Usb1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Usb1
Entrez Gene ID	307643
Full Name	U6 snRNA biogenesis phosphodiesterase 1
Synonyms	RGD1305215
General protein information	Preferred Names U6 snRNA biogenesis phosphodiesterase 1 Names U6 snRNA phosphodiesterase U6 snRNA biogenesis 1 UPF0406 protein C16orf57 homolog putative U6 snRNA phosphodiesterase
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 19 Map Location: 19p13

mRNA and Protein(s)

mRNA	Protein	Name
NM_001014013.1	NP_001014035.1	U6 snRNA phosphodiesterase

Pathways from BioSystems

Homologies

Oryza sativa (rice)	Os02g0651000	NP_001047590.1
Mus musculus (house mouse)	Usb1	NP_598715.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002220	XP_307963.4
Homo sapiens (human)	USB1	NP_078874.2
Pan troglodytes (chimpanzee)	USB1	XP_511000.1
Danio rerio (zebrafish)	usb1	NP_001003460.1
Canis lupus familiaris (dog)	USB1	XP_853879.2
Bos taurus (cattle)	USB1	NP_001068945.1
Rattus norvegicus (Norway rat)	Usb1	NP_001014035.1
Drosophila melanogaster (fruit fly)	CG16790	NP_649911.1
Xenopus tropicalis (tropical clawed frog)	usb1	NP_001120615.1
Gallus gallus (chicken)	C11H16ORF57	XP_413994.4
Arabidopsis thaliana (thale cress)	AT5G51170	NP_568753.1

Gene Ontology
Bibliography

Related articles in PubMed

Aberrant 3' oligoadenylation of spliceosomal U6 small nuclear RNA in poikiloderma with neutropenia.
Hilcenko C, Simpson PJ, Finch AJ, Bowler FR, Churcher MJ, Jin L, Packman LC, Shlien A, Campbell P, Kirwan M, Dokal I, Warren AJ
Blood121(6)1028-38(2013 Feb)

C16orf57, a gene mutated in poikiloderma with neutropenia, encodes a putative phosphodiesterase responsible for the U6 snRNA 3' end modification.
Mroczek S, Krwawicz J, Kutner J, Lazniewski M, Kuci?ski I, Ginalski K, Dziembowski A
Genes & development26(17)1911-25(2012 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Usb1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Usb1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa04589	NM_001014013.1 Latest version!	Rattus norvegicus U6 snRNA biogenesis phosphodiesterase 1 (Usb1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Usb1 ( NM_001014013.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001014035.1 Rattus norvegicus U6 snRNA biogenesis phosphodiesterase 1 (Usb1), mRNA.

Price & Availability↑

CloneID	ORa04589
Clone ID Related Accession (Same CDS sequence)	NM_001014013.1
Accession Version	NM_001014013.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 804bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-09-15
Organism	Rattus norvegicus(Norway rat)
Product	U6 snRNA phosphodiesterase
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC088280.1. On Apr 1, 2005 this sequence version replaced XM_226239.2. ##Evidence-Data-START## Transcript exon combination :: BC088280.1, EV772471.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01906347, SAMN01906353 [ECO:0000348] ##Evidence-Data-END##

ATGAACGCGG CGCCCCTGGT GGGCTACAGC AGCAGCGGTT CGGAGGATGA AGCGGAGGCC 
GTCGCCGCGG GGCGCAGCAA ACCGGGGTCT GGACTCCACC GTTGTGGCCA GAACCCACTT 
CCGAGTCAGA GGTTTCCAGT GCCCGACAGT GTGCTAAACA TGTTCCCCAG CACAGAGGAA 
GGACCTGAGG ATGACAGTGC AAGGCATGGG GGTCGCATCC GAACCTTTCC CCACGAGCGG 
GGCAACTGGG CCACCCACAT CTACATACCA TATGAGGCGA ATGAGGAGTT CCAGGATCTG 
CTTGACGTGT TGCTGCCCCG AGCTCAGATG TTCGCGCCTC GACTGGTGCA GATGGAGGAG 
TTCCACCTCA GCCTGTCTCA GAGCGTGGTT CTCCGTCACC ACTGGATCCT CCCCTTTGTG 
CAGGTGCTCA AAGACCGCAT GGCCTCTTTC CAAAGGTTCT TCTTTACTGC CAACAGGGTA 
AAGATTTATA CCAATCAAGA GAAAACCAGG ACCTTCATTG GGCTCGAGGT CAGCTCAGGG 
CATGCCCAGT TCCTGGACAT GGTTTCAGAG GTGGACAGAG TCATGAAGGA ATTTGACCTC 
ACTACTTTCT ACCAGGACCC TTCATTTCAT GTCAGTCTGG CCTGGTGTGT GGGTGATGCC 
CGTCTACAGT TGGAGGGACA GTGTCAGCAG GAACTACAGG AAATTGTGGA TGAGTTTGAA 
GACTCAGAGA TGCTGTTACG TGTGCTGGCT GAACAAGTCC GCTGCAAGTC CGGGAACAAA 
TTCTTCTCGA TGCCTTTGAA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001014035.1
CDS	29..832
Translation	MNAAPLVGYSSSGSEDEAEAVAAGRSKPGSGLHRCGQNPLPSQRFPVPDSVLNMFPSTEEGPEDDSARHGGRIRTFPHERGNWATHIYIPYEANEEFQDLLDVLLPRAQMFAPRLVQMEEFHLSLSQSVVLRHHWILPFVQVLKDRMASFQRFFFTANRVKIYTNQEKTRTFIGLEVSSGHAQFLDMVSEVDRVMKEFDLTTFYQDPSFHVSLAWCVGDARLQLEGQCQQELQEIVDEFEDSEMLLRVLAEQVRCKSGNKFFSMPLK

Target ORF information:

RefSeq Version	NM_001014013.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus U6 snRNA biogenesis phosphodiesterase 1 (Usb1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001014013.1

ORF Insert Sequence:

ATGAACGCGG CGCCCCTGGT GGGCTACAGC AGCAGCGGTT CGGAGGATGA AGCGGAGGCC 
GTCGCCGCGG GGCGCAGCAA ACCGGGGTCT GGACTCCACC GTTGTGGCCA GAACCCACTT 
CCGAGTCAGA GGTTTCCAGT GCCCGACAGT GTGCTAAACA TGTTCCCCAG CACAGAGGAA 
GGACCTGAGG ATGACAGTGC AAGGCATGGG GGTCGCATCC GAACCTTTCC CCACGAGCGG 
GGCAACTGGG CCACCCACAT CTACATACCA TATGAGGCGA ATGAGGAGTT CCAGGATCTG 
CTTGACGTGT TGCTGCCCCG AGCTCAGATG TTCGCGCCTC GACTGGTGCA GATGGAGGAG 
TTCCACCTCA GCCTGTCTCA GAGCGTGGTT CTCCGTCACC ACTGGATCCT CCCCTTTGTG 
CAGGTGCTCA AAGACCGCAT GGCCTCTTTC CAAAGGTTCT TCTTTACTGC CAACAGGGTA 
AAGATTTATA CCAATCAAGA GAAAACCAGG ACCTTCATTG GGCTCGAGGT CAGCTCAGGG 
CATGCCCAGT TCCTGGACAT GGTTTCAGAG GTGGACAGAG TCATGAAGGA ATTTGACCTC 
ACTACTTTCT ACCAGGACCC TTCATTTCAT GTCAGTCTGG CCTGGTGTGT GGGTGATGCC 
CGTCTACAGT TGGAGGGACA GTGTCAGCAG GAACTACAGG AAATTGTGGA TGAGTTTGAA 
GACTCAGAGA TGCTGTTACG TGTGCTGGCT GAACAAGTCC GCTGCAAGTC CGGGAACAAA 
TTCTTCTCGA TGCCTTTGAA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Aberrant 3' oligoadenylation of spliceosomal U6 small nuclear RNA in poikiloderma with neutropenia.
Blood121(6)1028-38(2013 Feb)
Hilcenko C,Simpson PJ,Finch AJ,Bowler FR,Churcher MJ,Jin L,Packman LC,Shlien A,Campbell P,Kirwan M,Dokal I,Warren AJ

C16orf57, a gene mutated in poikiloderma with neutropenia, encodes a putative phosphodiesterase responsible for the U6 snRNA 3' end modification.
Genes & development26(17)1911-25(2012 Sep)
Mroczek S,Krwawicz J,Kutner J,Lazniewski M,Kuci?ski I,Ginalski K,Dziembowski A