Cfap20 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Cfap20
Entrez Gene ID	307642
Full Name	cilia and flagella associated protein 20
Synonyms	Gtl3
General protein information	Preferred Names cilia and flagella associated protein 20 Names cilia- and flagella-associated protein 20 UPF0468 protein C16orf80 homolog gene trap locus 3 protein homolog
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 19 Map Location: 19p13

mRNA and Protein(s)

mRNA	Protein	Name
NM_001037978.1	NP_001033067.1	cilia- and flagella-associated protein 20
XM_006255083.1	XP_006255145.1	cilia- and flagella-associated protein 20 isoform X1

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Bug22 influences cilium morphology and the post-translational modification of ciliary microtubules.
Mendes Maia T, Gogendeau D, Pennetier C, Janke C, Basto R
Biology open3(2)138-51(2014 Feb)

The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts.
Baltz AG, Munschauer M, Schwanh?usser B, Vasile A, Murakawa Y, Schueler M, Youngs N, Penfold-Brown D, Drew K, Milek M, Wyler E, Bonneau R, Selbach M, Dieterich C, Landthaler M
Molecular cell46(5)674-90(2012 Jun)

Proteomic analysis of mammalian primary cilia.
Ishikawa H, Thompson J, Yates JR 3rd, Marshall WF
Current biology : CB22(5)414-9(2012 Mar)

Bug22p, a conserved centrosomal/ciliary protein also present in higher plants, is required for an effective ciliary stroke in Paramecium.
Lalign? C, Klotz C, de Loubresse NG, Lemullois M, Hori M, Laurent FX, Papon JF, Louis B, Cohen J, Koll F
Eukaryotic cell9(4)645-55(2010 Apr)

Large-scale proteomics and phosphoproteomics of urinary exosomes.
Gonzales PA, Pisitkun T, Hoffert JD, Tchapyjnikov D, Star RA, Kleta R, Wang NS, Knepper MA
Journal of the American Society of Nephrology : JASN20(2)363-79(2009 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Cfap20 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Cfap20 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa09572	NM_001037978.1 Latest version!	Rattus norvegicus cilia and flagella associated protein 20 (Cfap20), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$69.30 $99.00
ORa38592	XM_006255083.1 Latest version!	Rattus norvegicus cilia and flagella associated protein 20 (Cfap20), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Cfap20 ( NM_001037978.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001033067.1 Rattus norvegicus cilia and flagella associated protein 20 (Cfap20), mRNA.

Price & Availability↑

CloneID	ORa09572
Clone ID Related Accession (Same CDS sequence)	NM_001037978.1
Accession Version	NM_001037978.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 495bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-07
Organism	Rattus norvegicus(Norway rat)
Product	cilia- and flagella-associated protein 20
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC099768.1. On Dec 23, 2005 this sequence version replaced XM_226238.3. ##Evidence-Data-START## Transcript exon combination :: BC099768.1, DN936089.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052297, SAMN04563759 [ECO:0000348] ##Evidence-Data-END##

ATGTTCAAGA ACACGTTCCA GAGCGGCTTC CTCTCCATCC TCTACAGCAT CGGCAGCAAG 
CCCCTGCAGA TCTGGGACAA AAAGGTACGG AATGGCCACA TCAAAAGAAT CACTGACAAT 
GACATCCAGT CCCTGGTGCT AGAGATTGAA GGGACAAATG TCAGCACCAC ATACATCACG 
TGTCCTGCGG ACCCTAAGAA GACACTGGGG ATTAAACTGC CTTTCCTTGT CATGATCATC 
AAAAACTTGA AGAAGTATTT TACCTTCGAA GTGCAGGTAC TAGATGACAA GAACGTCCGT 
CGGAGGTTTC GAGCAAGTAA CTACCAGAGC ACCACTCGAG TCAAGCCCTT CATCTGTACC 
ATGCCCATGC GGCTGGATGA TGGCTGGAAC CAGATTCAGT TCAACCTATC TGACTTCACC 
CGGCGGGCAT ATGATCCATG CAAACTGTCG TATCCGAAGG GTTTACTTCT CAGACAGACT 
CTACTCAGAG GATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001033067.1
CDS	171..665
Translation	MFKNTFQSGFLSILYSIGSKPLQIWDKKVRNGHIKRITDNDIQSLVLEIEGTNVSTTYITCPADPKKTLGIKLPFLVMIIKNLKKYFTFEVQVLDDKNVRRRFRASNYQSTTRVKPFICTMPMRLDDGWNQIQFNLSDFTRRAYDPCKLSYPKGLLLRQTLLRG

Target ORF information:

RefSeq Version	NM_001037978.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus cilia and flagella associated protein 20 (Cfap20), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001037978.1

ORF Insert Sequence:

ATGTTCAAGA ACACGTTCCA GAGCGGCTTC CTCTCCATCC TCTACAGCAT CGGCAGCAAG 
CCCCTGCAGA TCTGGGACAA AAAGGTACGG AATGGCCACA TCAAAAGAAT CACTGACAAT 
GACATCCAGT CCCTGGTGCT AGAGATTGAA GGGACAAATG TCAGCACCAC ATACATCACG 
TGTCCTGCGG ACCCTAAGAA GACACTGGGG ATTAAACTGC CTTTCCTTGT CATGATCATC 
AAAAACTTGA AGAAGTATTT TACCTTCGAA GTGCAGGTAC TAGATGACAA GAACGTCCGT 
CGGAGGTTTC GAGCAAGTAA CTACCAGAGC ACCACTCGAG TCAAGCCCTT CATCTGTACC 
ATGCCCATGC GGCTGGATGA TGGCTGGAAC CAGATTCAGT TCAACCTATC TGACTTCACC 
CGGCGGGCAT ATGATCCATG CAAACTGTCG TATCCGAAGG GTTTACTTCT CAGACAGACT 
CTACTCAGAG GATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Cfap20 ( XM_006255083.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006255145.1 Rattus norvegicus cilia and flagella associated protein 20 (Cfap20), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa38592
Clone ID Related Accession (Same CDS sequence)	XM_006255083.1
Accession Version	XM_006255083.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 582bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	cilia- and flagella-associated protein 20 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005118.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCAAGA ACACGTTCCA GAGCGGCTTC CTCTCCATCC TCTACAGCAT CGGCAGCAAG 
CCCCTGCAGA TCTGGGACAA AAAGGTACGG AATGGCCACA TCAAAAGAAT CACTGACAAT 
GACATCCAGT CCCTGGTGCT AGAGATTGAA GGGACAAATG TCAGCACCAC ATACATCACG 
TGTCCTGCGG ACCCTAAGAA GACGCTGGGG ATTAAACTGC CTTTCCTTGT CATGATCATC 
AAAAACTTGA AGAAGTATTT TACCTTCGAA GTGCAGGTAC TAGATGACAA GAACGTCCGT 
CGGAGGTTTC GAGCAAGTAA CTACCAGAGC ACCACTCGAG TCAAGCCCTT CATCTGTACC 
ATGCCCATGC GGCTGGATGA TGGCTGGAAC CAGATTCAGT TCAACCTATC TGACTTCACC 
CGGCGGGCAT ATGGTACAAA CTACATCGAG ACCCTGAGAG TGCAGATCCA TGCAAACTGT 
CGTATCCGAA GGGTTTACTT CTCAGACAGA CTCTACTCAG AGGATGAGCT GCCAGCAGAG 
TTCAAACTAT ATCTACCAGT TCAGAACAAG GCCAAACAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006255145.1
CDS	257..838
Translation	MFKNTFQSGFLSILYSIGSKPLQIWDKKVRNGHIKRITDNDIQSLVLEIEGTNVSTTYITCPADPKKTLGIKLPFLVMIIKNLKKYFTFEVQVLDDKNVRRRFRASNYQSTTRVKPFICTMPMRLDDGWNQIQFNLSDFTRRAYGTNYIETLRVQIHANCRIRRVYFSDRLYSEDELPAEFKLYLPVQNKAKQ

Target ORF information:

RefSeq Version	XM_006255083.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus cilia and flagella associated protein 20 (Cfap20), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006255083.1

ORF Insert Sequence:

ATGTTCAAGA ACACGTTCCA GAGCGGCTTC CTCTCCATCC TCTACAGCAT CGGCAGCAAG 
CCCCTGCAGA TCTGGGACAA AAAGGTACGG AATGGCCACA TCAAAAGAAT CACTGACAAT 
GACATCCAGT CCCTGGTGCT AGAGATTGAA GGGACAAATG TCAGCACCAC ATACATCACG 
TGTCCTGCGG ACCCTAAGAA GACGCTGGGG ATTAAACTGC CTTTCCTTGT CATGATCATC 
AAAAACTTGA AGAAGTATTT TACCTTCGAA GTGCAGGTAC TAGATGACAA GAACGTCCGT 
CGGAGGTTTC GAGCAAGTAA CTACCAGAGC ACCACTCGAG TCAAGCCCTT CATCTGTACC 
ATGCCCATGC GGCTGGATGA TGGCTGGAAC CAGATTCAGT TCAACCTATC TGACTTCACC 
CGGCGGGCAT ATGGTACAAA CTACATCGAG ACCCTGAGAG TGCAGATCCA TGCAAACTGT 
CGTATCCGAA GGGTTTACTT CTCAGACAGA CTCTACTCAG AGGATGAGCT GCCAGCAGAG 
TTCAAACTAT ATCTACCAGT TCAGAACAAG GCCAAACAAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Bug22 influences cilium morphology and the post-translational modification of ciliary microtubules.
Biology open3(2)138-51(2014 Feb)
Mendes Maia T,Gogendeau D,Pennetier C,Janke C,Basto R

The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts.
Molecular cell46(5)674-90(2012 Jun)
Baltz AG,Munschauer M,Schwanh?usser B,Vasile A,Murakawa Y,Schueler M,Youngs N,Penfold-Brown D,Drew K,Milek M,Wyler E,Bonneau R,Selbach M,Dieterich C,Landthaler M

Proteomic analysis of mammalian primary cilia.
Current biology : CB22(5)414-9(2012 Mar)
Ishikawa H,Thompson J,Yates JR 3rd,Marshall WF

Bug22p, a conserved centrosomal/ciliary protein also present in higher plants, is required for an effective ciliary stroke in Paramecium.
Eukaryotic cell9(4)645-55(2010 Apr)
Lalign? C,Klotz C,de Loubresse NG,Lemullois M,Hori M,Laurent FX,Papon JF,Louis B,Cohen J,Koll F

Large-scale proteomics and phosphoproteomics of urinary exosomes.
Journal of the American Society of Nephrology : JASN20(2)363-79(2009 Feb)
Gonzales PA,Pisitkun T,Hoffert JD,Tchapyjnikov D,Star RA,Kleta R,Wang NS,Knepper MA

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Cfap20 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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