Ap2a1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ap2a1
Entrez Gene ID	308578
Full Name	adaptor-related protein complex 2, alpha 1 subunit
General protein information	Preferred Names adaptor-related protein complex 2, alpha 1 subunit Names AP-2 complex subunit alpha-1 adaptor protein complex AP-2, alpha 1 subunit alpha-adaptin A
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 1 Map Location: 1q22

mRNA and Protein(s)

mRNA	Protein	Name
XM_006229065.3	XP_006229127.1	AP-2 complex subunit alpha-1 isoform X2
XM_006229064.3	XP_006229126.1	AP-2 complex subunit alpha-1 isoform X1
NM_001107511.2	NP_001100981.1	AP-2 complex subunit alpha-1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	AP2A1	XP_003953618.1
Xenopus tropicalis (tropical clawed frog)	ap2a1	NP_001090846.1
Homo sapiens (human)	AP2A1	NP_570603.2
Mus musculus (house mouse)	Ap2a1	NP_031484.1
Canis lupus familiaris (dog)	AP2A1	XP_003638863.1
Rattus norvegicus (Norway rat)	Ap2a1	NP_001100981.1
Danio rerio (zebrafish)	ap2a1	XP_005164119.1
	AP2A1	XP_001115452.1
Bos taurus (cattle)	AP2A1	NP_001178369.1

Gene Ontology
Bibliography

Related articles in PubMed

Surfactant Protein A Enhances Constitutive Immune Functions of Clathrin Heavy Chain and Clathrin Adaptor Protein 2.
Moulakakis C, Steinh?user C, Biedziak D, Freundt K, Reiling N, Stamme C
American journal of respiratory cell and molecular biology55(1)92-104(2016 Jul)

Central role for PICALM in amyloid-? blood-brain barrier transcytosis and clearance.
Zhao Z, Sagare AP, Ma Q, Halliday MR, Kong P, Kisler K, Winkler EA, Ramanathan A, Kanekiyo T, Bu G, Owens NC, Rege SV, Si G, Ahuja A, Zhu D, Miller CA, Schneider JA, Maeda M, Maeda T, Sugawara T, Ichida JK, Zlokovic BV
Nature neuroscience18(7)978-87(2015 Jul)

Antagonistic functions of Dishevelleds regulate Frizzled3 endocytosis via filopodia tips in Wnt-mediated growth cone guidance.
Onishi K, Shafer B, Lo C, Tissir F, Goffinet AM, Zou Y
The Journal of neuroscience : the official journal of the Society for Neuroscience33(49)19071-85(2013 Dec)

Otoferlin couples to clathrin-mediated endocytosis in mature cochlear inner hair cells.
Duncker SV, Franz C, Kuhn S, Schulte U, Campanelli D, Brandt N, Hirt B, Fakler B, Blin N, Ruth P, Engel J, Marcotti W, Zimmermann U, Knipper M
The Journal of neuroscience : the official journal of the Society for Neuroscience33(22)9508-19(2013 May)

Murine homologue of the human KIAA1199 is implicated in hyaluronan binding and depolymerization.
Yoshida H, Nagaoka A, Nakamura S, Sugiyama Y, Okada Y, Inoue S
FEBS open bio3352-6(2013)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

SP-A transiently enhances the basal protein expression of CHC and alpha-adaptin.
Title: Surfactant Protein A Enhances Constitutive Immune Functions of Clathrin Heavy Chain and Clathrin Adaptor Protein 2.

The following Ap2a1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ap2a1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa05356	NM_001107511.2 Latest version!	Rattus norvegicus adaptor related protein complex 2 subunit alpha 1 (Ap2a1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$573.30 $819.00
ORa22727	XM_006229064.3 Latest version!	Rattus norvegicus adaptor-related protein complex 2, alpha 1 subunit (Ap2a1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
ORa22728	XM_006229065.3 Latest version!	Rattus norvegicus adaptor-related protein complex 2, alpha 1 subunit (Ap2a1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ap2a1 ( NM_001107511.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001100981.1 Rattus norvegicus adaptor related protein complex 2 subunit alpha 1 (Ap2a1), mRNA.

Price & Availability↑

CloneID	ORa05356
Clone ID Related Accession (Same CDS sequence)	NM_001107511.2
Accession Version	NM_001107511.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2934bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-09
Organism	Rattus norvegicus(Norway rat)
Product	AP-2 complex subunit alpha-1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CH473979.2. On Mar 3, 2011 this sequence version replaced NM_001107511.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGCCGGCTG TATCCAAAGG CGATGGCATG CGTGGGCTTG CCGTGTTCAT CTCCGACATC 
CGGAACTGCA AGAGCAAAGA GGCTGAGATC AAGAGGATCA ACAAGGAACT GGCCAACATC 
CGCTCCAAGT TCAAAGGGGA CAAGGCCTTG GATGGCTACA GTAAAAAGAA GTATGTGTGT 
AAGCTGCTCT TCATCTTCCT GCTTGGCCAT GACATTGACT TTGGACACAT GGAGGCTGTG 
AACCTGCTAA GCTCCAACAA GTACACAGAG AAGCAGATAG GGTACCTGTT CATCTCAGTC 
CTGGTGAACT CGAACTCAGA GCTGATCCGG CTCATTAACA ACGCTATCAA GAACGACCTG 
GCCAGTCGCA ACCCCACCTT CATGTGCCTG GCTTTGCACT GTATTGCCAA CGTGGGCAGC 
CGTGAGATGG GCGAGGCCTT TGCTGCCGAC ATTCCCCGAA TCCTGGTGGC CGGAGACAGC 
ATGGATAGTG TGAAGCAGAG TGCAGCCCTA TGCCTACTGC GTCTCTACAA GGCCTCCCCC 
GACTTGGTGC CCATGGGCGA GTGGACGGCC CGGGTGGTGC ATTTGCTCAA TGATCAGCAC 
ATGGGAGTGG TCACAGCCGC TGTCAGTCTC ATCACCTGCC TGTGCAAGAA GAATCCAGAT 
GACTTCAAGA CCTGTGTCTC CCTGGCTGTG TCTCGCCTAA GCCGGATTGT CTCCTCAGCC 
TCCACTGACC TCCAGGACTA CACTTACTAC TTCGTTCCTG CACCCTGGCT CTCTGTGAAG 
TTACTGCGGT TGCTACAGTG TTACCCACCG CCAGAGGATG CGGCTGTGAA AGGGCGGTTA 
GTGGAGTGTC TTGAGACTGT GCTCAACAAG GCCCAGGAGC CTCCCAAGTC CAAGAAGGTG 
CAGCACTCCA ATGCCAAGAA CGCCATCCTG TTTGAGACTA TTAGCCTCAT CATCCACTAT 
GACAGTGAGC CCAACCTCCT GGTCCGTGCC TGCAACCAGC TGGGCCAGTT CCTGCAGCAC 
CGAGAGACGA ACCTGCGCTA CCTGGCTCTG GAGAGCATGT GCACCCTGGC CAGCTCCGAG 
TTCTCCCACG AGGCTGTCAA GACCCACATT GATACTGTTA TTAATGCCCT CAAGACTGAG 
CGGGACGTGA GTGTGAGGCA GCGGGCAGCT GATCTGCTGT ATGCCATGTG TGACCGGAGC 
AATGCCAAGC AGATTGTATC AGAGATGCTG CGGTACCTAG AGACGGCTGA CTATGCCATC 
CGAGAGGAGA TTGTGCTGAA GGTGGCCATC TTGGCTGAGA AGTATGCAGT GGACTACAGC 
TGGTATGTGG ACACCATCCT CAACCTCATC CGCATTGCAG GCGACTATGT GAGCGAGGAG 
GTGTGGTACC GAGTGCTGCA GATCGTCACC AACCGTGATG ATGTCCAGGG TTATGCTGCC 
AAGACAGTGT TTGAGGCCCT CCAGGCCCCA GCCTGTCATG AGAACATGGT GAAGGTCGGT 
GGCTACATCC TTGGGGAGTT TGGGAACTTG ATTGCTGGGG ACCCACGCTC CAGCCCACCT 
GTGCAGTTCT CACTGCTGCA CTCCAAGTTC CACCTGTGCA GTGTGGCCAC TCGTGCCTTG 
CTGCTGTCCA CCTACATCAA GTTCATCAAC CTCTTCCCTG AGACCAAGGC CACCATCCAA 
GGTGTTCTTC GTGCCGGCTC CCAGCTGCGC AATGCTGACG TGGAGCTACA GCAGCGGGCA 
GTGGAGTACC TCACCCTCAG CTCTGTAGCT AGCACCGATG TTTTGGCTAC TGTGCTAGAA 
GAAATGCCCC CATTTCCTGA GCGGGAGTCA TCCATTTTGG CTAAGCTGAA GCGTAAGAAG 
GGCCCTGGGG CAGCCAGTGC CCTGGATGAC AGCCGCAGGG ACACCAGCAG CAATGACATC 
AATGGGGGTG TGGAGCCCAC CCCCAGCAAT GTGTCAACCC CCTCACCCTC CGCGGACCTC 
TTGGGGCTGC GGGCAGCCCC TCCCCCTGCT GCACCCCCAG CTCCTGTAGG CGGGAACCTC 
CTGGTGGATG TTTTCTCTGA CGGCCCCACT GCACAGCCCA GCCTGGGTCC CACCCCTGAG 
GAGGCCTTCC TCAGCGAGCT GGAGCCCCCT GCCCCTGAGA GCCCCATGAC TTTGTTGGCT 
GACCCAGCTC CAGCTGCTGA CCCAGGTCCT GAGGACATAG GTCCTCCCAT TCCAGAAGCA 
GATGAACTGC TGAATAAGTT CGTGTGTAAG AATAGTGGGG TCTTGTTTGA GAACCAGCTG 
CTGCAGATCG GAGTCAAGTC TGAGTTCCGG CAGAACCTGG GCCGCATGTA TCTCTTCTAT 
GGCAACAAGA CTTCTGTGCA GTTCCAGAGC TTCTTGCCCA CCGTGGTCCA CCCTGGGGAC 
CTCCAGACTC ATCTGGCGGT GCAGACCAAG CGTGTAGCTG CACAAGTGGA CGGTGGCGCA 
CAGGTGCAGC AAGTACTCAA CATTGAGTGT CTGCGAGACT TCTTGACGCC ACCACTGCTG 
TCGGTGCGCT TCCGGTACGG TGGCACCGCC CAGTCCCTCA CTCTGAAGCT CCCAGTGACC 
ATCAACAAAT TCTTCCAGCC CACAGAGATG GCCGCCCAGG ACTTTTTCCA GCGCTGGAAG 
CAGCTGAGCC TCCCCCTGCA GGAGGCACAG AAAATCTTCA AAGCCAACCA CCCCATGGAT 
GCTGAAGTCA CAAAGGCCAA GCTTCTGGGG TTTGGCTCTG CTCTTCTGGA CAATGTGGAT 
CCCAACCCTG AGAACTTTGT GGGTGCTGGA ATCATCCAGA CGAAAGCCCT GCAGGTGGGG 
TGTCTGCTTC GACTGGAGCC CAATGCCCAG GCCCAAATGT ACCGCCTAAC CCTGCGCACC 
AGCAAAGAGC CTGTGTCCCG TCACTTATGT GAGCTGCTGG CCCAGCAGTT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001100981.1
CDS	1..2934
Translation	MPAVSKGDGMRGLAVFISDIRNCKSKEAEIKRINKELANIRSKFKGDKALDGYSKKKYVCKLLFIFLLGHDIDFGHMEAVNLLSSNKYTEKQIGYLFISVLVNSNSELIRLINNAIKNDLASRNPTFMCLALHCIANVGSREMGEAFAADIPRILVAGDSMDSVKQSAALCLLRLYKASPDLVPMGEWTARVVHLLNDQHMGVVTAAVSLITCLCKKNPDDFKTCVSLAVSRLSRIVSSASTDLQDYTYYFVPAPWLSVKLLRLLQCYPPPEDAAVKGRLVECLETVLNKAQEPPKSKKVQHSNAKNAILFETISLIIHYDSEPNLLVRACNQLGQFLQHRETNLRYLALESMCTLASSEFSHEAVKTHIDTVINALKTERDVSVRQRAADLLYAMCDRSNAKQIVSEMLRYLETADYAIREEIVLKVAILAEKYAVDYSWYVDTILNLIRIAGDYVSEEVWYRVLQIVTNRDDVQGYAAKTVFEALQAPACHENMVKVGGYILGEFGNLIAGDPRSSPPVQFSLLHSKFHLCSVATRALLLSTYIKFINLFPETKATIQGVLRAGSQLRNADVELQQRAVEYLTLSSVASTDVLATVLEEMPPFPERESSILAKLKRKKGPGAASALDDSRRDTSSNDINGGVEPTPSNVSTPSPSADLLGLRAAPPPAAPPAPVGGNLLVDVFSDGPTAQPSLGPTPEEAFLSELEPPAPESPMTLLADPAPAADPGPEDIGPPIPEADELLNKFVCKNSGVLFENQLLQIGVKSEFRQNLGRMYLFYGNKTSVQFQSFLPTVVHPGDLQTHLAVQTKRVAAQVDGGAQVQQVLNIECLRDFLTPPLLSVRFRYGGTAQSLTLKLPVTINKFFQPTEMAAQDFFQRWKQLSLPLQEAQKIFKANHPMDAEVTKAKLLGFGSALLDNVDPNPENFVGAGIIQTKALQVGCLLRLEPNAQAQMYRLTLRTSKEPVSRHLCELLAQQF

Target ORF information:

RefSeq Version	NM_001107511.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus adaptor related protein complex 2 subunit alpha 1 (Ap2a1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001107511.2

ORF Insert Sequence:

ATGCCGGCTG TATCCAAAGG CGATGGCATG CGTGGGCTTG CCGTGTTCAT CTCCGACATC 
CGGAACTGCA AGAGCAAAGA GGCTGAGATC AAGAGGATCA ACAAGGAACT GGCCAACATC 
CGCTCCAAGT TCAAAGGGGA CAAGGCCTTG GATGGCTACA GTAAAAAGAA GTATGTGTGT 
AAGCTGCTCT TCATCTTCCT GCTTGGCCAT GACATTGACT TTGGACACAT GGAGGCTGTG 
AACCTGCTAA GCTCCAACAA GTACACAGAG AAGCAGATAG GGTACCTGTT CATCTCAGTC 
CTGGTGAACT CGAACTCAGA GCTGATCCGG CTCATTAACA ACGCTATCAA GAACGACCTG 
GCCAGTCGCA ACCCCACCTT CATGTGCCTG GCTTTGCACT GTATTGCCAA CGTGGGCAGC 
CGTGAGATGG GCGAGGCCTT TGCTGCCGAC ATTCCCCGAA TCCTGGTGGC CGGAGACAGC 
ATGGATAGTG TGAAGCAGAG TGCAGCCCTA TGCCTACTGC GTCTCTACAA GGCCTCCCCC 
GACTTGGTGC CCATGGGCGA GTGGACGGCC CGGGTGGTGC ATTTGCTCAA TGATCAGCAC 
ATGGGAGTGG TCACAGCCGC TGTCAGTCTC ATCACCTGCC TGTGCAAGAA GAATCCAGAT 
GACTTCAAGA CCTGTGTCTC CCTGGCTGTG TCTCGCCTAA GCCGGATTGT CTCCTCAGCC 
TCCACTGACC TCCAGGACTA CACTTACTAC TTCGTTCCTG CACCCTGGCT CTCTGTGAAG 
TTACTGCGGT TGCTACAGTG TTACCCACCG CCAGAGGATG CGGCTGTGAA AGGGCGGTTA 
GTGGAGTGTC TTGAGACTGT GCTCAACAAG GCCCAGGAGC CTCCCAAGTC CAAGAAGGTG 
CAGCACTCCA ATGCCAAGAA CGCCATCCTG TTTGAGACTA TTAGCCTCAT CATCCACTAT 
GACAGTGAGC CCAACCTCCT GGTCCGTGCC TGCAACCAGC TGGGCCAGTT CCTGCAGCAC 
CGAGAGACGA ACCTGCGCTA CCTGGCTCTG GAGAGCATGT GCACCCTGGC CAGCTCCGAG 
TTCTCCCACG AGGCTGTCAA GACCCACATT GATACTGTTA TTAATGCCCT CAAGACTGAG 
CGGGACGTGA GTGTGAGGCA GCGGGCAGCT GATCTGCTGT ATGCCATGTG TGACCGGAGC 
AATGCCAAGC AGATTGTATC AGAGATGCTG CGGTACCTAG AGACGGCTGA CTATGCCATC 
CGAGAGGAGA TTGTGCTGAA GGTGGCCATC TTGGCTGAGA AGTATGCAGT GGACTACAGC 
TGGTATGTGG ACACCATCCT CAACCTCATC CGCATTGCAG GCGACTATGT GAGCGAGGAG 
GTGTGGTACC GAGTGCTGCA GATCGTCACC AACCGTGATG ATGTCCAGGG TTATGCTGCC 
AAGACAGTGT TTGAGGCCCT CCAGGCCCCA GCCTGTCATG AGAACATGGT GAAGGTCGGT 
GGCTACATCC TTGGGGAGTT TGGGAACTTG ATTGCTGGGG ACCCACGCTC CAGCCCACCT 
GTGCAGTTCT CACTGCTGCA CTCCAAGTTC CACCTGTGCA GTGTGGCCAC TCGTGCCTTG 
CTGCTGTCCA CCTACATCAA GTTCATCAAC CTCTTCCCTG AGACCAAGGC CACCATCCAA 
GGTGTTCTTC GTGCCGGCTC CCAGCTGCGC AATGCTGACG TGGAGCTACA GCAGCGGGCA 
GTGGAGTACC TCACCCTCAG CTCTGTAGCT AGCACCGATG TTTTGGCTAC TGTGCTAGAA 
GAAATGCCCC CATTTCCTGA GCGGGAGTCA TCCATTTTGG CTAAGCTGAA GCGTAAGAAG 
GGCCCTGGGG CAGCCAGTGC CCTGGATGAC AGCCGCAGGG ACACCAGCAG CAATGACATC 
AATGGGGGTG TGGAGCCCAC CCCCAGCAAT GTGTCAACCC CCTCACCCTC CGCGGACCTC 
TTGGGGCTGC GGGCAGCCCC TCCCCCTGCT GCACCCCCAG CTCCTGTAGG CGGGAACCTC 
CTGGTGGATG TTTTCTCTGA CGGCCCCACT GCACAGCCCA GCCTGGGTCC CACCCCTGAG 
GAGGCCTTCC TCAGCGAGCT GGAGCCCCCT GCCCCTGAGA GCCCCATGAC TTTGTTGGCT 
GACCCAGCTC CAGCTGCTGA CCCAGGTCCT GAGGACATAG GTCCTCCCAT TCCAGAAGCA 
GATGAACTGC TGAATAAGTT CGTGTGTAAG AATAGTGGGG TCTTGTTTGA GAACCAGCTG 
CTGCAGATCG GAGTCAAGTC TGAGTTCCGG CAGAACCTGG GCCGCATGTA TCTCTTCTAT 
GGCAACAAGA CTTCTGTGCA GTTCCAGAGC TTCTTGCCCA CCGTGGTCCA CCCTGGGGAC 
CTCCAGACTC ATCTGGCGGT GCAGACCAAG CGTGTAGCTG CACAAGTGGA CGGTGGCGCA 
CAGGTGCAGC AAGTACTCAA CATTGAGTGT CTGCGAGACT TCTTGACGCC ACCACTGCTG 
TCGGTGCGCT TCCGGTACGG TGGCACCGCC CAGTCCCTCA CTCTGAAGCT CCCAGTGACC 
ATCAACAAAT TCTTCCAGCC CACAGAGATG GCCGCCCAGG ACTTTTTCCA GCGCTGGAAG 
CAGCTGAGCC TCCCCCTGCA GGAGGCACAG AAAATCTTCA AAGCCAACCA CCCCATGGAT 
GCTGAAGTCA CAAAGGCCAA GCTTCTGGGG TTTGGCTCTG CTCTTCTGGA CAATGTGGAT 
CCCAACCCTG AGAACTTTGT GGGTGCTGGA ATCATCCAGA CGAAAGCCCT GCAGGTGGGG 
TGTCTGCTTC GACTGGAGCC CAATGCCCAG GCCCAAATGT ACCGCCTAAC CCTGCGCACC 
AGCAAAGAGC CTGTGTCCCG TCACTTATGT GAGCTGCTGG CCCAGCAGTT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ap2a1 ( XM_006229064.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006229126.1 Rattus norvegicus adaptor-related protein complex 2, alpha 1 subunit (Ap2a1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa22727
Clone ID Related Accession (Same CDS sequence)	XM_006229064.3
Accession Version	XM_006229064.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2934bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	AP-2 complex subunit alpha-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006229064.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGGCTG TATCCAAAGG CGATGGCATG CGTGGGCTTG CCGTGTTCAT CTCCGACATC 
CGGAACTGCA AGAGCAAAGA GGCTGAGATC AAGAGGATCA ACAAGGAACT GGCCAACATC 
CGCTCCAAGT TCAAAGGGGA CAAGGCCTTG GATGGCTACA GTAAAAAGAA GTATGTGTGT 
AAGCTGCTCT TCATCTTCCT GCTTGGCCAT GACATTGACT TTGGACACAT GGAGGCTGTG 
AACCTGCTAA GCTCCAACAA GTACACAGAG AAGCAGATAG GGTACCTGTT CATCTCAGTC 
CTGGTGAACT CGAACTCAGA GCTGATCCGG CTCATTAACA ACGCTATCAA GAACGACCTG 
GCCAGTCGCA ACCCCACCTT CATGTGCCTG GCTTTGCACT GTATTGCCAA CGTGGGCAGC 
CGTGAGATGG GCGAGGCCTT TGCTGCCGAC ATTCCCCGAA TCCTGGTGGC CGGAGACAGC 
ATGGATAGTG TGAAGCAGAG TGCAGCCCTA TGCCTACTGC GTCTCTACAA GGCCTCCCCC 
GACTTGGTGC CCATGGGCGA GTGGACGGCC CGGGTGGTGC ATTTGCTCAA TGATCAGCAC 
ATGGGAGTGG TCACAGCCGC TGTCAGTCTC ATCACCTGCC TGTGCAAGAA GAATCCAGAT 
GACTTCAAGA CCTGTGTCTC CCTGGCTGTG TCTCGCCTAA GCCGGATTGT CTCCTCAGCC 
TCCACTGACC TCCAGGACTA CACTTACTAC TTCGTTCCTG CACCCTGGCT CTCTGTGAAG 
TTACTGCGGT TGCTACAGTG TTACCCACCG CCAGAGGATG CGGCTGTGAA AGGGCGGTTA 
GTGGAGTGTC TTGAGACTGT GCTCAACAAG GCCCAGGAGC CTCCCAAGTC CAAGAAGGTG 
CAGCACTCCA ATGCCAAGAA CGCCATCCTG TTTGAGACTA TTAGCCTCAT CATCCACTAT 
GACAGTGAGC CCAACCTCCT GGTCCGTGCC TGCAACCAGC TGGGCCAGTT CCTGCAGCAC 
CGAGAGACGA ACCTGCGCTA CCTGGCTCTG GAGAGCATGT GCACCCTGGC CAGCTCCGAG 
TTCTCCCACG AGGCTGTCAA GACCCACATT GATACTGTTA TTAATGCCCT CAAGACTGAG 
CGGGACGTGA GTGTGAGGCA GCGGGCAGCT GATCTGCTGT ATGCCATGTG TGACCGGAGC 
AATGCCAAGC AGATTGTATC AGAGATGCTG CGGTACCTAG AGACGGCTGA CTATGCCATC 
CGAGAGGAGA TTGTGCTGAA GGTGGCCATC TTGGCTGAGA AGTATGCAGT GGACTACAGC 
TGGTATGTGG ACACCATCCT CAACCTCATC CGCATTGCAG GCGACTATGT GAGCGAGGAG 
GTGTGGTACC GAGTGCTGCA GATCGTCACC AACCGTGATG ATGTCCAGGG TTATGCTGCC 
AAGACAGTGT TTGAGGCCCT CCAGGCCCCA GCCTGTCATG AGAACATGGT GAAGGTCGGT 
GGCTACATCC TTGGGGAGTT TGGGAACTTG ATTGCTGGGG ACCCACGCTC CAGCCCACCT 
GTGCAGTTCT CACTGCTGCA CTCCAAGTTC CACCTGTGCA GTGTGGCCAC TCGTGCCTTG 
CTGCTGTCCA CCTACATCAA GTTCATCAAC CTCTTCCCTG AGACCAAGGC CACCATCCAA 
GGTGTTCTTC GTGCCGGCTC CCAGCTGCGC AATGCTGACG TGGAGCTACA GCAGCGGGCA 
GTGGAGTACC TCACCCTCAG CTCTGTAGCT AGCACCGATG TTTTGGCTAC TGTGCTAGAA 
GAAATGCCCC CATTTCCTGA GCGGGAGTCA TCCATTTTGG CTAAGCTGAA GCGTAAGAAG 
GGCCCTGGGG CAGCCAGTGC CCTGGATGAC AGCCGCAGGG ACACCAGCAG CAATGACATC 
AATGGGGGTG TGGAGCCCAC CCCCAGCAAT GTGTCAACCC CCTCACCCTC CGCGGACCTC 
TTGGGGCTGC GGGCAGCCCC TCCCCCTGCT GCACCCCCAG CTCCTGTAGG CGGGAACCTC 
CTGGTGGATG TTTTCTCTGA CGGCCCCACT GCACAGCCCA GCCTGGGTCC CACCCCTGAG 
GAGGCCTTCC TCAGCGAGCT GGAGCCCCCT GCCCCTGAGA GCCCCATGAC TTTGTTGGCT 
GACCCAGCTC CAGCTGCTGA CCCAGGTCCT GAGGACATAG GTCCTCCCAT TCCAGAAGCA 
GATGAACTGC TGAATAAGTT CGTGTGTAAG AATAGTGGGG TCTTGTTTGA GAACCAGCTG 
CTGCAGATCG GAGTCAAGTC TGAGTTCCGG CAGAACCTGG GCCGCATGTA TCTCTTCTAT 
GGCAACAAGA CTTCTGTGCA GTTCCAGAGC TTCTTGCCCA CCGTGGTCCA CCCTGGGGAC 
CTCCAGACTC AGCTGGCGGT GCAGACCAAG CGTGTAGCTG CACAAGTGGA CGGTGGCGCA 
CAGGTGCAGC AAGTACTCAA CATTGAGTGT CTGCGAGACT TCTTGACGCC ACCACTGCTG 
TCGGTGCGCT TCCGGTACGG TGGCACCGCC CAGTCCCTCA CTCTGAAGCT CCCAGTGACC 
ATCAACAAAT TCTTCCAGCC CACAGAGATG GCCGCCCAGG ACTTTTTCCA GCGCTGGAAG 
CAGCTGAGCC TCCCCCTGCA GGAGGCACAG AAAATCTTCA AAGCCAACCA CCCCATGGAT 
GCTGAAGTCA CAAAGGCCAA GCTTCTGGGG TTTGGCTCTG CTCTTCTGGA CAATGTGGAT 
CCCAACCCTG AGAACTTTGT GGGTGCTGGA ATCATCCAGA CGAAAGCCCT GCAGGTGGGG 
TGTCTGCTTC GACTGGAGCC CAATGCCCAG GCCCAAATGT ACCGCCTAAC CCTGCGCACC 
AGCAAAGAGC CTGTGTCCCG TCACTTATGT GAGCTGCTGG CCCAGCAGTT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006229126.1
CDS	109..3042
Translation	MPAVSKGDGMRGLAVFISDIRNCKSKEAEIKRINKELANIRSKFKGDKALDGYSKKKYVCKLLFIFLLGHDIDFGHMEAVNLLSSNKYTEKQIGYLFISVLVNSNSELIRLINNAIKNDLASRNPTFMCLALHCIANVGSREMGEAFAADIPRILVAGDSMDSVKQSAALCLLRLYKASPDLVPMGEWTARVVHLLNDQHMGVVTAAVSLITCLCKKNPDDFKTCVSLAVSRLSRIVSSASTDLQDYTYYFVPAPWLSVKLLRLLQCYPPPEDAAVKGRLVECLETVLNKAQEPPKSKKVQHSNAKNAILFETISLIIHYDSEPNLLVRACNQLGQFLQHRETNLRYLALESMCTLASSEFSHEAVKTHIDTVINALKTERDVSVRQRAADLLYAMCDRSNAKQIVSEMLRYLETADYAIREEIVLKVAILAEKYAVDYSWYVDTILNLIRIAGDYVSEEVWYRVLQIVTNRDDVQGYAAKTVFEALQAPACHENMVKVGGYILGEFGNLIAGDPRSSPPVQFSLLHSKFHLCSVATRALLLSTYIKFINLFPETKATIQGVLRAGSQLRNADVELQQRAVEYLTLSSVASTDVLATVLEEMPPFPERESSILAKLKRKKGPGAASALDDSRRDTSSNDINGGVEPTPSNVSTPSPSADLLGLRAAPPPAAPPAPVGGNLLVDVFSDGPTAQPSLGPTPEEAFLSELEPPAPESPMTLLADPAPAADPGPEDIGPPIPEADELLNKFVCKNSGVLFENQLLQIGVKSEFRQNLGRMYLFYGNKTSVQFQSFLPTVVHPGDLQTQLAVQTKRVAAQVDGGAQVQQVLNIECLRDFLTPPLLSVRFRYGGTAQSLTLKLPVTINKFFQPTEMAAQDFFQRWKQLSLPLQEAQKIFKANHPMDAEVTKAKLLGFGSALLDNVDPNPENFVGAGIIQTKALQVGCLLRLEPNAQAQMYRLTLRTSKEPVSRHLCELLAQQF

Target ORF information:

RefSeq Version	XM_006229064.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus adaptor-related protein complex 2, alpha 1 subunit (Ap2a1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006229064.3

ORF Insert Sequence:

ATGCCGGCTG TATCCAAAGG CGATGGCATG CGTGGGCTTG CCGTGTTCAT CTCCGACATC 
CGGAACTGCA AGAGCAAAGA GGCTGAGATC AAGAGGATCA ACAAGGAACT GGCCAACATC 
CGCTCCAAGT TCAAAGGGGA CAAGGCCTTG GATGGCTACA GTAAAAAGAA GTATGTGTGT 
AAGCTGCTCT TCATCTTCCT GCTTGGCCAT GACATTGACT TTGGACACAT GGAGGCTGTG 
AACCTGCTAA GCTCCAACAA GTACACAGAG AAGCAGATAG GGTACCTGTT CATCTCAGTC 
CTGGTGAACT CGAACTCAGA GCTGATCCGG CTCATTAACA ACGCTATCAA GAACGACCTG 
GCCAGTCGCA ACCCCACCTT CATGTGCCTG GCTTTGCACT GTATTGCCAA CGTGGGCAGC 
CGTGAGATGG GCGAGGCCTT TGCTGCCGAC ATTCCCCGAA TCCTGGTGGC CGGAGACAGC 
ATGGATAGTG TGAAGCAGAG TGCAGCCCTA TGCCTACTGC GTCTCTACAA GGCCTCCCCC 
GACTTGGTGC CCATGGGCGA GTGGACGGCC CGGGTGGTGC ATTTGCTCAA TGATCAGCAC 
ATGGGAGTGG TCACAGCCGC TGTCAGTCTC ATCACCTGCC TGTGCAAGAA GAATCCAGAT 
GACTTCAAGA CCTGTGTCTC CCTGGCTGTG TCTCGCCTAA GCCGGATTGT CTCCTCAGCC 
TCCACTGACC TCCAGGACTA CACTTACTAC TTCGTTCCTG CACCCTGGCT CTCTGTGAAG 
TTACTGCGGT TGCTACAGTG TTACCCACCG CCAGAGGATG CGGCTGTGAA AGGGCGGTTA 
GTGGAGTGTC TTGAGACTGT GCTCAACAAG GCCCAGGAGC CTCCCAAGTC CAAGAAGGTG 
CAGCACTCCA ATGCCAAGAA CGCCATCCTG TTTGAGACTA TTAGCCTCAT CATCCACTAT 
GACAGTGAGC CCAACCTCCT GGTCCGTGCC TGCAACCAGC TGGGCCAGTT CCTGCAGCAC 
CGAGAGACGA ACCTGCGCTA CCTGGCTCTG GAGAGCATGT GCACCCTGGC CAGCTCCGAG 
TTCTCCCACG AGGCTGTCAA GACCCACATT GATACTGTTA TTAATGCCCT CAAGACTGAG 
CGGGACGTGA GTGTGAGGCA GCGGGCAGCT GATCTGCTGT ATGCCATGTG TGACCGGAGC 
AATGCCAAGC AGATTGTATC AGAGATGCTG CGGTACCTAG AGACGGCTGA CTATGCCATC 
CGAGAGGAGA TTGTGCTGAA GGTGGCCATC TTGGCTGAGA AGTATGCAGT GGACTACAGC 
TGGTATGTGG ACACCATCCT CAACCTCATC CGCATTGCAG GCGACTATGT GAGCGAGGAG 
GTGTGGTACC GAGTGCTGCA GATCGTCACC AACCGTGATG ATGTCCAGGG TTATGCTGCC 
AAGACAGTGT TTGAGGCCCT CCAGGCCCCA GCCTGTCATG AGAACATGGT GAAGGTCGGT 
GGCTACATCC TTGGGGAGTT TGGGAACTTG ATTGCTGGGG ACCCACGCTC CAGCCCACCT 
GTGCAGTTCT CACTGCTGCA CTCCAAGTTC CACCTGTGCA GTGTGGCCAC TCGTGCCTTG 
CTGCTGTCCA CCTACATCAA GTTCATCAAC CTCTTCCCTG AGACCAAGGC CACCATCCAA 
GGTGTTCTTC GTGCCGGCTC CCAGCTGCGC AATGCTGACG TGGAGCTACA GCAGCGGGCA 
GTGGAGTACC TCACCCTCAG CTCTGTAGCT AGCACCGATG TTTTGGCTAC TGTGCTAGAA 
GAAATGCCCC CATTTCCTGA GCGGGAGTCA TCCATTTTGG CTAAGCTGAA GCGTAAGAAG 
GGCCCTGGGG CAGCCAGTGC CCTGGATGAC AGCCGCAGGG ACACCAGCAG CAATGACATC 
AATGGGGGTG TGGAGCCCAC CCCCAGCAAT GTGTCAACCC CCTCACCCTC CGCGGACCTC 
TTGGGGCTGC GGGCAGCCCC TCCCCCTGCT GCACCCCCAG CTCCTGTAGG CGGGAACCTC 
CTGGTGGATG TTTTCTCTGA CGGCCCCACT GCACAGCCCA GCCTGGGTCC CACCCCTGAG 
GAGGCCTTCC TCAGCGAGCT GGAGCCCCCT GCCCCTGAGA GCCCCATGAC TTTGTTGGCT 
GACCCAGCTC CAGCTGCTGA CCCAGGTCCT GAGGACATAG GTCCTCCCAT TCCAGAAGCA 
GATGAACTGC TGAATAAGTT CGTGTGTAAG AATAGTGGGG TCTTGTTTGA GAACCAGCTG 
CTGCAGATCG GAGTCAAGTC TGAGTTCCGG CAGAACCTGG GCCGCATGTA TCTCTTCTAT 
GGCAACAAGA CTTCTGTGCA GTTCCAGAGC TTCTTGCCCA CCGTGGTCCA CCCTGGGGAC 
CTCCAGACTC AGCTGGCGGT GCAGACCAAG CGTGTAGCTG CACAAGTGGA CGGTGGCGCA 
CAGGTGCAGC AAGTACTCAA CATTGAGTGT CTGCGAGACT TCTTGACGCC ACCACTGCTG 
TCGGTGCGCT TCCGGTACGG TGGCACCGCC CAGTCCCTCA CTCTGAAGCT CCCAGTGACC 
ATCAACAAAT TCTTCCAGCC CACAGAGATG GCCGCCCAGG ACTTTTTCCA GCGCTGGAAG 
CAGCTGAGCC TCCCCCTGCA GGAGGCACAG AAAATCTTCA AAGCCAACCA CCCCATGGAT 
GCTGAAGTCA CAAAGGCCAA GCTTCTGGGG TTTGGCTCTG CTCTTCTGGA CAATGTGGAT 
CCCAACCCTG AGAACTTTGT GGGTGCTGGA ATCATCCAGA CGAAAGCCCT GCAGGTGGGG 
TGTCTGCTTC GACTGGAGCC CAATGCCCAG GCCCAAATGT ACCGCCTAAC CCTGCGCACC 
AGCAAAGAGC CTGTGTCCCG TCACTTATGT GAGCTGCTGG CCCAGCAGTT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ap2a1 ( XM_006229065.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006229127.1 Rattus norvegicus adaptor-related protein complex 2, alpha 1 subunit (Ap2a1), transcript variant X2, mRNA.

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CloneID	ORa22728
Clone ID Related Accession (Same CDS sequence)	XM_006229065.3
Accession Version	XM_006229065.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2868bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	AP-2 complex subunit alpha-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006229065.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGGCTG TATCCAAAGG CGATGGCATG CGTGGGCTTG CCGTGTTCAT CTCCGACATC 
CGGAACTGCA AGAGCAAAGA GGCTGAGATC AAGAGGATCA ACAAGGAACT GGCCAACATC 
CGCTCCAAGT TCAAAGGGGA CAAGGCCTTG GATGGCTACA GTAAAAAGAA GTATGTGTGT 
AAGCTGCTCT TCATCTTCCT GCTTGGCCAT GACATTGACT TTGGACACAT GGAGGCTGTG 
AACCTGCTAA GCTCCAACAA GTACACAGAG AAGCAGATAG GGTACCTGTT CATCTCAGTC 
CTGGTGAACT CGAACTCAGA GCTGATCCGG CTCATTAACA ACGCTATCAA GAACGACCTG 
GCCAGTCGCA ACCCCACCTT CATGTGCCTG GCTTTGCACT GTATTGCCAA CGTGGGCAGC 
CGTGAGATGG GCGAGGCCTT TGCTGCCGAC ATTCCCCGAA TCCTGGTGGC CGGAGACAGC 
ATGGATAGTG TGAAGCAGAG TGCAGCCCTA TGCCTACTGC GTCTCTACAA GGCCTCCCCC 
GACTTGGTGC CCATGGGCGA GTGGACGGCC CGGGTGGTGC ATTTGCTCAA TGATCAGCAC 
ATGGGAGTGG TCACAGCCGC TGTCAGTCTC ATCACCTGCC TGTGCAAGAA GAATCCAGAT 
GACTTCAAGA CCTGTGTCTC CCTGGCTGTG TCTCGCCTAA GCCGGATTGT CTCCTCAGCC 
TCCACTGACC TCCAGGACTA CACTTACTAC TTCGTTCCTG CACCCTGGCT CTCTGTGAAG 
TTACTGCGGT TGCTACAGTG TTACCCACCG CCAGAGGATG CGGCTGTGAA AGGGCGGTTA 
GTGGAGTGTC TTGAGACTGT GCTCAACAAG GCCCAGGAGC CTCCCAAGTC CAAGAAGGTG 
CAGCACTCCA ATGCCAAGAA CGCCATCCTG TTTGAGACTA TTAGCCTCAT CATCCACTAT 
GACAGTGAGC CCAACCTCCT GGTCCGTGCC TGCAACCAGC TGGGCCAGTT CCTGCAGCAC 
CGAGAGACGA ACCTGCGCTA CCTGGCTCTG GAGAGCATGT GCACCCTGGC CAGCTCCGAG 
TTCTCCCACG AGGCTGTCAA GACCCACATT GATACTGTTA TTAATGCCCT CAAGACTGAG 
CGGGACGTGA GTGTGAGGCA GCGGGCAGCT GATCTGCTGT ATGCCATGTG TGACCGGAGC 
AATGCCAAGC AGATTGTATC AGAGATGCTG CGGTACCTAG AGACGGCTGA CTATGCCATC 
CGAGAGGAGA TTGTGCTGAA GGTGGCCATC TTGGCTGAGA AGTATGCAGT GGACTACAGC 
TGGTATGTGG ACACCATCCT CAACCTCATC CGCATTGCAG GCGACTATGT GAGCGAGGAG 
GTGTGGTACC GAGTGCTGCA GATCGTCACC AACCGTGATG ATGTCCAGGG TTATGCTGCC 
AAGACAGTGT TTGAGGCCCT CCAGGCCCCA GCCTGTCATG AGAACATGGT GAAGGTCGGT 
GGCTACATCC TTGGGGAGTT TGGGAACTTG ATTGCTGGGG ACCCACGCTC CAGCCCACCT 
GTGCAGTTCT CACTGCTGCA CTCCAAGTTC CACCTGTGCA GTGTGGCCAC TCGTGCCTTG 
CTGCTGTCCA CCTACATCAA GTTCATCAAC CTCTTCCCTG AGACCAAGGC CACCATCCAA 
GGTGTTCTTC GTGCCGGCTC CCAGCTGCGC AATGCTGACG TGGAGCTACA GCAGCGGGCA 
GTGGAGTACC TCACCCTCAG CTCTGTAGCT AGCACCGATG TTTTGGCTAC TGTGCTAGAA 
GAAATGCCCC CATTTCCTGA GCGGGAGTCA TCCATTTTGG CTAAGCTGAA GCGTAAGAAG 
GGCCCTGGGG CAGCCAGTGC CCTGGATGAC AGCCGCAGGG ACACCAGCAG CAATGACATC 
AATGGGGGTG TGGAGCCCAC CCCCAGCAAT GTGTCAACCC CCTCACCCTC CGCGGACCTC 
TTGGGGCTGC GGGCAGCCCC TCCCCCTGCT GCACCCCCAG CTCCTGTAGG CGGGAACCTC 
CTGGTGGATG TTTTCTCTGA CGGCCCCACT GCACAGCCCA GCCTGGGTCC CACCCCTGAG 
GAGGCCTTCC TCAGCCCAGG TCCTGAGGAC ATAGGTCCTC CCATTCCAGA AGCAGATGAA 
CTGCTGAATA AGTTCGTGTG TAAGAATAGT GGGGTCTTGT TTGAGAACCA GCTGCTGCAG 
ATCGGAGTCA AGTCTGAGTT CCGGCAGAAC CTGGGCCGCA TGTATCTCTT CTATGGCAAC 
AAGACTTCTG TGCAGTTCCA GAGCTTCTTG CCCACCGTGG TCCACCCTGG GGACCTCCAG 
ACTCAGCTGG CGGTGCAGAC CAAGCGTGTA GCTGCACAAG TGGACGGTGG CGCACAGGTG 
CAGCAAGTAC TCAACATTGA GTGTCTGCGA GACTTCTTGA CGCCACCACT GCTGTCGGTG 
CGCTTCCGGT ACGGTGGCAC CGCCCAGTCC CTCACTCTGA AGCTCCCAGT GACCATCAAC 
AAATTCTTCC AGCCCACAGA GATGGCCGCC CAGGACTTTT TCCAGCGCTG GAAGCAGCTG 
AGCCTCCCCC TGCAGGAGGC ACAGAAAATC TTCAAAGCCA ACCACCCCAT GGATGCTGAA 
GTCACAAAGG CCAAGCTTCT GGGGTTTGGC TCTGCTCTTC TGGACAATGT GGATCCCAAC 
CCTGAGAACT TTGTGGGTGC TGGAATCATC CAGACGAAAG CCCTGCAGGT GGGGTGTCTG 
CTTCGACTGG AGCCCAATGC CCAGGCCCAA ATGTACCGCC TAACCCTGCG CACCAGCAAA 
GAGCCTGTGT CCCGTCACTT ATGTGAGCTG CTGGCCCAGC AGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006229127.1
CDS	109..2976
Translation	MPAVSKGDGMRGLAVFISDIRNCKSKEAEIKRINKELANIRSKFKGDKALDGYSKKKYVCKLLFIFLLGHDIDFGHMEAVNLLSSNKYTEKQIGYLFISVLVNSNSELIRLINNAIKNDLASRNPTFMCLALHCIANVGSREMGEAFAADIPRILVAGDSMDSVKQSAALCLLRLYKASPDLVPMGEWTARVVHLLNDQHMGVVTAAVSLITCLCKKNPDDFKTCVSLAVSRLSRIVSSASTDLQDYTYYFVPAPWLSVKLLRLLQCYPPPEDAAVKGRLVECLETVLNKAQEPPKSKKVQHSNAKNAILFETISLIIHYDSEPNLLVRACNQLGQFLQHRETNLRYLALESMCTLASSEFSHEAVKTHIDTVINALKTERDVSVRQRAADLLYAMCDRSNAKQIVSEMLRYLETADYAIREEIVLKVAILAEKYAVDYSWYVDTILNLIRIAGDYVSEEVWYRVLQIVTNRDDVQGYAAKTVFEALQAPACHENMVKVGGYILGEFGNLIAGDPRSSPPVQFSLLHSKFHLCSVATRALLLSTYIKFINLFPETKATIQGVLRAGSQLRNADVELQQRAVEYLTLSSVASTDVLATVLEEMPPFPERESSILAKLKRKKGPGAASALDDSRRDTSSNDINGGVEPTPSNVSTPSPSADLLGLRAAPPPAAPPAPVGGNLLVDVFSDGPTAQPSLGPTPEEAFLSPGPEDIGPPIPEADELLNKFVCKNSGVLFENQLLQIGVKSEFRQNLGRMYLFYGNKTSVQFQSFLPTVVHPGDLQTQLAVQTKRVAAQVDGGAQVQQVLNIECLRDFLTPPLLSVRFRYGGTAQSLTLKLPVTINKFFQPTEMAAQDFFQRWKQLSLPLQEAQKIFKANHPMDAEVTKAKLLGFGSALLDNVDPNPENFVGAGIIQTKALQVGCLLRLEPNAQAQMYRLTLRTSKEPVSRHLCELLAQQF

Target ORF information:

RefSeq Version	XM_006229065.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus adaptor-related protein complex 2, alpha 1 subunit (Ap2a1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006229065.3

ORF Insert Sequence:

ATGCCGGCTG TATCCAAAGG CGATGGCATG CGTGGGCTTG CCGTGTTCAT CTCCGACATC 
CGGAACTGCA AGAGCAAAGA GGCTGAGATC AAGAGGATCA ACAAGGAACT GGCCAACATC 
CGCTCCAAGT TCAAAGGGGA CAAGGCCTTG GATGGCTACA GTAAAAAGAA GTATGTGTGT 
AAGCTGCTCT TCATCTTCCT GCTTGGCCAT GACATTGACT TTGGACACAT GGAGGCTGTG 
AACCTGCTAA GCTCCAACAA GTACACAGAG AAGCAGATAG GGTACCTGTT CATCTCAGTC 
CTGGTGAACT CGAACTCAGA GCTGATCCGG CTCATTAACA ACGCTATCAA GAACGACCTG 
GCCAGTCGCA ACCCCACCTT CATGTGCCTG GCTTTGCACT GTATTGCCAA CGTGGGCAGC 
CGTGAGATGG GCGAGGCCTT TGCTGCCGAC ATTCCCCGAA TCCTGGTGGC CGGAGACAGC 
ATGGATAGTG TGAAGCAGAG TGCAGCCCTA TGCCTACTGC GTCTCTACAA GGCCTCCCCC 
GACTTGGTGC CCATGGGCGA GTGGACGGCC CGGGTGGTGC ATTTGCTCAA TGATCAGCAC 
ATGGGAGTGG TCACAGCCGC TGTCAGTCTC ATCACCTGCC TGTGCAAGAA GAATCCAGAT 
GACTTCAAGA CCTGTGTCTC CCTGGCTGTG TCTCGCCTAA GCCGGATTGT CTCCTCAGCC 
TCCACTGACC TCCAGGACTA CACTTACTAC TTCGTTCCTG CACCCTGGCT CTCTGTGAAG 
TTACTGCGGT TGCTACAGTG TTACCCACCG CCAGAGGATG CGGCTGTGAA AGGGCGGTTA 
GTGGAGTGTC TTGAGACTGT GCTCAACAAG GCCCAGGAGC CTCCCAAGTC CAAGAAGGTG 
CAGCACTCCA ATGCCAAGAA CGCCATCCTG TTTGAGACTA TTAGCCTCAT CATCCACTAT 
GACAGTGAGC CCAACCTCCT GGTCCGTGCC TGCAACCAGC TGGGCCAGTT CCTGCAGCAC 
CGAGAGACGA ACCTGCGCTA CCTGGCTCTG GAGAGCATGT GCACCCTGGC CAGCTCCGAG 
TTCTCCCACG AGGCTGTCAA GACCCACATT GATACTGTTA TTAATGCCCT CAAGACTGAG 
CGGGACGTGA GTGTGAGGCA GCGGGCAGCT GATCTGCTGT ATGCCATGTG TGACCGGAGC 
AATGCCAAGC AGATTGTATC AGAGATGCTG CGGTACCTAG AGACGGCTGA CTATGCCATC 
CGAGAGGAGA TTGTGCTGAA GGTGGCCATC TTGGCTGAGA AGTATGCAGT GGACTACAGC 
TGGTATGTGG ACACCATCCT CAACCTCATC CGCATTGCAG GCGACTATGT GAGCGAGGAG 
GTGTGGTACC GAGTGCTGCA GATCGTCACC AACCGTGATG ATGTCCAGGG TTATGCTGCC 
AAGACAGTGT TTGAGGCCCT CCAGGCCCCA GCCTGTCATG AGAACATGGT GAAGGTCGGT 
GGCTACATCC TTGGGGAGTT TGGGAACTTG ATTGCTGGGG ACCCACGCTC CAGCCCACCT 
GTGCAGTTCT CACTGCTGCA CTCCAAGTTC CACCTGTGCA GTGTGGCCAC TCGTGCCTTG 
CTGCTGTCCA CCTACATCAA GTTCATCAAC CTCTTCCCTG AGACCAAGGC CACCATCCAA 
GGTGTTCTTC GTGCCGGCTC CCAGCTGCGC AATGCTGACG TGGAGCTACA GCAGCGGGCA 
GTGGAGTACC TCACCCTCAG CTCTGTAGCT AGCACCGATG TTTTGGCTAC TGTGCTAGAA 
GAAATGCCCC CATTTCCTGA GCGGGAGTCA TCCATTTTGG CTAAGCTGAA GCGTAAGAAG 
GGCCCTGGGG CAGCCAGTGC CCTGGATGAC AGCCGCAGGG ACACCAGCAG CAATGACATC 
AATGGGGGTG TGGAGCCCAC CCCCAGCAAT GTGTCAACCC CCTCACCCTC CGCGGACCTC 
TTGGGGCTGC GGGCAGCCCC TCCCCCTGCT GCACCCCCAG CTCCTGTAGG CGGGAACCTC 
CTGGTGGATG TTTTCTCTGA CGGCCCCACT GCACAGCCCA GCCTGGGTCC CACCCCTGAG 
GAGGCCTTCC TCAGCCCAGG TCCTGAGGAC ATAGGTCCTC CCATTCCAGA AGCAGATGAA 
CTGCTGAATA AGTTCGTGTG TAAGAATAGT GGGGTCTTGT TTGAGAACCA GCTGCTGCAG 
ATCGGAGTCA AGTCTGAGTT CCGGCAGAAC CTGGGCCGCA TGTATCTCTT CTATGGCAAC 
AAGACTTCTG TGCAGTTCCA GAGCTTCTTG CCCACCGTGG TCCACCCTGG GGACCTCCAG 
ACTCAGCTGG CGGTGCAGAC CAAGCGTGTA GCTGCACAAG TGGACGGTGG CGCACAGGTG 
CAGCAAGTAC TCAACATTGA GTGTCTGCGA GACTTCTTGA CGCCACCACT GCTGTCGGTG 
CGCTTCCGGT ACGGTGGCAC CGCCCAGTCC CTCACTCTGA AGCTCCCAGT GACCATCAAC 
AAATTCTTCC AGCCCACAGA GATGGCCGCC CAGGACTTTT TCCAGCGCTG GAAGCAGCTG 
AGCCTCCCCC TGCAGGAGGC ACAGAAAATC TTCAAAGCCA ACCACCCCAT GGATGCTGAA 
GTCACAAAGG CCAAGCTTCT GGGGTTTGGC TCTGCTCTTC TGGACAATGT GGATCCCAAC 
CCTGAGAACT TTGTGGGTGC TGGAATCATC CAGACGAAAG CCCTGCAGGT GGGGTGTCTG 
CTTCGACTGG AGCCCAATGC CCAGGCCCAA ATGTACCGCC TAACCCTGCG CACCAGCAAA 
GAGCCTGTGT CCCGTCACTT ATGTGAGCTG CTGGCCCAGC AGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Surfactant Protein A Enhances Constitutive Immune Functions of Clathrin Heavy Chain and Clathrin Adaptor Protein 2.
American journal of respiratory cell and molecular biology55(1)92-104(2016 Jul)
Moulakakis C,Steinh?user C,Biedziak D,Freundt K,Reiling N,Stamme C

Central role for PICALM in amyloid-? blood-brain barrier transcytosis and clearance.
Nature neuroscience18(7)978-87(2015 Jul)
Zhao Z,Sagare AP,Ma Q,Halliday MR,Kong P,Kisler K,Winkler EA,Ramanathan A,Kanekiyo T,Bu G,Owens NC,Rege SV,Si G,Ahuja A,Zhu D,Miller CA,Schneider JA,Maeda M,Maeda T,Sugawara T,Ichida JK,Zlokovic BV

Antagonistic functions of Dishevelleds regulate Frizzled3 endocytosis via filopodia tips in Wnt-mediated growth cone guidance.
The Journal of neuroscience : the official journal of the Society for Neuroscience33(49)19071-85(2013 Dec)
Onishi K,Shafer B,Lo C,Tissir F,Goffinet AM,Zou Y

Otoferlin couples to clathrin-mediated endocytosis in mature cochlear inner hair cells.
The Journal of neuroscience : the official journal of the Society for Neuroscience33(22)9508-19(2013 May)
Duncker SV,Franz C,Kuhn S,Schulte U,Campanelli D,Brandt N,Hirt B,Fakler B,Blin N,Ruth P,Engel J,Marcotti W,Zimmermann U,Knipper M

Murine homologue of the human KIAA1199 is implicated in hyaluronan binding and depolymerization.
FEBS open bio3352-6(2013)
Yoshida H,Nagaoka A,Nakamura S,Sugiyama Y,Okada Y,Inoue S

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Ap2a1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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