Grwd1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Grwd1
Entrez Gene ID	308592
Full Name	glutamate-rich WD repeat containing 1
General protein information	Preferred Names glutamate-rich WD repeat containing 1 Names glutamate-rich WD repeat-containing protein 1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 1 Map Location: 1q22

mRNA and Protein(s)

mRNA	Protein	Name
NM_001012067.1	NP_001012067.1	glutamate-rich WD repeat-containing protein 1

Pathways from BioSystems

Homologies

	GRWD1	XP_001113650.1
Bos taurus (cattle)	GRWD1	NP_001069707.1
Kluyveromyces lactis	KLLA0F17237g	XP_455855.1
Arabidopsis thaliana (thale cress)	AT2G19540	NP_179544.1
Danio rerio (zebrafish)	grwd1	NP_001003509.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007125	XP_308636.2
Eremothecium gossypii	AGOS_ADR242C	NP_984338.2
Oryza sativa (rice)	Os11g0134500	NP_001065677.1
Xenopus tropicalis (tropical clawed frog)	grwd1	NP_989268.3
Pan troglodytes (chimpanzee)	GRWD1	XP_003316533.1
Canis lupus familiaris (dog)	GRWD1	XP_854878.1
Mus musculus (house mouse)	Grwd1	NP_700468.2
Drosophila melanogaster (fruit fly)	l(2)09851	NP_001260713.1
Neurospora crassa	NCU09521	XP_958516.1
Rattus norvegicus (Norway rat)	Grwd1	NP_001012067.1
Caenorhabditis elegans (roundworm)	CELE_Y54H5A.1	NP_498091.1
Magnaporthe oryzae (rice blast fungus)	MGG_14350	XP_003717750.1
Homo sapiens (human)	GRWD1	NP_113673.3
Saccharomyces cerevisiae (baker's yeast)	RRB1	NP_013850.1
Schizosaccharomyces pombe (fission yeast)	SPBC1711.07	NP_595880.1

Gene Ontology
Bibliography

Related articles in PubMed

Nucleosome assembly and disassembly activity of GRWD1, a novel Cdt1-binding protein that promotes pre-replication complex formation.
Aizawa M, Sugimoto N, Watanabe S, Yoshida K, Fujita M
Biochimica et biophysica acta1863(11)2739-2748(2016 11)

Cdt1-binding protein GRWD1 is a novel histone-binding protein that facilitates MCM loading through its influence on chromatin architecture.
Sugimoto N, Maehara K, Yoshida K, Yasukouchi S, Osano S, Watanabe S, Aizawa M, Yugawa T, Kiyono T, Kurumizaka H, Ohkawa Y, Fujita M
Nucleic acids research43(12)5898-911(2015 Jul)

A newly uncovered group of distantly related lysine methyltransferases preferentially interact with molecular chaperones to regulate their activity.
Cloutier P, Lavall?e-Adam M, Faubert D, Blanchette M, Coulombe B
PLoS genetics9(1)e1003210(2013)

Insights into RNA biology from an atlas of mammalian mRNA-binding proteins.
Castello A, Fischer B, Eichelbaum K, Horos R, Beckmann BM, Strein C, Davey NE, Humphreys DT, Preiss T, Steinmetz LM, Krijgsveld J, Hentze MW
Cell149(6)1393-406(2012 Jun)

The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts.
Baltz AG, Munschauer M, Schwanh?usser B, Vasile A, Murakawa Y, Schueler M, Youngs N, Penfold-Brown D, Drew K, Milek M, Wyler E, Bonneau R, Selbach M, Dieterich C, Landthaler M
Molecular cell46(5)674-90(2012 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Grwd1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Grwd1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa02148	NM_001012067.1 Latest version!	Rattus norvegicus glutamate-rich WD repeat containing 1 (Grwd1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Grwd1 ( NM_001012067.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001012067.1 Rattus norvegicus glutamate-rich WD repeat containing 1 (Grwd1), mRNA.

Price & Availability↑

CloneID	ORa02148
Clone ID Related Accession (Same CDS sequence)	NM_001012067.1
Accession Version	NM_001012067.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1338bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-16
Organism	Rattus norvegicus(Norway rat)
Product	glutamate-rich WD repeat-containing protein 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC083883.1. On Feb 11, 2005 this sequence version replaced XM_218563.2. ##Evidence-Data-START## Transcript exon combination :: BC083883.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGGCGGCGC GCAAGGGCCG GAGGCACACA TGTGAAACCG GGGAGCCCAT GGAGGCTGAA 
ACCTACGATC TAGGATCCGA AGGCCCATCC CAGGTCTACC TGCCCGGCAG AGGGCCGCCA 
CTAGGAGAAG GGGAGGAGCT GGTAATGGAT GAAGAGGCCT ACGTGCTATA CCATCGTGCG 
CAAACTGGCG CCCCTTGTCT CAGCTTTGAC ATAGTCCGAG ATCACCTGGG CGACAACCGG 
ACAGAGCTCC CTCTCAGTCT TTACCTGTGT GCGGGAACCC AGGCGGAGAG CGCCCAGAGC 
AATAGACTGA TGATGCTTCG GATGCACAAT CTCCATGGGA CCAGGCCGCC ACCCTCAGAG 
GGCAGTGATG ATGAGGATGA TGAAGATGAA GAGGATGAAG AGGAGCGGAA ACCTCAACTG 
GAGCTGGCCA TGGTTCCCCA CTATGGTGGC ATCAACCGAG TTCGGGTATC TTGGCTGGGA 
GAGGAGCCCG TGGCTGGAGT ATGGTCAGAG AAGGGCCAGG TGGAGGTGTT TGCACTGCGA 
CGGCTCCTGC AGGTGGTGGA TGACCCGCAG GCCCTGGCCA TCTTCCTCCG CGATGAGCAG 
GCCCGAGTAA AGCCCATCTT CTCCTTTGCT GGCCACATGG GTGAGGGCTT TGCTCTTGAC 
TGGTCCCCTC GGGTACCCGG CCGCTTGTTG ACTGGTGACT GTCAGAAGAA CATCCATCTC 
TGGACACCCA CGGATGGTGG TTCCTGGAAT GTGGACCAGA GGCCATTTGT GGGCCATACA 
CGCTCTGTGG AGGATCTGCA GTGGTCGCCA ACAGAGGACA CGGTGTTTGC CTCGTGCTCT 
GCGGATGCCT CCATTCGCAT CTGGGACATC CGAGCAGCCC CAGGCAAGGC CTGCATGCTC 
ACCACAGCCG CTGCCCATGA TGGGGATGTC AACGTCATCA GTTGGAGCCG CAGGGAACCC 
TTCCTGCTCA GTGGTGGGGA TGATGGGACC CTCAAAGTCT GGGACCTGCG ACAGTTCAAG 
TCTGGCTCCC CTGTGGCCAC CTTCAAGCAA CACGTGGCCC CCGTGACCTC CGTTGAATGG 
CACCCTCAGG ACAGTGGGGT CTTTGCAGCC TCAGGTGCTG ACAACCAGAT CACACAGTGG 
GACCTGGCAG TTGAGCGGGA CCCAGAGTCA GGTGAGACAG AGACAGACCC TGGGCTGGCG 
GCACTGCCCC AGCAGTTGCT GTTCGTGCAT CAAGGTGAGA CTGACCTGAA GGAGCTACAC 
TGGCACCCAC AGTGCCCTGG GGTCCTGATC AGCACCGCCC TGTCAGGCTT CACCGTCTTC 
AGGACCATCA GTGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001012067.1
CDS	56..1393
Translation	MAARKGRRHTCETGEPMEAETYDLGSEGPSQVYLPGRGPPLGEGEELVMDEEAYVLYHRAQTGAPCLSFDIVRDHLGDNRTELPLSLYLCAGTQAESAQSNRLMMLRMHNLHGTRPPPSEGSDDEDDEDEEDEEERKPQLELAMVPHYGGINRVRVSWLGEEPVAGVWSEKGQVEVFALRRLLQVVDDPQALAIFLRDEQARVKPIFSFAGHMGEGFALDWSPRVPGRLLTGDCQKNIHLWTPTDGGSWNVDQRPFVGHTRSVEDLQWSPTEDTVFASCSADASIRIWDIRAAPGKACMLTTAAAHDGDVNVISWSRREPFLLSGGDDGTLKVWDLRQFKSGSPVATFKQHVAPVTSVEWHPQDSGVFAASGADNQITQWDLAVERDPESGETETDPGLAALPQQLLFVHQGETDLKELHWHPQCPGVLISTALSGFTVFRTISV

Target ORF information:

RefSeq Version	NM_001012067.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus glutamate-rich WD repeat containing 1 (Grwd1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001012067.1

ORF Insert Sequence:

ATGGCGGCGC GCAAGGGCCG GAGGCACACA TGTGAAACCG GGGAGCCCAT GGAGGCTGAA 
ACCTACGATC TAGGATCCGA AGGCCCATCC CAGGTCTACC TGCCCGGCAG AGGGCCGCCA 
CTAGGAGAAG GGGAGGAGCT GGTAATGGAT GAAGAGGCCT ACGTGCTATA CCATCGTGCG 
CAAACTGGCG CCCCTTGTCT CAGCTTTGAC ATAGTCCGAG ATCACCTGGG CGACAACCGG 
ACAGAGCTCC CTCTCAGTCT TTACCTGTGT GCGGGAACCC AGGCGGAGAG CGCCCAGAGC 
AATAGACTGA TGATGCTTCG GATGCACAAT CTCCATGGGA CCAGGCCGCC ACCCTCAGAG 
GGCAGTGATG ATGAGGATGA TGAAGATGAA GAGGATGAAG AGGAGCGGAA ACCTCAACTG 
GAGCTGGCCA TGGTTCCCCA CTATGGTGGC ATCAACCGAG TTCGGGTATC TTGGCTGGGA 
GAGGAGCCCG TGGCTGGAGT ATGGTCAGAG AAGGGCCAGG TGGAGGTGTT TGCACTGCGA 
CGGCTCCTGC AGGTGGTGGA TGACCCGCAG GCCCTGGCCA TCTTCCTCCG CGATGAGCAG 
GCCCGAGTAA AGCCCATCTT CTCCTTTGCT GGCCACATGG GTGAGGGCTT TGCTCTTGAC 
TGGTCCCCTC GGGTACCCGG CCGCTTGTTG ACTGGTGACT GTCAGAAGAA CATCCATCTC 
TGGACACCCA CGGATGGTGG TTCCTGGAAT GTGGACCAGA GGCCATTTGT GGGCCATACA 
CGCTCTGTGG AGGATCTGCA GTGGTCGCCA ACAGAGGACA CGGTGTTTGC CTCGTGCTCT 
GCGGATGCCT CCATTCGCAT CTGGGACATC CGAGCAGCCC CAGGCAAGGC CTGCATGCTC 
ACCACAGCCG CTGCCCATGA TGGGGATGTC AACGTCATCA GTTGGAGCCG CAGGGAACCC 
TTCCTGCTCA GTGGTGGGGA TGATGGGACC CTCAAAGTCT GGGACCTGCG ACAGTTCAAG 
TCTGGCTCCC CTGTGGCCAC CTTCAAGCAA CACGTGGCCC CCGTGACCTC CGTTGAATGG 
CACCCTCAGG ACAGTGGGGT CTTTGCAGCC TCAGGTGCTG ACAACCAGAT CACACAGTGG 
GACCTGGCAG TTGAGCGGGA CCCAGAGTCA GGTGAGACAG AGACAGACCC TGGGCTGGCG 
GCACTGCCCC AGCAGTTGCT GTTCGTGCAT CAAGGTGAGA CTGACCTGAA GGAGCTACAC 
TGGCACCCAC AGTGCCCTGG GGTCCTGATC AGCACCGCCC TGTCAGGCTT CACCGTCTTC 
AGGACCATCA GTGTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Nucleosome assembly and disassembly activity of GRWD1, a novel Cdt1-binding protein that promotes pre-replication complex formation.
Biochimica et biophysica acta1863(11)2739-2748(2016 11)
Aizawa M,Sugimoto N,Watanabe S,Yoshida K,Fujita M

Cdt1-binding protein GRWD1 is a novel histone-binding protein that facilitates MCM loading through its influence on chromatin architecture.
Nucleic acids research43(12)5898-911(2015 Jul)
Sugimoto N,Maehara K,Yoshida K,Yasukouchi S,Osano S,Watanabe S,Aizawa M,Yugawa T,Kiyono T,Kurumizaka H,Ohkawa Y,Fujita M

A newly uncovered group of distantly related lysine methyltransferases preferentially interact with molecular chaperones to regulate their activity.
PLoS genetics9(1)e1003210(2013)
Cloutier P,Lavall?e-Adam M,Faubert D,Blanchette M,Coulombe B

Insights into RNA biology from an atlas of mammalian mRNA-binding proteins.
Cell149(6)1393-406(2012 Jun)
Castello A,Fischer B,Eichelbaum K,Horos R,Beckmann BM,Strein C,Davey NE,Humphreys DT,Preiss T,Steinmetz LM,Krijgsveld J,Hentze MW

The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts.
Molecular cell46(5)674-90(2012 Jun)
Baltz AG,Munschauer M,Schwanh?usser B,Vasile A,Murakawa Y,Schueler M,Youngs N,Penfold-Brown D,Drew K,Milek M,Wyler E,Bonneau R,Selbach M,Dieterich C,Landthaler M

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Grwd1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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