGC TAAGGACGAC 
GGTCCCTCGG AGCAGTGTAG GGGTCCTGAA CAGAAGAGTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002725496.1
CDS	2861..7642
Translation	MKKEGSAGSFRIKANPGSLSRAVSWINFSSLSRQTKRLFRSDGELSVCGQQVEADDENWIYRTQPRKAVSNLDEESRWTVHYTAPWHQQENVFLPSTRPPCVEDLHRQAKLNLKSVLRECDKLRRDGCRSSQYYSQGPTFAASSSPGDDYQEEDAETDRKYSLSSSEEERFIGIRRPKTPTSGDFSDLHTQTNWTKSLPLPTPEEKTRQQAQTVQADVVPINITASATGQEDDGSTHSLYSPDHYSTLGRLDSYRSTGQCLETRDSGCQTEEVKVIPPSVRRIRAHKGLGIAAQMSHLSGSSGNMSVLSDSAGIVFPSRLNNDTGFHSLPRAGARASTYSLEARMGALGSAEDTDDTFPYRGGSSQGPENFAHLGGASSTGMLSRPKSQQLRLLESPACVVSPHAAYSTSVIPNATLLSSSEVIVIHTAQSAGQLDSRVPGSSSYSKIKPRDHPTPRCSARDERQSPRHHWNEGHIIHSQALASAAPGATALLSLRDSEVSLNAPAINRENGSQAILYHCRSNLAFPAHPPDVDGKSECSSSGDRGCGSSESWEYKASSNGRASPLKPHLATPGCSTPTSNMSSCSLDQASLKEDARSLYSEDHDGYYMTAQGDSRHEAGSAYGISDGFGNPRHSTVNVFDGRAQRSQGDQATQQDRILSRNISLKKAKKPPPPPSRTDSLRRIPKKNNQTNGQVLNESLIASLQHSLQLSLPGQGSGSPSQSPCSDCEEPWLPRSRSQSIVSEGSSLTSTTTPNVYSLCGLTPSQSDTSSVKSEYADPWGYYIDYTTLQGDPGNPTGGCPASSEAATGNGPVRHIQEGSRAPVPQVPGCSVKPKITSPEKSQRVTSPSSGYSSQSNTPTALTPVPVFLKSMSPANGKGKAKPKVPERKSSLISSVSISSSSTSLSSNTSTEGSGTVKKLDSALASALAPPPPPLPALPSPSLANKSPFLPPPPPLADCSEGSPLPPSPVFPPPPPEALVPFCSPTDRCLSPSPTAFSPPLPRCSPPLPAPPPFLPPSEPPPAPPLDPKFMKENRPFIKNSSQSESSREALRRPANKEEGCRPSMPLITTEALQMVQLRPVRKNSGAETVLFSEPSAQEPRTPTAPQYHLKPSAFLKSRNSINEMESESQAASVTSSLPMPAKSQSQGDHDSAVERGGLQSCSDGAPGPGPSLRTALLPDSSPSRKPPPISKKPKLFLVVPPPQRDFTAEPTENGSEAFSGVPSPTGAEGEAGQSQEEKSSPASQAGSHATAPTSGSTVLERGTAGSLSPGVVEANAPMVQPSTSPGPAQEEAGENRVDGERNVKSCASPRGGEAGLPEPNTAGPSSDTADVSKEGGNDEVLTPTRPRTTEDLFAAIHRSKRKVLGRKDSEDDHTRNHSPSPPVTPTGAAPSLASPKQVGSIQRSLKKSSTSSDNFKALLLKKGSRSDTSARMSAAEMLKSTDPRFQRSRSEPSPEVPDSPSSFSPNKNRRAPEEWAKNEGLMPRSLSFSGPRYSRSRTPPSAASSRYSMRNRIQSSPMTVISEGEGEPAEPADNKVRRTLDTTRGCSLDRLAGQETDRGSPLCSEEPASVDGIGRAKDDGPSEQCRGPEQKS

Target ORF information:

RefSeq Version	XM_002725450.5
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus NHS-like 1 (Nhsl1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002725450.5

ORF Insert Sequence:

ATGAAAAAGG AAGGCTCTGC AGGTTCCTTC AGAATCAAGG CAAACCCAGG CTCCCTCTCA 
CGTGCCGTGA GTTGGATAAA TTTCTCCTCC CTGTCCAGGC AGACAAAGAG GCTGTTTCGC 
AGCGATGGAG AGCTGTCCGT TTGTGGGCAG CAGGTGGAGG CTGATGATGA GAATTGGATA 
TACAGGACCC AGCCCAGAAA AGCGGTTTCC AACTTAGACG AGGAGAGCCG ATGGACCGTC 
CACTATACGG CGCCGTGGCA CCAGCAGGAG AATGTGTTCC TCCCCTCCAC AAGACCCCCC 
TGTGTTGAGG ACCTGCACCG CCAAGCGAAG CTCAACCTCA AATCAGTGCT GAGGGAATGT 
GACAAGCTGA GGCGCGATGG CTGCCGAAGT TCTCAGTACT ATTCTCAGGG ACCCACCTTT 
GCAGCCAGCT CCAGCCCAGG CGATGACTAT CAAGAGGAAG ATGCAGAAAC AGACCGAAAG 
TATTCTCTCT CTTCATCTGA AGAAGAAAGA TTTATTGGTA TCAGGAGACC TAAAACGCCA 
ACCTCAGGTG ACTTCTCTGA CCTTCATACT CAGACAAACT GGACCAAGTC CCTGCCGCTG 
CCAACACCAG AAGAGAAGAC ACGACAGCAA GCCCAGACAG TCCAGGCTGA TGTGGTTCCT 
ATTAACATAA CTGCGTCAGC CACGGGCCAA GAGGATGATG GTAGCACCCA TTCTCTATAC 
TCCCCAGACC ACTACTCTAC ATTAGGAAGG CTTGATAGCT ATCGGTCCAC GGGGCAATGC 
TTGGAAACCA GGGACTCTGG CTGTCAGACA GAAGAAGTGA AAGTCATCCC ACCGTCAGTG 
AGAAGGATCA GGGCTCACAA GGGGCTGGGC ATTGCTGCCC AGATGAGCCA CCTCTCGGGT 
TCCTCTGGCA ACATGTCTGT GCTGAGCGAC TCGGCTGGTA TCGTGTTCCC ATCTCGCCTC 
AATAACGATA CTGGTTTCCA CAGCCTTCCA CGCGCTGGCG CAAGGGCAAG CACGTATTCC 
CTGGAGGCAA GGATGGGTGC CCTGGGCTCT GCTGAAGATA CGGATGATAC TTTTCCCTAC 
CGAGGGGGTA GCTCCCAGGG GCCTGAAAAC TTTGCACATT TAGGGGGTGC CTCGAGTACC 
GGGATGCTTT CGAGGCCCAA ATCCCAGCAG CTGAGGCTCT TGGAGAGCCC AGCGTGTGTG 
GTTTCACCGC ACGCGGCCTA CTCTACCAGC GTCATCCCAA ACGCCACACT GCTGTCCTCT 
TCGGAGGTTA TTGTCATCCA CACCGCTCAG AGTGCAGGAC AGCTGGACAG TAGAGTCCCC 
GGCTCATCCT CATACTCAAA GATAAAACCC AGAGACCATC CCACACCCAG GTGCTCTGCG 
AGAGATGAGC GCCAGTCCCC ACGACATCAC TGGAACGAGG GTCATATCAT TCACTCACAG 
GCTTTAGCCT CCGCTGCCCC CGGTGCCACC GCACTGTTAT CCCTCCGTGA CTCAGAGGTC 
TCTCTCAATG CCCCAGCTAT CAATAGGGAG AACGGGTCCC AAGCCATACT TTATCACTGT 
AGAAGCAACC TGGCCTTTCC TGCCCACCCT CCGGATGTGG ATGGCAAGAG CGAGTGCAGT 
TCTTCAGGGG ACAGGGGATG TGGCAGTTCT GAGTCCTGGG AATACAAAGC CTCCAGCAAT 
GGGCGGGCTT CCCCACTGAA GCCGCACCTT GCGACTCCCG GTTGCTCTAC CCCCACAAGT 
AACATGAGCA GCTGCAGCTT GGACCAAGCA TCCCTCAAGG AGGATGCCAG GTCCCTGTAT 
TCAGAGGACC ACGATGGTTA CTATATGACC GCCCAGGGTG ATTCTAGACA CGAGGCTGGG 
AGCGCGTACG GTATCAGTGA TGGCTTTGGA AACCCCAGGC ACAGCACGGT CAATGTTTTT 
GACGGAAGGG CTCAGAGAAG CCAAGGGGAT CAGGCCACTC AGCAGGATAG AATCCTCTCA 
AGAAACATCT CTCTGAAGAA AGCAAAAAAA CCACCACCGC CACCATCCCG GACAGATTCT 
CTGCGAAGGA TTCCCAAGAA GAACAACCAG ACAAATGGGC AGGTGCTCAA CGAGAGCCTG 
ATCGCCTCAC TCCAGCATTC TCTGCAGCTA AGCCTCCCTG GCCAAGGCAG CGGTTCCCCT 
TCCCAGAGCC CCTGCAGTGA CTGTGAGGAA CCCTGGCTCC CCAGGTCCAG AAGCCAGAGC 
ATTGTTAGCG AGGGTAGTAG CTTGACCTCC ACCACCACTC CCAACGTGTA TTCTCTGTGC 
GGGCTCACTC CATCTCAGAG CGACACAAGC AGCGTCAAGT CGGAATACGC AGACCCCTGG 
GGCTACTACA TTGACTACAC AACTTTGCAG GGAGATCCAG GGAACCCCAC AGGGGGCTGC 
CCAGCCAGTA GTGAGGCAGC CACTGGAAAT GGGCCAGTGC GCCACATCCA GGAGGGGTCG 
AGAGCCCCAG TGCCCCAAGT GCCGGGCTGC TCCGTGAAAC CAAAGATCAC CTCACCGGAG 
AAGTCACAGA GAGTCACCTC TCCATCCAGT GGGTATTCTA GCCAGTCGAA TACACCCACA 
GCACTCACCC CCGTGCCTGT GTTTTTAAAA TCGATGTCAC CAGCAAATGG GAAGGGGAAG 
GCCAAGCCCA AGGTACCAGA AAGAAAATCG TCTCTGATCT CTTCAGTGTC CATCTCCTCA 
TCGTCCACTT CCCTCTCCTC TAATACCTCT ACGGAAGGAA GTGGGACCGT GAAGAAACTG 
GATTCAGCCC TGGCCTCGGC CCTTGCTCCT CCCCCTCCTC CTCTTCCCGC GCTGCCTTCT 
CCGAGTCTGG CAAACAAGTC CCCCTTCCTT CCACCTCCTC CTCCTCTAGC AGATTGCTCC 
GAGGGCTCTC CCTTACCGCC CTCTCCTGTG TTCCCCCCTC CACCACCAGA AGCCCTTGTT 
CCCTTCTGCT CCCCCACTGA CAGGTGCCTT TCTCCTTCCC CCACAGCTTT TAGCCCCCCT 
CTTCCAAGAT GCTCACCTCC CTTGCCAGCA CCTCCACCTT TCTTGCCCCC ATCAGAACCC 
CCACCTGCTC CGCCTCTGGA CCCCAAATTC ATGAAAGAAA ACAGGCCCTT TATCAAAAAC 
TCTAGCCAGT CCGAATCCTC TAGGGAGGCC CTCAGGCGGC CTGCCAACAA GGAGGAGGGC 
TGCCGACCCT CGATGCCCCT GATCACCACA GAAGCTTTGC AGATGGTGCA GTTGAGGCCA 
GTGAGGAAGA ACTCAGGTGC TGAGACGGTC CTGTTTTCTG AACCATCAGC TCAGGAACCA 
CGAACCCCGA CTGCTCCACA GTATCACTTA AAGCCATCTG CTTTCCTCAA ATCCCGAAAT 
AGCATAAATG AAATGGAGAG TGAAAGCCAG GCTGCCTCTG TGACGAGCTC GCTTCCGATG 
CCTGCCAAGA GCCAGAGTCA GGGTGACCAT GACAGTGCAG TCGAGCGTGG CGGCCTTCAG 
AGCTGCAGCG ATGGAGCTCC AGGCCCGGGT CCCAGCCTCA GGACCGCTCT GCTCCCAGAC 
TCTTCACCCA GCAGGAAGCC ACCCCCCATC TCCAAGAAGC CCAAACTGTT CCTGGTGGTA 
CCACCTCCGC AGAGAGATTT CACAGCGGAG CCCACAGAGA ACGGGAGCGA AGCTTTCTCA 
GGCGTGCCCA GCCCTACCGG GGCAGAGGGG GAAGCTGGGC AGAGCCAAGA GGAGAAATCC 
AGCCCAGCAT CCCAAGCTGG CTCTCATGCT ACGGCCCCCA CCTCCGGCAG TACGGTTCTA 
GAAAGAGGAA CTGCAGGGTC CTTGTCCCCT GGCGTTGTGG AAGCTAATGC CCCCATGGTC 
CAACCTAGTA CATCACCGGG CCCCGCACAG GAAGAGGCAG GAGAGAACCG CGTGGATGGT 
GAGAGAAATG TGAAGAGCTG CGCGTCTCCA CGGGGCGGAG AAGCTGGGTT GCCGGAACCC 
AACACAGCTG GTCCGTCTTC AGACACGGCA GACGTTTCTA AAGAAGGAGG GAACGATGAG 
GTGCTGACCC CTACCAGACC CAGGACAACG GAAGACCTGT TTGCAGCCAT TCACAGATCC 
AAAAGGAAAG TCCTTGGTCG GAAGGACTCC GAAGATGATC ACACTCGAAA TCATTCTCCC 
TCCCCGCCTG TGACACCCAC AGGTGCTGCC CCTAGCCTGG CCTCTCCAAA GCAAGTAGGG 
TCCATCCAGC GAAGCCTAAA AAAGAGCAGT ACCAGCAGCG ACAACTTCAA AGCTCTGCTG 
CTCAAGAAGG GGAGTCGCTC GGACACCAGC GCTCGCATGT CCGCGGCGGA GATGCTCAAG 
TCCACGGACC CTCGATTCCA GAGATCTAGG TCGGAGCCTT CGCCAGAGGT CCCGGACAGC 
CCATCCAGCT TCTCTCCCAA TAAGAACAGG AGAGCTCCGG AGGAGTGGGC CAAGAATGAA 
GGACTGATGC CTCGGAGTCT GTCCTTTTCC GGCCCTCGGT ACAGCCGCAG CAGGACGCCA 
CCCTCTGCGG CCAGCAGCAG GTACAGCATG AGGAACCGCA TCCAAAGCAG CCCCATGACA 
GTCATCTCTG AGGGAGAGGG AGAGCCTGCC GAGCCCGCAG ACAACAAAGT ACGCAGGACC 
CTGGACACTA CGAGGGGATG CTCTCTGGAC AGACTGGCGG GACAGGAGAC GGACCGGGGC 
AGCCCGCTGT GTAGCGAGGA GCCTGCCTCT GTGGATGGGA TAGGAAGGGC TAAGGACGAC 
GGTCCCTCGG AGCAGTGTAG GGGTCCTGAA CAGAAGAGTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nhsl1 ( XM_002728625.5 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_002728671.1 Rattus norvegicus NHS-like 1 (Nhsl1), transcript variant X5, mRNA.

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CloneID	ORa13978
Clone ID Related Accession (Same CDS sequence)	XM_002728625.5 , XM_002725449.5
Accession Version	XM_002728625.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4770bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	NHS-like protein 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_002728625.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGTCT TCGTTAATGC GAAGATTAAA TCTTTCTTTA AACTTTTTAA GAAGAAAACG 
GTTTCCAACT TAGACGAGGA GAGCCGATGG ACCGTCCACT ATACGGCGCC GTGGCACCAG 
CAGGAGAATG TGTTCCTCCC CTCCACAAGA CCCCCCTGTG TTGAGGACCT GCACCGCCAA 
GCGAAGCTCA ACCTCAAATC AGTGCTGAGG GAATGTGACA AGCTGAGGCG CGATGGCTGC 
CGAAGTTCTC AGTACTATTC TCAGGGACCC ACCTTTGCAG CCAGCTCCAG CCCAGGCGAT 
GACTATCAAG AGGAAGATGC AGAAACAGAC CGAAAGTATT CTCTCTCTTC ATCTGAAGAA 
GAAAGATTTA TTGGTATCAG GAGACCTAAA ACGCCAACCT CAGGTGACTT CTCTGACCTT 
CATACTCAGA CAAACTGGAC CAAGTCCCTG CCGCTGCCAA CACCAGAAGA GAAGACACGA 
CAGCAAGCCC AGACAGTCCA GGCTGATGTG GTTCCTATTA ACATAACTGG GGAGAATTTT 
GATCGTCAGG CCAGCCTTCG GCGGTCTCTA ATTTACACTG ACACTCTGGT AAGACGACCG 
AAGAAAGTCA AAAGGAGAAA GACTATTTCA GGAATCCCTG ACATCATTCA GAAGGAGCTA 
GCGTCAGCCA CGGGCCAAGA GGATGATGGT AGCACCCATT CTCTATACTC CCCAGACCAC 
TACTCTACAT TAGGAAGGCT TGATAGCTAT CGGTCCACGG GGCAATGCTT GGAAACCAGG 
GACTCTGGCT GTCAGACAGA AGAAGTGAAA GTCATCCCAC CGTCAGTGAG AAGGATCAGG 
GCTCACAAGG GGCTGGGCAT TGCTGCCCAG ATGAGCCACC TCTCGGGTTC CTCTGGCAAC 
ATGTCTGTGC TGAGCGACTC GGCTGGTATC GTGTTCCCAT CTCGCCTCAA TAACGATACT 
GGTTTCCACA GCCTTCCACG CGCTGGCGCA AGGGCAAGCA CGTATTCCCT GGAGGCAAGG 
ATGGGTGCCC TGGGCTCTGC TGAAGATACG GATGATACTT TTCCCTACCG AGGGGGTAGC 
TCCCAGGGGC CTGAAAACTT TGCACATTTA GGGGGTGCCT CGAGTACCGG GATGCTTTCG 
AGGCCCAAAT CCCAGCAGCT GAGGCTCTTG GAGAGCCCAG CGTGTGTGGT TTCACCGCAC 
GCGGCCTACT CTACCAGCGT CATCCCAAAC GCCACACTGC TGTCCTCTTC GGAGGTTATT 
GTCATCCACA CCGCTCAGAG TGCAGGACAG CTGGACAGTA GAGTCCCCGG CTCATCCTCA 
TACTCAAAGA TAAAACCCAG AGACCATCCC ACACCCAGGT GCTCTGCGAG AGATGAGCGC 
CAGTCCCCAC GACATCACTG GAACGAGGGT CATATCATTC ACTCACAGGC TTTAGCCTCC 
GCTGCCCCCG GTGCCACCGC ACTGTTATCC CTCCGTGACT CAGAGGTCTC TCTCAATGCC 
CCAGCTATCA ATAGGGAGAA CGGGTCCCAA GCCATACTTT ATCACTGTAG AAGCAACCTG 
GCCTTTCCTG CCCACCCTCC GGATGTGGAT GGCAAGAGCG AGTGCAGTTC TTCAGGGGAC 
AGGGGATGTG GCAGTTCTGA GTCCTGGGAA TACAAAGCCT CCAGCAATGG GCGGGCTTCC 
CCACTGAAGC CGCACCTTGC GACTCCCGGT TGCTCTACCC CCACAAGTAA CATGAGCAGC 
TGCAGCTTGG ACCAAGCATC CCTCAAGGAG GATGCCAGGT CCCTGTATTC AGAGGACCAC 
GATGGTTACT ATATGACCGC CCAGGGTGAT TCTAGACACG AGGCTGGGAG CGCGTACGGT 
ATCAGTGATG GCTTTGGAAA CCCCAGGCAC AGCACGGTCA ATGTTTTTGA CGGAAGGGCT 
CAGAGAAGCC AAGGGGATCA GGCCACTCAG CAGGATAGAA TCCTCTCAAG AAACATCTCT 
CTGAAGAAAG CAAAAAAACC ACCACCGCCA CCATCCCGGA CAGATTCTCT GCGAAGGATT 
CCCAAGAAGA ACAACCAGAC AAATGGGCAG GTGCTCAACG AGAGCCTGAT CGCCTCACTC 
CAGCATTCTC TGCAGCTAAG CCTCCCTGGC CAAGGCAGCG GTTCCCCTTC CCAGAGCCCC 
TGCAGTGACT GTGAGGAACC CTGGCTCCCC AGGTCCAGAA GCCAGAGCAT TGTTAGCGAG 
GGTAGTAGCT TGACCTCCAC CACCACTCCC AACGTGTATT CTCTGTGCGG GCTCACTCCA 
TCTCAGAGCG ACACAAGCAG CGTCAAGTCG GAATACGCAG ACCCCTGGGG CTACTACATT 
GACTACACAA CTTTGCAGGG AGATCCAGGG AACCCCACAG GGGGCTGCCC AGCCAGTAGT 
GAGGCAGCCA CTGGAAATGG GCCAGTGCGC CACATCCAGG AGGGGTCGAG AGCCCCAGTG 
CCCCAAGTGC CGGGCTGCTC CGTGAAACCA AAGATCACCT CACCGGAGAA GTCACAGAGA 
GTCACCTCTC CATCCAGTGG GTATTCTAGC CAGTCGAATA CACCCACAGC ACTCACCCCC 
GTGCCTGTGT TTTTAAAATC GATGTCACCA GCAAATGGGA AGGGGAAGGC CAAGCCCAAG 
GTACCAGAAA GAAAATCGTC TCTGATCTCT TCAGTGTCCA TCTCCTCATC GTCCACTTCC 
CTCTCCTCTA ATACCTCTAC GGAAGGAAGT GGGACCGTGA AGAAACTGGA TTCAGCCCTG 
GCCTCGGCCC TTGCTCCTCC CCCTCCTCCT CTTCCCGCGC TGCCTTCTCC GAGTCTGGCA 
AACAAGTCCC CCTTCCTTCC ACCTCCTCCT CCTCTAGCAG ATTGCTCCGA GGGCTCTCCC 
TTACCGCCCT CTCCTGTGTT CCCCCCTCCA CCACCAGAAG CCCTTGTTCC CTTCTGCTCC 
CCCACTGACA GGTGCCTTTC TCCTTCCCCC ACAGCTTTTA GCCCCCCTCT TCCAAGATGC 
TCACCTCCCT TGCCAGCACC TCCACCTTTC TTGCCCCCAT CAGAACCCCC ACCTGCTCCG 
CCTCTGGACC CCAAATTCAT GAAAGAAAAC AGGCCCTTTA TCAAAAACTC TAGCCAGTCC 
GAATCCTCTA GGGAGGCCCT CAGGCGGCCT GCCAACAAGG AGGAGGGCTG CCGACCCTCG 
ATGCCCCTGA TCACCACAGA AGCTTTGCAG ATGGTGCAGT TGAGGCCAGT GAGGAAGAAC 
TCAGGTGCTG AGACGGTCCT GTTTTCTGAA CCATCAGCTC AGGAACCACG AACCCCGACT 
GCTCCACAGT ATCACTTAAA GCCATCTGCT TTCCTCAAAT CCCGAAATAG CATAAATGAA 
ATGGAGAGTG AAAGCCAGGC TGCCTCTGTG ACGAGCTCGC TTCCGATGCC TGCCAAGAGC 
CAGAGTCAGG GTGACCATGA CAGTGCAGTC GAGCGTGGCG GCCTTCAGAG CTGCAGCGAT 
GGAGCTCCAG GCCCGGGTCC CAGCCTCAGG ACCGCTCTGC TCCCAGACTC TTCACCCAGC 
AGGAAGCCAC CCCCCATCTC CAAGAAGCCC AAACTGTTCC TGGTGGTACC ACCTCCGCAG 
AGAGATTTCA CAGCGGAGCC CACAGAGAAC GGGAGCGAAG CTTTCTCAGG CGTGCCCAGC 
CCTACCGGGG CAGAGGGGGA AGCTGGGCAG AGCCAAGAGG AGAAATCCAG CCCAGCATCC 
CAAGCTGGCT CTCATGCTAC GGCCCCCACC TCCGGCAGTA CGGTTCTAGA AAGAGGAACT 
GCAGGGTCCT TGTCCCCTGG CGTTGTGGAA GCTAATGCCC CCATGGTCCA ACCTAGTACA 
TCACCGGGCC CCGCACAGGA AGAGGCAGGA GAGAACCGCG TGGATGGTGA GAGAAATGTG 
AAGAGCTGCG CGTCTCCACG GGGCGGAGAA GCTGGGTTGC CGGAACCCAA CACAGCTGGT 
CCGTCTTCAG ACACGGCAGA CGTTTCTAAA GAAGGAGGGA ACGATGAGGT GCTGACCCCT 
ACCAGACCCA GGACAACGGA AGACCTGTTT GCAGCCATTC ACAGATCCAA AAGGAAAGTC 
CTTGGTCGGA AGGACTCCGA AGATGATCAC ACTCGAAATC ATTCTCCCTC CCCGCCTGTG 
ACACCCACAG GTGCTGCCCC TAGCCTGGCC TCTCCAAAGC AAGTAGGGTC CATCCAGCGA 
AGCCTAAAAA AGAGCAGTAC CAGCAGCGAC AACTTCAAAG CTCTGCTGCT CAAGAAGGGG 
AGTCGCTCGG ACACCAGCGC TCGCATGTCC GCGGCGGAGA TGCTCAAGTC CACGGACCCT 
CGATTCCAGA GATCTAGGTC GGAGCCTTCG CCAGAGGTCC CGGACAGCCC ATCCAGCTTC 
TCTCCCAATA AGAACAGGAG AGCTCCGGAG GAGTGGGCCA AGAATGAAGG ACTGATGCCT 
CGGAGTCTGT CCTTTTCCGG CCCTCGGTAC AGCCGCAGCA GGACGCCACC CTCTGCGGCC 
AGCAGCAGGT ACAGCATGAG GAACCGCATC CAAAGCAGCC CCATGACAGT CATCTCTGAG 
GGAGAGGGAG AGCCTGCCGA GCCCGCAGAC AACAAAGTAC GCAGGACCCT GGACACTACG 
AGGGGATGCT CTCTGGACAG ACTGGCGGGA CAGGAGACGG ACCGGGGCAG CCCGCTGTGT 
AGCGAGGAGC CTGCCTCTGT GGATGGGATA GGAAGGGCTA AGGACGACGG TCCCTCGGAG 
CAGTGTAGGG GTCCTGAACA GAAGAGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002728671.1
CDS	2175..6944
Translation	MVVFVNAKIKSFFKLFKKKTVSNLDEESRWTVHYTAPWHQQENVFLPSTRPPCVEDLHRQAKLNLKSVLRECDKLRRDGCRSSQYYSQGPTFAASSSPGDDYQEEDAETDRKYSLSSSEEERFIGIRRPKTPTSGDFSDLHTQTNWTKSLPLPTPEEKTRQQAQTVQADVVPINITGENFDRQASLRRSLIYTDTLVRRPKKVKRRKTISGIPDIIQKELASATGQEDDGSTHSLYSPDHYSTLGRLDSYRSTGQCLETRDSGCQTEEVKVIPPSVRRIRAHKGLGIAAQMSHLSGSSGNMSVLSDSAGIVFPSRLNNDTGFHSLPRAGARASTYSLEARMGALGSAEDTDDTFPYRGGSSQGPENFAHLGGASSTGMLSRPKSQQLRLLESPACVVSPHAAYSTSVIPNATLLSSSEVIVIHTAQSAGQLDSRVPGSSSYSKIKPRDHPTPRCSARDERQSPRHHWNEGHIIHSQALASAAPGATALLSLRDSEVSLNAPAINRENGSQAILYHCRSNLAFPAHPPDVDGKSECSSSGDRGCGSSESWEYKASSNGRASPLKPHLATPGCSTPTSNMSSCSLDQASLKEDARSLYSEDHDGYYMTAQGDSRHEAGSAYGISDGFGNPRHSTVNVFDGRAQRSQGDQATQQDRILSRNISLKKAKKPPPPPSRTDSLRRIPKKNNQTNGQVLNESLIASLQHSLQLSLPGQGSGSPSQSPCSDCEEPWLPRSRSQSIVSEGSSLTSTTTPNVYSLCGLTPSQSDTSSVKSEYADPWGYYIDYTTLQGDPGNPTGGCPASSEAATGNGPVRHIQEGSRAPVPQVPGCSVKPKITSPEKSQRVTSPSSGYSSQSNTPTALTPVPVFLKSMSPANGKGKAKPKVPERKSSLISSVSISSSSTSLSSNTSTEGSGTVKKLDSALASALAPPPPPLPALPSPSLANKSPFLPPPPPLADCSEGSPLPPSPVFPPPPPEALVPFCSPTDRCLSPSPTAFSPPLPRCSPPLPAPPPFLPPSEPPPAPPLDPKFMKENRPFIKNSSQSESSREALRRPANKEEGCRPSMPLITTEALQMVQLRPVRKNSGAETVLFSEPSAQEPRTPTAPQYHLKPSAFLKSRNSINEMESESQAASVTSSLPMPAKSQSQGDHDSAVERGGLQSCSDGAPGPGPSLRTALLPDSSPSRKPPPISKKPKLFLVVPPPQRDFTAEPTENGSEAFSGVPSPTGAEGEAGQSQEEKSSPASQAGSHATAPTSGSTVLERGTAGSLSPGVVEANAPMVQPSTSPGPAQEEAGENRVDGERNVKSCASPRGGEAGLPEPNTAGPSSDTADVSKEGGNDEVLTPTRPRTTEDLFAAIHRSKRKVLGRKDSEDDHTRNHSPSPPVTPTGAAPSLASPKQVGSIQRSLKKSSTSSDNFKALLLKKGSRSDTSARMSAAEMLKSTDPRFQRSRSEPSPEVPDSPSSFSPNKNRRAPEEWAKNEGLMPRSLSFSGPRYSRSRTPPSAASSRYSMRNRIQSSPMTVISEGEGEPAEPADNKVRRTLDTTRGCSLDRLAGQETDRGSPLCSEEPASVDGIGRAKDDGPSEQCRGPEQKS

Target ORF information:

RefSeq Version	XM_002728625.5
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus NHS-like 1 (Nhsl1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002728625.5

ORF Insert Sequence:

ATGGTGGTCT TCGTTAATGC GAAGATTAAA TCTTTCTTTA AACTTTTTAA GAAGAAAACG 
GTTTCCAACT TAGACGAGGA GAGCCGATGG ACCGTCCACT ATACGGCGCC GTGGCACCAG 
CAGGAGAATG TGTTCCTCCC CTCCACAAGA CCCCCCTGTG TTGAGGACCT GCACCGCCAA 
GCGAAGCTCA ACCTCAAATC AGTGCTGAGG GAATGTGACA AGCTGAGGCG CGATGGCTGC 
CGAAGTTCTC AGTACTATTC TCAGGGACCC ACCTTTGCAG CCAGCTCCAG CCCAGGCGAT 
GACTATCAAG AGGAAGATGC AGAAACAGAC CGAAAGTATT CTCTCTCTTC ATCTGAAGAA 
GAAAGATTTA TTGGTATCAG GAGACCTAAA ACGCCAACCT CAGGTGACTT CTCTGACCTT 
CATACTCAGA CAAACTGGAC CAAGTCCCTG CCGCTGCCAA CACCAGAAGA GAAGACACGA 
CAGCAAGCCC AGACAGTCCA GGCTGATGTG GTTCCTATTA ACATAACTGG GGAGAATTTT 
GATCGTCAGG CCAGCCTTCG GCGGTCTCTA ATTTACACTG ACACTCTGGT AAGACGACCG 
AAGAAAGTCA AAAGGAGAAA GACTATTTCA GGAATCCCTG ACATCATTCA GAAGGAGCTA 
GCGTCAGCCA CGGGCCAAGA GGATGATGGT AGCACCCATT CTCTATACTC CCCAGACCAC 
TACTCTACAT TAGGAAGGCT TGATAGCTAT CGGTCCACGG GGCAATGCTT GGAAACCAGG 
GACTCTGGCT GTCAGACAGA AGAAGTGAAA GTCATCCCAC CGTCAGTGAG AAGGATCAGG 
GCTCACAAGG GGCTGGGCAT TGCTGCCCAG ATGAGCCACC TCTCGGGTTC CTCTGGCAAC 
ATGTCTGTGC TGAGCGACTC GGCTGGTATC GTGTTCCCAT CTCGCCTCAA TAACGATACT 
GGTTTCCACA GCCTTCCACG CGCTGGCGCA AGGGCAAGCA CGTATTCCCT GGAGGCAAGG 
ATGGGTGCCC TGGGCTCTGC TGAAGATACG GATGATACTT TTCCCTACCG AGGGGGTAGC 
TCCCAGGGGC CTGAAAACTT TGCACATTTA GGGGGTGCCT CGAGTACCGG GATGCTTTCG 
AGGCCCAAAT CCCAGCAGCT GAGGCTCTTG GAGAGCCCAG CGTGTGTGGT TTCACCGCAC 
GCGGCCTACT CTACCAGCGT CATCCCAAAC GCCACACTGC TGTCCTCTTC GGAGGTTATT 
GTCATCCACA CCGCTCAGAG TGCAGGACAG CTGGACAGTA GAGTCCCCGG CTCATCCTCA 
TACTCAAAGA TAAAACCCAG AGACCATCCC ACACCCAGGT GCTCTGCGAG AGATGAGCGC 
CAGTCCCCAC GACATCACTG GAACGAGGGT CATATCATTC ACTCACAGGC TTTAGCCTCC 
GCTGCCCCCG GTGCCACCGC ACTGTTATCC CTCCGTGACT CAGAGGTCTC TCTCAATGCC 
CCAGCTATCA ATAGGGAGAA CGGGTCCCAA GCCATACTTT ATCACTGTAG AAGCAACCTG 
GCCTTTCCTG CCCACCCTCC GGATGTGGAT GGCAAGAGCG AGTGCAGTTC TTCAGGGGAC 
AGGGGATGTG GCAGTTCTGA GTCCTGGGAA TACAAAGCCT CCAGCAATGG GCGGGCTTCC 
CCACTGAAGC CGCACCTTGC GACTCCCGGT TGCTCTACCC CCACAAGTAA CATGAGCAGC 
TGCAGCTTGG ACCAAGCATC CCTCAAGGAG GATGCCAGGT CCCTGTATTC AGAGGACCAC 
GATGGTTACT ATATGACCGC CCAGGGTGAT TCTAGACACG AGGCTGGGAG CGCGTACGGT 
ATCAGTGATG GCTTTGGAAA CCCCAGGCAC AGCACGGTCA ATGTTTTTGA CGGAAGGGCT 
CAGAGAAGCC AAGGGGATCA GGCCACTCAG CAGGATAGAA TCCTCTCAAG AAACATCTCT 
CTGAAGAAAG CAAAAAAACC ACCACCGCCA CCATCCCGGA CAGATTCTCT GCGAAGGATT 
CCCAAGAAGA ACAACCAGAC AAATGGGCAG GTGCTCAACG AGAGCCTGAT CGCCTCACTC 
CAGCATTCTC TGCAGCTAAG CCTCCCTGGC CAAGGCAGCG GTTCCCCTTC CCAGAGCCCC 
TGCAGTGACT GTGAGGAACC CTGGCTCCCC AGGTCCAGAA GCCAGAGCAT TGTTAGCGAG 
GGTAGTAGCT TGACCTCCAC CACCACTCCC AACGTGTATT CTCTGTGCGG GCTCACTCCA 
TCTCAGAGCG ACACAAGCAG CGTCAAGTCG GAATACGCAG ACCCCTGGGG CTACTACATT 
GACTACACAA CTTTGCAGGG AGATCCAGGG AACCCCACAG GGGGCTGCCC AGCCAGTAGT 
GAGGCAGCCA CTGGAAATGG GCCAGTGCGC CACATCCAGG AGGGGTCGAG AGCCCCAGTG 
CCCCAAGTGC CGGGCTGCTC CGTGAAACCA AAGATCACCT CACCGGAGAA GTCACAGAGA 
GTCACCTCTC CATCCAGTGG GTATTCTAGC CAGTCGAATA CACCCACAGC ACTCACCCCC 
GTGCCTGTGT TTTTAAAATC GATGTCACCA GCAAATGGGA AGGGGAAGGC CAAGCCCAAG 
GTACCAGAAA GAAAATCGTC TCTGATCTCT TCAGTGTCCA TCTCCTCATC GTCCACTTCC 
CTCTCCTCTA ATACCTCTAC GGAAGGAAGT GGGACCGTGA AGAAACTGGA TTCAGCCCTG 
GCCTCGGCCC TTGCTCCTCC CCCTCCTCCT CTTCCCGCGC TGCCTTCTCC GAGTCTGGCA 
AACAAGTCCC CCTTCCTTCC ACCTCCTCCT CCTCTAGCAG ATTGCTCCGA GGGCTCTCCC 
TTACCGCCCT CTCCTGTGTT CCCCCCTCCA CCACCAGAAG CCCTTGTTCC CTTCTGCTCC 
CCCACTGACA GGTGCCTTTC TCCTTCCCCC ACAGCTTTTA GCCCCCCTCT TCCAAGATGC 
TCACCTCCCT TGCCAGCACC TCCACCTTTC TTGCCCCCAT CAGAACCCCC ACCTGCTCCG 
CCTCTGGACC CCAAATTCAT GAAAGAAAAC AGGCCCTTTA TCAAAAACTC TAGCCAGTCC 
GAATCCTCTA GGGAGGCCCT CAGGCGGCCT GCCAACAAGG AGGAGGGCTG CCGACCCTCG 
ATGCCCCTGA TCACCACAGA AGCTTTGCAG ATGGTGCAGT TGAGGCCAGT GAGGAAGAAC 
TCAGGTGCTG AGACGGTCCT GTTTTCTGAA CCATCAGCTC AGGAACCACG AACCCCGACT 
GCTCCACAGT ATCACTTAAA GCCATCTGCT TTCCTCAAAT CCCGAAATAG CATAAATGAA 
ATGGAGAGTG AAAGCCAGGC TGCCTCTGTG ACGAGCTCGC TTCCGATGCC TGCCAAGAGC 
CAGAGTCAGG GTGACCATGA CAGTGCAGTC GAGCGTGGCG GCCTTCAGAG CTGCAGCGAT 
GGAGCTCCAG GCCCGGGTCC CAGCCTCAGG ACCGCTCTGC TCCCAGACTC TTCACCCAGC 
AGGAAGCCAC CCCCCATCTC CAAGAAGCCC AAACTGTTCC TGGTGGTACC ACCTCCGCAG 
AGAGATTTCA CAGCGGAGCC CACAGAGAAC GGGAGCGAAG CTTTCTCAGG CGTGCCCAGC 
CCTACCGGGG CAGAGGGGGA AGCTGGGCAG AGCCAAGAGG AGAAATCCAG CCCAGCATCC 
CAAGCTGGCT CTCATGCTAC GGCCCCCACC TCCGGCAGTA CGGTTCTAGA AAGAGGAACT 
GCAGGGTCCT TGTCCCCTGG CGTTGTGGAA GCTAATGCCC CCATGGTCCA ACCTAGTACA 
TCACCGGGCC CCGCACAGGA AGAGGCAGGA GAGAACCGCG TGGATGGTGA GAGAAATGTG 
AAGAGCTGCG CGTCTCCACG GGGCGGAGAA GCTGGGTTGC CGGAACCCAA CACAGCTGGT 
CCGTCTTCAG ACACGGCAGA CGTTTCTAAA GAAGGAGGGA ACGATGAGGT GCTGACCCCT 
ACCAGACCCA GGACAACGGA AGACCTGTTT GCAGCCATTC ACAGATCCAA AAGGAAAGTC 
CTTGGTCGGA AGGACTCCGA AGATGATCAC ACTCGAAATC ATTCTCCCTC CCCGCCTGTG 
ACACCCACAG GTGCTGCCCC TAGCCTGGCC TCTCCAAAGC AAGTAGGGTC CATCCAGCGA 
AGCCTAAAAA AGAGCAGTAC CAGCAGCGAC AACTTCAAAG CTCTGCTGCT CAAGAAGGGG 
AGTCGCTCGG ACACCAGCGC TCGCATGTCC GCGGCGGAGA TGCTCAAGTC CACGGACCCT 
CGATTCCAGA GATCTAGGTC GGAGCCTTCG CCAGAGGTCC CGGACAGCCC ATCCAGCTTC 
TCTCCCAATA AGAACAGGAG AGCTCCGGAG GAGTGGGCCA AGAATGAAGG ACTGATGCCT 
CGGAGTCTGT CCTTTTCCGG CCCTCGGTAC AGCCGCAGCA GGACGCCACC CTCTGCGGCC 
AGCAGCAGGT ACAGCATGAG GAACCGCATC CAAAGCAGCC CCATGACAGT CATCTCTGAG 
GGAGAGGGAG AGCCTGCCGA GCCCGCAGAC AACAAAGTAC GCAGGACCCT GGACACTACG 
AGGGGATGCT CTCTGGACAG ACTGGCGGGA CAGGAGACGG ACCGGGGCAG CCCGCTGTGT 
AGCGAGGAGC CTGCCTCTGT GGATGGGATA GGAAGGGCTA AGGACGACGG TCCCTCGGAG 
CAGTGTAGGG GTCCTGAACA GAAGAGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nhsl1 ( XM_002725449.5 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_002725495.1 Rattus norvegicus NHS-like 1 (Nhsl1), transcript variant X5, mRNA.

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CloneID	ORa13978
Clone ID Related Accession (Same CDS sequence)	XM_002728625.5 , XM_002725449.5
Accession Version	XM_002725449.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4770bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	NHS-like protein 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (AC_000069.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_002725449.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGTCT TCGTTAATGC GAAGATTAAA TCTTTCTTTA AACTTTTTAA GAAGAAAACG 
GTTTCCAACT TAGACGAGGA GAGCCGATGG ACCGTCCACT ATACGGCGCC GTGGCACCAG 
CAGGAGAATG TGTTCCTCCC CTCCACAAGA CCCCCCTGTG TTGAGGACCT GCACCGCCAA 
GCGAAGCTCA ACCTCAAATC AGTGCTGAGG GAATGTGACA AGCTGAGGCG CGATGGCTGC 
CGAAGTTCTC AGTACTATTC TCAGGGACCC ACCTTTGCAG CCAGCTCCAG CCCAGGCGAT 
GACTATCAAG AGGAAGATGC AGAAACAGAC CGAAAGTATT CTCTCTCTTC ATCTGAAGAA 
GAAAGATTTA TTGGTATCAG GAGACCTAAA ACGCCAACCT CAGGTGACTT CTCTGACCTT 
CATACTCAGA CAAACTGGAC CAAGTCCCTG CCGCTGCCAA CACCAGAAGA GAAGACACGA 
CAGCAAGCCC AGACAGTCCA GGCTGATGTG GTTCCTATTA ACATAACTGG GGAGAATTTT 
GATCGTCAGG CCAGCCTTCG GCGGTCTCTA ATTTACACTG ACACTCTGGT AAGACGACCG 
AAGAAAGTCA AAAGGAGAAA GACTATTTCA GGAATCCCTG ACATCATTCA GAAGGAGCTA 
GCGTCAGCCA CGGGCCAAGA GGATGATGGT AGCACCCATT CTCTATACTC CCCAGACCAC 
TACTCTACAT TAGGAAGGCT TGATAGCTAT CGGTCCACGG GGCAATGCTT GGAAACCAGG 
GACTCTGGCT GTCAGACAGA AGAAGTGAAA GTCATCCCAC CGTCAGTGAG AAGGATCAGG 
GCTCACAAGG GGCTGGGCAT TGCTGCCCAG ATGAGCCACC TCTCGGGTTC CTCTGGCAAC 
ATGTCTGTGC TGAGCGACTC GGCTGGTATC GTGTTCCCAT CTCGCCTCAA TAACGATACT 
GGTTTCCACA GCCTTCCACG CGCTGGCGCA AGGGCAAGCA CGTATTCCCT GGAGGCAAGG 
ATGGGTGCCC TGGGCTCTGC TGAAGATACG GATGATACTT TTCCCTACCG AGGGGGTAGC 
TCCCAGGGGC CTGAAAACTT TGCACATTTA GGGGGTGCCT CGAGTACCGG GATGCTTTCG 
AGGCCCAAAT CCCAGCAGCT GAGGCTCTTG GAGAGCCCAG CGTGTGTGGT TTCACCGCAC 
GCGGCCTACT CTACCAGCGT CATCCCAAAC GCCACACTGC TGTCCTCTTC GGAGGTTATT 
GTCATCCACA CCGCTCAGAG TGCAGGACAG CTGGACAGTA GAGTCCCCGG CTCATCCTCA 
TACTCAAAGA TAAAACCCAG AGACCATCCC ACACCCAGGT GCTCTGCGAG AGATGAGCGC 
CAGTCCCCAC GACATCACTG GAACGAGGGT CATATCATTC ACTCACAGGC TTTAGCCTCC 
GCTGCCCCCG GTGCCACCGC ACTGTTATCC CTCCGTGACT CAGAGGTCTC TCTCAATGCC 
CCAGCTATCA ATAGGGAGAA CGGGTCCCAA GCCATACTTT ATCACTGTAG AAGCAACCTG 
GCCTTTCCTG CCCACCCTCC GGATGTGGAT GGCAAGAGCG AGTGCAGTTC TTCAGGGGAC 
AGGGGATGTG GCAGTTCTGA GTCCTGGGAA TACAAAGCCT CCAGCAATGG GCGGGCTTCC 
CCACTGAAGC CGCACCTTGC GACTCCCGGT TGCTCTACCC CCACAAGTAA CATGAGCAGC 
TGCAGCTTGG ACCAAGCATC CCTCAAGGAG GATGCCAGGT CCCTGTATTC AGAGGACCAC 
GATGGTTACT ATATGACCGC CCAGGGTGAT TCTAGACACG AGGCTGGGAG CGCGTACGGT 
ATCAGTGATG GCTTTGGAAA CCCCAGGCAC AGCACGGTCA ATGTTTTTGA CGGAAGGGCT 
CAGAGAAGCC AAGGGGATCA GGCCACTCAG CAGGATAGAA TCCTCTCAAG AAACATCTCT 
CTGAAGAAAG CAAAAAAACC ACCACCGCCA CCATCCCGGA CAGATTCTCT GCGAAGGATT 
CCCAAGAAGA ACAACCAGAC AAATGGGCAG GTGCTCAACG AGAGCCTGAT CGCCTCACTC 
CAGCATTCTC TGCAGCTAAG CCTCCCTGGC CAAGGCAGCG GTTCCCCTTC CCAGAGCCCC 
TGCAGTGACT GTGAGGAACC CTGGCTCCCC AGGTCCAGAA GCCAGAGCAT TGTTAGCGAG 
GGTAGTAGCT TGACCTCCAC CACCACTCCC AACGTGTATT CTCTGTGCGG GCTCACTCCA 
TCTCAGAGCG ACACAAGCAG CGTCAAGTCG GAATACGCAG ACCCCTGGGG CTACTACATT 
GACTACACAA CTTTGCAGGG AGATCCAGGG AACCCCACAG GGGGCTGCCC AGCCAGTAGT 
GAGGCAGCCA CTGGAAATGG GCCAGTGCGC CACATCCAGG AGGGGTCGAG AGCCCCAGTG 
CCCCAAGTGC CGGGCTGCTC CGTGAAACCA AAGATCACCT CACCGGAGAA GTCACAGAGA 
GTCACCTCTC CATCCAGTGG GTATTCTAGC CAGTCGAATA CACCCACAGC ACTCACCCCC 
GTGCCTGTGT TTTTAAAATC GATGTCACCA GCAAATGGGA AGGGGAAGGC CAAGCCCAAG 
GTACCAGAAA GAAAATCGTC TCTGATCTCT TCAGTGTCCA TCTCCTCATC GTCCACTTCC 
CTCTCCTCTA ATACCTCTAC GGAAGGAAGT GGGACCGTGA AGAAACTGGA TTCAGCCCTG 
GCCTCGGCCC TTGCTCCTCC CCCTCCTCCT CTTCCCGCGC TGCCTTCTCC GAGTCTGGCA 
AACAAGTCCC CCTTCCTTCC ACCTCCTCCT CCTCTAGCAG ATTGCTCCGA GGGCTCTCCC 
TTACCGCCCT CTCCTGTGTT CCCCCCTCCA CCACCAGAAG CCCTTGTTCC CTTCTGCTCC 
CCCACTGACA GGTGCCTTTC TCCTTCCCCC ACAGCTTTTA GCCCCCCTCT TCCAAGATGC 
TCACCTCCCT TGCCAGCACC TCCACCTTTC TTGCCCCCAT CAGAACCCCC ACCTGCTCCG 
CCTCTGGACC CCAAATTCAT GAAAGAAAAC AGGCCCTTTA TCAAAAACTC TAGCCAGTCC 
GAATCCTCTA GGGAGGCCCT CAGGCGGCCT GCCAACAAGG AGGAGGGCTG CCGACCCTCG 
ATGCCCCTGA TCACCACAGA AGCTTTGCAG ATGGTGCAGT TGAGGCCAGT GAGGAAGAAC 
TCAGGTGCTG AGACGGTCCT GTTTTCTGAA CCATCAGCTC AGGAACCACG AACCCCGACT 
GCTCCACAGT ATCACTTAAA GCCATCTGCT TTCCTCAAAT CCCGAAATAG CATAAATGAA 
ATGGAGAGTG AAAGCCAGGC TGCCTCTGTG ACGAGCTCGC TTCCGATGCC TGCCAAGAGC 
CAGAGTCAGG GTGACCATGA CAGTGCAGTC GAGCGTGGCG GCCTTCAGAG CTGCAGCGAT 
GGAGCTCCAG GCCCGGGTCC CAGCCTCAGG ACCGCTCTGC TCCCAGACTC TTCACCCAGC 
AGGAAGCCAC CCCCCATCTC CAAGAAGCCC AAACTGTTCC TGGTGGTACC ACCTCCGCAG 
AGAGATTTCA CAGCGGAGCC CACAGAGAAC GGGAGCGAAG CTTTCTCAGG CGTGCCCAGC 
CCTACCGGGG CAGAGGGGGA AGCTGGGCAG AGCCAAGAGG AGAAATCCAG CCCAGCATCC 
CAAGCTGGCT CTCATGCTAC GGCCCCCACC TCCGGCAGTA CGGTTCTAGA AAGAGGAACT 
GCAGGGTCCT TGTCCCCTGG CGTTGTGGAA GCTAATGCCC CCATGGTCCA ACCTAGTACA 
TCACCGGGCC CCGCACAGGA AGAGGCAGGA GAGAACCGCG TGGATGGTGA GAGAAATGTG 
AAGAGCTGCG CGTCTCCACG GGGCGGAGAA GCTGGGTTGC CGGAACCCAA CACAGCTGGT 
CCGTCTTCAG ACACGGCAGA CGTTTCTAAA GAAGGAGGGA ACGATGAGGT GCTGACCCCT 
ACCAGACCCA GGACAACGGA AGACCTGTTT GCAGCCATTC ACAGATCCAA AAGGAAAGTC 
CTTGGTCGGA AGGACTCCGA AGATGATCAC ACTCGAAATC ATTCTCCCTC CCCGCCTGTG 
ACACCCACAG GTGCTGCCCC TAGCCTGGCC TCTCCAAAGC AAGTAGGGTC CATCCAGCGA 
AGCCTAAAAA AGAGCAGTAC CAGCAGCGAC AACTTCAAAG CTCTGCTGCT CAAGAAGGGG 
AGTCGCTCGG ACACCAGCGC TCGCATGTCC GCGGCGGAGA TGCTCAAGTC CACGGACCCT 
CGATTCCAGA GATCTAGGTC GGAGCCTTCG CCAGAGGTCC CGGACAGCCC ATCCAGCTTC 
TCTCCCAATA AGAACAGGAG AGCTCCGGAG GAGTGGGCCA AGAATGAAGG ACTGATGCCT 
CGGAGTCTGT CCTTTTCCGG CCCTCGGTAC AGCCGCAGCA GGACGCCACC CTCTGCGGCC 
AGCAGCAGGT ACAGCATGAG GAACCGCATC CAAAGCAGCC CCATGACAGT CATCTCTGAG 
GGAGAGGGAG AGCCTGCCGA GCCCGCAGAC AACAAAGTAC GCAGGACCCT GGACACTACG 
AGGGGATGCT CTCTGGACAG ACTGGCGGGA CAGGAGACGG ACCGGGGCAG CCCGCTGTGT 
AGCGAGGAGC CTGCCTCTGT GGATGGGATA GGAAGGGCTA AGGACGACGG TCCCTCGGAG 
CAGTGTAGGG GTCCTGAACA GAAGAGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002725495.1
CDS	2175..6944
Translation	MVVFVNAKIKSFFKLFKKKTVSNLDEESRWTVHYTAPWHQQENVFLPSTRPPCVEDLHRQAKLNLKSVLRECDKLRRDGCRSSQYYSQGPTFAASSSPGDDYQEEDAETDRKYSLSSSEEERFIGIRRPKTPTSGDFSDLHTQTNWTKSLPLPTPEEKTRQQAQTVQADVVPINITGENFDRQASLRRSLIYTDTLVRRPKKVKRRKTISGIPDIIQKELASATGQEDDGSTHSLYSPDHYSTLGRLDSYRSTGQCLETRDSGCQTEEVKVIPPSVRRIRAHKGLGIAAQMSHLSGSSGNMSVLSDSAGIVFPSRLNNDTGFHSLPRAGARASTYSLEARMGALGSAEDTDDTFPYRGGSSQGPENFAHLGGASSTGMLSRPKSQQLRLLESPACVVSPHAAYSTSVIPNATLLSSSEVIVIHTAQSAGQLDSRVPGSSSYSKIKPRDHPTPRCSARDERQSPRHHWNEGHIIHSQALASAAPGATALLSLRDSEVSLNAPAINRENGSQAILYHCRSNLAFPAHPPDVDGKSECSSSGDRGCGSSESWEYKASSNGRASPLKPHLATPGCSTPTSNMSSCSLDQASLKEDARSLYSEDHDGYYMTAQGDSRHEAGSAYGISDGFGNPRHSTVNVFDGRAQRSQGDQATQQDRILSRNISLKKAKKPPPPPSRTDSLRRIPKKNNQTNGQVLNESLIASLQHSLQLSLPGQGSGSPSQSPCSDCEEPWLPRSRSQSIVSEGSSLTSTTTPNVYSLCGLTPSQSDTSSVKSEYADPWGYYIDYTTLQGDPGNPTGGCPASSEAATGNGPVRHIQEGSRAPVPQVPGCSVKPKITSPEKSQRVTSPSSGYSSQSNTPTALTPVPVFLKSMSPANGKGKAKPKVPERKSSLISSVSISSSSTSLSSNTSTEGSGTVKKLDSALASALAPPPPPLPALPSPSLANKSPFLPPPPPLADCSEGSPLPPSPVFPPPPPEALVPFCSPTDRCLSPSPTAFSPPLPRCSPPLPAPPPFLPPSEPPPAPPLDPKFMKENRPFIKNSSQSESSREALRRPANKEEGCRPSMPLITTEALQMVQLRPVRKNSGAETVLFSEPSAQEPRTPTAPQYHLKPSAFLKSRNSINEMESESQAASVTSSLPMPAKSQSQGDHDSAVERGGLQSCSDGAPGPGPSLRTALLPDSSPSRKPPPISKKPKLFLVVPPPQRDFTAEPTENGSEAFSGVPSPTGAEGEAGQSQEEKSSPASQAGSHATAPTSGSTVLERGTAGSLSPGVVEANAPMVQPSTSPGPAQEEAGENRVDGERNVKSCASPRGGEAGLPEPNTAGPSSDTADVSKEGGNDEVLTPTRPRTTEDLFAAIHRSKRKVLGRKDSEDDHTRNHSPSPPVTPTGAAPSLASPKQVGSIQRSLKKSSTSSDNFKALLLKKGSRSDTSARMSAAEMLKSTDPRFQRSRSEPSPEVPDSPSSFSPNKNRRAPEEWAKNEGLMPRSLSFSGPRYSRSRTPPSAASSRYSMRNRIQSSPMTVISEGEGEPAEPADNKVRRTLDTTRGCSLDRLAGQETDRGSPLCSEEPASVDGIGRAKDDGPSEQCRGPEQKS

Target ORF information:

RefSeq Version	XM_002725449.5
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus NHS-like 1 (Nhsl1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002725449.5

ORF Insert Sequence:

ATGGTGGTCT TCGTTAATGC GAAGATTAAA TCTTTCTTTA AACTTTTTAA GAAGAAAACG 
GTTTCCAACT TAGACGAGGA GAGCCGATGG ACCGTCCACT ATACGGCGCC GTGGCACCAG 
CAGGAGAATG TGTTCCTCCC CTCCACAAGA CCCCCCTGTG TTGAGGACCT GCACCGCCAA 
GCGAAGCTCA ACCTCAAATC AGTGCTGAGG GAATGTGACA AGCTGAGGCG CGATGGCTGC 
CGAAGTTCTC AGTACTATTC TCAGGGACCC ACCTTTGCAG CCAGCTCCAG CCCAGGCGAT 
GACTATCAAG AGGAAGATGC AGAAACAGAC CGAAAGTATT CTCTCTCTTC ATCTGAAGAA 
GAAAGATTTA TTGGTATCAG GAGACCTAAA ACGCCAACCT CAGGTGACTT CTCTGACCTT 
CATACTCAGA CAAACTGGAC CAAGTCCCTG CCGCTGCCAA CACCAGAAGA GAAGACACGA 
CAGCAAGCCC AGACAGTCCA GGCTGATGTG GTTCCTATTA ACATAACTGG GGAGAATTTT 
GATCGTCAGG CCAGCCTTCG GCGGTCTCTA ATTTACACTG ACACTCTGGT AAGACGACCG 
AAGAAAGTCA AAAGGAGAAA GACTATTTCA GGAATCCCTG ACATCATTCA GAAGGAGCTA 
GCGTCAGCCA CGGGCCAAGA GGATGATGGT AGCACCCATT CTCTATACTC CCCAGACCAC 
TACTCTACAT TAGGAAGGCT TGATAGCTAT CGGTCCACGG GGCAATGCTT GGAAACCAGG 
GACTCTGGCT GTCAGACAGA AGAAGTGAAA GTCATCCCAC CGTCAGTGAG AAGGATCAGG 
GCTCACAAGG GGCTGGGCAT TGCTGCCCAG ATGAGCCACC TCTCGGGTTC CTCTGGCAAC 
ATGTCTGTGC TGAGCGACTC GGCTGGTATC GTGTTCCCAT CTCGCCTCAA TAACGATACT 
GGTTTCCACA GCCTTCCACG CGCTGGCGCA AGGGCAAGCA CGTATTCCCT GGAGGCAAGG 
ATGGGTGCCC TGGGCTCTGC TGAAGATACG GATGATACTT TTCCCTACCG AGGGGGTAGC 
TCCCAGGGGC CTGAAAACTT TGCACATTTA GGGGGTGCCT CGAGTACCGG GATGCTTTCG 
AGGCCCAAAT CCCAGCAGCT GAGGCTCTTG GAGAGCCCAG CGTGTGTGGT TTCACCGCAC 
GCGGCCTACT CTACCAGCGT CATCCCAAAC GCCACACTGC TGTCCTCTTC GGAGGTTATT 
GTCATCCACA CCGCTCAGAG TGCAGGACAG CTGGACAGTA GAGTCCCCGG CTCATCCTCA 
TACTCAAAGA TAAAACCCAG AGACCATCCC ACACCCAGGT GCTCTGCGAG AGATGAGCGC 
CAGTCCCCAC GACATCACTG GAACGAGGGT CATATCATTC ACTCACAGGC TTTAGCCTCC 
GCTGCCCCCG GTGCCACCGC ACTGTTATCC CTCCGTGACT CAGAGGTCTC TCTCAATGCC 
CCAGCTATCA ATAGGGAGAA CGGGTCCCAA GCCATACTTT ATCACTGTAG AAGCAACCTG 
GCCTTTCCTG CCCACCCTCC GGATGTGGAT GGCAAGAGCG AGTGCAGTTC TTCAGGGGAC 
AGGGGATGTG GCAGTTCTGA GTCCTGGGAA TACAAAGCCT CCAGCAATGG GCGGGCTTCC 
CCACTGAAGC CGCACCTTGC GACTCCCGGT TGCTCTACCC CCACAAGTAA CATGAGCAGC 
TGCAGCTTGG ACCAAGCATC CCTCAAGGAG GATGCCAGGT CCCTGTATTC AGAGGACCAC 
GATGGTTACT ATATGACCGC CCAGGGTGAT TCTAGACACG AGGCTGGGAG CGCGTACGGT 
ATCAGTGATG GCTTTGGAAA CCCCAGGCAC AGCACGGTCA ATGTTTTTGA CGGAAGGGCT 
CAGAGAAGCC AAGGGGATCA GGCCACTCAG CAGGATAGAA TCCTCTCAAG AAACATCTCT 
CTGAAGAAAG CAAAAAAACC ACCACCGCCA CCATCCCGGA CAGATTCTCT GCGAAGGATT 
CCCAAGAAGA ACAACCAGAC AAATGGGCAG GTGCTCAACG AGAGCCTGAT CGCCTCACTC 
CAGCATTCTC TGCAGCTAAG CCTCCCTGGC CAAGGCAGCG GTTCCCCTTC CCAGAGCCCC 
TGCAGTGACT GTGAGGAACC CTGGCTCCCC AGGTCCAGAA GCCAGAGCAT TGTTAGCGAG 
GGTAGTAGCT TGACCTCCAC CACCACTCCC AACGTGTATT CTCTGTGCGG GCTCACTCCA 
TCTCAGAGCG ACACAAGCAG CGTCAAGTCG GAATACGCAG ACCCCTGGGG CTACTACATT 
GACTACACAA CTTTGCAGGG AGATCCAGGG AACCCCACAG GGGGCTGCCC AGCCAGTAGT 
GAGGCAGCCA CTGGAAATGG GCCAGTGCGC CACATCCAGG AGGGGTCGAG AGCCCCAGTG 
CCCCAAGTGC CGGGCTGCTC CGTGAAACCA AAGATCACCT CACCGGAGAA GTCACAGAGA 
GTCACCTCTC CATCCAGTGG GTATTCTAGC CAGTCGAATA CACCCACAGC ACTCACCCCC 
GTGCCTGTGT TTTTAAAATC GATGTCACCA GCAAATGGGA AGGGGAAGGC CAAGCCCAAG 
GTACCAGAAA GAAAATCGTC TCTGATCTCT TCAGTGTCCA TCTCCTCATC GTCCACTTCC 
CTCTCCTCTA ATACCTCTAC GGAAGGAAGT GGGACCGTGA AGAAACTGGA TTCAGCCCTG 
GCCTCGGCCC TTGCTCCTCC CCCTCCTCCT CTTCCCGCGC TGCCTTCTCC GAGTCTGGCA 
AACAAGTCCC CCTTCCTTCC ACCTCCTCCT CCTCTAGCAG ATTGCTCCGA GGGCTCTCCC 
TTACCGCCCT CTCCTGTGTT CCCCCCTCCA CCACCAGAAG CCCTTGTTCC CTTCTGCTCC 
CCCACTGACA GGTGCCTTTC TCCTTCCCCC ACAGCTTTTA GCCCCCCTCT TCCAAGATGC 
TCACCTCCCT TGCCAGCACC TCCACCTTTC TTGCCCCCAT CAGAACCCCC ACCTGCTCCG 
CCTCTGGACC CCAAATTCAT GAAAGAAAAC AGGCCCTTTA TCAAAAACTC TAGCCAGTCC 
GAATCCTCTA GGGAGGCCCT CAGGCGGCCT GCCAACAAGG AGGAGGGCTG CCGACCCTCG 
ATGCCCCTGA TCACCACAGA AGCTTTGCAG ATGGTGCAGT TGAGGCCAGT GAGGAAGAAC 
TCAGGTGCTG AGACGGTCCT GTTTTCTGAA CCATCAGCTC AGGAACCACG AACCCCGACT 
GCTCCACAGT ATCACTTAAA GCCATCTGCT TTCCTCAAAT CCCGAAATAG CATAAATGAA 
ATGGAGAGTG AAAGCCAGGC TGCCTCTGTG ACGAGCTCGC TTCCGATGCC TGCCAAGAGC 
CAGAGTCAGG GTGACCATGA CAGTGCAGTC GAGCGTGGCG GCCTTCAGAG CTGCAGCGAT 
GGAGCTCCAG GCCCGGGTCC CAGCCTCAGG ACCGCTCTGC TCCCAGACTC TTCACCCAGC 
AGGAAGCCAC CCCCCATCTC CAAGAAGCCC AAACTGTTCC TGGTGGTACC ACCTCCGCAG 
AGAGATTTCA CAGCGGAGCC CACAGAGAAC GGGAGCGAAG CTTTCTCAGG CGTGCCCAGC 
CCTACCGGGG CAGAGGGGGA AGCTGGGCAG AGCCAAGAGG AGAAATCCAG CCCAGCATCC 
CAAGCTGGCT CTCATGCTAC GGCCCCCACC TCCGGCAGTA CGGTTCTAGA AAGAGGAACT 
GCAGGGTCCT TGTCCCCTGG CGTTGTGGAA GCTAATGCCC CCATGGTCCA ACCTAGTACA 
TCACCGGGCC CCGCACAGGA AGAGGCAGGA GAGAACCGCG TGGATGGTGA GAGAAATGTG 
AAGAGCTGCG CGTCTCCACG GGGCGGAGAA GCTGGGTTGC CGGAACCCAA CACAGCTGGT 
CCGTCTTCAG ACACGGCAGA CGTTTCTAAA GAAGGAGGGA ACGATGAGGT GCTGACCCCT 
ACCAGACCCA GGACAACGGA AGACCTGTTT GCAGCCATTC ACAGATCCAA AAGGAAAGTC 
CTTGGTCGGA AGGACTCCGA AGATGATCAC ACTCGAAATC ATTCTCCCTC CCCGCCTGTG 
ACACCCACAG GTGCTGCCCC TAGCCTGGCC TCTCCAAAGC AAGTAGGGTC CATCCAGCGA 
AGCCTAAAAA AGAGCAGTAC CAGCAGCGAC AACTTCAAAG CTCTGCTGCT CAAGAAGGGG 
AGTCGCTCGG ACACCAGCGC TCGCATGTCC GCGGCGGAGA TGCTCAAGTC CACGGACCCT 
CGATTCCAGA GATCTAGGTC GGAGCCTTCG CCAGAGGTCC CGGACAGCCC ATCCAGCTTC 
TCTCCCAATA AGAACAGGAG AGCTCCGGAG GAGTGGGCCA AGAATGAAGG ACTGATGCCT 
CGGAGTCTGT CCTTTTCCGG CCCTCGGTAC AGCCGCAGCA GGACGCCACC CTCTGCGGCC 
AGCAGCAGGT ACAGCATGAG GAACCGCATC CAAAGCAGCC CCATGACAGT CATCTCTGAG 
GGAGAGGGAG AGCCTGCCGA GCCCGCAGAC AACAAAGTAC GCAGGACCCT GGACACTACG 
AGGGGATGCT CTCTGGACAG ACTGGCGGGA CAGGAGACGG ACCGGGGCAG CCCGCTGTGT 
AGCGAGGAGC CTGCCTCTGT GGATGGGATA GGAAGGGCTA AGGACGACGG TCCCTCGGAG 
CAGTGTAGGG GTCCTGAACA GAAGAGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nhsl1 ( XM_006222833.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006222895.1 Rattus norvegicus NHS-like 1 (Nhsl1), transcript variant X8, mRNA.

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CloneID	ORa13979
Clone ID Related Accession (Same CDS sequence)	XM_006222833.3 , XM_006227670.3
Accession Version	XM_006222833.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4728bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	NHS-like protein 1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (AC_000069.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006222833.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCTGCC TGAAAGCGGT TTCCAACTTA GACGAGGAGA GCCGATGGAC CGTCCACTAT 
ACGGCGCCGT GGCACCAGCA GGAGAATGTG TTCCTCCCCT CCACAAGACC CCCCTGTGTT 
GAGGACCTGC ACCGCCAAGC GAAGCTCAAC CTCAAATCAG TGCTGAGGGA ATGTGACAAG 
CTGAGGCGCG ATGGCTGCCG AAGTTCTCAG TACTATTCTC AGGGACCCAC CTTTGCAGCC 
AGCTCCAGCC CAGGCGATGA CTATCAAGAG GAAGATGCAG AAACAGACCG AAAGTATTCT 
CTCTCTTCAT CTGAAGAAGA AAGATTTATT GGTATCAGGA GACCTAAAAC GCCAACCTCA 
GGTGACTTCT CTGACCTTCA TACTCAGACA AACTGGACCA AGTCCCTGCC GCTGCCAACA 
CCAGAAGAGA AGACACGACA GCAAGCCCAG ACAGTCCAGG CTGATGTGGT TCCTATTAAC 
ATAACTGGGG AGAATTTTGA TCGTCAGGCC AGCCTTCGGC GGTCTCTAAT TTACACTGAC 
ACTCTGGTAA GACGACCGAA GAAAGTCAAA AGGAGAAAGA CTATTTCAGG AATCCCTGAC 
ATCATTCAGA AGGAGCTAGC GTCAGCCACG GGCCAAGAGG ATGATGGTAG CACCCATTCT 
CTATACTCCC CAGACCACTA CTCTACATTA GGAAGGCTTG ATAGCTATCG GTCCACGGGG 
CAATGCTTGG AAACCAGGGA CTCTGGCTGT CAGACAGAAG AAGTGAAAGT CATCCCACCG 
TCAGTGAGAA GGATCAGGGC TCACAAGGGG CTGGGCATTG CTGCCCAGAT GAGCCACCTC 
TCGGGTTCCT CTGGCAACAT GTCTGTGCTG AGCGACTCGG CTGGTATCGT GTTCCCATCT 
CGCCTCAATA ACGATACTGG TTTCCACAGC CTTCCACGCG CTGGCGCAAG GGCAAGCACG 
TATTCCCTGG AGGCAAGGAT GGGTGCCCTG GGCTCTGCTG AAGATACGGA TGATACTTTT 
CCCTACCGAG GGGGTAGCTC CCAGGGGCCT GAAAACTTTG CACATTTAGG GGGTGCCTCG 
AGTACCGGGA TGCTTTCGAG GCCCAAATCC CAGCAGCTGA GGCTCTTGGA GAGCCCAGCG 
TGTGTGGTTT CACCGCACGC GGCCTACTCT ACCAGCGTCA TCCCAAACGC CACACTGCTG 
TCCTCTTCGG AGGTTATTGT CATCCACACC GCTCAGAGTG CAGGACAGCT GGACAGTAGA 
GTCCCCGGCT CATCCTCATA CTCAAAGATA AAACCCAGAG ACCATCCCAC ACCCAGGTGC 
TCTGCGAGAG ATGAGCGCCA GTCCCCACGA CATCACTGGA ACGAGGGTCA TATCATTCAC 
TCACAGGCTT TAGCCTCCGC TGCCCCCGGT GCCACCGCAC TGTTATCCCT CCGTGACTCA 
GAGGTCTCTC TCAATGCCCC AGCTATCAAT AGGGAGAACG GGTCCCAAGC CATACTTTAT 
CACTGTAGAA GCAACCTGGC CTTTCCTGCC CACCCTCCGG ATGTGGATGG CAAGAGCGAG 
TGCAGTTCTT CAGGGGACAG GGGATGTGGC AGTTCTGAGT CCTGGGAATA CAAAGCCTCC 
AGCAATGGGC GGGCTTCCCC ACTGAAGCCG CACCTTGCGA CTCCCGGTTG CTCTACCCCC 
ACAAGTAACA TGAGCAGCTG CAGCTTGGAC CAAGCATCCC TCAAGGAGGA TGCCAGGTCC 
CTGTATTCAG AGGACCACGA TGGTTACTAT ATGACCGCCC AGGGTGATTC TAGACACGAG 
GCTGGGAGCG CGTACGGTAT CAGTGATGGC TTTGGAAACC CCAGGCACAG CACGGTCAAT 
GTTTTTGACG GAAGGGCTCA GAGAAGCCAA GGGGATCAGG CCACTCAGCA GGATAGAATC 
CTCTCAAGAA ACATCTCTCT GAAGAAAGCA AAAAAACCAC CACCGCCACC ATCCCGGACA 
GATTCTCTGC GAAGGATTCC CAAGAAGAAC AACCAGACAA ATGGGCAGGT GCTCAACGAG 
AGCCTGATCG CCTCACTCCA GCATTCTCTG CAGCTAAGCC TCCCTGGCCA AGGCAGCGGT 
TCCCCTTCCC AGAGCCCCTG CAGTGACTGT GAGGAACCCT GGCTCCCCAG GTCCAGAAGC 
CAGAGCATTG TTAGCGAGGG TAGTAGCTTG ACCTCCACCA CCACTCCCAA CGTGTATTCT 
CTGTGCGGGC TCACTCCATC TCAGAGCGAC ACAAGCAGCG TCAAGTCGGA ATACGCAGAC 
CCCTGGGGCT ACTACATTGA CTACACAACT TTGCAGGGAG ATCCAGGGAA CCCCACAGGG 
GGCTGCCCAG CCAGTAGTGA GGCAGCCACT GGAAATGGGC CAGTGCGCCA CATCCAGGAG 
GGGTCGAGAG CCCCAGTGCC CCAAGTGCCG GGCTGCTCCG TGAAACCAAA GATCACCTCA 
CCGGAGAAGT CACAGAGAGT CACCTCTCCA TCCAGTGGGT ATTCTAGCCA GTCGAATACA 
CCCACAGCAC TCACCCCCGT GCCTGTGTTT TTAAAATCGA TGTCACCAGC AAATGGGAAG 
GGGAAGGCCA AGCCCAAGGT ACCAGAAAGA AAATCGTCTC TGATCTCTTC AGTGTCCATC 
TCCTCATCGT CCACTTCCCT CTCCTCTAAT ACCTCTACGG AAGGAAGTGG GACCGTGAAG 
AAACTGGATT CAGCCCTGGC CTCGGCCCTT GCTCCTCCCC CTCCTCCTCT TCCCGCGCTG 
CCTTCTCCGA GTCTGGCAAA CAAGTCCCCC TTCCTTCCAC CTCCTCCTCC TCTAGCAGAT 
TGCTCCGAGG GCTCTCCCTT ACCGCCCTCT CCTGTGTTCC CCCCTCCACC ACCAGAAGCC 
CTTGTTCCCT TCTGCTCCCC CACTGACAGG TGCCTTTCTC CTTCCCCCAC AGCTTTTAGC 
CCCCCTCTTC CAAGATGCTC ACCTCCCTTG CCAGCACCTC CACCTTTCTT GCCCCCATCA 
GAACCCCCAC CTGCTCCGCC TCTGGACCCC AAATTCATGA AAGAAAACAG GCCCTTTATC 
AAAAACTCTA GCCAGTCCGA ATCCTCTAGG GAGGCCCTCA GGCGGCCTGC CAACAAGGAG 
GAGGGCTGCC GACCCTCGAT GCCCCTGATC ACCACAGAAG CTTTGCAGAT GGTGCAGTTG 
AGGCCAGTGA GGAAGAACTC AGGTGCTGAG ACGGTCCTGT TTTCTGAACC ATCAGCTCAG 
GAACCACGAA CCCCGACTGC TCCACAGTAT CACTTAAAGC CATCTGCTTT CCTCAAATCC 
CGAAATAGCA TAAATGAAAT GGAGAGTGAA AGCCAGGCTG CCTCTGTGAC GAGCTCGCTT 
CCGATGCCTG CCAAGAGCCA GAGTCAGGGT GACCATGACA GTGCAGTCGA GCGTGGCGGC 
CTTCAGAGCT GCAGCGATGG AGCTCCAGGC CCGGGTCCCA GCCTCAGGAC CGCTCTGCTC 
CCAGACTCTT CACCCAGCAG GAAGCCACCC CCCATCTCCA AGAAGCCCAA ACTGTTCCTG 
GTGGTACCAC CTCCGCAGAG AGATTTCACA GCGGAGCCCA CAGAGAACGG GAGCGAAGCT 
TTCTCAGGCG TGCCCAGCCC TACCGGGGCA GAGGGGGAAG CTGGGCAGAG CCAAGAGGAG 
AAATCCAGCC CAGCATCCCA AGCTGGCTCT CATGCTACGG CCCCCACCTC CGGCAGTACG 
GTTCTAGAAA GAGGAACTGC AGGGTCCTTG TCCCCTGGCG TTGTGGAAGC TAATGCCCCC 
ATGGTCCAAC CTAGTACATC ACCGGGCCCC GCACAGGAAG AGGCAGGAGA GAACCGCGTG 
GATGGTGAGA GAAATGTGAA GAGCTGCGCG TCTCCACGGG GCGGAGAAGC TGGGTTGCCG 
GAACCCAACA CAGCTGGTCC GTCTTCAGAC ACGGCAGACG TTTCTAAAGA AGGAGGGAAC 
GATGAGGTGC TGACCCCTAC CAGACCCAGG ACAACGGAAG ACCTGTTTGC AGCCATTCAC 
AGATCCAAAA GGAAAGTCCT TGGTCGGAAG GACTCCGAAG ATGATCACAC TCGAAATCAT 
TCTCCCTCCC CGCCTGTGAC ACCCACAGGT GCTGCCCCTA GCCTGGCCTC TCCAAAGCAA 
GTAGGGTCCA TCCAGCGAAG CCTAAAAAAG AGCAGTACCA GCAGCGACAA CTTCAAAGCT 
CTGCTGCTCA AGAAGGGGAG TCGCTCGGAC ACCAGCGCTC GCATGTCCGC GGCGGAGATG 
CTCAAGTCCA CGGACCCTCG ATTCCAGAGA TCTAGGTCGG AGCCTTCGCC AGAGGTCCCG 
GACAGCCCAT CCAGCTTCTC TCCCAATAAG AACAGGAGAG CTCCGGAGGA GTGGGCCAAG 
AATGAAGGAC TGATGCCTCG GAGTCTGTCC TTTTCCGGCC CTCGGTACAG CCGCAGCAGG 
ACGCCACCCT CTGCGGCCAG CAGCAGGTAC AGCATGAGGA ACCGCATCCA AAGCAGCCCC 
ATGACAGTCA TCTCTGAGGG AGAGGGAGAG CCTGCCGAGC CCGCAGACAA CAAAGTACGC 
AGGACCCTGG ACACTACGAG GGGATGCTCT CTGGACAGAC TGGCGGGACA GGAGACGGAC 
CGGGGCAGCC CGCTGTGTAG CGAGGAGCCT GCCTCTGTGG ATGGGATAGG AAGGGCTAAG 
GACGACGGTC CCTCGGAGCA GTGTAGGGGT CCTGAACAGA AGAGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006222895.1
CDS	2730..7457
Translation	MFCLKAVSNLDEESRWTVHYTAPWHQQENVFLPSTRPPCVEDLHRQAKLNLKSVLRECDKLRRDGCRSSQYYSQGPTFAASSSPGDDYQEEDAETDRKYSLSSSEEERFIGIRRPKTPTSGDFSDLHTQTNWTKSLPLPTPEEKTRQQAQTVQADVVPINITGENFDRQASLRRSLIYTDTLVRRPKKVKRRKTISGIPDIIQKELASATGQEDDGSTHSLYSPDHYSTLGRLDSYRSTGQCLETRDSGCQTEEVKVIPPSVRRIRAHKGLGIAAQMSHLSGSSGNMSVLSDSAGIVFPSRLNNDTGFHSLPRAGARASTYSLEARMGALGSAEDTDDTFPYRGGSSQGPENFAHLGGASSTGMLSRPKSQQLRLLESPACVVSPHAAYSTSVIPNATLLSSSEVIVIHTAQSAGQLDSRVPGSSSYSKIKPRDHPTPRCSARDERQSPRHHWNEGHIIHSQALASAAPGATALLSLRDSEVSLNAPAINRENGSQAILYHCRSNLAFPAHPPDVDGKSECSSSGDRGCGSSESWEYKASSNGRASPLKPHLATPGCSTPTSNMSSCSLDQASLKEDARSLYSEDHDGYYMTAQGDSRHEAGSAYGISDGFGNPRHSTVNVFDGRAQRSQGDQATQQDRILSRNISLKKAKKPPPPPSRTDSLRRIPKKNNQTNGQVLNESLIASLQHSLQLSLPGQGSGSPSQSPCSDCEEPWLPRSRSQSIVSEGSSLTSTTTPNVYSLCGLTPSQSDTSSVKSEYADPWGYYIDYTTLQGDPGNPTGGCPASSEAATGNGPVRHIQEGSRAPVPQVPGCSVKPKITSPEKSQRVTSPSSGYSSQSNTPTALTPVPVFLKSMSPANGKGKAKPKVPERKSSLISSVSISSSSTSLSSNTSTEGSGTVKKLDSALASALAPPPPPLPALPSPSLANKSPFLPPPPPLADCSEGSPLPPSPVFPPPPPEALVPFCSPTDRCLSPSPTAFSPPLPRCSPPLPAPPPFLPPSEPPPAPPLDPKFMKENRPFIKNSSQSESSREALRRPANKEEGCRPSMPLITTEALQMVQLRPVRKNSGAETVLFSEPSAQEPRTPTAPQYHLKPSAFLKSRNSINEMESESQAASVTSSLPMPAKSQSQGDHDSAVERGGLQSCSDGAPGPGPSLRTALLPDSSPSRKPPPISKKPKLFLVVPPPQRDFTAEPTENGSEAFSGVPSPTGAEGEAGQSQEEKSSPASQAGSHATAPTSGSTVLERGTAGSLSPGVVEANAPMVQPSTSPGPAQEEAGENRVDGERNVKSCASPRGGEAGLPEPNTAGPSSDTADVSKEGGNDEVLTPTRPRTTEDLFAAIHRSKRKVLGRKDSEDDHTRNHSPSPPVTPTGAAPSLASPKQVGSIQRSLKKSSTSSDNFKALLLKKGSRSDTSARMSAAEMLKSTDPRFQRSRSEPSPEVPDSPSSFSPNKNRRAPEEWAKNEGLMPRSLSFSGPRYSRSRTPPSAASSRYSMRNRIQSSPMTVISEGEGEPAEPADNKVRRTLDTTRGCSLDRLAGQETDRGSPLCSEEPASVDGIGRAKDDGPSEQCRGPEQKS

Target ORF information:

RefSeq Version	XM_006222833.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus NHS-like 1 (Nhsl1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006222833.3

ORF Insert Sequence:

ATGTTCTGCC TGAAAGCGGT TTCCAACTTA GACGAGGAGA GCCGATGGAC CGTCCACTAT 
ACGGCGCCGT GGCACCAGCA GGAGAATGTG TTCCTCCCCT CCACAAGACC CCCCTGTGTT 
GAGGACCTGC ACCGCCAAGC GAAGCTCAAC CTCAAATCAG TGCTGAGGGA ATGTGACAAG 
CTGAGGCGCG ATGGCTGCCG AAGTTCTCAG TACTATTCTC AGGGACCCAC CTTTGCAGCC 
AGCTCCAGCC CAGGCGATGA CTATCAAGAG GAAGATGCAG AAACAGACCG AAAGTATTCT 
CTCTCTTCAT CTGAAGAAGA AAGATTTATT GGTATCAGGA GACCTAAAAC GCCAACCTCA 
GGTGACTTCT CTGACCTTCA TACTCAGACA AACTGGACCA AGTCCCTGCC GCTGCCAACA 
CCAGAAGAGA AGACACGACA GCAAGCCCAG ACAGTCCAGG CTGATGTGGT TCCTATTAAC 
ATAACTGGGG AGAATTTTGA TCGTCAGGCC AGCCTTCGGC GGTCTCTAAT TTACACTGAC 
ACTCTGGTAA GACGACCGAA GAAAGTCAAA AGGAGAAAGA CTATTTCAGG AATCCCTGAC 
ATCATTCAGA AGGAGCTAGC GTCAGCCACG GGCCAAGAGG ATGATGGTAG CACCCATTCT 
CTATACTCCC CAGACCACTA CTCTACATTA GGAAGGCTTG ATAGCTATCG GTCCACGGGG 
CAATGCTTGG AAACCAGGGA CTCTGGCTGT CAGACAGAAG AAGTGAAAGT CATCCCACCG 
TCAGTGAGAA GGATCAGGGC TCACAAGGGG CTGGGCATTG CTGCCCAGAT GAGCCACCTC 
TCGGGTTCCT CTGGCAACAT GTCTGTGCTG AGCGACTCGG CTGGTATCGT GTTCCCATCT 
CGCCTCAATA ACGATACTGG TTTCCACAGC CTTCCACGCG CTGGCGCAAG GGCAAGCACG 
TATTCCCTGG AGGCAAGGAT GGGTGCCCTG GGCTCTGCTG AAGATACGGA TGATACTTTT 
CCCTACCGAG GGGGTAGCTC CCAGGGGCCT GAAAACTTTG CACATTTAGG GGGTGCCTCG 
AGTACCGGGA TGCTTTCGAG GCCCAAATCC CAGCAGCTGA GGCTCTTGGA GAGCCCAGCG 
TGTGTGGTTT CACCGCACGC GGCCTACTCT ACCAGCGTCA TCCCAAACGC CACACTGCTG 
TCCTCTTCGG AGGTTATTGT CATCCACACC GCTCAGAGTG CAGGACAGCT GGACAGTAGA 
GTCCCCGGCT CATCCTCATA CTCAAAGATA AAACCCAGAG ACCATCCCAC ACCCAGGTGC 
TCTGCGAGAG ATGAGCGCCA GTCCCCACGA CATCACTGGA ACGAGGGTCA TATCATTCAC 
TCACAGGCTT TAGCCTCCGC TGCCCCCGGT GCCACCGCAC TGTTATCCCT CCGTGACTCA 
GAGGTCTCTC TCAATGCCCC AGCTATCAAT AGGGAGAACG GGTCCCAAGC CATACTTTAT 
CACTGTAGAA GCAACCTGGC CTTTCCTGCC CACCCTCCGG ATGTGGATGG CAAGAGCGAG 
TGCAGTTCTT CAGGGGACAG GGGATGTGGC AGTTCTGAGT CCTGGGAATA CAAAGCCTCC 
AGCAATGGGC GGGCTTCCCC ACTGAAGCCG CACCTTGCGA CTCCCGGTTG CTCTACCCCC 
ACAAGTAACA TGAGCAGCTG CAGCTTGGAC CAAGCATCCC TCAAGGAGGA TGCCAGGTCC 
CTGTATTCAG AGGACCACGA TGGTTACTAT ATGACCGCCC AGGGTGATTC TAGACACGAG 
GCTGGGAGCG CGTACGGTAT CAGTGATGGC TTTGGAAACC CCAGGCACAG CACGGTCAAT 
GTTTTTGACG GAAGGGCTCA GAGAAGCCAA GGGGATCAGG CCACTCAGCA GGATAGAATC 
CTCTCAAGAA ACATCTCTCT GAAGAAAGCA AAAAAACCAC CACCGCCACC ATCCCGGACA 
GATTCTCTGC GAAGGATTCC CAAGAAGAAC AACCAGACAA ATGGGCAGGT GCTCAACGAG 
AGCCTGATCG CCTCACTCCA GCATTCTCTG CAGCTAAGCC TCCCTGGCCA AGGCAGCGGT 
TCCCCTTCCC AGAGCCCCTG CAGTGACTGT GAGGAACCCT GGCTCCCCAG GTCCAGAAGC 
CAGAGCATTG TTAGCGAGGG TAGTAGCTTG ACCTCCACCA CCACTCCCAA CGTGTATTCT 
CTGTGCGGGC TCACTCCATC TCAGAGCGAC ACAAGCAGCG TCAAGTCGGA ATACGCAGAC 
CCCTGGGGCT ACTACATTGA CTACACAACT TTGCAGGGAG ATCCAGGGAA CCCCACAGGG 
GGCTGCCCAG CCAGTAGTGA GGCAGCCACT GGAAATGGGC CAGTGCGCCA CATCCAGGAG 
GGGTCGAGAG CCCCAGTGCC CCAAGTGCCG GGCTGCTCCG TGAAACCAAA GATCACCTCA 
CCGGAGAAGT CACAGAGAGT CACCTCTCCA TCCAGTGGGT ATTCTAGCCA GTCGAATACA 
CCCACAGCAC TCACCCCCGT GCCTGTGTTT TTAAAATCGA TGTCACCAGC AAATGGGAAG 
GGGAAGGCCA AGCCCAAGGT ACCAGAAAGA AAATCGTCTC TGATCTCTTC AGTGTCCATC 
TCCTCATCGT CCACTTCCCT CTCCTCTAAT ACCTCTACGG AAGGAAGTGG GACCGTGAAG 
AAACTGGATT CAGCCCTGGC CTCGGCCCTT GCTCCTCCCC CTCCTCCTCT TCCCGCGCTG 
CCTTCTCCGA GTCTGGCAAA CAAGTCCCCC TTCCTTCCAC CTCCTCCTCC TCTAGCAGAT 
TGCTCCGAGG GCTCTCCCTT ACCGCCCTCT CCTGTGTTCC CCCCTCCACC ACCAGAAGCC 
CTTGTTCCCT TCTGCTCCCC CACTGACAGG TGCCTTTCTC CTTCCCCCAC AGCTTTTAGC 
CCCCCTCTTC CAAGATGCTC ACCTCCCTTG CCAGCACCTC CACCTTTCTT GCCCCCATCA 
GAACCCCCAC CTGCTCCGCC TCTGGACCCC AAATTCATGA AAGAAAACAG GCCCTTTATC 
AAAAACTCTA GCCAGTCCGA ATCCTCTAGG GAGGCCCTCA GGCGGCCTGC CAACAAGGAG 
GAGGGCTGCC GACCCTCGAT GCCCCTGATC ACCACAGAAG CTTTGCAGAT GGTGCAGTTG 
AGGCCAGTGA GGAAGAACTC AGGTGCTGAG ACGGTCCTGT TTTCTGAACC ATCAGCTCAG 
GAACCACGAA CCCCGACTGC TCCACAGTAT CACTTAAAGC CATCTGCTTT CCTCAAATCC 
CGAAATAGCA TAAATGAAAT GGAGAGTGAA AGCCAGGCTG CCTCTGTGAC GAGCTCGCTT 
CCGATGCCTG CCAAGAGCCA GAGTCAGGGT GACCATGACA GTGCAGTCGA GCGTGGCGGC 
CTTCAGAGCT GCAGCGATGG AGCTCCAGGC CCGGGTCCCA GCCTCAGGAC CGCTCTGCTC 
CCAGACTCTT CACCCAGCAG GAAGCCACCC CCCATCTCCA AGAAGCCCAA ACTGTTCCTG 
GTGGTACCAC CTCCGCAGAG AGATTTCACA GCGGAGCCCA CAGAGAACGG GAGCGAAGCT 
TTCTCAGGCG TGCCCAGCCC TACCGGGGCA GAGGGGGAAG CTGGGCAGAG CCAAGAGGAG 
AAATCCAGCC CAGCATCCCA AGCTGGCTCT CATGCTACGG CCCCCACCTC CGGCAGTACG 
GTTCTAGAAA GAGGAACTGC AGGGTCCTTG TCCCCTGGCG TTGTGGAAGC TAATGCCCCC 
ATGGTCCAAC CTAGTACATC ACCGGGCCCC GCACAGGAAG AGGCAGGAGA GAACCGCGTG 
GATGGTGAGA GAAATGTGAA GAGCTGCGCG TCTCCACGGG GCGGAGAAGC TGGGTTGCCG 
GAACCCAACA CAGCTGGTCC GTCTTCAGAC ACGGCAGACG TTTCTAAAGA AGGAGGGAAC 
GATGAGGTGC TGACCCCTAC CAGACCCAGG ACAACGGAAG ACCTGTTTGC AGCCATTCAC 
AGATCCAAAA GGAAAGTCCT TGGTCGGAAG GACTCCGAAG ATGATCACAC TCGAAATCAT 
TCTCCCTCCC CGCCTGTGAC ACCCACAGGT GCTGCCCCTA GCCTGGCCTC TCCAAAGCAA 
GTAGGGTCCA TCCAGCGAAG CCTAAAAAAG AGCAGTACCA GCAGCGACAA CTTCAAAGCT 
CTGCTGCTCA AGAAGGGGAG TCGCTCGGAC ACCAGCGCTC GCATGTCCGC GGCGGAGATG 
CTCAAGTCCA CGGACCCTCG ATTCCAGAGA TCTAGGTCGG AGCCTTCGCC AGAGGTCCCG 
GACAGCCCAT CCAGCTTCTC TCCCAATAAG AACAGGAGAG CTCCGGAGGA GTGGGCCAAG 
AATGAAGGAC TGATGCCTCG GAGTCTGTCC TTTTCCGGCC CTCGGTACAG CCGCAGCAGG 
ACGCCACCCT CTGCGGCCAG CAGCAGGTAC AGCATGAGGA ACCGCATCCA AAGCAGCCCC 
ATGACAGTCA TCTCTGAGGG AGAGGGAGAG CCTGCCGAGC CCGCAGACAA CAAAGTACGC 
AGGACCCTGG ACACTACGAG GGGATGCTCT CTGGACAGAC TGGCGGGACA GGAGACGGAC 
CGGGGCAGCC CGCTGTGTAG CGAGGAGCCT GCCTCTGTGG ATGGGATAGG AAGGGCTAAG 
GACGACGGTC CCTCGGAGCA GTGTAGGGGT CCTGAACAGA AGAGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nhsl1 ( XM_006227670.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006227732.1 Rattus norvegicus NHS-like 1 (Nhsl1), transcript variant X6, mRNA.

Price & Availability↑

CloneID	ORa13979
Clone ID Related Accession (Same CDS sequence)	XM_006222833.3 , XM_006227670.3
Accession Version	XM_006227670.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4728bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	NHS-like protein 1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006227670.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCTGCC TGAAAGCGGT TTCCAACTTA GACGAGGAGA GCCGATGGAC CGTCCACTAT 
ACGGCGCCGT GGCACCAGCA GGAGAATGTG TTCCTCCCCT CCACAAGACC CCCCTGTGTT 
GAGGACCTGC ACCGCCAAGC GAAGCTCAAC CTCAAATCAG TGCTGAGGGA ATGTGACAAG 
CTGAGGCGCG ATGGCTGCCG AAGTTCTCAG TACTATTCTC AGGGACCCAC CTTTGCAGCC 
AGCTCCAGCC CAGGCGATGA CTATCAAGAG GAAGATGCAG AAACAGACCG AAAGTATTCT 
CTCTCTTCAT CTGAAGAAGA AAGATTTATT GGTATCAGGA GACCTAAAAC GCCAACCTCA 
GGTGACTTCT CTGACCTTCA TACTCAGACA AACTGGACCA AGTCCCTGCC GCTGCCAACA 
CCAGAAGAGA AGACACGACA GCAAGCCCAG ACAGTCCAGG CTGATGTGGT TCCTATTAAC 
ATAACTGGGG AGAATTTTGA TCGTCAGGCC AGCCTTCGGC GGTCTCTAAT TTACACTGAC 
ACTCTGGTAA GACGACCGAA GAAAGTCAAA AGGAGAAAGA CTATTTCAGG AATCCCTGAC 
ATCATTCAGA AGGAGCTAGC GTCAGCCACG GGCCAAGAGG ATGATGGTAG CACCCATTCT 
CTATACTCCC CAGACCACTA CTCTACATTA GGAAGGCTTG ATAGCTATCG GTCCACGGGG 
CAATGCTTGG AAACCAGGGA CTCTGGCTGT CAGACAGAAG AAGTGAAAGT CATCCCACCG 
TCAGTGAGAA GGATCAGGGC TCACAAGGGG CTGGGCATTG CTGCCCAGAT GAGCCACCTC 
TCGGGTTCCT CTGGCAACAT GTCTGTGCTG AGCGACTCGG CTGGTATCGT GTTCCCATCT 
CGCCTCAATA ACGATACTGG TTTCCACAGC CTTCCACGCG CTGGCGCAAG GGCAAGCACG 
TATTCCCTGG AGGCAAGGAT GGGTGCCCTG GGCTCTGCTG AAGATACGGA TGATACTTTT 
CCCTACCGAG GGGGTAGCTC CCAGGGGCCT GAAAACTTTG CACATTTAGG GGGTGCCTCG 
AGTACCGGGA TGCTTTCGAG GCCCAAATCC CAGCAGCTGA GGCTCTTGGA GAGCCCAGCG 
TGTGTGGTTT CACCGCACGC GGCCTACTCT ACCAGCGTCA TCCCAAACGC CACACTGCTG 
TCCTCTTCGG AGGTTATTGT CATCCACACC GCTCAGAGTG CAGGACAGCT GGACAGTAGA 
GTCCCCGGCT CATCCTCATA CTCAAAGATA AAACCCAGAG ACCATCCCAC ACCCAGGTGC 
TCTGCGAGAG ATGAGCGCCA GTCCCCACGA CATCACTGGA ACGAGGGTCA TATCATTCAC 
TCACAGGCTT TAGCCTCCGC TGCCCCCGGT GCCACCGCAC TGTTATCCCT CCGTGACTCA 
GAGGTCTCTC TCAATGCCCC AGCTATCAAT AGGGAGAACG GGTCCCAAGC CATACTTTAT 
CACTGTAGAA GCAACCTGGC CTTTCCTGCC CACCCTCCGG ATGTGGATGG CAAGAGCGAG 
TGCAGTTCTT CAGGGGACAG GGGATGTGGC AGTTCTGAGT CCTGGGAATA CAAAGCCTCC 
AGCAATGGGC GGGCTTCCCC ACTGAAGCCG CACCTTGCGA CTCCCGGTTG CTCTACCCCC 
ACAAGTAACA TGAGCAGCTG CAGCTTGGAC CAAGCATCCC TCAAGGAGGA TGCCAGGTCC 
CTGTATTCAG AGGACCACGA TGGTTACTAT ATGACCGCCC AGGGTGATTC TAGACACGAG 
GCTGGGAGCG CGTACGGTAT CAGTGATGGC TTTGGAAACC CCAGGCACAG CACGGTCAAT 
GTTTTTGACG GAAGGGCTCA GAGAAGCCAA GGGGATCAGG CCACTCAGCA GGATAGAATC 
CTCTCAAGAA ACATCTCTCT GAAGAAAGCA AAAAAACCAC CACCGCCACC ATCCCGGACA 
GATTCTCTGC GAAGGATTCC CAAGAAGAAC AACCAGACAA ATGGGCAGGT GCTCAACGAG 
AGCCTGATCG CCTCACTCCA GCATTCTCTG CAGCTAAGCC TCCCTGGCCA AGGCAGCGGT 
TCCCCTTCCC AGAGCCCCTG CAGTGACTGT GAGGAACCCT GGCTCCCCAG GTCCAGAAGC 
CAGAGCATTG TTAGCGAGGG TAGTAGCTTG ACCTCCACCA CCACTCCCAA CGTGTATTCT 
CTGTGCGGGC TCACTCCATC TCAGAGCGAC ACAAGCAGCG TCAAGTCGGA ATACGCAGAC 
CCCTGGGGCT ACTACATTGA CTACACAACT TTGCAGGGAG ATCCAGGGAA CCCCACAGGG 
GGCTGCCCAG CCAGTAGTGA GGCAGCCACT GGAAATGGGC CAGTGCGCCA CATCCAGGAG 
GGGTCGAGAG CCCCAGTGCC CCAAGTGCCG GGCTGCTCCG TGAAACCAAA GATCACCTCA 
CCGGAGAAGT CACAGAGAGT CACCTCTCCA TCCAGTGGGT ATTCTAGCCA GTCGAATACA 
CCCACAGCAC TCACCCCCGT GCCTGTGTTT TTAAAATCGA TGTCACCAGC AAATGGGAAG 
GGGAAGGCCA AGCCCAAGGT ACCAGAAAGA AAATCGTCTC TGATCTCTTC AGTGTCCATC 
TCCTCATCGT CCACTTCCCT CTCCTCTAAT ACCTCTACGG AAGGAAGTGG GACCGTGAAG 
AAACTGGATT CAGCCCTGGC CTCGGCCCTT GCTCCTCCCC CTCCTCCTCT TCCCGCGCTG 
CCTTCTCCGA GTCTGGCAAA CAAGTCCCCC TTCCTTCCAC CTCCTCCTCC TCTAGCAGAT 
TGCTCCGAGG GCTCTCCCTT ACCGCCCTCT CCTGTGTTCC CCCCTCCACC ACCAGAAGCC 
CTTGTTCCCT TCTGCTCCCC CACTGACAGG TGCCTTTCTC CTTCCCCCAC AGCTTTTAGC 
CCCCCTCTTC CAAGATGCTC ACCTCCCTTG CCAGCACCTC CACCTTTCTT GCCCCCATCA 
GAACCCCCAC CTGCTCCGCC TCTGGACCCC AAATTCATGA AAGAAAACAG GCCCTTTATC 
AAAAACTCTA GCCAGTCCGA ATCCTCTAGG GAGGCCCTCA GGCGGCCTGC CAACAAGGAG 
GAGGGCTGCC GACCCTCGAT GCCCCTGATC ACCACAGAAG CTTTGCAGAT GGTGCAGTTG 
AGGCCAGTGA GGAAGAACTC AGGTGCTGAG ACGGTCCTGT TTTCTGAACC ATCAGCTCAG 
GAACCACGAA CCCCGACTGC TCCACAGTAT CACTTAAAGC CATCTGCTTT CCTCAAATCC 
CGAAATAGCA TAAATGAAAT GGAGAGTGAA AGCCAGGCTG CCTCTGTGAC GAGCTCGCTT 
CCGATGCCTG CCAAGAGCCA GAGTCAGGGT GACCATGACA GTGCAGTCGA GCGTGGCGGC 
CTTCAGAGCT GCAGCGATGG AGCTCCAGGC CCGGGTCCCA GCCTCAGGAC CGCTCTGCTC 
CCAGACTCTT CACCCAGCAG GAAGCCACCC CCCATCTCCA AGAAGCCCAA ACTGTTCCTG 
GTGGTACCAC CTCCGCAGAG AGATTTCACA GCGGAGCCCA CAGAGAACGG GAGCGAAGCT 
TTCTCAGGCG TGCCCAGCCC TACCGGGGCA GAGGGGGAAG CTGGGCAGAG CCAAGAGGAG 
AAATCCAGCC CAGCATCCCA AGCTGGCTCT CATGCTACGG CCCCCACCTC CGGCAGTACG 
GTTCTAGAAA GAGGAACTGC AGGGTCCTTG TCCCCTGGCG TTGTGGAAGC TAATGCCCCC 
ATGGTCCAAC CTAGTACATC ACCGGGCCCC GCACAGGAAG AGGCAGGAGA GAACCGCGTG 
GATGGTGAGA GAAATGTGAA GAGCTGCGCG TCTCCACGGG GCGGAGAAGC TGGGTTGCCG 
GAACCCAACA CAGCTGGTCC GTCTTCAGAC ACGGCAGACG TTTCTAAAGA AGGAGGGAAC 
GATGAGGTGC TGACCCCTAC CAGACCCAGG ACAACGGAAG ACCTGTTTGC AGCCATTCAC 
AGATCCAAAA GGAAAGTCCT TGGTCGGAAG GACTCCGAAG ATGATCACAC TCGAAATCAT 
TCTCCCTCCC CGCCTGTGAC ACCCACAGGT GCTGCCCCTA GCCTGGCCTC TCCAAAGCAA 
GTAGGGTCCA TCCAGCGAAG CCTAAAAAAG AGCAGTACCA GCAGCGACAA CTTCAAAGCT 
CTGCTGCTCA AGAAGGGGAG TCGCTCGGAC ACCAGCGCTC GCATGTCCGC GGCGGAGATG 
CTCAAGTCCA CGGACCCTCG ATTCCAGAGA TCTAGGTCGG AGCCTTCGCC AGAGGTCCCG 
GACAGCCCAT CCAGCTTCTC TCCCAATAAG AACAGGAGAG CTCCGGAGGA GTGGGCCAAG 
AATGAAGGAC TGATGCCTCG GAGTCTGTCC TTTTCCGGCC CTCGGTACAG CCGCAGCAGG 
ACGCCACCCT CTGCGGCCAG CAGCAGGTAC AGCATGAGGA ACCGCATCCA AAGCAGCCCC 
ATGACAGTCA TCTCTGAGGG AGAGGGAGAG CCTGCCGAGC CCGCAGACAA CAAAGTACGC 
AGGACCCTGG ACACTACGAG GGGATGCTCT CTGGACAGAC TGGCGGGACA GGAGACGGAC 
CGGGGCAGCC CGCTGTGTAG CGAGGAGCCT GCCTCTGTGG ATGGGATAGG AAGGGCTAAG 
GACGACGGTC CCTCGGAGCA GTGTAGGGGT CCTGAACAGA AGAGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006227732.1
CDS	882..5609
Translation	MFCLKAVSNLDEESRWTVHYTAPWHQQENVFLPSTRPPCVEDLHRQAKLNLKSVLRECDKLRRDGCRSSQYYSQGPTFAASSSPGDDYQEEDAETDRKYSLSSSEEERFIGIRRPKTPTSGDFSDLHTQTNWTKSLPLPTPEEKTRQQAQTVQADVVPINITGENFDRQASLRRSLIYTDTLVRRPKKVKRRKTISGIPDIIQKELASATGQEDDGSTHSLYSPDHYSTLGRLDSYRSTGQCLETRDSGCQTEEVKVIPPSVRRIRAHKGLGIAAQMSHLSGSSGNMSVLSDSAGIVFPSRLNNDTGFHSLPRAGARASTYSLEARMGALGSAEDTDDTFPYRGGSSQGPENFAHLGGASSTGMLSRPKSQQLRLLESPACVVSPHAAYSTSVIPNATLLSSSEVIVIHTAQSAGQLDSRVPGSSSYSKIKPRDHPTPRCSARDERQSPRHHWNEGHIIHSQALASAAPGATALLSLRDSEVSLNAPAINRENGSQAILYHCRSNLAFPAHPPDVDGKSECSSSGDRGCGSSESWEYKASSNGRASPLKPHLATPGCSTPTSNMSSCSLDQASLKEDARSLYSEDHDGYYMTAQGDSRHEAGSAYGISDGFGNPRHSTVNVFDGRAQRSQGDQATQQDRILSRNISLKKAKKPPPPPSRTDSLRRIPKKNNQTNGQVLNESLIASLQHSLQLSLPGQGSGSPSQSPCSDCEEPWLPRSRSQSIVSEGSSLTSTTTPNVYSLCGLTPSQSDTSSVKSEYADPWGYYIDYTTLQGDPGNPTGGCPASSEAATGNGPVRHIQEGSRAPVPQVPGCSVKPKITSPEKSQRVTSPSSGYSSQSNTPTALTPVPVFLKSMSPANGKGKAKPKVPERKSSLISSVSISSSSTSLSSNTSTEGSGTVKKLDSALASALAPPPPPLPALPSPSLANKSPFLPPPPPLADCSEGSPLPPSPVFPPPPPEALVPFCSPTDRCLSPSPTAFSPPLPRCSPPLPAPPPFLPPSEPPPAPPLDPKFMKENRPFIKNSSQSESSREALRRPANKEEGCRPSMPLITTEALQMVQLRPVRKNSGAETVLFSEPSAQEPRTPTAPQYHLKPSAFLKSRNSINEMESESQAASVTSSLPMPAKSQSQGDHDSAVERGGLQSCSDGAPGPGPSLRTALLPDSSPSRKPPPISKKPKLFLVVPPPQRDFTAEPTENGSEAFSGVPSPTGAEGEAGQSQEEKSSPASQAGSHATAPTSGSTVLERGTAGSLSPGVVEANAPMVQPSTSPGPAQEEAGENRVDGERNVKSCASPRGGEAGLPEPNTAGPSSDTADVSKEGGNDEVLTPTRPRTTEDLFAAIHRSKRKVLGRKDSEDDHTRNHSPSPPVTPTGAAPSLASPKQVGSIQRSLKKSSTSSDNFKALLLKKGSRSDTSARMSAAEMLKSTDPRFQRSRSEPSPEVPDSPSSFSPNKNRRAPEEWAKNEGLMPRSLSFSGPRYSRSRTPPSAASSRYSMRNRIQSSPMTVISEGEGEPAEPADNKVRRTLDTTRGCSLDRLAGQETDRGSPLCSEEPASVDGIGRAKDDGPSEQCRGPEQKS

Target ORF information:

RefSeq Version	XM_006227670.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus NHS-like 1 (Nhsl1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006227670.3

ORF Insert Sequence:

ATGTTCTGCC TGAAAGCGGT TTCCAACTTA GACGAGGAGA GCCGATGGAC CGTCCACTAT 
ACGGCGCCGT GGCACCAGCA GGAGAATGTG TTCCTCCCCT CCACAAGACC CCCCTGTGTT 
GAGGACCTGC ACCGCCAAGC GAAGCTCAAC CTCAAATCAG TGCTGAGGGA ATGTGACAAG 
CTGAGGCGCG ATGGCTGCCG AAGTTCTCAG TACTATTCTC AGGGACCCAC CTTTGCAGCC 
AGCTCCAGCC CAGGCGATGA CTATCAAGAG GAAGATGCAG AAACAGACCG AAAGTATTCT 
CTCTCTTCAT CTGAAGAAGA AAGATTTATT GGTATCAGGA GACCTAAAAC GCCAACCTCA 
GGTGACTTCT CTGACCTTCA TACTCAGACA AACTGGACCA AGTCCCTGCC GCTGCCAACA 
CCAGAAGAGA AGACACGACA GCAAGCCCAG ACAGTCCAGG CTGATGTGGT TCCTATTAAC 
ATAACTGGGG AGAATTTTGA TCGTCAGGCC AGCCTTCGGC GGTCTCTAAT TTACACTGAC 
ACTCTGGTAA GACGACCGAA GAAAGTCAAA AGGAGAAAGA CTATTTCAGG AATCCCTGAC 
ATCATTCAGA AGGAGCTAGC GTCAGCCACG GGCCAAGAGG ATGATGGTAG CACCCATTCT 
CTATACTCCC CAGACCACTA CTCTACATTA GGAAGGCTTG ATAGCTATCG GTCCACGGGG 
CAATGCTTGG AAACCAGGGA CTCTGGCTGT CAGACAGAAG AAGTGAAAGT CATCCCACCG 
TCAGTGAGAA GGATCAGGGC TCACAAGGGG CTGGGCATTG CTGCCCAGAT GAGCCACCTC 
TCGGGTTCCT CTGGCAACAT GTCTGTGCTG AGCGACTCGG CTGGTATCGT GTTCCCATCT 
CGCCTCAATA ACGATACTGG TTTCCACAGC CTTCCACGCG CTGGCGCAAG GGCAAGCACG 
TATTCCCTGG AGGCAAGGAT GGGTGCCCTG GGCTCTGCTG AAGATACGGA TGATACTTTT 
CCCTACCGAG GGGGTAGCTC CCAGGGGCCT GAAAACTTTG CACATTTAGG GGGTGCCTCG 
AGTACCGGGA TGCTTTCGAG GCCCAAATCC CAGCAGCTGA GGCTCTTGGA GAGCCCAGCG 
TGTGTGGTTT CACCGCACGC GGCCTACTCT ACCAGCGTCA TCCCAAACGC CACACTGCTG 
TCCTCTTCGG AGGTTATTGT CATCCACACC GCTCAGAGTG CAGGACAGCT GGACAGTAGA 
GTCCCCGGCT CATCCTCATA CTCAAAGATA AAACCCAGAG ACCATCCCAC ACCCAGGTGC 
TCTGCGAGAG ATGAGCGCCA GTCCCCACGA CATCACTGGA ACGAGGGTCA TATCATTCAC 
TCACAGGCTT TAGCCTCCGC TGCCCCCGGT GCCACCGCAC TGTTATCCCT CCGTGACTCA 
GAGGTCTCTC TCAATGCCCC AGCTATCAAT AGGGAGAACG GGTCCCAAGC CATACTTTAT 
CACTGTAGAA GCAACCTGGC CTTTCCTGCC CACCCTCCGG ATGTGGATGG CAAGAGCGAG 
TGCAGTTCTT CAGGGGACAG GGGATGTGGC AGTTCTGAGT CCTGGGAATA CAAAGCCTCC 
AGCAATGGGC GGGCTTCCCC ACTGAAGCCG CACCTTGCGA CTCCCGGTTG CTCTACCCCC 
ACAAGTAACA TGAGCAGCTG CAGCTTGGAC CAAGCATCCC TCAAGGAGGA TGCCAGGTCC 
CTGTATTCAG AGGACCACGA TGGTTACTAT ATGACCGCCC AGGGTGATTC TAGACACGAG 
GCTGGGAGCG CGTACGGTAT CAGTGATGGC TTTGGAAACC CCAGGCACAG CACGGTCAAT 
GTTTTTGACG GAAGGGCTCA GAGAAGCCAA GGGGATCAGG CCACTCAGCA GGATAGAATC 
CTCTCAAGAA ACATCTCTCT GAAGAAAGCA AAAAAACCAC CACCGCCACC ATCCCGGACA 
GATTCTCTGC GAAGGATTCC CAAGAAGAAC AACCAGACAA ATGGGCAGGT GCTCAACGAG 
AGCCTGATCG CCTCACTCCA GCATTCTCTG CAGCTAAGCC TCCCTGGCCA AGGCAGCGGT 
TCCCCTTCCC AGAGCCCCTG CAGTGACTGT GAGGAACCCT GGCTCCCCAG GTCCAGAAGC 
CAGAGCATTG TTAGCGAGGG TAGTAGCTTG ACCTCCACCA CCACTCCCAA CGTGTATTCT 
CTGTGCGGGC TCACTCCATC TCAGAGCGAC ACAAGCAGCG TCAAGTCGGA ATACGCAGAC 
CCCTGGGGCT ACTACATTGA CTACACAACT TTGCAGGGAG ATCCAGGGAA CCCCACAGGG 
GGCTGCCCAG CCAGTAGTGA GGCAGCCACT GGAAATGGGC CAGTGCGCCA CATCCAGGAG 
GGGTCGAGAG CCCCAGTGCC CCAAGTGCCG GGCTGCTCCG TGAAACCAAA GATCACCTCA 
CCGGAGAAGT CACAGAGAGT CACCTCTCCA TCCAGTGGGT ATTCTAGCCA GTCGAATACA 
CCCACAGCAC TCACCCCCGT GCCTGTGTTT TTAAAATCGA TGTCACCAGC AAATGGGAAG 
GGGAAGGCCA AGCCCAAGGT ACCAGAAAGA AAATCGTCTC TGATCTCTTC AGTGTCCATC 
TCCTCATCGT CCACTTCCCT CTCCTCTAAT ACCTCTACGG AAGGAAGTGG GACCGTGAAG 
AAACTGGATT CAGCCCTGGC CTCGGCCCTT GCTCCTCCCC CTCCTCCTCT TCCCGCGCTG 
CCTTCTCCGA GTCTGGCAAA CAAGTCCCCC TTCCTTCCAC CTCCTCCTCC TCTAGCAGAT 
TGCTCCGAGG GCTCTCCCTT ACCGCCCTCT CCTGTGTTCC CCCCTCCACC ACCAGAAGCC 
CTTGTTCCCT TCTGCTCCCC CACTGACAGG TGCCTTTCTC CTTCCCCCAC AGCTTTTAGC 
CCCCCTCTTC CAAGATGCTC ACCTCCCTTG CCAGCACCTC CACCTTTCTT GCCCCCATCA 
GAACCCCCAC CTGCTCCGCC TCTGGACCCC AAATTCATGA AAGAAAACAG GCCCTTTATC 
AAAAACTCTA GCCAGTCCGA ATCCTCTAGG GAGGCCCTCA GGCGGCCTGC CAACAAGGAG 
GAGGGCTGCC GACCCTCGAT GCCCCTGATC ACCACAGAAG CTTTGCAGAT GGTGCAGTTG 
AGGCCAGTGA GGAAGAACTC AGGTGCTGAG ACGGTCCTGT TTTCTGAACC ATCAGCTCAG 
GAACCACGAA CCCCGACTGC TCCACAGTAT CACTTAAAGC CATCTGCTTT CCTCAAATCC 
CGAAATAGCA TAAATGAAAT GGAGAGTGAA AGCCAGGCTG CCTCTGTGAC GAGCTCGCTT 
CCGATGCCTG CCAAGAGCCA GAGTCAGGGT GACCATGACA GTGCAGTCGA GCGTGGCGGC 
CTTCAGAGCT GCAGCGATGG AGCTCCAGGC CCGGGTCCCA GCCTCAGGAC CGCTCTGCTC 
CCAGACTCTT CACCCAGCAG GAAGCCACCC CCCATCTCCA AGAAGCCCAA ACTGTTCCTG 
GTGGTACCAC CTCCGCAGAG AGATTTCACA GCGGAGCCCA CAGAGAACGG GAGCGAAGCT 
TTCTCAGGCG TGCCCAGCCC TACCGGGGCA GAGGGGGAAG CTGGGCAGAG CCAAGAGGAG 
AAATCCAGCC CAGCATCCCA AGCTGGCTCT CATGCTACGG CCCCCACCTC CGGCAGTACG 
GTTCTAGAAA GAGGAACTGC AGGGTCCTTG TCCCCTGGCG TTGTGGAAGC TAATGCCCCC 
ATGGTCCAAC CTAGTACATC ACCGGGCCCC GCACAGGAAG AGGCAGGAGA GAACCGCGTG 
GATGGTGAGA GAAATGTGAA GAGCTGCGCG TCTCCACGGG GCGGAGAAGC TGGGTTGCCG 
GAACCCAACA CAGCTGGTCC GTCTTCAGAC ACGGCAGACG TTTCTAAAGA AGGAGGGAAC 
GATGAGGTGC TGACCCCTAC CAGACCCAGG ACAACGGAAG ACCTGTTTGC AGCCATTCAC 
AGATCCAAAA GGAAAGTCCT TGGTCGGAAG GACTCCGAAG ATGATCACAC TCGAAATCAT 
TCTCCCTCCC CGCCTGTGAC ACCCACAGGT GCTGCCCCTA GCCTGGCCTC TCCAAAGCAA 
GTAGGGTCCA TCCAGCGAAG CCTAAAAAAG AGCAGTACCA GCAGCGACAA CTTCAAAGCT 
CTGCTGCTCA AGAAGGGGAG TCGCTCGGAC ACCAGCGCTC GCATGTCCGC GGCGGAGATG 
CTCAAGTCCA CGGACCCTCG ATTCCAGAGA TCTAGGTCGG AGCCTTCGCC AGAGGTCCCG 
GACAGCCCAT CCAGCTTCTC TCCCAATAAG AACAGGAGAG CTCCGGAGGA GTGGGCCAAG 
AATGAAGGAC TGATGCCTCG GAGTCTGTCC TTTTCCGGCC CTCGGTACAG CCGCAGCAGG 
ACGCCACCCT CTGCGGCCAG CAGCAGGTAC AGCATGAGGA ACCGCATCCA AAGCAGCCCC 
ATGACAGTCA TCTCTGAGGG AGAGGGAGAG CCTGCCGAGC CCGCAGACAA CAAAGTACGC 
AGGACCCTGG ACACTACGAG GGGATGCTCT CTGGACAGAC TGGCGGGACA GGAGACGGAC 
CGGGGCAGCC CGCTGTGTAG CGAGGAGCCT GCCTCTGTGG ATGGGATAGG AAGGGCTAAG 
GACGACGGTC CCTCGGAGCA GTGTAGGGGT CCTGAACAGA AGAGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nhsl1 ( XM_006227671.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006227733.1 Rattus norvegicus NHS-like 1 (Nhsl1), transcript variant X7, mRNA.

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CloneID	ORa13980
Clone ID Related Accession (Same CDS sequence)	XM_006227671.3 , XM_006222834.3
Accession Version	XM_006227671.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4719bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	NHS-like protein 1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006227671.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATACGG TTTCCAACTT AGACGAGGAG AGCCGATGGA CCGTCCACTA TACGGCGCCG 
TGGCACCAGC AGGAGAATGT GTTCCTCCCC TCCACAAGAC CCCCCTGTGT TGAGGACCTG 
CACCGCCAAG CGAAGCTCAA CCTCAAATCA GTGCTGAGGG AATGTGACAA GCTGAGGCGC 
GATGGCTGCC GAAGTTCTCA GTACTATTCT CAGGGACCCA CCTTTGCAGC CAGCTCCAGC 
CCAGGCGATG ACTATCAAGA GGAAGATGCA GAAACAGACC GAAAGTATTC TCTCTCTTCA 
TCTGAAGAAG AAAGATTTAT TGGTATCAGG AGACCTAAAA CGCCAACCTC AGGTGACTTC 
TCTGACCTTC ATACTCAGAC AAACTGGACC AAGTCCCTGC CGCTGCCAAC ACCAGAAGAG 
AAGACACGAC AGCAAGCCCA GACAGTCCAG GCTGATGTGG TTCCTATTAA CATAACTGGG 
GAGAATTTTG ATCGTCAGGC CAGCCTTCGG CGGTCTCTAA TTTACACTGA CACTCTGGTA 
AGACGACCGA AGAAAGTCAA AAGGAGAAAG ACTATTTCAG GAATCCCTGA CATCATTCAG 
AAGGAGCTAG CGTCAGCCAC GGGCCAAGAG GATGATGGTA GCACCCATTC TCTATACTCC 
CCAGACCACT ACTCTACATT AGGAAGGCTT GATAGCTATC GGTCCACGGG GCAATGCTTG 
GAAACCAGGG ACTCTGGCTG TCAGACAGAA GAAGTGAAAG TCATCCCACC GTCAGTGAGA 
AGGATCAGGG CTCACAAGGG GCTGGGCATT GCTGCCCAGA TGAGCCACCT CTCGGGTTCC 
TCTGGCAACA TGTCTGTGCT GAGCGACTCG GCTGGTATCG TGTTCCCATC TCGCCTCAAT 
AACGATACTG GTTTCCACAG CCTTCCACGC GCTGGCGCAA GGGCAAGCAC GTATTCCCTG 
GAGGCAAGGA TGGGTGCCCT GGGCTCTGCT GAAGATACGG ATGATACTTT TCCCTACCGA 
GGGGGTAGCT CCCAGGGGCC TGAAAACTTT GCACATTTAG GGGGTGCCTC GAGTACCGGG 
ATGCTTTCGA GGCCCAAATC CCAGCAGCTG AGGCTCTTGG AGAGCCCAGC GTGTGTGGTT 
TCACCGCACG CGGCCTACTC TACCAGCGTC ATCCCAAACG CCACACTGCT GTCCTCTTCG 
GAGGTTATTG TCATCCACAC CGCTCAGAGT GCAGGACAGC TGGACAGTAG AGTCCCCGGC 
TCATCCTCAT ACTCAAAGAT AAAACCCAGA GACCATCCCA CACCCAGGTG CTCTGCGAGA 
GATGAGCGCC AGTCCCCACG ACATCACTGG AACGAGGGTC ATATCATTCA CTCACAGGCT 
TTAGCCTCCG CTGCCCCCGG TGCCACCGCA CTGTTATCCC TCCGTGACTC AGAGGTCTCT 
CTCAATGCCC CAGCTATCAA TAGGGAGAAC GGGTCCCAAG CCATACTTTA TCACTGTAGA 
AGCAACCTGG CCTTTCCTGC CCACCCTCCG GATGTGGATG GCAAGAGCGA GTGCAGTTCT 
TCAGGGGACA GGGGATGTGG CAGTTCTGAG TCCTGGGAAT ACAAAGCCTC CAGCAATGGG 
CGGGCTTCCC CACTGAAGCC GCACCTTGCG ACTCCCGGTT GCTCTACCCC CACAAGTAAC 
ATGAGCAGCT GCAGCTTGGA CCAAGCATCC CTCAAGGAGG ATGCCAGGTC CCTGTATTCA 
GAGGACCACG ATGGTTACTA TATGACCGCC CAGGGTGATT CTAGACACGA GGCTGGGAGC 
GCGTACGGTA TCAGTGATGG CTTTGGAAAC CCCAGGCACA GCACGGTCAA TGTTTTTGAC 
GGAAGGGCTC AGAGAAGCCA AGGGGATCAG GCCACTCAGC AGGATAGAAT CCTCTCAAGA 
AACATCTCTC TGAAGAAAGC AAAAAAACCA CCACCGCCAC CATCCCGGAC AGATTCTCTG 
CGAAGGATTC CCAAGAAGAA CAACCAGACA AATGGGCAGG TGCTCAACGA GAGCCTGATC 
GCCTCACTCC AGCATTCTCT GCAGCTAAGC CTCCCTGGCC AAGGCAGCGG TTCCCCTTCC 
CAGAGCCCCT GCAGTGACTG TGAGGAACCC TGGCTCCCCA GGTCCAGAAG CCAGAGCATT 
GTTAGCGAGG GTAGTAGCTT GACCTCCACC ACCACTCCCA ACGTGTATTC TCTGTGCGGG 
CTCACTCCAT CTCAGAGCGA CACAAGCAGC GTCAAGTCGG AATACGCAGA CCCCTGGGGC 
TACTACATTG ACTACACAAC TTTGCAGGGA GATCCAGGGA ACCCCACAGG GGGCTGCCCA 
GCCAGTAGTG AGGCAGCCAC TGGAAATGGG CCAGTGCGCC ACATCCAGGA GGGGTCGAGA 
GCCCCAGTGC CCCAAGTGCC GGGCTGCTCC GTGAAACCAA AGATCACCTC ACCGGAGAAG 
TCACAGAGAG TCACCTCTCC ATCCAGTGGG TATTCTAGCC AGTCGAATAC ACCCACAGCA 
CTCACCCCCG TGCCTGTGTT TTTAAAATCG ATGTCACCAG CAAATGGGAA GGGGAAGGCC 
AAGCCCAAGG TACCAGAAAG AAAATCGTCT CTGATCTCTT CAGTGTCCAT CTCCTCATCG 
TCCACTTCCC TCTCCTCTAA TACCTCTACG GAAGGAAGTG GGACCGTGAA GAAACTGGAT 
TCAGCCCTGG CCTCGGCCCT TGCTCCTCCC CCTCCTCCTC TTCCCGCGCT GCCTTCTCCG 
AGTCTGGCAA ACAAGTCCCC CTTCCTTCCA CCTCCTCCTC CTCTAGCAGA TTGCTCCGAG 
GGCTCTCCCT TACCGCCCTC TCCTGTGTTC CCCCCTCCAC CACCAGAAGC CCTTGTTCCC 
TTCTGCTCCC CCACTGACAG GTGCCTTTCT CCTTCCCCCA CAGCTTTTAG CCCCCCTCTT 
CCAAGATGCT CACCTCCCTT GCCAGCACCT CCACCTTTCT TGCCCCCATC AGAACCCCCA 
CCTGCTCCGC CTCTGGACCC CAAATTCATG AAAGAAAACA GGCCCTTTAT CAAAAACTCT 
AGCCAGTCCG AATCCTCTAG GGAGGCCCTC AGGCGGCCTG CCAACAAGGA GGAGGGCTGC 
CGACCCTCGA TGCCCCTGAT CACCACAGAA GCTTTGCAGA TGGTGCAGTT GAGGCCAGTG 
AGGAAGAACT CAGGTGCTGA GACGGTCCTG TTTTCTGAAC CATCAGCTCA GGAACCACGA 
ACCCCGACTG CTCCACAGTA TCACTTAAAG CCATCTGCTT TCCTCAAATC CCGAAATAGC 
ATAAATGAAA TGGAGAGTGA AAGCCAGGCT GCCTCTGTGA CGAGCTCGCT TCCGATGCCT 
GCCAAGAGCC AGAGTCAGGG TGACCATGAC AGTGCAGTCG AGCGTGGCGG CCTTCAGAGC 
TGCAGCGATG GAGCTCCAGG CCCGGGTCCC AGCCTCAGGA CCGCTCTGCT CCCAGACTCT 
TCACCCAGCA GGAAGCCACC CCCCATCTCC AAGAAGCCCA AACTGTTCCT GGTGGTACCA 
CCTCCGCAGA GAGATTTCAC AGCGGAGCCC ACAGAGAACG GGAGCGAAGC TTTCTCAGGC 
GTGCCCAGCC CTACCGGGGC AGAGGGGGAA GCTGGGCAGA GCCAAGAGGA GAAATCCAGC 
CCAGCATCCC AAGCTGGCTC TCATGCTACG GCCCCCACCT CCGGCAGTAC GGTTCTAGAA 
AGAGGAACTG CAGGGTCCTT GTCCCCTGGC GTTGTGGAAG CTAATGCCCC CATGGTCCAA 
CCTAGTACAT CACCGGGCCC CGCACAGGAA GAGGCAGGAG AGAACCGCGT GGATGGTGAG 
AGAAATGTGA AGAGCTGCGC GTCTCCACGG GGCGGAGAAG CTGGGTTGCC GGAACCCAAC 
ACAGCTGGTC CGTCTTCAGA CACGGCAGAC GTTTCTAAAG AAGGAGGGAA CGATGAGGTG 
CTGACCCCTA CCAGACCCAG GACAACGGAA GACCTGTTTG CAGCCATTCA CAGATCCAAA 
AGGAAAGTCC TTGGTCGGAA GGACTCCGAA GATGATCACA CTCGAAATCA TTCTCCCTCC 
CCGCCTGTGA CACCCACAGG TGCTGCCCCT AGCCTGGCCT CTCCAAAGCA AGTAGGGTCC 
ATCCAGCGAA GCCTAAAAAA GAGCAGTACC AGCAGCGACA ACTTCAAAGC TCTGCTGCTC 
AAGAAGGGGA GTCGCTCGGA CACCAGCGCT CGCATGTCCG CGGCGGAGAT GCTCAAGTCC 
ACGGACCCTC GATTCCAGAG ATCTAGGTCG GAGCCTTCGC CAGAGGTCCC GGACAGCCCA 
TCCAGCTTCT CTCCCAATAA GAACAGGAGA GCTCCGGAGG AGTGGGCCAA GAATGAAGGA 
CTGATGCCTC GGAGTCTGTC CTTTTCCGGC CCTCGGTACA GCCGCAGCAG GACGCCACCC 
TCTGCGGCCA GCAGCAGGTA CAGCATGAGG AACCGCATCC AAAGCAGCCC CATGACAGTC 
ATCTCTGAGG GAGAGGGAGA GCCTGCCGAG CCCGCAGACA ACAAAGTACG CAGGACCCTG 
GACACTACGA GGGGATGCTC TCTGGACAGA CTGGCGGGAC AGGAGACGGA CCGGGGCAGC 
CCGCTGTGTA GCGAGGAGCC TGCCTCTGTG GATGGGATAG GAAGGGCTAA GGACGACGGT 
CCCTCGGAGC AGTGTAGGGG TCCTGAACAG AAGAGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006227733.1
CDS	1534..6252
Translation	MNTVSNLDEESRWTVHYTAPWHQQENVFLPSTRPPCVEDLHRQAKLNLKSVLRECDKLRRDGCRSSQYYSQGPTFAASSSPGDDYQEEDAETDRKYSLSSSEEERFIGIRRPKTPTSGDFSDLHTQTNWTKSLPLPTPEEKTRQQAQTVQADVVPINITGENFDRQASLRRSLIYTDTLVRRPKKVKRRKTISGIPDIIQKELASATGQEDDGSTHSLYSPDHYSTLGRLDSYRSTGQCLETRDSGCQTEEVKVIPPSVRRIRAHKGLGIAAQMSHLSGSSGNMSVLSDSAGIVFPSRLNNDTGFHSLPRAGARASTYSLEARMGALGSAEDTDDTFPYRGGSSQGPENFAHLGGASSTGMLSRPKSQQLRLLESPACVVSPHAAYSTSVIPNATLLSSSEVIVIHTAQSAGQLDSRVPGSSSYSKIKPRDHPTPRCSARDERQSPRHHWNEGHIIHSQALASAAPGATALLSLRDSEVSLNAPAINRENGSQAILYHCRSNLAFPAHPPDVDGKSECSSSGDRGCGSSESWEYKASSNGRASPLKPHLATPGCSTPTSNMSSCSLDQASLKEDARSLYSEDHDGYYMTAQGDSRHEAGSAYGISDGFGNPRHSTVNVFDGRAQRSQGDQATQQDRILSRNISLKKAKKPPPPPSRTDSLRRIPKKNNQTNGQVLNESLIASLQHSLQLSLPGQGSGSPSQSPCSDCEEPWLPRSRSQSIVSEGSSLTSTTTPNVYSLCGLTPSQSDTSSVKSEYADPWGYYIDYTTLQGDPGNPTGGCPASSEAATGNGPVRHIQEGSRAPVPQVPGCSVKPKITSPEKSQRVTSPSSGYSSQSNTPTALTPVPVFLKSMSPANGKGKAKPKVPERKSSLISSVSISSSSTSLSSNTSTEGSGTVKKLDSALASALAPPPPPLPALPSPSLANKSPFLPPPPPLADCSEGSPLPPSPVFPPPPPEALVPFCSPTDRCLSPSPTAFSPPLPRCSPPLPAPPPFLPPSEPPPAPPLDPKFMKENRPFIKNSSQSESSREALRRPANKEEGCRPSMPLITTEALQMVQLRPVRKNSGAETVLFSEPSAQEPRTPTAPQYHLKPSAFLKSRNSINEMESESQAASVTSSLPMPAKSQSQGDHDSAVERGGLQSCSDGAPGPGPSLRTALLPDSSPSRKPPPISKKPKLFLVVPPPQRDFTAEPTENGSEAFSGVPSPTGAEGEAGQSQEEKSSPASQAGSHATAPTSGSTVLERGTAGSLSPGVVEANAPMVQPSTSPGPAQEEAGENRVDGERNVKSCASPRGGEAGLPEPNTAGPSSDTADVSKEGGNDEVLTPTRPRTTEDLFAAIHRSKRKVLGRKDSEDDHTRNHSPSPPVTPTGAAPSLASPKQVGSIQRSLKKSSTSSDNFKALLLKKGSRSDTSARMSAAEMLKSTDPRFQRSRSEPSPEVPDSPSSFSPNKNRRAPEEWAKNEGLMPRSLSFSGPRYSRSRTPPSAASSRYSMRNRIQSSPMTVISEGEGEPAEPADNKVRRTLDTTRGCSLDRLAGQETDRGSPLCSEEPASVDGIGRAKDDGPSEQCRGPEQKS

Target ORF information:

RefSeq Version	XM_006227671.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus NHS-like 1 (Nhsl1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006227671.3

ORF Insert Sequence:

ATGAATACGG TTTCCAACTT AGACGAGGAG AGCCGATGGA CCGTCCACTA TACGGCGCCG 
TGGCACCAGC AGGAGAATGT GTTCCTCCCC TCCACAAGAC CCCCCTGTGT TGAGGACCTG 
CACCGCCAAG CGAAGCTCAA CCTCAAATCA GTGCTGAGGG AATGTGACAA GCTGAGGCGC 
GATGGCTGCC GAAGTTCTCA GTACTATTCT CAGGGACCCA CCTTTGCAGC CAGCTCCAGC 
CCAGGCGATG ACTATCAAGA GGAAGATGCA GAAACAGACC GAAAGTATTC TCTCTCTTCA 
TCTGAAGAAG AAAGATTTAT TGGTATCAGG AGACCTAAAA CGCCAACCTC AGGTGACTTC 
TCTGACCTTC ATACTCAGAC AAACTGGACC AAGTCCCTGC CGCTGCCAAC ACCAGAAGAG 
AAGACACGAC AGCAAGCCCA GACAGTCCAG GCTGATGTGG TTCCTATTAA CATAACTGGG 
GAGAATTTTG ATCGTCAGGC CAGCCTTCGG CGGTCTCTAA TTTACACTGA CACTCTGGTA 
AGACGACCGA AGAAAGTCAA AAGGAGAAAG ACTATTTCAG GAATCCCTGA CATCATTCAG 
AAGGAGCTAG CGTCAGCCAC GGGCCAAGAG GATGATGGTA GCACCCATTC TCTATACTCC 
CCAGACCACT ACTCTACATT AGGAAGGCTT GATAGCTATC GGTCCACGGG GCAATGCTTG 
GAAACCAGGG ACTCTGGCTG TCAGACAGAA GAAGTGAAAG TCATCCCACC GTCAGTGAGA 
AGGATCAGGG CTCACAAGGG GCTGGGCATT GCTGCCCAGA TGAGCCACCT CTCGGGTTCC 
TCTGGCAACA TGTCTGTGCT GAGCGACTCG GCTGGTATCG TGTTCCCATC TCGCCTCAAT 
AACGATACTG GTTTCCACAG CCTTCCACGC GCTGGCGCAA GGGCAAGCAC GTATTCCCTG 
GAGGCAAGGA TGGGTGCCCT GGGCTCTGCT GAAGATACGG ATGATACTTT TCCCTACCGA 
GGGGGTAGCT CCCAGGGGCC TGAAAACTTT GCACATTTAG GGGGTGCCTC GAGTACCGGG 
ATGCTTTCGA GGCCCAAATC CCAGCAGCTG AGGCTCTTGG AGAGCCCAGC GTGTGTGGTT 
TCACCGCACG CGGCCTACTC TACCAGCGTC ATCCCAAACG CCACACTGCT GTCCTCTTCG 
GAGGTTATTG TCATCCACAC CGCTCAGAGT GCAGGACAGC TGGACAGTAG AGTCCCCGGC 
TCATCCTCAT ACTCAAAGAT AAAACCCAGA GACCATCCCA CACCCAGGTG CTCTGCGAGA 
GATGAGCGCC AGTCCCCACG ACATCACTGG AACGAGGGTC ATATCATTCA CTCACAGGCT 
TTAGCCTCCG CTGCCCCCGG TGCCACCGCA CTGTTATCCC TCCGTGACTC AGAGGTCTCT 
CTCAATGCCC CAGCTATCAA TAGGGAGAAC GGGTCCCAAG CCATACTTTA TCACTGTAGA 
AGCAACCTGG CCTTTCCTGC CCACCCTCCG GATGTGGATG GCAAGAGCGA GTGCAGTTCT 
TCAGGGGACA GGGGATGTGG CAGTTCTGAG TCCTGGGAAT ACAAAGCCTC CAGCAATGGG 
CGGGCTTCCC CACTGAAGCC GCACCTTGCG ACTCCCGGTT GCTCTACCCC CACAAGTAAC 
ATGAGCAGCT GCAGCTTGGA CCAAGCATCC CTCAAGGAGG ATGCCAGGTC CCTGTATTCA 
GAGGACCACG ATGGTTACTA TATGACCGCC CAGGGTGATT CTAGACACGA GGCTGGGAGC 
GCGTACGGTA TCAGTGATGG CTTTGGAAAC CCCAGGCACA GCACGGTCAA TGTTTTTGAC 
GGAAGGGCTC AGAGAAGCCA AGGGGATCAG GCCACTCAGC AGGATAGAAT CCTCTCAAGA 
AACATCTCTC TGAAGAAAGC AAAAAAACCA CCACCGCCAC CATCCCGGAC AGATTCTCTG 
CGAAGGATTC CCAAGAAGAA CAACCAGACA AATGGGCAGG TGCTCAACGA GAGCCTGATC 
GCCTCACTCC AGCATTCTCT GCAGCTAAGC CTCCCTGGCC AAGGCAGCGG TTCCCCTTCC 
CAGAGCCCCT GCAGTGACTG TGAGGAACCC TGGCTCCCCA GGTCCAGAAG CCAGAGCATT 
GTTAGCGAGG GTAGTAGCTT GACCTCCACC ACCACTCCCA ACGTGTATTC TCTGTGCGGG 
CTCACTCCAT CTCAGAGCGA CACAAGCAGC GTCAAGTCGG AATACGCAGA CCCCTGGGGC 
TACTACATTG ACTACACAAC TTTGCAGGGA GATCCAGGGA ACCCCACAGG GGGCTGCCCA 
GCCAGTAGTG AGGCAGCCAC TGGAAATGGG CCAGTGCGCC ACATCCAGGA GGGGTCGAGA 
GCCCCAGTGC CCCAAGTGCC GGGCTGCTCC GTGAAACCAA AGATCACCTC ACCGGAGAAG 
TCACAGAGAG TCACCTCTCC ATCCAGTGGG TATTCTAGCC AGTCGAATAC ACCCACAGCA 
CTCACCCCCG TGCCTGTGTT TTTAAAATCG ATGTCACCAG CAAATGGGAA GGGGAAGGCC 
AAGCCCAAGG TACCAGAAAG AAAATCGTCT CTGATCTCTT CAGTGTCCAT CTCCTCATCG 
TCCACTTCCC TCTCCTCTAA TACCTCTACG GAAGGAAGTG GGACCGTGAA GAAACTGGAT 
TCAGCCCTGG CCTCGGCCCT TGCTCCTCCC CCTCCTCCTC TTCCCGCGCT GCCTTCTCCG 
AGTCTGGCAA ACAAGTCCCC CTTCCTTCCA CCTCCTCCTC CTCTAGCAGA TTGCTCCGAG 
GGCTCTCCCT TACCGCCCTC TCCTGTGTTC CCCCCTCCAC CACCAGAAGC CCTTGTTCCC 
TTCTGCTCCC CCACTGACAG GTGCCTTTCT CCTTCCCCCA CAGCTTTTAG CCCCCCTCTT 
CCAAGATGCT CACCTCCCTT GCCAGCACCT CCACCTTTCT TGCCCCCATC AGAACCCCCA 
CCTGCTCCGC CTCTGGACCC CAAATTCATG AAAGAAAACA GGCCCTTTAT CAAAAACTCT 
AGCCAGTCCG AATCCTCTAG GGAGGCCCTC AGGCGGCCTG CCAACAAGGA GGAGGGCTGC 
CGACCCTCGA TGCCCCTGAT CACCACAGAA GCTTTGCAGA TGGTGCAGTT GAGGCCAGTG 
AGGAAGAACT CAGGTGCTGA GACGGTCCTG TTTTCTGAAC CATCAGCTCA GGAACCACGA 
ACCCCGACTG CTCCACAGTA TCACTTAAAG CCATCTGCTT TCCTCAAATC CCGAAATAGC 
ATAAATGAAA TGGAGAGTGA AAGCCAGGCT GCCTCTGTGA CGAGCTCGCT TCCGATGCCT 
GCCAAGAGCC AGAGTCAGGG TGACCATGAC AGTGCAGTCG AGCGTGGCGG CCTTCAGAGC 
TGCAGCGATG GAGCTCCAGG CCCGGGTCCC AGCCTCAGGA CCGCTCTGCT CCCAGACTCT 
TCACCCAGCA GGAAGCCACC CCCCATCTCC AAGAAGCCCA AACTGTTCCT GGTGGTACCA 
CCTCCGCAGA GAGATTTCAC AGCGGAGCCC ACAGAGAACG GGAGCGAAGC TTTCTCAGGC 
GTGCCCAGCC CTACCGGGGC AGAGGGGGAA GCTGGGCAGA GCCAAGAGGA GAAATCCAGC 
CCAGCATCCC AAGCTGGCTC TCATGCTACG GCCCCCACCT CCGGCAGTAC GGTTCTAGAA 
AGAGGAACTG CAGGGTCCTT GTCCCCTGGC GTTGTGGAAG CTAATGCCCC CATGGTCCAA 
CCTAGTACAT CACCGGGCCC CGCACAGGAA GAGGCAGGAG AGAACCGCGT GGATGGTGAG 
AGAAATGTGA AGAGCTGCGC GTCTCCACGG GGCGGAGAAG CTGGGTTGCC GGAACCCAAC 
ACAGCTGGTC CGTCTTCAGA CACGGCAGAC GTTTCTAAAG AAGGAGGGAA CGATGAGGTG 
CTGACCCCTA CCAGACCCAG GACAACGGAA GACCTGTTTG CAGCCATTCA CAGATCCAAA 
AGGAAAGTCC TTGGTCGGAA GGACTCCGAA GATGATCACA CTCGAAATCA TTCTCCCTCC 
CCGCCTGTGA CACCCACAGG TGCTGCCCCT AGCCTGGCCT CTCCAAAGCA AGTAGGGTCC 
ATCCAGCGAA GCCTAAAAAA GAGCAGTACC AGCAGCGACA ACTTCAAAGC TCTGCTGCTC 
AAGAAGGGGA GTCGCTCGGA CACCAGCGCT CGCATGTCCG CGGCGGAGAT GCTCAAGTCC 
ACGGACCCTC GATTCCAGAG ATCTAGGTCG GAGCCTTCGC CAGAGGTCCC GGACAGCCCA 
TCCAGCTTCT CTCCCAATAA GAACAGGAGA GCTCCGGAGG AGTGGGCCAA GAATGAAGGA 
CTGATGCCTC GGAGTCTGTC CTTTTCCGGC CCTCGGTACA GCCGCAGCAG GACGCCACCC 
TCTGCGGCCA GCAGCAGGTA CAGCATGAGG AACCGCATCC AAAGCAGCCC CATGACAGTC 
ATCTCTGAGG GAGAGGGAGA GCCTGCCGAG CCCGCAGACA ACAAAGTACG CAGGACCCTG 
GACACTACGA GGGGATGCTC TCTGGACAGA CTGGCGGGAC AGGAGACGGA CCGGGGCAGC 
CCGCTGTGTA GCGAGGAGCC TGCCTCTGTG GATGGGATAG GAAGGGCTAA GGACGACGGT 
CCCTCGGAGC AGTGTAGGGG TCCTGAACAG AAGAGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nhsl1 ( XM_006222834.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006222896.1 Rattus norvegicus NHS-like 1 (Nhsl1), transcript variant X7, mRNA.

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CloneID	ORa13980
Clone ID Related Accession (Same CDS sequence)	XM_006227671.3 , XM_006222834.3
Accession Version	XM_006222834.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4719bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	NHS-like protein 1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (AC_000069.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006222834.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATACGG TTTCCAACTT AGACGAGGAG AGCCGATGGA CCGTCCACTA TACGGCGCCG 
TGGCACCAGC AGGAGAATGT GTTCCTCCCC TCCACAAGAC CCCCCTGTGT TGAGGACCTG 
CACCGCCAAG CGAAGCTCAA CCTCAAATCA GTGCTGAGGG AATGTGACAA GCTGAGGCGC 
GATGGCTGCC GAAGTTCTCA GTACTATTCT CAGGGACCCA CCTTTGCAGC CAGCTCCAGC 
CCAGGCGATG ACTATCAAGA GGAAGATGCA GAAACAGACC GAAAGTATTC TCTCTCTTCA 
TCTGAAGAAG AAAGATTTAT TGGTATCAGG AGACCTAAAA CGCCAACCTC AGGTGACTTC 
TCTGACCTTC ATACTCAGAC AAACTGGACC AAGTCCCTGC CGCTGCCAAC ACCAGAAGAG 
AAGACACGAC AGCAAGCCCA GACAGTCCAG GCTGATGTGG TTCCTATTAA CATAACTGGG 
GAGAATTTTG ATCGTCAGGC CAGCCTTCGG CGGTCTCTAA TTTACACTGA CACTCTGGTA 
AGACGACCGA AGAAAGTCAA AAGGAGAAAG ACTATTTCAG GAATCCCTGA CATCATTCAG 
AAGGAGCTAG CGTCAGCCAC GGGCCAAGAG GATGATGGTA GCACCCATTC TCTATACTCC 
CCAGACCACT ACTCTACATT AGGAAGGCTT GATAGCTATC GGTCCACGGG GCAATGCTTG 
GAAACCAGGG ACTCTGGCTG TCAGACAGAA GAAGTGAAAG TCATCCCACC GTCAGTGAGA 
AGGATCAGGG CTCACAAGGG GCTGGGCATT GCTGCCCAGA TGAGCCACCT CTCGGGTTCC 
TCTGGCAACA TGTCTGTGCT GAGCGACTCG GCTGGTATCG TGTTCCCATC TCGCCTCAAT 
AACGATACTG GTTTCCACAG CCTTCCACGC GCTGGCGCAA GGGCAAGCAC GTATTCCCTG 
GAGGCAAGGA TGGGTGCCCT GGGCTCTGCT GAAGATACGG ATGATACTTT TCCCTACCGA 
GGGGGTAGCT CCCAGGGGCC TGAAAACTTT GCACATTTAG GGGGTGCCTC GAGTACCGGG 
ATGCTTTCGA GGCCCAAATC CCAGCAGCTG AGGCTCTTGG AGAGCCCAGC GTGTGTGGTT 
TCACCGCACG CGGCCTACTC TACCAGCGTC ATCCCAAACG CCACACTGCT GTCCTCTTCG 
GAGGTTATTG TCATCCACAC CGCTCAGAGT GCAGGACAGC TGGACAGTAG AGTCCCCGGC 
TCATCCTCAT ACTCAAAGAT AAAACCCAGA GACCATCCCA CACCCAGGTG CTCTGCGAGA 
GATGAGCGCC AGTCCCCACG ACATCACTGG AACGAGGGTC ATATCATTCA CTCACAGGCT 
TTAGCCTCCG CTGCCCCCGG TGCCACCGCA CTGTTATCCC TCCGTGACTC AGAGGTCTCT 
CTCAATGCCC CAGCTATCAA TAGGGAGAAC GGGTCCCAAG CCATACTTTA TCACTGTAGA 
AGCAACCTGG CCTTTCCTGC CCACCCTCCG GATGTGGATG GCAAGAGCGA GTGCAGTTCT 
TCAGGGGACA GGGGATGTGG CAGTTCTGAG TCCTGGGAAT ACAAAGCCTC CAGCAATGGG 
CGGGCTTCCC CACTGAAGCC GCACCTTGCG ACTCCCGGTT GCTCTACCCC CACAAGTAAC 
ATGAGCAGCT GCAGCTTGGA CCAAGCATCC CTCAAGGAGG ATGCCAGGTC CCTGTATTCA 
GAGGACCACG ATGGTTACTA TATGACCGCC CAGGGTGATT CTAGACACGA GGCTGGGAGC 
GCGTACGGTA TCAGTGATGG CTTTGGAAAC CCCAGGCACA GCACGGTCAA TGTTTTTGAC 
GGAAGGGCTC AGAGAAGCCA AGGGGATCAG GCCACTCAGC AGGATAGAAT CCTCTCAAGA 
AACATCTCTC TGAAGAAAGC AAAAAAACCA CCACCGCCAC CATCCCGGAC AGATTCTCTG 
CGAAGGATTC CCAAGAAGAA CAACCAGACA AATGGGCAGG TGCTCAACGA GAGCCTGATC 
GCCTCACTCC AGCATTCTCT GCAGCTAAGC CTCCCTGGCC AAGGCAGCGG TTCCCCTTCC 
CAGAGCCCCT GCAGTGACTG TGAGGAACCC TGGCTCCCCA GGTCCAGAAG CCAGAGCATT 
GTTAGCGAGG GTAGTAGCTT GACCTCCACC ACCACTCCCA ACGTGTATTC TCTGTGCGGG 
CTCACTCCAT CTCAGAGCGA CACAAGCAGC GTCAAGTCGG AATACGCAGA CCCCTGGGGC 
TACTACATTG ACTACACAAC TTTGCAGGGA GATCCAGGGA ACCCCACAGG GGGCTGCCCA 
GCCAGTAGTG AGGCAGCCAC TGGAAATGGG CCAGTGCGCC ACATCCAGGA GGGGTCGAGA 
GCCCCAGTGC CCCAAGTGCC GGGCTGCTCC GTGAAACCAA AGATCACCTC ACCGGAGAAG 
TCACAGAGAG TCACCTCTCC ATCCAGTGGG TATTCTAGCC AGTCGAATAC ACCCACAGCA 
CTCACCCCCG TGCCTGTGTT TTTAAAATCG ATGTCACCAG CAAATGGGAA GGGGAAGGCC 
AAGCCCAAGG TACCAGAAAG AAAATCGTCT CTGATCTCTT CAGTGTCCAT CTCCTCATCG 
TCCACTTCCC TCTCCTCTAA TACCTCTACG GAAGGAAGTG GGACCGTGAA GAAACTGGAT 
TCAGCCCTGG CCTCGGCCCT TGCTCCTCCC CCTCCTCCTC TTCCCGCGCT GCCTTCTCCG 
AGTCTGGCAA ACAAGTCCCC CTTCCTTCCA CCTCCTCCTC CTCTAGCAGA TTGCTCCGAG 
GGCTCTCCCT TACCGCCCTC TCCTGTGTTC CCCCCTCCAC CACCAGAAGC CCTTGTTCCC 
TTCTGCTCCC CCACTGACAG GTGCCTTTCT CCTTCCCCCA CAGCTTTTAG CCCCCCTCTT 
CCAAGATGCT CACCTCCCTT GCCAGCACCT CCACCTTTCT TGCCCCCATC AGAACCCCCA 
CCTGCTCCGC CTCTGGACCC CAAATTCATG AAAGAAAACA GGCCCTTTAT CAAAAACTCT 
AGCCAGTCCG AATCCTCTAG GGAGGCCCTC AGGCGGCCTG CCAACAAGGA GGAGGGCTGC 
CGACCCTCGA TGCCCCTGAT CACCACAGAA GCTTTGCAGA TGGTGCAGTT GAGGCCAGTG 
AGGAAGAACT CAGGTGCTGA GACGGTCCTG TTTTCTGAAC CATCAGCTCA GGAACCACGA 
ACCCCGACTG CTCCACAGTA TCACTTAAAG CCATCTGCTT TCCTCAAATC CCGAAATAGC 
ATAAATGAAA TGGAGAGTGA AAGCCAGGCT GCCTCTGTGA CGAGCTCGCT TCCGATGCCT 
GCCAAGAGCC AGAGTCAGGG TGACCATGAC AGTGCAGTCG AGCGTGGCGG CCTTCAGAGC 
TGCAGCGATG GAGCTCCAGG CCCGGGTCCC AGCCTCAGGA CCGCTCTGCT CCCAGACTCT 
TCACCCAGCA GGAAGCCACC CCCCATCTCC AAGAAGCCCA AACTGTTCCT GGTGGTACCA 
CCTCCGCAGA GAGATTTCAC AGCGGAGCCC ACAGAGAACG GGAGCGAAGC TTTCTCAGGC 
GTGCCCAGCC CTACCGGGGC AGAGGGGGAA GCTGGGCAGA GCCAAGAGGA GAAATCCAGC 
CCAGCATCCC AAGCTGGCTC TCATGCTACG GCCCCCACCT CCGGCAGTAC GGTTCTAGAA 
AGAGGAACTG CAGGGTCCTT GTCCCCTGGC GTTGTGGAAG CTAATGCCCC CATGGTCCAA 
CCTAGTACAT CACCGGGCCC CGCACAGGAA GAGGCAGGAG AGAACCGCGT GGATGGTGAG 
AGAAATGTGA AGAGCTGCGC GTCTCCACGG GGCGGAGAAG CTGGGTTGCC GGAACCCAAC 
ACAGCTGGTC CGTCTTCAGA CACGGCAGAC GTTTCTAAAG AAGGAGGGAA CGATGAGGTG 
CTGACCCCTA CCAGACCCAG GACAACGGAA GACCTGTTTG CAGCCATTCA CAGATCCAAA 
AGGAAAGTCC TTGGTCGGAA GGACTCCGAA GATGATCACA CTCGAAATCA TTCTCCCTCC 
CCGCCTGTGA CACCCACAGG TGCTGCCCCT AGCCTGGCCT CTCCAAAGCA AGTAGGGTCC 
ATCCAGCGAA GCCTAAAAAA GAGCAGTACC AGCAGCGACA ACTTCAAAGC TCTGCTGCTC 
AAGAAGGGGA GTCGCTCGGA CACCAGCGCT CGCATGTCCG CGGCGGAGAT GCTCAAGTCC 
ACGGACCCTC GATTCCAGAG ATCTAGGTCG GAGCCTTCGC CAGAGGTCCC GGACAGCCCA 
TCCAGCTTCT CTCCCAATAA GAACAGGAGA GCTCCGGAGG AGTGGGCCAA GAATGAAGGA 
CTGATGCCTC GGAGTCTGTC CTTTTCCGGC CCTCGGTACA GCCGCAGCAG GACGCCACCC 
TCTGCGGCCA GCAGCAGGTA CAGCATGAGG AACCGCATCC AAAGCAGCCC CATGACAGTC 
ATCTCTGAGG GAGAGGGAGA GCCTGCCGAG CCCGCAGACA ACAAAGTACG CAGGACCCTG 
GACACTACGA GGGGATGCTC TCTGGACAGA CTGGCGGGAC AGGAGACGGA CCGGGGCAGC 
CCGCTGTGTA GCGAGGAGCC TGCCTCTGTG GATGGGATAG GAAGGGCTAA GGACGACGGT 
CCCTCGGAGC AGTGTAGGGG TCCTGAACAG AAGAGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006222896.1
CDS	1534..6252
Translation	MNTVSNLDEESRWTVHYTAPWHQQENVFLPSTRPPCVEDLHRQAKLNLKSVLRECDKLRRDGCRSSQYYSQGPTFAASSSPGDDYQEEDAETDRKYSLSSSEEERFIGIRRPKTPTSGDFSDLHTQTNWTKSLPLPTPEEKTRQQAQTVQADVVPINITGENFDRQASLRRSLIYTDTLVRRPKKVKRRKTISGIPDIIQKELASATGQEDDGSTHSLYSPDHYSTLGRLDSYRSTGQCLETRDSGCQTEEVKVIPPSVRRIRAHKGLGIAAQMSHLSGSSGNMSVLSDSAGIVFPSRLNNDTGFHSLPRAGARASTYSLEARMGALGSAEDTDDTFPYRGGSSQGPENFAHLGGASSTGMLSRPKSQQLRLLESPACVVSPHAAYSTSVIPNATLLSSSEVIVIHTAQSAGQLDSRVPGSSSYSKIKPRDHPTPRCSARDERQSPRHHWNEGHIIHSQALASAAPGATALLSLRDSEVSLNAPAINRENGSQAILYHCRSNLAFPAHPPDVDGKSECSSSGDRGCGSSESWEYKASSNGRASPLKPHLATPGCSTPTSNMSSCSLDQASLKEDARSLYSEDHDGYYMTAQGDSRHEAGSAYGISDGFGNPRHSTVNVFDGRAQRSQGDQATQQDRILSRNISLKKAKKPPPPPSRTDSLRRIPKKNNQTNGQVLNESLIASLQHSLQLSLPGQGSGSPSQSPCSDCEEPWLPRSRSQSIVSEGSSLTSTTTPNVYSLCGLTPSQSDTSSVKSEYADPWGYYIDYTTLQGDPGNPTGGCPASSEAATGNGPVRHIQEGSRAPVPQVPGCSVKPKITSPEKSQRVTSPSSGYSSQSNTPTALTPVPVFLKSMSPANGKGKAKPKVPERKSSLISSVSISSSSTSLSSNTSTEGSGTVKKLDSALASALAPPPPPLPALPSPSLANKSPFLPPPPPLADCSEGSPLPPSPVFPPPPPEALVPFCSPTDRCLSPSPTAFSPPLPRCSPPLPAPPPFLPPSEPPPAPPLDPKFMKENRPFIKNSSQSESSREALRRPANKEEGCRPSMPLITTEALQMVQLRPVRKNSGAETVLFSEPSAQEPRTPTAPQYHLKPSAFLKSRNSINEMESESQAASVTSSLPMPAKSQSQGDHDSAVERGGLQSCSDGAPGPGPSLRTALLPDSSPSRKPPPISKKPKLFLVVPPPQRDFTAEPTENGSEAFSGVPSPTGAEGEAGQSQEEKSSPASQAGSHATAPTSGSTVLERGTAGSLSPGVVEANAPMVQPSTSPGPAQEEAGENRVDGERNVKSCASPRGGEAGLPEPNTAGPSSDTADVSKEGGNDEVLTPTRPRTTEDLFAAIHRSKRKVLGRKDSEDDHTRNHSPSPPVTPTGAAPSLASPKQVGSIQRSLKKSSTSSDNFKALLLKKGSRSDTSARMSAAEMLKSTDPRFQRSRSEPSPEVPDSPSSFSPNKNRRAPEEWAKNEGLMPRSLSFSGPRYSRSRTPPSAASSRYSMRNRIQSSPMTVISEGEGEPAEPADNKVRRTLDTTRGCSLDRLAGQETDRGSPLCSEEPASVDGIGRAKDDGPSEQCRGPEQKS

Target ORF information:

RefSeq Version	XM_006222834.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus NHS-like 1 (Nhsl1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006222834.3

ORF Insert Sequence:

ATGAATACGG TTTCCAACTT AGACGAGGAG AGCCGATGGA CCGTCCACTA TACGGCGCCG 
TGGCACCAGC AGGAGAATGT GTTCCTCCCC TCCACAAGAC CCCCCTGTGT TGAGGACCTG 
CACCGCCAAG CGAAGCTCAA CCTCAAATCA GTGCTGAGGG AATGTGACAA GCTGAGGCGC 
GATGGCTGCC GAAGTTCTCA GTACTATTCT CAGGGACCCA CCTTTGCAGC CAGCTCCAGC 
CCAGGCGATG ACTATCAAGA GGAAGATGCA GAAACAGACC GAAAGTATTC TCTCTCTTCA 
TCTGAAGAAG AAAGATTTAT TGGTATCAGG AGACCTAAAA CGCCAACCTC AGGTGACTTC 
TCTGACCTTC ATACTCAGAC AAACTGGACC AAGTCCCTGC CGCTGCCAAC ACCAGAAGAG 
AAGACACGAC AGCAAGCCCA GACAGTCCAG GCTGATGTGG TTCCTATTAA CATAACTGGG 
GAGAATTTTG ATCGTCAGGC CAGCCTTCGG CGGTCTCTAA TTTACACTGA CACTCTGGTA 
AGACGACCGA AGAAAGTCAA AAGGAGAAAG ACTATTTCAG GAATCCCTGA CATCATTCAG 
AAGGAGCTAG CGTCAGCCAC GGGCCAAGAG GATGATGGTA GCACCCATTC TCTATACTCC 
CCAGACCACT ACTCTACATT AGGAAGGCTT GATAGCTATC GGTCCACGGG GCAATGCTTG 
GAAACCAGGG ACTCTGGCTG TCAGACAGAA GAAGTGAAAG TCATCCCACC GTCAGTGAGA 
AGGATCAGGG CTCACAAGGG GCTGGGCATT GCTGCCCAGA TGAGCCACCT CTCGGGTTCC 
TCTGGCAACA TGTCTGTGCT GAGCGACTCG GCTGGTATCG TGTTCCCATC TCGCCTCAAT 
AACGATACTG GTTTCCACAG CCTTCCACGC GCTGGCGCAA GGGCAAGCAC GTATTCCCTG 
GAGGCAAGGA TGGGTGCCCT GGGCTCTGCT GAAGATACGG ATGATACTTT TCCCTACCGA 
GGGGGTAGCT CCCAGGGGCC TGAAAACTTT GCACATTTAG GGGGTGCCTC GAGTACCGGG 
ATGCTTTCGA GGCCCAAATC CCAGCAGCTG AGGCTCTTGG AGAGCCCAGC GTGTGTGGTT 
TCACCGCACG CGGCCTACTC TACCAGCGTC ATCCCAAACG CCACACTGCT GTCCTCTTCG 
GAGGTTATTG TCATCCACAC CGCTCAGAGT GCAGGACAGC TGGACAGTAG AGTCCCCGGC 
TCATCCTCAT ACTCAAAGAT AAAACCCAGA GACCATCCCA CACCCAGGTG CTCTGCGAGA 
GATGAGCGCC AGTCCCCACG ACATCACTGG AACGAGGGTC ATATCATTCA CTCACAGGCT 
TTAGCCTCCG CTGCCCCCGG TGCCACCGCA CTGTTATCCC TCCGTGACTC AGAGGTCTCT 
CTCAATGCCC CAGCTATCAA TAGGGAGAAC GGGTCCCAAG CCATACTTTA TCACTGTAGA 
AGCAACCTGG CCTTTCCTGC CCACCCTCCG GATGTGGATG GCAAGAGCGA GTGCAGTTCT 
TCAGGGGACA GGGGATGTGG CAGTTCTGAG TCCTGGGAAT ACAAAGCCTC CAGCAATGGG 
CGGGCTTCCC CACTGAAGCC GCACCTTGCG ACTCCCGGTT GCTCTACCCC CACAAGTAAC 
ATGAGCAGCT GCAGCTTGGA CCAAGCATCC CTCAAGGAGG ATGCCAGGTC CCTGTATTCA 
GAGGACCACG ATGGTTACTA TATGACCGCC CAGGGTGATT CTAGACACGA GGCTGGGAGC 
GCGTACGGTA TCAGTGATGG CTTTGGAAAC CCCAGGCACA GCACGGTCAA TGTTTTTGAC 
GGAAGGGCTC AGAGAAGCCA AGGGGATCAG GCCACTCAGC AGGATAGAAT CCTCTCAAGA 
AACATCTCTC TGAAGAAAGC AAAAAAACCA CCACCGCCAC CATCCCGGAC AGATTCTCTG 
CGAAGGATTC CCAAGAAGAA CAACCAGACA AATGGGCAGG TGCTCAACGA GAGCCTGATC 
GCCTCACTCC AGCATTCTCT GCAGCTAAGC CTCCCTGGCC AAGGCAGCGG TTCCCCTTCC 
CAGAGCCCCT GCAGTGACTG TGAGGAACCC TGGCTCCCCA GGTCCAGAAG CCAGAGCATT 
GTTAGCGAGG GTAGTAGCTT GACCTCCACC ACCACTCCCA ACGTGTATTC TCTGTGCGGG 
CTCACTCCAT CTCAGAGCGA CACAAGCAGC GTCAAGTCGG AATACGCAGA CCCCTGGGGC 
TACTACATTG ACTACACAAC TTTGCAGGGA GATCCAGGGA ACCCCACAGG GGGCTGCCCA 
GCCAGTAGTG AGGCAGCCAC TGGAAATGGG CCAGTGCGCC ACATCCAGGA GGGGTCGAGA 
GCCCCAGTGC CCCAAGTGCC GGGCTGCTCC GTGAAACCAA AGATCACCTC ACCGGAGAAG 
TCACAGAGAG TCACCTCTCC ATCCAGTGGG TATTCTAGCC AGTCGAATAC ACCCACAGCA 
CTCACCCCCG TGCCTGTGTT TTTAAAATCG ATGTCACCAG CAAATGGGAA GGGGAAGGCC 
AAGCCCAAGG TACCAGAAAG AAAATCGTCT CTGATCTCTT CAGTGTCCAT CTCCTCATCG 
TCCACTTCCC TCTCCTCTAA TACCTCTACG GAAGGAAGTG GGACCGTGAA GAAACTGGAT 
TCAGCCCTGG CCTCGGCCCT TGCTCCTCCC CCTCCTCCTC TTCCCGCGCT GCCTTCTCCG 
AGTCTGGCAA ACAAGTCCCC CTTCCTTCCA CCTCCTCCTC CTCTAGCAGA TTGCTCCGAG 
GGCTCTCCCT TACCGCCCTC TCCTGTGTTC CCCCCTCCAC CACCAGAAGC CCTTGTTCCC 
TTCTGCTCCC CCACTGACAG GTGCCTTTCT CCTTCCCCCA CAGCTTTTAG CCCCCCTCTT 
CCAAGATGCT CACCTCCCTT GCCAGCACCT CCACCTTTCT TGCCCCCATC AGAACCCCCA 
CCTGCTCCGC CTCTGGACCC CAAATTCATG AAAGAAAACA GGCCCTTTAT CAAAAACTCT 
AGCCAGTCCG AATCCTCTAG GGAGGCCCTC AGGCGGCCTG CCAACAAGGA GGAGGGCTGC 
CGACCCTCGA TGCCCCTGAT CACCACAGAA GCTTTGCAGA TGGTGCAGTT GAGGCCAGTG 
AGGAAGAACT CAGGTGCTGA GACGGTCCTG TTTTCTGAAC CATCAGCTCA GGAACCACGA 
ACCCCGACTG CTCCACAGTA TCACTTAAAG CCATCTGCTT TCCTCAAATC CCGAAATAGC 
ATAAATGAAA TGGAGAGTGA AAGCCAGGCT GCCTCTGTGA CGAGCTCGCT TCCGATGCCT 
GCCAAGAGCC AGAGTCAGGG TGACCATGAC AGTGCAGTCG AGCGTGGCGG CCTTCAGAGC 
TGCAGCGATG GAGCTCCAGG CCCGGGTCCC AGCCTCAGGA CCGCTCTGCT CCCAGACTCT 
TCACCCAGCA GGAAGCCACC CCCCATCTCC AAGAAGCCCA AACTGTTCCT GGTGGTACCA 
CCTCCGCAGA GAGATTTCAC AGCGGAGCCC ACAGAGAACG GGAGCGAAGC TTTCTCAGGC 
GTGCCCAGCC CTACCGGGGC AGAGGGGGAA GCTGGGCAGA GCCAAGAGGA GAAATCCAGC 
CCAGCATCCC AAGCTGGCTC TCATGCTACG GCCCCCACCT CCGGCAGTAC GGTTCTAGAA 
AGAGGAACTG CAGGGTCCTT GTCCCCTGGC GTTGTGGAAG CTAATGCCCC CATGGTCCAA 
CCTAGTACAT CACCGGGCCC CGCACAGGAA GAGGCAGGAG AGAACCGCGT GGATGGTGAG 
AGAAATGTGA AGAGCTGCGC GTCTCCACGG GGCGGAGAAG CTGGGTTGCC GGAACCCAAC 
ACAGCTGGTC CGTCTTCAGA CACGGCAGAC GTTTCTAAAG AAGGAGGGAA CGATGAGGTG 
CTGACCCCTA CCAGACCCAG GACAACGGAA GACCTGTTTG CAGCCATTCA CAGATCCAAA 
AGGAAAGTCC TTGGTCGGAA GGACTCCGAA GATGATCACA CTCGAAATCA TTCTCCCTCC 
CCGCCTGTGA CACCCACAGG TGCTGCCCCT AGCCTGGCCT CTCCAAAGCA AGTAGGGTCC 
ATCCAGCGAA GCCTAAAAAA GAGCAGTACC AGCAGCGACA ACTTCAAAGC TCTGCTGCTC 
AAGAAGGGGA GTCGCTCGGA CACCAGCGCT CGCATGTCCG CGGCGGAGAT GCTCAAGTCC 
ACGGACCCTC GATTCCAGAG ATCTAGGTCG GAGCCTTCGC CAGAGGTCCC GGACAGCCCA 
TCCAGCTTCT CTCCCAATAA GAACAGGAGA GCTCCGGAGG AGTGGGCCAA GAATGAAGGA 
CTGATGCCTC GGAGTCTGTC CTTTTCCGGC CCTCGGTACA GCCGCAGCAG GACGCCACCC 
TCTGCGGCCA GCAGCAGGTA CAGCATGAGG AACCGCATCC AAAGCAGCCC CATGACAGTC 
ATCTCTGAGG GAGAGGGAGA GCCTGCCGAG CCCGCAGACA ACAAAGTACG CAGGACCCTG 
GACACTACGA GGGGATGCTC TCTGGACAGA CTGGCGGGAC AGGAGACGGA CCGGGGCAGC 
CCGCTGTGTA GCGAGGAGCC TGCCTCTGTG GATGGGATAG GAAGGGCTAA GGACGACGGT 
CCCTCGGAGC AGTGTAGGGG TCCTGAACAG AAGAGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nhsl1 ( XM_017587917.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017443406.1 Rattus norvegicus NHS-like 1 (Nhsl1), transcript variant X1, mRNA.

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CloneID	ORa53614
Clone ID Related Accession (Same CDS sequence)	XM_017587917.1 , XM_017589823.1
Accession Version	XM_017587917.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5004bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	NHS-like protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (AC_000069.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCTTCC ACCAACGGAG CGTGGAGCCC GCACGGCTGC GCCGGCCCGA GGAGGCGGCG 
GGGGCTCTGG CTGGGGACCC GCCGCTCTTC CGCTCGCTGG AGCAGGTCAG CTCGCACACC 
CTGGTCTGCT TGCTCGCCCA GCTGGCCGAC CTGTCGCGCT GCGCTGGGGA CATTTTCGGG 
GAGCTCGAGG GCCAGGCGGC CGCGCTGGGT CACCGCACCG CCGAGTTGCA CCGTCGCCTG 
GACGCCCTGC ACGCTGCCGC CGCGCGCCTG GACCACCGGC GAGTGAAAAT CCCGGTTTCC 
AACTTAGACG AGGAGAGCCG ATGGACCGTC CACTATACGG CGCCGTGGCA CCAGCAGGAG 
AATGTGTTCC TCCCCTCCAC AAGACCCCCC TGTGTTGAGG ACCTGCACCG CCAAGCGAAG 
CTCAACCTCA AATCAGTGCT GAGGGAATGT GACAAGCTGA GGCGCGATGG CTGCCGAAGT 
TCTCAGTACT ATTCTCAGGG ACCCACCTTT GCAGCCAGCT CCAGCCCAGG CGATGACTAT 
CAAGAGGAAG ATGCAGAAAC AGACCGAAAG TATTCTCTCT CTTCATCTGA AGAAGAAAGA 
TTTATTGGTA TCAGGAGACC TAAAACGCCA ACCTCAGGTG ACTTCTCTGA CCTTCATACT 
CAGACAAACT GGACCAAGTC CCTGCCGCTG CCAACACCAG AAGAGAAGAC ACGACAGCAA 
GCCCAGACAG TCCAGGCTGA TGTGGTTCCT ATTAACATAA CTGGGGAGAA TTTTGATCGT 
CAGGCCAGCC TTCGGCGGTC TCTAATTTAC ACTGACACTC TGGTAAGACG ACCGAAGAAA 
GTCAAAAGGA GAAAGACTAT TTCAGGAATC CCTGACATCA TTCAGAAGGA GCTAGCGTCA 
GCCACGGGCC AAGAGGATGA TGGTAGCACC CATTCTCTAT ACTCCCCAGA CCACTACTCT 
ACATTAGGAA GGCTTGATAG CTATCGGTCC ACGGGGCAAT GCTTGGAAAC CAGGGACTCT 
GGCTGTCAGA CAGAAGAAGT GAAAGTCATC CCACCGTCAG TGAGAAGGAT CAGGGCTCAC 
AAGGGGCTGG GCATTGCTGC CCAGATGAGC CACCTCTCGG GTTCCTCTGG CAACATGTCT 
GTGCTGAGCG ACTCGGCTGG TATCGTGTTC CCATCTCGCC TCAATAACGA TACTGGTTTC 
CACAGCCTTC CACGCGCTGG CGCAAGGGCA AGCACGTATT CCCTGGAGGC AAGGATGGGT 
GCCCTGGGCT CTGCTGAAGA TACGGATGAT ACTTTTCCCT ACCGAGGGGG TAGCTCCCAG 
GGGCCTGAAA ACTTTGCACA TTTAGGGGGT GCCTCGAGTA CCGGGATGCT TTCGAGGCCC 
AAATCCCAGC AGCTGAGGCT CTTGGAGAGC CCAGCGTGTG TGGTTTCACC GCACGCGGCC 
TACTCTACCA GCGTCATCCC AAACGCCACA CTGCTGTCCT CTTCGGAGGT TATTGTCATC 
CACACCGCTC AGAGTGCAGG ACAGCTGGAC AGTAGAGTCC CCGGCTCATC CTCATACTCA 
AAGATAAAAC CCAGAGACCA TCCCACACCC AGGTGCTCTG CGAGAGATGA GCGCCAGTCC 
CCACGACATC ACTGGAACGA GGGTCATATC ATTCACTCAC AGGCTTTAGC CTCCGCTGCC 
CCCGGTGCCA CCGCACTGTT ATCCCTCCGT GACTCAGAGG TCTCTCTCAA TGCCCCAGCT 
ATCAATAGGG AGAACGGGTC CCAAGCCATA CTTTATCACT GTAGAAGCAA CCTGGCCTTT 
CCTGCCCACC CTCCGGATGT GGATGGCAAG AGCGAGTGCA GTTCTTCAGG GGACAGGGGA 
TGTGGCAGTT CTGAGTCCTG GGAATACAAA GCCTCCAGCA ATGGGCGGGC TTCCCCACTG 
AAGCCGCACC TTGCGACTCC CGGTTGCTCT ACCCCCACAA GTAACATGAG CAGCTGCAGC 
TTGGACCAAG CATCCCTCAA GGAGGATGCC AGGTCCCTGT ATTCAGAGGA CCACGATGGT 
TACTATATGA CCGCCCAGGG TGATTCTAGA CACGAGGCTG GGAGCGCGTA CGGTATCAGT 
GATGGCTTTG GAAACCCCAG GCACAGCACG GTCAATGTTT TTGACGGAAG GGCTCAGAGA 
AGCCAAGGGG ATCAGGCCAC TCAGCAGGAT AGAATCCTCT CAAGAAACAT CTCTCTGAAG 
AAAGCAAAAA AACCACCACC GCCACCATCC CGGACAGATT CTCTGCGAAG GATTCCCAAG 
AAGAACAACC AGACAAATGG GCAGGTGCTC AACGAGAGCC TGATCGCCTC ACTCCAGCAT 
TCTCTGCAGC TAAGCCTCCC TGGCCAAGGC AGCGGTTCCC CTTCCCAGAG CCCCTGCAGT 
GACTGTGAGG AACCCTGGCT CCCCAGGTCC AGAAGCCAGA GCATTGTTAG CGAGGGTAGT 
AGCTTGACCT CCACCACCAC TCCCAACGTG TATTCTCTGT GCGGGCTCAC TCCATCTCAG 
AGCGACACAA GCAGCGTCAA GTCGGAATAC GCAGACCCCT GGGGCTACTA CATTGACTAC 
ACAACTTTGC AGGGAGATCC AGGGAACCCC ACAGGGGGCT GCCCAGCCAG TAGTGAGGCA 
GCCACTGGAA ATGGGCCAGT GCGCCACATC CAGGAGGGGT CGAGAGCCCC AGTGCCCCAA 
GTGCCGGGCT GCTCCGTGAA ACCAAAGATC ACCTCACCGG AGAAGTCACA GAGAGTCACC 
TCTCCATCCA GTGGGTATTC TAGCCAGTCG AATACACCCA CAGCACTCAC CCCCGTGCCT 
GTGTTTTTAA AATCGATGTC ACCAGCAAAT GGGAAGGGGA AGGCCAAGCC CAAGGTACCA 
GAAAGAAAAT CGTCTCTGAT CTCTTCAGTG TCCATCTCCT CATCGTCCAC TTCCCTCTCC 
TCTAATACCT CTACGGAAGG AAGTGGGACC GTGAAGAAAC TGGATTCAGC CCTGGCCTCG 
GCCCTTGCTC CTCCCCCTCC TCCTCTTCCC GCGCTGCCTT CTCCGAGTCT GGCAAACAAG 
TCCCCCTTCC TTCCACCTCC TCCTCCTCTA GCAGATTGCT CCGAGGGCTC TCCCTTACCG 
CCCTCTCCTG TGTTCCCCCC TCCACCACCA GAAGCCCTTG TTCCCTTCTG CTCCCCCACT 
GACAGGTGCC TTTCTCCTTC CCCCACAGCT TTTAGCCCCC CTCTTCCAAG ATGCTCACCT 
CCCTTGCCAG CACCTCCACC TTTCTTGCCC CCATCAGAAC CCCCACCTGC TCCGCCTCTG 
GACCCCAAAT TCATGAAAGA AAACAGGCCC TTTATCAAAA ACTCTAGCCA GTCCGAATCC 
TCTAGGGAGG CCCTCAGGCG GCCTGCCAAC AAGGAGGAGG GCTGCCGACC CTCGATGCCC 
CTGATCACCA CAGAAGCTTT GCAGATGGTG CAGTTGAGGC CAGTGAGGAA GAACTCAGGT 
GCTGAGACGG TCCTGTTTTC TGAACCATCA GCTCAGGAAC CACGAACCCC GACTGCTCCA 
CAGTATCACT TAAAGCCATC TGCTTTCCTC AAATCCCGAA ATAGCATAAA TGAAATGGAG 
AGTGAAAGCC AGGCTGCCTC TGTGACGAGC TCGCTTCCGA TGCCTGCCAA GAGCCAGAGT 
CAGGGTGACC ATGACAGTGC AGTCGAGCGT GGCGGCCTTC AGAGCTGCAG CGATGGAGCT 
CCAGGCCCGG GTCCCAGCCT CAGGACCGCT CTGCTCCCAG ACTCTTCACC CAGCAGGAAG 
CCACCCCCCA TCTCCAAGAA GCCCAAACTG TTCCTGGTGG TACCACCTCC GCAGAGAGAT 
TTCACAGCGG AGCCCACAGA GAACGGGAGC GAAGCTTTCT CAGGCGTGCC CAGCCCTACC 
GGGGCAGAGG GGGAAGCTGG GCAGAGCCAA GAGGAGAAAT CCAGCCCAGC ATCCCAAGCT 
GGCTCTCATG CTACGGCCCC CACCTCCGGC AGTACGGTTC TAGAAAGAGG AACTGCAGGG 
TCCTTGTCCC CTGGCGTTGT GGAAGCTAAT GCCCCCATGG TCCAACCTAG TACATCACCG 
GGCCCCGCAC AGGAAGAGGC AGGAGAGAAC CGCGTGGATG GTGAGAGAAA TGTGAAGAGC 
TGCGCGTCTC CACGGGGCGG AGAAGCTGGG TTGCCGGAAC CCAACACAGC TGGTCCGTCT 
TCAGACACGG CAGACGTTTC TAAAGAAGGA GGGAACGATG AGGTGCTGAC CCCTACCAGA 
CCCAGGACAA CGGAAGACCT GTTTGCAGCC ATTCACAGAT CCAAAAGGAA AGTCCTTGGT 
CGGAAGGACT CCGAAGATGA TCACACTCGA AATCATTCTC CCTCCCCGCC TGTGACACCC 
ACAGGTGCTG CCCCTAGCCT GGCCTCTCCA AAGCAAGTAG GGTCCATCCA GCGAAGCCTA 
AAAAAGAGCA GTACCAGCAG CGACAACTTC AAAGCTCTGC TGCTCAAGAA GGGGAGTCGC 
TCGGACACCA GCGCTCGCAT GTCCGCGGCG GAGATGCTCA AGTCCACGGA CCCTCGATTC 
CAGAGATCTA GGTCGGAGCC TTCGCCAGAG GTCCCGGACA GCCCATCCAG CTTCTCTCCC 
AATAAGAACA GGAGAGCTCC GGAGGAGTGG GCCAAGAATG AAGGACTGAT GCCTCGGAGT 
CTGTCCTTTT CCGGCCCTCG GTACAGCCGC AGCAGGACGC CACCCTCTGC GGCCAGCAGC 
AGGTACAGCA TGAGGAACCG CATCCAAAGC AGCCCCATGA CAGTCATCTC TGAGGGAGAG 
GGAGAGCCTG CCGAGCCCGC AGACAACAAA GTACGCAGGA CCCTGGACAC TACGAGGGGA 
TGCTCTCTGG ACAGACTGGC GGGACAGGAG ACGGACCGGG GCAGCCCGCT GTGTAGCGAG 
GAGCCTGCCT CTGTGGATGG GATAGGAAGG GCTAAGGACG ACGGTCCCTC GGAGCAGTGT 
AGGGGTCCTG AACAGAAGAG TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017443406.1
CDS	288..5291
Translation	MPFHQRSVEPARLRRPEEAAGALAGDPPLFRSLEQVSSHTLVCLLAQLADLSRCAGDIFGELEGQAAALGHRTAELHRRLDALHAAAARLDHRRVKIPVSNLDEESRWTVHYTAPWHQQENVFLPSTRPPCVEDLHRQAKLNLKSVLRECDKLRRDGCRSSQYYSQGPTFAASSSPGDDYQEEDAETDRKYSLSSSEEERFIGIRRPKTPTSGDFSDLHTQTNWTKSLPLPTPEEKTRQQAQTVQADVVPINITGENFDRQASLRRSLIYTDTLVRRPKKVKRRKTISGIPDIIQKELASATGQEDDGSTHSLYSPDHYSTLGRLDSYRSTGQCLETRDSGCQTEEVKVIPPSVRRIRAHKGLGIAAQMSHLSGSSGNMSVLSDSAGIVFPSRLNNDTGFHSLPRAGARASTYSLEARMGALGSAEDTDDTFPYRGGSSQGPENFAHLGGASSTGMLSRPKSQQLRLLESPACVVSPHAAYSTSVIPNATLLSSSEVIVIHTAQSAGQLDSRVPGSSSYSKIKPRDHPTPRCSARDERQSPRHHWNEGHIIHSQALASAAPGATALLSLRDSEVSLNAPAINRENGSQAILYHCRSNLAFPAHPPDVDGKSECSSSGDRGCGSSESWEYKASSNGRASPLKPHLATPGCSTPTSNMSSCSLDQASLKEDARSLYSEDHDGYYMTAQGDSRHEAGSAYGISDGFGNPRHSTVNVFDGRAQRSQGDQATQQDRILSRNISLKKAKKPPPPPSRTDSLRRIPKKNNQTNGQVLNESLIASLQHSLQLSLPGQGSGSPSQSPCSDCEEPWLPRSRSQSIVSEGSSLTSTTTPNVYSLCGLTPSQSDTSSVKSEYADPWGYYIDYTTLQGDPGNPTGGCPASSEAATGNGPVRHIQEGSRAPVPQVPGCSVKPKITSPEKSQRVTSPSSGYSSQSNTPTALTPVPVFLKSMSPANGKGKAKPKVPERKSSLISSVSISSSSTSLSSNTSTEGSGTVKKLDSALASALAPPPPPLPALPSPSLANKSPFLPPPPPLADCSEGSPLPPSPVFPPPPPEALVPFCSPTDRCLSPSPTAFSPPLPRCSPPLPAPPPFLPPSEPPPAPPLDPKFMKENRPFIKNSSQSESSREALRRPANKEEGCRPSMPLITTEALQMVQLRPVRKNSGAETVLFSEPSAQEPRTPTAPQYHLKPSAFLKSRNSINEMESESQAASVTSSLPMPAKSQSQGDHDSAVERGGLQSCSDGAPGPGPSLRTALLPDSSPSRKPPPISKKPKLFLVVPPPQRDFTAEPTENGSEAFSGVPSPTGAEGEAGQSQEEKSSPASQAGSHATAPTSGSTVLERGTAGSLSPGVVEANAPMVQPSTSPGPAQEEAGENRVDGERNVKSCASPRGGEAGLPEPNTAGPSSDTADVSKEGGNDEVLTPTRPRTTEDLFAAIHRSKRKVLGRKDSEDDHTRNHSPSPPVTPTGAAPSLASPKQVGSIQRSLKKSSTSSDNFKALLLKKGSRSDTSARMSAAEMLKSTDPRFQRSRSEPSPEVPDSPSSFSPNKNRRAPEEWAKNEGLMPRSLSFSGPRYSRSRTPPSAASSRYSMRNRIQSSPMTVISEGEGEPAEPADNKVRRTLDTTRGCSLDRLAGQETDRGSPLCSEEPASVDGIGRAKDDGPSEQCRGPEQKS

Target ORF information:

RefSeq Version	XM_017587917.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus NHS-like 1 (Nhsl1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017587917.1

ORF Insert Sequence:

ATGCCCTTCC ACCAACGGAG CGTGGAGCCC GCACGGCTGC GCCGGCCCGA GGAGGCGGCG 
GGGGCTCTGG CTGGGGACCC GCCGCTCTTC CGCTCGCTGG AGCAGGTCAG CTCGCACACC 
CTGGTCTGCT TGCTCGCCCA GCTGGCCGAC CTGTCGCGCT GCGCTGGGGA CATTTTCGGG 
GAGCTCGAGG GCCAGGCGGC CGCGCTGGGT CACCGCACCG CCGAGTTGCA CCGTCGCCTG 
GACGCCCTGC ACGCTGCCGC CGCGCGCCTG GACCACCGGC GAGTGAAAAT CCCGGTTTCC 
AACTTAGACG AGGAGAGCCG ATGGACCGTC CACTATACGG CGCCGTGGCA CCAGCAGGAG 
AATGTGTTCC TCCCCTCCAC AAGACCCCCC TGTGTTGAGG ACCTGCACCG CCAAGCGAAG 
CTCAACCTCA AATCAGTGCT GAGGGAATGT GACAAGCTGA GGCGCGATGG CTGCCGAAGT 
TCTCAGTACT ATTCTCAGGG ACCCACCTTT GCAGCCAGCT CCAGCCCAGG CGATGACTAT 
CAAGAGGAAG ATGCAGAAAC AGACCGAAAG TATTCTCTCT CTTCATCTGA AGAAGAAAGA 
TTTATTGGTA TCAGGAGACC TAAAACGCCA ACCTCAGGTG ACTTCTCTGA CCTTCATACT 
CAGACAAACT GGACCAAGTC CCTGCCGCTG CCAACACCAG AAGAGAAGAC ACGACAGCAA 
GCCCAGACAG TCCAGGCTGA TGTGGTTCCT ATTAACATAA CTGGGGAGAA TTTTGATCGT 
CAGGCCAGCC TTCGGCGGTC TCTAATTTAC ACTGACACTC TGGTAAGACG ACCGAAGAAA 
GTCAAAAGGA GAAAGACTAT TTCAGGAATC CCTGACATCA TTCAGAAGGA GCTAGCGTCA 
GCCACGGGCC AAGAGGATGA TGGTAGCACC CATTCTCTAT ACTCCCCAGA CCACTACTCT 
ACATTAGGAA GGCTTGATAG CTATCGGTCC ACGGGGCAAT GCTTGGAAAC CAGGGACTCT 
GGCTGTCAGA CAGAAGAAGT GAAAGTCATC CCACCGTCAG TGAGAAGGAT CAGGGCTCAC 
AAGGGGCTGG GCATTGCTGC CCAGATGAGC CACCTCTCGG GTTCCTCTGG CAACATGTCT 
GTGCTGAGCG ACTCGGCTGG TATCGTGTTC CCATCTCGCC TCAATAACGA TACTGGTTTC 
CACAGCCTTC CACGCGCTGG CGCAAGGGCA AGCACGTATT CCCTGGAGGC AAGGATGGGT 
GCCCTGGGCT CTGCTGAAGA TACGGATGAT ACTTTTCCCT ACCGAGGGGG TAGCTCCCAG 
GGGCCTGAAA ACTTTGCACA TTTAGGGGGT GCCTCGAGTA CCGGGATGCT TTCGAGGCCC 
AAATCCCAGC AGCTGAGGCT CTTGGAGAGC CCAGCGTGTG TGGTTTCACC GCACGCGGCC 
TACTCTACCA GCGTCATCCC AAACGCCACA CTGCTGTCCT CTTCGGAGGT TATTGTCATC 
CACACCGCTC AGAGTGCAGG ACAGCTGGAC AGTAGAGTCC CCGGCTCATC CTCATACTCA 
AAGATAAAAC CCAGAGACCA TCCCACACCC AGGTGCTCTG CGAGAGATGA GCGCCAGTCC 
CCACGACATC ACTGGAACGA GGGTCATATC ATTCACTCAC AGGCTTTAGC CTCCGCTGCC 
CCCGGTGCCA CCGCACTGTT ATCCCTCCGT GACTCAGAGG TCTCTCTCAA TGCCCCAGCT 
ATCAATAGGG AGAACGGGTC CCAAGCCATA CTTTATCACT GTAGAAGCAA CCTGGCCTTT 
CCTGCCCACC CTCCGGATGT GGATGGCAAG AGCGAGTGCA GTTCTTCAGG GGACAGGGGA 
TGTGGCAGTT CTGAGTCCTG GGAATACAAA GCCTCCAGCA ATGGGCGGGC TTCCCCACTG 
AAGCCGCACC TTGCGACTCC CGGTTGCTCT ACCCCCACAA GTAACATGAG CAGCTGCAGC 
TTGGACCAAG CATCCCTCAA GGAGGATGCC AGGTCCCTGT ATTCAGAGGA CCACGATGGT 
TACTATATGA CCGCCCAGGG TGATTCTAGA CACGAGGCTG GGAGCGCGTA CGGTATCAGT 
GATGGCTTTG GAAACCCCAG GCACAGCACG GTCAATGTTT TTGACGGAAG GGCTCAGAGA 
AGCCAAGGGG ATCAGGCCAC TCAGCAGGAT AGAATCCTCT CAAGAAACAT CTCTCTGAAG 
AAAGCAAAAA AACCACCACC GCCACCATCC CGGACAGATT CTCTGCGAAG GATTCCCAAG 
AAGAACAACC AGACAAATGG GCAGGTGCTC AACGAGAGCC TGATCGCCTC ACTCCAGCAT 
TCTCTGCAGC TAAGCCTCCC TGGCCAAGGC AGCGGTTCCC CTTCCCAGAG CCCCTGCAGT 
GACTGTGAGG AACCCTGGCT CCCCAGGTCC AGAAGCCAGA GCATTGTTAG CGAGGGTAGT 
AGCTTGACCT CCACCACCAC TCCCAACGTG TATTCTCTGT GCGGGCTCAC TCCATCTCAG 
AGCGACACAA GCAGCGTCAA GTCGGAATAC GCAGACCCCT GGGGCTACTA CATTGACTAC 
ACAACTTTGC AGGGAGATCC AGGGAACCCC ACAGGGGGCT GCCCAGCCAG TAGTGAGGCA 
GCCACTGGAA ATGGGCCAGT GCGCCACATC CAGGAGGGGT CGAGAGCCCC AGTGCCCCAA 
GTGCCGGGCT GCTCCGTGAA ACCAAAGATC ACCTCACCGG AGAAGTCACA GAGAGTCACC 
TCTCCATCCA GTGGGTATTC TAGCCAGTCG AATACACCCA CAGCACTCAC CCCCGTGCCT 
GTGTTTTTAA AATCGATGTC ACCAGCAAAT GGGAAGGGGA AGGCCAAGCC CAAGGTACCA 
GAAAGAAAAT CGTCTCTGAT CTCTTCAGTG TCCATCTCCT CATCGTCCAC TTCCCTCTCC 
TCTAATACCT CTACGGAAGG AAGTGGGACC GTGAAGAAAC TGGATTCAGC CCTGGCCTCG 
GCCCTTGCTC CTCCCCCTCC TCCTCTTCCC GCGCTGCCTT CTCCGAGTCT GGCAAACAAG 
TCCCCCTTCC TTCCACCTCC TCCTCCTCTA GCAGATTGCT CCGAGGGCTC TCCCTTACCG 
CCCTCTCCTG TGTTCCCCCC TCCACCACCA GAAGCCCTTG TTCCCTTCTG CTCCCCCACT 
GACAGGTGCC TTTCTCCTTC CCCCACAGCT TTTAGCCCCC CTCTTCCAAG ATGCTCACCT 
CCCTTGCCAG CACCTCCACC TTTCTTGCCC CCATCAGAAC CCCCACCTGC TCCGCCTCTG 
GACCCCAAAT TCATGAAAGA AAACAGGCCC TTTATCAAAA ACTCTAGCCA GTCCGAATCC 
TCTAGGGAGG CCCTCAGGCG GCCTGCCAAC AAGGAGGAGG GCTGCCGACC CTCGATGCCC 
CTGATCACCA CAGAAGCTTT GCAGATGGTG CAGTTGAGGC CAGTGAGGAA GAACTCAGGT 
GCTGAGACGG TCCTGTTTTC TGAACCATCA GCTCAGGAAC CACGAACCCC GACTGCTCCA 
CAGTATCACT TAAAGCCATC TGCTTTCCTC AAATCCCGAA ATAGCATAAA TGAAATGGAG 
AGTGAAAGCC AGGCTGCCTC TGTGACGAGC TCGCTTCCGA TGCCTGCCAA GAGCCAGAGT 
CAGGGTGACC ATGACAGTGC AGTCGAGCGT GGCGGCCTTC AGAGCTGCAG CGATGGAGCT 
CCAGGCCCGG GTCCCAGCCT CAGGACCGCT CTGCTCCCAG ACTCTTCACC CAGCAGGAAG 
CCACCCCCCA TCTCCAAGAA GCCCAAACTG TTCCTGGTGG TACCACCTCC GCAGAGAGAT 
TTCACAGCGG AGCCCACAGA GAACGGGAGC GAAGCTTTCT CAGGCGTGCC CAGCCCTACC 
GGGGCAGAGG GGGAAGCTGG GCAGAGCCAA GAGGAGAAAT CCAGCCCAGC ATCCCAAGCT 
GGCTCTCATG CTACGGCCCC CACCTCCGGC AGTACGGTTC TAGAAAGAGG AACTGCAGGG 
TCCTTGTCCC CTGGCGTTGT GGAAGCTAAT GCCCCCATGG TCCAACCTAG TACATCACCG 
GGCCCCGCAC AGGAAGAGGC AGGAGAGAAC CGCGTGGATG GTGAGAGAAA TGTGAAGAGC 
TGCGCGTCTC CACGGGGCGG AGAAGCTGGG TTGCCGGAAC CCAACACAGC TGGTCCGTCT 
TCAGACACGG CAGACGTTTC TAAAGAAGGA GGGAACGATG AGGTGCTGAC CCCTACCAGA 
CCCAGGACAA CGGAAGACCT GTTTGCAGCC ATTCACAGAT CCAAAAGGAA AGTCCTTGGT 
CGGAAGGACT CCGAAGATGA TCACACTCGA AATCATTCTC CCTCCCCGCC TGTGACACCC 
ACAGGTGCTG CCCCTAGCCT GGCCTCTCCA AAGCAAGTAG GGTCCATCCA GCGAAGCCTA 
AAAAAGAGCA GTACCAGCAG CGACAACTTC AAAGCTCTGC TGCTCAAGAA GGGGAGTCGC 
TCGGACACCA GCGCTCGCAT GTCCGCGGCG GAGATGCTCA AGTCCACGGA CCCTCGATTC 
CAGAGATCTA GGTCGGAGCC TTCGCCAGAG GTCCCGGACA GCCCATCCAG CTTCTCTCCC 
AATAAGAACA GGAGAGCTCC GGAGGAGTGG GCCAAGAATG AAGGACTGAT GCCTCGGAGT 
CTGTCCTTTT CCGGCCCTCG GTACAGCCGC AGCAGGACGC CACCCTCTGC GGCCAGCAGC 
AGGTACAGCA TGAGGAACCG CATCCAAAGC AGCCCCATGA CAGTCATCTC TGAGGGAGAG 
GGAGAGCCTG CCGAGCCCGC AGACAACAAA GTACGCAGGA CCCTGGACAC TACGAGGGGA 
TGCTCTCTGG ACAGACTGGC GGGACAGGAG ACGGACCGGG GCAGCCCGCT GTGTAGCGAG 
GAGCCTGCCT CTGTGGATGG GATAGGAAGG GCTAAGGACG ACGGTCCCTC GGAGCAGTGT 
AGGGGTCCTG AACAGAAGAG TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nhsl1 ( XM_017589823.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017445312.1 Rattus norvegicus NHS-like 1 (Nhsl1), transcript variant X1, mRNA.

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CloneID	ORa53614
Clone ID Related Accession (Same CDS sequence)	XM_017587917.1 , XM_017589823.1
Accession Version	XM_017589823.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5004bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	NHS-like protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCTTCC ACCAACGGAG CGTGGAGCCC GCACGGCTGC GCCGGCCCGA GGAGGCGGCG 
GGGGCTCTGG CTGGGGACCC GCCGCTCTTC CGCTCGCTGG AGCAGGTCAG CTCGCACACC 
CTGGTCTGCT TGCTCGCCCA GCTGGCCGAC CTGTCGCGCT GCGCTGGGGA CATTTTCGGG 
GAGCTCGAGG GCCAGGCGGC CGCGCTGGGT CACCGCACCG CCGAGTTGCA CCGTCGCCTG 
GACGCCCTGC ACGCTGCCGC CGCGCGCCTG GACCACCGGC GAGTGAAAAT CCCGGTTTCC 
AACTTAGACG AGGAGAGCCG ATGGACCGTC CACTATACGG CGCCGTGGCA CCAGCAGGAG 
AATGTGTTCC TCCCCTCCAC AAGACCCCCC TGTGTTGAGG ACCTGCACCG CCAAGCGAAG 
CTCAACCTCA AATCAGTGCT GAGGGAATGT GACAAGCTGA GGCGCGATGG CTGCCGAAGT 
TCTCAGTACT ATTCTCAGGG ACCCACCTTT GCAGCCAGCT CCAGCCCAGG CGATGACTAT 
CAAGAGGAAG ATGCAGAAAC AGACCGAAAG TATTCTCTCT CTTCATCTGA AGAAGAAAGA 
TTTATTGGTA TCAGGAGACC TAAAACGCCA ACCTCAGGTG ACTTCTCTGA CCTTCATACT 
CAGACAAACT GGACCAAGTC CCTGCCGCTG CCAACACCAG AAGAGAAGAC ACGACAGCAA 
GCCCAGACAG TCCAGGCTGA TGTGGTTCCT ATTAACATAA CTGGGGAGAA TTTTGATCGT 
CAGGCCAGCC TTCGGCGGTC TCTAATTTAC ACTGACACTC TGGTAAGACG ACCGAAGAAA 
GTCAAAAGGA GAAAGACTAT TTCAGGAATC CCTGACATCA TTCAGAAGGA GCTAGCGTCA 
GCCACGGGCC AAGAGGATGA TGGTAGCACC CATTCTCTAT ACTCCCCAGA CCACTACTCT 
ACATTAGGAA GGCTTGATAG CTATCGGTCC ACGGGGCAAT GCTTGGAAAC CAGGGACTCT 
GGCTGTCAGA CAGAAGAAGT GAAAGTCATC CCACCGTCAG TGAGAAGGAT CAGGGCTCAC 
AAGGGGCTGG GCATTGCTGC CCAGATGAGC CACCTCTCGG GTTCCTCTGG CAACATGTCT 
GTGCTGAGCG ACTCGGCTGG TATCGTGTTC CCATCTCGCC TCAATAACGA TACTGGTTTC 
CACAGCCTTC CACGCGCTGG CGCAAGGGCA AGCACGTATT CCCTGGAGGC AAGGATGGGT 
GCCCTGGGCT CTGCTGAAGA TACGGATGAT ACTTTTCCCT ACCGAGGGGG TAGCTCCCAG 
GGGCCTGAAA ACTTTGCACA TTTAGGGGGT GCCTCGAGTA CCGGGATGCT TTCGAGGCCC 
AAATCCCAGC AGCTGAGGCT CTTGGAGAGC CCAGCGTGTG TGGTTTCACC GCACGCGGCC 
TACTCTACCA GCGTCATCCC AAACGCCACA CTGCTGTCCT CTTCGGAGGT TATTGTCATC 
CACACCGCTC AGAGTGCAGG ACAGCTGGAC AGTAGAGTCC CCGGCTCATC CTCATACTCA 
AAGATAAAAC CCAGAGACCA TCCCACACCC AGGTGCTCTG CGAGAGATGA GCGCCAGTCC 
CCACGACATC ACTGGAACGA GGGTCATATC ATTCACTCAC AGGCTTTAGC CTCCGCTGCC 
CCCGGTGCCA CCGCACTGTT ATCCCTCCGT GACTCAGAGG TCTCTCTCAA TGCCCCAGCT 
ATCAATAGGG AGAACGGGTC CCAAGCCATA CTTTATCACT GTAGAAGCAA CCTGGCCTTT 
CCTGCCCACC CTCCGGATGT GGATGGCAAG AGCGAGTGCA GTTCTTCAGG GGACAGGGGA 
TGTGGCAGTT CTGAGTCCTG GGAATACAAA GCCTCCAGCA ATGGGCGGGC TTCCCCACTG 
AAGCCGCACC TTGCGACTCC CGGTTGCTCT ACCCCCACAA GTAACATGAG CAGCTGCAGC 
TTGGACCAAG CATCCCTCAA GGAGGATGCC AGGTCCCTGT ATTCAGAGGA CCACGATGGT 
TACTATATGA CCGCCCAGGG TGATTCTAGA CACGAGGCTG GGAGCGCGTA CGGTATCAGT 
GATGGCTTTG GAAACCCCAG GCACAGCACG GTCAATGTTT TTGACGGAAG GGCTCAGAGA 
AGCCAAGGGG ATCAGGCCAC TCAGCAGGAT AGAATCCTCT CAAGAAACAT CTCTCTGAAG 
AAAGCAAAAA AACCACCACC GCCACCATCC CGGACAGATT CTCTGCGAAG GATTCCCAAG 
AAGAACAACC AGACAAATGG GCAGGTGCTC AACGAGAGCC TGATCGCCTC ACTCCAGCAT 
TCTCTGCAGC TAAGCCTCCC TGGCCAAGGC AGCGGTTCCC CTTCCCAGAG CCCCTGCAGT 
GACTGTGAGG AACCCTGGCT CCCCAGGTCC AGAAGCCAGA GCATTGTTAG CGAGGGTAGT 
AGCTTGACCT CCACCACCAC TCCCAACGTG TATTCTCTGT GCGGGCTCAC TCCATCTCAG 
AGCGACACAA GCAGCGTCAA GTCGGAATAC GCAGACCCCT GGGGCTACTA CATTGACTAC 
ACAACTTTGC AGGGAGATCC AGGGAACCCC ACAGGGGGCT GCCCAGCCAG TAGTGAGGCA 
GCCACTGGAA ATGGGCCAGT GCGCCACATC CAGGAGGGGT CGAGAGCCCC AGTGCCCCAA 
GTGCCGGGCT GCTCCGTGAA ACCAAAGATC ACCTCACCGG AGAAGTCACA GAGAGTCACC 
TCTCCATCCA GTGGGTATTC TAGCCAGTCG AATACACCCA CAGCACTCAC CCCCGTGCCT 
GTGTTTTTAA AATCGATGTC ACCAGCAAAT GGGAAGGGGA AGGCCAAGCC CAAGGTACCA 
GAAAGAAAAT CGTCTCTGAT CTCTTCAGTG TCCATCTCCT CATCGTCCAC TTCCCTCTCC 
TCTAATACCT CTACGGAAGG AAGTGGGACC GTGAAGAAAC TGGATTCAGC CCTGGCCTCG 
GCCCTTGCTC CTCCCCCTCC TCCTCTTCCC GCGCTGCCTT CTCCGAGTCT GGCAAACAAG 
TCCCCCTTCC TTCCACCTCC TCCTCCTCTA GCAGATTGCT CCGAGGGCTC TCCCTTACCG 
CCCTCTCCTG TGTTCCCCCC TCCACCACCA GAAGCCCTTG TTCCCTTCTG CTCCCCCACT 
GACAGGTGCC TTTCTCCTTC CCCCACAGCT TTTAGCCCCC CTCTTCCAAG ATGCTCACCT 
CCCTTGCCAG CACCTCCACC TTTCTTGCCC CCATCAGAAC CCCCACCTGC TCCGCCTCTG 
GACCCCAAAT TCATGAAAGA AAACAGGCCC TTTATCAAAA ACTCTAGCCA GTCCGAATCC 
TCTAGGGAGG CCCTCAGGCG GCCTGCCAAC AAGGAGGAGG GCTGCCGACC CTCGATGCCC 
CTGATCACCA CAGAAGCTTT GCAGATGGTG CAGTTGAGGC CAGTGAGGAA GAACTCAGGT 
GCTGAGACGG TCCTGTTTTC TGAACCATCA GCTCAGGAAC CACGAACCCC GACTGCTCCA 
CAGTATCACT TAAAGCCATC TGCTTTCCTC AAATCCCGAA ATAGCATAAA TGAAATGGAG 
AGTGAAAGCC AGGCTGCCTC TGTGACGAGC TCGCTTCCGA TGCCTGCCAA GAGCCAGAGT 
CAGGGTGACC ATGACAGTGC AGTCGAGCGT GGCGGCCTTC AGAGCTGCAG CGATGGAGCT 
CCAGGCCCGG GTCCCAGCCT CAGGACCGCT CTGCTCCCAG ACTCTTCACC CAGCAGGAAG 
CCACCCCCCA TCTCCAAGAA GCCCAAACTG TTCCTGGTGG TACCACCTCC GCAGAGAGAT 
TTCACAGCGG AGCCCACAGA GAACGGGAGC GAAGCTTTCT CAGGCGTGCC CAGCCCTACC 
GGGGCAGAGG GGGAAGCTGG GCAGAGCCAA GAGGAGAAAT CCAGCCCAGC ATCCCAAGCT 
GGCTCTCATG CTACGGCCCC CACCTCCGGC AGTACGGTTC TAGAAAGAGG AACTGCAGGG 
TCCTTGTCCC CTGGCGTTGT GGAAGCTAAT GCCCCCATGG TCCAACCTAG TACATCACCG 
GGCCCCGCAC AGGAAGAGGC AGGAGAGAAC CGCGTGGATG GTGAGAGAAA TGTGAAGAGC 
TGCGCGTCTC CACGGGGCGG AGAAGCTGGG TTGCCGGAAC CCAACACAGC TGGTCCGTCT 
TCAGACACGG CAGACGTTTC TAAAGAAGGA GGGAACGATG AGGTGCTGAC CCCTACCAGA 
CCCAGGACAA CGGAAGACCT GTTTGCAGCC ATTCACAGAT CCAAAAGGAA AGTCCTTGGT 
CGGAAGGACT CCGAAGATGA TCACACTCGA AATCATTCTC CCTCCCCGCC TGTGACACCC 
ACAGGTGCTG CCCCTAGCCT GGCCTCTCCA AAGCAAGTAG GGTCCATCCA GCGAAGCCTA 
AAAAAGAGCA GTACCAGCAG CGACAACTTC AAAGCTCTGC TGCTCAAGAA GGGGAGTCGC 
TCGGACACCA GCGCTCGCAT GTCCGCGGCG GAGATGCTCA AGTCCACGGA CCCTCGATTC 
CAGAGATCTA GGTCGGAGCC TTCGCCAGAG GTCCCGGACA GCCCATCCAG CTTCTCTCCC 
AATAAGAACA GGAGAGCTCC GGAGGAGTGG GCCAAGAATG AAGGACTGAT GCCTCGGAGT 
CTGTCCTTTT CCGGCCCTCG GTACAGCCGC AGCAGGACGC CACCCTCTGC GGCCAGCAGC 
AGGTACAGCA TGAGGAACCG CATCCAAAGC AGCCCCATGA CAGTCATCTC TGAGGGAGAG 
GGAGAGCCTG CCGAGCCCGC AGACAACAAA GTACGCAGGA CCCTGGACAC TACGAGGGGA 
TGCTCTCTGG ACAGACTGGC GGGACAGGAG ACGGACCGGG GCAGCCCGCT GTGTAGCGAG 
GAGCCTGCCT CTGTGGATGG GATAGGAAGG GCTAAGGACG ACGGTCCCTC GGAGCAGTGT 
AGGGGTCCTG AACAGAAGAG TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017445312.1
CDS	288..5291
Translation	MPFHQRSVEPARLRRPEEAAGALAGDPPLFRSLEQVSSHTLVCLLAQLADLSRCAGDIFGELEGQAAALGHRTAELHRRLDALHAAAARLDHRRVKIPVSNLDEESRWTVHYTAPWHQQENVFLPSTRPPCVEDLHRQAKLNLKSVLRECDKLRRDGCRSSQYYSQGPTFAASSSPGDDYQEEDAETDRKYSLSSSEEERFIGIRRPKTPTSGDFSDLHTQTNWTKSLPLPTPEEKTRQQAQTVQADVVPINITGENFDRQASLRRSLIYTDTLVRRPKKVKRRKTISGIPDIIQKELASATGQEDDGSTHSLYSPDHYSTLGRLDSYRSTGQCLETRDSGCQTEEVKVIPPSVRRIRAHKGLGIAAQMSHLSGSSGNMSVLSDSAGIVFPSRLNNDTGFHSLPRAGARASTYSLEARMGALGSAEDTDDTFPYRGGSSQGPENFAHLGGASSTGMLSRPKSQQLRLLESPACVVSPHAAYSTSVIPNATLLSSSEVIVIHTAQSAGQLDSRVPGSSSYSKIKPRDHPTPRCSARDERQSPRHHWNEGHIIHSQALASAAPGATALLSLRDSEVSLNAPAINRENGSQAILYHCRSNLAFPAHPPDVDGKSECSSSGDRGCGSSESWEYKASSNGRASPLKPHLATPGCSTPTSNMSSCSLDQASLKEDARSLYSEDHDGYYMTAQGDSRHEAGSAYGISDGFGNPRHSTVNVFDGRAQRSQGDQATQQDRILSRNISLKKAKKPPPPPSRTDSLRRIPKKNNQTNGQVLNESLIASLQHSLQLSLPGQGSGSPSQSPCSDCEEPWLPRSRSQSIVSEGSSLTSTTTPNVYSLCGLTPSQSDTSSVKSEYADPWGYYIDYTTLQGDPGNPTGGCPASSEAATGNGPVRHIQEGSRAPVPQVPGCSVKPKITSPEKSQRVTSPSSGYSSQSNTPTALTPVPVFLKSMSPANGKGKAKPKVPERKSSLISSVSISSSSTSLSSNTSTEGSGTVKKLDSALASALAPPPPPLPALPSPSLANKSPFLPPPPPLADCSEGSPLPPSPVFPPPPPEALVPFCSPTDRCLSPSPTAFSPPLPRCSPPLPAPPPFLPPSEPPPAPPLDPKFMKENRPFIKNSSQSESSREALRRPANKEEGCRPSMPLITTEALQMVQLRPVRKNSGAETVLFSEPSAQEPRTPTAPQYHLKPSAFLKSRNSINEMESESQAASVTSSLPMPAKSQSQGDHDSAVERGGLQSCSDGAPGPGPSLRTALLPDSSPSRKPPPISKKPKLFLVVPPPQRDFTAEPTENGSEAFSGVPSPTGAEGEAGQSQEEKSSPASQAGSHATAPTSGSTVLERGTAGSLSPGVVEANAPMVQPSTSPGPAQEEAGENRVDGERNVKSCASPRGGEAGLPEPNTAGPSSDTADVSKEGGNDEVLTPTRPRTTEDLFAAIHRSKRKVLGRKDSEDDHTRNHSPSPPVTPTGAAPSLASPKQVGSIQRSLKKSSTSSDNFKALLLKKGSRSDTSARMSAAEMLKSTDPRFQRSRSEPSPEVPDSPSSFSPNKNRRAPEEWAKNEGLMPRSLSFSGPRYSRSRTPPSAASSRYSMRNRIQSSPMTVISEGEGEPAEPADNKVRRTLDTTRGCSLDRLAGQETDRGSPLCSEEPASVDGIGRAKDDGPSEQCRGPEQKS

Target ORF information:

RefSeq Version	XM_017589823.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus NHS-like 1 (Nhsl1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017589823.1

ORF Insert Sequence:

ATGCCCTTCC ACCAACGGAG CGTGGAGCCC GCACGGCTGC GCCGGCCCGA GGAGGCGGCG 
GGGGCTCTGG CTGGGGACCC GCCGCTCTTC CGCTCGCTGG AGCAGGTCAG CTCGCACACC 
CTGGTCTGCT TGCTCGCCCA GCTGGCCGAC CTGTCGCGCT GCGCTGGGGA CATTTTCGGG 
GAGCTCGAGG GCCAGGCGGC CGCGCTGGGT CACCGCACCG CCGAGTTGCA CCGTCGCCTG 
GACGCCCTGC ACGCTGCCGC CGCGCGCCTG GACCACCGGC GAGTGAAAAT CCCGGTTTCC 
AACTTAGACG AGGAGAGCCG ATGGACCGTC CACTATACGG CGCCGTGGCA CCAGCAGGAG 
AATGTGTTCC TCCCCTCCAC AAGACCCCCC TGTGTTGAGG ACCTGCACCG CCAAGCGAAG 
CTCAACCTCA AATCAGTGCT GAGGGAATGT GACAAGCTGA GGCGCGATGG CTGCCGAAGT 
TCTCAGTACT ATTCTCAGGG ACCCACCTTT GCAGCCAGCT CCAGCCCAGG CGATGACTAT 
CAAGAGGAAG ATGCAGAAAC AGACCGAAAG TATTCTCTCT CTTCATCTGA AGAAGAAAGA 
TTTATTGGTA TCAGGAGACC TAAAACGCCA ACCTCAGGTG ACTTCTCTGA CCTTCATACT 
CAGACAAACT GGACCAAGTC CCTGCCGCTG CCAACACCAG AAGAGAAGAC ACGACAGCAA 
GCCCAGACAG TCCAGGCTGA TGTGGTTCCT ATTAACATAA CTGGGGAGAA TTTTGATCGT 
CAGGCCAGCC TTCGGCGGTC TCTAATTTAC ACTGACACTC TGGTAAGACG ACCGAAGAAA 
GTCAAAAGGA GAAAGACTAT TTCAGGAATC CCTGACATCA TTCAGAAGGA GCTAGCGTCA 
GCCACGGGCC AAGAGGATGA TGGTAGCACC CATTCTCTAT ACTCCCCAGA CCACTACTCT 
ACATTAGGAA GGCTTGATAG CTATCGGTCC ACGGGGCAAT GCTTGGAAAC CAGGGACTCT 
GGCTGTCAGA CAGAAGAAGT GAAAGTCATC CCACCGTCAG TGAGAAGGAT CAGGGCTCAC 
AAGGGGCTGG GCATTGCTGC CCAGATGAGC CACCTCTCGG GTTCCTCTGG CAACATGTCT 
GTGCTGAGCG ACTCGGCTGG TATCGTGTTC CCATCTCGCC TCAATAACGA TACTGGTTTC 
CACAGCCTTC CACGCGCTGG CGCAAGGGCA AGCACGTATT CCCTGGAGGC AAGGATGGGT 
GCCCTGGGCT CTGCTGAAGA TACGGATGAT ACTTTTCCCT ACCGAGGGGG TAGCTCCCAG 
GGGCCTGAAA ACTTTGCACA TTTAGGGGGT GCCTCGAGTA CCGGGATGCT TTCGAGGCCC 
AAATCCCAGC AGCTGAGGCT CTTGGAGAGC CCAGCGTGTG TGGTTTCACC GCACGCGGCC 
TACTCTACCA GCGTCATCCC AAACGCCACA CTGCTGTCCT CTTCGGAGGT TATTGTCATC 
CACACCGCTC AGAGTGCAGG ACAGCTGGAC AGTAGAGTCC CCGGCTCATC CTCATACTCA 
AAGATAAAAC CCAGAGACCA TCCCACACCC AGGTGCTCTG CGAGAGATGA GCGCCAGTCC 
CCACGACATC ACTGGAACGA GGGTCATATC ATTCACTCAC AGGCTTTAGC CTCCGCTGCC 
CCCGGTGCCA CCGCACTGTT ATCCCTCCGT GACTCAGAGG TCTCTCTCAA TGCCCCAGCT 
ATCAATAGGG AGAACGGGTC CCAAGCCATA CTTTATCACT GTAGAAGCAA CCTGGCCTTT 
CCTGCCCACC CTCCGGATGT GGATGGCAAG AGCGAGTGCA GTTCTTCAGG GGACAGGGGA 
TGTGGCAGTT CTGAGTCCTG GGAATACAAA GCCTCCAGCA ATGGGCGGGC TTCCCCACTG 
AAGCCGCACC TTGCGACTCC CGGTTGCTCT ACCCCCACAA GTAACATGAG CAGCTGCAGC 
TTGGACCAAG CATCCCTCAA GGAGGATGCC AGGTCCCTGT ATTCAGAGGA CCACGATGGT 
TACTATATGA CCGCCCAGGG TGATTCTAGA CACGAGGCTG GGAGCGCGTA CGGTATCAGT 
GATGGCTTTG GAAACCCCAG GCACAGCACG GTCAATGTTT TTGACGGAAG GGCTCAGAGA 
AGCCAAGGGG ATCAGGCCAC TCAGCAGGAT AGAATCCTCT CAAGAAACAT CTCTCTGAAG 
AAAGCAAAAA AACCACCACC GCCACCATCC CGGACAGATT CTCTGCGAAG GATTCCCAAG 
AAGAACAACC AGACAAATGG GCAGGTGCTC AACGAGAGCC TGATCGCCTC ACTCCAGCAT 
TCTCTGCAGC TAAGCCTCCC TGGCCAAGGC AGCGGTTCCC CTTCCCAGAG CCCCTGCAGT 
GACTGTGAGG AACCCTGGCT CCCCAGGTCC AGAAGCCAGA GCATTGTTAG CGAGGGTAGT 
AGCTTGACCT CCACCACCAC TCCCAACGTG TATTCTCTGT GCGGGCTCAC TCCATCTCAG 
AGCGACACAA GCAGCGTCAA GTCGGAATAC GCAGACCCCT GGGGCTACTA CATTGACTAC 
ACAACTTTGC AGGGAGATCC AGGGAACCCC ACAGGGGGCT GCCCAGCCAG TAGTGAGGCA 
GCCACTGGAA ATGGGCCAGT GCGCCACATC CAGGAGGGGT CGAGAGCCCC AGTGCCCCAA 
GTGCCGGGCT GCTCCGTGAA ACCAAAGATC ACCTCACCGG AGAAGTCACA GAGAGTCACC 
TCTCCATCCA GTGGGTATTC TAGCCAGTCG AATACACCCA CAGCACTCAC CCCCGTGCCT 
GTGTTTTTAA AATCGATGTC ACCAGCAAAT GGGAAGGGGA AGGCCAAGCC CAAGGTACCA 
GAAAGAAAAT CGTCTCTGAT CTCTTCAGTG TCCATCTCCT CATCGTCCAC TTCCCTCTCC 
TCTAATACCT CTACGGAAGG AAGTGGGACC GTGAAGAAAC TGGATTCAGC CCTGGCCTCG 
GCCCTTGCTC CTCCCCCTCC TCCTCTTCCC GCGCTGCCTT CTCCGAGTCT GGCAAACAAG 
TCCCCCTTCC TTCCACCTCC TCCTCCTCTA GCAGATTGCT CCGAGGGCTC TCCCTTACCG 
CCCTCTCCTG TGTTCCCCCC TCCACCACCA GAAGCCCTTG TTCCCTTCTG CTCCCCCACT 
GACAGGTGCC TTTCTCCTTC CCCCACAGCT TTTAGCCCCC CTCTTCCAAG ATGCTCACCT 
CCCTTGCCAG CACCTCCACC TTTCTTGCCC CCATCAGAAC CCCCACCTGC TCCGCCTCTG 
GACCCCAAAT TCATGAAAGA AAACAGGCCC TTTATCAAAA ACTCTAGCCA GTCCGAATCC 
TCTAGGGAGG CCCTCAGGCG GCCTGCCAAC AAGGAGGAGG GCTGCCGACC CTCGATGCCC 
CTGATCACCA CAGAAGCTTT GCAGATGGTG CAGTTGAGGC CAGTGAGGAA GAACTCAGGT 
GCTGAGACGG TCCTGTTTTC TGAACCATCA GCTCAGGAAC CACGAACCCC GACTGCTCCA 
CAGTATCACT TAAAGCCATC TGCTTTCCTC AAATCCCGAA ATAGCATAAA TGAAATGGAG 
AGTGAAAGCC AGGCTGCCTC TGTGACGAGC TCGCTTCCGA TGCCTGCCAA GAGCCAGAGT 
CAGGGTGACC ATGACAGTGC AGTCGAGCGT GGCGGCCTTC AGAGCTGCAG CGATGGAGCT 
CCAGGCCCGG GTCCCAGCCT CAGGACCGCT CTGCTCCCAG ACTCTTCACC CAGCAGGAAG 
CCACCCCCCA TCTCCAAGAA GCCCAAACTG TTCCTGGTGG TACCACCTCC GCAGAGAGAT 
TTCACAGCGG AGCCCACAGA GAACGGGAGC GAAGCTTTCT CAGGCGTGCC CAGCCCTACC 
GGGGCAGAGG GGGAAGCTGG GCAGAGCCAA GAGGAGAAAT CCAGCCCAGC ATCCCAAGCT 
GGCTCTCATG CTACGGCCCC CACCTCCGGC AGTACGGTTC TAGAAAGAGG AACTGCAGGG 
TCCTTGTCCC CTGGCGTTGT GGAAGCTAAT GCCCCCATGG TCCAACCTAG TACATCACCG 
GGCCCCGCAC AGGAAGAGGC AGGAGAGAAC CGCGTGGATG GTGAGAGAAA TGTGAAGAGC 
TGCGCGTCTC CACGGGGCGG AGAAGCTGGG TTGCCGGAAC CCAACACAGC TGGTCCGTCT 
TCAGACACGG CAGACGTTTC TAAAGAAGGA GGGAACGATG AGGTGCTGAC CCCTACCAGA 
CCCAGGACAA CGGAAGACCT GTTTGCAGCC ATTCACAGAT CCAAAAGGAA AGTCCTTGGT 
CGGAAGGACT CCGAAGATGA TCACACTCGA AATCATTCTC CCTCCCCGCC TGTGACACCC 
ACAGGTGCTG CCCCTAGCCT GGCCTCTCCA AAGCAAGTAG GGTCCATCCA GCGAAGCCTA 
AAAAAGAGCA GTACCAGCAG CGACAACTTC AAAGCTCTGC TGCTCAAGAA GGGGAGTCGC 
TCGGACACCA GCGCTCGCAT GTCCGCGGCG GAGATGCTCA AGTCCACGGA CCCTCGATTC 
CAGAGATCTA GGTCGGAGCC TTCGCCAGAG GTCCCGGACA GCCCATCCAG CTTCTCTCCC 
AATAAGAACA GGAGAGCTCC GGAGGAGTGG GCCAAGAATG AAGGACTGAT GCCTCGGAGT 
CTGTCCTTTT CCGGCCCTCG GTACAGCCGC AGCAGGACGC CACCCTCTGC GGCCAGCAGC 
AGGTACAGCA TGAGGAACCG CATCCAAAGC AGCCCCATGA CAGTCATCTC TGAGGGAGAG 
GGAGAGCCTG CCGAGCCCGC AGACAACAAA GTACGCAGGA CCCTGGACAC TACGAGGGGA 
TGCTCTCTGG ACAGACTGGC GGGACAGGAG ACGGACCGGG GCAGCCCGCT GTGTAGCGAG 
GAGCCTGCCT CTGTGGATGG GATAGGAAGG GCTAAGGACG ACGGTCCCTC GGAGCAGTGT 
AGGGGTCCTG AACAGAAGAG TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nhsl1 ( XM_017587918.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017443407.1 Rattus norvegicus NHS-like 1 (Nhsl1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ORa53615
Clone ID Related Accession (Same CDS sequence)	XM_017587918.1 , XM_017589824.1
Accession Version	XM_017587918.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4872bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	NHS-like protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (AC_000069.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCTTCC ACCAACGGAG CGTGGAGCCC GCACGGCTGC GCCGGCCCGA GGAGGCGGCG 
GGGGCTCTGG CTGGGGACCC GCCGCTCTTC CGCTCGCTGG AGCAGGTCAG CTCGCACACC 
CTGGTCTGCT TGCTCGCCCA GCTGGCCGAC CTGTCGCGCT GCGCTGGGGA CATTTTCGGG 
GAGCTCGAGG GCCAGGCGGC CGCGCTGGGT CACCGCACCG CCGAGTTGCA CCGTCGCCTG 
GACGCCCTGC ACGCTGCCGC CGCGCGCCTG GACCACCGGC GAGTGAAAAT CCCGGTTTCC 
AACTTAGACG AGGAGAGCCG ATGGACCGTC CACTATACGG CGCCGTGGCA CCAGCAGGAG 
AATGTGTTCC TCCCCTCCAC AAGACCCCCC TGTGTTGAGG ACCTGCACCG CCAAGCGAAG 
CTCAACCTCA AATCAGTGCT GAGGGAATGT GACAAGCTGA GGCGCGATGG CTGCCGAAGT 
TCTCAGTACT ATTCTCAGGG ACCCACCTTT GCAGCCAGCT CCAGCCCAGG CGATGACTAT 
CAAGAGGAAG ATGCAGAAAC AGACCGAAAG TATTCTCTCT CTTCATCTGA AGAAGAAAGA 
TTTATTGGTA TCAGGAGACC TAAAACGCCA ACCTCAGGTG ACTTCTCTGA CCTTCATACT 
CAGACAAACT GGACCAAGTC CCTGCCGCTG CCAACACCAG AAGAGAAGAC ACGACAGCAA 
GCCCAGACAG TCCAGGCTGA TGTGGTTCCT ATTAACATAA CTGCGTCAGC CACGGGCCAA 
GAGGATGATG GTAGCACCCA TTCTCTATAC TCCCCAGACC ACTACTCTAC ATTAGGAAGG 
CTTGATAGCT ATCGGTCCAC GGGGCAATGC TTGGAAACCA GGGACTCTGG CTGTCAGACA 
GAAGAAGTGA AAGTCATCCC ACCGTCAGTG AGAAGGATCA GGGCTCACAA GGGGCTGGGC 
ATTGCTGCCC AGATGAGCCA CCTCTCGGGT TCCTCTGGCA ACATGTCTGT GCTGAGCGAC 
TCGGCTGGTA TCGTGTTCCC ATCTCGCCTC AATAACGATA CTGGTTTCCA CAGCCTTCCA 
CGCGCTGGCG CAAGGGCAAG CACGTATTCC CTGGAGGCAA GGATGGGTGC CCTGGGCTCT 
GCTGAAGATA CGGATGATAC TTTTCCCTAC CGAGGGGGTA GCTCCCAGGG GCCTGAAAAC 
TTTGCACATT TAGGGGGTGC CTCGAGTACC GGGATGCTTT CGAGGCCCAA ATCCCAGCAG 
CTGAGGCTCT TGGAGAGCCC AGCGTGTGTG GTTTCACCGC ACGCGGCCTA CTCTACCAGC 
GTCATCCCAA ACGCCACACT GCTGTCCTCT TCGGAGGTTA TTGTCATCCA CACCGCTCAG 
AGTGCAGGAC AGCTGGACAG TAGAGTCCCC GGCTCATCCT CATACTCAAA GATAAAACCC 
AGAGACCATC CCACACCCAG GTGCTCTGCG AGAGATGAGC GCCAGTCCCC ACGACATCAC 
TGGAACGAGG GTCATATCAT TCACTCACAG GCTTTAGCCT CCGCTGCCCC CGGTGCCACC 
GCACTGTTAT CCCTCCGTGA CTCAGAGGTC TCTCTCAATG CCCCAGCTAT CAATAGGGAG 
AACGGGTCCC AAGCCATACT TTATCACTGT AGAAGCAACC TGGCCTTTCC TGCCCACCCT 
CCGGATGTGG ATGGCAAGAG CGAGTGCAGT TCTTCAGGGG ACAGGGGATG TGGCAGTTCT 
GAGTCCTGGG AATACAAAGC CTCCAGCAAT GGGCGGGCTT CCCCACTGAA GCCGCACCTT 
GCGACTCCCG GTTGCTCTAC CCCCACAAGT AACATGAGCA GCTGCAGCTT GGACCAAGCA 
TCCCTCAAGG AGGATGCCAG GTCCCTGTAT TCAGAGGACC ACGATGGTTA CTATATGACC 
GCCCAGGGTG ATTCTAGACA CGAGGCTGGG AGCGCGTACG GTATCAGTGA TGGCTTTGGA 
AACCCCAGGC ACAGCACGGT CAATGTTTTT GACGGAAGGG CTCAGAGAAG CCAAGGGGAT 
CAGGCCACTC AGCAGGATAG AATCCTCTCA AGAAACATCT CTCTGAAGAA AGCAAAAAAA 
CCACCACCGC CACCATCCCG GACAGATTCT CTGCGAAGGA TTCCCAAGAA GAACAACCAG 
ACAAATGGGC AGGTGCTCAA CGAGAGCCTG ATCGCCTCAC TCCAGCATTC TCTGCAGCTA 
AGCCTCCCTG GCCAAGGCAG CGGTTCCCCT TCCCAGAGCC CCTGCAGTGA CTGTGAGGAA 
CCCTGGCTCC CCAGGTCCAG AAGCCAGAGC ATTGTTAGCG AGGGTAGTAG CTTGACCTCC 
ACCACCACTC CCAACGTGTA TTCTCTGTGC GGGCTCACTC CATCTCAGAG CGACACAAGC 
AGCGTCAAGT CGGAATACGC AGACCCCTGG GGCTACTACA TTGACTACAC AACTTTGCAG 
GGAGATCCAG GGAACCCCAC AGGGGGCTGC CCAGCCAGTA GTGAGGCAGC CACTGGAAAT 
GGGCCAGTGC GCCACATCCA GGAGGGGTCG AGAGCCCCAG TGCCCCAAGT GCCGGGCTGC 
TCCGTGAAAC CAAAGATCAC CTCACCGGAG AAGTCACAGA GAGTCACCTC TCCATCCAGT 
GGGTATTCTA GCCAGTCGAA TACACCCACA GCACTCACCC CCGTGCCTGT GTTTTTAAAA 
TCGATGTCAC CAGCAAATGG GAAGGGGAAG GCCAAGCCCA AGGTACCAGA AAGAAAATCG 
TCTCTGATCT CTTCAGTGTC CATCTCCTCA TCGTCCACTT CCCTCTCCTC TAATACCTCT 
ACGGAAGGAA GTGGGACCGT GAAGAAACTG GATTCAGCCC TGGCCTCGGC CCTTGCTCCT 
CCCCCTCCTC CTCTTCCCGC GCTGCCTTCT CCGAGTCTGG CAAACAAGTC CCCCTTCCTT 
CCACCTCCTC CTCCTCTAGC AGATTGCTCC GAGGGCTCTC CCTTACCGCC CTCTCCTGTG 
TTCCCCCCTC CACCACCAGA AGCCCTTGTT CCCTTCTGCT CCCCCACTGA CAGGTGCCTT 
TCTCCTTCCC CCACAGCTTT TAGCCCCCCT CTTCCAAGAT GCTCACCTCC CTTGCCAGCA 
CCTCCACCTT TCTTGCCCCC ATCAGAACCC CCACCTGCTC CGCCTCTGGA CCCCAAATTC 
ATGAAAGAAA ACAGGCCCTT TATCAAAAAC TCTAGCCAGT CCGAATCCTC TAGGGAGGCC 
CTCAGGCGGC CTGCCAACAA GGAGGAGGGC TGCCGACCCT CGATGCCCCT GATCACCACA 
GAAGCTTTGC AGATGGTGCA GTTGAGGCCA GTGAGGAAGA ACTCAGGTGC TGAGACGGTC 
CTGTTTTCTG AACCATCAGC TCAGGAACCA CGAACCCCGA CTGCTCCACA GTATCACTTA 
AAGCCATCTG CTTTCCTCAA ATCCCGAAAT AGCATAAATG AAATGGAGAG TGAAAGCCAG 
GCTGCCTCTG TGACGAGCTC GCTTCCGATG CCTGCCAAGA GCCAGAGTCA GGGTGACCAT 
GACAGTGCAG TCGAGCGTGG CGGCCTTCAG AGCTGCAGCG ATGGAGCTCC AGGCCCGGGT 
CCCAGCCTCA GGACCGCTCT GCTCCCAGAC TCTTCACCCA GCAGGAAGCC ACCCCCCATC 
TCCAAGAAGC CCAAACTGTT CCTGGTGGTA CCACCTCCGC AGAGAGATTT CACAGCGGAG 
CCCACAGAGA ACGGGAGCGA AGCTTTCTCA GGCGTGCCCA GCCCTACCGG GGCAGAGGGG 
GAAGCTGGGC AGAGCCAAGA GGAGAAATCC AGCCCAGCAT CCCAAGCTGG CTCTCATGCT 
ACGGCCCCCA CCTCCGGCAG TACGGTTCTA GAAAGAGGAA CTGCAGGGTC CTTGTCCCCT 
GGCGTTGTGG AAGCTAATGC CCCCATGGTC CAACCTAGTA CATCACCGGG CCCCGCACAG 
GAAGAGGCAG GAGAGAACCG CGTGGATGGT GAGAGAAATG TGAAGAGCTG CGCGTCTCCA 
CGGGGCGGAG AAGCTGGGTT GCCGGAACCC AACACAGCTG GTCCGTCTTC AGACACGGCA 
GACGTTTCTA AAGAAGGAGG GAACGATGAG GTGCTGACCC CTACCAGACC CAGGACAACG 
GAAGACCTGT TTGCAGCCAT TCACAGATCC AAAAGGAAAG TCCTTGGTCG GAAGGACTCC 
GAAGATGATC ACACTCGAAA TCATTCTCCC TCCCCGCCTG TGACACCCAC AGGTGCTGCC 
CCTAGCCTGG CCTCTCCAAA GCAAGTAGGG TCCATCCAGC GAAGCCTAAA AAAGAGCAGT 
ACCAGCAGCG ACAACTTCAA AGCTCTGCTG CTCAAGAAGG GGAGTCGCTC GGACACCAGC 
GCTCGCATGT CCGCGGCGGA GATGCTCAAG TCCACGGACC CTCGATTCCA GAGATCTAGG 
TCGGAGCCTT CGCCAGAGGT CCCGGACAGC CCATCCAGCT TCTCTCCCAA TAAGAACAGG 
AGAGCTCCGG AGGAGTGGGC CAAGAATGAA GGACTGATGC CTCGGAGTCT GTCCTTTTCC 
GGCCCTCGGT ACAGCCGCAG CAGGACGCCA CCCTCTGCGG CCAGCAGCAG GTACAGCATG 
AGGAACCGCA TCCAAAGCAG CCCCATGACA GTCATCTCTG AGGGAGAGGG AGAGCCTGCC 
GAGCCCGCAG ACAACAAAGT ACGCAGGACC CTGGACACTA CGAGGGGATG CTCTCTGGAC 
AGACTGGCGG GACAGGAGAC GGACCGGGGC AGCCCGCTGT GTAGCGAGGA GCCTGCCTCT 
GTGGATGGGA TAGGAAGGGC TAAGGACGAC GGTCCCTCGG AGCAGTGTAG GGGTCCTGAA 
CAGAAGAGTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017443407.1
CDS	287..5158
Translation	MPFHQRSVEPARLRRPEEAAGALAGDPPLFRSLEQVSSHTLVCLLAQLADLSRCAGDIFGELEGQAAALGHRTAELHRRLDALHAAAARLDHRRVKIPVSNLDEESRWTVHYTAPWHQQENVFLPSTRPPCVEDLHRQAKLNLKSVLRECDKLRRDGCRSSQYYSQGPTFAASSSPGDDYQEEDAETDRKYSLSSSEEERFIGIRRPKTPTSGDFSDLHTQTNWTKSLPLPTPEEKTRQQAQTVQADVVPINITASATGQEDDGSTHSLYSPDHYSTLGRLDSYRSTGQCLETRDSGCQTEEVKVIPPSVRRIRAHKGLGIAAQMSHLSGSSGNMSVLSDSAGIVFPSRLNNDTGFHSLPRAGARASTYSLEARMGALGSAEDTDDTFPYRGGSSQGPENFAHLGGASSTGMLSRPKSQQLRLLESPACVVSPHAAYSTSVIPNATLLSSSEVIVIHTAQSAGQLDSRVPGSSSYSKIKPRDHPTPRCSARDERQSPRHHWNEGHIIHSQALASAAPGATALLSLRDSEVSLNAPAINRENGSQAILYHCRSNLAFPAHPPDVDGKSECSSSGDRGCGSSESWEYKASSNGRASPLKPHLATPGCSTPTSNMSSCSLDQASLKEDARSLYSEDHDGYYMTAQGDSRHEAGSAYGISDGFGNPRHSTVNVFDGRAQRSQGDQATQQDRILSRNISLKKAKKPPPPPSRTDSLRRIPKKNNQTNGQVLNESLIASLQHSLQLSLPGQGSGSPSQSPCSDCEEPWLPRSRSQSIVSEGSSLTSTTTPNVYSLCGLTPSQSDTSSVKSEYADPWGYYIDYTTLQGDPGNPTGGCPASSEAATGNGPVRHIQEGSRAPVPQVPGCSVKPKITSPEKSQRVTSPSSGYSSQSNTPTALTPVPVFLKSMSPANGKGKAKPKVPERKSSLISSVSISSSSTSLSSNTSTEGSGTVKKLDSALASALAPPPPPLPALPSPSLANKSPFLPPPPPLADCSEGSPLPPSPVFPPPPPEALVPFCSPTDRCLSPSPTAFSPPLPRCSPPLPAPPPFLPPSEPPPAPPLDPKFMKENRPFIKNSSQSESSREALRRPANKEEGCRPSMPLITTEALQMVQLRPVRKNSGAETVLFSEPSAQEPRTPTAPQYHLKPSAFLKSRNSINEMESESQAASVTSSLPMPAKSQSQGDHDSAVERGGLQSCSDGAPGPGPSLRTALLPDSSPSRKPPPISKKPKLFLVVPPPQRDFTAEPTENGSEAFSGVPSPTGAEGEAGQSQEEKSSPASQAGSHATAPTSGSTVLERGTAGSLSPGVVEANAPMVQPSTSPGPAQEEAGENRVDGERNVKSCASPRGGEAGLPEPNTAGPSSDTADVSKEGGNDEVLTPTRPRTTEDLFAAIHRSKRKVLGRKDSEDDHTRNHSPSPPVTPTGAAPSLASPKQVGSIQRSLKKSSTSSDNFKALLLKKGSRSDTSARMSAAEMLKSTDPRFQRSRSEPSPEVPDSPSSFSPNKNRRAPEEWAKNEGLMPRSLSFSGPRYSRSRTPPSAASSRYSMRNRIQSSPMTVISEGEGEPAEPADNKVRRTLDTTRGCSLDRLAGQETDRGSPLCSEEPASVDGIGRAKDDGPSEQCRGPEQKS

Target ORF information:

RefSeq Version	XM_017587918.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus NHS-like 1 (Nhsl1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017587918.1

ORF Insert Sequence:

ATGCCCTTCC ACCAACGGAG CGTGGAGCCC GCACGGCTGC GCCGGCCCGA GGAGGCGGCG 
GGGGCTCTGG CTGGGGACCC GCCGCTCTTC CGCTCGCTGG AGCAGGTCAG CTCGCACACC 
CTGGTCTGCT TGCTCGCCCA GCTGGCCGAC CTGTCGCGCT GCGCTGGGGA CATTTTCGGG 
GAGCTCGAGG GCCAGGCGGC CGCGCTGGGT CACCGCACCG CCGAGTTGCA CCGTCGCCTG 
GACGCCCTGC ACGCTGCCGC CGCGCGCCTG GACCACCGGC GAGTGAAAAT CCCGGTTTCC 
AACTTAGACG AGGAGAGCCG ATGGACCGTC CACTATACGG CGCCGTGGCA CCAGCAGGAG 
AATGTGTTCC TCCCCTCCAC AAGACCCCCC TGTGTTGAGG ACCTGCACCG CCAAGCGAAG 
CTCAACCTCA AATCAGTGCT GAGGGAATGT GACAAGCTGA GGCGCGATGG CTGCCGAAGT 
TCTCAGTACT ATTCTCAGGG ACCCACCTTT GCAGCCAGCT CCAGCCCAGG CGATGACTAT 
CAAGAGGAAG ATGCAGAAAC AGACCGAAAG TATTCTCTCT CTTCATCTGA AGAAGAAAGA 
TTTATTGGTA TCAGGAGACC TAAAACGCCA ACCTCAGGTG ACTTCTCTGA CCTTCATACT 
CAGACAAACT GGACCAAGTC CCTGCCGCTG CCAACACCAG AAGAGAAGAC ACGACAGCAA 
GCCCAGACAG TCCAGGCTGA TGTGGTTCCT ATTAACATAA CTGCGTCAGC CACGGGCCAA 
GAGGATGATG GTAGCACCCA TTCTCTATAC TCCCCAGACC ACTACTCTAC ATTAGGAAGG 
CTTGATAGCT ATCGGTCCAC GGGGCAATGC TTGGAAACCA GGGACTCTGG CTGTCAGACA 
GAAGAAGTGA AAGTCATCCC ACCGTCAGTG AGAAGGATCA GGGCTCACAA GGGGCTGGGC 
ATTGCTGCCC AGATGAGCCA CCTCTCGGGT TCCTCTGGCA ACATGTCTGT GCTGAGCGAC 
TCGGCTGGTA TCGTGTTCCC ATCTCGCCTC AATAACGATA CTGGTTTCCA CAGCCTTCCA 
CGCGCTGGCG CAAGGGCAAG CACGTATTCC CTGGAGGCAA GGATGGGTGC CCTGGGCTCT 
GCTGAAGATA CGGATGATAC TTTTCCCTAC CGAGGGGGTA GCTCCCAGGG GCCTGAAAAC 
TTTGCACATT TAGGGGGTGC CTCGAGTACC GGGATGCTTT CGAGGCCCAA ATCCCAGCAG 
CTGAGGCTCT TGGAGAGCCC AGCGTGTGTG GTTTCACCGC ACGCGGCCTA CTCTACCAGC 
GTCATCCCAA ACGCCACACT GCTGTCCTCT TCGGAGGTTA TTGTCATCCA CACCGCTCAG 
AGTGCAGGAC AGCTGGACAG TAGAGTCCCC GGCTCATCCT CATACTCAAA GATAAAACCC 
AGAGACCATC CCACACCCAG GTGCTCTGCG AGAGATGAGC GCCAGTCCCC ACGACATCAC 
TGGAACGAGG GTCATATCAT TCACTCACAG GCTTTAGCCT CCGCTGCCCC CGGTGCCACC 
GCACTGTTAT CCCTCCGTGA CTCAGAGGTC TCTCTCAATG CCCCAGCTAT CAATAGGGAG 
AACGGGTCCC AAGCCATACT TTATCACTGT AGAAGCAACC TGGCCTTTCC TGCCCACCCT 
CCGGATGTGG ATGGCAAGAG CGAGTGCAGT TCTTCAGGGG ACAGGGGATG TGGCAGTTCT 
GAGTCCTGGG AATACAAAGC CTCCAGCAAT GGGCGGGCTT CCCCACTGAA GCCGCACCTT 
GCGACTCCCG GTTGCTCTAC CCCCACAAGT AACATGAGCA GCTGCAGCTT GGACCAAGCA 
TCCCTCAAGG AGGATGCCAG GTCCCTGTAT TCAGAGGACC ACGATGGTTA CTATATGACC 
GCCCAGGGTG ATTCTAGACA CGAGGCTGGG AGCGCGTACG GTATCAGTGA TGGCTTTGGA 
AACCCCAGGC ACAGCACGGT CAATGTTTTT GACGGAAGGG CTCAGAGAAG CCAAGGGGAT 
CAGGCCACTC AGCAGGATAG AATCCTCTCA AGAAACATCT CTCTGAAGAA AGCAAAAAAA 
CCACCACCGC CACCATCCCG GACAGATTCT CTGCGAAGGA TTCCCAAGAA GAACAACCAG 
ACAAATGGGC AGGTGCTCAA CGAGAGCCTG ATCGCCTCAC TCCAGCATTC TCTGCAGCTA 
AGCCTCCCTG GCCAAGGCAG CGGTTCCCCT TCCCAGAGCC CCTGCAGTGA CTGTGAGGAA 
CCCTGGCTCC CCAGGTCCAG AAGCCAGAGC ATTGTTAGCG AGGGTAGTAG CTTGACCTCC 
ACCACCACTC CCAACGTGTA TTCTCTGTGC GGGCTCACTC CATCTCAGAG CGACACAAGC 
AGCGTCAAGT CGGAATACGC AGACCCCTGG GGCTACTACA TTGACTACAC AACTTTGCAG 
GGAGATCCAG GGAACCCCAC AGGGGGCTGC CCAGCCAGTA GTGAGGCAGC CACTGGAAAT 
GGGCCAGTGC GCCACATCCA GGAGGGGTCG AGAGCCCCAG TGCCCCAAGT GCCGGGCTGC 
TCCGTGAAAC CAAAGATCAC CTCACCGGAG AAGTCACAGA GAGTCACCTC TCCATCCAGT 
GGGTATTCTA GCCAGTCGAA TACACCCACA GCACTCACCC CCGTGCCTGT GTTTTTAAAA 
TCGATGTCAC CAGCAAATGG GAAGGGGAAG GCCAAGCCCA AGGTACCAGA AAGAAAATCG 
TCTCTGATCT CTTCAGTGTC CATCTCCTCA TCGTCCACTT CCCTCTCCTC TAATACCTCT 
ACGGAAGGAA GTGGGACCGT GAAGAAACTG GATTCAGCCC TGGCCTCGGC CCTTGCTCCT 
CCCCCTCCTC CTCTTCCCGC GCTGCCTTCT CCGAGTCTGG CAAACAAGTC CCCCTTCCTT 
CCACCTCCTC CTCCTCTAGC AGATTGCTCC GAGGGCTCTC CCTTACCGCC CTCTCCTGTG 
TTCCCCCCTC CACCACCAGA AGCCCTTGTT CCCTTCTGCT CCCCCACTGA CAGGTGCCTT 
TCTCCTTCCC CCACAGCTTT TAGCCCCCCT CTTCCAAGAT GCTCACCTCC CTTGCCAGCA 
CCTCCACCTT TCTTGCCCCC ATCAGAACCC CCACCTGCTC CGCCTCTGGA CCCCAAATTC 
ATGAAAGAAA ACAGGCCCTT TATCAAAAAC TCTAGCCAGT CCGAATCCTC TAGGGAGGCC 
CTCAGGCGGC CTGCCAACAA GGAGGAGGGC TGCCGACCCT CGATGCCCCT GATCACCACA 
GAAGCTTTGC AGATGGTGCA GTTGAGGCCA GTGAGGAAGA ACTCAGGTGC TGAGACGGTC 
CTGTTTTCTG AACCATCAGC TCAGGAACCA CGAACCCCGA CTGCTCCACA GTATCACTTA 
AAGCCATCTG CTTTCCTCAA ATCCCGAAAT AGCATAAATG AAATGGAGAG TGAAAGCCAG 
GCTGCCTCTG TGACGAGCTC GCTTCCGATG CCTGCCAAGA GCCAGAGTCA GGGTGACCAT 
GACAGTGCAG TCGAGCGTGG CGGCCTTCAG AGCTGCAGCG ATGGAGCTCC AGGCCCGGGT 
CCCAGCCTCA GGACCGCTCT GCTCCCAGAC TCTTCACCCA GCAGGAAGCC ACCCCCCATC 
TCCAAGAAGC CCAAACTGTT CCTGGTGGTA CCACCTCCGC AGAGAGATTT CACAGCGGAG 
CCCACAGAGA ACGGGAGCGA AGCTTTCTCA GGCGTGCCCA GCCCTACCGG GGCAGAGGGG 
GAAGCTGGGC AGAGCCAAGA GGAGAAATCC AGCCCAGCAT CCCAAGCTGG CTCTCATGCT 
ACGGCCCCCA CCTCCGGCAG TACGGTTCTA GAAAGAGGAA CTGCAGGGTC CTTGTCCCCT 
GGCGTTGTGG AAGCTAATGC CCCCATGGTC CAACCTAGTA CATCACCGGG CCCCGCACAG 
GAAGAGGCAG GAGAGAACCG CGTGGATGGT GAGAGAAATG TGAAGAGCTG CGCGTCTCCA 
CGGGGCGGAG AAGCTGGGTT GCCGGAACCC AACACAGCTG GTCCGTCTTC AGACACGGCA 
GACGTTTCTA AAGAAGGAGG GAACGATGAG GTGCTGACCC CTACCAGACC CAGGACAACG 
GAAGACCTGT TTGCAGCCAT TCACAGATCC AAAAGGAAAG TCCTTGGTCG GAAGGACTCC 
GAAGATGATC ACACTCGAAA TCATTCTCCC TCCCCGCCTG TGACACCCAC AGGTGCTGCC 
CCTAGCCTGG CCTCTCCAAA GCAAGTAGGG TCCATCCAGC GAAGCCTAAA AAAGAGCAGT 
ACCAGCAGCG ACAACTTCAA AGCTCTGCTG CTCAAGAAGG GGAGTCGCTC GGACACCAGC 
GCTCGCATGT CCGCGGCGGA GATGCTCAAG TCCACGGACC CTCGATTCCA GAGATCTAGG 
TCGGAGCCTT CGCCAGAGGT CCCGGACAGC CCATCCAGCT TCTCTCCCAA TAAGAACAGG 
AGAGCTCCGG AGGAGTGGGC CAAGAATGAA GGACTGATGC CTCGGAGTCT GTCCTTTTCC 
GGCCCTCGGT ACAGCCGCAG CAGGACGCCA CCCTCTGCGG CCAGCAGCAG GTACAGCATG 
AGGAACCGCA TCCAAAGCAG CCCCATGACA GTCATCTCTG AGGGAGAGGG AGAGCCTGCC 
GAGCCCGCAG ACAACAAAGT ACGCAGGACC CTGGACACTA CGAGGGGATG CTCTCTGGAC 
AGACTGGCGG GACAGGAGAC GGACCGGGGC AGCCCGCTGT GTAGCGAGGA GCCTGCCTCT 
GTGGATGGGA TAGGAAGGGC TAAGGACGAC GGTCCCTCGG AGCAGTGTAG GGGTCCTGAA 
CAGAAGAGTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Nhsl1 ( XM_017589824.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017445313.1 Rattus norvegicus NHS-like 1 (Nhsl1), transcript variant X3, mRNA.

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CloneID	ORa53615
Clone ID Related Accession (Same CDS sequence)	XM_017587918.1 , XM_017589824.1
Accession Version	XM_017589824.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4872bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	NHS-like protein 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCTTCC ACCAACGGAG CGTGGAGCCC GCACGGCTGC GCCGGCCCGA GGAGGCGGCG 
GGGGCTCTGG CTGGGGACCC GCCGCTCTTC CGCTCGCTGG AGCAGGTCAG CTCGCACACC 
CTGGTCTGCT TGCTCGCCCA GCTGGCCGAC CTGTCGCGCT GCGCTGGGGA CATTTTCGGG 
GAGCTCGAGG GCCAGGCGGC CGCGCTGGGT CACCGCACCG CCGAGTTGCA CCGTCGCCTG 
GACGCCCTGC ACGCTGCCGC CGCGCGCCTG GACCACCGGC GAGTGAAAAT CCCGGTTTCC 
AACTTAGACG AGGAGAGCCG ATGGACCGTC CACTATACGG CGCCGTGGCA CCAGCAGGAG 
AATGTGTTCC TCCCCTCCAC AAGACCCCCC TGTGTTGAGG ACCTGCACCG CCAAGCGAAG 
CTCAACCTCA AATCAGTGCT GAGGGAATGT GACAAGCTGA GGCGCGATGG CTGCCGAAGT 
TCTCAGTACT ATTCTCAGGG ACCCACCTTT GCAGCCAGCT CCAGCCCAGG CGATGACTAT 
CAAGAGGAAG ATGCAGAAAC AGACCGAAAG TATTCTCTCT CTTCATCTGA AGAAGAAAGA 
TTTATTGGTA TCAGGAGACC TAAAACGCCA ACCTCAGGTG ACTTCTCTGA CCTTCATACT 
CAGACAAACT GGACCAAGTC CCTGCCGCTG CCAACACCAG AAGAGAAGAC ACGACAGCAA 
GCCCAGACAG TCCAGGCTGA TGTGGTTCCT ATTAACATAA CTGCGTCAGC CACGGGCCAA 
GAGGATGATG GTAGCACCCA TTCTCTATAC TCCCCAGACC ACTACTCTAC ATTAGGAAGG 
CTTGATAGCT ATCGGTCCAC GGGGCAATGC TTGGAAACCA GGGACTCTGG CTGTCAGACA 
GAAGAAGTGA AAGTCATCCC ACCGTCAGTG AGAAGGATCA GGGCTCACAA GGGGCTGGGC 
ATTGCTGCCC AGATGAGCCA CCTCTCGGGT TCCTCTGGCA ACATGTCTGT GCTGAGCGAC 
TCGGCTGGTA TCGTGTTCCC ATCTCGCCTC AATAACGATA CTGGTTTCCA CAGCCTTCCA 
CGCGCTGGCG CAAGGGCAAG CACGTATTCC CTGGAGGCAA GGATGGGTGC CCTGGGCTCT 
GCTGAAGATA CGGATGATAC TTTTCCCTAC CGAGGGGGTA GCTCCCAGGG GCCTGAAAAC 
TTTGCACATT TAGGGGGTGC CTCGAGTACC GGGATGCTTT CGAGGCCCAA ATCCCAGCAG 
CTGAGGCTCT TGGAGAGCCC AGCGTGTGTG GTTTCACCGC ACGCGGCCTA CTCTACCAGC 
GTCATCCCAA ACGCCACACT GCTGTCCTCT TCGGAGGTTA TTGTCATCCA CACCGCTCAG 
AGTGCAGGAC AGCTGGACAG TAGAGTCCCC GGCTCATCCT CATACTCAAA GATAAAACCC 
AGAGACCATC CCACACCCAG GTGCTCTGCG AGAGATGAGC GCCAGTCCCC ACGACATCAC 
TGGAACGAGG GTCATATCAT TCACTCACAG GCTTTAGCCT CCGCTGCCCC CGGTGCCACC 
GCACTGTTAT CCCTCCGTGA CTCAGAGGTC TCTCTCAATG CCCCAGCTAT CAATAGGGAG 
AACGGGTCCC AAGCCATACT TTATCACTGT AGAAGCAACC TGGCCTTTCC TGCCCACCCT 
CCGGATGTGG ATGGCAAGAG CGAGTGCAGT TCTTCAGGGG ACAGGGGATG TGGCAGTTCT 
GAGTCCTGGG AATACAAAGC CTCCAGCAAT GGGCGGGCTT CCCCACTGAA GCCGCACCTT 
GCGACTCCCG GTTGCTCTAC CCCCACAAGT AACATGAGCA GCTGCAGCTT GGACCAAGCA 
TCCCTCAAGG AGGATGCCAG GTCCCTGTAT TCAGAGGACC ACGATGGTTA CTATATGACC 
GCCCAGGGTG ATTCTAGACA CGAGGCTGGG AGCGCGTACG GTATCAGTGA TGGCTTTGGA 
AACCCCAGGC ACAGCACGGT CAATGTTTTT GACGGAAGGG CTCAGAGAAG CCAAGGGGAT 
CAGGCCACTC AGCAGGATAG AATCCTCTCA AGAAACATCT CTCTGAAGAA AGCAAAAAAA 
CCACCACCGC CACCATCCCG GACAGATTCT CTGCGAAGGA TTCCCAAGAA GAACAACCAG 
ACAAATGGGC AGGTGCTCAA CGAGAGCCTG ATCGCCTCAC TCCAGCATTC TCTGCAGCTA 
AGCCTCCCTG GCCAAGGCAG CGGTTCCCCT TCCCAGAGCC CCTGCAGTGA CTGTGAGGAA 
CCCTGGCTCC CCAGGTCCAG AAGCCAGAGC ATTGTTAGCG AGGGTAGTAG CTTGACCTCC 
ACCACCACTC CCAACGTGTA TTCTCTGTGC GGGCTCACTC CATCTCAGAG CGACACAAGC 
AGCGTCAAGT CGGAATACGC AGACCCCTGG GGCTACTACA TTGACTACAC AACTTTGCAG 
GGAGATCCAG GGAACCCCAC AGGGGGCTGC CCAGCCAGTA GTGAGGCAGC CACTGGAAAT 
GGGCCAGTGC GCCACATCCA GGAGGGGTCG AGAGCCCCAG TGCCCCAAGT GCCGGGCTGC 
TCCGTGAAAC CAAAGATCAC CTCACCGGAG AAGTCACAGA GAGTCACCTC TCCATCCAGT 
GGGTATTCTA GCCAGTCGAA TACACCCACA GCACTCACCC CCGTGCCTGT GTTTTTAAAA 
TCGATGTCAC CAGCAAATGG GAAGGGGAAG GCCAAGCCCA AGGTACCAGA AAGAAAATCG 
TCTCTGATCT CTTCAGTGTC CATCTCCTCA TCGTCCACTT CCCTCTCCTC TAATACCTCT 
ACGGAAGGAA GTGGGACCGT GAAGAAACTG GATTCAGCCC TGGCCTCGGC CCTTGCTCCT 
CCCCCTCCTC CTCTTCCCGC GCTGCCTTCT CCGAGTCTGG CAAACAAGTC CCCCTTCCTT 
CCACCTCCTC CTCCTCTAGC AGATTGCTCC GAGGGCTCTC CCTTACCGCC CTCTCCTGTG 
TTCCCCCCTC CACCACCAGA AGCCCTTGTT CCCTTCTGCT CCCCCACTGA CAGGTGCCTT 
TCTCCTTCCC CCACAGCTTT TAGCCCCCCT CTTCCAAGAT GCTCACCTCC CTTGCCAGCA 
CCTCCACCTT TCTTGCCCCC ATCAGAACCC CCACCTGCTC CGCCTCTGGA CCCCAAATTC 
ATGAAAGAAA ACAGGCCCTT TATCAAAAAC TCTAGCCAGT CCGAATCCTC TAGGGAGGCC 
CTCAGGCGGC CTGCCAACAA GGAGGAGGGC TGCCGACCCT CGATGCCCCT GATCACCACA 
GAAGCTTTGC AGATGGTGCA GTTGAGGCCA GTGAGGAAGA ACTCAGGTGC TGAGACGGTC 
CTGTTTTCTG AACCATCAGC TCAGGAACCA CGAACCCCGA CTGCTCCACA GTATCACTTA 
AAGCCATCTG CTTTCCTCAA ATCCCGAAAT AGCATAAATG AAATGGAGAG TGAAAGCCAG 
GCTGCCTCTG TGACGAGCTC GCTTCCGATG CCTGCCAAGA GCCAGAGTCA GGGTGACCAT 
GACAGTGCAG TCGAGCGTGG CGGCCTTCAG AGCTGCAGCG ATGGAGCTCC AGGCCCGGGT 
CCCAGCCTCA GGACCGCTCT GCTCCCAGAC TCTTCACCCA GCAGGAAGCC ACCCCCCATC 
TCCAAGAAGC CCAAACTGTT CCTGGTGGTA CCACCTCCGC AGAGAGATTT CACAGCGGAG 
CCCACAGAGA ACGGGAGCGA AGCTTTCTCA GGCGTGCCCA GCCCTACCGG GGCAGAGGGG 
GAAGCTGGGC AGAGCCAAGA GGAGAAATCC AGCCCAGCAT CCCAAGCTGG CTCTCATGCT 
ACGGCCCCCA CCTCCGGCAG TACGGTTCTA GAAAGAGGAA CTGCAGGGTC CTTGTCCCCT 
GGCGTTGTGG AAGCTAATGC CCCCATGGTC CAACCTAGTA CATCACCGGG CCCCGCACAG 
GAAGAGGCAG GAGAGAACCG CGTGGATGGT GAGAGAAATG TGAAGAGCTG CGCGTCTCCA 
CGGGGCGGAG AAGCTGGGTT GCCGGAACCC AACACAGCTG GTCCGTCTTC AGACACGGCA 
GACGTTTCTA AAGAAGGAGG GAACGATGAG GTGCTGACCC CTACCAGACC CAGGACAACG 
GAAGACCTGT TTGCAGCCAT TCACAGATCC AAAAGGAAAG TCCTTGGTCG GAAGGACTCC 
GAAGATGATC ACACTCGAAA TCATTCTCCC TCCCCGCCTG TGACACCCAC AGGTGCTGCC 
CCTAGCCTGG CCTCTCCAAA GCAAGTAGGG TCCATCCAGC GAAGCCTAAA AAAGAGCAGT 
ACCAGCAGCG ACAACTTCAA AGCTCTGCTG CTCAAGAAGG GGAGTCGCTC GGACACCAGC 
GCTCGCATGT CCGCGGCGGA GATGCTCAAG TCCACGGACC CTCGATTCCA GAGATCTAGG 
TCGGAGCCTT CGCCAGAGGT CCCGGACAGC CCATCCAGCT TCTCTCCCAA TAAGAACAGG 
AGAGCTCCGG AGGAGTGGGC CAAGAATGAA GGACTGATGC CTCGGAGTCT GTCCTTTTCC 
GGCCCTCGGT ACAGCCGCAG CAGGACGCCA CCCTCTGCGG CCAGCAGCAG GTACAGCATG 
AGGAACCGCA TCCAAAGCAG CCCCATGACA GTCATCTCTG AGGGAGAGGG AGAGCCTGCC 
GAGCCCGCAG ACAACAAAGT ACGCAGGACC CTGGACACTA CGAGGGGATG CTCTCTGGAC 
AGACTGGCGG GACAGGAGAC GGACCGGGGC AGCCCGCTGT GTAGCGAGGA GCCTGCCTCT 
GTGGATGGGA TAGGAAGGGC TAAGGACGAC GGTCCCTCGG AGCAGTGTAG GGGTCCTGAA 
CAGAAGAGTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017445313.1
CDS	287..5158
Translation	MPFHQRSVEPARLRRPEEAAGALAGDPPLFRSLEQVSSHTLVCLLAQLADLSRCAGDIFGELEGQAAALGHRTAELHRRLDALHAAAARLDHRRVKIPVSNLDEESRWTVHYTAPWHQQENVFLPSTRPPCVEDLHRQAKLNLKSVLRECDKLRRDGCRSSQYYSQGPTFAASSSPGDDYQEEDAETDRKYSLSSSEEERFIGIRRPKTPTSGDFSDLHTQTNWTKSLPLPTPEEKTRQQAQTVQADVVPINITASATGQEDDGSTHSLYSPDHYSTLGRLDSYRSTGQCLETRDSGCQTEEVKVIPPSVRRIRAHKGLGIAAQMSHLSGSSGNMSVLSDSAGIVFPSRLNNDTGFHSLPRAGARASTYSLEARMGALGSAEDTDDTFPYRGGSSQGPENFAHLGGASSTGMLSRPKSQQLRLLESPACVVSPHAAYSTSVIPNATLLSSSEVIVIHTAQSAGQLDSRVPGSSSYSKIKPRDHPTPRCSARDERQSPRHHWNEGHIIHSQALASAAPGATALLSLRDSEVSLNAPAINRENGSQAILYHCRSNLAFPAHPPDVDGKSECSSSGDRGCGSSESWEYKASSNGRASPLKPHLATPGCSTPTSNMSSCSLDQASLKEDARSLYSEDHDGYYMTAQGDSRHEAGSAYGISDGFGNPRHSTVNVFDGRAQRSQGDQATQQDRILSRNISLKKAKKPPPPPSRTDSLRRIPKKNNQTNGQVLNESLIASLQHSLQLSLPGQGSGSPSQSPCSDCEEPWLPRSRSQSIVSEGSSLTSTTTPNVYSLCGLTPSQSDTSSVKSEYADPWGYYIDYTTLQGDPGNPTGGCPASSEAATGNGPVRHIQEGSRAPVPQVPGCSVKPKITSPEKSQRVTSPSSGYSSQSNTPTALTPVPVFLKSMSPANGKGKAKPKVPERKSSLISSVSISSSSTSLSSNTSTEGSGTVKKLDSALASALAPPPPPLPALPSPSLANKSPFLPPPPPLADCSEGSPLPPSPVFPPPPPEALVPFCSPTDRCLSPSPTAFSPPLPRCSPPLPAPPPFLPPSEPPPAPPLDPKFMKENRPFIKNSSQSESSREALRRPANKEEGCRPSMPLITTEALQMVQLRPVRKNSGAETVLFSEPSAQEPRTPTAPQYHLKPSAFLKSRNSINEMESESQAASVTSSLPMPAKSQSQGDHDSAVERGGLQSCSDGAPGPGPSLRTALLPDSSPSRKPPPISKKPKLFLVVPPPQRDFTAEPTENGSEAFSGVPSPTGAEGEAGQSQEEKSSPASQAGSHATAPTSGSTVLERGTAGSLSPGVVEANAPMVQPSTSPGPAQEEAGENRVDGERNVKSCASPRGGEAGLPEPNTAGPSSDTADVSKEGGNDEVLTPTRPRTTEDLFAAIHRSKRKVLGRKDSEDDHTRNHSPSPPVTPTGAAPSLASPKQVGSIQRSLKKSSTSSDNFKALLLKKGSRSDTSARMSAAEMLKSTDPRFQRSRSEPSPEVPDSPSSFSPNKNRRAPEEWAKNEGLMPRSLSFSGPRYSRSRTPPSAASSRYSMRNRIQSSPMTVISEGEGEPAEPADNKVRRTLDTTRGCSLDRLAGQETDRGSPLCSEEPASVDGIGRAKDDGPSEQCRGPEQKS

Target ORF information:

RefSeq Version	XM_017589824.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus NHS-like 1 (Nhsl1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017589824.1

ORF Insert Sequence:

ATGCCCTTCC ACCAACGGAG CGTGGAGCCC GCACGGCTGC GCCGGCCCGA GGAGGCGGCG 
GGGGCTCTGG CTGGGGACCC GCCGCTCTTC CGCTCGCTGG AGCAGGTCAG CTCGCACACC 
CTGGTCTGCT TGCTCGCCCA GCTGGCCGAC CTGTCGCGCT GCGCTGGGGA CATTTTCGGG 
GAGCTCGAGG GCCAGGCGGC CGCGCTGGGT CACCGCACCG CCGAGTTGCA CCGTCGCCTG 
GACGCCCTGC ACGCTGCCGC CGCGCGCCTG GACCACCGGC GAGTGAAAAT CCCGGTTTCC 
AACTTAGACG AGGAGAGCCG ATGGACCGTC CACTATACGG CGCCGTGGCA CCAGCAGGAG 
AATGTGTTCC TCCCCTCCAC AAGACCCCCC TGTGTTGAGG ACCTGCACCG CCAAGCGAAG 
CTCAACCTCA AATCAGTGCT GAGGGAATGT GACAAGCTGA GGCGCGATGG CTGCCGAAGT 
TCTCAGTACT ATTCTCAGGG ACCCACCTTT GCAGCCAGCT CCAGCCCAGG CGATGACTAT 
CAAGAGGAAG ATGCAGAAAC AGACCGAAAG TATTCTCTCT CTTCATCTGA AGAAGAAAGA 
TTTATTGGTA TCAGGAGACC TAAAACGCCA ACCTCAGGTG ACTTCTCTGA CCTTCATACT 
CAGACAAACT GGACCAAGTC CCTGCCGCTG CCAACACCAG AAGAGAAGAC ACGACAGCAA 
GCCCAGACAG TCCAGGCTGA TGTGGTTCCT ATTAACATAA CTGCGTCAGC CACGGGCCAA 
GAGGATGATG GTAGCACCCA TTCTCTATAC TCCCCAGACC ACTACTCTAC ATTAGGAAGG 
CTTGATAGCT ATCGGTCCAC GGGGCAATGC TTGGAAACCA GGGACTCTGG CTGTCAGACA 
GAAGAAGTGA AAGTCATCCC ACCGTCAGTG AGAAGGATCA GGGCTCACAA GGGGCTGGGC 
ATTGCTGCCC AGATGAGCCA CCTCTCGGGT TCCTCTGGCA ACATGTCTGT GCTGAGCGAC 
TCGGCTGGTA TCGTGTTCCC ATCTCGCCTC AATAACGATA CTGGTTTCCA CAGCCTTCCA 
CGCGCTGGCG CAAGGGCAAG CACGTATTCC CTGGAGGCAA GGATGGGTGC CCTGGGCTCT 
GCTGAAGATA CGGATGATAC TTTTCCCTAC CGAGGGGGTA GCTCCCAGGG GCCTGAAAAC 
TTTGCACATT TAGGGGGTGC CTCGAGTACC GGGATGCTTT CGAGGCCCAA ATCCCAGCAG 
CTGAGGCTCT TGGAGAGCCC AGCGTGTGTG GTTTCACCGC ACGCGGCCTA CTCTACCAGC 
GTCATCCCAA ACGCCACACT GCTGTCCTCT TCGGAGGTTA TTGTCATCCA CACCGCTCAG 
AGTGCAGGAC AGCTGGACAG TAGAGTCCCC GGCTCATCCT CATACTCAAA GATAAAACCC 
AGAGACCATC CCACACCCAG GTGCTCTGCG AGAGATGAGC GCCAGTCCCC ACGACATCAC 
TGGAACGAGG GTCATATCAT TCACTCACAG GCTTTAGCCT CCGCTGCCCC CGGTGCCACC 
GCACTGTTAT CCCTCCGTGA CTCAGAGGTC TCTCTCAATG CCCCAGCTAT CAATAGGGAG 
AACGGGTCCC AAGCCATACT TTATCACTGT AGAAGCAACC TGGCCTTTCC TGCCCACCCT 
CCGGATGTGG ATGGCAAGAG CGAGTGCAGT TCTTCAGGGG ACAGGGGATG TGGCAGTTCT 
GAGTCCTGGG AATACAAAGC CTCCAGCAAT GGGCGGGCTT CCCCACTGAA GCCGCACCTT 
GCGACTCCCG GTTGCTCTAC CCCCACAAGT AACATGAGCA GCTGCAGCTT GGACCAAGCA 
TCCCTCAAGG AGGATGCCAG GTCCCTGTAT TCAGAGGACC ACGATGGTTA CTATATGACC 
GCCCAGGGTG ATTCTAGACA CGAGGCTGGG AGCGCGTACG GTATCAGTGA TGGCTTTGGA 
AACCCCAGGC ACAGCACGGT CAATGTTTTT GACGGAAGGG CTCAGAGAAG CCAAGGGGAT 
CAGGCCACTC AGCAGGATAG AATCCTCTCA AGAAACATCT CTCTGAAGAA AGCAAAAAAA 
CCACCACCGC CACCATCCCG GACAGATTCT CTGCGAAGGA TTCCCAAGAA GAACAACCAG 
ACAAATGGGC AGGTGCTCAA CGAGAGCCTG ATCGCCTCAC TCCAGCATTC TCTGCAGCTA 
AGCCTCCCTG GCCAAGGCAG CGGTTCCCCT TCCCAGAGCC CCTGCAGTGA CTGTGAGGAA 
CCCTGGCTCC CCAGGTCCAG AAGCCAGAGC ATTGTTAGCG AGGGTAGTAG CTTGACCTCC 
ACCACCACTC CCAACGTGTA TTCTCTGTGC GGGCTCACTC CATCTCAGAG CGACACAAGC 
AGCGTCAAGT CGGAATACGC AGACCCCTGG GGCTACTACA TTGACTACAC AACTTTGCAG 
GGAGATCCAG GGAACCCCAC AGGGGGCTGC CCAGCCAGTA GTGAGGCAGC CACTGGAAAT 
GGGCCAGTGC GCCACATCCA GGAGGGGTCG AGAGCCCCAG TGCCCCAAGT GCCGGGCTGC 
TCCGTGAAAC CAAAGATCAC CTCACCGGAG AAGTCACAGA GAGTCACCTC TCCATCCAGT 
GGGTATTCTA GCCAGTCGAA TACACCCACA GCACTCACCC CCGTGCCTGT GTTTTTAAAA 
TCGATGTCAC CAGCAAATGG GAAGGGGAAG GCCAAGCCCA AGGTACCAGA AAGAAAATCG 
TCTCTGATCT CTTCAGTGTC CATCTCCTCA TCGTCCACTT CCCTCTCCTC TAATACCTCT 
ACGGAAGGAA GTGGGACCGT GAAGAAACTG GATTCAGCCC TGGCCTCGGC CCTTGCTCCT 
CCCCCTCCTC CTCTTCCCGC GCTGCCTTCT CCGAGTCTGG CAAACAAGTC CCCCTTCCTT 
CCACCTCCTC CTCCTCTAGC AGATTGCTCC GAGGGCTCTC CCTTACCGCC CTCTCCTGTG 
TTCCCCCCTC CACCACCAGA AGCCCTTGTT CCCTTCTGCT CCCCCACTGA CAGGTGCCTT 
TCTCCTTCCC CCACAGCTTT TAGCCCCCCT CTTCCAAGAT GCTCACCTCC CTTGCCAGCA 
CCTCCACCTT TCTTGCCCCC ATCAGAACCC CCACCTGCTC CGCCTCTGGA CCCCAAATTC 
ATGAAAGAAA ACAGGCCCTT TATCAAAAAC TCTAGCCAGT CCGAATCCTC TAGGGAGGCC 
CTCAGGCGGC CTGCCAACAA GGAGGAGGGC TGCCGACCCT CGATGCCCCT GATCACCACA 
GAAGCTTTGC AGATGGTGCA GTTGAGGCCA GTGAGGAAGA ACTCAGGTGC TGAGACGGTC 
CTGTTTTCTG AACCATCAGC TCAGGAACCA CGAACCCCGA CTGCTCCACA GTATCACTTA 
AAGCCATCTG CTTTCCTCAA ATCCCGAAAT AGCATAAATG AAATGGAGAG TGAAAGCCAG 
GCTGCCTCTG TGACGAGCTC GCTTCCGATG CCTGCCAAGA GCCAGAGTCA GGGTGACCAT 
GACAGTGCAG TCGAGCGTGG CGGCCTTCAG AGCTGCAGCG ATGGAGCTCC AGGCCCGGGT 
CCCAGCCTCA GGACCGCTCT GCTCCCAGAC TCTTCACCCA GCAGGAAGCC ACCCCCCATC 
TCCAAGAAGC CCAAACTGTT CCTGGTGGTA CCACCTCCGC AGAGAGATTT CACAGCGGAG 
CCCACAGAGA ACGGGAGCGA AGCTTTCTCA GGCGTGCCCA GCCCTACCGG GGCAGAGGGG 
GAAGCTGGGC AGAGCCAAGA GGAGAAATCC AGCCCAGCAT CCCAAGCTGG CTCTCATGCT 
ACGGCCCCCA CCTCCGGCAG TACGGTTCTA GAAAGAGGAA CTGCAGGGTC CTTGTCCCCT 
GGCGTTGTGG AAGCTAATGC CCCCATGGTC CAACCTAGTA CATCACCGGG CCCCGCACAG 
GAAGAGGCAG GAGAGAACCG CGTGGATGGT GAGAGAAATG TGAAGAGCTG CGCGTCTCCA 
CGGGGCGGAG AAGCTGGGTT GCCGGAACCC AACACAGCTG GTCCGTCTTC AGACACGGCA 
GACGTTTCTA AAGAAGGAGG GAACGATGAG GTGCTGACCC CTACCAGACC CAGGACAACG 
GAAGACCTGT TTGCAGCCAT TCACAGATCC AAAAGGAAAG TCCTTGGTCG GAAGGACTCC 
GAAGATGATC ACACTCGAAA TCATTCTCCC TCCCCGCCTG TGACACCCAC AGGTGCTGCC 
CCTAGCCTGG CCTCTCCAAA GCAAGTAGGG TCCATCCAGC GAAGCCTAAA AAAGAGCAGT 
ACCAGCAGCG ACAACTTCAA AGCTCTGCTG CTCAAGAAGG GGAGTCGCTC GGACACCAGC 
GCTCGCATGT CCGCGGCGGA GATGCTCAAG TCCACGGACC CTCGATTCCA GAGATCTAGG 
TCGGAGCCTT CGCCAGAGGT CCCGGACAGC CCATCCAGCT TCTCTCCCAA TAAGAACAGG 
AGAGCTCCGG AGGAGTGGGC CAAGAATGAA GGACTGATGC CTCGGAGTCT GTCCTTTTCC 
GGCCCTCGGT ACAGCCGCAG CAGGACGCCA CCCTCTGCGG CCAGCAGCAG GTACAGCATG 
AGGAACCGCA TCCAAAGCAG CCCCATGACA GTCATCTCTG AGGGAGAGGG AGAGCCTGCC 
GAGCCCGCAG ACAACAAAGT ACGCAGGACC CTGGACACTA CGAGGGGATG CTCTCTGGAC 
AGACTGGCGG GACAGGAGAC GGACCGGGGC AGCCCGCTGT GTAGCGAGGA GCCTGCCTCT 
GTGGATGGGA TAGGAAGGGC TAAGGACGAC GGTCCCTCGG AGCAGTGTAG GGGTCCTGAA 
CAGAAGAGTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	Nhsl1
Entrez Gene ID	308631
Full Name	NHS-like 1
Synonyms	RGD1308124
General protein information	Preferred Names NHS-like 1 Names NHS-like protein 1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 1 Map Location: 1p12

Pan troglodytes (chimpanzee)	NHSL1	XP_001171693.2
Mus musculus (house mouse)	Nhsl1	NP_775566.3
Danio rerio (zebrafish)	nhsl1b	XP_005160821.1
Canis lupus familiaris (dog)	NHSL1	XP_005615510.1
Rattus norvegicus (Norway rat)	Nhsl1	XP_006227729.1
	NHSL1	XP_001095123.2
Xenopus tropicalis (tropical clawed frog)	nhsl1	NP_001135597.1
Homo sapiens (human)	NHSL1	NP_065197.1
Bos taurus (cattle)	NHSL1	NP_001178467.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

Nhsl1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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