Spire1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Spire1
Entrez Gene ID	307348
Full Name	spire-type actin nucleation factor 1
General protein information	Preferred Names spire-type actin nucleation factor 1 Names protein spire homolog 1 spire homolog 1
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 18 Map Location: 18q12.1

mRNA and Protein(s)

mRNA	Protein	Name
XM_017600946.1	XP_017456435.1	protein spire homolog 1 isoform X2
NM_001107381.1	NP_001100851.1	protein spire homolog 1
XM_006254862.3	XP_006254924.1	protein spire homolog 1 isoform X3
XM_006254863.3	XP_006254925.1	protein spire homolog 1 isoform X4
XM_006254864.3	XP_006254926.1	protein spire homolog 1 isoform X5
XM_006254860.3	XP_006254922.1	protein spire homolog 1 isoform X1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	SPIRE1	XP_547684.4
Gallus gallus (chicken)	SPIRE1	XP_004939777.1
Mus musculus (house mouse)	Spire1	NP_919336.1
Xenopus tropicalis (tropical clawed frog)	spire1	XP_002936125.2
Pan troglodytes (chimpanzee)	SPIRE1	XP_003315842.1
Rattus norvegicus (Norway rat)	Spire1	NP_001100851.1
Danio rerio (zebrafish)	spire1a	NP_001038312.2
Homo sapiens (human)	SPIRE1	NP_001122098.1
Bos taurus (cattle)	SPIRE1	XP_005224371.1

Gene Ontology
Bibliography

Related articles in PubMed

DNA damage induces nuclear actin filament assembly by Formin -2 and Spire-? that promotes efficient DNA repair. [corrected].
Belin BJ, Lee T, Mullins RD
eLife4e07735(2015 Aug)

Enhanced fear expression in Spir-1 actin organizer mutant mice.
Pleiser S, Banchaabouchi MA, Samol-Wolf A, Farley D, Welz T, Wellbourne-Wood J, Gehring I, Linkner J, Faix J, Riemenschneider MJ, Dietrich S, Kerkhoff E
European journal of cell biology93(5-6)225-37(2014 May-Jun)

An actin-dependent mechanism for long-range vesicle transport.
Schuh M
Nature cell biology13(12)1431-6(2011 Oct)

Spire-type actin nucleators cooperate with Formin-2 to drive asymmetric oocyte division.
Pfender S, Kuznetsov V, Pleiser S, Kerkhoff E, Schuh M
Current biology : CB21(11)955-60(2011 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Spire1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Spire1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa05308	NM_001107381.1 Latest version!	Rattus norvegicus spire-type actin nucleation factor 1 (Spire1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$391.30 $559.00
ORa38459	XM_006254860.3 Latest version!	Rattus norvegicus spire-type actin nucleation factor 1 (Spire1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$391.30 $559.00
ORa38461	XM_006254862.3 Latest version!	Rattus norvegicus spire-type actin nucleation factor 1 (Spire1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$391.30 $559.00
ORa38462	XM_006254863.3 Latest version!	Rattus norvegicus spire-type actin nucleation factor 1 (Spire1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$391.30 $559.00
ORa38463	XM_006254864.3 Latest version!	Rattus norvegicus spire-type actin nucleation factor 1 (Spire1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$391.30 $559.00
ORa59261	XM_017600946.1 Latest version!	Rattus norvegicus spire-type actin nucleation factor 1 (Spire1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$391.30 $559.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Spire1 ( NM_001107381.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001100851.1 Rattus norvegicus spire-type actin nucleation factor 1 (Spire1), mRNA.

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CloneID	ORa05308
Clone ID Related Accession (Same CDS sequence)	NM_001107381.1
Accession Version	NM_001107381.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2250bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-24
Organism	Rattus norvegicus(Norway rat)
Product	protein spire homolog 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CH473971.1. On or before Oct 4, 2007 this sequence version replaced XM_225864.4, XM_001061383.1. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGGCGCAGC CCTCAAGCCC GGGCGGCGAG GGGCCGCAGC TCGGCGCGAC AGGGGACCCG 
CGCGACGCGC TGAGCTTGGA AGAGATCCTG CGGCTCTACA ACCAGCCCAT CAACGAGGAG 
CAGGCGTGGG CCGTGTGCTT CCAGTGCTGC GGCTCCCTGC GCGCCGCCGC CCGCCGCCAG 
CCCCACCGCC GCGTGCGCTC GGCCGCGCAG ATCCGCGTTA TCGAATCCCT GGGAATTATT 
ATTTATAAAG CACTGGACTA TGGCTTGAAG GAAAATGAAG AGAGGGAGCT GAGCCCTCCT 
CTCGAGCAGC TCATCGACCA AATGGCCAAT ACCTCGGAGG CGGATGGAAG CAATGATGAA 
GGGTACGAGG CAGCAGATGA AGGCCCAGAC GAGGAAGATG GAGAGAGGAG AAGCAGCGCA 
GCCATTCGGT CCTATCAAGA TGTCATGAAG ATATGTGCTG CTCATCTCCC CACTGAGTCG 
GAGGCACCGA ATCATTATCA GGCAGTATGT CGGGCACTGT TTGCAGAAAC CATGGAACTG 
CATACGTTTC TGACCAAAAT TAAGAGTGCG AAGGAGAACC TTAAGAAGAT TCAAGAAATG 
GAAAAGAGTG ATGACTGTAG CACAGATCTG GAGGACCTGA AAAATGCAGA CTGGGCACGG 
TTCTGGGTAC AGGTGATGAG GGATTTGCGA AATGGGGTAA AACTTAAGAA AGTCCAACAG 
CGGCAGTACA ACCCATTGCC CATTGAATAC CAGCTCACCC CGTATGAGAT GCTCATGGAC 
GACATTCGGT GCAAAAGATA CACCTTGAGA AAAGTAATGG TAAATGGTGA CATTCCCCCT 
CGGTTAAGAA AGAGTGCTCA TGAGATCATC CTTGACTTCA TCAGATCAAG ACCCCCTTTA 
AATCCAGCTT CAGCCAGAAA ACTGAAACCA ACCCCACCAC GGCCACGGAG CCTCCATGAA 
AGAATACTAG AAGAAATTAA AGCAGAAAGA AAGCTTCGGC CTGTCTCACC AGAAGAAATT 
AGACGGAGCA GACTAGATGT TACTACACCA GAATCTCCAA AGAATGTGGG GAAATCATCT 
GTGGTGAATG GAGACTTAAC ATCTCAAACG AAAGAAAATG GGCTGGGTGC TGCCCAGCCG 
GGGCCTGCAC AGCCAAAGAG GCTCCTCAAA GCCCCGACGC TGGCTGAGCT GGACAGCTCT 
GACTCTGAGG AGGAAACTCT GCACAAGTCA ACCAGCAGCA GCAGTGCATC TCCCTCCCTC 
TATGAGGACC CAGTACTGGA GGCCATGTGC TCCAAGAAGA AGCCTCCAAA ATTCTTGCCC 
ATCTCATCAA CACCCCAGCC CGAGAGAAGG CAACCACCAC AGAGGCGCCA CTCCATTGAG 
AAGGAAACTC CTACAAATGT GAGGCAGTTT CTGCCCCCAT CCAGGCAGAG CTCACGTTCT 
CTTGTTCCTA GGATAACCAG TGTATGGCCA AGGACGCCTT TCCGACCACT TTTCTCTGCC 
ATACAAACAG CTTCTCTGCT GAGCTCTCAT CCTTTTGAAG CAGCCATGTT TGGGGTAGCA 
GGGGCTATGT ATTATCTGTT TGAGAGAGCT TTTACCAGCA GGTGGAAACC CTCAAAGGAG 
GAATTCTGCT ACCCAGTGGA ATGCCTCGCT CTCACTGTGG AGGAAGTGAT GCACATTCGT 
CAGGTCCTGG TGAAAGCGGA GCTGGAAAAG TACCAACAGT ATAAGGATGT CTACACTGCC 
CTGAAAAAAG GAAAGCTCTG CTTCTGTTGC CGAACCCGGA GGTTTTCCTT CTTCACCTGG 
TCCTACACCT GTCAGTTCTG TAAGAGGCCC GTGTGCTCAC AGTGTTGCAA AAAGATGCGG 
CTGCCTTCGA AGCCATACTC CACTTTACCT ATCTTCTCTT TGGGACCTTC TGCCTTGCAA 
AGAGGGGAAA GTTGTTCGAG GTCAGAAAAA CCTTCTACTG GTCACCATCG GCCGCTTCGA 
AGTATTACCA GATTCTCTTC AAAATCAAAG TCTGTGGACA AATCAGATGA AGAACTCCAG 
TTTCCCAAAG AGTTCATGGA AGATTGGAGC ACAATGGAGG TGTGTGTAGA CTGCAAAAAG 
TTCATTTCAG AGATCATCAG CTCAAGCCGG CGCAGCCTGG TGTTGGCCAA CAAAAGAGCC 
AGGTTGAAAA GGAAGACACA GTCTTTCTAC ATGTCCTCAG CGGGCCCCTC GGAGTACTGC 
CCTTCAGAAC GGACTATCAA CGAGATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001100851.1
CDS	89..2338
Translation	MAQPSSPGGEGPQLGATGDPRDALSLEEILRLYNQPINEEQAWAVCFQCCGSLRAAARRQPHRRVRSAAQIRVIESLGIIIYKALDYGLKENEERELSPPLEQLIDQMANTSEADGSNDEGYEAADEGPDEEDGERRSSAAIRSYQDVMKICAAHLPTESEAPNHYQAVCRALFAETMELHTFLTKIKSAKENLKKIQEMEKSDDCSTDLEDLKNADWARFWVQVMRDLRNGVKLKKVQQRQYNPLPIEYQLTPYEMLMDDIRCKRYTLRKVMVNGDIPPRLRKSAHEIILDFIRSRPPLNPASARKLKPTPPRPRSLHERILEEIKAERKLRPVSPEEIRRSRLDVTTPESPKNVGKSSVVNGDLTSQTKENGLGAAQPGPAQPKRLLKAPTLAELDSSDSEEETLHKSTSSSSASPSLYEDPVLEAMCSKKKPPKFLPISSTPQPERRQPPQRRHSIEKETPTNVRQFLPPSRQSSRSLVPRITSVWPRTPFRPLFSAIQTASLLSSHPFEAAMFGVAGAMYYLFERAFTSRWKPSKEEFCYPVECLALTVEEVMHIRQVLVKAELEKYQQYKDVYTALKKGKLCFCCRTRRFSFFTWSYTCQFCKRPVCSQCCKKMRLPSKPYSTLPIFSLGPSALQRGESCSRSEKPSTGHHRPLRSITRFSSKSKSVDKSDEELQFPKEFMEDWSTMEVCVDCKKFISEIISSSRRSLVLANKRARLKRKTQSFYMSSAGPSEYCPSERTINEI

Target ORF information:

RefSeq Version	NM_001107381.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus spire-type actin nucleation factor 1 (Spire1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001107381.1

ORF Insert Sequence:

ATGGCGCAGC CCTCAAGCCC GGGCGGCGAG GGGCCGCAGC TCGGCGCGAC AGGGGACCCG 
CGCGACGCGC TGAGCTTGGA AGAGATCCTG CGGCTCTACA ACCAGCCCAT CAACGAGGAG 
CAGGCGTGGG CCGTGTGCTT CCAGTGCTGC GGCTCCCTGC GCGCCGCCGC CCGCCGCCAG 
CCCCACCGCC GCGTGCGCTC GGCCGCGCAG ATCCGCGTTA TCGAATCCCT GGGAATTATT 
ATTTATAAAG CACTGGACTA TGGCTTGAAG GAAAATGAAG AGAGGGAGCT GAGCCCTCCT 
CTCGAGCAGC TCATCGACCA AATGGCCAAT ACCTCGGAGG CGGATGGAAG CAATGATGAA 
GGGTACGAGG CAGCAGATGA AGGCCCAGAC GAGGAAGATG GAGAGAGGAG AAGCAGCGCA 
GCCATTCGGT CCTATCAAGA TGTCATGAAG ATATGTGCTG CTCATCTCCC CACTGAGTCG 
GAGGCACCGA ATCATTATCA GGCAGTATGT CGGGCACTGT TTGCAGAAAC CATGGAACTG 
CATACGTTTC TGACCAAAAT TAAGAGTGCG AAGGAGAACC TTAAGAAGAT TCAAGAAATG 
GAAAAGAGTG ATGACTGTAG CACAGATCTG GAGGACCTGA AAAATGCAGA CTGGGCACGG 
TTCTGGGTAC AGGTGATGAG GGATTTGCGA AATGGGGTAA AACTTAAGAA AGTCCAACAG 
CGGCAGTACA ACCCATTGCC CATTGAATAC CAGCTCACCC CGTATGAGAT GCTCATGGAC 
GACATTCGGT GCAAAAGATA CACCTTGAGA AAAGTAATGG TAAATGGTGA CATTCCCCCT 
CGGTTAAGAA AGAGTGCTCA TGAGATCATC CTTGACTTCA TCAGATCAAG ACCCCCTTTA 
AATCCAGCTT CAGCCAGAAA ACTGAAACCA ACCCCACCAC GGCCACGGAG CCTCCATGAA 
AGAATACTAG AAGAAATTAA AGCAGAAAGA AAGCTTCGGC CTGTCTCACC AGAAGAAATT 
AGACGGAGCA GACTAGATGT TACTACACCA GAATCTCCAA AGAATGTGGG GAAATCATCT 
GTGGTGAATG GAGACTTAAC ATCTCAAACG AAAGAAAATG GGCTGGGTGC TGCCCAGCCG 
GGGCCTGCAC AGCCAAAGAG GCTCCTCAAA GCCCCGACGC TGGCTGAGCT GGACAGCTCT 
GACTCTGAGG AGGAAACTCT GCACAAGTCA ACCAGCAGCA GCAGTGCATC TCCCTCCCTC 
TATGAGGACC CAGTACTGGA GGCCATGTGC TCCAAGAAGA AGCCTCCAAA ATTCTTGCCC 
ATCTCATCAA CACCCCAGCC CGAGAGAAGG CAACCACCAC AGAGGCGCCA CTCCATTGAG 
AAGGAAACTC CTACAAATGT GAGGCAGTTT CTGCCCCCAT CCAGGCAGAG CTCACGTTCT 
CTTGTTCCTA GGATAACCAG TGTATGGCCA AGGACGCCTT TCCGACCACT TTTCTCTGCC 
ATACAAACAG CTTCTCTGCT GAGCTCTCAT CCTTTTGAAG CAGCCATGTT TGGGGTAGCA 
GGGGCTATGT ATTATCTGTT TGAGAGAGCT TTTACCAGCA GGTGGAAACC CTCAAAGGAG 
GAATTCTGCT ACCCAGTGGA ATGCCTCGCT CTCACTGTGG AGGAAGTGAT GCACATTCGT 
CAGGTCCTGG TGAAAGCGGA GCTGGAAAAG TACCAACAGT ATAAGGATGT CTACACTGCC 
CTGAAAAAAG GAAAGCTCTG CTTCTGTTGC CGAACCCGGA GGTTTTCCTT CTTCACCTGG 
TCCTACACCT GTCAGTTCTG TAAGAGGCCC GTGTGCTCAC AGTGTTGCAA AAAGATGCGG 
CTGCCTTCGA AGCCATACTC CACTTTACCT ATCTTCTCTT TGGGACCTTC TGCCTTGCAA 
AGAGGGGAAA GTTGTTCGAG GTCAGAAAAA CCTTCTACTG GTCACCATCG GCCGCTTCGA 
AGTATTACCA GATTCTCTTC AAAATCAAAG TCTGTGGACA AATCAGATGA AGAACTCCAG 
TTTCCCAAAG AGTTCATGGA AGATTGGAGC ACAATGGAGG TGTGTGTAGA CTGCAAAAAG 
TTCATTTCAG AGATCATCAG CTCAAGCCGG CGCAGCCTGG TGTTGGCCAA CAAAAGAGCC 
AGGTTGAAAA GGAAGACACA GTCTTTCTAC ATGTCCTCAG CGGGCCCCTC GGAGTACTGC 
CCTTCAGAAC GGACTATCAA CGAGATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Spire1 ( XM_006254860.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006254922.1 Rattus norvegicus spire-type actin nucleation factor 1 (Spire1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa38459
Clone ID Related Accession (Same CDS sequence)	XM_006254860.3
Accession Version	XM_006254860.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2397bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	protein spire homolog 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005117.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006254860.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCAGC CCTCAAGCCC GGGCGGCGAG GGGCCGCAGC TCGGCGCGAC AGGGGACCCG 
CGCGACGCGC TGAGCTTGGA AGAGATCCTG CGGCTCTACA ACCAGCCCAT CAACGAGGAG 
CAGGCGTGGG CCGTGTGCTT CCAGTGCTGC GGCTCCCTGC GCGCCGCCGC CCGCCGCCAG 
CCCCACCGCC GCGTGCGCTC GGCCGCGCAG ATCCGCGTCT GGAGGGACGG CGCCGTCACC 
CTGGCGCCCG CCGCGGACGA GGAGGGAGAG CCGCCCCCTG CCTCCGGTAA ATTGGGATAT 
TCACATTGTA CAGAAACAGA GGTTATCGAA TCCCTGGGAA TTATTATTTA TAAAGCACTG 
GACTATGGCT TGAAGGAAAA TGAAGAGAGG GAGCTGAGCC CTCCTCTCGA GCAGCTCATC 
GACCAAATGG CCAATACCTC GGAGGCGGAT GGAAGCAATG ATGAAGGGTA CGAGGCAGCA 
GATGAAGGCC CAGACGAGGA AGATGGAGAG AGGAGAAGCA GCGCAGCCAT TCGGTCCTAT 
CAAGATGTCA TGAAGATATG TGCTGCTCAT CTCCCCACTG AGTCGGAGGC ACCGAATCAT 
TATCAGGCAG TATGTCGGGC ACTGTTTGCA GAAACCATGG AACTGCATAC GTTTCTGACC 
AAAATTAAGA GTGCGAAGGA GAACCTTAAG AAGATTCAAG AAATGGAAAA GAGTGATGAC 
TGTAGCACAG ATCTGGAGGA CCTGAAAAAT GCAGACTGGG CACGGTTCTG GGTACAGGTG 
ATGAGGGATT TGCGAAATGG GGTAAAACTT AAGAAAGTCC AACAGCGGCA GTACAACCCA 
TTGCCCATTG AATACCAGCT CACCCCGTAT GAGATGCTCA TGGACGACAT TCGGTGCAAA 
AGATACACCT TGAGAAAAGT AATGGTAAAT GGTGACATTC CCCCTCGGTT AAGAAAGAGT 
GCTCATGAGA TCATCCTTGA CTTCATCAGA TCAAGACCCC CTTTAAATCC AGCTTCAGCC 
AGAAAACTGA AACCAACCCC ACCACGGCCA CGGAGCCTCC ATGAAAGAAT ACTAGAAGAA 
ATTAAAGCAG AAAGAAAGCT TCGGCCTGTC TCACCAGAAG AAATTAGACG GAGCAGACTA 
GCAGTTCGGC CACTTAGCAT GTCTCACAGT TTTGACTTGT CAGATGTTAC TACACCAGAA 
TCTCCAAAGA ATGTGGGGAA ATCATCTGTG GTGAATGGAG ACTTAACATC TCAAACGAAA 
GAAAATGGGC TGGGTGCTGC CCAGCCGGGG CCTGCACAGC CAAAGAGGCT CCTCAAAGCC 
CCGACGCTGG CTGAGCTGGA CAGCTCTGAC TCTGAGGAGG AAACTCTGCA CAAGTCAACC 
AGCAGCAGCA GTGCATCTCC CTCCCTCTAT GAGGACCCAG TACTGGAGGC CATGTGCTCC 
AAGAAGAAGC CTCCAAAATT CTTGCCCATC TCATCAACAC CCCAGCCCGA GAGAAGGCAA 
CCACCACAGA GGCGCCACTC CATTGAGAAG GAAACTCCTA CAAATGTGAG GCAGTTTCTG 
CCCCCATCCA GGCAGAGCTC ACGTTCTCTT GTTCCTAGGA TAACCAGTGT ATGGCCAAGG 
ACGCCTTTCC GACCACTTTT CTCTGCCATA CAAACAGCTT CTCTGCTGAG CTCTCATCCT 
TTTGAAGCAG CCATGTTTGG GGTAGCAGGG GCTATGTATT ATCTGTTTGA GAGAGCTTTT 
ACCAGCAGGT GGAAACCCTC AAAGGAGGAA TTCTGCTACC CAGTGGAATG CCTCGCTCTC 
ACTGTGGAGG AAGTGATGCA CATTCGTCAG GTCCTGGTGA AAGCGGAGCT GGAAAAGTAC 
CAACAGTATA AGGATGTCTA CACTGCCCTG AAAAAAGGAA AGCTCTGCTT CTGTTGCCGA 
ACCCGGAGGT TTTCCTTCTT CACCTGGTCC TACACCTGTC AGTTCTGTAA GAGGCCCGTG 
TGCTCACAGT GTTGCAAAAA GATGCGGCTG CCTTCGAAGC CATACTCCAC TTTACCTATC 
TTCTCTTTGG GACCTTCTGC CTTGCAAAGA GGGGAAAGTT GTTCGAGGTC AGAAAAACCT 
TCTACTGGTC ACCATCGGCC GCTTCGAAGT ATTACCAGAT TCTCTTCAAA ATCAAAGTCT 
GTGGACAAAT CAGATGAAGA ACTCCAGTTT CCCAAAGAGT TCATGGAAGA TTGGAGCACA 
ATGGAGGTGT GTGTAGACTG CAAAAAGTTC ATTTCAGAGA TCATCAGCTC AAGCCGGCGC 
AGCCTGGTGT TGGCCAACAA AAGAGCCAGG TTGAAAAGGA AGACACAGTC TTTCTACATG 
TCCTCAGCGG GCCCCTCGGA GTACTGCCCT TCAGAACGGA CTATCAACGA GATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006254922.1
CDS	211..2607
Translation	MAQPSSPGGEGPQLGATGDPRDALSLEEILRLYNQPINEEQAWAVCFQCCGSLRAAARRQPHRRVRSAAQIRVWRDGAVTLAPAADEEGEPPPASGKLGYSHCTETEVIESLGIIIYKALDYGLKENEERELSPPLEQLIDQMANTSEADGSNDEGYEAADEGPDEEDGERRSSAAIRSYQDVMKICAAHLPTESEAPNHYQAVCRALFAETMELHTFLTKIKSAKENLKKIQEMEKSDDCSTDLEDLKNADWARFWVQVMRDLRNGVKLKKVQQRQYNPLPIEYQLTPYEMLMDDIRCKRYTLRKVMVNGDIPPRLRKSAHEIILDFIRSRPPLNPASARKLKPTPPRPRSLHERILEEIKAERKLRPVSPEEIRRSRLAVRPLSMSHSFDLSDVTTPESPKNVGKSSVVNGDLTSQTKENGLGAAQPGPAQPKRLLKAPTLAELDSSDSEEETLHKSTSSSSASPSLYEDPVLEAMCSKKKPPKFLPISSTPQPERRQPPQRRHSIEKETPTNVRQFLPPSRQSSRSLVPRITSVWPRTPFRPLFSAIQTASLLSSHPFEAAMFGVAGAMYYLFERAFTSRWKPSKEEFCYPVECLALTVEEVMHIRQVLVKAELEKYQQYKDVYTALKKGKLCFCCRTRRFSFFTWSYTCQFCKRPVCSQCCKKMRLPSKPYSTLPIFSLGPSALQRGESCSRSEKPSTGHHRPLRSITRFSSKSKSVDKSDEELQFPKEFMEDWSTMEVCVDCKKFISEIISSSRRSLVLANKRARLKRKTQSFYMSSAGPSEYCPSERTINEI

Target ORF information:

RefSeq Version	XM_006254860.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus spire-type actin nucleation factor 1 (Spire1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006254860.3

ORF Insert Sequence:

ATGGCGCAGC CCTCAAGCCC GGGCGGCGAG GGGCCGCAGC TCGGCGCGAC AGGGGACCCG 
CGCGACGCGC TGAGCTTGGA AGAGATCCTG CGGCTCTACA ACCAGCCCAT CAACGAGGAG 
CAGGCGTGGG CCGTGTGCTT CCAGTGCTGC GGCTCCCTGC GCGCCGCCGC CCGCCGCCAG 
CCCCACCGCC GCGTGCGCTC GGCCGCGCAG ATCCGCGTCT GGAGGGACGG CGCCGTCACC 
CTGGCGCCCG CCGCGGACGA GGAGGGAGAG CCGCCCCCTG CCTCCGGTAA ATTGGGATAT 
TCACATTGTA CAGAAACAGA GGTTATCGAA TCCCTGGGAA TTATTATTTA TAAAGCACTG 
GACTATGGCT TGAAGGAAAA TGAAGAGAGG GAGCTGAGCC CTCCTCTCGA GCAGCTCATC 
GACCAAATGG CCAATACCTC GGAGGCGGAT GGAAGCAATG ATGAAGGGTA CGAGGCAGCA 
GATGAAGGCC CAGACGAGGA AGATGGAGAG AGGAGAAGCA GCGCAGCCAT TCGGTCCTAT 
CAAGATGTCA TGAAGATATG TGCTGCTCAT CTCCCCACTG AGTCGGAGGC ACCGAATCAT 
TATCAGGCAG TATGTCGGGC ACTGTTTGCA GAAACCATGG AACTGCATAC GTTTCTGACC 
AAAATTAAGA GTGCGAAGGA GAACCTTAAG AAGATTCAAG AAATGGAAAA GAGTGATGAC 
TGTAGCACAG ATCTGGAGGA CCTGAAAAAT GCAGACTGGG CACGGTTCTG GGTACAGGTG 
ATGAGGGATT TGCGAAATGG GGTAAAACTT AAGAAAGTCC AACAGCGGCA GTACAACCCA 
TTGCCCATTG AATACCAGCT CACCCCGTAT GAGATGCTCA TGGACGACAT TCGGTGCAAA 
AGATACACCT TGAGAAAAGT AATGGTAAAT GGTGACATTC CCCCTCGGTT AAGAAAGAGT 
GCTCATGAGA TCATCCTTGA CTTCATCAGA TCAAGACCCC CTTTAAATCC AGCTTCAGCC 
AGAAAACTGA AACCAACCCC ACCACGGCCA CGGAGCCTCC ATGAAAGAAT ACTAGAAGAA 
ATTAAAGCAG AAAGAAAGCT TCGGCCTGTC TCACCAGAAG AAATTAGACG GAGCAGACTA 
GCAGTTCGGC CACTTAGCAT GTCTCACAGT TTTGACTTGT CAGATGTTAC TACACCAGAA 
TCTCCAAAGA ATGTGGGGAA ATCATCTGTG GTGAATGGAG ACTTAACATC TCAAACGAAA 
GAAAATGGGC TGGGTGCTGC CCAGCCGGGG CCTGCACAGC CAAAGAGGCT CCTCAAAGCC 
CCGACGCTGG CTGAGCTGGA CAGCTCTGAC TCTGAGGAGG AAACTCTGCA CAAGTCAACC 
AGCAGCAGCA GTGCATCTCC CTCCCTCTAT GAGGACCCAG TACTGGAGGC CATGTGCTCC 
AAGAAGAAGC CTCCAAAATT CTTGCCCATC TCATCAACAC CCCAGCCCGA GAGAAGGCAA 
CCACCACAGA GGCGCCACTC CATTGAGAAG GAAACTCCTA CAAATGTGAG GCAGTTTCTG 
CCCCCATCCA GGCAGAGCTC ACGTTCTCTT GTTCCTAGGA TAACCAGTGT ATGGCCAAGG 
ACGCCTTTCC GACCACTTTT CTCTGCCATA CAAACAGCTT CTCTGCTGAG CTCTCATCCT 
TTTGAAGCAG CCATGTTTGG GGTAGCAGGG GCTATGTATT ATCTGTTTGA GAGAGCTTTT 
ACCAGCAGGT GGAAACCCTC AAAGGAGGAA TTCTGCTACC CAGTGGAATG CCTCGCTCTC 
ACTGTGGAGG AAGTGATGCA CATTCGTCAG GTCCTGGTGA AAGCGGAGCT GGAAAAGTAC 
CAACAGTATA AGGATGTCTA CACTGCCCTG AAAAAAGGAA AGCTCTGCTT CTGTTGCCGA 
ACCCGGAGGT TTTCCTTCTT CACCTGGTCC TACACCTGTC AGTTCTGTAA GAGGCCCGTG 
TGCTCACAGT GTTGCAAAAA GATGCGGCTG CCTTCGAAGC CATACTCCAC TTTACCTATC 
TTCTCTTTGG GACCTTCTGC CTTGCAAAGA GGGGAAAGTT GTTCGAGGTC AGAAAAACCT 
TCTACTGGTC ACCATCGGCC GCTTCGAAGT ATTACCAGAT TCTCTTCAAA ATCAAAGTCT 
GTGGACAAAT CAGATGAAGA ACTCCAGTTT CCCAAAGAGT TCATGGAAGA TTGGAGCACA 
ATGGAGGTGT GTGTAGACTG CAAAAAGTTC ATTTCAGAGA TCATCAGCTC AAGCCGGCGC 
AGCCTGGTGT TGGCCAACAA AAGAGCCAGG TTGAAAAGGA AGACACAGTC TTTCTACATG 
TCCTCAGCGG GCCCCTCGGA GTACTGCCCT TCAGAACGGA CTATCAACGA GATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Spire1 ( XM_006254862.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006254924.1 Rattus norvegicus spire-type actin nucleation factor 1 (Spire1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ORa38461
Clone ID Related Accession (Same CDS sequence)	XM_006254862.3
Accession Version	XM_006254862.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2355bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	protein spire homolog 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005117.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006254862.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCAGC CCTCAAGCCC GGGCGGCGAG GGGCCGCAGC TCGGCGCGAC AGGGGACCCG 
CGCGACGCGC TGAGCTTGGA AGAGATCCTG CGGCTCTACA ACCAGCCCAT CAACGAGGAG 
CAGGCGTGGG CCGTGTGCTT CCAGTGCTGC GGCTCCCTGC GCGCCGCCGC CCGCCGCCAG 
CCCCACCGCC GCGTGCGCTC GGCCGCGCAG ATCCGCGTCT GGAGGGACGG CGCCGTCACC 
CTGGCGCCCG CCGCGGACGA GGAGGGAGAG CCGCCCCCTG CCTCCGGTAA ATTGGGATAT 
TCACATTGTA CAGAAACAGA GGTTATCGAA TCCCTGGGAA TTATTATTTA TAAAGCACTG 
GACTATGGCT TGAAGGAAAA TGAAGAGAGG GAGCTGAGCC CTCCTCTCGA GCAGCTCATC 
GACCAAATGG CCAATACCTC GGAGGCGGAT GGAAGCAATG ATGAAGGGTA CGAGGCAGCA 
GATGAAGGCC CAGACGAGGA AGATGGAGAG AGGAGAAGCA GCGCAGCCAT TCGGTCCTAT 
CAAGATGTCA TGAAGATATG TGCTGCTCAT CTCCCCACTG AGTCGGAGGC ACCGAATCAT 
TATCAGGCAG TATGTCGGGC ACTGTTTGCA GAAACCATGG AACTGCATAC GTTTCTGACC 
AAAATTAAGA GTGCGAAGGA GAACCTTAAG AAGATTCAAG AAATGGAAAA GAGTGATGAC 
TGTAGCACAG ATCTGGAGGA CCTGAAAAAT GCAGACTGGG CACGGTTCTG GGTACAGGTG 
ATGAGGGATT TGCGAAATGG GGTAAAACTT AAGAAAGTCC AACAGCGGCA GTACAACCCA 
TTGCCCATTG AATACCAGCT CACCCCGTAT GAGATGCTCA TGGACGACAT TCGGTGCAAA 
AGATACACCT TGAGAAAAGT AATGGTAAAT GGTGACATTC CCCCTCGGTT AAGAAAGAGT 
GCTCATGAGA TCATCCTTGA CTTCATCAGA TCAAGACCCC CTTTAAATCC AGCTTCAGCC 
AGAAAACTGA AACCAACCCC ACCACGGCCA CGGAGCCTCC ATGAAAGAAT ACTAGAAGAA 
ATTAAAGCAG AAAGAAAGCT TCGGCCTGTC TCACCAGAAG AAATTAGACG GAGCAGACTA 
GATGTTACTA CACCAGAATC TCCAAAGAAT GTGGGGAAAT CATCTGTGGT GAATGGAGAC 
TTAACATCTC AAACGAAAGA AAATGGGCTG GGTGCTGCCC AGCCGGGGCC TGCACAGCCA 
AAGAGGCTCC TCAAAGCCCC GACGCTGGCT GAGCTGGACA GCTCTGACTC TGAGGAGGAA 
ACTCTGCACA AGTCAACCAG CAGCAGCAGT GCATCTCCCT CCCTCTATGA GGACCCAGTA 
CTGGAGGCCA TGTGCTCCAA GAAGAAGCCT CCAAAATTCT TGCCCATCTC ATCAACACCC 
CAGCCCGAGA GAAGGCAACC ACCACAGAGG CGCCACTCCA TTGAGAAGGA AACTCCTACA 
AATGTGAGGC AGTTTCTGCC CCCATCCAGG CAGAGCTCAC GTTCTCTTGT TCCTAGGATA 
ACCAGTGTAT GGCCAAGGAC GCCTTTCCGA CCACTTTTCT CTGCCATACA AACAGCTTCT 
CTGCTGAGCT CTCATCCTTT TGAAGCAGCC ATGTTTGGGG TAGCAGGGGC TATGTATTAT 
CTGTTTGAGA GAGCTTTTAC CAGCAGGTGG AAACCCTCAA AGGAGGAATT CTGCTACCCA 
GTGGAATGCC TCGCTCTCAC TGTGGAGGAA GTGATGCACA TTCGTCAGGT CCTGGTGAAA 
GCGGAGCTGG AAAAGTACCA ACAGTATAAG GATGTCTACA CTGCCCTGAA AAAAGGAAAG 
CTCTGCTTCT GTTGCCGAAC CCGGAGGTTT TCCTTCTTCA CCTGGTCCTA CACCTGTCAG 
TTCTGTAAGA GGCCCGTGTG CTCACAGTGT TGCAAAAAGA TGCGGCTGCC TTCGAAGCCA 
TACTCCACTT TACCTATCTT CTCTTTGGGA CCTTCTGCCT TGCAAAGAGG GGAAAGTTGT 
TCGAGGTCAG AAAAACCTTC TACTGGTCAC CATCGGCCGC TTCGAAGTAT TACCAGATTC 
TCTTCAAAAT CAAAGTCTGT GGACAAATCA GATGAAGAAC TCCAGTTTCC CAAAGAGTTC 
ATGGAAGATT GGAGCACAAT GGAGGTGTGT GTAGACTGCA AAAAGTTCAT TTCAGAGATC 
ATCAGCTCAA GCCGGCGCAG CCTGGTGTTG GCCAACAAAA GAGCCAGGTT GAAAAGGAAG 
ACACAGTCTT TCTACATGTC CTCAGCGGGC CCCTCGGAGT ACTGCCCTTC AGAACGGACT 
ATCAACGAGA TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006254924.1
CDS	210..2564
Translation	MAQPSSPGGEGPQLGATGDPRDALSLEEILRLYNQPINEEQAWAVCFQCCGSLRAAARRQPHRRVRSAAQIRVWRDGAVTLAPAADEEGEPPPASGKLGYSHCTETEVIESLGIIIYKALDYGLKENEERELSPPLEQLIDQMANTSEADGSNDEGYEAADEGPDEEDGERRSSAAIRSYQDVMKICAAHLPTESEAPNHYQAVCRALFAETMELHTFLTKIKSAKENLKKIQEMEKSDDCSTDLEDLKNADWARFWVQVMRDLRNGVKLKKVQQRQYNPLPIEYQLTPYEMLMDDIRCKRYTLRKVMVNGDIPPRLRKSAHEIILDFIRSRPPLNPASARKLKPTPPRPRSLHERILEEIKAERKLRPVSPEEIRRSRLDVTTPESPKNVGKSSVVNGDLTSQTKENGLGAAQPGPAQPKRLLKAPTLAELDSSDSEEETLHKSTSSSSASPSLYEDPVLEAMCSKKKPPKFLPISSTPQPERRQPPQRRHSIEKETPTNVRQFLPPSRQSSRSLVPRITSVWPRTPFRPLFSAIQTASLLSSHPFEAAMFGVAGAMYYLFERAFTSRWKPSKEEFCYPVECLALTVEEVMHIRQVLVKAELEKYQQYKDVYTALKKGKLCFCCRTRRFSFFTWSYTCQFCKRPVCSQCCKKMRLPSKPYSTLPIFSLGPSALQRGESCSRSEKPSTGHHRPLRSITRFSSKSKSVDKSDEELQFPKEFMEDWSTMEVCVDCKKFISEIISSSRRSLVLANKRARLKRKTQSFYMSSAGPSEYCPSERTINEI

Target ORF information:

RefSeq Version	XM_006254862.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus spire-type actin nucleation factor 1 (Spire1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006254862.3

ORF Insert Sequence:

ATGGCGCAGC CCTCAAGCCC GGGCGGCGAG GGGCCGCAGC TCGGCGCGAC AGGGGACCCG 
CGCGACGCGC TGAGCTTGGA AGAGATCCTG CGGCTCTACA ACCAGCCCAT CAACGAGGAG 
CAGGCGTGGG CCGTGTGCTT CCAGTGCTGC GGCTCCCTGC GCGCCGCCGC CCGCCGCCAG 
CCCCACCGCC GCGTGCGCTC GGCCGCGCAG ATCCGCGTCT GGAGGGACGG CGCCGTCACC 
CTGGCGCCCG CCGCGGACGA GGAGGGAGAG CCGCCCCCTG CCTCCGGTAA ATTGGGATAT 
TCACATTGTA CAGAAACAGA GGTTATCGAA TCCCTGGGAA TTATTATTTA TAAAGCACTG 
GACTATGGCT TGAAGGAAAA TGAAGAGAGG GAGCTGAGCC CTCCTCTCGA GCAGCTCATC 
GACCAAATGG CCAATACCTC GGAGGCGGAT GGAAGCAATG ATGAAGGGTA CGAGGCAGCA 
GATGAAGGCC CAGACGAGGA AGATGGAGAG AGGAGAAGCA GCGCAGCCAT TCGGTCCTAT 
CAAGATGTCA TGAAGATATG TGCTGCTCAT CTCCCCACTG AGTCGGAGGC ACCGAATCAT 
TATCAGGCAG TATGTCGGGC ACTGTTTGCA GAAACCATGG AACTGCATAC GTTTCTGACC 
AAAATTAAGA GTGCGAAGGA GAACCTTAAG AAGATTCAAG AAATGGAAAA GAGTGATGAC 
TGTAGCACAG ATCTGGAGGA CCTGAAAAAT GCAGACTGGG CACGGTTCTG GGTACAGGTG 
ATGAGGGATT TGCGAAATGG GGTAAAACTT AAGAAAGTCC AACAGCGGCA GTACAACCCA 
TTGCCCATTG AATACCAGCT CACCCCGTAT GAGATGCTCA TGGACGACAT TCGGTGCAAA 
AGATACACCT TGAGAAAAGT AATGGTAAAT GGTGACATTC CCCCTCGGTT AAGAAAGAGT 
GCTCATGAGA TCATCCTTGA CTTCATCAGA TCAAGACCCC CTTTAAATCC AGCTTCAGCC 
AGAAAACTGA AACCAACCCC ACCACGGCCA CGGAGCCTCC ATGAAAGAAT ACTAGAAGAA 
ATTAAAGCAG AAAGAAAGCT TCGGCCTGTC TCACCAGAAG AAATTAGACG GAGCAGACTA 
GATGTTACTA CACCAGAATC TCCAAAGAAT GTGGGGAAAT CATCTGTGGT GAATGGAGAC 
TTAACATCTC AAACGAAAGA AAATGGGCTG GGTGCTGCCC AGCCGGGGCC TGCACAGCCA 
AAGAGGCTCC TCAAAGCCCC GACGCTGGCT GAGCTGGACA GCTCTGACTC TGAGGAGGAA 
ACTCTGCACA AGTCAACCAG CAGCAGCAGT GCATCTCCCT CCCTCTATGA GGACCCAGTA 
CTGGAGGCCA TGTGCTCCAA GAAGAAGCCT CCAAAATTCT TGCCCATCTC ATCAACACCC 
CAGCCCGAGA GAAGGCAACC ACCACAGAGG CGCCACTCCA TTGAGAAGGA AACTCCTACA 
AATGTGAGGC AGTTTCTGCC CCCATCCAGG CAGAGCTCAC GTTCTCTTGT TCCTAGGATA 
ACCAGTGTAT GGCCAAGGAC GCCTTTCCGA CCACTTTTCT CTGCCATACA AACAGCTTCT 
CTGCTGAGCT CTCATCCTTT TGAAGCAGCC ATGTTTGGGG TAGCAGGGGC TATGTATTAT 
CTGTTTGAGA GAGCTTTTAC CAGCAGGTGG AAACCCTCAA AGGAGGAATT CTGCTACCCA 
GTGGAATGCC TCGCTCTCAC TGTGGAGGAA GTGATGCACA TTCGTCAGGT CCTGGTGAAA 
GCGGAGCTGG AAAAGTACCA ACAGTATAAG GATGTCTACA CTGCCCTGAA AAAAGGAAAG 
CTCTGCTTCT GTTGCCGAAC CCGGAGGTTT TCCTTCTTCA CCTGGTCCTA CACCTGTCAG 
TTCTGTAAGA GGCCCGTGTG CTCACAGTGT TGCAAAAAGA TGCGGCTGCC TTCGAAGCCA 
TACTCCACTT TACCTATCTT CTCTTTGGGA CCTTCTGCCT TGCAAAGAGG GGAAAGTTGT 
TCGAGGTCAG AAAAACCTTC TACTGGTCAC CATCGGCCGC TTCGAAGTAT TACCAGATTC 
TCTTCAAAAT CAAAGTCTGT GGACAAATCA GATGAAGAAC TCCAGTTTCC CAAAGAGTTC 
ATGGAAGATT GGAGCACAAT GGAGGTGTGT GTAGACTGCA AAAAGTTCAT TTCAGAGATC 
ATCAGCTCAA GCCGGCGCAG CCTGGTGTTG GCCAACAAAA GAGCCAGGTT GAAAAGGAAG 
ACACAGTCTT TCTACATGTC CTCAGCGGGC CCCTCGGAGT ACTGCCCTTC AGAACGGACT 
ATCAACGAGA TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Spire1 ( XM_006254863.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006254925.1 Rattus norvegicus spire-type actin nucleation factor 1 (Spire1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ORa38462
Clone ID Related Accession (Same CDS sequence)	XM_006254863.3
Accession Version	XM_006254863.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2223bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	protein spire homolog 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005117.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006254863.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCAGC CCTCAAGCCC GGGCGGCGAG GGGCCGCAGC TCGGCGCGAC AGGGGACCCG 
CGCGACGCGC TGAGCTTGGA AGAGATCCTG CGGCTCTACA ACCAGCCCAT CAACGAGGAG 
CAGGCGTGGG CCGTGTGCTT CCAGTGCTGC GGCTCCCTGC GCGCCGCCGC CCGCCGCCAG 
CCCCACCGCC GCGTGCGCTC GGCCGCGCAG ATCCGCGTCT GGAGGGACGG CGCCGTCACC 
CTGGCGCCCG CCGCGGACGA GGAGGGAGAG CCGCCCCCTG CCTCCGGTAA ATTGGGATAT 
TCACATTGTA CAGAAACAGA GGTTATCGAA TCCCTGGGAA TTATTATTTA TAAAGCACTG 
GACTATGGCT TGAAGGAAAA TGAAGAGAGG GAGCTGAGCC CTCCTCTCGA GCAGCTCATC 
GACCAAATGG CCAATACCTC GGAGGCGGAT GGAAGCAATG ATGAAGGGTA CGAGGCAGCA 
GATGAAGGCC CAGACGAGGA AGATGGAGAG AGGAGAAGCA GCGCAGCCAT TCGGTCCTAT 
CAAGATGTCA TGAAGATATG TGCTGCTCAT CTCCCCACTG AGTCGGAGGC ACCGAATCAT 
TATCAGGCAG TATGTCGGGC ACTGTTTGCA GAAACCATGG AACTGCATAC GTTTCTGACC 
AAAATTAAGA GTGCGAAGGA GAACCTTAAG AAGATTCAAG AAATGGAAAA GAGTGATGAC 
TGTAGCACAG ATCTGGAGGA CCTGAAAAAT GCAGACTGGG CACGGTTCTG GGTACAGGTG 
ATGAGGGATT TGCGAAATGG GGTAAAACTT AAGAAAGTCC AACAGCGGCA GTACAACCCA 
TTGCCCATTG AATACCAGCT CACCCCGTAT GAGATGCTCA TGGACGACAT TCGGTGCAAA 
AGATACACCT TGAGAAAAGT AATGGTAAAT GGTGACATTC CCCCTCGGTT AAGAAAGAGT 
GCTCATGAGA TCATCCTTGA CTTCATCAGA TCAAGACCCC CTTTAAATCC AGCTTCAGCC 
AGAAAACTGA AACCAACCCC ACCACGGCCA CGGAGCCTCC ATGAAAGAAT ACTAGAAGAA 
ATTAAAGCAG AAAGAAAGCT TCGGCCTGTC TCACCAGAAG AAATTAGACG GAGCAGACTA 
GCAGTTCGGC CACTTAGCAT GTCTCACAGT TTTGACTTGT CAGATGTTAC TACACCAGAA 
TCTCCAAAGA ATGTGGGGAA ATCATCTGTG GTGAATGGAG ACTTAACATC TCAAACGAAA 
GAAAATGGGC TGGGTGCTGC CCAGCCGGGG CCTGCACAGC CAAAGAGGCT CCTCAAAGCC 
CCGACGCTGG CTGAGCTGGA CAGCTCTGAC TCTGAGGAGG AAACTCTGCA CAAGTCAACC 
AGCAGCAGCA GTGCATCTCC CTCCCTCTAT GAGGACCCAG TACTGGAGGC CATGTGCTCC 
AAGAAGAAGC CTCCAAAATT CTTGCCCATC TCATCAACAC CCCAGCCCGA GAGAAGGCAA 
CCACCACAGA GGCGCCACTC CATTGAGAAG GAAACTCCTA CAAATGTGAG GCAGTTTCTG 
CCCCCATCCA GGCAGAGCTC ACGTTCTCTT GAGGAATTCT GCTACCCAGT GGAATGCCTC 
GCTCTCACTG TGGAGGAAGT GATGCACATT CGTCAGGTCC TGGTGAAAGC GGAGCTGGAA 
AAGTACCAAC AGTATAAGGA TGTCTACACT GCCCTGAAAA AAGGAAAGCT CTGCTTCTGT 
TGCCGAACCC GGAGGTTTTC CTTCTTCACC TGGTCCTACA CCTGTCAGTT CTGTAAGAGG 
CCCGTGTGCT CACAGTGTTG CAAAAAGATG CGGCTGCCTT CGAAGCCATA CTCCACTTTA 
CCTATCTTCT CTTTGGGACC TTCTGCCTTG CAAAGAGGGG AAAGTTGTTC GAGGTCAGAA 
AAACCTTCTA CTGGTCACCA TCGGCCGCTT CGAAGTATTA CCAGATTCTC TTCAAAATCA 
AAGTCTGTGG ACAAATCAGA TGAAGAACTC CAGTTTCCCA AAGAGTTCAT GGAAGATTGG 
AGCACAATGG AGGTGTGTGT AGACTGCAAA AAGTTCATTT CAGAGATCAT CAGCTCAAGC 
CGGCGCAGCC TGGTGTTGGC CAACAAAAGA GCCAGGTTGA AAAGGAAGAC ACAGTCTTTC 
TACATGTCCT CAGCGGGCCC CTCGGAGTAC TGCCCTTCAG AACGGACTAT CAACGAGATC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006254925.1
CDS	209..2431
Translation	MAQPSSPGGEGPQLGATGDPRDALSLEEILRLYNQPINEEQAWAVCFQCCGSLRAAARRQPHRRVRSAAQIRVWRDGAVTLAPAADEEGEPPPASGKLGYSHCTETEVIESLGIIIYKALDYGLKENEERELSPPLEQLIDQMANTSEADGSNDEGYEAADEGPDEEDGERRSSAAIRSYQDVMKICAAHLPTESEAPNHYQAVCRALFAETMELHTFLTKIKSAKENLKKIQEMEKSDDCSTDLEDLKNADWARFWVQVMRDLRNGVKLKKVQQRQYNPLPIEYQLTPYEMLMDDIRCKRYTLRKVMVNGDIPPRLRKSAHEIILDFIRSRPPLNPASARKLKPTPPRPRSLHERILEEIKAERKLRPVSPEEIRRSRLAVRPLSMSHSFDLSDVTTPESPKNVGKSSVVNGDLTSQTKENGLGAAQPGPAQPKRLLKAPTLAELDSSDSEEETLHKSTSSSSASPSLYEDPVLEAMCSKKKPPKFLPISSTPQPERRQPPQRRHSIEKETPTNVRQFLPPSRQSSRSLEEFCYPVECLALTVEEVMHIRQVLVKAELEKYQQYKDVYTALKKGKLCFCCRTRRFSFFTWSYTCQFCKRPVCSQCCKKMRLPSKPYSTLPIFSLGPSALQRGESCSRSEKPSTGHHRPLRSITRFSSKSKSVDKSDEELQFPKEFMEDWSTMEVCVDCKKFISEIISSSRRSLVLANKRARLKRKTQSFYMSSAGPSEYCPSERTINEI

Target ORF information:

RefSeq Version	XM_006254863.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus spire-type actin nucleation factor 1 (Spire1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006254863.3

ORF Insert Sequence:

ATGGCGCAGC CCTCAAGCCC GGGCGGCGAG GGGCCGCAGC TCGGCGCGAC AGGGGACCCG 
CGCGACGCGC TGAGCTTGGA AGAGATCCTG CGGCTCTACA ACCAGCCCAT CAACGAGGAG 
CAGGCGTGGG CCGTGTGCTT CCAGTGCTGC GGCTCCCTGC GCGCCGCCGC CCGCCGCCAG 
CCCCACCGCC GCGTGCGCTC GGCCGCGCAG ATCCGCGTCT GGAGGGACGG CGCCGTCACC 
CTGGCGCCCG CCGCGGACGA GGAGGGAGAG CCGCCCCCTG CCTCCGGTAA ATTGGGATAT 
TCACATTGTA CAGAAACAGA GGTTATCGAA TCCCTGGGAA TTATTATTTA TAAAGCACTG 
GACTATGGCT TGAAGGAAAA TGAAGAGAGG GAGCTGAGCC CTCCTCTCGA GCAGCTCATC 
GACCAAATGG CCAATACCTC GGAGGCGGAT GGAAGCAATG ATGAAGGGTA CGAGGCAGCA 
GATGAAGGCC CAGACGAGGA AGATGGAGAG AGGAGAAGCA GCGCAGCCAT TCGGTCCTAT 
CAAGATGTCA TGAAGATATG TGCTGCTCAT CTCCCCACTG AGTCGGAGGC ACCGAATCAT 
TATCAGGCAG TATGTCGGGC ACTGTTTGCA GAAACCATGG AACTGCATAC GTTTCTGACC 
AAAATTAAGA GTGCGAAGGA GAACCTTAAG AAGATTCAAG AAATGGAAAA GAGTGATGAC 
TGTAGCACAG ATCTGGAGGA CCTGAAAAAT GCAGACTGGG CACGGTTCTG GGTACAGGTG 
ATGAGGGATT TGCGAAATGG GGTAAAACTT AAGAAAGTCC AACAGCGGCA GTACAACCCA 
TTGCCCATTG AATACCAGCT CACCCCGTAT GAGATGCTCA TGGACGACAT TCGGTGCAAA 
AGATACACCT TGAGAAAAGT AATGGTAAAT GGTGACATTC CCCCTCGGTT AAGAAAGAGT 
GCTCATGAGA TCATCCTTGA CTTCATCAGA TCAAGACCCC CTTTAAATCC AGCTTCAGCC 
AGAAAACTGA AACCAACCCC ACCACGGCCA CGGAGCCTCC ATGAAAGAAT ACTAGAAGAA 
ATTAAAGCAG AAAGAAAGCT TCGGCCTGTC TCACCAGAAG AAATTAGACG GAGCAGACTA 
GCAGTTCGGC CACTTAGCAT GTCTCACAGT TTTGACTTGT CAGATGTTAC TACACCAGAA 
TCTCCAAAGA ATGTGGGGAA ATCATCTGTG GTGAATGGAG ACTTAACATC TCAAACGAAA 
GAAAATGGGC TGGGTGCTGC CCAGCCGGGG CCTGCACAGC CAAAGAGGCT CCTCAAAGCC 
CCGACGCTGG CTGAGCTGGA CAGCTCTGAC TCTGAGGAGG AAACTCTGCA CAAGTCAACC 
AGCAGCAGCA GTGCATCTCC CTCCCTCTAT GAGGACCCAG TACTGGAGGC CATGTGCTCC 
AAGAAGAAGC CTCCAAAATT CTTGCCCATC TCATCAACAC CCCAGCCCGA GAGAAGGCAA 
CCACCACAGA GGCGCCACTC CATTGAGAAG GAAACTCCTA CAAATGTGAG GCAGTTTCTG 
CCCCCATCCA GGCAGAGCTC ACGTTCTCTT GAGGAATTCT GCTACCCAGT GGAATGCCTC 
GCTCTCACTG TGGAGGAAGT GATGCACATT CGTCAGGTCC TGGTGAAAGC GGAGCTGGAA 
AAGTACCAAC AGTATAAGGA TGTCTACACT GCCCTGAAAA AAGGAAAGCT CTGCTTCTGT 
TGCCGAACCC GGAGGTTTTC CTTCTTCACC TGGTCCTACA CCTGTCAGTT CTGTAAGAGG 
CCCGTGTGCT CACAGTGTTG CAAAAAGATG CGGCTGCCTT CGAAGCCATA CTCCACTTTA 
CCTATCTTCT CTTTGGGACC TTCTGCCTTG CAAAGAGGGG AAAGTTGTTC GAGGTCAGAA 
AAACCTTCTA CTGGTCACCA TCGGCCGCTT CGAAGTATTA CCAGATTCTC TTCAAAATCA 
AAGTCTGTGG ACAAATCAGA TGAAGAACTC CAGTTTCCCA AAGAGTTCAT GGAAGATTGG 
AGCACAATGG AGGTGTGTGT AGACTGCAAA AAGTTCATTT CAGAGATCAT CAGCTCAAGC 
CGGCGCAGCC TGGTGTTGGC CAACAAAAGA GCCAGGTTGA AAAGGAAGAC ACAGTCTTTC 
TACATGTCCT CAGCGGGCCC CTCGGAGTAC TGCCCTTCAG AACGGACTAT CAACGAGATC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Spire1 ( XM_006254864.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006254926.1 Rattus norvegicus spire-type actin nucleation factor 1 (Spire1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	ORa38463
Clone ID Related Accession (Same CDS sequence)	XM_006254864.3
Accession Version	XM_006254864.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2181bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	protein spire homolog 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005117.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006254864.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCAGC CCTCAAGCCC GGGCGGCGAG GGGCCGCAGC TCGGCGCGAC AGGGGACCCG 
CGCGACGCGC TGAGCTTGGA AGAGATCCTG CGGCTCTACA ACCAGCCCAT CAACGAGGAG 
CAGGCGTGGG CCGTGTGCTT CCAGTGCTGC GGCTCCCTGC GCGCCGCCGC CCGCCGCCAG 
CCCCACCGCC GCGTGCGCTC GGCCGCGCAG ATCCGCGTCT GGAGGGACGG CGCCGTCACC 
CTGGCGCCCG CCGCGGACGA GGAGGGAGAG CCGCCCCCTG CCTCCGGTAA ATTGGGATAT 
TCACATTGTA CAGAAACAGA GGTTATCGAA TCCCTGGGAA TTATTATTTA TAAAGCACTG 
GACTATGGCT TGAAGGAAAA TGAAGAGAGG GAGCTGAGCC CTCCTCTCGA GCAGCTCATC 
GACCAAATGG CCAATACCTC GGAGGCGGAT GGAAGCAATG ATGAAGGGTA CGAGGCAGCA 
GATGAAGGCC CAGACGAGGA AGATGGAGAG AGGAGAAGCA GCGCAGCCAT TCGGTCCTAT 
CAAGATGTCA TGAAGATATG TGCTGCTCAT CTCCCCACTG AGTCGGAGGC ACCGAATCAT 
TATCAGGCAG TATGTCGGGC ACTGTTTGCA GAAACCATGG AACTGCATAC GTTTCTGACC 
AAAATTAAGA GTGCGAAGGA GAACCTTAAG AAGATTCAAG AAATGGAAAA GAGTGATGAC 
TGTAGCACAG ATCTGGAGGA CCTGAAAAAT GCAGACTGGG CACGGTTCTG GGTACAGGTG 
ATGAGGGATT TGCGAAATGG GGTAAAACTT AAGAAAGTCC AACAGCGGCA GTACAACCCA 
TTGCCCATTG AATACCAGCT CACCCCGTAT GAGATGCTCA TGGACGACAT TCGGTGCAAA 
AGATACACCT TGAGAAAAGT AATGGTAAAT GGTGACATTC CCCCTCGGTT AAGAAAGAGT 
GCTCATGAGA TCATCCTTGA CTTCATCAGA TCAAGACCCC CTTTAAATCC AGCTTCAGCC 
AGAAAACTGA AACCAACCCC ACCACGGCCA CGGAGCCTCC ATGAAAGAAT ACTAGAAGAA 
ATTAAAGCAG AAAGAAAGCT TCGGCCTGTC TCACCAGAAG AAATTAGACG GAGCAGACTA 
GATGTTACTA CACCAGAATC TCCAAAGAAT GTGGGGAAAT CATCTGTGGT GAATGGAGAC 
TTAACATCTC AAACGAAAGA AAATGGGCTG GGTGCTGCCC AGCCGGGGCC TGCACAGCCA 
AAGAGGCTCC TCAAAGCCCC GACGCTGGCT GAGCTGGACA GCTCTGACTC TGAGGAGGAA 
ACTCTGCACA AGTCAACCAG CAGCAGCAGT GCATCTCCCT CCCTCTATGA GGACCCAGTA 
CTGGAGGCCA TGTGCTCCAA GAAGAAGCCT CCAAAATTCT TGCCCATCTC ATCAACACCC 
CAGCCCGAGA GAAGGCAACC ACCACAGAGG CGCCACTCCA TTGAGAAGGA AACTCCTACA 
AATGTGAGGC AGTTTCTGCC CCCATCCAGG CAGAGCTCAC GTTCTCTTGA GGAATTCTGC 
TACCCAGTGG AATGCCTCGC TCTCACTGTG GAGGAAGTGA TGCACATTCG TCAGGTCCTG 
GTGAAAGCGG AGCTGGAAAA GTACCAACAG TATAAGGATG TCTACACTGC CCTGAAAAAA 
GGAAAGCTCT GCTTCTGTTG CCGAACCCGG AGGTTTTCCT TCTTCACCTG GTCCTACACC 
TGTCAGTTCT GTAAGAGGCC CGTGTGCTCA CAGTGTTGCA AAAAGATGCG GCTGCCTTCG 
AAGCCATACT CCACTTTACC TATCTTCTCT TTGGGACCTT CTGCCTTGCA AAGAGGGGAA 
AGTTGTTCGA GGTCAGAAAA ACCTTCTACT GGTCACCATC GGCCGCTTCG AAGTATTACC 
AGATTCTCTT CAAAATCAAA GTCTGTGGAC AAATCAGATG AAGAACTCCA GTTTCCCAAA 
GAGTTCATGG AAGATTGGAG CACAATGGAG GTGTGTGTAG ACTGCAAAAA GTTCATTTCA 
GAGATCATCA GCTCAAGCCG GCGCAGCCTG GTGTTGGCCA ACAAAAGAGC CAGGTTGAAA 
AGGAAGACAC AGTCTTTCTA CATGTCCTCA GCGGGCCCCT CGGAGTACTG CCCTTCAGAA 
CGGACTATCA ACGAGATCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006254926.1
CDS	209..2389
Translation	MAQPSSPGGEGPQLGATGDPRDALSLEEILRLYNQPINEEQAWAVCFQCCGSLRAAARRQPHRRVRSAAQIRVWRDGAVTLAPAADEEGEPPPASGKLGYSHCTETEVIESLGIIIYKALDYGLKENEERELSPPLEQLIDQMANTSEADGSNDEGYEAADEGPDEEDGERRSSAAIRSYQDVMKICAAHLPTESEAPNHYQAVCRALFAETMELHTFLTKIKSAKENLKKIQEMEKSDDCSTDLEDLKNADWARFWVQVMRDLRNGVKLKKVQQRQYNPLPIEYQLTPYEMLMDDIRCKRYTLRKVMVNGDIPPRLRKSAHEIILDFIRSRPPLNPASARKLKPTPPRPRSLHERILEEIKAERKLRPVSPEEIRRSRLDVTTPESPKNVGKSSVVNGDLTSQTKENGLGAAQPGPAQPKRLLKAPTLAELDSSDSEEETLHKSTSSSSASPSLYEDPVLEAMCSKKKPPKFLPISSTPQPERRQPPQRRHSIEKETPTNVRQFLPPSRQSSRSLEEFCYPVECLALTVEEVMHIRQVLVKAELEKYQQYKDVYTALKKGKLCFCCRTRRFSFFTWSYTCQFCKRPVCSQCCKKMRLPSKPYSTLPIFSLGPSALQRGESCSRSEKPSTGHHRPLRSITRFSSKSKSVDKSDEELQFPKEFMEDWSTMEVCVDCKKFISEIISSSRRSLVLANKRARLKRKTQSFYMSSAGPSEYCPSERTINEI

Target ORF information:

RefSeq Version	XM_006254864.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus spire-type actin nucleation factor 1 (Spire1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006254864.3

ORF Insert Sequence:

ATGGCGCAGC CCTCAAGCCC GGGCGGCGAG GGGCCGCAGC TCGGCGCGAC AGGGGACCCG 
CGCGACGCGC TGAGCTTGGA AGAGATCCTG CGGCTCTACA ACCAGCCCAT CAACGAGGAG 
CAGGCGTGGG CCGTGTGCTT CCAGTGCTGC GGCTCCCTGC GCGCCGCCGC CCGCCGCCAG 
CCCCACCGCC GCGTGCGCTC GGCCGCGCAG ATCCGCGTCT GGAGGGACGG CGCCGTCACC 
CTGGCGCCCG CCGCGGACGA GGAGGGAGAG CCGCCCCCTG CCTCCGGTAA ATTGGGATAT 
TCACATTGTA CAGAAACAGA GGTTATCGAA TCCCTGGGAA TTATTATTTA TAAAGCACTG 
GACTATGGCT TGAAGGAAAA TGAAGAGAGG GAGCTGAGCC CTCCTCTCGA GCAGCTCATC 
GACCAAATGG CCAATACCTC GGAGGCGGAT GGAAGCAATG ATGAAGGGTA CGAGGCAGCA 
GATGAAGGCC CAGACGAGGA AGATGGAGAG AGGAGAAGCA GCGCAGCCAT TCGGTCCTAT 
CAAGATGTCA TGAAGATATG TGCTGCTCAT CTCCCCACTG AGTCGGAGGC ACCGAATCAT 
TATCAGGCAG TATGTCGGGC ACTGTTTGCA GAAACCATGG AACTGCATAC GTTTCTGACC 
AAAATTAAGA GTGCGAAGGA GAACCTTAAG AAGATTCAAG AAATGGAAAA GAGTGATGAC 
TGTAGCACAG ATCTGGAGGA CCTGAAAAAT GCAGACTGGG CACGGTTCTG GGTACAGGTG 
ATGAGGGATT TGCGAAATGG GGTAAAACTT AAGAAAGTCC AACAGCGGCA GTACAACCCA 
TTGCCCATTG AATACCAGCT CACCCCGTAT GAGATGCTCA TGGACGACAT TCGGTGCAAA 
AGATACACCT TGAGAAAAGT AATGGTAAAT GGTGACATTC CCCCTCGGTT AAGAAAGAGT 
GCTCATGAGA TCATCCTTGA CTTCATCAGA TCAAGACCCC CTTTAAATCC AGCTTCAGCC 
AGAAAACTGA AACCAACCCC ACCACGGCCA CGGAGCCTCC ATGAAAGAAT ACTAGAAGAA 
ATTAAAGCAG AAAGAAAGCT TCGGCCTGTC TCACCAGAAG AAATTAGACG GAGCAGACTA 
GATGTTACTA CACCAGAATC TCCAAAGAAT GTGGGGAAAT CATCTGTGGT GAATGGAGAC 
TTAACATCTC AAACGAAAGA AAATGGGCTG GGTGCTGCCC AGCCGGGGCC TGCACAGCCA 
AAGAGGCTCC TCAAAGCCCC GACGCTGGCT GAGCTGGACA GCTCTGACTC TGAGGAGGAA 
ACTCTGCACA AGTCAACCAG CAGCAGCAGT GCATCTCCCT CCCTCTATGA GGACCCAGTA 
CTGGAGGCCA TGTGCTCCAA GAAGAAGCCT CCAAAATTCT TGCCCATCTC ATCAACACCC 
CAGCCCGAGA GAAGGCAACC ACCACAGAGG CGCCACTCCA TTGAGAAGGA AACTCCTACA 
AATGTGAGGC AGTTTCTGCC CCCATCCAGG CAGAGCTCAC GTTCTCTTGA GGAATTCTGC 
TACCCAGTGG AATGCCTCGC TCTCACTGTG GAGGAAGTGA TGCACATTCG TCAGGTCCTG 
GTGAAAGCGG AGCTGGAAAA GTACCAACAG TATAAGGATG TCTACACTGC CCTGAAAAAA 
GGAAAGCTCT GCTTCTGTTG CCGAACCCGG AGGTTTTCCT TCTTCACCTG GTCCTACACC 
TGTCAGTTCT GTAAGAGGCC CGTGTGCTCA CAGTGTTGCA AAAAGATGCG GCTGCCTTCG 
AAGCCATACT CCACTTTACC TATCTTCTCT TTGGGACCTT CTGCCTTGCA AAGAGGGGAA 
AGTTGTTCGA GGTCAGAAAA ACCTTCTACT GGTCACCATC GGCCGCTTCG AAGTATTACC 
AGATTCTCTT CAAAATCAAA GTCTGTGGAC AAATCAGATG AAGAACTCCA GTTTCCCAAA 
GAGTTCATGG AAGATTGGAG CACAATGGAG GTGTGTGTAG ACTGCAAAAA GTTCATTTCA 
GAGATCATCA GCTCAAGCCG GCGCAGCCTG GTGTTGGCCA ACAAAAGAGC CAGGTTGAAA 
AGGAAGACAC AGTCTTTCTA CATGTCCTCA GCGGGCCCCT CGGAGTACTG CCCTTCAGAA 
CGGACTATCA ACGAGATCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Spire1 ( XM_017600946.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017456435.1 Rattus norvegicus spire-type actin nucleation factor 1 (Spire1), transcript variant X2, mRNA.

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CloneID	ORa59261
Clone ID Related Accession (Same CDS sequence)	XM_017600946.1
Accession Version	XM_017600946.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2394bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	protein spire homolog 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005117.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCAGC CCTCAAGCCC GGGCGGCGAG GGGCCGCAGC TCGGCGCGAC AGGGGACCCG 
CGCGACGCGC TGAGCTTGGA AGAGATCCTG CGGCTCTACA ACCAGCCCAT CAACGAGGAG 
CAGGCGTGGG CCGTGTGCTT CCAGTGCTGC GGCTCCCTGC GCGCCGCCGC CCGCCGCCAG 
CCCCACCGCC GCGTGCGCTC GGCCGCGCAG ATCCGCGTCT GGAGGGACGG CGCCGTCACC 
CTGGCGCCCG CCGCGGACGA GGAGGGAGAG CCGCCCCCTG CCTCCGGTAA ATTGGGATAT 
TCACATTGTA CAGAAACAGA GGTTATCGAA TCCCTGGGAA TTATTATTTA TAAAGCACTG 
GACTATGGCT TGAAGGAAAA TGAAGAGAGG GAGCTGAGCC CTCCTCTCGA GCAGCTCATC 
GACCAAATGG CCAATACCTC GGAGGCGGAT GGAAGCAATG ATGAAGGGTA CGAGGCAGCA 
GATGAAGGCC CAGACGAGGA AGATGGAGAG AGGAGAAGCA GCGCAGCCAT TCGGTCCTAT 
CAAGATGTCA TGAAGATATG TGCTGCTCAT CTCCCCACTG AGTCGGAGGC ACCGAATCAT 
TATCAGGCAG TATGTCGGGC ACTGTTTGCA GAAACCATGG AACTGCATAC GTTTCTGACC 
AAAATTAAGA GTGCGAAGGA GAACCTTAAG AAGATTCAAG AAATGGAAAA GAGTGATGAC 
TGTAGCACAG ATCTGGAGGA CCTGAAAAAT GCAGACTGGG CACGGTTCTG GGTACAGGTG 
ATGAGGGATT TGCGAAATGG GGTAAAACTT AAGAAAGTCC AACAGCGGCA GTACAACCCA 
TTGCCCATTG AATACCAGCT CACCCCGTAT GAGATGCTCA TGGACGACAT TCGGTGCAAA 
AGATACACCT TGAGAAAAGT AATGGTAAAT GGTGACATTC CCCCTCGGTT AAGAAAGAGT 
GCTCATGAGA TCATCCTTGA CTTCATCAGA TCAAGACCCC CTTTAAATCC AGCTTCAGCC 
AGAAAACTGA AACCAACCCC ACCACGGCCA CGGAGCCTCC ATGAAAGAAT ACTAGAAGAA 
ATTAAAGCAG AAAGAAAGCT TCGGCCTGTC TCACCAGAAG AAATTAGACG GAGCAGACTA 
GTTCGGCCAC TTAGCATGTC TCACAGTTTT GACTTGTCAG ATGTTACTAC ACCAGAATCT 
CCAAAGAATG TGGGGAAATC ATCTGTGGTG AATGGAGACT TAACATCTCA AACGAAAGAA 
AATGGGCTGG GTGCTGCCCA GCCGGGGCCT GCACAGCCAA AGAGGCTCCT CAAAGCCCCG 
ACGCTGGCTG AGCTGGACAG CTCTGACTCT GAGGAGGAAA CTCTGCACAA GTCAACCAGC 
AGCAGCAGTG CATCTCCCTC CCTCTATGAG GACCCAGTAC TGGAGGCCAT GTGCTCCAAG 
AAGAAGCCTC CAAAATTCTT GCCCATCTCA TCAACACCCC AGCCCGAGAG AAGGCAACCA 
CCACAGAGGC GCCACTCCAT TGAGAAGGAA ACTCCTACAA ATGTGAGGCA GTTTCTGCCC 
CCATCCAGGC AGAGCTCACG TTCTCTTGTT CCTAGGATAA CCAGTGTATG GCCAAGGACG 
CCTTTCCGAC CACTTTTCTC TGCCATACAA ACAGCTTCTC TGCTGAGCTC TCATCCTTTT 
GAAGCAGCCA TGTTTGGGGT AGCAGGGGCT ATGTATTATC TGTTTGAGAG AGCTTTTACC 
AGCAGGTGGA AACCCTCAAA GGAGGAATTC TGCTACCCAG TGGAATGCCT CGCTCTCACT 
GTGGAGGAAG TGATGCACAT TCGTCAGGTC CTGGTGAAAG CGGAGCTGGA AAAGTACCAA 
CAGTATAAGG ATGTCTACAC TGCCCTGAAA AAAGGAAAGC TCTGCTTCTG TTGCCGAACC 
CGGAGGTTTT CCTTCTTCAC CTGGTCCTAC ACCTGTCAGT TCTGTAAGAG GCCCGTGTGC 
TCACAGTGTT GCAAAAAGAT GCGGCTGCCT TCGAAGCCAT ACTCCACTTT ACCTATCTTC 
TCTTTGGGAC CTTCTGCCTT GCAAAGAGGG GAAAGTTGTT CGAGGTCAGA AAAACCTTCT 
ACTGGTCACC ATCGGCCGCT TCGAAGTATT ACCAGATTCT CTTCAAAATC AAAGTCTGTG 
GACAAATCAG ATGAAGAACT CCAGTTTCCC AAAGAGTTCA TGGAAGATTG GAGCACAATG 
GAGGTGTGTG TAGACTGCAA AAAGTTCATT TCAGAGATCA TCAGCTCAAG CCGGCGCAGC 
CTGGTGTTGG CCAACAAAAG AGCCAGGTTG AAAAGGAAGA CACAGTCTTT CTACATGTCC 
TCAGCGGGCC CCTCGGAGTA CTGCCCTTCA GAACGGACTA TCAACGAGAT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017456435.1
CDS	211..2604
Translation	MAQPSSPGGEGPQLGATGDPRDALSLEEILRLYNQPINEEQAWAVCFQCCGSLRAAARRQPHRRVRSAAQIRVWRDGAVTLAPAADEEGEPPPASGKLGYSHCTETEVIESLGIIIYKALDYGLKENEERELSPPLEQLIDQMANTSEADGSNDEGYEAADEGPDEEDGERRSSAAIRSYQDVMKICAAHLPTESEAPNHYQAVCRALFAETMELHTFLTKIKSAKENLKKIQEMEKSDDCSTDLEDLKNADWARFWVQVMRDLRNGVKLKKVQQRQYNPLPIEYQLTPYEMLMDDIRCKRYTLRKVMVNGDIPPRLRKSAHEIILDFIRSRPPLNPASARKLKPTPPRPRSLHERILEEIKAERKLRPVSPEEIRRSRLVRPLSMSHSFDLSDVTTPESPKNVGKSSVVNGDLTSQTKENGLGAAQPGPAQPKRLLKAPTLAELDSSDSEEETLHKSTSSSSASPSLYEDPVLEAMCSKKKPPKFLPISSTPQPERRQPPQRRHSIEKETPTNVRQFLPPSRQSSRSLVPRITSVWPRTPFRPLFSAIQTASLLSSHPFEAAMFGVAGAMYYLFERAFTSRWKPSKEEFCYPVECLALTVEEVMHIRQVLVKAELEKYQQYKDVYTALKKGKLCFCCRTRRFSFFTWSYTCQFCKRPVCSQCCKKMRLPSKPYSTLPIFSLGPSALQRGESCSRSEKPSTGHHRPLRSITRFSSKSKSVDKSDEELQFPKEFMEDWSTMEVCVDCKKFISEIISSSRRSLVLANKRARLKRKTQSFYMSSAGPSEYCPSERTINEI

Target ORF information:

RefSeq Version	XM_017600946.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus spire-type actin nucleation factor 1 (Spire1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017600946.1

ORF Insert Sequence:

ATGGCGCAGC CCTCAAGCCC GGGCGGCGAG GGGCCGCAGC TCGGCGCGAC AGGGGACCCG 
CGCGACGCGC TGAGCTTGGA AGAGATCCTG CGGCTCTACA ACCAGCCCAT CAACGAGGAG 
CAGGCGTGGG CCGTGTGCTT CCAGTGCTGC GGCTCCCTGC GCGCCGCCGC CCGCCGCCAG 
CCCCACCGCC GCGTGCGCTC GGCCGCGCAG ATCCGCGTCT GGAGGGACGG CGCCGTCACC 
CTGGCGCCCG CCGCGGACGA GGAGGGAGAG CCGCCCCCTG CCTCCGGTAA ATTGGGATAT 
TCACATTGTA CAGAAACAGA GGTTATCGAA TCCCTGGGAA TTATTATTTA TAAAGCACTG 
GACTATGGCT TGAAGGAAAA TGAAGAGAGG GAGCTGAGCC CTCCTCTCGA GCAGCTCATC 
GACCAAATGG CCAATACCTC GGAGGCGGAT GGAAGCAATG ATGAAGGGTA CGAGGCAGCA 
GATGAAGGCC CAGACGAGGA AGATGGAGAG AGGAGAAGCA GCGCAGCCAT TCGGTCCTAT 
CAAGATGTCA TGAAGATATG TGCTGCTCAT CTCCCCACTG AGTCGGAGGC ACCGAATCAT 
TATCAGGCAG TATGTCGGGC ACTGTTTGCA GAAACCATGG AACTGCATAC GTTTCTGACC 
AAAATTAAGA GTGCGAAGGA GAACCTTAAG AAGATTCAAG AAATGGAAAA GAGTGATGAC 
TGTAGCACAG ATCTGGAGGA CCTGAAAAAT GCAGACTGGG CACGGTTCTG GGTACAGGTG 
ATGAGGGATT TGCGAAATGG GGTAAAACTT AAGAAAGTCC AACAGCGGCA GTACAACCCA 
TTGCCCATTG AATACCAGCT CACCCCGTAT GAGATGCTCA TGGACGACAT TCGGTGCAAA 
AGATACACCT TGAGAAAAGT AATGGTAAAT GGTGACATTC CCCCTCGGTT AAGAAAGAGT 
GCTCATGAGA TCATCCTTGA CTTCATCAGA TCAAGACCCC CTTTAAATCC AGCTTCAGCC 
AGAAAACTGA AACCAACCCC ACCACGGCCA CGGAGCCTCC ATGAAAGAAT ACTAGAAGAA 
ATTAAAGCAG AAAGAAAGCT TCGGCCTGTC TCACCAGAAG AAATTAGACG GAGCAGACTA 
GTTCGGCCAC TTAGCATGTC TCACAGTTTT GACTTGTCAG ATGTTACTAC ACCAGAATCT 
CCAAAGAATG TGGGGAAATC ATCTGTGGTG AATGGAGACT TAACATCTCA AACGAAAGAA 
AATGGGCTGG GTGCTGCCCA GCCGGGGCCT GCACAGCCAA AGAGGCTCCT CAAAGCCCCG 
ACGCTGGCTG AGCTGGACAG CTCTGACTCT GAGGAGGAAA CTCTGCACAA GTCAACCAGC 
AGCAGCAGTG CATCTCCCTC CCTCTATGAG GACCCAGTAC TGGAGGCCAT GTGCTCCAAG 
AAGAAGCCTC CAAAATTCTT GCCCATCTCA TCAACACCCC AGCCCGAGAG AAGGCAACCA 
CCACAGAGGC GCCACTCCAT TGAGAAGGAA ACTCCTACAA ATGTGAGGCA GTTTCTGCCC 
CCATCCAGGC AGAGCTCACG TTCTCTTGTT CCTAGGATAA CCAGTGTATG GCCAAGGACG 
CCTTTCCGAC CACTTTTCTC TGCCATACAA ACAGCTTCTC TGCTGAGCTC TCATCCTTTT 
GAAGCAGCCA TGTTTGGGGT AGCAGGGGCT ATGTATTATC TGTTTGAGAG AGCTTTTACC 
AGCAGGTGGA AACCCTCAAA GGAGGAATTC TGCTACCCAG TGGAATGCCT CGCTCTCACT 
GTGGAGGAAG TGATGCACAT TCGTCAGGTC CTGGTGAAAG CGGAGCTGGA AAAGTACCAA 
CAGTATAAGG ATGTCTACAC TGCCCTGAAA AAAGGAAAGC TCTGCTTCTG TTGCCGAACC 
CGGAGGTTTT CCTTCTTCAC CTGGTCCTAC ACCTGTCAGT TCTGTAAGAG GCCCGTGTGC 
TCACAGTGTT GCAAAAAGAT GCGGCTGCCT TCGAAGCCAT ACTCCACTTT ACCTATCTTC 
TCTTTGGGAC CTTCTGCCTT GCAAAGAGGG GAAAGTTGTT CGAGGTCAGA AAAACCTTCT 
ACTGGTCACC ATCGGCCGCT TCGAAGTATT ACCAGATTCT CTTCAAAATC AAAGTCTGTG 
GACAAATCAG ATGAAGAACT CCAGTTTCCC AAAGAGTTCA TGGAAGATTG GAGCACAATG 
GAGGTGTGTG TAGACTGCAA AAAGTTCATT TCAGAGATCA TCAGCTCAAG CCGGCGCAGC 
CTGGTGTTGG CCAACAAAAG AGCCAGGTTG AAAAGGAAGA CACAGTCTTT CTACATGTCC 
TCAGCGGGCC CCTCGGAGTA CTGCCCTTCA GAACGGACTA TCAACGAGAT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

DNA damage induces nuclear actin filament assembly by Formin -2 and Spire-? that promotes efficient DNA repair. [corrected].
eLife4e07735(2015 Aug)
Belin BJ,Lee T,Mullins RD

Enhanced fear expression in Spir-1 actin organizer mutant mice.
European journal of cell biology93(5-6)225-37(2014 May-Jun)
Pleiser S,Banchaabouchi MA,Samol-Wolf A,Farley D,Welz T,Wellbourne-Wood J,Gehring I,Linkner J,Faix J,Riemenschneider MJ,Dietrich S,Kerkhoff E

An actin-dependent mechanism for long-range vesicle transport.
Nature cell biology13(12)1431-6(2011 Oct)
Schuh M

Spire-type actin nucleators cooperate with Formin-2 to drive asymmetric oocyte division.
Current biology : CB21(11)955-60(2011 Jun)
Pfender S,Kuznetsov V,Pleiser S,Kerkhoff E,Schuh M

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Spire1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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