Vcl cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Vcl
Entrez Gene ID	305679
Full Name	vinculin
General protein information	Preferred Names vinculin Names vinculin metavinculin
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 15 Map Location: 15p16

mRNA and Protein(s)

mRNA	Protein	Name
XM_006251639.3	XP_006251701.2	vinculin isoform X1
NM_001107248.1	NP_001100718.1	vinculin

Pathways from BioSystems

Homologies

	VCL	XP_001118118.1
Gallus gallus (chicken)	VCL	NP_990772.1
Xenopus tropicalis (tropical clawed frog)	vcl	NP_001090722.1
Bos taurus (cattle)	VCL	NP_001178299.1
Canis lupus familiaris (dog)	VCL	XP_536395.3
Danio rerio (zebrafish)	vcla	NP_001122153.1
Homo sapiens (human)	VCL	NP_054706.1
Pan troglodytes (chimpanzee)	VCL	XP_507854.2
Mus musculus (house mouse)	Vcl	NP_033528.3
Rattus norvegicus (Norway rat)	Vcl	NP_001100718.1

Gene Ontology
Bibliography

Related articles in PubMed

The WD40-domain containing protein CORO2B is specifically enriched in glomerular podocytes and regulates the ventral actin cytoskeleton.
Rogg M, Yasuda-Yamahara M, Abed A, Dinse P, Helmst?dter M, Conzelmann AC, Frimmel J, Sellung D, Biniossek ML, Kretz O, Grahammer F, Schilling O, Huber TB, Schell C
Scientific reports7(1)15910(2017 Nov)

Deletion of MLIP (muscle-enriched A-type lamin-interacting protein) leads to cardiac hyperactivation of Akt/mammalian target of rapamycin (mTOR) and impaired cardiac adaptation.
Cattin ME, Wang J, Weldrick JJ, Roeske CL, Mak E, Thorn SL, DaSilva JN, Wang Y, Lusis AJ, Burgon PG
The Journal of biological chemistry290(44)26699-714(2015 Oct)

E-cadherin interactome complexity and robustness resolved by quantitative proteomics.
Guo Z, Neilson LJ, Zhong H, Murray PS, Zanivan S, Zaidel-Bar R
Science signaling7(354)rs7(2014 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Host anti-Cytolethal distending toxin antibodies cross-react with vinculin in model of Campylobacter jejuni gastroenteritis.
Title: Autoimmunity Links Vinculin to the Pathophysiology of Chronic Functional Bowel Changes Following Campylobacter jejuni Infection in a Rat Model.

The following Vcl gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Vcl cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa58567	XM_006251639.3 Latest version!	Rattus norvegicus vinculin (Vcl), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
ORa03170	NM_001107248.1 Latest version!	Rattus norvegicus vinculin (Vcl), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Vcl ( XM_006251639.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006251701.2 Rattus norvegicus vinculin (Vcl), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa58567
Clone ID Related Accession (Same CDS sequence)	XM_006251639.3
Accession Version	XM_006251639.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3405bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	vinculin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005114.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006251639.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGGGTT TTCACACGCG CACGATCGAG AGCATCCTGG AGCCGGTGGC GCAGCAGATC 
TCTCACCTGG TGATTATGCA CGAGGAGGGC GAGGTGGACG GCAAAGCCAT TCCTGACCTC 
ACCGCGCCCG TGGCCGCTGT GCAGGCGGCC GTCAGCAACC TCGTCCGGGT TGGGAAAGAG 
ACCGTTCAGA CCACTGAGGA TCAGATTTTG AAGAGAGATA TGCCGCCAGC ATTTATTAAG 
GTCGAGAATG CTTGCACGAA GCTTGTTCAG GCAGCTCAGA TGCTTCAGTC AGACCCATAC 
TCTGTTCCTG CGCGGGATTA CCTCATCGAC GGCTCCAGGG GAATCCTCTC TGGAACGTCT 
GACCTCCTGC TTACCTTTGA TGAGGCTGAG GTTCGAAAAA TTATAAGAGT TTGCAAAGGG 
ATTTTGGAAT ACCTCACAGT GGCAGAGGTA GTGGAGACTA TGGAAGACTT GGTCACTTAC 
ACAAAGAACC TTGGGCCCGG AATGACGAAG ATGGCCAAAA TGATCGATGA GAGACAGCAG 
GAGTTGACGC ACCAGGAACA CCGTGTGATG CTGGTGAACT CAATGAACAC TGTCAAAGAG 
CTGCTTCCTG TTCTCATTTC AGCTATGAAG ATTTTTGTTA CAACCAAAAA CTCAAAAAAC 
CAAGGAATAG AAGAAGCTCT GAAAAATCGA AATTTTACTG TAGGAAAGAT GAGTGCTGAG 
ATTAACGAGA TCATTCGTGT GTTACAACTC ACCTCCTGGG ATGAGGACGC CTGGGCCAGC 
AAGGACACTG AAGCCATGAA GAGAGCGCTG GCCTCCATAG ACTCGAAACT GAACCAGGCC 
AAAGGTTGGC TCCGTGACCC CAACGCTTCC CCAGGGGACG CCGGTGAGCA GGCCATCAGG 
CAGATCTTGG ACGAAGCTGG AAAAGTTGGT GAACTTTGTG CAGGCAAGGA ACGCAGGGAG 
ATCCTAGGAA CCTGCAAGAT GCTAGGGCAG ATGACTGACC AAGTGGCTGA CCTCCGAGCC 
AGAGGACAAG GAGCCTCGCC AGTGGCCATG CAGAAGGCCC AGCAAGTGTC TCAGGGGCTC 
GACGTGCTTA CTGCGAAAGT GGAAAATGCA GCTCGAAAGC TGGAAGCCAT GACGAACTCA 
AAGCAGAGTA TTGCGAAGAA GATTGACGCT GCGCAGAATT GGCTGGCAGA TCCCAATGGT 
GGACCTGAAG GAGAAGAACA GATCCGAGGG GCTTTGGCTG AAGCTCGGAA GATTGCTGAG 
TTATGTGATG ATCCTAAGGA GAGGGACGAC ATCCTCCGCT CTCTTGGAGA GATAGCGGCT 
CTGACCTCTA AATTAGGAGA CTTGCGAAGA CAGGGGAAAG GAGACTCCCC AGAGGCTCGA 
GCCTTGGCTA AACAAGTGGC AACCGCGCTA CAGAACCTGC AGACCAAAAC CAATAGGGCT 
GTGGCCAACA GCAGGCCTGC CAAGGCAGCC GTCCACCTGG AAGGCAAGAT TGAGCAGGCA 
CAGCGGTGGA TTGATAACCC TACAGTGGAT GACCGAGGAG TCGGTCAGGC TGCCATCCGT 
GGACTTGTGG CTGAAGGGCA TCGGCTGGCC AATGTCATGA TGGGACCTTA TCGCCAAGAT 
CTTCTCGCCA AGTGTGACCG TGTAGACCAG CTAGCAGCTC AGCTGGCTGA CCTGGCTGCC 
CGAGGGGAGG GGGAGAGTCC TCAGGCGAGA GCGCTTGCTT CTCAGCTTCA GGACTCCTTA 
AAGGATCTTA AAACCCAGAT GCAGGAAGCT ATGACTCAAG AGGTGTCCGA CGTTTTCAGC 
GATACTACAA CTCCCATCAA GCTGTTGGCA GTGGCGGCCA CTGCTCCTCC TGATGCGCCC 
AATAGGGAAG AGGTATTTGA CGAGAGAGCA GCCAACTTTG AAAACCATTC AGGAAGGCTT 
GGGGCCACGG CAGAGAAGGC AGCTGCTGTT GGTGCTGCCA ATAAATCGAC AGTGGAAGGC 
ATTCAGGCAT CCGTGAAGAC AGCCCGAGAA CTCACTCCCC AGGTGATCTC AGCTGCTCGA 
ATCTTACTGA GGAACCCTGG TAACCAAGCT GCGTATGAAC ATTTTGAGAC CATGAAGAAC 
CAGTGGATTG ATAATGTTGA GAAAATGACA GGGCTGGTGG ACGAGGCCAT TGATACCAAA 
TCTCTGTTGG ATGCTTCTGA AGAAGCAATT AAAAAAGACC TGGACAAGTG TAAGGTAGCC 
ATGGCCAATA TTCAGCCTCA GATGCTGGTC GCTGGAGCAA CCAGTATTGC TCGTCGGGCC 
AACCGGATTC TGCTGGTTGC TAAGAGGGAG GTAGAGAACT CTGAGGATCC GAAGTTCCGA 
GAGGCTGTGA AAGCTGCCTC TGATGAACTG AGCAAAACAA TCTCCCCCAT GGTTATGGAT 
GCCAAGGCTG TGGCTGGAAA CATCTCTGAC CCTGGCCTGC AAAAGAGCTT CCTGGACTCA 
GGATATCGGA TCCTGGGAGC TGTGGCCAAA GTTAGAGAAG CCTTCCAACC TCAGGAGCCT 
GACTTCCCGC CTCCTCCACC AGACCTTGAA CAGCTACGTC TAACTGATGA GCTGGCTCCT 
CCCAAACCAC CTCTGCCTGA GGGTGAAGTC CCTCCACCCA GGCCACCCCC ACCAGAAGAG 
AAGGATGAAG AGTTCCCTGA GCAGAAAGCC GGGGAGGTGA TTAACCAGCC GATGATGATG 
GCCGCCAGGC AGCTCCACGA TGAAGCTCGG AAGTGGTCTA GCAAGCCGGG TCTCCCAGCC 
GCTCAGGTGG GTATAGGAGT TGTAGCTGAG GCAGATGCGG CTGATGCTGT TGGGTTCCCT 
TTCCCCTCTG ACATGGAAGA CGATTCAGAA CCTGAGCTGC TGTTAGTGCC AGCTAATCAG 
CCGGTCAACC AGCCCATTCT GGCCGCGGCT CAGTCCTTGC ACCGGGAAGC TACCAAGTGG 
TCTAGTAAGG GCAATGACAT CATCGCAGCA GCCAAGCGCA TGGCTCTGCT GATGGCGGAG 
ATGTCTCGGC TGGTAAGAGG GGGCAGTGGT ACCAAGCGGG CACTTATTCA GTGTGCCAAG 
GATATCGCCA AGGCCTCTGA TGAGGTGACG AGGTTGGCCA AGGAGGTTGC CAAGCAGTGC 
ACAGATAAGC GGATTAGAAC CAATCTCTTA CAGGTCTGTG AGCGAATCCC AACTATAAGC 
ACCCAGCTCA AAATCCTGTC CACAGTGAAG GCCACCATGC TGGGCCGGAC CAACATCAGT 
GACGAGGAGT CTGAGCAGGC CACAGAGATG CTGGTTCATA ATGCCCAGAA CCTCATGCAG 
TCTGTGAAAG AGACTGTGCG AGAGGCCGAA GCTGCTTCAA TCAAGATTCG AACAGACGCC 
GGATTTACTC TGCGCTGGGT CAGAAAGACT CCCTGGTACC AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006251701.2
CDS	142..3546
Translation	MPGFHTRTIESILEPVAQQISHLVIMHEEGEVDGKAIPDLTAPVAAVQAAVSNLVRVGKETVQTTEDQILKRDMPPAFIKVENACTKLVQAAQMLQSDPYSVPARDYLIDGSRGILSGTSDLLLTFDEAEVRKIIRVCKGILEYLTVAEVVETMEDLVTYTKNLGPGMTKMAKMIDERQQELTHQEHRVMLVNSMNTVKELLPVLISAMKIFVTTKNSKNQGIEEALKNRNFTVGKMSAEINEIIRVLQLTSWDEDAWASKDTEAMKRALASIDSKLNQAKGWLRDPNASPGDAGEQAIRQILDEAGKVGELCAGKERREILGTCKMLGQMTDQVADLRARGQGASPVAMQKAQQVSQGLDVLTAKVENAARKLEAMTNSKQSIAKKIDAAQNWLADPNGGPEGEEQIRGALAEARKIAELCDDPKERDDILRSLGEIAALTSKLGDLRRQGKGDSPEARALAKQVATALQNLQTKTNRAVANSRPAKAAVHLEGKIEQAQRWIDNPTVDDRGVGQAAIRGLVAEGHRLANVMMGPYRQDLLAKCDRVDQLAAQLADLAARGEGESPQARALASQLQDSLKDLKTQMQEAMTQEVSDVFSDTTTPIKLLAVAATAPPDAPNREEVFDERAANFENHSGRLGATAEKAAAVGAANKSTVEGIQASVKTARELTPQVISAARILLRNPGNQAAYEHFETMKNQWIDNVEKMTGLVDEAIDTKSLLDASEEAIKKDLDKCKVAMANIQPQMLVAGATSIARRANRILLVAKREVENSEDPKFREAVKAASDELSKTISPMVMDAKAVAGNISDPGLQKSFLDSGYRILGAVAKVREAFQPQEPDFPPPPPDLEQLRLTDELAPPKPPLPEGEVPPPRPPPPEEKDEEFPEQKAGEVINQPMMMAARQLHDEARKWSSKPGLPAAQVGIGVVAEADAADAVGFPFPSDMEDDSEPELLLVPANQPVNQPILAAAQSLHREATKWSSKGNDIIAAAKRMALLMAEMSRLVRGGSGTKRALIQCAKDIAKASDEVTRLAKEVAKQCTDKRIRTNLLQVCERIPTISTQLKILSTVKATMLGRTNISDEESEQATEMLVHNAQNLMQSVKETVREAEAASIKIRTDAGFTLRWVRKTPWYQ

Target ORF information:

RefSeq Version	XM_006251639.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus vinculin (Vcl), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006251639.3

ORF Insert Sequence:

ATGCCGGGTT TTCACACGCG CACGATCGAG AGCATCCTGG AGCCGGTGGC GCAGCAGATC 
TCTCACCTGG TGATTATGCA CGAGGAGGGC GAGGTGGACG GCAAAGCCAT TCCTGACCTC 
ACCGCGCCCG TGGCCGCTGT GCAGGCGGCC GTCAGCAACC TCGTCCGGGT TGGGAAAGAG 
ACCGTTCAGA CCACTGAGGA TCAGATTTTG AAGAGAGATA TGCCGCCAGC ATTTATTAAG 
GTCGAGAATG CTTGCACGAA GCTTGTTCAG GCAGCTCAGA TGCTTCAGTC AGACCCATAC 
TCTGTTCCTG CGCGGGATTA CCTCATCGAC GGCTCCAGGG GAATCCTCTC TGGAACGTCT 
GACCTCCTGC TTACCTTTGA TGAGGCTGAG GTTCGAAAAA TTATAAGAGT TTGCAAAGGG 
ATTTTGGAAT ACCTCACAGT GGCAGAGGTA GTGGAGACTA TGGAAGACTT GGTCACTTAC 
ACAAAGAACC TTGGGCCCGG AATGACGAAG ATGGCCAAAA TGATCGATGA GAGACAGCAG 
GAGTTGACGC ACCAGGAACA CCGTGTGATG CTGGTGAACT CAATGAACAC TGTCAAAGAG 
CTGCTTCCTG TTCTCATTTC AGCTATGAAG ATTTTTGTTA CAACCAAAAA CTCAAAAAAC 
CAAGGAATAG AAGAAGCTCT GAAAAATCGA AATTTTACTG TAGGAAAGAT GAGTGCTGAG 
ATTAACGAGA TCATTCGTGT GTTACAACTC ACCTCCTGGG ATGAGGACGC CTGGGCCAGC 
AAGGACACTG AAGCCATGAA GAGAGCGCTG GCCTCCATAG ACTCGAAACT GAACCAGGCC 
AAAGGTTGGC TCCGTGACCC CAACGCTTCC CCAGGGGACG CCGGTGAGCA GGCCATCAGG 
CAGATCTTGG ACGAAGCTGG AAAAGTTGGT GAACTTTGTG CAGGCAAGGA ACGCAGGGAG 
ATCCTAGGAA CCTGCAAGAT GCTAGGGCAG ATGACTGACC AAGTGGCTGA CCTCCGAGCC 
AGAGGACAAG GAGCCTCGCC AGTGGCCATG CAGAAGGCCC AGCAAGTGTC TCAGGGGCTC 
GACGTGCTTA CTGCGAAAGT GGAAAATGCA GCTCGAAAGC TGGAAGCCAT GACGAACTCA 
AAGCAGAGTA TTGCGAAGAA GATTGACGCT GCGCAGAATT GGCTGGCAGA TCCCAATGGT 
GGACCTGAAG GAGAAGAACA GATCCGAGGG GCTTTGGCTG AAGCTCGGAA GATTGCTGAG 
TTATGTGATG ATCCTAAGGA GAGGGACGAC ATCCTCCGCT CTCTTGGAGA GATAGCGGCT 
CTGACCTCTA AATTAGGAGA CTTGCGAAGA CAGGGGAAAG GAGACTCCCC AGAGGCTCGA 
GCCTTGGCTA AACAAGTGGC AACCGCGCTA CAGAACCTGC AGACCAAAAC CAATAGGGCT 
GTGGCCAACA GCAGGCCTGC CAAGGCAGCC GTCCACCTGG AAGGCAAGAT TGAGCAGGCA 
CAGCGGTGGA TTGATAACCC TACAGTGGAT GACCGAGGAG TCGGTCAGGC TGCCATCCGT 
GGACTTGTGG CTGAAGGGCA TCGGCTGGCC AATGTCATGA TGGGACCTTA TCGCCAAGAT 
CTTCTCGCCA AGTGTGACCG TGTAGACCAG CTAGCAGCTC AGCTGGCTGA CCTGGCTGCC 
CGAGGGGAGG GGGAGAGTCC TCAGGCGAGA GCGCTTGCTT CTCAGCTTCA GGACTCCTTA 
AAGGATCTTA AAACCCAGAT GCAGGAAGCT ATGACTCAAG AGGTGTCCGA CGTTTTCAGC 
GATACTACAA CTCCCATCAA GCTGTTGGCA GTGGCGGCCA CTGCTCCTCC TGATGCGCCC 
AATAGGGAAG AGGTATTTGA CGAGAGAGCA GCCAACTTTG AAAACCATTC AGGAAGGCTT 
GGGGCCACGG CAGAGAAGGC AGCTGCTGTT GGTGCTGCCA ATAAATCGAC AGTGGAAGGC 
ATTCAGGCAT CCGTGAAGAC AGCCCGAGAA CTCACTCCCC AGGTGATCTC AGCTGCTCGA 
ATCTTACTGA GGAACCCTGG TAACCAAGCT GCGTATGAAC ATTTTGAGAC CATGAAGAAC 
CAGTGGATTG ATAATGTTGA GAAAATGACA GGGCTGGTGG ACGAGGCCAT TGATACCAAA 
TCTCTGTTGG ATGCTTCTGA AGAAGCAATT AAAAAAGACC TGGACAAGTG TAAGGTAGCC 
ATGGCCAATA TTCAGCCTCA GATGCTGGTC GCTGGAGCAA CCAGTATTGC TCGTCGGGCC 
AACCGGATTC TGCTGGTTGC TAAGAGGGAG GTAGAGAACT CTGAGGATCC GAAGTTCCGA 
GAGGCTGTGA AAGCTGCCTC TGATGAACTG AGCAAAACAA TCTCCCCCAT GGTTATGGAT 
GCCAAGGCTG TGGCTGGAAA CATCTCTGAC CCTGGCCTGC AAAAGAGCTT CCTGGACTCA 
GGATATCGGA TCCTGGGAGC TGTGGCCAAA GTTAGAGAAG CCTTCCAACC TCAGGAGCCT 
GACTTCCCGC CTCCTCCACC AGACCTTGAA CAGCTACGTC TAACTGATGA GCTGGCTCCT 
CCCAAACCAC CTCTGCCTGA GGGTGAAGTC CCTCCACCCA GGCCACCCCC ACCAGAAGAG 
AAGGATGAAG AGTTCCCTGA GCAGAAAGCC GGGGAGGTGA TTAACCAGCC GATGATGATG 
GCCGCCAGGC AGCTCCACGA TGAAGCTCGG AAGTGGTCTA GCAAGCCGGG TCTCCCAGCC 
GCTCAGGTGG GTATAGGAGT TGTAGCTGAG GCAGATGCGG CTGATGCTGT TGGGTTCCCT 
TTCCCCTCTG ACATGGAAGA CGATTCAGAA CCTGAGCTGC TGTTAGTGCC AGCTAATCAG 
CCGGTCAACC AGCCCATTCT GGCCGCGGCT CAGTCCTTGC ACCGGGAAGC TACCAAGTGG 
TCTAGTAAGG GCAATGACAT CATCGCAGCA GCCAAGCGCA TGGCTCTGCT GATGGCGGAG 
ATGTCTCGGC TGGTAAGAGG GGGCAGTGGT ACCAAGCGGG CACTTATTCA GTGTGCCAAG 
GATATCGCCA AGGCCTCTGA TGAGGTGACG AGGTTGGCCA AGGAGGTTGC CAAGCAGTGC 
ACAGATAAGC GGATTAGAAC CAATCTCTTA CAGGTCTGTG AGCGAATCCC AACTATAAGC 
ACCCAGCTCA AAATCCTGTC CACAGTGAAG GCCACCATGC TGGGCCGGAC CAACATCAGT 
GACGAGGAGT CTGAGCAGGC CACAGAGATG CTGGTTCATA ATGCCCAGAA CCTCATGCAG 
TCTGTGAAAG AGACTGTGCG AGAGGCCGAA GCTGCTTCAA TCAAGATTCG AACAGACGCC 
GGATTTACTC TGCGCTGGGT CAGAAAGACT CCCTGGTACC AGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Vcl ( NM_001107248.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001100718.1 Rattus norvegicus vinculin (Vcl), mRNA.

Price & Availability↑

CloneID	ORa03170
Clone ID Related Accession (Same CDS sequence)	NM_001107248.1
Accession Version	NM_001107248.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3201bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-12
Organism	Rattus norvegicus(Norway rat)
Product	vinculin
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CH474061.1. On or before Oct 4, 2007 this sequence version replaced XM_223781.4, XM_001057629.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END##

ATGCCGGTGT TTCACACGCG CACGATCGAG AGCATCCTGG AGCCGGTGGC GCAGCAGATC 
TCTCACCTGG TGATTATGCA CGAGGAGGGC GAGGTGGACG GCAAAGCCAT TCCTGACCTC 
ACCGCGCCCG TGGCCGCTGT GCAGGCGGCC GTCAGCAACC TCGTCCGGGT TGGGAAAGAG 
ACCGTTCAGA CCACTGAGGA TCAGATTTTG AAGAGAGATA TGCCGCCAGC ATTTATTAAG 
GTCGAGAATG CTTGCACGAA GCTTGTTCAG GCAGCTCAGA TGCTTCAGTC AGACCCATAC 
TCTGTTCCTG CGCGGGATTA CCTCATCGAC GGCTCCAGGG GAATCCTCTC TGGAACGTCT 
GACCTCCTGC TTACCTTTGA TGAGGCTGAG GTTCGAAAAA TTATAAGAGT TTGCAAAGGG 
ATTTTGGAAT ACCTCACAGT GGCAGAGGTA GTGGAGACTA TGGAAGACTT GGTCACTTAC 
ACAAAGAACC TTGGGCCCGG AATGACGAAG ATGGCCAAAA TGATCGATGA GAGACAGCAG 
GAGTTGACGC ACCAGGAACA CCGTGTGATG CTGGTGAACT CAATGAACAC TGTCAAAGAG 
CTGCTTCCTG TTCTCATTTC AGCTATGAAG ATTTTTGTTA CAACCAAAAA CTCAAAAAAC 
CAAGGAATAG AAGAAGCTCT GAAAAATCGA AATTTTACTG TAGGAAAGAT GAGTGCTGAG 
ATTAACGAGA TCATTCGTGT GTTACAACTC ACCTCCTGGG ATGAGGACGC CTGGGCCAGC 
AAGGACACTG AAGCCATGAA GAGAGCGCTG GCCTCCATAG ACTCGAAACT GAACCAGGCC 
AAAGGTTGGC TCCGTGACCC CAACGCTTCC CCAGGGGACG CCGGTGAGCA GGCCATCAGG 
CAGATCTTGG ACGAAGCTGG AAAAGTTGGT GAACTTTGTG CAGGCAAGGA ACGCAGGGAG 
ATCCTAGGAA CCTGCAAGAT GCTAGGGCAG ATGACTGACC AAGTGGCTGA CCTCCGAGCC 
AGAGGACAAG GAGCCTCGCC AGTGGCCATG CAGAAGGCCC AGCAAGTGTC TCAGGGGCTC 
GACGTGCTTA CTGCGAAAGT GGAAAATGCA GCTCGAAAGC TGGAAGCCAT GACGAACTCA 
AAGCAGAGTA TTGCGAAGAA GATTGACGCT GCGCAGAATT GGCTGGCAGA TCCCAATGGT 
GGACCTGAAG GAGAAGAACA GATCCGAGGG GCTTTGGCTG AAGCTCGGAA GATTGCTGAG 
TTATGTGATG ATCCTAAGGA GAGGGACGAC ATCCTCCGCT CTCTTGGAGA GATAGCGGCT 
CTGACCTCTA AATTAGGAGA CTTGCGAAGA CAGGGGAAAG GAGACTCCCC AGAGGCTCGA 
GCCTTGGCTA AACAAGTGGC AACCGCGCTA CAGAACCTGC AGACCAAAAC CAATAGGGCT 
GTGGCCAACA GCAGGCCTGC CAAGGCAGCC GTCCACCTGG AAGGCAAGAT TGAGCAGGCA 
CAGCGGTGGA TTGATAACCC TACAGTGGAT GACCGAGGAG TCGGTCAGGC TGCCATCCGT 
GGACTTGTGG CTGAAGGGCA TCGGCTGGCC AATGTCATGA TGGGACCTTA TCGCCAAGAT 
CTTCTCGCCA AGTGTGACCG TGTAGACCAG CTAGCAGCTC AGCTGGCTGA CCTGGCTGCC 
CGAGGGGAGG GGGAGAGTCC TCAGGCGAGA GCGCTTGCTT CTCAGCTTCA GGACTCCTTA 
AAGGATCTTA AAACCCAGAT GCAGGAAGCT ATGACTCAAG AGGTGTCCGA CGTTTTCAGC 
GATACTACAA CTCCCATCAA GCTGTTGGCA GTGGCGGCCA CTGCTCCTCC TGATGCGCCC 
AATAGGGAAG AGGTATTTGA CGAGAGAGCA GCCAACTTTG AAAACCATTC AGGAAGGCTT 
GGGGCCACGG CAGAGAAGGC AGCTGCTGTT GGTGCTGCCA ATAAATCGAC AGTGGAAGGC 
ATTCAGGCAT CCGTGAAGAC AGCCCGAGAA CTCACTCCCC AGGTGATCTC AGCTGCTCGA 
ATCTTACTGA GGAACCCTGG TAACCAAGCT GCGTATGAAC ATTTTGAGAC CATGAAGAAC 
CAGTGGATTG ATAATGTTGA GAAAATGACA GGGCTGGTGG ACGAGGCCAT TGATACCAAA 
TCTCTGTTGG ATGCTTCTGA AGAAGCAATT AAAAAAGACC TGGACAAGTG TAAGGTAGCC 
ATGGCCAATA TTCAGCCTCA GATGCTGGTC GCTGGAGCAA CCAGTATTGC TCGTCGGGCC 
AACCGGATTC TGCTGGTTGC TAAGAGGGAG GTAGAGAACT CTGAGGATCC GAAGTTCCGA 
GAGGCTGTGA AAGCTGCCTC TGATGAACTG AGCAAAACAA TCTCCCCCAT GGTTATGGAT 
GCCAAGGCTG TGGCTGGAAA CATCTCTGAC CCTGGCCTGC AAAAGAGCTT CCTGGACTCA 
GGATATCGGA TCCTGGGAGC TGTGGCCAAA GTTAGAGAAG CCTTCCAACC TCAGGAGCCT 
GACTTCCCGC CTCCTCCACC AGACCTTGAA CAGCTACGTC TAACTGATGA GCTGGCTCCT 
CCCAAACCAC CTCTGCCTGA GGGTGAAGTC CCTCCACCCA GGCCACCCCC ACCAGAAGAG 
AAGGATGAAG AGTTCCCTGA GCAGAAAGCC GGGGAGGTGA TTAACCAGCC GATGATGATG 
GCCGCCAGGC AGCTCCACGA TGAAGCTCGG AAGTGGTCTA GCAAGGGCAA TGACATCATC 
GCAGCAGCCA AGCGCATGGC TCTGCTGATG GCGGAGATGT CTCGGCTGGT AAGAGGGGGC 
AGTGGTACCA AGCGGGCACT TATTCAGTGT GCCAAGGATA TCGCCAAGGC CTCTGATGAG 
GTGACGAGGT TGGCCAAGGA GGTTGCCAAG CAGTGCACAG ATAAGCGGAT TAGAACCAAT 
CTCTTACAGG TCTGTGAGCG AATCCCAACT ATAAGCACCC AGCTCAAAAT CCTGTCCACA 
GTGAAGGCCA CCATGCTGGG CCGGACCAAC ATCAGTGACG AGGAGTCTGA GCAGGCCACA 
GAGATGCTGG TTCATAATGC CCAGAACCTC ATGCAGTCTG TGAAAGAGAC TGTGCGAGAG 
GCCGAAGCTG CTTCAATCAA GATTCGAACA GACGCCGGAT TTACTCTGCG CTGGGTCAGA 
AAGACTCCCT GGTACCAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001100718.1
CDS	89..3289
Translation	MPVFHTRTIESILEPVAQQISHLVIMHEEGEVDGKAIPDLTAPVAAVQAAVSNLVRVGKETVQTTEDQILKRDMPPAFIKVENACTKLVQAAQMLQSDPYSVPARDYLIDGSRGILSGTSDLLLTFDEAEVRKIIRVCKGILEYLTVAEVVETMEDLVTYTKNLGPGMTKMAKMIDERQQELTHQEHRVMLVNSMNTVKELLPVLISAMKIFVTTKNSKNQGIEEALKNRNFTVGKMSAEINEIIRVLQLTSWDEDAWASKDTEAMKRALASIDSKLNQAKGWLRDPNASPGDAGEQAIRQILDEAGKVGELCAGKERREILGTCKMLGQMTDQVADLRARGQGASPVAMQKAQQVSQGLDVLTAKVENAARKLEAMTNSKQSIAKKIDAAQNWLADPNGGPEGEEQIRGALAEARKIAELCDDPKERDDILRSLGEIAALTSKLGDLRRQGKGDSPEARALAKQVATALQNLQTKTNRAVANSRPAKAAVHLEGKIEQAQRWIDNPTVDDRGVGQAAIRGLVAEGHRLANVMMGPYRQDLLAKCDRVDQLAAQLADLAARGEGESPQARALASQLQDSLKDLKTQMQEAMTQEVSDVFSDTTTPIKLLAVAATAPPDAPNREEVFDERAANFENHSGRLGATAEKAAAVGAANKSTVEGIQASVKTARELTPQVISAARILLRNPGNQAAYEHFETMKNQWIDNVEKMTGLVDEAIDTKSLLDASEEAIKKDLDKCKVAMANIQPQMLVAGATSIARRANRILLVAKREVENSEDPKFREAVKAASDELSKTISPMVMDAKAVAGNISDPGLQKSFLDSGYRILGAVAKVREAFQPQEPDFPPPPPDLEQLRLTDELAPPKPPLPEGEVPPPRPPPPEEKDEEFPEQKAGEVINQPMMMAARQLHDEARKWSSKGNDIIAAAKRMALLMAEMSRLVRGGSGTKRALIQCAKDIAKASDEVTRLAKEVAKQCTDKRIRTNLLQVCERIPTISTQLKILSTVKATMLGRTNISDEESEQATEMLVHNAQNLMQSVKETVREAEAASIKIRTDAGFTLRWVRKTPWYQ

Target ORF information:

RefSeq Version	NM_001107248.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus vinculin (Vcl), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001107248.1

ORF Insert Sequence:

ATGCCGGTGT TTCACACGCG CACGATCGAG AGCATCCTGG AGCCGGTGGC GCAGCAGATC 
TCTCACCTGG TGATTATGCA CGAGGAGGGC GAGGTGGACG GCAAAGCCAT TCCTGACCTC 
ACCGCGCCCG TGGCCGCTGT GCAGGCGGCC GTCAGCAACC TCGTCCGGGT TGGGAAAGAG 
ACCGTTCAGA CCACTGAGGA TCAGATTTTG AAGAGAGATA TGCCGCCAGC ATTTATTAAG 
GTCGAGAATG CTTGCACGAA GCTTGTTCAG GCAGCTCAGA TGCTTCAGTC AGACCCATAC 
TCTGTTCCTG CGCGGGATTA CCTCATCGAC GGCTCCAGGG GAATCCTCTC TGGAACGTCT 
GACCTCCTGC TTACCTTTGA TGAGGCTGAG GTTCGAAAAA TTATAAGAGT TTGCAAAGGG 
ATTTTGGAAT ACCTCACAGT GGCAGAGGTA GTGGAGACTA TGGAAGACTT GGTCACTTAC 
ACAAAGAACC TTGGGCCCGG AATGACGAAG ATGGCCAAAA TGATCGATGA GAGACAGCAG 
GAGTTGACGC ACCAGGAACA CCGTGTGATG CTGGTGAACT CAATGAACAC TGTCAAAGAG 
CTGCTTCCTG TTCTCATTTC AGCTATGAAG ATTTTTGTTA CAACCAAAAA CTCAAAAAAC 
CAAGGAATAG AAGAAGCTCT GAAAAATCGA AATTTTACTG TAGGAAAGAT GAGTGCTGAG 
ATTAACGAGA TCATTCGTGT GTTACAACTC ACCTCCTGGG ATGAGGACGC CTGGGCCAGC 
AAGGACACTG AAGCCATGAA GAGAGCGCTG GCCTCCATAG ACTCGAAACT GAACCAGGCC 
AAAGGTTGGC TCCGTGACCC CAACGCTTCC CCAGGGGACG CCGGTGAGCA GGCCATCAGG 
CAGATCTTGG ACGAAGCTGG AAAAGTTGGT GAACTTTGTG CAGGCAAGGA ACGCAGGGAG 
ATCCTAGGAA CCTGCAAGAT GCTAGGGCAG ATGACTGACC AAGTGGCTGA CCTCCGAGCC 
AGAGGACAAG GAGCCTCGCC AGTGGCCATG CAGAAGGCCC AGCAAGTGTC TCAGGGGCTC 
GACGTGCTTA CTGCGAAAGT GGAAAATGCA GCTCGAAAGC TGGAAGCCAT GACGAACTCA 
AAGCAGAGTA TTGCGAAGAA GATTGACGCT GCGCAGAATT GGCTGGCAGA TCCCAATGGT 
GGACCTGAAG GAGAAGAACA GATCCGAGGG GCTTTGGCTG AAGCTCGGAA GATTGCTGAG 
TTATGTGATG ATCCTAAGGA GAGGGACGAC ATCCTCCGCT CTCTTGGAGA GATAGCGGCT 
CTGACCTCTA AATTAGGAGA CTTGCGAAGA CAGGGGAAAG GAGACTCCCC AGAGGCTCGA 
GCCTTGGCTA AACAAGTGGC AACCGCGCTA CAGAACCTGC AGACCAAAAC CAATAGGGCT 
GTGGCCAACA GCAGGCCTGC CAAGGCAGCC GTCCACCTGG AAGGCAAGAT TGAGCAGGCA 
CAGCGGTGGA TTGATAACCC TACAGTGGAT GACCGAGGAG TCGGTCAGGC TGCCATCCGT 
GGACTTGTGG CTGAAGGGCA TCGGCTGGCC AATGTCATGA TGGGACCTTA TCGCCAAGAT 
CTTCTCGCCA AGTGTGACCG TGTAGACCAG CTAGCAGCTC AGCTGGCTGA CCTGGCTGCC 
CGAGGGGAGG GGGAGAGTCC TCAGGCGAGA GCGCTTGCTT CTCAGCTTCA GGACTCCTTA 
AAGGATCTTA AAACCCAGAT GCAGGAAGCT ATGACTCAAG AGGTGTCCGA CGTTTTCAGC 
GATACTACAA CTCCCATCAA GCTGTTGGCA GTGGCGGCCA CTGCTCCTCC TGATGCGCCC 
AATAGGGAAG AGGTATTTGA CGAGAGAGCA GCCAACTTTG AAAACCATTC AGGAAGGCTT 
GGGGCCACGG CAGAGAAGGC AGCTGCTGTT GGTGCTGCCA ATAAATCGAC AGTGGAAGGC 
ATTCAGGCAT CCGTGAAGAC AGCCCGAGAA CTCACTCCCC AGGTGATCTC AGCTGCTCGA 
ATCTTACTGA GGAACCCTGG TAACCAAGCT GCGTATGAAC ATTTTGAGAC CATGAAGAAC 
CAGTGGATTG ATAATGTTGA GAAAATGACA GGGCTGGTGG ACGAGGCCAT TGATACCAAA 
TCTCTGTTGG ATGCTTCTGA AGAAGCAATT AAAAAAGACC TGGACAAGTG TAAGGTAGCC 
ATGGCCAATA TTCAGCCTCA GATGCTGGTC GCTGGAGCAA CCAGTATTGC TCGTCGGGCC 
AACCGGATTC TGCTGGTTGC TAAGAGGGAG GTAGAGAACT CTGAGGATCC GAAGTTCCGA 
GAGGCTGTGA AAGCTGCCTC TGATGAACTG AGCAAAACAA TCTCCCCCAT GGTTATGGAT 
GCCAAGGCTG TGGCTGGAAA CATCTCTGAC CCTGGCCTGC AAAAGAGCTT CCTGGACTCA 
GGATATCGGA TCCTGGGAGC TGTGGCCAAA GTTAGAGAAG CCTTCCAACC TCAGGAGCCT 
GACTTCCCGC CTCCTCCACC AGACCTTGAA CAGCTACGTC TAACTGATGA GCTGGCTCCT 
CCCAAACCAC CTCTGCCTGA GGGTGAAGTC CCTCCACCCA GGCCACCCCC ACCAGAAGAG 
AAGGATGAAG AGTTCCCTGA GCAGAAAGCC GGGGAGGTGA TTAACCAGCC GATGATGATG 
GCCGCCAGGC AGCTCCACGA TGAAGCTCGG AAGTGGTCTA GCAAGGGCAA TGACATCATC 
GCAGCAGCCA AGCGCATGGC TCTGCTGATG GCGGAGATGT CTCGGCTGGT AAGAGGGGGC 
AGTGGTACCA AGCGGGCACT TATTCAGTGT GCCAAGGATA TCGCCAAGGC CTCTGATGAG 
GTGACGAGGT TGGCCAAGGA GGTTGCCAAG CAGTGCACAG ATAAGCGGAT TAGAACCAAT 
CTCTTACAGG TCTGTGAGCG AATCCCAACT ATAAGCACCC AGCTCAAAAT CCTGTCCACA 
GTGAAGGCCA CCATGCTGGG CCGGACCAAC ATCAGTGACG AGGAGTCTGA GCAGGCCACA 
GAGATGCTGG TTCATAATGC CCAGAACCTC ATGCAGTCTG TGAAAGAGAC TGTGCGAGAG 
GCCGAAGCTG CTTCAATCAA GATTCGAACA GACGCCGGAT TTACTCTGCG CTGGGTCAGA 
AAGACTCCCT GGTACCAGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The WD40-domain containing protein CORO2B is specifically enriched in glomerular podocytes and regulates the ventral actin cytoskeleton.
Scientific reports7(1)15910(2017 Nov)
Rogg M,Yasuda-Yamahara M,Abed A,Dinse P,Helmst?dter M,Conzelmann AC,Frimmel J,Sellung D,Biniossek ML,Kretz O,Grahammer F,Schilling O,Huber TB,Schell C

Deletion of MLIP (muscle-enriched A-type lamin-interacting protein) leads to cardiac hyperactivation of Akt/mammalian target of rapamycin (mTOR) and impaired cardiac adaptation.
The Journal of biological chemistry290(44)26699-714(2015 Oct)
Cattin ME,Wang J,Weldrick JJ,Roeske CL,Mak E,Thorn SL,DaSilva JN,Wang Y,Lusis AJ,Burgon PG

E-cadherin interactome complexity and robustness resolved by quantitative proteomics.
Science signaling7(354)rs7(2014 Dec)
Guo Z,Neilson LJ,Zhong H,Murray PS,Zanivan S,Zaidel-Bar R

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Vcl cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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