Egln2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Egln2
Entrez Gene ID	308457
Full Name	egl-9 family hypoxia-inducible factor 2
Synonyms	HIF-PH1,HPH-1,HPH-3,PHD-1,PHD1
General protein information	Preferred Names egl-9 family hypoxia-inducible factor 2 Names egl nine homolog 2 HIF-1alpha prolyl-4-hydroxylase-1 HIF-prolyl hydroxylase 1 hypoxia-inducible factor prolyl hydroxylase 1 prolyl hydroxylase domain-containing protein 1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 1 Map Location: 1q21
Summary	HIF-prolyl hydroxylase 1; might be involved in might play a role in hypoxic preconditioning [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_006228575.3	XP_006228637.1	egl nine homolog 2 isoform X2
NM_001004083.1	NP_001004083.1	egl nine homolog 2
XM_006228574.3	XP_006228636.1	egl nine homolog 2 isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	EGLN2	NP_001233442.1
Xenopus tropicalis (tropical clawed frog)	egln2	XP_002938856.2
Homo sapiens (human)	EGLN2	NP_542770.2
	EGLN2	XP_001099832.1
Mus musculus (house mouse)	Egln2	NP_444438.2
Rattus norvegicus (Norway rat)	Egln2	NP_001004083.1
Canis lupus familiaris (dog)	EGLN2	XP_541609.1

Gene Ontology
Bibliography

Related articles in PubMed

Synergistic effect of HIF-1? and FoxO3a trigger cardiomyocyte apoptosis under hyperglycemic ischemia condition.
Chen YF, Pandey S, Day CH, Chen YF, Jiang AZ, Ho TJ, Chen RJ, Padma VV, Kuo WW, Huang CY
Journal of cellular physiology233(4)3660-3671(2018 Apr)

1,2,4-Triazolo-[1,5-a]pyridine HIF Prolylhydroxylase Domain-1 (PHD-1) Inhibitors With a Novel Monodentate Binding Interaction.
Ahmed S, Ayscough A, Barker GR, Canning HE, Davenport R, Downham R, Harrison D, Jenkins K, Kinsella N, Livermore DG, Wright S, Ivetac AD, Skene R, Wilkens SJ, Webster NA, Hendrick AG
Journal of medicinal chemistry60(13)5663-5672(2017 Jul)

Transmembrane prolyl 4-hydroxylase is a fourth prolyl 4-hydroxylase regulating EPO production and erythropoiesis.
Laitala A, Aro E, Walkinshaw G, M?ki JM, Rossi M, Heikkil? M, Savolainen ER, Arend M, Kivirikko KI, Koivunen P, Myllyharju J
Blood120(16)3336-44(2012 Oct)

Low-level laser therapy alleviates neuropathic pain and promotes function recovery in rats with chronic constriction injury: possible involvements in hypoxia-inducible factor 1? (HIF-1?).
Hsieh YL, Chou LW, Chang PL, Yang CC, Kao MJ, Hong CZ
The Journal of comparative neurology520(13)2903-16(2012 Sep)

Hypoxia inducible factor 1 links fast-patterned muscle activity and fast muscle phenotype in rats.
Lunde IG, Anton SL, Bruusgaard JC, Rana ZA, Ellefsen S, Gundersen K
The Journal of physiology589(Pt 6)1443-54(2011 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

In conclusion, a low-level laser could modulate HIF-1alpha activity in nerve entrapment neuropathy
Title: Low-level laser therapy alleviates neuropathic pain and promotes function recovery in rats with chronic constriction injury: possible involvements in hypoxia-inducible factor 1? (HIF-1?).

HIF-1alpha is overexpressed for 14 days after somatic gene transfer in adult rats, and a slow-to-fast transformation is observed in muscle fibers.
Title: Hypoxia inducible factor 1 links fast-patterned muscle activity and fast muscle phenotype in rats.

Report increased prolyl 4-hydroxylase expression and differential regulation of hypoxia-inducible factors in the aged rat brain.
Title: Increased prolyl 4-hydroxylase expression and differential regulation of hypoxia-inducible factors in the aged rat brain.

Increased mTOR-dependent HIF-1alpha synthesis and decreased hydroxylase-dependent HIF-1alpha degradation contribute to intermittent hypoxia-evoked HIF-1alpha accumulation.
Title: Induction of HIF-1alpha expression by intermittent hypoxia: involvement of NADPH oxidase, Ca2+ signaling, prolyl hydroxylases, and mTOR.

HIF-1alpha may be involved in the protective effect of preceding hypoxia on ischemia/reperfusion injury in the right ventricle
Title: Protection of the right ventricle from ischemia and reperfusion by preceding hypoxia.

The following Egln2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Egln2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa09973	NM_001004083.1 Latest version!	Rattus norvegicus egl-9 family hypoxia-inducible factor 2 (Egln2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00
ORa09973	XM_006228575.3 Latest version!	Rattus norvegicus egl-9 family hypoxia-inducible factor 2 (Egln2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00
ORa22483	XM_006228574.3 Latest version!	Rattus norvegicus egl-9 family hypoxia-inducible factor 2 (Egln2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Egln2 ( NM_001004083.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001004083.1 Rattus norvegicus egl-9 family hypoxia-inducible factor 2 (Egln2), mRNA.

Price & Availability↑

CloneID	ORa09973
Clone ID Related Accession (Same CDS sequence)	XM_006228575.3 , NM_001004083.1
Accession Version	NM_001004083.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1248bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-14
Organism	Rattus norvegicus(Norway rat)
Product	egl nine homolog 2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC078907.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC078907.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGGACAGCC CGTGCCAGCC GCAGGCCCTG AATCAAGCTC TCCCTCAGTT GCCAGGGTCT 
GTGTCAGAGT CCTTGGAGCC TAGCCGAGCC AGGATGGGGG TGGAGAGTTA CCTGCCCTGT 
CCTCTGCTCC CCTCCTACCA CCGTTCAGGA GCATCTGGGG AGGCCTCGGC TGGCAACGGG 
ACCCCCAGAA CCACAGCCAC TGCTACTACT ACCACTGCCA GTCCCCTGCG GGAGGGCTTT 
GGTGGGCAGG ATGGTGGTGA GCTGTGGCCA CTGCAGAGTG AAGGTGCTGC TGCGTTGGTT 
ACCAAGGAGT GCCAGCGACT GGCGGCCCAG GGTGCCCGAC CTGAGGCCCC CAAACGGAAG 
TGGGCCAAGG ATGGTGGGGA TGCCCCTTCA CCCAGCAAGC GACCGTGGGC CAGGCAAGAG 
AACCAGGAGG CCAAGGGGGA AAGTGGTGTG GGCTGTGACA GCGGTGGCGG CAGCAGCAAC 
AGCACTACCC ATAGCAGTGG CGAGGCAAGT TCTAGGCTGA GGGAGGAAGC CCAGCCCTCT 
GCACCTGAGC GCCTGGCCTT GGACTATATT GTGCCTTGCA TGCGGTACTA TGGTATCTGC 
GTCAAGGACA ACTTCTTGGG GGCAGTACTG GGTGGCCGTG TGCTGGCTGA GGTGGAAGCC 
CTGAAGTGGG GCGGGCGTCT ACGTGATGGG CAACTAGTGA GCCAGCGGGC GATCCCACCG 
CGCAGCATTC GTGGGGACCA GATTGCCTGG GTAGAAGGCC ACGAGCCAGG CTGCCGGAGC 
ATTGGTGCCC TCATGGCTCA TGTGGACGCA GTAATCCGCC ACTGTGCAGG GCGGCTGGGC 
AACTACGTCA TCAATGGGCG CACCAAGGCC ATGGTGGCGT GTTACCCAGG CAATGGGCTC 
GGGTACGTGA GGCATGTTGA CAATCCCCAC GGCGATGGGC GCTGCATCAC CTGTATCTAT 
TACCTGAATC AGAACTGGGA TGTTAAGGTG CATGGCGGCC TGCTGCAGAT CTTCCCTGAG 
GGTCGGCCAG TGGTAGCCAA CATCGAGCCA CTCTTTGACC GGTTGCTCAT TTTCTGGTCT 
GACCGACGGA ATCCACATGA GGTGAAGCCA GCCTATGCCA CCAGGTACGC CATCACTGTC 
TGGTATTTTG ATGCCAAGGA GCGGGCAGCA GCCAGAGACA AGTATCAGCT AGCATCGGGA 
CAGAAAGGTG TTCAAGTACC CGTGTCACAG CCAGCTACAC CTACCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001004083.1
CDS	271..1518
Translation	MDSPCQPQALNQALPQLPGSVSESLEPSRARMGVESYLPCPLLPSYHRSGASGEASAGNGTPRTTATATTTTASPLREGFGGQDGGELWPLQSEGAAALVTKECQRLAAQGARPEAPKRKWAKDGGDAPSPSKRPWARQENQEAKGESGVGCDSGGGSSNSTTHSSGEASSRLREEAQPSAPERLALDYIVPCMRYYGICVKDNFLGAVLGGRVLAEVEALKWGGRLRDGQLVSQRAIPPRSIRGDQIAWVEGHEPGCRSIGALMAHVDAVIRHCAGRLGNYVINGRTKAMVACYPGNGLGYVRHVDNPHGDGRCITCIYYLNQNWDVKVHGGLLQIFPEGRPVVANIEPLFDRLLIFWSDRRNPHEVKPAYATRYAITVWYFDAKERAAARDKYQLASGQKGVQVPVSQPATPT

Target ORF information:

RefSeq Version	NM_001004083.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus egl-9 family hypoxia-inducible factor 2 (Egln2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001004083.1

ORF Insert Sequence:

ATGGACAGCC CGTGCCAGCC GCAGGCCCTG AATCAAGCTC TCCCTCAGTT GCCAGGGTCT 
GTGTCAGAGT CCTTGGAGCC TAGCCGAGCC AGGATGGGGG TGGAGAGTTA CCTGCCCTGT 
CCTCTGCTCC CCTCCTACCA CCGTTCAGGA GCATCTGGGG AGGCCTCGGC TGGCAACGGG 
ACCCCCAGAA CCACAGCCAC TGCTACTACT ACCACTGCCA GTCCCCTGCG GGAGGGCTTT 
GGTGGGCAGG ATGGTGGTGA GCTGTGGCCA CTGCAGAGTG AAGGTGCTGC TGCGTTGGTT 
ACCAAGGAGT GCCAGCGACT GGCGGCCCAG GGTGCCCGAC CTGAGGCCCC CAAACGGAAG 
TGGGCCAAGG ATGGTGGGGA TGCCCCTTCA CCCAGCAAGC GACCGTGGGC CAGGCAAGAG 
AACCAGGAGG CCAAGGGGGA AAGTGGTGTG GGCTGTGACA GCGGTGGCGG CAGCAGCAAC 
AGCACTACCC ATAGCAGTGG CGAGGCAAGT TCTAGGCTGA GGGAGGAAGC CCAGCCCTCT 
GCACCTGAGC GCCTGGCCTT GGACTATATT GTGCCTTGCA TGCGGTACTA TGGTATCTGC 
GTCAAGGACA ACTTCTTGGG GGCAGTACTG GGTGGCCGTG TGCTGGCTGA GGTGGAAGCC 
CTGAAGTGGG GCGGGCGTCT ACGTGATGGG CAACTAGTGA GCCAGCGGGC GATCCCACCG 
CGCAGCATTC GTGGGGACCA GATTGCCTGG GTAGAAGGCC ACGAGCCAGG CTGCCGGAGC 
ATTGGTGCCC TCATGGCTCA TGTGGACGCA GTAATCCGCC ACTGTGCAGG GCGGCTGGGC 
AACTACGTCA TCAATGGGCG CACCAAGGCC ATGGTGGCGT GTTACCCAGG CAATGGGCTC 
GGGTACGTGA GGCATGTTGA CAATCCCCAC GGCGATGGGC GCTGCATCAC CTGTATCTAT 
TACCTGAATC AGAACTGGGA TGTTAAGGTG CATGGCGGCC TGCTGCAGAT CTTCCCTGAG 
GGTCGGCCAG TGGTAGCCAA CATCGAGCCA CTCTTTGACC GGTTGCTCAT TTTCTGGTCT 
GACCGACGGA ATCCACATGA GGTGAAGCCA GCCTATGCCA CCAGGTACGC CATCACTGTC 
TGGTATTTTG ATGCCAAGGA GCGGGCAGCA GCCAGAGACA AGTATCAGCT AGCATCGGGA 
CAGAAAGGTG TTCAAGTACC CGTGTCACAG CCAGCTACAC CTACCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Egln2 ( XM_006228575.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006228637.1 Rattus norvegicus egl-9 family hypoxia-inducible factor 2 (Egln2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa09973
Clone ID Related Accession (Same CDS sequence)	XM_006228575.3 , NM_001004083.1
Accession Version	XM_006228575.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1248bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	egl nine homolog 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006228575.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACAGCC CGTGCCAGCC GCAGGCCCTG AATCAAGCTC TCCCTCAGTT GCCAGGGTCT 
GTGTCAGAGT CCTTGGAGCC TAGCCGAGCC AGGATGGGGG TGGAGAGTTA CCTGCCCTGT 
CCTCTGCTCC CCTCCTACCA CCGTTCAGGA GCATCTGGGG AGGCCTCGGC TGGCAACGGG 
ACCCCCAGAA CCACAGCCAC TGCTACTACT ACCACTGCCA GTCCCCTGCG GGAGGGCTTT 
GGTGGGCAGG ATGGTGGTGA GCTGTGGCCA CTGCAGAGTG AAGGTGCTGC TGCGTTGGTT 
ACCAAGGAGT GCCAGCGACT GGCGGCCCAG GGTGCCCGAC CTGAGGCCCC CAAACGGAAG 
TGGGCCAAGG ATGGTGGGGA TGCCCCTTCA CCCAGCAAGC GACCGTGGGC CAGGCAAGAG 
AACCAGGAGG CCAAGGGGGA AAGTGGTGTG GGCTGTGACA GCGGTGGCGG CAGCAGCAAC 
AGCACTACCC ATAGCAGTGG CGAGGCAAGT TCTAGGCTGA GGGAGGAAGC CCAGCCCTCT 
GCACCTGAGC GCCTGGCCTT GGACTATATT GTGCCTTGCA TGCGGTACTA TGGTATCTGC 
GTCAAGGACA ACTTCTTGGG GGCAGTACTG GGTGGCCGTG TGCTGGCTGA GGTGGAAGCC 
CTGAAGTGGG GCGGGCGTCT ACGTGATGGG CAACTAGTGA GCCAGCGGGC GATCCCACCG 
CGCAGCATTC GTGGGGACCA GATTGCCTGG GTAGAAGGCC ACGAGCCAGG CTGCCGGAGC 
ATTGGTGCCC TCATGGCTCA TGTGGACGCA GTAATCCGCC ACTGTGCAGG GCGGCTGGGC 
AACTACGTCA TCAATGGGCG CACCAAGGCC ATGGTGGCGT GTTACCCAGG CAATGGGCTC 
GGGTACGTGA GGCATGTTGA CAATCCCCAC GGCGATGGGC GCTGCATCAC CTGTATCTAT 
TACCTGAATC AGAACTGGGA TGTTAAGGTG CATGGCGGCC TGCTGCAGAT CTTCCCTGAG 
GGTCGGCCAG TGGTAGCCAA CATCGAGCCA CTCTTTGACC GGTTGCTCAT TTTCTGGTCT 
GACCGACGGA ATCCACATGA GGTGAAGCCA GCCTATGCCA CCAGGTACGC CATCACTGTC 
TGGTATTTTG ATGCCAAGGA GCGGGCAGCA GCCAGAGACA AGTATCAGCT AGCATCGGGA 
CAGAAAGGTG TTCAAGTACC CGTGTCACAG CCAGCTACAC CTACCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006228637.1
CDS	323..1570
Translation	MDSPCQPQALNQALPQLPGSVSESLEPSRARMGVESYLPCPLLPSYHRSGASGEASAGNGTPRTTATATTTTASPLREGFGGQDGGELWPLQSEGAAALVTKECQRLAAQGARPEAPKRKWAKDGGDAPSPSKRPWARQENQEAKGESGVGCDSGGGSSNSTTHSSGEASSRLREEAQPSAPERLALDYIVPCMRYYGICVKDNFLGAVLGGRVLAEVEALKWGGRLRDGQLVSQRAIPPRSIRGDQIAWVEGHEPGCRSIGALMAHVDAVIRHCAGRLGNYVINGRTKAMVACYPGNGLGYVRHVDNPHGDGRCITCIYYLNQNWDVKVHGGLLQIFPEGRPVVANIEPLFDRLLIFWSDRRNPHEVKPAYATRYAITVWYFDAKERAAARDKYQLASGQKGVQVPVSQPATPT

Target ORF information:

RefSeq Version	XM_006228575.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus egl-9 family hypoxia-inducible factor 2 (Egln2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006228575.3

ORF Insert Sequence:

ATGGACAGCC CGTGCCAGCC GCAGGCCCTG AATCAAGCTC TCCCTCAGTT GCCAGGGTCT 
GTGTCAGAGT CCTTGGAGCC TAGCCGAGCC AGGATGGGGG TGGAGAGTTA CCTGCCCTGT 
CCTCTGCTCC CCTCCTACCA CCGTTCAGGA GCATCTGGGG AGGCCTCGGC TGGCAACGGG 
ACCCCCAGAA CCACAGCCAC TGCTACTACT ACCACTGCCA GTCCCCTGCG GGAGGGCTTT 
GGTGGGCAGG ATGGTGGTGA GCTGTGGCCA CTGCAGAGTG AAGGTGCTGC TGCGTTGGTT 
ACCAAGGAGT GCCAGCGACT GGCGGCCCAG GGTGCCCGAC CTGAGGCCCC CAAACGGAAG 
TGGGCCAAGG ATGGTGGGGA TGCCCCTTCA CCCAGCAAGC GACCGTGGGC CAGGCAAGAG 
AACCAGGAGG CCAAGGGGGA AAGTGGTGTG GGCTGTGACA GCGGTGGCGG CAGCAGCAAC 
AGCACTACCC ATAGCAGTGG CGAGGCAAGT TCTAGGCTGA GGGAGGAAGC CCAGCCCTCT 
GCACCTGAGC GCCTGGCCTT GGACTATATT GTGCCTTGCA TGCGGTACTA TGGTATCTGC 
GTCAAGGACA ACTTCTTGGG GGCAGTACTG GGTGGCCGTG TGCTGGCTGA GGTGGAAGCC 
CTGAAGTGGG GCGGGCGTCT ACGTGATGGG CAACTAGTGA GCCAGCGGGC GATCCCACCG 
CGCAGCATTC GTGGGGACCA GATTGCCTGG GTAGAAGGCC ACGAGCCAGG CTGCCGGAGC 
ATTGGTGCCC TCATGGCTCA TGTGGACGCA GTAATCCGCC ACTGTGCAGG GCGGCTGGGC 
AACTACGTCA TCAATGGGCG CACCAAGGCC ATGGTGGCGT GTTACCCAGG CAATGGGCTC 
GGGTACGTGA GGCATGTTGA CAATCCCCAC GGCGATGGGC GCTGCATCAC CTGTATCTAT 
TACCTGAATC AGAACTGGGA TGTTAAGGTG CATGGCGGCC TGCTGCAGAT CTTCCCTGAG 
GGTCGGCCAG TGGTAGCCAA CATCGAGCCA CTCTTTGACC GGTTGCTCAT TTTCTGGTCT 
GACCGACGGA ATCCACATGA GGTGAAGCCA GCCTATGCCA CCAGGTACGC CATCACTGTC 
TGGTATTTTG ATGCCAAGGA GCGGGCAGCA GCCAGAGACA AGTATCAGCT AGCATCGGGA 
CAGAAAGGTG TTCAAGTACC CGTGTCACAG CCAGCTACAC CTACCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Egln2 ( XM_006228574.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006228636.1 Rattus norvegicus egl-9 family hypoxia-inducible factor 2 (Egln2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa22483
Clone ID Related Accession (Same CDS sequence)	XM_006228574.3
Accession Version	XM_006228574.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1413bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	egl nine homolog 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006228574.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACAGCC CGTGCCAGCC GCAGGCCCTG AATCAAGCTC TCCCTCAGTT GCCAGGGTCT 
GTGTCAGAGT CCTTGGAGCC TAGCCGAGCC AGGATGGGGG TGGAGAGTTA CCTGCCCTGT 
CCTCTGCTCC CCTCCTACCA CCGTTCAGGA GCATCTGGGG AGGCCTCGGC TGGCAACGGG 
ACCCCCAGAA CCACAGCCAC TGCTACTACT ACCACTGCCA GTCCCCTGCG GGAGGGCTTT 
GGTGGGCAGG ATGGTGGTGA GCTGTGGCCA CTGCAGAGTG AAGGTGCTGC TGCGTTGGTT 
ACCAAGGAGT GCCAGCGACT GGCGGCCCAG GGTGCCCGAC CTGAGGCCCC CAAACGGAAG 
TGGGCCAAGG ATGGTGGGGA TGCCCCTTCA CCCAGCAAGC GACCGTGGGC CAGGCAAGAG 
AACCAGGAGG CCAAGGGGGA AAGTGGTGTG GGCTGTGACA GCGGTGGCGG CAGCAGCAAC 
AGCACTACCC ATAGCAGTGG CGAGGCAAGT TCTAGGCTGA GGGAGGAAGC CCAGCCCTCT 
GCACCTGAGC GCCTGGCCTT GGACTATATT GTGCCTTGCA TGCGGTACTA TGGTATCTGC 
GTCAAGGACA ACTTCTTGGG GGCAGTACTG GGTGGCCGTG TGCTGGCTGA GGTGGAAGCC 
CTGAAGTGGG GCGGGCGTCT ACGTGATGGG CAACTAGTGA GCCAGCGGGC GATCCCACCG 
CGCAGCATTC GTGGGGACCA GATTGCCTGG GTAGAAGGCC ACGAGCCAGG CTGCCGGAGC 
ATTGGTGCCC TCATGGCTCA TGTGGACGCA GTAATCCGCC ACTGTGCAGG GCGGCTGGGC 
AACTACGTCA TCAATGGGCG CACCAAGGCT GGACCCAAAA ATACAGCTGT ACATCGTCGG 
GGAGCTCCAG CTTGCAGAGA AGTACCTGAG CGGCGGGGTC AAGACAGATG GTTGCTGAAT 
GGGCAAAGAA CGAAGGATTG TCATGCTAAC TACTCCAGTT TGCTTACTGT GAACTCCCAC 
CTCTTGTGCA GGGCCATGGT GGCGTGTTAC CCAGGCAATG GGCTCGGGTA CGTGAGGCAT 
GTTGACAATC CCCACGGCGA TGGGCGCTGC ATCACCTGTA TCTATTACCT GAATCAGAAC 
TGGGATGTTA AGGTGCATGG CGGCCTGCTG CAGATCTTCC CTGAGGGTCG GCCAGTGGTA 
GCCAACATCG AGCCACTCTT TGACCGGTTG CTCATTTTCT GGTCTGACCG ACGGAATCCA 
CATGAGGTGA AGCCAGCCTA TGCCACCAGG TACGCCATCA CTGTCTGGTA TTTTGATGCC 
AAGGAGCGGG CAGCAGCCAG AGACAAGTAT CAGCTAGCAT CGGGACAGAA AGGTGTTCAA 
GTACCCGTGT CACAGCCAGC TACACCTACC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006228636.1
CDS	112..1524
Translation	MDSPCQPQALNQALPQLPGSVSESLEPSRARMGVESYLPCPLLPSYHRSGASGEASAGNGTPRTTATATTTTASPLREGFGGQDGGELWPLQSEGAAALVTKECQRLAAQGARPEAPKRKWAKDGGDAPSPSKRPWARQENQEAKGESGVGCDSGGGSSNSTTHSSGEASSRLREEAQPSAPERLALDYIVPCMRYYGICVKDNFLGAVLGGRVLAEVEALKWGGRLRDGQLVSQRAIPPRSIRGDQIAWVEGHEPGCRSIGALMAHVDAVIRHCAGRLGNYVINGRTKAGPKNTAVHRRGAPACREVPERRGQDRWLLNGQRTKDCHANYSSLLTVNSHLLCRAMVACYPGNGLGYVRHVDNPHGDGRCITCIYYLNQNWDVKVHGGLLQIFPEGRPVVANIEPLFDRLLIFWSDRRNPHEVKPAYATRYAITVWYFDAKERAAARDKYQLASGQKGVQVPVSQPATPT

Target ORF information:

RefSeq Version	XM_006228574.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus egl-9 family hypoxia-inducible factor 2 (Egln2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006228574.3

ORF Insert Sequence:

ATGGACAGCC CGTGCCAGCC GCAGGCCCTG AATCAAGCTC TCCCTCAGTT GCCAGGGTCT 
GTGTCAGAGT CCTTGGAGCC TAGCCGAGCC AGGATGGGGG TGGAGAGTTA CCTGCCCTGT 
CCTCTGCTCC CCTCCTACCA CCGTTCAGGA GCATCTGGGG AGGCCTCGGC TGGCAACGGG 
ACCCCCAGAA CCACAGCCAC TGCTACTACT ACCACTGCCA GTCCCCTGCG GGAGGGCTTT 
GGTGGGCAGG ATGGTGGTGA GCTGTGGCCA CTGCAGAGTG AAGGTGCTGC TGCGTTGGTT 
ACCAAGGAGT GCCAGCGACT GGCGGCCCAG GGTGCCCGAC CTGAGGCCCC CAAACGGAAG 
TGGGCCAAGG ATGGTGGGGA TGCCCCTTCA CCCAGCAAGC GACCGTGGGC CAGGCAAGAG 
AACCAGGAGG CCAAGGGGGA AAGTGGTGTG GGCTGTGACA GCGGTGGCGG CAGCAGCAAC 
AGCACTACCC ATAGCAGTGG CGAGGCAAGT TCTAGGCTGA GGGAGGAAGC CCAGCCCTCT 
GCACCTGAGC GCCTGGCCTT GGACTATATT GTGCCTTGCA TGCGGTACTA TGGTATCTGC 
GTCAAGGACA ACTTCTTGGG GGCAGTACTG GGTGGCCGTG TGCTGGCTGA GGTGGAAGCC 
CTGAAGTGGG GCGGGCGTCT ACGTGATGGG CAACTAGTGA GCCAGCGGGC GATCCCACCG 
CGCAGCATTC GTGGGGACCA GATTGCCTGG GTAGAAGGCC ACGAGCCAGG CTGCCGGAGC 
ATTGGTGCCC TCATGGCTCA TGTGGACGCA GTAATCCGCC ACTGTGCAGG GCGGCTGGGC 
AACTACGTCA TCAATGGGCG CACCAAGGCT GGACCCAAAA ATACAGCTGT ACATCGTCGG 
GGAGCTCCAG CTTGCAGAGA AGTACCTGAG CGGCGGGGTC AAGACAGATG GTTGCTGAAT 
GGGCAAAGAA CGAAGGATTG TCATGCTAAC TACTCCAGTT TGCTTACTGT GAACTCCCAC 
CTCTTGTGCA GGGCCATGGT GGCGTGTTAC CCAGGCAATG GGCTCGGGTA CGTGAGGCAT 
GTTGACAATC CCCACGGCGA TGGGCGCTGC ATCACCTGTA TCTATTACCT GAATCAGAAC 
TGGGATGTTA AGGTGCATGG CGGCCTGCTG CAGATCTTCC CTGAGGGTCG GCCAGTGGTA 
GCCAACATCG AGCCACTCTT TGACCGGTTG CTCATTTTCT GGTCTGACCG ACGGAATCCA 
CATGAGGTGA AGCCAGCCTA TGCCACCAGG TACGCCATCA CTGTCTGGTA TTTTGATGCC 
AAGGAGCGGG CAGCAGCCAG AGACAAGTAT CAGCTAGCAT CGGGACAGAA AGGTGTTCAA 
GTACCCGTGT CACAGCCAGC TACACCTACC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Synergistic effect of HIF-1? and FoxO3a trigger cardiomyocyte apoptosis under hyperglycemic ischemia condition.
Journal of cellular physiology233(4)3660-3671(2018 Apr)
Chen YF,Pandey S,Day CH,Chen YF,Jiang AZ,Ho TJ,Chen RJ,Padma VV,Kuo WW,Huang CY

1,2,4-Triazolo-[1,5-a]pyridine HIF Prolylhydroxylase Domain-1 (PHD-1) Inhibitors With a Novel Monodentate Binding Interaction.
Journal of medicinal chemistry60(13)5663-5672(2017 Jul)
Ahmed S,Ayscough A,Barker GR,Canning HE,Davenport R,Downham R,Harrison D,Jenkins K,Kinsella N,Livermore DG,Wright S,Ivetac AD,Skene R,Wilkens SJ,Webster NA,Hendrick AG

Transmembrane prolyl 4-hydroxylase is a fourth prolyl 4-hydroxylase regulating EPO production and erythropoiesis.
Blood120(16)3336-44(2012 Oct)
Laitala A,Aro E,Walkinshaw G,M?ki JM,Rossi M,Heikkil? M,Savolainen ER,Arend M,Kivirikko KI,Koivunen P,Myllyharju J

Low-level laser therapy alleviates neuropathic pain and promotes function recovery in rats with chronic constriction injury: possible involvements in hypoxia-inducible factor 1? (HIF-1?).
The Journal of comparative neurology520(13)2903-16(2012 Sep)
Hsieh YL,Chou LW,Chang PL,Yang CC,Kao MJ,Hong CZ

Hypoxia inducible factor 1 links fast-patterned muscle activity and fast muscle phenotype in rats.
The Journal of physiology589(Pt 6)1443-54(2011 Mar)
Lunde IG,Anton SL,Bruusgaard JC,Rana ZA,Ellefsen S,Gundersen K

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Egln2 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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