Naf1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Naf1
Entrez Gene ID	306387
Full Name	nuclear assembly factor 1 ribonucleoprotein
Synonyms	RGD1306802
General protein information	Preferred Names nuclear assembly factor 1 ribonucleoprotein Names H/ACA ribonucleoprotein complex non-core subunit NAF1 nuclear assembly factor 1 homolog
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 16 Map Location: 16p14

mRNA and Protein(s)

mRNA	Protein	Name
NM_001024772.1	NP_001019943.1	H/ACA ribonucleoprotein complex non-core subunit NAF1
XM_017600101.1	XP_017455590.1	H/ACA ribonucleoprotein complex non-core subunit NAF1 isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	NAF1	XP_517514.2
Bos taurus (cattle)	NAF1	NP_001069120.2
Homo sapiens (human)	NAF1	NP_612395.2
Rattus norvegicus (Norway rat)	Naf1	NP_001019943.1
	NAF1	XP_001098118.1
Mus musculus (house mouse)	Naf1	NP_001157036.1
Canis lupus familiaris (dog)	NAF1	XP_532709.2

Gene Ontology
Bibliography

Related articles in PubMed

Loss-of-function mutations in the RNA biogenesis factor NAF1 predispose to pulmonary fibrosis-emphysema.
Stanley SE, Gable DL, Wagner CL, Carlile TM, Hanumanthu VS, Podlevsky JD, Khalil SE, DeZern AE, Rojas-Duran MF, Applegate CD, Alder JK, Parry EM, Gilbert WV, Armanios M
Science translational medicine8(351)351ra107(2016 08)

Proteostatic control of telomerase function through TRiC-mediated folding of TCAB1.
Freund A, Zhong FL, Venteicher AS, Meng Z, Veenstra TD, Frydman J, Artandi SE
Cell159(6)1389-403(2014 Dec)

An enhanced H/ACA RNP assembly mechanism for human telomerase RNA.
Egan ED, Collins K
Molecular and cellular biology32(13)2428-39(2012 Jul)

The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts.
Baltz AG, Munschauer M, Schwanh?usser B, Vasile A, Murakawa Y, Schueler M, Youngs N, Penfold-Brown D, Drew K, Milek M, Wyler E, Bonneau R, Selbach M, Dieterich C, Landthaler M
Molecular cell46(5)674-90(2012 Jun)

Purification of human telomerase complexes identifies factors involved in telomerase biogenesis and telomere length regulation.
Fu D, Collins K
Molecular cell28(5)773-85(2007 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Naf1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Naf1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa42665	NM_001024772.1 Latest version!	Rattus norvegicus nuclear assembly factor 1 ribonucleoprotein (Naf1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$223.30 $319.00
ORa58801	XM_017600101.1 Latest version!	Rattus norvegicus nuclear assembly factor 1 ribonucleoprotein (Naf1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Naf1 ( NM_001024772.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001019943.1 Rattus norvegicus nuclear assembly factor 1 ribonucleoprotein (Naf1), mRNA.

Price & Availability↑

CloneID	ORa42665
Clone ID Related Accession (Same CDS sequence)	NM_001024772.1
Accession Version	NM_001024772.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1374bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Rattus norvegicus(Norway rat)
Product	H/ACA ribonucleoprotein complex non-core subunit NAF1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC094302.1. ##Evidence-Data-START## Transcript exon combination :: BC094302.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMD00052296, SAMD00052297 [ECO:0000348] ##Evidence-Data-END##

ATGGAGGTGG TGGAGGCTGC CGCGCAGCTG CAGACGCTCA AGTTCGGCGG CTCCGGCCCG 
GGATCCGCAG CATCGCCGCC ACCTGAGGAG CGCCCGGACG CCGAGCAGGC GGAGCAGGCC 
CCGCCGGCAG GGGAGCAGCC GCCGCCAGCC CCGAGCTCGG ATGCTGGTGA AAACCCACCG 
CCGCCGAGCG GGCCCTGCGC CACCGCACCG GACGCCGCGG AGCCGCTGCC AGAGCTGCAG 
GACTCGGACT CATCGGACTC GGATTCGGAC AGTGAAACGG ATTCGGATAG CTCAAGCTCT 
TCCTCTTCCT CATCTTCATC ATCTTCCTCC TGTGGATCGC TTCCTCCTGT GTTGTCAGAC 
GGAGAGGAGG ATGTCCAAGT TGAGAAAGAA AATAAGAATT TTCCTCTTAA AACAAAAGAT 
GAATTGCTTC TTAATGAACT ACCTTCTGTG GAAGAACTCA CTGTTACTCT GCCTGAAGAC 
ATTGCACTAA AGCCTCTTGG GAAGGTTTCG AGCATTATTG AACAGTTAGT GATAATTGAG 
TCTATGACTA ACATACCTCC AGTTAATGAG GATACTGTCA TTTTCAAAAG TGATCGACAA 
GCAGCAGGGA AGATATTTGA GATCTTTGGA CCTGTTGCCC ATCCATTTTA TGTGTTACGG 
TTCAATTCTT CAGAGCACAT TGAGAGTAAA GGTATCAAAA TAAAGGATAC TATGTATTTT 
GCTCCATCAA TGAAAGACTT CACTCAGTAT ATATTCACAG AAAAACTCAA ACAGGACAGA 
GGATCTGATG CTTCTTGGAA GAATGATCAG GAACCACCTC CAGAAGCCTT GGATTTCAGT 
GATGATGAGA AAGAAAAAGA AGCCAAACAG AGGAAAAAGT CTCAGATTCA AGGCCGCAAA 
AAACTCAAGT CTGAATTGAA TGAGTCAGGT GAAGATTTTG GTGAAGTGCA TCAGAATTGG 
AATGCCAATA GCTCTTCAGA ACATTCGAAA GGATATCACA ACAGGGAATT CACGAGAGGC 
TTTCCGAGGG GCAGATACTC TCGAGGAAGC CATGGCAGAC CTCCGCCACA GCAGTACTAT 
AACTCAGATC CTATGGCATC TCAAGAGAGC TTGGGATTTC CACCTCAGAG ACAAGATAAT 
CCTGTCATGC CCCACTATCC TTTTCCCCCA CCCATGTTTG ATATGCATAA CTTTCCACTG 
CCGCCCCCGC CCCCACCCCC ACCAACGGTT AGCATGGGGT GGGCTGCACC TAGCATGACC 
TCTCATCCTG TTCTTAACTT ACCATACTCC CTACCCCCTC CTCCACTTCC TCCTCCCCCT 
CCTCCTCCCT CTCCTGGGGA AAGTAATTCT TCTCATTTTG GATCTTACTA TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001019943.1
CDS	79..1452
Translation	MEVVEAAAQLQTLKFGGSGPGSAASPPPEERPDAEQAEQAPPAGEQPPPAPSSDAGENPPPPSGPCATAPDAAEPLPELQDSDSSDSDSDSETDSDSSSSSSSSSSSSSSCGSLPPVLSDGEEDVQVEKENKNFPLKTKDELLLNELPSVEELTVTLPEDIALKPLGKVSSIIEQLVIIESMTNIPPVNEDTVIFKSDRQAAGKIFEIFGPVAHPFYVLRFNSSEHIESKGIKIKDTMYFAPSMKDFTQYIFTEKLKQDRGSDASWKNDQEPPPEALDFSDDEKEKEAKQRKKSQIQGRKKLKSELNESGEDFGEVHQNWNANSSSEHSKGYHNREFTRGFPRGRYSRGSHGRPPPQQYYNSDPMASQESLGFPPQRQDNPVMPHYPFPPPMFDMHNFPLPPPPPPPPTVSMGWAAPSMTSHPVLNLPYSLPPPPLPPPPPPPSPGESNSSHFGSYY

Target ORF information:

RefSeq Version	NM_001024772.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus nuclear assembly factor 1 ribonucleoprotein (Naf1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001024772.1

ORF Insert Sequence:

ATGGAGGTGG TGGAGGCTGC CGCGCAGCTG CAGACGCTCA AGTTCGGCGG CTCCGGCCCG 
GGATCCGCAG CATCGCCGCC ACCTGAGGAG CGCCCGGACG CCGAGCAGGC GGAGCAGGCC 
CCGCCGGCAG GGGAGCAGCC GCCGCCAGCC CCGAGCTCGG ATGCTGGTGA AAACCCACCG 
CCGCCGAGCG GGCCCTGCGC CACCGCACCG GACGCCGCGG AGCCGCTGCC AGAGCTGCAG 
GACTCGGACT CATCGGACTC GGATTCGGAC AGTGAAACGG ATTCGGATAG CTCAAGCTCT 
TCCTCTTCCT CATCTTCATC ATCTTCCTCC TGTGGATCGC TTCCTCCTGT GTTGTCAGAC 
GGAGAGGAGG ATGTCCAAGT TGAGAAAGAA AATAAGAATT TTCCTCTTAA AACAAAAGAT 
GAATTGCTTC TTAATGAACT ACCTTCTGTG GAAGAACTCA CTGTTACTCT GCCTGAAGAC 
ATTGCACTAA AGCCTCTTGG GAAGGTTTCG AGCATTATTG AACAGTTAGT GATAATTGAG 
TCTATGACTA ACATACCTCC AGTTAATGAG GATACTGTCA TTTTCAAAAG TGATCGACAA 
GCAGCAGGGA AGATATTTGA GATCTTTGGA CCTGTTGCCC ATCCATTTTA TGTGTTACGG 
TTCAATTCTT CAGAGCACAT TGAGAGTAAA GGTATCAAAA TAAAGGATAC TATGTATTTT 
GCTCCATCAA TGAAAGACTT CACTCAGTAT ATATTCACAG AAAAACTCAA ACAGGACAGA 
GGATCTGATG CTTCTTGGAA GAATGATCAG GAACCACCTC CAGAAGCCTT GGATTTCAGT 
GATGATGAGA AAGAAAAAGA AGCCAAACAG AGGAAAAAGT CTCAGATTCA AGGCCGCAAA 
AAACTCAAGT CTGAATTGAA TGAGTCAGGT GAAGATTTTG GTGAAGTGCA TCAGAATTGG 
AATGCCAATA GCTCTTCAGA ACATTCGAAA GGATATCACA ACAGGGAATT CACGAGAGGC 
TTTCCGAGGG GCAGATACTC TCGAGGAAGC CATGGCAGAC CTCCGCCACA GCAGTACTAT 
AACTCAGATC CTATGGCATC TCAAGAGAGC TTGGGATTTC CACCTCAGAG ACAAGATAAT 
CCTGTCATGC CCCACTATCC TTTTCCCCCA CCCATGTTTG ATATGCATAA CTTTCCACTG 
CCGCCCCCGC CCCCACCCCC ACCAACGGTT AGCATGGGGT GGGCTGCACC TAGCATGACC 
TCTCATCCTG TTCTTAACTT ACCATACTCC CTACCCCCTC CTCCACTTCC TCCTCCCCCT 
CCTCCTCCCT CTCCTGGGGA AAGTAATTCT TCTCATTTTG GATCTTACTA TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Naf1 ( XM_017600101.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017455590.1 Rattus norvegicus nuclear assembly factor 1 ribonucleoprotein (Naf1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa58801
Clone ID Related Accession (Same CDS sequence)	XM_017600101.1
Accession Version	XM_017600101.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1227bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	H/ACA ribonucleoprotein complex non-core subunit NAF1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005115.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGTGG TGGAGGCTGC CGCGCAGCTG CAGACGCTCA AGTTCGGCGG CTCCGGCCCG 
GGATCCGCAG CATCGCCGCC ACCTGAGGAG CGCCCGGACG CCGAGCAGGC GGAGCAGGCC 
CCGCCGGCAG GGGAGCAGCC GCCGCCAGCC CCGAGCTCGG ATGCTGGTGA AAACCCACCG 
CCGCCGAGCG GGCCCTGCGC CACCGCACCG GACGCCGCGG AGCCGCTGCC AGAGCTGCAG 
GACTCGGACT CATCGGACTC GGATTCGGAC AGTGAAACGG ATTCGGATAG CTCAAGCTCT 
TCCTCTTCCT CATCTTCATC ATCTTCCTCC TGTGGATCGC TTCCTCCTGT GTTGTCAGAC 
GGAGAGGAGG ATGTCCAAGT TGAGAAAGAA AATAAGAATT TTCCTCTTAA AACAAAAGAT 
GAATTGCTTC TTAATGAACT ACCTTCTGTG GAAGAACTCA CTGTTACTCT GCCTGAAGAC 
ATTGCACTAA AGCCTCTTGG GAAGGTTTCG AGCATTATTG AACAGTTAGT GATAATTGAG 
TCTATGACTA ACATACCTCC AGTTAATGAG GATACTGTCA TTTTCAAAAG TGATCGACAA 
GCAGCAGGGA AGATATTTGA GATCTTTGGA CCTGTTGCCC ATCCATTTTA TGTGTTACGG 
TTCAATTCTT CAGAGCACAT TGAGAGTAAA GGTATCAAAA TAAAGGATAC TATGTATTTT 
GCTCCATCAA TGAAAGACTT CACTCAGTAT ATATTCACAG AAAAACTCAA ACAGGACAGA 
GGATCTGATG CTTCTTGGAA GAATGATCAG GAACCACCTC CAGAAGCCTT GGATTTCAGT 
GATGATGAGA AAGAAAAAGA AGCCAAACAG AGGAAAAAGT CTCAGATTCA AGGCCGCAAA 
AAACTCAAGT CTGAATTGAA TGAGTCAGGT GAAGATTTTG GTGAAGTGCA TCAGAATTGG 
AATGCCAATA GCTCTTCAGA ACATTCGAAA GGATATCACA ACAGGGAATT CACGAGAGGC 
TTTCCGAGGG GCAGATACTC TCGAGGAAGC CATGGCAGAC CTCCGCCACA GCAGTACTAT 
AACTCAGATC CTATGGCATC TCAAGAGAGC TTGGGATTTC CACCTCAGAG ACAAGATAAT 
CCTGTCATGC CCCACTATCC TTTTCCCCAC CCATGTTTGA TATGCATAAC TTTCCACTGC 
CACCCCCCCC ACCCCCCCAA CGGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017455590.1
CDS	154..1380
Translation	MEVVEAAAQLQTLKFGGSGPGSAASPPPEERPDAEQAEQAPPAGEQPPPAPSSDAGENPPPPSGPCATAPDAAEPLPELQDSDSSDSDSDSETDSDSSSSSSSSSSSSSSCGSLPPVLSDGEEDVQVEKENKNFPLKTKDELLLNELPSVEELTVTLPEDIALKPLGKVSSIIEQLVIIESMTNIPPVNEDTVIFKSDRQAAGKIFEIFGPVAHPFYVLRFNSSEHIESKGIKIKDTMYFAPSMKDFTQYIFTEKLKQDRGSDASWKNDQEPPPEALDFSDDEKEKEAKQRKKSQIQGRKKLKSELNESGEDFGEVHQNWNANSSSEHSKGYHNREFTRGFPRGRYSRGSHGRPPPQQYYNSDPMASQESLGFPPQRQDNPVMPHYPFPHPCLICITFHCHPPHPPNG

Target ORF information:

RefSeq Version	XM_017600101.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus nuclear assembly factor 1 ribonucleoprotein (Naf1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017600101.1

ORF Insert Sequence:

ATGGAGGTGG TGGAGGCTGC CGCGCAGCTG CAGACGCTCA AGTTCGGCGG CTCCGGCCCG 
GGATCCGCAG CATCGCCGCC ACCTGAGGAG CGCCCGGACG CCGAGCAGGC GGAGCAGGCC 
CCGCCGGCAG GGGAGCAGCC GCCGCCAGCC CCGAGCTCGG ATGCTGGTGA AAACCCACCG 
CCGCCGAGCG GGCCCTGCGC CACCGCACCG GACGCCGCGG AGCCGCTGCC AGAGCTGCAG 
GACTCGGACT CATCGGACTC GGATTCGGAC AGTGAAACGG ATTCGGATAG CTCAAGCTCT 
TCCTCTTCCT CATCTTCATC ATCTTCCTCC TGTGGATCGC TTCCTCCTGT GTTGTCAGAC 
GGAGAGGAGG ATGTCCAAGT TGAGAAAGAA AATAAGAATT TTCCTCTTAA AACAAAAGAT 
GAATTGCTTC TTAATGAACT ACCTTCTGTG GAAGAACTCA CTGTTACTCT GCCTGAAGAC 
ATTGCACTAA AGCCTCTTGG GAAGGTTTCG AGCATTATTG AACAGTTAGT GATAATTGAG 
TCTATGACTA ACATACCTCC AGTTAATGAG GATACTGTCA TTTTCAAAAG TGATCGACAA 
GCAGCAGGGA AGATATTTGA GATCTTTGGA CCTGTTGCCC ATCCATTTTA TGTGTTACGG 
TTCAATTCTT CAGAGCACAT TGAGAGTAAA GGTATCAAAA TAAAGGATAC TATGTATTTT 
GCTCCATCAA TGAAAGACTT CACTCAGTAT ATATTCACAG AAAAACTCAA ACAGGACAGA 
GGATCTGATG CTTCTTGGAA GAATGATCAG GAACCACCTC CAGAAGCCTT GGATTTCAGT 
GATGATGAGA AAGAAAAAGA AGCCAAACAG AGGAAAAAGT CTCAGATTCA AGGCCGCAAA 
AAACTCAAGT CTGAATTGAA TGAGTCAGGT GAAGATTTTG GTGAAGTGCA TCAGAATTGG 
AATGCCAATA GCTCTTCAGA ACATTCGAAA GGATATCACA ACAGGGAATT CACGAGAGGC 
TTTCCGAGGG GCAGATACTC TCGAGGAAGC CATGGCAGAC CTCCGCCACA GCAGTACTAT 
AACTCAGATC CTATGGCATC TCAAGAGAGC TTGGGATTTC CACCTCAGAG ACAAGATAAT 
CCTGTCATGC CCCACTATCC TTTTCCCCAC CCATGTTTGA TATGCATAAC TTTCCACTGC 
CACCCCCCCC ACCCCCCCAA CGGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Loss-of-function mutations in the RNA biogenesis factor NAF1 predispose to pulmonary fibrosis-emphysema.
Science translational medicine8(351)351ra107(2016 08)
Stanley SE,Gable DL,Wagner CL,Carlile TM,Hanumanthu VS,Podlevsky JD,Khalil SE,DeZern AE,Rojas-Duran MF,Applegate CD,Alder JK,Parry EM,Gilbert WV,Armanios M

Proteostatic control of telomerase function through TRiC-mediated folding of TCAB1.
Cell159(6)1389-403(2014 Dec)
Freund A,Zhong FL,Venteicher AS,Meng Z,Veenstra TD,Frydman J,Artandi SE

An enhanced H/ACA RNP assembly mechanism for human telomerase RNA.
Molecular and cellular biology32(13)2428-39(2012 Jul)
Egan ED,Collins K

The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts.
Molecular cell46(5)674-90(2012 Jun)
Baltz AG,Munschauer M,Schwanh?usser B,Vasile A,Murakawa Y,Schueler M,Youngs N,Penfold-Brown D,Drew K,Milek M,Wyler E,Bonneau R,Selbach M,Dieterich C,Landthaler M

Purification of human telomerase complexes identifies factors involved in telomerase biogenesis and telomere length regulation.
Molecular cell28(5)773-85(2007 Dec)
Fu D,Collins K

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Naf1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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