Slc12a7 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Slc12a7
Entrez Gene ID	308069
Full Name	solute carrier family 12 member 7
Synonyms	Kcc4
General protein information	Preferred Names solute carrier family 12 member 7 Names solute carrier family 12 member 7 K-Cl cotransporter 4 K-Cl cotransporter KCC4 electroneutral potassium-chloride cotransporter 4 furosemide-sensitive KCl cotransporter 4 solute carrier family 12 (potassium/chloride transporter), member 7 solute carrier family 12 (potassium/chloride transporters), member 7
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 1 Map Location: 1p11

mRNA and Protein(s)

mRNA	Protein	Name
NM_001013144.2	NP_001013162.2	solute carrier family 12 member 7
XM_006227789.3	XP_006227851.1	solute carrier family 12 member 7 isoform X2
XM_017589092.1	XP_017444581.1	solute carrier family 12 member 7 isoform X3
XM_017589091.1	XP_017444580.1	solute carrier family 12 member 7 isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	SLC12A7	NP_006589.2
Gallus gallus (chicken)	SLC12A7	NP_001006371.2
Danio rerio (zebrafish)	slc12a7a	XP_686497.6
Canis lupus familiaris (dog)	SLC12A7	XP_005639745.1
Mus musculus (house mouse)	Slc12a7	NP_035520.1
Rattus norvegicus (Norway rat)	Slc12a7	NP_001013162.2
Pan troglodytes (chimpanzee)	SLC12A7	XP_001175174.2
Bos taurus (cattle)	SLC12A7	XP_002696513.2

Gene Ontology
Bibliography

Related articles in PubMed

ZMPSTE24 defends against influenza and other pathogenic viruses.
Fu B, Wang L, Li S, Dorf ME
The Journal of experimental medicine214(4)919-929(2017 Apr)

The WNK-regulated SPAK/OSR1 kinases directly phosphorylate and inhibit the K+-Cl- co-transporters.
de Los Heros P, Alessi DR, Gourlay R, Campbell DG, Deak M, Macartney TJ, Kahle KT, Zhang J
The Biochemical journal458(3)559-73(2014 Mar)

Molecular evidence for a role for K(+)-Cl(-) cotransporters in the kidney.
Melo Z, Cruz-Rangel S, Bautista R, V?zquez N, Casta?eda-Bueno M, Mount DB, Pasantes-Morales H, Mercado A, Gamba G
American journal of physiology. Renal physiology305(10)F1402-11(2013 Nov)

Glycosylation regulates the function and membrane localization of KCC4.
Weng TY, Chiu WT, Liu HS, Cheng HC, Shen MR, Mount DB, Chou CY
Biochimica et biophysica acta1833(5)1133-46(2013 May)

Cell-type specific distribution of chloride transporters in the rat suprachiasmatic nucleus.
Belenky MA, Sollars PJ, Mount DB, Alper SL, Yarom Y, Pickard GE
Neuroscience165(4)1519-37(2010 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data are consistent with a role for KCC4 in salt reabsorption of the thick ascending loop of Henle's loop and acid secretion of the collecting duct.
Title: Molecular evidence for a role for K(+)-Cl(-) cotransporters in the kidney.

Kinetic models of Na+-K+-2Cl- costransporter (NKCC2) and K+-Cl- cotransporter (KCC4), two of the key cotransporters of the Henle limb, are fashioned with inclusion of terms representing binding and transport of NH4+.
Title: A mathematical model of rat ascending Henle limb. I. Cotransporter function.

KCC4 and H+,K+-ATPase are the main machineries for basal HCl secretion in the apical canalicular membrane of the resting parietal cell. They also may contribute in part to massive acid secretion in the stimulated state.
Title: Functional association between K+-Cl- cotransporter-4 and H+,K+-ATPase in the apical canalicular membrane of gastric parietal cells.

In the rat brain, KCC4 express in the choroid plexus and the suprachiasmatic nucleus of the hypothalamus.
Title: KCC3 and KCC4 expression in rat adult forebrain.

distribution of KCC1, KCC3a, KCC3b, KCC4, and KCC2 protein and mrna in pancreatic alpha-cells and beta-cells; KCC's make a significant contribution to volume homeostasis only in alpha-cells
Title: Expression of K+-Cl- cotransporters in the alpha-cells of rat endocrine pancreas.

The following Slc12a7 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Slc12a7 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa56553	XM_017589091.1 Latest version!	Rattus norvegicus solute carrier family 12 member 7 (Slc12a7), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
ORa56554	XM_017589092.1 Latest version!	Rattus norvegicus solute carrier family 12 member 7 (Slc12a7), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
ORa03529	NM_001013144.2 Latest version!	Rattus norvegicus solute carrier family 12 member 7 (Slc12a7), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
ORa22096	XM_006227789.3 Latest version!	Rattus norvegicus solute carrier family 12 member 7 (Slc12a7), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Slc12a7 ( XM_017589091.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017444580.1 Rattus norvegicus solute carrier family 12 member 7 (Slc12a7), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa56553
Clone ID Related Accession (Same CDS sequence)	XM_017589091.1
Accession Version	XM_017589091.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3363bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	solute carrier family 12 member 7 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCCAGC GCTTCGTGGT GATCCCAGCA CAGAGCGGGA CGGGGGACGC GGGACCCAGG 
GCAGAAGGTG GCAGCGGCGG CAGCCAGCGC CTGTGCTCGG ATCCCGACCC CGACCCCGAC 
CCTGACCCCG ACCCCGATCC CAGCCTGGCG GCCGCTCTGA AAACACAAGA AGTAGATGCG 
CTGCCCATTC TGCGCTACTG CCGCGAGCCC AGCCGCTATG GAGATGGAAA CCCCAGGGAG 
AACAGCCCAT TCATCAATAA TGTGGAGGTG GAAAGAGAGA GCTACTTCGA GGGGAAGAAC 
ATGGCGCTTT TTGAGGAAGA GATGGACAGC AACCCCATGG TATCATCACT GCTGAACAAG 
CTGGCCAACT ATACCAACCT GAGCCAGGGT GTGGTAGAGC ATGAGGAAGA TGAGGACAGC 
CGGAGGCGGG AGATCAAGGC CCCACGCATG GGCACCTTCA TCGGAGTCTA CCTACCGTGC 
CTGCAGAACA TCTTGGGTGT AATCCTTTTC CTGCGCCTGA CCTGGATTGT GGGGGCGGCT 
GGCGTTCTGG AGTCCTTCCT CATCGTGGCC ATGTGCTGCA CCTGTACAAT GCTGACAGCC 
ATCTCCATGA GTGCCATCGC TACCAACGGT GTGGTCCCAG CGGGAGGCTC ATACTACATG 
ATCTCCCGTT CGCTGGGGCC TGAGTTTGGA GGGGCTGTTG GCCTCTGCTT CTACTTGGGC 
ACAACGTTCG CAGGCGCCAT GTACATCCTG GGAACCATCG AGATCTTCCT GACCTACATC 
TCTCCAAGTG CAGCCATCTT CCAGGCAGAG ACAGCAGACG GCGAGGCGGC CGCGCTGCTG 
AACAACATGC GTGTGTATGG CAGCTGCGCA CTGGCGCTCA TGGCCGTGGT GGTCTTCGTC 
GGAGTCAAAT ATGTCAACAA GCTGGCCCTG GTCTTCTTAG CCTGTGTTGT GCTTTCTATC 
CTGGCCATCT ATGCTGGTGT CATCAAGACA GCCTTCGCCC CACCTGACAT CCCGGTCTGC 
CTTCTAGGGA ACCGCACGCT GGCGAATCGC AACTTTGATA CCTGTGCCAA AATGCAGGTT 
GTCAGCAATG GCACAGTCAC CACCGCGCTC TGGCGCCTCT TCTGCAACGG CTCCAGCTTG 
GGTGCCTCCT GCGATGAGTA CTTTGTGCAG AACAATGTCA CTGAGATCCA GGGCATTCCT 
GGTGTGGCCA GTGGTGTCTT CCTGGATAAC CTGTGGAGCA CGTATTCGGA CAAGGGGGCA 
TTTGTGGAAA AGAAGGGCGT GTCCTCGGTG CCTGTGTCCG AGGAGAGCCG GCCTGGTGGA 
TTGCCATATG TCCTCACAGA CATCATGACC TACTTCACCA TGCTAGTCGG CATCTACTTC 
CCCTCCGTAA CGGGGATCAT GGCAGGATCC AACCGCTCCG GGGACCTCAA AGATGCCCAG 
AAGTCCATCC CAACAGGGAC CATTCTGGCC ATTGTGACTA CATCTTTCAT TTATCTTTCC 
TGCATTGTGC TGTTTGGGGC CTGCATCGAG GGTGTAGTCT TGCGAGATAA GTTTGGGGAG 
GCCCTGCAAG GGAACTTGGT CATTGGCATG CTGGCTTGGC CATCTCCCTG GGTCATCGTG 
ATTGGCTCCT TCTTCTCCAC CTGTGGTGCT GGCCTGCAGA GCCTAACTGG GGCACCCCGC 
CTGCTGCAGG CCATTGCACG CGACGGCATC ATCCCCTTCC TGCAGGTGTT TGGCCATGGA 
AAGGCCAACG GGGAGCCCAC GTGGGCCCTG CTGCTCACGG CCCTCATCTG TGAGACCGGC 
ATCCTCATCG CCTCCCTGGA CAGCGTGGCT CCCATCCTGT CCATGTTCTT CCTCATGTGC 
TACATGTTCG TCAACCTGGC CTGTGCTGTT CAGACCCTGC TGCGCACACC CAACTGGCGT 
CCACGTTTCA AGTTCTACCA CTGGACCCTC TCCTTCCTTG GGATGAGTCT CTGCCTTGCG 
CTCATGTTCA TCTGCTCCTG GTACTACGCC CTCTTCGCCA TGCTCATCGC GGGCTGCATC 
TACAAGTACA TCGAGTACCG AGGGGCTGAG AAGGAGTGGG GGGATGGCAT CAGGGGCCTG 
TCACTGAATG CTGCCCGCTA TGCCCTGCTG CGTGTGGAAC ATGGGCCCCC ACATACCAAG 
AACTGGAGGC CCCAGGTATT GGTGATGCTG AACCTGGACT CTGAGCAGTG TGTAAAGCAC 
CCCCGCCTGC TGTCCTTCAC CTCTCAGCTG AAGGCTGGCA AGGGCCTGAC CATCGTGGGA 
TCTGTGCTGG AGGGCACCTA CTTAGACAAG CATGTGGAGG CCCAGCGGGC TGAGGAGAAT 
ATCCGGTCTC TGATGAGTGC AGAGAAGATG AAAGGCTTCT GCCAGCTGGT GGTGTCGTCC 
AACCTGCGAG ACGGTGCATC CCACTTGATC CAGTCAGCTG GCCTTGGTGG CATGAAGCAC 
AACACTGTCC TCATGGCCTG GCCAGAGGCT TGGAAGCAGG CAGATAACCC TTTCTCCTGG 
AAGAACTTTG TGGACACTGT CCGTGACACT ACAGCAGCAC ATCAGGCACT GTTGGTGGCC 
AAGAACATCG ACTTGTTCCC GCAGAACCAG GAGCGCTTCA GCGACGGCAA CATCGATGTG 
TGGTGGATCG TGCACGATGG GGGGATGCTC ATGCTTCTGC CGTTCCTGCT GCGCCAGCAC 
AAGGTGTGGC GAAAGTGCCG GATGCGCATC TTCACGGTCG CCCAGGTGGA CGATAACAGC 
ATCCAGATGA AGAAGGACCT GCAGATGTTC CTGTACCATC TCAGGATCAG CGCCGAGGTG 
GAGGTGGTAG AGATGGTTGA AAATGACATT TCTGCATTCA CCTATGAGAA GACGCTAATG 
ATGGAGCAGA GGTCACAGAT GCTGAAACAG ATGCAGTTGT CAAAGAATGA GCGGGAGAGA 
GAGGCCCAGC TGATTCACGA CAGGAACACT GCATCCCACA CCGTAGCAAC GGCTAGAACT 
GAAGCCCCAC CAACACCTGA CAAAGTGCAG ATGACATGGA CGAAAGAGAA ACTCATTGCA 
GAGAAACACA GGAACAAGGA CACCGGCACA TCAGGCTTCA AAGACCTTTT CAGCCTAAAG 
CCAGAGTGGG GAAACCTGGA CCAGTCCAAC GTCAGAAGGA TGCATACTGC TGTGAAGCTC 
AATGGTGTGG TTCTCAACAA GTCCCAGGAT GCTCAACTCG TCCTGCTGAA TATGCCAGGC 
CCCCCAAAAA GTCGGCAGGG CGACGAGAAC TACATGGAAT TTCTTGAGGT CTTGACCGAA 
GGGCTGAACA GGGTCCTCCT GGTCAGGGGT GGTGGCCGGG AAGTCATCAC CATCTACTCC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017444580.1
CDS	82..3444
Translation	MSQRFVVIPAQSGTGDAGPRAEGGSGGSQRLCSDPDPDPDPDPDPDPSLAAALKTQEVDALPILRYCREPSRYGDGNPRENSPFINNVEVERESYFEGKNMALFEEEMDSNPMVSSLLNKLANYTNLSQGVVEHEEDEDSRRREIKAPRMGTFIGVYLPCLQNILGVILFLRLTWIVGAAGVLESFLIVAMCCTCTMLTAISMSAIATNGVVPAGGSYYMISRSLGPEFGGAVGLCFYLGTTFAGAMYILGTIEIFLTYISPSAAIFQAETADGEAAALLNNMRVYGSCALALMAVVVFVGVKYVNKLALVFLACVVLSILAIYAGVIKTAFAPPDIPVCLLGNRTLANRNFDTCAKMQVVSNGTVTTALWRLFCNGSSLGASCDEYFVQNNVTEIQGIPGVASGVFLDNLWSTYSDKGAFVEKKGVSSVPVSEESRPGGLPYVLTDIMTYFTMLVGIYFPSVTGIMAGSNRSGDLKDAQKSIPTGTILAIVTTSFIYLSCIVLFGACIEGVVLRDKFGEALQGNLVIGMLAWPSPWVIVIGSFFSTCGAGLQSLTGAPRLLQAIARDGIIPFLQVFGHGKANGEPTWALLLTALICETGILIASLDSVAPILSMFFLMCYMFVNLACAVQTLLRTPNWRPRFKFYHWTLSFLGMSLCLALMFICSWYYALFAMLIAGCIYKYIEYRGAEKEWGDGIRGLSLNAARYALLRVEHGPPHTKNWRPQVLVMLNLDSEQCVKHPRLLSFTSQLKAGKGLTIVGSVLEGTYLDKHVEAQRAEENIRSLMSAEKMKGFCQLVVSSNLRDGASHLIQSAGLGGMKHNTVLMAWPEAWKQADNPFSWKNFVDTVRDTTAAHQALLVAKNIDLFPQNQERFSDGNIDVWWIVHDGGMLMLLPFLLRQHKVWRKCRMRIFTVAQVDDNSIQMKKDLQMFLYHLRISAEVEVVEMVENDISAFTYEKTLMMEQRSQMLKQMQLSKNEREREAQLIHDRNTASHTVATARTEAPPTPDKVQMTWTKEKLIAEKHRNKDTGTSGFKDLFSLKPEWGNLDQSNVRRMHTAVKLNGVVLNKSQDAQLVLLNMPGPPKSRQGDENYMEFLEVLTEGLNRVLLVRGGGREVITIYS

Target ORF information:

RefSeq Version	XM_017589091.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus solute carrier family 12 member 7 (Slc12a7), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017589091.1

ORF Insert Sequence:

ATGTCCCAGC GCTTCGTGGT GATCCCAGCA CAGAGCGGGA CGGGGGACGC GGGACCCAGG 
GCAGAAGGTG GCAGCGGCGG CAGCCAGCGC CTGTGCTCGG ATCCCGACCC CGACCCCGAC 
CCTGACCCCG ACCCCGATCC CAGCCTGGCG GCCGCTCTGA AAACACAAGA AGTAGATGCG 
CTGCCCATTC TGCGCTACTG CCGCGAGCCC AGCCGCTATG GAGATGGAAA CCCCAGGGAG 
AACAGCCCAT TCATCAATAA TGTGGAGGTG GAAAGAGAGA GCTACTTCGA GGGGAAGAAC 
ATGGCGCTTT TTGAGGAAGA GATGGACAGC AACCCCATGG TATCATCACT GCTGAACAAG 
CTGGCCAACT ATACCAACCT GAGCCAGGGT GTGGTAGAGC ATGAGGAAGA TGAGGACAGC 
CGGAGGCGGG AGATCAAGGC CCCACGCATG GGCACCTTCA TCGGAGTCTA CCTACCGTGC 
CTGCAGAACA TCTTGGGTGT AATCCTTTTC CTGCGCCTGA CCTGGATTGT GGGGGCGGCT 
GGCGTTCTGG AGTCCTTCCT CATCGTGGCC ATGTGCTGCA CCTGTACAAT GCTGACAGCC 
ATCTCCATGA GTGCCATCGC TACCAACGGT GTGGTCCCAG CGGGAGGCTC ATACTACATG 
ATCTCCCGTT CGCTGGGGCC TGAGTTTGGA GGGGCTGTTG GCCTCTGCTT CTACTTGGGC 
ACAACGTTCG CAGGCGCCAT GTACATCCTG GGAACCATCG AGATCTTCCT GACCTACATC 
TCTCCAAGTG CAGCCATCTT CCAGGCAGAG ACAGCAGACG GCGAGGCGGC CGCGCTGCTG 
AACAACATGC GTGTGTATGG CAGCTGCGCA CTGGCGCTCA TGGCCGTGGT GGTCTTCGTC 
GGAGTCAAAT ATGTCAACAA GCTGGCCCTG GTCTTCTTAG CCTGTGTTGT GCTTTCTATC 
CTGGCCATCT ATGCTGGTGT CATCAAGACA GCCTTCGCCC CACCTGACAT CCCGGTCTGC 
CTTCTAGGGA ACCGCACGCT GGCGAATCGC AACTTTGATA CCTGTGCCAA AATGCAGGTT 
GTCAGCAATG GCACAGTCAC CACCGCGCTC TGGCGCCTCT TCTGCAACGG CTCCAGCTTG 
GGTGCCTCCT GCGATGAGTA CTTTGTGCAG AACAATGTCA CTGAGATCCA GGGCATTCCT 
GGTGTGGCCA GTGGTGTCTT CCTGGATAAC CTGTGGAGCA CGTATTCGGA CAAGGGGGCA 
TTTGTGGAAA AGAAGGGCGT GTCCTCGGTG CCTGTGTCCG AGGAGAGCCG GCCTGGTGGA 
TTGCCATATG TCCTCACAGA CATCATGACC TACTTCACCA TGCTAGTCGG CATCTACTTC 
CCCTCCGTAA CGGGGATCAT GGCAGGATCC AACCGCTCCG GGGACCTCAA AGATGCCCAG 
AAGTCCATCC CAACAGGGAC CATTCTGGCC ATTGTGACTA CATCTTTCAT TTATCTTTCC 
TGCATTGTGC TGTTTGGGGC CTGCATCGAG GGTGTAGTCT TGCGAGATAA GTTTGGGGAG 
GCCCTGCAAG GGAACTTGGT CATTGGCATG CTGGCTTGGC CATCTCCCTG GGTCATCGTG 
ATTGGCTCCT TCTTCTCCAC CTGTGGTGCT GGCCTGCAGA GCCTAACTGG GGCACCCCGC 
CTGCTGCAGG CCATTGCACG CGACGGCATC ATCCCCTTCC TGCAGGTGTT TGGCCATGGA 
AAGGCCAACG GGGAGCCCAC GTGGGCCCTG CTGCTCACGG CCCTCATCTG TGAGACCGGC 
ATCCTCATCG CCTCCCTGGA CAGCGTGGCT CCCATCCTGT CCATGTTCTT CCTCATGTGC 
TACATGTTCG TCAACCTGGC CTGTGCTGTT CAGACCCTGC TGCGCACACC CAACTGGCGT 
CCACGTTTCA AGTTCTACCA CTGGACCCTC TCCTTCCTTG GGATGAGTCT CTGCCTTGCG 
CTCATGTTCA TCTGCTCCTG GTACTACGCC CTCTTCGCCA TGCTCATCGC GGGCTGCATC 
TACAAGTACA TCGAGTACCG AGGGGCTGAG AAGGAGTGGG GGGATGGCAT CAGGGGCCTG 
TCACTGAATG CTGCCCGCTA TGCCCTGCTG CGTGTGGAAC ATGGGCCCCC ACATACCAAG 
AACTGGAGGC CCCAGGTATT GGTGATGCTG AACCTGGACT CTGAGCAGTG TGTAAAGCAC 
CCCCGCCTGC TGTCCTTCAC CTCTCAGCTG AAGGCTGGCA AGGGCCTGAC CATCGTGGGA 
TCTGTGCTGG AGGGCACCTA CTTAGACAAG CATGTGGAGG CCCAGCGGGC TGAGGAGAAT 
ATCCGGTCTC TGATGAGTGC AGAGAAGATG AAAGGCTTCT GCCAGCTGGT GGTGTCGTCC 
AACCTGCGAG ACGGTGCATC CCACTTGATC CAGTCAGCTG GCCTTGGTGG CATGAAGCAC 
AACACTGTCC TCATGGCCTG GCCAGAGGCT TGGAAGCAGG CAGATAACCC TTTCTCCTGG 
AAGAACTTTG TGGACACTGT CCGTGACACT ACAGCAGCAC ATCAGGCACT GTTGGTGGCC 
AAGAACATCG ACTTGTTCCC GCAGAACCAG GAGCGCTTCA GCGACGGCAA CATCGATGTG 
TGGTGGATCG TGCACGATGG GGGGATGCTC ATGCTTCTGC CGTTCCTGCT GCGCCAGCAC 
AAGGTGTGGC GAAAGTGCCG GATGCGCATC TTCACGGTCG CCCAGGTGGA CGATAACAGC 
ATCCAGATGA AGAAGGACCT GCAGATGTTC CTGTACCATC TCAGGATCAG CGCCGAGGTG 
GAGGTGGTAG AGATGGTTGA AAATGACATT TCTGCATTCA CCTATGAGAA GACGCTAATG 
ATGGAGCAGA GGTCACAGAT GCTGAAACAG ATGCAGTTGT CAAAGAATGA GCGGGAGAGA 
GAGGCCCAGC TGATTCACGA CAGGAACACT GCATCCCACA CCGTAGCAAC GGCTAGAACT 
GAAGCCCCAC CAACACCTGA CAAAGTGCAG ATGACATGGA CGAAAGAGAA ACTCATTGCA 
GAGAAACACA GGAACAAGGA CACCGGCACA TCAGGCTTCA AAGACCTTTT CAGCCTAAAG 
CCAGAGTGGG GAAACCTGGA CCAGTCCAAC GTCAGAAGGA TGCATACTGC TGTGAAGCTC 
AATGGTGTGG TTCTCAACAA GTCCCAGGAT GCTCAACTCG TCCTGCTGAA TATGCCAGGC 
CCCCCAAAAA GTCGGCAGGG CGACGAGAAC TACATGGAAT TTCTTGAGGT CTTGACCGAA 
GGGCTGAACA GGGTCCTCCT GGTCAGGGGT GGTGGCCGGG AAGTCATCAC CATCTACTCC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Slc12a7 ( XM_017589092.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017444581.1 Rattus norvegicus solute carrier family 12 member 7 (Slc12a7), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ORa56554
Clone ID Related Accession (Same CDS sequence)	XM_017589092.1
Accession Version	XM_017589092.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3267bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	solute carrier family 12 member 7 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCCACGA ACTTTACGGT GGTGCCGGTG GAGGCGCGCG CCGACGGCGC CGGGGACGAA 
GCTGCTGAGC GCACGGAAGA ACCCGGGTCT CCCGAGAGCG CGGATCCTGC CTGCCCTACG 
CCGGGAGATG GAAACCCCAG GGAGAACAGC CCATTCATCA ATAATGTGGA GGTGGAAAGA 
GAGAGCTACT TCGAGGGGAA GAACATGGCG CTTTTTGAGG AAGAGATGGA CAGCAACCCC 
ATGGTATCAT CACTGCTGAA CAAGCTGGCC AACTATACCA ACCTGAGCCA GGGTGTGGTA 
GAGCATGAGG AAGATGAGGA CAGCCGGAGG CGGGAGATCA AGGCCCCACG CATGGGCACC 
TTCATCGGAG TCTACCTACC GTGCCTGCAG AACATCTTGG GTGTAATCCT TTTCCTGCGC 
CTGACCTGGA TTGTGGGGGC GGCTGGCGTT CTGGAGTCCT TCCTCATCGT GGCCATGTGC 
TGCACCTGTA CAATGCTGAC AGCCATCTCC ATGAGTGCCA TCGCTACCAA CGGTGTGGTC 
CCAGCGGGAG GCTCATACTA CATGATCTCC CGTTCGCTGG GGCCTGAGTT TGGAGGGGCT 
GTTGGCCTCT GCTTCTACTT GGGCACAACG TTCGCAGGCG CCATGTACAT CCTGGGAACC 
ATCGAGATCT TCCTGACCTA CATCTCTCCA AGTGCAGCCA TCTTCCAGGC AGAGACAGCA 
GACGGCGAGG CGGCCGCGCT GCTGAACAAC ATGCGTGTGT ATGGCAGCTG CGCACTGGCG 
CTCATGGCCG TGGTGGTCTT CGTCGGAGTC AAATATGTCA ACAAGCTGGC CCTGGTCTTC 
TTAGCCTGTG TTGTGCTTTC TATCCTGGCC ATCTATGCTG GTGTCATCAA GACAGCCTTC 
GCCCCACCTG ACATCCCGGT CTGCCTTCTA GGGAACCGCA CGCTGGCGAA TCGCAACTTT 
GATACCTGTG CCAAAATGCA GGTTGTCAGC AATGGCACAG TCACCACCGC GCTCTGGCGC 
CTCTTCTGCA ACGGCTCCAG CTTGGGTGCC TCCTGCGATG AGTACTTTGT GCAGAACAAT 
GTCACTGAGA TCCAGGGCAT TCCTGGTGTG GCCAGTGGTG TCTTCCTGGA TAACCTGTGG 
AGCACGTATT CGGACAAGGG GGCATTTGTG GAAAAGAAGG GCGTGTCCTC GGTGCCTGTG 
TCCGAGGAGA GCCGGCCTGG TGGATTGCCA TATGTCCTCA CAGACATCAT GACCTACTTC 
ACCATGCTAG TCGGCATCTA CTTCCCCTCC GTAACGGGGA TCATGGCAGG ATCCAACCGC 
TCCGGGGACC TCAAAGATGC CCAGAAGTCC ATCCCAACAG GGACCATTCT GGCCATTGTG 
ACTACATCTT TCATTTATCT TTCCTGCATT GTGCTGTTTG GGGCCTGCAT CGAGGGTGTA 
GTCTTGCGAG ATAAGTTTGG GGAGGCCCTG CAAGGGAACT TGGTCATTGG CATGCTGGCT 
TGGCCATCTC CCTGGGTCAT CGTGATTGGC TCCTTCTTCT CCACCTGTGG TGCTGGCCTG 
CAGAGCCTAA CTGGGGCACC CCGCCTGCTG CAGGCCATTG CACGCGACGG CATCATCCCC 
TTCCTGCAGG TGTTTGGCCA TGGAAAGGCC AACGGGGAGC CCACGTGGGC CCTGCTGCTC 
ACGGCCCTCA TCTGTGAGAC CGGCATCCTC ATCGCCTCCC TGGACAGCGT GGCTCCCATC 
CTGTCCATGT TCTTCCTCAT GTGCTACATG TTCGTCAACC TGGCCTGTGC TGTTCAGACC 
CTGCTGCGCA CACCCAACTG GCGTCCACGT TTCAAGTTCT ACCACTGGAC CCTCTCCTTC 
CTTGGGATGA GTCTCTGCCT TGCGCTCATG TTCATCTGCT CCTGGTACTA CGCCCTCTTC 
GCCATGCTCA TCGCGGGCTG CATCTACAAG TACATCGAGT ACCGAGGGGC TGAGAAGGAG 
TGGGGGGATG GCATCAGGGG CCTGTCACTG AATGCTGCCC GCTATGCCCT GCTGCGTGTG 
GAACATGGGC CCCCACATAC CAAGAACTGG AGGCCCCAGG TATTGGTGAT GCTGAACCTG 
GACTCTGAGC AGTGTGTAAA GCACCCCCGC CTGCTGTCCT TCACCTCTCA GCTGAAGGCT 
GGCAAGGGCC TGACCATCGT GGGATCTGTG CTGGAGGGCA CCTACTTAGA CAAGCATGTG 
GAGGCCCAGC GGGCTGAGGA GAATATCCGG TCTCTGATGA GTGCAGAGAA GATGAAAGGC 
TTCTGCCAGC TGGTGGTGTC GTCCAACCTG CGAGACGGTG CATCCCACTT GATCCAGTCA 
GCTGGCCTTG GTGGCATGAA GCACAACACT GTCCTCATGG CCTGGCCAGA GGCTTGGAAG 
CAGGCAGATA ACCCTTTCTC CTGGAAGAAC TTTGTGGACA CTGTCCGTGA CACTACAGCA 
GCACATCAGG CACTGTTGGT GGCCAAGAAC ATCGACTTGT TCCCGCAGAA CCAGGAGCGC 
TTCAGCGACG GCAACATCGA TGTGTGGTGG ATCGTGCACG ATGGGGGGAT GCTCATGCTT 
CTGCCGTTCC TGCTGCGCCA GCACAAGGTG TGGCGAAAGT GCCGGATGCG CATCTTCACG 
GTCGCCCAGG TGGACGATAA CAGCATCCAG ATGAAGAAGG ACCTGCAGAT GTTCCTGTAC 
CATCTCAGGA TCAGCGCCGA GGTGGAGGTG GTAGAGATGG TTGAAAATGA CATTTCTGCA 
TTCACCTATG AGAAGACGCT AATGATGGAG CAGAGGTCAC AGATGCTGAA ACAGATGCAG 
TTGTCAAAGA ATGAGCGGGA GAGAGAGGCC CAGCTGATTC ACGACAGGAA CACTGCATCC 
CACACCGTAG CAACGGCTAG AACTGAAGCC CCACCAACAC CTGACAAAGT GCAGATGACA 
TGGACGAAAG AGAAACTCAT TGCAGAGAAA CACAGGAACA AGGACACCGG CACATCAGGC 
TTCAAAGACC TTTTCAGCCT AAAGCCAGAG TGGGGAAACC TGGACCAGTC CAACGTCAGA 
AGGATGCATA CTGCTGTGAA GCTCAATGGT GTGGTTCTCA ACAAGTCCCA GGATGCTCAA 
CTCGTCCTGC TGAATATGCC AGGCCCCCCA AAAAGTCGGC AGGGCGACGA GAACTACATG 
GAATTTCTTG AGGTCTTGAC CGAAGGGCTG AACAGGGTCC TCCTGGTCAG GGGTGGTGGC 
CGGGAAGTCA TCACCATCTA CTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017444581.1
CDS	2296..5562
Translation	MPTNFTVVPVEARADGAGDEAAERTEEPGSPESADPACPTPGDGNPRENSPFINNVEVERESYFEGKNMALFEEEMDSNPMVSSLLNKLANYTNLSQGVVEHEEDEDSRRREIKAPRMGTFIGVYLPCLQNILGVILFLRLTWIVGAAGVLESFLIVAMCCTCTMLTAISMSAIATNGVVPAGGSYYMISRSLGPEFGGAVGLCFYLGTTFAGAMYILGTIEIFLTYISPSAAIFQAETADGEAAALLNNMRVYGSCALALMAVVVFVGVKYVNKLALVFLACVVLSILAIYAGVIKTAFAPPDIPVCLLGNRTLANRNFDTCAKMQVVSNGTVTTALWRLFCNGSSLGASCDEYFVQNNVTEIQGIPGVASGVFLDNLWSTYSDKGAFVEKKGVSSVPVSEESRPGGLPYVLTDIMTYFTMLVGIYFPSVTGIMAGSNRSGDLKDAQKSIPTGTILAIVTTSFIYLSCIVLFGACIEGVVLRDKFGEALQGNLVIGMLAWPSPWVIVIGSFFSTCGAGLQSLTGAPRLLQAIARDGIIPFLQVFGHGKANGEPTWALLLTALICETGILIASLDSVAPILSMFFLMCYMFVNLACAVQTLLRTPNWRPRFKFYHWTLSFLGMSLCLALMFICSWYYALFAMLIAGCIYKYIEYRGAEKEWGDGIRGLSLNAARYALLRVEHGPPHTKNWRPQVLVMLNLDSEQCVKHPRLLSFTSQLKAGKGLTIVGSVLEGTYLDKHVEAQRAEENIRSLMSAEKMKGFCQLVVSSNLRDGASHLIQSAGLGGMKHNTVLMAWPEAWKQADNPFSWKNFVDTVRDTTAAHQALLVAKNIDLFPQNQERFSDGNIDVWWIVHDGGMLMLLPFLLRQHKVWRKCRMRIFTVAQVDDNSIQMKKDLQMFLYHLRISAEVEVVEMVENDISAFTYEKTLMMEQRSQMLKQMQLSKNEREREAQLIHDRNTASHTVATARTEAPPTPDKVQMTWTKEKLIAEKHRNKDTGTSGFKDLFSLKPEWGNLDQSNVRRMHTAVKLNGVVLNKSQDAQLVLLNMPGPPKSRQGDENYMEFLEVLTEGLNRVLLVRGGGREVITIYS

Target ORF information:

RefSeq Version	XM_017589092.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus solute carrier family 12 member 7 (Slc12a7), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017589092.1

ORF Insert Sequence:

ATGCCCACGA ACTTTACGGT GGTGCCGGTG GAGGCGCGCG CCGACGGCGC CGGGGACGAA 
GCTGCTGAGC GCACGGAAGA ACCCGGGTCT CCCGAGAGCG CGGATCCTGC CTGCCCTACG 
CCGGGAGATG GAAACCCCAG GGAGAACAGC CCATTCATCA ATAATGTGGA GGTGGAAAGA 
GAGAGCTACT TCGAGGGGAA GAACATGGCG CTTTTTGAGG AAGAGATGGA CAGCAACCCC 
ATGGTATCAT CACTGCTGAA CAAGCTGGCC AACTATACCA ACCTGAGCCA GGGTGTGGTA 
GAGCATGAGG AAGATGAGGA CAGCCGGAGG CGGGAGATCA AGGCCCCACG CATGGGCACC 
TTCATCGGAG TCTACCTACC GTGCCTGCAG AACATCTTGG GTGTAATCCT TTTCCTGCGC 
CTGACCTGGA TTGTGGGGGC GGCTGGCGTT CTGGAGTCCT TCCTCATCGT GGCCATGTGC 
TGCACCTGTA CAATGCTGAC AGCCATCTCC ATGAGTGCCA TCGCTACCAA CGGTGTGGTC 
CCAGCGGGAG GCTCATACTA CATGATCTCC CGTTCGCTGG GGCCTGAGTT TGGAGGGGCT 
GTTGGCCTCT GCTTCTACTT GGGCACAACG TTCGCAGGCG CCATGTACAT CCTGGGAACC 
ATCGAGATCT TCCTGACCTA CATCTCTCCA AGTGCAGCCA TCTTCCAGGC AGAGACAGCA 
GACGGCGAGG CGGCCGCGCT GCTGAACAAC ATGCGTGTGT ATGGCAGCTG CGCACTGGCG 
CTCATGGCCG TGGTGGTCTT CGTCGGAGTC AAATATGTCA ACAAGCTGGC CCTGGTCTTC 
TTAGCCTGTG TTGTGCTTTC TATCCTGGCC ATCTATGCTG GTGTCATCAA GACAGCCTTC 
GCCCCACCTG ACATCCCGGT CTGCCTTCTA GGGAACCGCA CGCTGGCGAA TCGCAACTTT 
GATACCTGTG CCAAAATGCA GGTTGTCAGC AATGGCACAG TCACCACCGC GCTCTGGCGC 
CTCTTCTGCA ACGGCTCCAG CTTGGGTGCC TCCTGCGATG AGTACTTTGT GCAGAACAAT 
GTCACTGAGA TCCAGGGCAT TCCTGGTGTG GCCAGTGGTG TCTTCCTGGA TAACCTGTGG 
AGCACGTATT CGGACAAGGG GGCATTTGTG GAAAAGAAGG GCGTGTCCTC GGTGCCTGTG 
TCCGAGGAGA GCCGGCCTGG TGGATTGCCA TATGTCCTCA CAGACATCAT GACCTACTTC 
ACCATGCTAG TCGGCATCTA CTTCCCCTCC GTAACGGGGA TCATGGCAGG ATCCAACCGC 
TCCGGGGACC TCAAAGATGC CCAGAAGTCC ATCCCAACAG GGACCATTCT GGCCATTGTG 
ACTACATCTT TCATTTATCT TTCCTGCATT GTGCTGTTTG GGGCCTGCAT CGAGGGTGTA 
GTCTTGCGAG ATAAGTTTGG GGAGGCCCTG CAAGGGAACT TGGTCATTGG CATGCTGGCT 
TGGCCATCTC CCTGGGTCAT CGTGATTGGC TCCTTCTTCT CCACCTGTGG TGCTGGCCTG 
CAGAGCCTAA CTGGGGCACC CCGCCTGCTG CAGGCCATTG CACGCGACGG CATCATCCCC 
TTCCTGCAGG TGTTTGGCCA TGGAAAGGCC AACGGGGAGC CCACGTGGGC CCTGCTGCTC 
ACGGCCCTCA TCTGTGAGAC CGGCATCCTC ATCGCCTCCC TGGACAGCGT GGCTCCCATC 
CTGTCCATGT TCTTCCTCAT GTGCTACATG TTCGTCAACC TGGCCTGTGC TGTTCAGACC 
CTGCTGCGCA CACCCAACTG GCGTCCACGT TTCAAGTTCT ACCACTGGAC CCTCTCCTTC 
CTTGGGATGA GTCTCTGCCT TGCGCTCATG TTCATCTGCT CCTGGTACTA CGCCCTCTTC 
GCCATGCTCA TCGCGGGCTG CATCTACAAG TACATCGAGT ACCGAGGGGC TGAGAAGGAG 
TGGGGGGATG GCATCAGGGG CCTGTCACTG AATGCTGCCC GCTATGCCCT GCTGCGTGTG 
GAACATGGGC CCCCACATAC CAAGAACTGG AGGCCCCAGG TATTGGTGAT GCTGAACCTG 
GACTCTGAGC AGTGTGTAAA GCACCCCCGC CTGCTGTCCT TCACCTCTCA GCTGAAGGCT 
GGCAAGGGCC TGACCATCGT GGGATCTGTG CTGGAGGGCA CCTACTTAGA CAAGCATGTG 
GAGGCCCAGC GGGCTGAGGA GAATATCCGG TCTCTGATGA GTGCAGAGAA GATGAAAGGC 
TTCTGCCAGC TGGTGGTGTC GTCCAACCTG CGAGACGGTG CATCCCACTT GATCCAGTCA 
GCTGGCCTTG GTGGCATGAA GCACAACACT GTCCTCATGG CCTGGCCAGA GGCTTGGAAG 
CAGGCAGATA ACCCTTTCTC CTGGAAGAAC TTTGTGGACA CTGTCCGTGA CACTACAGCA 
GCACATCAGG CACTGTTGGT GGCCAAGAAC ATCGACTTGT TCCCGCAGAA CCAGGAGCGC 
TTCAGCGACG GCAACATCGA TGTGTGGTGG ATCGTGCACG ATGGGGGGAT GCTCATGCTT 
CTGCCGTTCC TGCTGCGCCA GCACAAGGTG TGGCGAAAGT GCCGGATGCG CATCTTCACG 
GTCGCCCAGG TGGACGATAA CAGCATCCAG ATGAAGAAGG ACCTGCAGAT GTTCCTGTAC 
CATCTCAGGA TCAGCGCCGA GGTGGAGGTG GTAGAGATGG TTGAAAATGA CATTTCTGCA 
TTCACCTATG AGAAGACGCT AATGATGGAG CAGAGGTCAC AGATGCTGAA ACAGATGCAG 
TTGTCAAAGA ATGAGCGGGA GAGAGAGGCC CAGCTGATTC ACGACAGGAA CACTGCATCC 
CACACCGTAG CAACGGCTAG AACTGAAGCC CCACCAACAC CTGACAAAGT GCAGATGACA 
TGGACGAAAG AGAAACTCAT TGCAGAGAAA CACAGGAACA AGGACACCGG CACATCAGGC 
TTCAAAGACC TTTTCAGCCT AAAGCCAGAG TGGGGAAACC TGGACCAGTC CAACGTCAGA 
AGGATGCATA CTGCTGTGAA GCTCAATGGT GTGGTTCTCA ACAAGTCCCA GGATGCTCAA 
CTCGTCCTGC TGAATATGCC AGGCCCCCCA AAAAGTCGGC AGGGCGACGA GAACTACATG 
GAATTTCTTG AGGTCTTGAC CGAAGGGCTG AACAGGGTCC TCCTGGTCAG GGGTGGTGGC 
CGGGAAGTCA TCACCATCTA CTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Slc12a7 ( NM_001013144.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001013162.2 Rattus norvegicus solute carrier family 12 member 7 (Slc12a7), mRNA.

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CloneID	ORa03529
Clone ID Related Accession (Same CDS sequence)	NM_001013144.2
Accession Version	NM_001013144.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3252bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-23
Organism	Rattus norvegicus(Norway rat)
Product	solute carrier family 12 member 7
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AABR07071857.1. On Nov 13, 2010 this sequence version replaced NM_001013144.1. Sequence Note: This RefSeq record was created from genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record are predicted based on partial rat transcript alignments and on full-length homologous protein alignments with human NP_006589.2 and mouse NP_035520.1. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCCACGA ACTTTACGGT GGTGCCGGTG GAGGCGCGCG CCGACGGCGC CGGGGACGAA 
GCTGCTGAGC GCACGGAAGA ACCCGGGTCT CCCGAGAGCG CGGATCCTGC CTGCCCTACG 
CCGGGAGATG GAAACCCCAG GGAGAACAGC CCATTCATCA ATAATGTGGA GGTGGAAAGA 
GAGAGCTACT TCGAGGGGAA GAACATGGCG CTTTTTGAGG AAGAGATGGA CAGCAACCCC 
ATGGTATCAT CACTGCTGAA CAAGCTGGCC AACTATACCA ACCTGAGCCA GGGTGTGGTA 
GAGCATGAGG AAGATGAGGA CAGCCGGAGG CGGGAGATCA AGGCCCCACG CATGGGCACC 
TTCATCGGAG TCTACCTACC GTGCCTGCAG AACATCTTGG GTGTAATCCT TTTCCTGCGC 
CTGACCTGGA TTGTGGGGGC GGCTGGCGTT CTGGAGTCCT TCCTCATCGT GGCCATGTGC 
TGCACCTGTA CAATGCTGAC AGCCATCTCC ATGAGTGCCA TCGCTACCAA CGGTGTGGTC 
CCAGCGGGAG GCTCATACTA CATGATCTCC CGTTCGCTGG GGCCTGAGTT TGGAGGGGCT 
GTTGGCCTCT GCTTCTACTT GGGCACAACG TTCGCAGGCG CCATGTACAT CCTGGGAACC 
ATCGAGATCT TCCTGACCTA CATCTCTCCA AGTGCAGCCA TCTTCCAGGC AGAGACAGCA 
GACGGCGAGG CGGCCGCGCT GCTGAACAAC ATGCGTGTGT ATGGCAGCTG CGCACTGGCG 
CTCATGGCCG TGGTGGTCTT CGTCGGAGTC AAATATGTCA ACAAGCTGGC CCTGGTCTTC 
TTAGCCTGTG TTGTGCTTTC TATCCTGGCC ATCTATGCTG GTGTCATCAA GACAGCCTTC 
GCCCCACCTG ACATCCCGGT CTGCCTTCTA GGGAACCGCA CGCTGGCGAA TCGCAACTTT 
GATACCTGTG CCAAAATGCA GGTTGTCAGC AATGGCACAG TCACCACCGC GCTCTGGCGC 
CTCTTCTGCA ACGGCTCCAG CTTGGGTGCC TCCTGCGATG AGTACTTTGT GCAGAACAAT 
GTCACTGAGA TCCAGGGCAT TCCTGGTGTG GCCAGTGGTG TCTTCCTGGA TAACCTGTGG 
AGCACGTATT CGGACAAGGG GGCATTTGTG GAAAAGAAGG GCGTGTCCTC GGTGCCTGTG 
TCCGAGGAGA GCCGGCCTGG TGGATTGCCA TATGTCCTCA CAGACATCAT GACCTACTTC 
ACCATGCTAG TCGGCATCTA CTTCCCCTCC GTAACGGGGA TCATGGCAGG ATCCAACCGC 
TCCGGGGACC TCAAAGATGC CCAGAAGTCC ATCCCAACAG GGACCATTCT GGCCATTGTG 
ACTACATCTT TCATTTATCT TTCCTGCATT GTGCTGTTTG GGGCCTGCAT CGAGGGTGTA 
GTCTTGCGAG ATAAGTTTGG GGAGGCCCTG CAAGGGAACT TGGTCATTGG CATGCTGGCT 
TGGCCATCTC CCTGGGTCAT CGTGATTGGC TCCTTCTTCT CCACCTGTGG TGCTGGCCTG 
CAGAGCCTAA CTGGGGCACC CCGCCTGCTG CAGGCCATTG CACGCGACGG CATCATCCCC 
TTCCTGCAGG TGTTTGGCCA TGGAAAGGCC AACGGGGAGC CCACGTGGGC CCTGCTGCTC 
ACGGCCCTCA TCTGTGAGAC CGGCATCCTC ATCGCCTCCC TGGACAGCGT GGCTCCCATC 
CTGTCCATGT TCTTCCTCAT GTGCTACATG TTCGTCAACC TGGCCTGTGC TGTTCAGACC 
CTGCTGCGCA CACCCAACTG GCGTCCACGT TTCAAGTTCT ACCACTGGAC CCTCTCCTTC 
CTTGGGATGA GTCTCTGCCT TGCGCTCATG TTCATCTGCT CCTGGTACTA CGCCCTCTTC 
GCCATGCTCA TCGCGGGCTG CATCTACAAG TACATCGAGT ACCGAGGGGC TGAGAAGGAG 
TGGGGGGATG GCATCAGGGG CCTGTCACTG AATGCTGCCC GCTATGCCCT GCTGCGTGTG 
GAACATGGGC CCCCACATAC CAAGAACTGG AGGCCCCAGG TATTGGTGAT GCTGAACCTG 
GACTCTGAGC AGTGTGTAAA GCACCCCCGC CTGCTGTCCT TCACCTCTCA GCTGAAGGCT 
GGCAAGGGCC TGACCATCGT GGGATCTGTG CTGGAGGGCA CCTACTTAGA CAAGCATGTG 
GAGGCCCAGC GGGCTGAGGA GAATATCCGG TCTCTGATGA GTGCAGAGAA GATGAAAGGC 
TTCTGCCAGC TGGTGGTGTC GTCCAACCTG CGAGACGGTG CATCCCACTT GATCCAGTCA 
GCTGGCCTTG GTGGCATGAA GCACAACACT GTCCTCATGG CCTGGCCAGA GGCTTGGAAG 
CAGGCAGATA ACCCTTTCTC CTGGAAGAAC TTTGTGGACA CTGTCCGTGA CACTACAGCA 
GCACATCAGG CACTGTTGGT GGCCAAGAAC ATCGACTTGT TCCCGCAGAA CCAGGAGCGC 
TTCAGCGACG GCAACATCGA TGTGTGGTGG ATCGTGCACG ATGGGGGGAT GCTCATGCTT 
CTGCCGTTCC TGCTGCGCCA GCACAAGGTG TGGCGAAAGT GCCGGATGCG CATCTTCACG 
GTCGCCCAGG TGGACGATAA CAGCATCCAG ATGAAGAAGG ACCTGCAGAT GTTCCTGTAC 
CATCTCAGGA TCAGCGCCGA GGTGGAGGTG GTAGAGATGG TTGAAAATGA CATTTCTGCA 
TTCACCTATG AGAAGACGCT AATGATGGAG CAGAGGTCAC AGATGCTGAA ACAGATGCAG 
TTGTCAAAGA ATGAGCGGGA GAGAGAGGCC CAGCTGATTC ACGACAGGAA CACTGCATCC 
CACACCGTAG CAACGGCTAG AACTGAAGCC CCACCAACAC CTGACAAAGT GCAGATGACA 
TGGACGAAAG AGAAACTCAT TGCAGAGAAA CACAGGAACA AGGACACCGG CACATCAGGC 
TTCAAAGACC TTTTCAGCCT AAAGCCGGAC CAGTCCAACG TCAGAAGGAT GCATACTGCT 
GTGAAGCTCA ATGGTGTGGT TCTCAACAAG TCCCAGGATG CTCAACTCGT CCTGCTGAAT 
ATGCCAGGCC CCCCAAAAAG TCGGCAGGGC GACGAGAACT ACATGGAATT TCTTGAGGTC 
TTGACCGAAG GGCTGAACAG GGTCCTCCTG GTCAGGGGTG GTGGCCGGGA AGTCATCACC 
ATCTACTCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001013162.2
CDS	49..3300
Translation	MPTNFTVVPVEARADGAGDEAAERTEEPGSPESADPACPTPGDGNPRENSPFINNVEVERESYFEGKNMALFEEEMDSNPMVSSLLNKLANYTNLSQGVVEHEEDEDSRRREIKAPRMGTFIGVYLPCLQNILGVILFLRLTWIVGAAGVLESFLIVAMCCTCTMLTAISMSAIATNGVVPAGGSYYMISRSLGPEFGGAVGLCFYLGTTFAGAMYILGTIEIFLTYISPSAAIFQAETADGEAAALLNNMRVYGSCALALMAVVVFVGVKYVNKLALVFLACVVLSILAIYAGVIKTAFAPPDIPVCLLGNRTLANRNFDTCAKMQVVSNGTVTTALWRLFCNGSSLGASCDEYFVQNNVTEIQGIPGVASGVFLDNLWSTYSDKGAFVEKKGVSSVPVSEESRPGGLPYVLTDIMTYFTMLVGIYFPSVTGIMAGSNRSGDLKDAQKSIPTGTILAIVTTSFIYLSCIVLFGACIEGVVLRDKFGEALQGNLVIGMLAWPSPWVIVIGSFFSTCGAGLQSLTGAPRLLQAIARDGIIPFLQVFGHGKANGEPTWALLLTALICETGILIASLDSVAPILSMFFLMCYMFVNLACAVQTLLRTPNWRPRFKFYHWTLSFLGMSLCLALMFICSWYYALFAMLIAGCIYKYIEYRGAEKEWGDGIRGLSLNAARYALLRVEHGPPHTKNWRPQVLVMLNLDSEQCVKHPRLLSFTSQLKAGKGLTIVGSVLEGTYLDKHVEAQRAEENIRSLMSAEKMKGFCQLVVSSNLRDGASHLIQSAGLGGMKHNTVLMAWPEAWKQADNPFSWKNFVDTVRDTTAAHQALLVAKNIDLFPQNQERFSDGNIDVWWIVHDGGMLMLLPFLLRQHKVWRKCRMRIFTVAQVDDNSIQMKKDLQMFLYHLRISAEVEVVEMVENDISAFTYEKTLMMEQRSQMLKQMQLSKNEREREAQLIHDRNTASHTVATARTEAPPTPDKVQMTWTKEKLIAEKHRNKDTGTSGFKDLFSLKPDQSNVRRMHTAVKLNGVVLNKSQDAQLVLLNMPGPPKSRQGDENYMEFLEVLTEGLNRVLLVRGGGREVITIYS

Target ORF information:

RefSeq Version	NM_001013144.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus solute carrier family 12 member 7 (Slc12a7), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001013144.2

ORF Insert Sequence:

ATGCCCACGA ACTTTACGGT GGTGCCGGTG GAGGCGCGCG CCGACGGCGC CGGGGACGAA 
GCTGCTGAGC GCACGGAAGA ACCCGGGTCT CCCGAGAGCG CGGATCCTGC CTGCCCTACG 
CCGGGAGATG GAAACCCCAG GGAGAACAGC CCATTCATCA ATAATGTGGA GGTGGAAAGA 
GAGAGCTACT TCGAGGGGAA GAACATGGCG CTTTTTGAGG AAGAGATGGA CAGCAACCCC 
ATGGTATCAT CACTGCTGAA CAAGCTGGCC AACTATACCA ACCTGAGCCA GGGTGTGGTA 
GAGCATGAGG AAGATGAGGA CAGCCGGAGG CGGGAGATCA AGGCCCCACG CATGGGCACC 
TTCATCGGAG TCTACCTACC GTGCCTGCAG AACATCTTGG GTGTAATCCT TTTCCTGCGC 
CTGACCTGGA TTGTGGGGGC GGCTGGCGTT CTGGAGTCCT TCCTCATCGT GGCCATGTGC 
TGCACCTGTA CAATGCTGAC AGCCATCTCC ATGAGTGCCA TCGCTACCAA CGGTGTGGTC 
CCAGCGGGAG GCTCATACTA CATGATCTCC CGTTCGCTGG GGCCTGAGTT TGGAGGGGCT 
GTTGGCCTCT GCTTCTACTT GGGCACAACG TTCGCAGGCG CCATGTACAT CCTGGGAACC 
ATCGAGATCT TCCTGACCTA CATCTCTCCA AGTGCAGCCA TCTTCCAGGC AGAGACAGCA 
GACGGCGAGG CGGCCGCGCT GCTGAACAAC ATGCGTGTGT ATGGCAGCTG CGCACTGGCG 
CTCATGGCCG TGGTGGTCTT CGTCGGAGTC AAATATGTCA ACAAGCTGGC CCTGGTCTTC 
TTAGCCTGTG TTGTGCTTTC TATCCTGGCC ATCTATGCTG GTGTCATCAA GACAGCCTTC 
GCCCCACCTG ACATCCCGGT CTGCCTTCTA GGGAACCGCA CGCTGGCGAA TCGCAACTTT 
GATACCTGTG CCAAAATGCA GGTTGTCAGC AATGGCACAG TCACCACCGC GCTCTGGCGC 
CTCTTCTGCA ACGGCTCCAG CTTGGGTGCC TCCTGCGATG AGTACTTTGT GCAGAACAAT 
GTCACTGAGA TCCAGGGCAT TCCTGGTGTG GCCAGTGGTG TCTTCCTGGA TAACCTGTGG 
AGCACGTATT CGGACAAGGG GGCATTTGTG GAAAAGAAGG GCGTGTCCTC GGTGCCTGTG 
TCCGAGGAGA GCCGGCCTGG TGGATTGCCA TATGTCCTCA CAGACATCAT GACCTACTTC 
ACCATGCTAG TCGGCATCTA CTTCCCCTCC GTAACGGGGA TCATGGCAGG ATCCAACCGC 
TCCGGGGACC TCAAAGATGC CCAGAAGTCC ATCCCAACAG GGACCATTCT GGCCATTGTG 
ACTACATCTT TCATTTATCT TTCCTGCATT GTGCTGTTTG GGGCCTGCAT CGAGGGTGTA 
GTCTTGCGAG ATAAGTTTGG GGAGGCCCTG CAAGGGAACT TGGTCATTGG CATGCTGGCT 
TGGCCATCTC CCTGGGTCAT CGTGATTGGC TCCTTCTTCT CCACCTGTGG TGCTGGCCTG 
CAGAGCCTAA CTGGGGCACC CCGCCTGCTG CAGGCCATTG CACGCGACGG CATCATCCCC 
TTCCTGCAGG TGTTTGGCCA TGGAAAGGCC AACGGGGAGC CCACGTGGGC CCTGCTGCTC 
ACGGCCCTCA TCTGTGAGAC CGGCATCCTC ATCGCCTCCC TGGACAGCGT GGCTCCCATC 
CTGTCCATGT TCTTCCTCAT GTGCTACATG TTCGTCAACC TGGCCTGTGC TGTTCAGACC 
CTGCTGCGCA CACCCAACTG GCGTCCACGT TTCAAGTTCT ACCACTGGAC CCTCTCCTTC 
CTTGGGATGA GTCTCTGCCT TGCGCTCATG TTCATCTGCT CCTGGTACTA CGCCCTCTTC 
GCCATGCTCA TCGCGGGCTG CATCTACAAG TACATCGAGT ACCGAGGGGC TGAGAAGGAG 
TGGGGGGATG GCATCAGGGG CCTGTCACTG AATGCTGCCC GCTATGCCCT GCTGCGTGTG 
GAACATGGGC CCCCACATAC CAAGAACTGG AGGCCCCAGG TATTGGTGAT GCTGAACCTG 
GACTCTGAGC AGTGTGTAAA GCACCCCCGC CTGCTGTCCT TCACCTCTCA GCTGAAGGCT 
GGCAAGGGCC TGACCATCGT GGGATCTGTG CTGGAGGGCA CCTACTTAGA CAAGCATGTG 
GAGGCCCAGC GGGCTGAGGA GAATATCCGG TCTCTGATGA GTGCAGAGAA GATGAAAGGC 
TTCTGCCAGC TGGTGGTGTC GTCCAACCTG CGAGACGGTG CATCCCACTT GATCCAGTCA 
GCTGGCCTTG GTGGCATGAA GCACAACACT GTCCTCATGG CCTGGCCAGA GGCTTGGAAG 
CAGGCAGATA ACCCTTTCTC CTGGAAGAAC TTTGTGGACA CTGTCCGTGA CACTACAGCA 
GCACATCAGG CACTGTTGGT GGCCAAGAAC ATCGACTTGT TCCCGCAGAA CCAGGAGCGC 
TTCAGCGACG GCAACATCGA TGTGTGGTGG ATCGTGCACG ATGGGGGGAT GCTCATGCTT 
CTGCCGTTCC TGCTGCGCCA GCACAAGGTG TGGCGAAAGT GCCGGATGCG CATCTTCACG 
GTCGCCCAGG TGGACGATAA CAGCATCCAG ATGAAGAAGG ACCTGCAGAT GTTCCTGTAC 
CATCTCAGGA TCAGCGCCGA GGTGGAGGTG GTAGAGATGG TTGAAAATGA CATTTCTGCA 
TTCACCTATG AGAAGACGCT AATGATGGAG CAGAGGTCAC AGATGCTGAA ACAGATGCAG 
TTGTCAAAGA ATGAGCGGGA GAGAGAGGCC CAGCTGATTC ACGACAGGAA CACTGCATCC 
CACACCGTAG CAACGGCTAG AACTGAAGCC CCACCAACAC CTGACAAAGT GCAGATGACA 
TGGACGAAAG AGAAACTCAT TGCAGAGAAA CACAGGAACA AGGACACCGG CACATCAGGC 
TTCAAAGACC TTTTCAGCCT AAAGCCGGAC CAGTCCAACG TCAGAAGGAT GCATACTGCT 
GTGAAGCTCA ATGGTGTGGT TCTCAACAAG TCCCAGGATG CTCAACTCGT CCTGCTGAAT 
ATGCCAGGCC CCCCAAAAAG TCGGCAGGGC GACGAGAACT ACATGGAATT TCTTGAGGTC 
TTGACCGAAG GGCTGAACAG GGTCCTCCTG GTCAGGGGTG GTGGCCGGGA AGTCATCACC 
ATCTACTCCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Slc12a7 ( XM_006227789.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006227851.1 Rattus norvegicus solute carrier family 12 member 7 (Slc12a7), transcript variant X2, mRNA.

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CloneID	ORa22096
Clone ID Related Accession (Same CDS sequence)	XM_006227789.3
Accession Version	XM_006227789.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3348bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	solute carrier family 12 member 7 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006227789.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCCAGC GCTTCGTGGT GATCCCAGCA CAGAGCGGGA CGGGGGACGC GGGACCCAGG 
GCAGAAGGTG GCAGCGGCGG CAGCCAGCGC CTGTGCTCGG ATCCCGACCC CGACCCCGAC 
CCTGACCCCG ACCCCGATCC CAGCCTGGCG GCCGCTCTGA AAACACAAGA AGTAGATGCG 
CTGCCCATTC TGCGCTACTG CCGCGAGCCC AGCCGCTATG GAGATGGAAA CCCCAGGGAG 
AACAGCCCAT TCATCAATAA TGTGGAGGTG GAAAGAGAGA GCTACTTCGA GGGGAAGAAC 
ATGGCGCTTT TTGAGGAAGA GATGGACAGC AACCCCATGG TATCATCACT GCTGAACAAG 
CTGGCCAACT ATACCAACCT GAGCCAGGGT GTGGTAGAGC ATGAGGAAGA TGAGGACAGC 
CGGAGGCGGG AGATCAAGGC CCCACGCATG GGCACCTTCA TCGGAGTCTA CCTACCGTGC 
CTGCAGAACA TCTTGGGTGT AATCCTTTTC CTGCGCCTGA CCTGGATTGT GGGGGCGGCT 
GGCGTTCTGG AGTCCTTCCT CATCGTGGCC ATGTGCTGCA CCTGTACAAT GCTGACAGCC 
ATCTCCATGA GTGCCATCGC TACCAACGGT GTGGTCCCAG CGGGAGGCTC ATACTACATG 
ATCTCCCGTT CGCTGGGGCC TGAGTTTGGA GGGGCTGTTG GCCTCTGCTT CTACTTGGGC 
ACAACGTTCG CAGGCGCCAT GTACATCCTG GGAACCATCG AGATCTTCCT GACCTACATC 
TCTCCAAGTG CAGCCATCTT CCAGGCAGAG ACAGCAGACG GCGAGGCGGC CGCGCTGCTG 
AACAACATGC GTGTGTATGG CAGCTGCGCA CTGGCGCTCA TGGCCGTGGT GGTCTTCGTC 
GGAGTCAAAT ATGTCAACAA GCTGGCCCTG GTCTTCTTAG CCTGTGTTGT GCTTTCTATC 
CTGGCCATCT ATGCTGGTGT CATCAAGACA GCCTTCGCCC CACCTGACAT CCCGGTCTGC 
CTTCTAGGGA ACCGCACGCT GGCGAATCGC AACTTTGATA CCTGTGCCAA AATGCAGGTT 
GTCAGCAATG GCACAGTCAC CACCGCGCTC TGGCGCCTCT TCTGCAACGG CTCCAGCTTG 
GGTGCCTCCT GCGATGAGTA CTTTGTGCAG AACAATGTCA CTGAGATCCA GGGCATTCCT 
GGTGTGGCCA GTGGTGTCTT CCTGGATAAC CTGTGGAGCA CGTATTCGGA CAAGGGGGCA 
TTTGTGGAAA AGAAGGGCGT GTCCTCGGTG CCTGTGTCCG AGGAGAGCCG GCCTGGTGGA 
TTGCCATATG TCCTCACAGA CATCATGACC TACTTCACCA TGCTAGTCGG CATCTACTTC 
CCCTCCGTAA CGGGGATCAT GGCAGGATCC AACCGCTCCG GGGACCTCAA AGATGCCCAG 
AAGTCCATCC CAACAGGGAC CATTCTGGCC ATTGTGACTA CATCTTTCAT TTATCTTTCC 
TGCATTGTGC TGTTTGGGGC CTGCATCGAG GGTGTAGTCT TGCGAGATAA GTTTGGGGAG 
GCCCTGCAAG GGAACTTGGT CATTGGCATG CTGGCTTGGC CATCTCCCTG GGTCATCGTG 
ATTGGCTCCT TCTTCTCCAC CTGTGGTGCT GGCCTGCAGA GCCTAACTGG GGCACCCCGC 
CTGCTGCAGG CCATTGCACG CGACGGCATC ATCCCCTTCC TGCAGGTGTT TGGCCATGGA 
AAGGCCAACG GGGAGCCCAC GTGGGCCCTG CTGCTCACGG CCCTCATCTG TGAGACCGGC 
ATCCTCATCG CCTCCCTGGA CAGCGTGGCT CCCATCCTGT CCATGTTCTT CCTCATGTGC 
TACATGTTCG TCAACCTGGC CTGTGCTGTT CAGACCCTGC TGCGCACACC CAACTGGCGT 
CCACGTTTCA AGTTCTACCA CTGGACCCTC TCCTTCCTTG GGATGAGTCT CTGCCTTGCG 
CTCATGTTCA TCTGCTCCTG GTACTACGCC CTCTTCGCCA TGCTCATCGC GGGCTGCATC 
TACAAGTACA TCGAGTACCG AGGGGCTGAG AAGGAGTGGG GGGATGGCAT CAGGGGCCTG 
TCACTGAATG CTGCCCGCTA TGCCCTGCTG CGTGTGGAAC ATGGGCCCCC ACATACCAAG 
AACTGGAGGC CCCAGGTATT GGTGATGCTG AACCTGGACT CTGAGCAGTG TGTAAAGCAC 
CCCCGCCTGC TGTCCTTCAC CTCTCAGCTG AAGGCTGGCA AGGGCCTGAC CATCGTGGGA 
TCTGTGCTGG AGGGCACCTA CTTAGACAAG CATGTGGAGG CCCAGCGGGC TGAGGAGAAT 
ATCCGGTCTC TGATGAGTGC AGAGAAGATG AAAGGCTTCT GCCAGCTGGT GGTGTCGTCC 
AACCTGCGAG ACGGTGCATC CCACTTGATC CAGTCAGCTG GCCTTGGTGG CATGAAGCAC 
AACACTGTCC TCATGGCCTG GCCAGAGGCT TGGAAGCAGG CAGATAACCC TTTCTCCTGG 
AAGAACTTTG TGGACACTGT CCGTGACACT ACAGCAGCAC ATCAGGCACT GTTGGTGGCC 
AAGAACATCG ACTTGTTCCC GCAGAACCAG GAGCGCTTCA GCGACGGCAA CATCGATGTG 
TGGTGGATCG TGCACGATGG GGGGATGCTC ATGCTTCTGC CGTTCCTGCT GCGCCAGCAC 
AAGGTGTGGC GAAAGTGCCG GATGCGCATC TTCACGGTCG CCCAGGTGGA CGATAACAGC 
ATCCAGATGA AGAAGGACCT GCAGATGTTC CTGTACCATC TCAGGATCAG CGCCGAGGTG 
GAGGTGGTAG AGATGGTTGA AAATGACATT TCTGCATTCA CCTATGAGAA GACGCTAATG 
ATGGAGCAGA GGTCACAGAT GCTGAAACAG ATGCAGTTGT CAAAGAATGA GCGGGAGAGA 
GAGGCCCAGC TGATTCACGA CAGGAACACT GCATCCCACA CCGTAGCAAC GGCTAGAACT 
GAAGCCCCAC CAACACCTGA CAAAGTGCAG ATGACATGGA CGAAAGAGAA ACTCATTGCA 
GAGAAACACA GGAACAAGGA CACCGGCACA TCAGGCTTCA AAGACCTTTT CAGCCTAAAG 
CCGGACCAGT CCAACGTCAG AAGGATGCAT ACTGCTGTGA AGCTCAATGG TGTGGTTCTC 
AACAAGTCCC AGGATGCTCA ACTCGTCCTG CTGAATATGC CAGGCCCCCC AAAAAGTCGG 
CAGGGCGACG AGAACTACAT GGAATTTCTT GAGGTCTTGA CCGAAGGGCT GAACAGGGTC 
CTCCTGGTCA GGGGTGGTGG CCGGGAAGTC ATCACCATCT ACTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006227851.1
CDS	82..3429
Translation	MSQRFVVIPAQSGTGDAGPRAEGGSGGSQRLCSDPDPDPDPDPDPDPSLAAALKTQEVDALPILRYCREPSRYGDGNPRENSPFINNVEVERESYFEGKNMALFEEEMDSNPMVSSLLNKLANYTNLSQGVVEHEEDEDSRRREIKAPRMGTFIGVYLPCLQNILGVILFLRLTWIVGAAGVLESFLIVAMCCTCTMLTAISMSAIATNGVVPAGGSYYMISRSLGPEFGGAVGLCFYLGTTFAGAMYILGTIEIFLTYISPSAAIFQAETADGEAAALLNNMRVYGSCALALMAVVVFVGVKYVNKLALVFLACVVLSILAIYAGVIKTAFAPPDIPVCLLGNRTLANRNFDTCAKMQVVSNGTVTTALWRLFCNGSSLGASCDEYFVQNNVTEIQGIPGVASGVFLDNLWSTYSDKGAFVEKKGVSSVPVSEESRPGGLPYVLTDIMTYFTMLVGIYFPSVTGIMAGSNRSGDLKDAQKSIPTGTILAIVTTSFIYLSCIVLFGACIEGVVLRDKFGEALQGNLVIGMLAWPSPWVIVIGSFFSTCGAGLQSLTGAPRLLQAIARDGIIPFLQVFGHGKANGEPTWALLLTALICETGILIASLDSVAPILSMFFLMCYMFVNLACAVQTLLRTPNWRPRFKFYHWTLSFLGMSLCLALMFICSWYYALFAMLIAGCIYKYIEYRGAEKEWGDGIRGLSLNAARYALLRVEHGPPHTKNWRPQVLVMLNLDSEQCVKHPRLLSFTSQLKAGKGLTIVGSVLEGTYLDKHVEAQRAEENIRSLMSAEKMKGFCQLVVSSNLRDGASHLIQSAGLGGMKHNTVLMAWPEAWKQADNPFSWKNFVDTVRDTTAAHQALLVAKNIDLFPQNQERFSDGNIDVWWIVHDGGMLMLLPFLLRQHKVWRKCRMRIFTVAQVDDNSIQMKKDLQMFLYHLRISAEVEVVEMVENDISAFTYEKTLMMEQRSQMLKQMQLSKNEREREAQLIHDRNTASHTVATARTEAPPTPDKVQMTWTKEKLIAEKHRNKDTGTSGFKDLFSLKPDQSNVRRMHTAVKLNGVVLNKSQDAQLVLLNMPGPPKSRQGDENYMEFLEVLTEGLNRVLLVRGGGREVITIYS

Target ORF information:

RefSeq Version	XM_006227789.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus solute carrier family 12 member 7 (Slc12a7), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006227789.3

ORF Insert Sequence:

ATGTCCCAGC GCTTCGTGGT GATCCCAGCA CAGAGCGGGA CGGGGGACGC GGGACCCAGG 
GCAGAAGGTG GCAGCGGCGG CAGCCAGCGC CTGTGCTCGG ATCCCGACCC CGACCCCGAC 
CCTGACCCCG ACCCCGATCC CAGCCTGGCG GCCGCTCTGA AAACACAAGA AGTAGATGCG 
CTGCCCATTC TGCGCTACTG CCGCGAGCCC AGCCGCTATG GAGATGGAAA CCCCAGGGAG 
AACAGCCCAT TCATCAATAA TGTGGAGGTG GAAAGAGAGA GCTACTTCGA GGGGAAGAAC 
ATGGCGCTTT TTGAGGAAGA GATGGACAGC AACCCCATGG TATCATCACT GCTGAACAAG 
CTGGCCAACT ATACCAACCT GAGCCAGGGT GTGGTAGAGC ATGAGGAAGA TGAGGACAGC 
CGGAGGCGGG AGATCAAGGC CCCACGCATG GGCACCTTCA TCGGAGTCTA CCTACCGTGC 
CTGCAGAACA TCTTGGGTGT AATCCTTTTC CTGCGCCTGA CCTGGATTGT GGGGGCGGCT 
GGCGTTCTGG AGTCCTTCCT CATCGTGGCC ATGTGCTGCA CCTGTACAAT GCTGACAGCC 
ATCTCCATGA GTGCCATCGC TACCAACGGT GTGGTCCCAG CGGGAGGCTC ATACTACATG 
ATCTCCCGTT CGCTGGGGCC TGAGTTTGGA GGGGCTGTTG GCCTCTGCTT CTACTTGGGC 
ACAACGTTCG CAGGCGCCAT GTACATCCTG GGAACCATCG AGATCTTCCT GACCTACATC 
TCTCCAAGTG CAGCCATCTT CCAGGCAGAG ACAGCAGACG GCGAGGCGGC CGCGCTGCTG 
AACAACATGC GTGTGTATGG CAGCTGCGCA CTGGCGCTCA TGGCCGTGGT GGTCTTCGTC 
GGAGTCAAAT ATGTCAACAA GCTGGCCCTG GTCTTCTTAG CCTGTGTTGT GCTTTCTATC 
CTGGCCATCT ATGCTGGTGT CATCAAGACA GCCTTCGCCC CACCTGACAT CCCGGTCTGC 
CTTCTAGGGA ACCGCACGCT GGCGAATCGC AACTTTGATA CCTGTGCCAA AATGCAGGTT 
GTCAGCAATG GCACAGTCAC CACCGCGCTC TGGCGCCTCT TCTGCAACGG CTCCAGCTTG 
GGTGCCTCCT GCGATGAGTA CTTTGTGCAG AACAATGTCA CTGAGATCCA GGGCATTCCT 
GGTGTGGCCA GTGGTGTCTT CCTGGATAAC CTGTGGAGCA CGTATTCGGA CAAGGGGGCA 
TTTGTGGAAA AGAAGGGCGT GTCCTCGGTG CCTGTGTCCG AGGAGAGCCG GCCTGGTGGA 
TTGCCATATG TCCTCACAGA CATCATGACC TACTTCACCA TGCTAGTCGG CATCTACTTC 
CCCTCCGTAA CGGGGATCAT GGCAGGATCC AACCGCTCCG GGGACCTCAA AGATGCCCAG 
AAGTCCATCC CAACAGGGAC CATTCTGGCC ATTGTGACTA CATCTTTCAT TTATCTTTCC 
TGCATTGTGC TGTTTGGGGC CTGCATCGAG GGTGTAGTCT TGCGAGATAA GTTTGGGGAG 
GCCCTGCAAG GGAACTTGGT CATTGGCATG CTGGCTTGGC CATCTCCCTG GGTCATCGTG 
ATTGGCTCCT TCTTCTCCAC CTGTGGTGCT GGCCTGCAGA GCCTAACTGG GGCACCCCGC 
CTGCTGCAGG CCATTGCACG CGACGGCATC ATCCCCTTCC TGCAGGTGTT TGGCCATGGA 
AAGGCCAACG GGGAGCCCAC GTGGGCCCTG CTGCTCACGG CCCTCATCTG TGAGACCGGC 
ATCCTCATCG CCTCCCTGGA CAGCGTGGCT CCCATCCTGT CCATGTTCTT CCTCATGTGC 
TACATGTTCG TCAACCTGGC CTGTGCTGTT CAGACCCTGC TGCGCACACC CAACTGGCGT 
CCACGTTTCA AGTTCTACCA CTGGACCCTC TCCTTCCTTG GGATGAGTCT CTGCCTTGCG 
CTCATGTTCA TCTGCTCCTG GTACTACGCC CTCTTCGCCA TGCTCATCGC GGGCTGCATC 
TACAAGTACA TCGAGTACCG AGGGGCTGAG AAGGAGTGGG GGGATGGCAT CAGGGGCCTG 
TCACTGAATG CTGCCCGCTA TGCCCTGCTG CGTGTGGAAC ATGGGCCCCC ACATACCAAG 
AACTGGAGGC CCCAGGTATT GGTGATGCTG AACCTGGACT CTGAGCAGTG TGTAAAGCAC 
CCCCGCCTGC TGTCCTTCAC CTCTCAGCTG AAGGCTGGCA AGGGCCTGAC CATCGTGGGA 
TCTGTGCTGG AGGGCACCTA CTTAGACAAG CATGTGGAGG CCCAGCGGGC TGAGGAGAAT 
ATCCGGTCTC TGATGAGTGC AGAGAAGATG AAAGGCTTCT GCCAGCTGGT GGTGTCGTCC 
AACCTGCGAG ACGGTGCATC CCACTTGATC CAGTCAGCTG GCCTTGGTGG CATGAAGCAC 
AACACTGTCC TCATGGCCTG GCCAGAGGCT TGGAAGCAGG CAGATAACCC TTTCTCCTGG 
AAGAACTTTG TGGACACTGT CCGTGACACT ACAGCAGCAC ATCAGGCACT GTTGGTGGCC 
AAGAACATCG ACTTGTTCCC GCAGAACCAG GAGCGCTTCA GCGACGGCAA CATCGATGTG 
TGGTGGATCG TGCACGATGG GGGGATGCTC ATGCTTCTGC CGTTCCTGCT GCGCCAGCAC 
AAGGTGTGGC GAAAGTGCCG GATGCGCATC TTCACGGTCG CCCAGGTGGA CGATAACAGC 
ATCCAGATGA AGAAGGACCT GCAGATGTTC CTGTACCATC TCAGGATCAG CGCCGAGGTG 
GAGGTGGTAG AGATGGTTGA AAATGACATT TCTGCATTCA CCTATGAGAA GACGCTAATG 
ATGGAGCAGA GGTCACAGAT GCTGAAACAG ATGCAGTTGT CAAAGAATGA GCGGGAGAGA 
GAGGCCCAGC TGATTCACGA CAGGAACACT GCATCCCACA CCGTAGCAAC GGCTAGAACT 
GAAGCCCCAC CAACACCTGA CAAAGTGCAG ATGACATGGA CGAAAGAGAA ACTCATTGCA 
GAGAAACACA GGAACAAGGA CACCGGCACA TCAGGCTTCA AAGACCTTTT CAGCCTAAAG 
CCGGACCAGT CCAACGTCAG AAGGATGCAT ACTGCTGTGA AGCTCAATGG TGTGGTTCTC 
AACAAGTCCC AGGATGCTCA ACTCGTCCTG CTGAATATGC CAGGCCCCCC AAAAAGTCGG 
CAGGGCGACG AGAACTACAT GGAATTTCTT GAGGTCTTGA CCGAAGGGCT GAACAGGGTC 
CTCCTGGTCA GGGGTGGTGG CCGGGAAGTC ATCACCATCT ACTCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

ZMPSTE24 defends against influenza and other pathogenic viruses.
The Journal of experimental medicine214(4)919-929(2017 Apr)
Fu B,Wang L,Li S,Dorf ME

The WNK-regulated SPAK/OSR1 kinases directly phosphorylate and inhibit the K+-Cl- co-transporters.
The Biochemical journal458(3)559-73(2014 Mar)
de Los Heros P,Alessi DR,Gourlay R,Campbell DG,Deak M,Macartney TJ,Kahle KT,Zhang J

Molecular evidence for a role for K(+)-Cl(-) cotransporters in the kidney.
American journal of physiology. Renal physiology305(10)F1402-11(2013 Nov)
Melo Z,Cruz-Rangel S,Bautista R,V?zquez N,Casta?eda-Bueno M,Mount DB,Pasantes-Morales H,Mercado A,Gamba G

Glycosylation regulates the function and membrane localization of KCC4.
Biochimica et biophysica acta1833(5)1133-46(2013 May)
Weng TY,Chiu WT,Liu HS,Cheng HC,Shen MR,Mount DB,Chou CY

Cell-type specific distribution of chloride transporters in the rat suprachiasmatic nucleus.
Neuroscience165(4)1519-37(2010 Feb)
Belenky MA,Sollars PJ,Mount DB,Alper SL,Yarom Y,Pickard GE

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Slc12a7 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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